APPLICATION OF RADIOIMMUNOASSAY (RIA) FOR THE DETERMINATION OF RESIDUES OF ANABOLIC SEX HORMONES 1
|
|
- Allan Lester
- 6 years ago
- Views:
Transcription
1 APPLICATION OF RADIOIMMUNOASSAY (RIA) FOR THE DETERMINATION OF RESIDUES OF ANABOLIC SEX HORMONES 1 B. Hoffmann and C. Blietz Institut f~r Veterin~rmedizin des Bundesgesundheitsamtes (Robert yon Ostertag-Institut), D 1OOO Berlin 3 3 Summary Based on the basic principles of radioimmunoassay (RIA), it can be expected that by using this method, residues of anabolic sex hormones in animal tissues can be quantitated in the ng/g to pg/g range with adequate reliability. This was demonstrated for various endogenous and exogenous steroids as well as for stilbeneestrogens. Using the RIA for DES as a regulatory method allowed the successful control of the misuse of this compound in animal production, especially by examination of excreta. Also, for muscular tissue, an interlaboratory validaion study yielded acceptable results. The need for confirmatory methods is stressed. (Key Words: Radioimmunoassay, Residue Analysis, Anabolic Sex Hormones.) I ntroduction Radioimmunoassay (RIA) techniques for the determination of compounds with a low molecular weight, like steroid hormones, have been available for the past decade. Today, millions of assays are performed every year in research and clinical chemistry laboratories. It should be noted that many important and sometimes life saving therapeutic measures are based on data derived from RIA and, recently using a similar assay technique, enzyme-immunoassay (EIA). In general, measurements are performed on body fluids, predominantly blood plasma or serum. Presently, there is no other assay technique that can compete with RIA relative to reliability (i.e., specificity, precision, accuracy, reproducability), sensitivity (quantification in the fmol range) and assay speed. This paper will discuss to what extent these assay criteria are still applicable when Paper presented at the Symposium on "Metabolic Fate of Anabolic Substances in Food Animals" at the meeting of the Amer. Soc. of Anita. Sci., Raleigh, NC, USA, on July 29, RIA is applied to the determination of residues of anabolic sex hormones in tissues and excreta of food animals. Compounds, Metabolites and Required Assay Sensitivity In table 1 those anabolic compounds and combinations of compounds are listed that have been used most widely or are still in use for fattening of animals in various countries and continents. A distinction has to be made between the endogenous C18-, C19- and C21-steroids (estradiol-17/3, testosterone, progesterone) and the synthetic, nonendogenous steroids trenbolone acetate (TBA), methyltestosterone, metengestrol acetate (MGA) as well as the nonsteroidal exogenous estrogens diethylstilbestrol (DES), hexestrol (HEX), dienestrol (DIEN) and zeranol. Widely varying amounts of endogenous sex steroids occur naturally in food of animal origin (Hoffmann, 1981) in relation to sex, age and reproductive status of the animal. It has been shown (Henricks and Torrence, 1978; Hoffmann, 1978; Reid, 1980) that proper treatment of animals with endogenous sex steroids will not lead to hormone levels in the edible tissues, which at the time of slaughter would exceed the physiological range. Thus the necessity of setting tolerance levels from a toxicological point of view has not arisen. In contrast to this situation, xenobiotic compounds should only be used in animal production, if tolerance levels or an acceptable daily intake (ADD value have been established following toxicological evaluation of the compound. This also requires studying the metabolsim and pharmacokinetics in the target animal species, which will yield information as to whether residue analysis of the parent compound (drug administered) and(or) a metabolite is suitable for drug monitoring purposes. Thus the development of an RIA or any other JOURNAL OF ANIMAL SCIENCE, Vol. 57, No. 1, 1983
2 240 HOFFMANN AND BLIETZ TABLE I. COMPOSITION OF VARIOUS ANABOLIC PREPARATIONS Estrogenic Biological activity Application in Androgenic Gestagenic animal production 17#-estradiol 17#-estradiol Estradiol benzoate Estradiol benzoate Estradiol monopalmitate 17#-estradiol Diethylstilbestrol (DES) DES DES Hexestrol Dienestroldiacetate Zeranol Zeranol Testosterone Testosteronepropionate Trenbolone acetate (TBA) Trenbolone acetate (TBA) Testosterone Methyltestosterone TBA Progesterone Progesterone Melengestrol acetate (MGA) Veal calf Steer, heifer, lamb Steer, heifer, lamb Poultry Veal calf, steer, heifer Heifer, cow Heifer Steer, heifer, lamb, veal calf Steer, heifer Pig Steer, heifer, lamb Poultry Steer, heifer, lamb, veal calf Steer, heifer, lamb, veal calf nonradiometric method should be based on this knowledge. By neglecting this proper identification of parent compounds and metabolites, errors were made in the past. For example, the antiserum, and hence RIA, available for the determination of TBA and its free form, 17~-trenbolone (Hoffmann and Oettel, 1976), only cross-reacts 15 to 20% with 17c~-trenbolone (Schopper and Hoffmann, 1981), which later was identified as the major extractable metabolite of TBA in liver and kidney of cattle, where highest residue levels are found (Pottier et al., 1980; Schopper, 1981). In no case is RIA or any other method that is based on the extraction of tissues with an organic solvent able to measure the covalently bound nonextractable residues, which by far exceed the extractable ones in the case of TBA (Ryan and Hoffmann, 1978; Schopper, 1981). Regardless of the type of residue to be measured, the reliability of a method has to be established. In this respect, most of the assay problems arising are a function of the required level of sensitivity. As outlined in table 2, an adequate method for monitoring the treatment with endogenous steroids should allow analysis of the physiological tissue concentrations, while methods for residue analysis of xenobiotics should allow a safe estimate of the tolerance levels given. If the use of a pharmacologi- TABLE 2. METHODS FOR RESIDUE ANALYSIS OF ANABOLIC AGENTS: REQUIRED LEVELS OF SENSITIVITY Measurement of: Endogenous steroids Nonendogenous, synthetic compounds (xenobiotics) allowed to be used as animal drugs Compounds prohibited, from use in food animals Required sensitivity Adequate to measure physiological concentrations Adequate to measure residues in one or several tissues based on the ADl-value a Highest sensitivity available aadi = acceptable daily intake.
