RECENT ADVANCES IN DOPING ANALYSIS (5)

Transcription

1 W. SCHANZER H. GEYER A. GOTZMANN U. MARECK-ENGELKE (EDITORS) RECENT ADVANCES IN DOPING ANALYSIS (5) Proceedings of the Manfred Donike Workshop 15th Cologne Workshop on Dope Analysis 23rd to 28th February 1997 SPORT und BUCH StrauB

2 Table of Contents I Anabolic Agents W. SCHANZER, H. GEYER, S. HORNING: Long-term Determination of Metandienone and Mestanolone 13 H. SAUERWEIN, A. GLEIXNER, H.H.D. MEYER: Clenbuterol: Longterm Detection via Hair Analysis 27 D. DEBOER, K.M. HOLD, D.J. CROUCH, D.G. WILKINS, D.E. ROLLINS, R.A.A. MAES: The Potential Use of Hair in Doping Control in General and in the Detection of Stanozolol in Particular 41 A.T. KICMAN, S.B. COUTTS, D.J. HANDELSMAN, F.C.W. WU, D. A. Cow AN: Adrenal Gland Contribution to Urinary Epitestosterone in Men 49 II Steroid Profiling U. MARECK-ENGELKE, H. GEYER, W. SCHANZER: The Interpretation of Female Steroid Profiles 51 M. UEKI, M. OKANO, A. IKEKITA, T. HIRUMA, M. SATO: Epiandrosterone Glucuronide as a Sign to Indicate Natural Hormone Doping 71 R. KAZLAUSKAS: Effects of Dehydroepiandrosterone on Urinary Steroids 83 R. GRUCZA, K. CHROSTOWSKI, D. KWIATKOWSKA, A. POKRYWKA, A. CHAJEWSKI: Changes of Steroid Profiles in Male and Female Athletes 91

3 Ill Sample Preparation H. GEYER, W. SCHANZER, U. MARECK-ENGELKE, E. NOLTEERNSTING, G. OPFERMANN: Screening Procedure for Anabolic Steroids - The Control of the Hydrolysis with Deuterated Androsterone Glucuronide and Studies with Direct Hydrolysis 99 R. DE LA TORRE, X. DE LA TORRE, A. ARTOLA, S. CORCIONE, S. DICENTA, J. SEGURA: Hydrolyses Procedures in Sample Preparation. Esterase Activity Associated to Helix Pomatia Glucuronidase Preparations 103 M. MACHNIK, P. DELAHAUT, S. HORNING, W. SCHANZER: Preparation of Immuno Affinity Columns 121 G. SIGMUND, S. HORNING, I. SEINSCH, W. SCHANZER, M. DONIKE: Confirmation of Clenbuterol - Sample Preparation and HRMS 129 IV Techniques of MS Detection S. HORNING, H. GEYER, A. GOTZMANN, W. SCHANZER: Detection of Exogenous Steroids by l^cv 12c Analysis 135 E. NOLTEERNSTING, W. SCHANZER: Performance Test of the new Mass Selective Detector HP 5973 in Dope Analysis of Anabolic Androgenic Steroids 149 D. THIEME, J. GROSSE, R.K. MUELLER, A. WAHL: Interpretation of High Resolution and Tandem MS Data: Two Relevant Cases of Anabolic Steroid Metabolites at pg Level in Blood and Urine 157 J. MUNOZ-GUERRA, D. CARRERAS, C. SORIANO, C. RODRIGUEZ, A.F. RODRIGUEZ: Use of Ion Trap (GC-MS-MS) Mass Spectrometry for Detection and Confirmation of Anabolic Substances at Low Concentration Levels in Doping Analysis 169 M.A. POPOT, P. GARCIA, Y. BONNAIRE: Screening and Confirmation Method for the Detection of Synthetic Corticosteroids in Human Urine 185

4 V Peptide Hormones S. RENDIC, S. PICKETT, B. BROMLEY: Human Recombinant Erythropoietin (rhepo) - Physiology and Biochemistry 191 A. BREIDBACH, W. SCHANZER: Erythropoietin with Nichols Institute Diagnostics Chemiluminescence Immunoassay for Erythropoietin after Gelfiltration 209 S.M YANG, X.B WANG, Z.L FANG, SH. WANG, Y. QIN, M.Q YI, H. GAO, B.H XU, L. ZHOU, F.X ZENG, J.J GUO, ZH. Y YANG, J. DENG, M.H XI, Z.Y YANG, CH.J ZHANG: Observation on Hematology and Biochemistry of Six Chinese Following rhepo Administration 215 K. CHROSTOWSKI, R. GRUCZA: Diagnostic Validity of HDL-C, CK and LH Measured in Blood Serum for Discrimination of AAS Users during 'on' and 'off Cycle in Body Builders 223 VI Stimulating Substances A. TSOUTSOULOVA-DRAGANOVA, N. HALATCHEVA, D. KAROVA: Metabolism of Benzphetamine and Clobenzorex in Human Urine 231 D. DEBOER, M.L.D. DEGRAAF, P. GORTER, R.A.A. MAES: The Implementation of the Detection of the Abuse of 3,4-methylenedioxymethamphetamine and Analogues in Doping Control Screening Procedures 249 W. SCHANZER, A. GOTZMANN: The Cologne Protocol to Follow-up Positive Caffeine Cases 259 C. SCHWEIZER, C. CARDIS, M. SAUGY, L. RlVIER: Nicotine in Sport: Use or Abuse 269 M. UEKI, A. IKEKITA. M. OKANO, T. HIRUMA: Bromantan: - Japanese Experience V.F. SIZOI, S.L. BOLOTOV, V.A. SEMENOV: Study of Bromantan Metabolites Structure 287

5 VII New Aspects H.GEYER, P. BERSCHICK, U. MARECK-ENGELKE, W. SCHANZER: DNA Typing for the Confirmation of Manipulation in Dope Control: A Casework 301 M.I. MAGUREGUI, M.B. BARROSO, R.M. ALONSO, R.M. JIMENEZ: Capillary Electrophoresis (CE) for the Screening and Quantitative Determination of some Diuretics and fl-blockers 303 W. SCHANZER, G. SIGMUND-. Excretion Studies with Dextropropoxyphene 305 XU YOUXUAN, WU MOUTIAN, SHEN Li, WU YUN, WANG SHAN, CUI KAIRONG, ZHANG CHANGJIU: Studies on the Analysis of Dextropropoxyphene and its Metabolite in Human Urine 311 v.p. URALETS, P.A. GILLETTE, R.K. LATVEN: Fruitful Application of Steroid Methodology for Drug of Abuse Testing: Comprehensive Benzodiazepine Screening and Confirmation 317 S. HORNING, W. SCHANZER, B. SAMPLE: HRMS Analyses Performed at the 1996 Summer Olympic Games 329