Management and Disposal of Qiagen Materials

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Damian Ward
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Management and Disposal of Qiagen Materials Recently we have had a few incidences involving the use of bleach with a variety of chemical materials in Qiagen kits.

1 Management and Disposal of Qiagen Materials Recently we have had a few incidences involving the use of bleach with a variety of chemical materials in Qiagen kits. Most of the chemicals in these kits are not compatible with bleach and should be treated as chemical waste. When working with chemicals, always wear a suitable lab coat, disposable gloves, and protective goggles. For more information, please consult the appropriate safety data sheet as well as the Qiagen kit handbook ( DO NOT add bleach or acidic solution directly to the sample-preparation waste. Some of the kits contain guanidine hydrochloride, which can form highly reactive compounds when combined with bleach. Please refer to Table 1 as guide on how to management and dispose of Qiagen materials. Table 1: Guide on proper disposal of Qiagen Materials Reagent Name Chemical Components Management/Disposal Buffer AE Buffer AL Guanidinium 25-50% Buffer ALO Sodium Dodecyl % Buffer AP1 Sodium Dodecyl %

2 Buffer AP2 Acetic Buffer AP3/E Guanidinium 50- Buffer APP Zinc % Buffer ATL Sodium Dodecyl % Buffer AW Buffer AW1 Guanidinium 50- Buffer AW2 Sodium 0.1% Non-Hazardous Salt 99.9% Buffer BB Cetrimonium %

3 Buffer EB Buffer EC Buffer ETR Buffer EX Reaction Buffer Buffer N3 Buffer P1 Guanidinium 25-50% Acetic 10-25% Buffer P3 Acetic 10-25% Buffer PE Buffer PB Guanidinium 25-50% 25-50% Buffer PNI Guanidinium 25-50% 25-50%

4 Buffer QBT 10-25% Buffer QC 10-25% Buffer QG Guanidine 50- Buffer QLE Buffer QLL Buffer QLW Dimethyl-N-Lauryl-N-(3-Sulfopropyl)- Ammonium % Guanidinium % Guanidine % Buffer QN 10-25%

5 Buffer QS 10-25% Buffer QX1 Sodium 50- Buffer RDD Buffer RNAlater Buffer RLC Guanidinium 50- Buffer RLT Guanidine 25-50% Buffer RPE (concentrate) Buffer RT Buffer RW % Hydrogen % Guanidine % %

6 Buffer RWT Guanidine 25-50% Buffer S3 Acetic % dntp Mix Effectene Transfection Buffer Enhancer Exonuclease Solvent (Buffer) gdna Wipeout Buffer % GelPilot Loading Dye % LyseBlue

7 Lysozyme Multiplex PCR Master Mix 2x Omniscript Reverse Transcriptase OneStep Enzyme Mix OneStep RT-PCR Buffer % ph Indicator I Cresol <1% Proteinase K <1% Qiagen Resin QIAEX II Suspension Sodium 50-

8 QIAzol Lysis Reagent 25-50% Guanidine 10-25% Q-Solution 5x QuantiFast SYBR Green PCR Master Mix QuantiScript Reverse Transcriptase QuantiScript RT Buffer 5x QuantiTect RT Mix % 2-amino-2-(hydroxymethyl)propane-1, % Non-Hazardous Proprietary RNase A Solution % RT Primer Mix SuperFect Transfection Reagent SYBR Green RT-PCR Master Mix Sodium 0.27% Tris

9 **Can be drain disposed but the maximum combined total volume that can be discharged is 100 grams of solute per laboratory per day. After drain disposal, please flush with at least fold excess of water to thoroughly rinse out the sink and sink trap, and to dilute the waste. Note: The information in the table above only applies to the reagents when they are not mixed with any other chemicals. Please contact your EHS Safety Specialist for proper management/disposal of any Qiagen reagents not listed above.

Material Safety Data Sheet

Material Safety Data Sheet 1/5 1 PRODUCT AND COMPANY IDENTIFICATION Product Name Product Code FSQ-301, 301S SUPPLIER Name Address TOYOBO Co., Ltd. 2-8 Dojima Hama 2-chome, Kita-Ku OSAKA 530-8230 JAPAN Department Life Science Department