3 RESIDUE ANALYSIS OF ANABOLIC SEX HORMONES 241 cally active compound in food animals is prohibited, as it is in the case of DES in most countries, the method used for residue analysis should have the highest sensitivity possible. Methods Available and Assay Specificity The extent to which RIA is available for the measurement of anabolic sex hormones in edible animal tissues is summarized in table 3. Most methods have been published in detail and a review particularly dealing with the reliability has been given elsewhere (Hoffmann, 1978). Thus, only some general remarks on assay specificity, perhaps the most important reliability criterion, will be given. When using RIA, specificity first relates to the quality of the antiserum or antibodies used. The extent to which the antibodies reflect the stereospecificity of the compounds to be measured, largely depends on the type of functional group used to couple the steroid or anabolic agent to a protein; which of course is a necessary step to induce antigenicity (Niswender and Midgley, 1970; Pdron and Caldwell, 1970). As is shown for 17~estradiol in figure 1, an antigen made by coupling this steroid through its 17~hydroxy-group to a protein yielded, after immunization of a rabbit, an antiserum that showed almost identical cross-reactions with 17/3-estradiol, estrone and 17a-estradiol. This type of cross-reactivity was expected, because the only structural difference between these three estrogens is their functional group at carbon 17 (17~-hydroxy; 17-keto; 17r hydroxy group), which was hindered due to the coupling (antiserum I). When using a different approach by not interfering with the structure at carbon 17 and coupling 17~-estradiol to the protein through a keto-group introduced at carbon 6, antisera can be obtained, which exhibit a distinctly lowered cross-reactivity with estrone and 17cx-estradiol (antiserum II). Consequently, it is possible to obtain highly specific antisera and even structure identification has been performed by using various antisera (Kuss et al., 1973). However, assay interference due to the presence of impurities may occur. This phenomenon relates to the binding kinetics of the antibodies. Apparently the problem is negatively correlated to the percentage of high affinity binding sites in the antiserum. Nevertheless, it has been experienced during the past decade, that high quality antisera also require a certain "clean up" procedure of the sample. This procedure may vary depending on the type of tissue examined and the demands on the assay sensitivity, which directly relates to the distribution of the drug within the animal. In general residues of anabolic sex hormones are lowest in muscular tissue and highest in the excreta (Hoffmann and Karg, 1976; Karg and Vogt, 1981). For example, a similar degree of certainty in detecting a treatment with DES is obtained when the assay applied is able to detect concentrations around.02 ng/g in muscle or 5 ng/g in feces (table 4). Also, determination of DES by R1A in concentrations of less than.1 ng/g, requires quite a lengthy "clean up" of the extract (Hoffmann and Laschiitza, 1980; Vogt, 1980), while following a simple extraction, the impurities can more or less be diluted out, when DES ranges above 5 ng/g feces (Agthe, 1980). It should be noted, that in both assays the absolute amounts of DES finally quantified TABLE 3. RAD1OIMMUNOASSAY METHODS AVAILABLE FOR MEASUREMENT OF RESIDUES OF ANABOLIC AGENTS Tissue Hormone Muscle Liver Kidney Fat Reference Estradiol-17/ Estrone Testosterone Progesterone (+) (+) (+) Trenbolone DES Hexestrol/dienestrol + + Zeranol In development + + For survey of literature see Hoffmann (1978) Laschiitza and Hoffmann (1979) Hoffmann and Laschiitza (1980) Harwood et al. (1980) Dixon and Heitzman (1981)
4 242 HOFFMANN AND BLIETZ 100- t"='~="~."~ I~ z z50- X 0 \, b, ', %% % 9 II M I I I 1 ~ I I t I I ~ ~ 5 STEROID, ng STEROID, ng 9 estrodiol-l"/b; 0 estrone ; = estrodiol-l"/c~ 13 non phenotic steroids I.s-Bs II oh CMO-BSA 17fl- estradioi fl-estradio[- 6 - corboxy- hemisuccinate- 8SA methyl- oxime- BSA Figure 1. Cross-reactivity of antisera directed against 170-estradiol-17-hemisuccinate-BSA (I) and 17~-estradiol-6-carboxymethyl-oxime-BSA (1I) (see Hoffmann, 1978). TABLE 4. DETERMINATION OF DIETHYLSTILBESTROL: POSTULATED REQUIRED METHOD SENSITIVITY IN RELATION TO THE BIOLOGICAL MATRIX EXAMINED a Biological matrix - z Required lower levels of sensitivity, ng/g Muscle ~.02 Liver-kidney ~ Urine ~2.0 Feces ~5.O ahoffmann (1980), by RIA are in the same order of magnitude; in general the calibration curve ranges from 10 to 200 pg per tube. Due to this very high final assay sensitivity, the sample size can be limited to a maximum of 1 g tissue and hence a good practical method is still obtained even in the case of longer extraction procedures. Special Experience with the RIA for the Determination of DES Approaches to determine DES by RIA date back to the early seventies. However, in spite of
5 RESIDUE ANALYSIS OF ANABOLIC SEX HORMONES 243 the need to have an assay available as soon as possible, as was expressed by various regulatory agencies, and the money put into methoddevelopment, no substantial progress was made until 1978; at that time amethod was published anonymously in the Regulations of Meat Inspection Act 2 (Implementing Regulation A) of the Federal Republic of Germany prior to publication in a scientific journal. The fully validated method was not published before 1980 (Hoffmann and Laschiitza, 1980). Retrospectively, it can be said that so far the most successful approach to obtain antisera against DES proved to be the use of DEScarboxypropylether (figure 2), coupled to bovine serum albumin, as an antigen (Rombauts et al., 1973; Laschiitza, 1980). By using the same type of derivative as an antigen, antisera have also been obtained for the determination of hexestrol (Harwood et al., 1980) and dienestrol (A. Jouquey, unpublished data). Since 1981, 3H-DES (tracer used for RIA) and antisera against DES and hexestroi can be purchased in the form of a kit 3. As shown in figures 3 and 4 and table 5, the two available antisera differ markedly in their cross-reactivity between the stilbene-estrogens, while there is no difference in their existing cross-reactivity with respect to the other compounds tested. Obviously, both antisera exhibit a 100% cross-reactivity with the stilbene (DES or hexestrol) used for antigen synthesis. However, while antiserum 254 only cross-reacts 16.5 and 6.4% with hexestrol and dienestrol, respectively, antiserum 6139 cross-reacts 40 to 50% with DES and 16 to 25% with dienestrol (table 5). Hence, it was suggested in a method proposal submitted to the Commission of the European Communities 4 to use antiserum AS 254 for the specific determination of DES and antiserum AS 6139 for analysis of hexestrol and group analysis of stilbene-estrogens. How- 2Bundesgesetzblatt (Federal Register), Part I, Z 1997 A. Neufassung der Ausf(ihrungsbestimmungen A fiber die Untersuchung und gesundheitspolizeiliche Behandlung der Schlachttiere und des Fleisches bei Schlachtungen im Inland. Bonn, Hoechst AG, Pharma Forschung, D-6230 Frankfurt 80 (Main). 4Commission of the European Communities; Working Document 4694/VI/81-EN, File No Radioimmunoassay (RIA) methods for the measurement of residues of the stilbene derivatives, diethylstilbestrol, hexestrol and dienescrol in tissues and fluids of farm animals. HO ~ " " Figure 2. DES-carboxypropylether-bovine serum albumin. I. Io 00ts o~. Ilntx o(~,~ o1(. sc on '~176 -" a _~ I*1 m ~ 1 1o z 1o ] io; lo 5 StjLSs / st(aojo. P9 Figure 3. Cross-reaction of antiserum directed against DES-carboxypropylether-BSA (AS 254). Assay conditions: dilution (1:80,000), incubation (1 h at 37 C), separation (charcoal.2% in detain, water). Substances tested: HEX (hexestrol), DES (diethylstilbestrod, DIEN (dienestrol), P4 (progesterone), E: (estradio1-173), C-on (corticosterone) and Det (detergent). m,tx 0 lacg 9 oes o [z 9 aetn A C o~ (. os :,o~p~ l '"I "' 8 : 9 t... :. io I io z io 3 ~a ~ io s STaLSEN(/ sr(~o~o, pr Figure 4. Cross-reaction of antiserum directed against HEX-carboxypropylether-BSA (AS 6139; Harwood et al., 1981). Assay conditions: dilution (1: 24,000), incubation (1 h at 37 C), separation (charcoal.2% in detain, water). DES (diethylstilbestrol), DIEN (dienestrol), P4 (progesterone), E 2 (estradiol-17~), C-on (corticosterone) and Det (detergent).
6 244 HOFFMANN AND BLIETZ ever, contrary to the RIA for the determination of DES and hexestrol, the assay for group analysis of stilbene estrogens has to be further validated and optimized. In this respect it should be noted that massive contaminations with detergent and probably other impurities interfere with the assay (figures 3 and 4). Also the absolute binding as well as the amount of cross-reactivity exhibit some variations, depending on the time of incubation (table 5). As indicated above, the need for an adequate method for the determination of DES-residues in edible animal tissues was expressed by various regulatory agencies, because the use of DES in animal production has been prohibited or strictly regulated in most countries. In 1981, the Commission of the European Communities banned the use of DES and other stilbene estrogens in all member states. Thus, in theory, no residues of DES can be tolerated and the methods to monitor for DES should enable implementation of this philosophy or regulation. Nevertheless, DES has been used illegally on a large scale in beef and veal production. As a result, in some European countries DES courd be detected even in baby food. As the major source of contamination, the high residue concentrations remaining at the site of application over extended periods of time have to be considered (Hoffmann and Karg, 1976; Karg and Vogt, 1981). This was demonstrated in our own recent experiments, which were also performed in order to provide positive and negative control samples for the regulatory agencies within the Federal Republic of Germany. The method outlined in the Regulations of Meat Inspection Act (Implementing Regulation A) of the Federal Republic of Germany (see above), requires an analysis of positive and negative tissue samples with each assay, and the field sample collected can only be estimated as DES positive if there is a significant difference between the negative control assay. As shown in table 6, DES concentrations varied between.06 and 17 ng/g at the injection site (M. triceps brachii), while residue concentrations in the other muscle sites examined were around 70 pg/g, just above the level of detection. However, previous results (Hoffmann and Laschiitza, 1980; Harwood et al., 1980; Karg and Vogt, 1981) had distinctly higher residue levels in liver and kidney, particularly if the conjugated residues were estimated. As described elsewhere (Hoffmann and Oettel, 1976), quantification of conjugated residues is performed by introducing enzymatic hydrolysis of the sample prior to the initial ether extraction. From our present experiences it can be delineated that in order to achieve adequate consumer protection, residue analysis should detect the presence of such a depot at the injection site. Because the probability of sampling an injection site when examining muscular tissue is rather low, the most reliable information can be obtained by examining excreta (urine, feces, bile). This approach has proven to be rather successful in the Federal Republic of Germany, particularly because the TABLE 5. ABSOLUTE BINDING AND CROSS-REACTION OF ANTISERA AGAINST STILBENE-ESTROGENS IN RELATION TO THE TIME OF INCUBATION AND ANTISERUM DILUTION Final Time of Absolute a assay incubation, binding, Cross-reaction (%) Antiserum dilution h % DES Hexestrol Dienestrol :80, :128, :24, :48, a40 pg 3 H-DES ~.0167 Ci.
7 RESIDUE ANALYSIS OF ANABOLIC SEX HORMONES 245 TABLE 6. FREE AND CONJUGATED DIETHYLSTILBESTROL (DES) IN TISSUES OF A VEAL CALF 7 DAYS AFTER INTRAMUSCULAR INJECTION OF 200 MG DES IN OILY SOLUTION Tissue Injection site a DES Muscle Liver Kidney "Edge" Range Free Conjugated alnjection given into the M. triceps brachii. About 500 g of tissue were removed at slaughter and segments of 10 to 20 g were examined individually. examination of feces allowed the control of the live animal already at the production unit (Agthe et al., 1979; Agthe, 1980). The effectiveness becomes apparent from unofficial statistics from various Veterinary Control Laboratories that show that within 1 yr following the introduction of this method, the number of veal calves identified as treated with DES dropped from approximately 40% to less than 1.0% (Hoffmann, 1982). Due to the export-import business of carcasses, tissue methods also have to be available and applied. As is shown for muscle in table 7, 2 yr after introduction of the RtA for DES, an inter-laboratory validation yielded quite acceptable results. Finally, it should be stressed that confirmatory methods based on a different technique of TABLE 7. RESULTS (JULY, 1981), INTER-LABORATORY VALIDATION: RADIOIMMUNOASSAY FOR DES IN MUSCULAR TISSUE OF A CALF INJECTED (INTRAMUSCULAR) WITH 150 MG DES- DIPROPRIONATE AS A CRYSTAL SUSPENSION 3 WEEKS BEFORE SLAUGHTER Laboratory No. a pg/gb CV c, % d a Number of duphcates or triplicates. bmean SE. Ccoefficient of variation. dmodified extraction procedure. measurement will be necessary. Problems in this respect particularly concern the determination of the low residue concentrations observed in muscular tissue outside the injection site. However, due to recent progress with gas chromatography-mass-spectrometry (Bergner- Lang and K~ichele, 1981) this gap should soon be closed. Literature Cited Agthe, O Die anwendung des radioimmunoassay f(ir Di~ithylstilb6strol auf kotproben von mastk/ilbern. Arch. f. Lebensmittelhyg. 31 : 102. Agthe, O., D. de Frenne and C. M. Sperveslage her die verbreitung der anwendung yon 6strogenen in der k/ilbermast im raum weser eros. Arch. f. Lebensmittelhyg. 30:67. Bergner-Lang, B. and M. K~chele Anabotica in kalbfleisch. Nachweis und bestimmung, befunde und beobachtungen. Deutsche Lebensmittel- Rundschau 77: 305. Dixon, S. N. and R. J. Heitzman Residues of exogenous anabolic agents in beef cattle and sheep, in: Anabolic agents in beef and veal production, pp Commission of the European Communities. Harwood, D. J., R. J. Heitzman and A. Jouquey A radioimmunoassay method for the measurement of residues of the anabolic agent, hexoestrol, in tissues of cattle and sheep. J. Vet. Pharmacol. Therap. 3:245. Henricks, D. M. and A. K. Torrence Endogenous estradiol-173 in bovine tissues. J. Assoc. Official Anal. Chem. 61:1280. Hoffmann, B Use of radioimmunoassay (RIA) for monitoring hormonal residues in edible animal products. J. Assoc. Official Anal. Chem. 61:1263. Hoffmann, B Analytischer stand des 6strogennachweises. In: Proc. 21. Arbeitstagung des Arbeitsgebietes Lebensmittel-hygiene. pp DVG, Frankfurter Str. 87, D-6300 Gie~en. Hoffmann, B Levels of endogenous anabolic sex hormones in farm animals. In: Anabolic Agents in Beef and Veal Production. pp Commission of the European Communities.
8 246 HOFFMANN AND BLIETZ Hoffmann, B Derzeitiger stand des stilbennachweises und gesetzliche grundlagen. Fleischwirtschaft 62:95. Hoffmann, B. and H. Karg Metabolic fate of anabolic agents in treated animals and residue levels in their meat. In: F. Coulston and F. Corte (Ed.). Environmental Quality and Safety. Suppl. Vol. 5. Anabolic Agents in Animal Production. pp Georg Thieme Verlag, Stuttgart, Germany. Hoffmann, B. and W. Lasch~itza Entwicklung eines radioimmunotests zur bestimmung von di~thylstilbisstrol im blutplasma and e~baren geweben vom rind. Arch. f. Lebensmittelhyg. 31:105. Hoffmann, B. and G. Oettel Radioimmunoassay for free and conjugated trienbolone and for trienbolone acetate in bovine tissue and plasma samples. Steroids 27: 509. Karg, H. and K. Vogt Residues of diethylstilboestrol (DES) in veal calves. In: Anabolic Agents in Beef and Veal Production. pp Commission of the European Communities. Kuss, E., R. Goebel and H. Enderle Influence of oxo-, and/or hydroxy-groups at C-16/C-17 of estrogens and affinity to anti-estrone-, antiestradiol-17~- and anti-estradiol-17~-antisera. Hoppe-Seyler's Z. Physiol. Chem. 354: 347. Laschiitza, W Entwicklung eines radioimmunologischen verfahrens zur bestimmung der anabol wirksamen substanz di~ithylstilb6strol (DES) im blutplasma und in ea3baren geweben vom rind. Dissertation Rer. Nat. Miinchen, Technische Universit/it Miinchen. LaschiJtza, W. and B. Hoffmann Radioimmunoassay of diethylstilboestrol (DES) in muscular tissue of calves. Acta Endocrinol. Suppl. 225:98. Niswender, G. D. and A. R. Midlgey, Jr Haptenradioimmunoassay for steroid hormons. In: F. G, P~ron and B. V. Caldwell (Ed.). Immunological Methods in Steroid Determination. pp Appleton Century Crofts, New York. P~ron, F. G. and B. V. Caldewell Immunologic Methods in Steroid Determination. Appleton Century Crofts, New York. Pottier, J., D. Coussediere and R. J. Heitzman Levels of 17a and 17~3-trienbolone in tissues and bile of heifers implanted with trenbolone acetate. J. Anita. Sci. 51(Suppl. 1):316. Reid, J.F.S Significance of natural estrogenimplanted beef to human health. In: The Use, Residues and Toxicology of Growth Promoters. pp An Foras Taluntais, Dublin. Rombauts, P., A. Pierdet and A, Jouquey Preparation et propri6ties d'immun-s~rum antidi~tylstilboestrol. C. R. Acad. Sci., Paris. S~r. D. 277:1921. Ryan, J. J. and 13. Hoffmann Trenbolone Acetate (TBA): Experiences with bound residues in cattle tissue. J. Assoc. Official Anal. Chem. 61:1274. Schopper, D Messungen yon rijckst~inden yon ostradiol- 17~3, testosteron und progesteron im plasma und teilweise im urin yon mastrindern nach anabolikabehandlung sowie untersuchungen zum stoffwechsel yon trenbolonacetat. Dissertation Med. Vet., MiJnchen, Ludwig-Maximilians- Universit~/t. Schopper, D. and 13. Hoffmann Identifizierung yon 17c~- Trenbolon als hauptausscheidungsprodukt des trenbolonacetatstoffwechsels beim Kalb und sich daraus ergebende konsequenzen f/jr die riickstandsanalytik. Arch. f. Lebensmittelhyg. 32.:141. Vogt, K Vereinfachtes extrakhons-und reiniguhgsverfahren fur die radioimmunologlsche bestimmung yon DES in fleisch, leber und neire. Arch. f. Lebensmittelhyg. 31:138.
THE ABSORPTION, DISTRIBUTION AND EXCRETION OF ANABOLIC AGENTS 1
THE ABSORPTION, DISTRIBUTION AND EXCRETION OF ANABOLIC AGENTS 1 R. J. Heitzman ARC Institute for Research on Animal Diseases, Compton, Newbury, Berkshire, RG16 ONN, United Kingdom Summary The metabolic
More informationSCREENING OF TRENBOLONE-17β IN MILK SAMPLES AFTER APPLICATION OF TRENBOLONE ACETATE TO A CULL COW
SCREENING OF TRENBOLONE-17β IN MILK SAMPLES AFTER APPLICATION OF TRENBOLONE ACETATE TO A CULL COW Iris G. Lange, Andreas Daxenberger, Heinrich H. D. Meyer Institut für Physiologie, Forschungszentrum für
More informationSIMPLE, EFFICIENT AND SIMULTANEOUS DETERMINATION OF ANABOLIC STEROIDS IN HUMAN URINE
SIMPLE, EFFICIENT AND SIMULTANEOUS DETERMINATION OF ANABOLIC STEROIDS IN HUMAN URINE Pages with reference to book, From 216 To 218 S.J. Khurshid, M. Riaz, S. Ahmed ( Nuclear Chemistry Division, Pakistan
More informationGROWTH PROMOTERS Chapter 5
GROWTH PROMOTERS Chapter 69 Growth Promoters b-agonists ANTIBODIES TRACER SPECIFICITY IMMUNOGEN Unit* Cat. No. Tracer Description Unit Cat. No. CIMATEROL Cimaterol-BSA 1ml PAS9848 Cimaterol-HRP 0.ml HRP9410
More informationWADA Technical Document TD2014EAAS. Endogenous Anabolic Androgenic Steroids Measurement and Reporting
Endogenous Anabolic Androgenic Steroids Measurement and Reporting 1.0 Introduction The purpose of this Technical is to harmonize the approaches to the measurement and reporting of endogenous anabolic androgenic
More informationNational Institute for Public Health and the Environment Annual CRL workshop 22 October Update on natural Hormone studies
2008 Annual CRL workshop 22 October 2008 Update on natural Hormone studies Natural hormone studies: update Possible approaches - C12/C13 ratio: can result in proof of abuse - Determination of intact esters
More informationPresence and metabolism of natural steroids in meat-producing animals: current knowledge and potential strategies for detecting their abuse
Presence and metabolism of natural steroids in meat-producing animals: current knowledge and potential strategies for detecting their abuse James Scarth, HFL, UK October 2007 Overview of presentation Context
More informationAndrogen Measurements
Chapter 5 / Androgen Measurements 63 Androgen Measurements Methods, Interpretation, and Limitations 5 Frank Z. Stanczyk SUMMARY Androgens are measured primarily in serum by direct and indirect (with one
More informationWADA Technical Document TD2018MRPL
MINIMUM REQUIRED PERFORMANCE LEVELS FOR DETECTION AND IDENTIFICATION OF NON-THRESHOLD SUBSTANCES In order to ensure that all WADA-accredited Laboratories can report the presence of Prohibited Substances,
More informationDevelopment of a Clinical Research Method for the Measurement of Testosterone. Dihydrotestosterone
Development of a Clinical Research Method for the Measurement of Testosterone and Dihydrotestosterone Martijn van Faassen, Maarten van den Noort and Ido Kima University Medical Center, Groningen, Netherlands
More informationSimultaneous Determination of a Panel of 22 Steroids in Urine and Serum by SPE and LC-MS/MS
Simultaneous Determination of a Panel of 22 Steroids in Urine and Serum by SPE and LC-MS/MS UCT Part Numbers: CUQAX22Z Clean-Up C8+QAX, 2mg/1mL BETA-GLUC- ml Beta-Glucuronidase Enzyme, liquid form SLAQID21-3UM
More informationCOMMUNITY REFERENCE LABORATORIES IN THE FIELD OF VETERINARY PUBLIC HEALTH WITHIN THE EUROPEAN UNION
COMMUNITY REFERENCE LABORATORIES IN THE FIELD OF VETERINARY PUBLIC HEALTH WITHIN THE EUROPEAN UNION CRL for residues RIVM-ARO at Bilthoven, NL --------------------------------- Annual Report January 2003
More informationMonitoring Hormone Therapy Mark Newman, M.S. President of Precision Analytical Inc.
Mr Mark Newman Monitoring Hormone Therapy Mark Newman, M.S. President of Precision Analytical Inc. 2 Avoiding Pitfalls in (B)HRT Monitoring and Picking the Right Lab Test Objectives: Outline how expected
More informationExtend the time window for the detection of low level anabolic androgenic steroids and their metabolites
Application Note Doping Control Extend the time window for the detection of low level anabolic androgenic steroids and their metabolites Steroid profiling, screening, and confirmatory analysis using the
More informationRECENT ADVANCES IN DOPING ANALYSIS (2)
M. DONIKE H. GEYER A. GOTZMANN U. MARECK-ENGELKE (EDITORS) RECENT ADVANCES IN DOPING ANALYSIS (2) Proceedings of the 12th Cologne Workshop on Dope Analysis 10th to 15th April 1994 SPORT und BUCH StrauB
More informationMSD 96-Well MULTI-ARRAY and MULTI-SPOT Human Granulocyte Colony Stimulating Factor (hg-csf) Ultrasensitive Assay
MSD 96-Well MULTI-ARRAY and MULTI-SPOT Human Granulocyte Colony Stimulating Factor (hg-csf) Ultrasensitive Assay Summary This assay measures Human Granulocyte Colony Stimulating Factor (G-CSF) in a 96-well
More informationLevels of the natural hormones 17β-oestradiol and testosterone in serum of cattle: results from population studies in Poland
DE G DE GRUYTER OPEN J Vet Res 60, 461-466, 2016 DOI: 10.1515/jvetres-2016-0055 Levels of the natural hormones 17β-oestradiol and testosterone in serum of cattle: results from population studies in Poland
More informationLIFETIME FITNESS HEALTHY NUTRITION. UNIT 2 Lesson 14 FLEXIBILITY LEAN BODY COMPOSITION
LIFETIME FITNESS HEALTHY NUTRITION MUSCULAR STRENGTH AEROBIC ENDURANCE UNIT 2 Lesson 14 FLEXIBILITY LEAN BODY COMPOSITION MUSCULAR ENDURANCE Created by Derek G. Becher B.P.E., B. Ed., AFLCA Resistance
More informationWADA Technical Document TD2016EAAS. Endogenous Anabolic Androgenic Steroids Measurement and Reporting
Endogenous Anabolic Androgenic Steroids Measurement and Reporting Introduction The purpose of this Technical Document (TD) is to harmonize the approaches to the measurement and reporting of Endogenous
More informationViapath Innovation Academy 5 December 2014
Viapath Innovation Academy 5 December 2014 Interview with Dr Andrew Kicman of King s College London conducted by Robert Dunn of the Future Leaders in Innovation Group, Viapath. How did you choose your
More informationAnabolic sex hormones have been used as. Detection of Anabolic Residues in Misplaced Implantation Sites in Cattle
DAXENBERGER ET AL.: JOURNAL OF AOAC INTERNATIONAL VOL. 83, NO. 4, 2000 809 DRUGS, COSMETICS, FORENSIC SCIENCES Detection of Anabolic Residues in Misplaced Implantation Sites in Cattle DAXENBERGER ET AL.:
More informationClient: Ultimate Fitness Events - USA 600 Golden Gate St Lake Orion, MI Phone: (248) Identification: 104 DOB: Sex:
Laboratory Directors: Mark J. DeMeo, M.D.; Richard R. Wilber, M.D. Identification: 104 DOB: Sex: Account #: Requisition #: 131535 900007 Collected by: DR. SHAWN JENNINGS Accession #: 160622-01174 Collected:
More informationEVALUATION OF PROFICIENCY TESTING PROGRAM, SETTING PRIORITIES FOR THE FUTURE.
Click to edit Master title style EVALUATION OF PROFICIENCY TESTING PROGRAM, SETTING PRIORITIES FOR THE FUTURE. Saskia S. Sterk Henk A. Herbold, Marco H. Blokland October 2005. Introduction. One of the
More informationPros and Cons of Hormone Testing in Different Body Fluids with Different Routes of Hormone Delivery
Pros and Cons of Hormone Testing in Different Body Fluids with Different Routes of Hormone Delivery David T Zava, PhD ZRT laboratory A Guide to Steroid Hormone Testing in Different Body Fluids Following
More informationPRELIMINARY SCREENING FOR THE LEVELS OF TESTOSTERONE HORMONE IN THE MARKET MEAT IN TEHRAN
PRELIMINARY SCREENING FOR THE LEVELS OF TESTOSTERONE HORMONE IN THE MARKET MEAT IN TEHRAN M. R. Oveisi 1, B. Jannat 2, N. Sadeghi 1*, M. Hajimahmoodi 1 and M. Bagheri 1 1) Department of Food and Drug Control,
More informationHealth Products Regulatory Authority
1 NAME OF THE VETERINARY MEDICINAL PRODUCT Myodine 25 mg/ml solution for injection for dogs and cats 2 QUALITATIVE AND QUANTITATIVE COMPOSITION Each ml contains: Active substance: Nandrodine laurate 25
More informationPhysician Copy. Patient: SAMPLE PATIENT DOB: Sex: MRN: ACH24.1
Physician Copy Patient: SAMPLE PATIENT DOB: Sex: MRN: ACH24.1 ID: Steroidogenic Pathway At-A-Glance 0.60 1 0.10 RR : 0-0.90 RR : 0.30-4.20 RR : 0.60-2.50 0.10 RR : 0.16-0.65 1.48 RR : 1.20-6.10 2.47 RR
More informationFair Competition and Drug Control
Fair Competition and Drug Control Speaker: Dr. Yves Bonnaire General Manager, Laboratoire des Course Hippiques Dr Yves BONNAIRE Advisory Council LOGO PROHIBITED SUBSTANCES an update. PROHIBITED SUBSTANCES
More informationNow it is. easy to switch. CHS TM Steroid Profiling kit and software. Brochure not for distribution in the USA and Canada
Now it is easy to switch to Clinical MSMS CHS TM Steroid Profiling kit and software Brochure not for distribution in the USA and Canada 2 Meeting your needs for steroid assays PerkinElmer s new CHS MSMS
More informationThe Application of QuEChERS in the Extraction of Anabolic Steroids in Whole Blood
The Application of QuEChERS in the Extraction of Anabolic Steroids in Whole Blood UCT Part Numbers: ECQUUS1015CT- Enviro-Clean 15 ml centrifuge tube with 400 mg MgSO 4 and 100 mg NaCl CUMPS2CT - Enviro-Clean
More informationAll you need to know about anti-doping. Representatives from Hong Kong Anti-Doping Committee
All you need to know about anti-doping Representatives from Hong Kong Anti-Doping Committee Anti-Doping Rule Violations What is DOPING? The occurrence of one or more of the anti-doping rule violations
More informationAnabolics: the approach taken in the USA
Anabolics: the approach taken in the USA Tm Farber To cite this version: Tm Farber. Anabolics: the approach taken in the USA. Annales de Recherches Vétérinaires, INRA Editions, 1991, 22 (3), pp.295-298.
More informationGoing! Going! Gone! Your favorite slugger just hit a game winning homerun and you re
James Krein jvk2@uakron.edu Economic Essay on Something Curious The University of Akron September 8, 2006 Why Do Professional Athletes Take Steroids and Other Banned Substances? In this paper I will discuss
More informationAndrostenedione and testosterone concentrations in plasma and milk of the cow throughout pregnancy
Androstenedione and testosterone concentrations in plasma and milk of the cow throughout pregnancy Rosella Gaiani, F. Chiesa, M. Mattioli, G. Nannetti and Giovanna Galeati Istituto di Fisiologia Veterinaria
More informationWADA Technical Document TD2017NA
HARMONIZATION OF ANALYSIS AND REPORTING OF 19-NORSTEROIDS RELATED TO NANDROLONE 1.0 Introduction This document has been established to harmonize the Confirmation Procedure for the analysis and reporting
More informationToxicological aspects of anabolic hormone use in meat production
Toxicological aspects of anabolic hormone use in meat production Author: Dr. Francis J.C. Roe, DM, DSc, FRC Path. Date: 6th June, 1981 Contribution to: Anabolizzanti. Zootecnici e Salute Pubblica (A two
More informationLCMSMS Solutions For Steroid Analysis
LCMSMS Solutions For Steroid Analysis Dan Blake Manager, Clinical Applications For Research Use Only. Not for use in diagnostic procedures. RUO-MKT-18-1166-A Agenda Special considerations for steroid analysis
More informationThe ICL Insider. Lab Testing: Testosterone. In This Issue. The Debate
The ICL Insider February 2017 Volume 2, Issue 2 Lab Testing: Testosterone In This Issue Testosterone shashtilak@iclabs.ca Next Issue: Expected Release April 2017 The Debate Aging is accompanied by various
More informationIntroduction to the Doping Problem
Introduction to the Doping Problem Jordi Segura Director, WADA Accredited Antidoping Laboratory, IMIM-Hospital del Mar, Barcelona, Spain Doping and Society: towards the perfect human machine? ESOF, Barcelona
More informationAnabolics: the situation in Belgium
Anabolics: the situation in Belgium G MaghuinRogister, P Gaspar, M Vandenbroeck, P Schmitz, G Degand, G Pelzer, J Degraeve To cite this version: G MaghuinRogister, P Gaspar, M Vandenbroeck, P Schmitz,
More informationComplete. Male Hormones SAMPLE PATIENT. Patient: Order Number: Age: 47 Sex: M MRN:
Patient: Age: 47 Sex: M MRN: SAMPLE PATIENT Order Number: 81200334 Completed: September 20, 2007 Received: September 20, 2007 Collected: September 20, 2007 Complete Male Hormones Patient: SAMPLE PATIENT
More informationA Simplified Method for Extracting Androgens From Avian Egg Yolks
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln USGS Staff -- Published Research US Geological Survey 2009 A Simplified Method for Extracting Androgens From Avian Egg Yolks
More informationUpdates on Anti-doping and TUE Management in Paralympic Sport
International Paralympic Committee Updates on Anti-doping and TUE Management in Paralympic Sport Matthew Fedoruk, Ph.D. March 15, 2018 PyeongChang 2018 IPC Medical / Sports Science Committee Workshops
More informationab Testosterone ELISA Kit
Version 10 Last updated 29 January 2018 ab108666 Testosterone ELISA Kit A competitive immunoenzymatic assay for the quantitative measurement of Testosterone in serum, plasma and urine. This product is
More informationFrank Zanzuccki, Executive Director. See Summary below for explanation of exception to calendar requirement
LAW AND PUBLIC SAFETY NEW JERSEY RACING COMMISSION Horse Racing Anti-Recombinant Human EPO Antibody Testing Program Proposed New Rule: Authorized By: N.J.A.C. 13:70-14A.12 New Jersey Racing Commission,
More informationFemale testosterone level chart
Female testosterone level chart The Borg System is 100 % Female testosterone level chart Mar 23, 2015. Male, Female. Age: T Level (ng/dl):, Age: T Level (ng/dl):. 0-5 mo. 75-400, 0-5 mo. 20-80. 6 mos.-9
More informationFree testosterone levels during danazol therapy*
FERTILITY AND STERILITY Copyright 1983 The American Fertility Society Printed in U.8A. Free testosterone levels during danazol therapy* Bo Nilsson, M.D. t:j: Ragnar Stidergard, Ph.D. Mats-GQran Damber,
More informationUWW ANTI-DOPING PANEL DECISION. Case
UWW ANTI-DOPING PANEL DECISION Case United World Wrestling (UWW) v. Mr Husham Majeed Ali AL THAALEBI (Athlete GR wrestling member of the Iraqi national team) Panel: - Dr Daniel Wozniak - Dr Milica Vukasinovic-Vesic
More informationHair Testing to provide additional evidence in doping cases of potential (clenbuterol) contaminations
Hair Testing to provide additional evidence in doping cases of potential (clenbuterol) contaminations Detlef Thieme Institute of Doping Analysis, Dresden, Germany Beijing, Oct 2017 [M. Jahn (www.kreischa.net)]
More informationHigh speed UHPLC-MS/MS determination of multiple steroids in human serum using the Nexera MX system for multiplex analysis
PO-CON695E High speed UHPLC-MS/MS determination of multiple steroids in human serum using the Nexera MX system for multiplex analysis MSACL 6 EU Neil Loftus, Stephane Moreau, Mikaël Levi 3, Anja Grüning
More information26-G Keewaydin Drive, Salem, NH P: (800) F: (603)
Dihydrotestosterone ELISA For the quantitative determination of DHT in serum. For Research Use Only. Not for Use in Diagnostic Procedures. Catalog Number: 11-DHTHU-E01 Size: 96 wells Version: 4.1 August
More informationDECISION OF THE WORLD CURLING FEDERATION CASE PANEL. Dated 14 June, In respect of the following
DECISION OF THE WORLD CURLING FEDERATION CASE PANEL Dated 14 June, 2013 In respect of the following Adverse Analytical Finding: Case n. 2013/ 1301 Athlete /NF: Matt Dumontelle Member Association: Canadian
More informationSTD-3-V1M4_1.7.1_AND_ /15/2015 page 1 of 6. TNI Standard. EL-V1M4 Sections and September 2015
page 1 of 6 TNI Standard EL-V1M4 Sections 1.7.1 and 1.7.2 September 2015 Description This TNI Standard has been taken through all of the voting stages and has received consensus approval by the TNI membership.
More informationTrenbolone. Trenbolone Acetate Functions & Traits: Trenbolone Acetate
Trenbolone Trenbolone Acetate Trenbolone Acetate is an extremely powerful anabolic steroid and is considered the single greatest anabolic steroid by many performance enhancing athletes. This is one of
More informationEBVS are LESSONS FROM THE ASBP / 2. EBVs of Bulls Entered in the ASBP have provided a reliable Prediction of the Performance of their Progeny
EBVS are EBVs of Bulls Entered in the ASBP have provided a reliable Prediction of the Performance of their Progeny The Angus Sire Benchmarking Program (ASBP) has demonstrated that there is great potential
More informationDrugs and Ethics in Sport
Drugs and Ethics in Sport Ethics the practice of making principled choices between right and wrong explicitly reflecting on moral beliefs and practices Principles for an Ethical Framework Respect Basic
More informationDevelopment and validation of a simple, sensitive enzyme immunoassay for quantification of androstenedione in bull plasma
Mallick et al. Journal of Animal Science and Technology (2015) 57:13 DOI 10.1186/s40781-014-0035-z RESEARCH Open Access Development and validation of a simple, sensitive enzyme immunoassay for quantification
More informationAssessment of the Anabolic Effect of Androgens of the Edible Portion of the Shoot of Giginya Plant (Borassus aethiopum mart)
Publications Available Online J. Sci. Res. 2 (2), 362-368 (2010) JOURNAL OF SCIENTIFIC RESEARCH www.banglajol.info/index.php/jsr Assessment of the Anabolic Effect of Androgens of the Edible Portion of
More informationAnabolic Androgenic Steroids
Anabolic Androgenic Steroids 1 / 6 2 / 6 3 / 6 Anabolic Androgenic Steroids What are anabolic steroids? Anabolic steroids are synthetic, or human-made, variations of the male sex hormone testosterone.
More informationWADA Technical Document TD2018EAAS. Endogenous Anabolic Androgenic Steroids Measurement and Reporting
Endogenous Anabolic Androgenic Steroids Measurement and Reporting 1.0 Introduction The purpose of this Technical Document (TD) is to harmonize the approaches to the measurement and reporting of Endogenous
More informationIcd-10 low levels of testosterone
Icd-10 low levels of testosterone The Borg System is 100 % Icd-10 low levels of testosterone 1-10-2017 if your hormone levels are too high or too low, you may have a hormone disorder. Hormone diseases
More informationAntiduretic Hormone, Growth. Hormone & Anabolic Steroids
Goudarz Sadeghi, DVM, PhD, DSc Associate Professor of Pharmacology University of Tehran Faculty of Veterinary Medicine Veterinary Pharmacology Endocrine System Antiduretic Hormone, Growth Hormone & Anabolic
More informationab Androstenedione ELISA Kit
ab108672 Androstenedione ELISA Kit Instructions for Use A competitive immunoenzymatic assay for the quantitative measurement of Androstenedione in serum and plasma (citrate). This product is for research
More informationANNEX 3 TO THE DRAFT REPORT OF THE OECD VALIDATION OF THE RAT HERSHBERGER BIOASSAY: PHASE 2
ANNEX 3 TO THE DRAFT REPORT OF THE OECD VALIDATION OF THE RAT HERSHBERGER BIOASSAY: PHASE 2 Hershberger Assay Interlaboratory Study: Statistical analysis of phase 2 data from 16 laboratories in the multi-chemical
More informationMay 1, By John Heim,Doug Staples
1 of 7 7/11/2014 3:39 PM May 1, 2010 By John Heim,Doug Staples Anabolic steroid screening analysis in urine is complex and labor intensive requiring sensitive instrumentation and optimized chromatographic
More informationAustralian Supplement Survey Summary
Australian Supplement Survey Summary Introduction LGC, an established international life sciences measurement and testing company, has been analysing nutritional supplements for substances prohibited
More informationDonald W. Morrish, MD, PhD, FRCPC Presented at Mountain Man: Men's Health Conference, May Terry s Testosterone
Focus on CME at University of Alberta ADAM: Dealing with the Decline Donald W. Morrish, MD, PhD, FRCPC Presented at Mountain Man: Men's Health Conference, May 2005 We now know there is a decline in total
More informationPerformance Profiling: A Role for Sport Science in the Fight Against Doping?
TECHNICAL REPORT International Journal of Sports Physiology and Performance, 2009, 4, 129-133 2009 Human Kinetics, Inc. Performance Profiling: A Role for Sport Science in the Fight Against Doping? Yorck
More informationDrugs in Sport. Fifth Edition. Edited by David R. Mottram. Routledge. Taylor & Francis Group LONDON AND NEW YORK
Drugs in Sport Fifth Edition Edited by David R. Mottram Routledge Taylor & Francis Group LONDON AND NEW YORK Contents Contributors xv SECTION I The basis for and regulation of drug use in sport I 1 An
More informationGROWTH AND HORMONAL RESPONSE OF INTACT AND CASTRATE MALE CAlTLE TO TRENBOLONE ACETATE AND ESTRADIOL',*
GROWTH AND HORMONAL RESPONSE OF INTACT AND CASTRATE MALE CAlTLE TO TRENBOLONE ACETATE AND ESTRADIOL',* C. Y. Lee3, D. M. Henricks4, G. C. Skellefl and L. W. Grimes5 Clemson University, Clemson, SC 29634
More informationEXECUTIVE SUMMARY INTRODUCTION
EXECUTIVE SUMMARY INTRODUCTION This was a multi-institutional study on a topic yet to be addressed in South Africa. The first part of the study served as a pilot where the aim was to identify the presence/absence
More informationImport, seizure and analysis of prohibited doping products imported into Switzerland
Import, seizure and analysis of prohibited doping products imported into Switzerland Play the Game, 26-30 November, Eindhoven Christina Weber, PhD Introduction Since October 2012 new Sports Law in Switzerland
More informationCHEMILUMINESCENCE ENZYME IMMUNOASSAY (CLIA) TESTOSTERONE. Testosterone. Cat #
DIAGNOSTIC AUTOMATION, INC. 21250 Califa Street, Suite 102 and 116, Woodland Hills, CA 91367 Tel: (818) 591-3030 Fax: (818) 591-8383 onestep@rapidtest.com technicalsupport@rapidtest.com www.rapidtest.com
More informationBCAC ANTI DOPING POLICY
BCAC ANTI DOPING POLICY Version 1.5 May 14, 2014 ARTICLE 1 - DEFINITION OF DOPING For the purposes of this document, doping is defined as the occurrence of one (1) or more of the anti-doping policy contraventions
More informationMSD 96-Well MULTI-ARRAY CRP Assay
MSD 96-Well MULTI-ARRAY CRP Assay The following assay protocol has been optimized for analysis of C-reactive protein (CRP) in human serum and plasma samples. MSD Materials Storage Read Buffer T (4X), with
More informationA review of analytical strategies for the detection of endogenous steroid abuse in food production
Review Published online in Wiley Online Library (www.drugtestinganalysis.com) DOI 10.1002/dta.1354 A review of analytical strategies for the detection of endogenous steroid abuse in food production J.
More informationSection 3-5: Principle 3: Determine Critical Limits
Training Modules on General Food Safety Plans for the Food Industry Section 3-5: Principle 3: Determine Critical Limits Section Overview The third principle of HACCP is to determine the Critical Limits
More informationCatalogue no X. Cattle Statistics
Catalogue no. 23-012-X Cattle Statistics 2009 How to obtain more information For information about this product or the wide range of services and data available from Statistics Canada, visit our website
More informationMultiplex screening solutions for Growth Promoters and Synthetic Steroids
Multiplex screening solutions for Growth Promoters and Synthetic Steroids DISTRIBUIDOR / DISTRIBUTOR: ww Multiplex Drug Residue Screening Solution Randox present the worlds only multi-analyte semi-quantitative
More informationbiosensis Human Lipocalin-2/NGAL ELISA Kit Protocol
biosensis Human Lipocalin-2/NGAL ELISA Kit Protocol Catalog No: BEK-2141-2P For quantitative detection of human Lipocalin-2 in cell culture supernatants, serum, and heparin treated plasma, saliva, and
More informationDetectX TESTOSTERONE Enzyme Immunoassay Kit
DetectX TESTOSTERONE Enzyme Immunoassay Kit 1 Plate Kit Catalog Number K032-H1 5 Plate Kit Catalog Number K032-H5 Species Independent Sample Types Validated: Dried Fecal Extracts, Urine, Extracted Serum/Plasma,
More informationsignificant length of time without producing both anabolic
6 METABOLIC EFFECTS OF ANABOLIC STEROIDS* Professor V. WYNN Alexander Simpson Laboratory for Metabolic Research, St. Mary's Hospital Medical School, London, W.2. Everyone is familiar with the rapid weight
More informationFigure 2. RESULTS DATA ANALYSIS
ANDROGEN PARAMETERS IN HIRSUTE AND NORMAL FEMALE PATIENTS: IS THERE A ROLE FOR THE FREE ANDROGEN INDEX (FAI)? Castracane VD 1, Childress E 1, Tawwater B 1, Vankrieken L 2, El Shami AS 2 ( 1 Department
More information67th Annual Norfolk Beef Expo Open to the Entire State
67th Annual Norfolk Beef Expo Open to the Entire State Date: Sunday, September 11, 2016 Place: Northeast Community College Chuck M. Pohlman Ag Complex, located on East Benjamin Avenue Sponsor: Norfolk
More informationM. G. Keane, Teagasc, Grange Beef Research Centre, Dunsany, Co. Meath, Ireland.
European Association for Animal Production, Dublin 2007, Session 11, paper 7. Comparison of Friesian, Aberdeen Angus x Friesian and Belgian Blue x Friesian steers for beef production M. G. Keane, Teagasc,
More informationTestosterone ELISA Kit
Testosterone ELISA Kit Catalog Number KA3396 96 assays Version: 03 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General
More informationCREATININE AND CREATINE IN MUSCLE EXTRACTS.
CREATININE AND CREATINE IN MUSCLE EXTRACTS. II. THE INFLUENCE OF THE REACTION OF THE MEDIUM ON THE CREATININE-CREATINE BALANCE IN INCUBATED EXTRACTS OF MUSCLE TISSUE OF THE ALBINO RAT. BY FREDERICK S.
More informationbiosensis Rat Fibronectin ELISA Kit Protocol
biosensis Rat Fibronectin ELISA Kit Protocol Catalog No: BEK-2017-2P For quantitative detection of rat Fibronectin in cell culture supernatants, serum, and citrate, heparin, or EDTA plasma samples only
More informationKNG1 (Human) ELISA Kit
KNG1 (Human) ELISA Kit Catalog Number KA1040 96 assays Version: 07 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the Assay... 3 General
More informationF7 (Human) Chromogenic Activity Assay Kit
F7 (Human) Chromogenic Activity Assay Kit Catalog Number KA0971 96 assays Version: 02 Intended for research use only www.abnova.com Table of Contents Introduction... 3 Background... 3 Principle of the
More informationSteroids and the Internet: Do You Know What You re Up Against?
Steroids and the Internet: Do You Know What You re Up Against? Don Melrose PhD CSCS*D Associate Professor of Kinesiology TAMUCC NSCA Texas State Director 1 Forward This is NOT a how to guide to obtaining
More informationTrace-Level Analysis of Metanephrines in Plasma by HILIC LC-MS/MS
Abstract Highly sensitive analysis of metanephrines in plasma is critical in the diagnosis and treatment of pheochromocytoma and paraganglioma. Here, a HILIC LC-MS/MS method was developed using a Raptor
More informationTESTOFEN HUMAN CLINICAL TRIAL GENCOR PACIFIC, INC. Copyright 2006 by Gencor Pacific, Inc.
GENCOR PACIFIC, INC. 920 E. Orangethorpe Avenue, Suite B, Anaheim, CA 92801 Ph: 714.870.8723 714.870.8724 efax: 732.875.0306 drjit@gencorpacific.com gita@gencorpacific.com www.gencorpacific.com TESTOFEN
More informationHair analyses. Hair analysis beyond workplace testing. Ia: Fatal steroid abuse. Identification of stanozolol in hair
Hair analyses Hair analysis beyond workplace testing Detlef Thieme IDAS Kreischa IRM + FTC Munich. anabolic (steroids, clenbuterol). kinetic (segmentation and profiling) 3. genetic (amitriptyline vs.nortriptyline
More informationPeninsula Laboratories International, Inc. 305 Old County Rd., San Carlos, CA 94070, USA Tel: (650) Fax: (650)
EIA Protocol Peninsula Laboratories International, Inc. 305 Old County Rd., San Carlos, CA 94070, USA Tel: (650) 801-6090 Fax: (650) 595-4071 Peptide Enzyme Immunoassay (EIA) Protocols EIAH - high sensitivity
More informationRayBio Human vwf ELISA Kit
RayBio Human vwf ELISA Kit Catalog #: ELH-vWF User Manual Last revised April 15, 2016 Caution: Extraordinarily useful information enclosed ISO 13485 Certified 3607 Parkway Lane, Suite 100 Norcross, GA
More informationWe Are For Clean Basketball
We Are For Clean Basketball What is doping? DOPING is the use of substances and methods intended to improve the performance of a player. It poses a risk to health. It is contrary to sporting ethics. Doping
More informationWe Are For Clean Basketball
We Are For Clean Basketball What is doping? DOPING is the use of substances and methods intended to improve the performance of a player. It poses a risk to health. It is contrary to sporting ethics. Doping
More informationSex Steroids in Scleractinian Coral, Euphyllia ancora: Implication in Mass Spawning 1
BIOLOGY OF REPRODUCTION 68, 2255 2260 (2003) Published online before print 22 January 2003. DOI 10.1095/biolreprod.102.012450 Sex Steroids in Scleractinian Coral, Euphyllia ancora: Implication in Mass
More informationWADA Health, Medical & Research Committee Meeting May 2001 Stockholm SWEDEN. Minutes
WADA Health, Medical & Research Committee Meeting 27-28 May 2001 Stockholm SWEDEN Minutes 1. Meeting Commencement, Review of Agenda Members present: Arne Ljungqvist Chair Barbara Drinkwater Bengt Saltin
More information