Physician Copy. Patient: SAMPLE PATIENT DOB: Sex: MRN: ACH24.1
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1 Physician Copy Patient: SAMPLE PATIENT DOB: Sex: MRN: ACH24.1
2 ID: Steroidogenic Pathway At-A-Glance RR : RR : RR : RR : RR : RR : RR : RR : RR : <= RR : RR : RR : RR : RR : RR : RR : RR : <=5.9 RR : <=1.0
3 ID: Interpretation At-A-Glance Anabolic/Catabolic Balance 17-Ketosteroids/17-Hydroxysteroids Ratio Anabolic/Catabolic Balance (24hr urine) 0.3 Catabolic (Wear & Tear) Anabolic (Growth & Healing) Catabolic 17 - Hydroxysteroids 26.7 Total* * Total values equal the sum of all measurable parts micromol/24 hr Anabolic 17 - Ketosteroids Total* micromol/24 hr Enzymatic Activity Estrogen Metabolism Phase 1 Phase 2 Lower 2/16 Ratio More Methylation Estrogen Metabolism 2-OH (E1+E2) / 16α-OHE1 RR : Methylation Activity 2-OH (E1+E2) / 2-MeO (E1+E2) 0.8 RR : Higher 2/16 Ratio Less Methylation 2-OH(E1+E2) 4-OH(E1+E2) 2-MeO(E1+E2) 4-MeO(E1+E2) 16α-OHE1 5α-Reductase Activity Etiocholanolone/Androsterone (E/A) Ratio More 5α-Reductase 0.4 Less 5α-Reductase RR : This sample pie-chart reflects current scientific understanding of the association of specific estrogen metabolites with disease risk for hormone related cancers. Metabolites in green have been associated in the literature with decreased risk; those in red, with increased risk. 16-OHE1 (in yellow) has mixed findings, some studies showing an association and many finding no association. The dark line separates Phase 1 and Phase 2 detoxification pathways. Key
4 Complete Hormones (24hr) Patient: SAMPLE PATIENT DOB: Sex: MRN: Methodology: GC-MS and LC-MS/MS; Specimen: 24 hour urine; Results normalized to volume Progesterone Pregnanediol (24hr urine) micromol/24 hr Androgens 17-Ketosteroids DHEA (24hr urine) 0.10 Androsterone (24hr urine) 3.69 Etiocholanolone (24hr urine) Keto-androsterone (24hr urine) 0 11-Keto-etiocholanolone (24hr urine) Hydroxy-androsterone (24hr urine) Hydroxy-etiocholanolone (24hr urine) Ketosteroids, Total* (24hr urine) 9.3 * Total values equal the sum of all measurable parts Testosterone (24hr urine) 0.10 Androstanediol (24hr urine) micromol/24 hr micromol/24 hr micromol/24 hr micromol/24 hr micromol/24 hr micromol/24 hr micromol/24 hr micromol/24 hr micromol/24 hr <= 5 micromol/24 hr Glucocorticoids 17-Hydroxysteroids Pregnanetriol (24hr urine) 1 allo-tetrahydrocortisol, a-thf (24hr urine) 2.47 Tetrahydrodeoxycortisol, THS (24hr urine) 0.17 Tetrahydrocortisone, THE (24hr urine) Tetrahydrocortisol, THF (24hr urine) Hydroxysteroids, Total* (24hr urine) 26.7 * Total values equal the sum of all measurable parts micromol/24 hr micromol/24 hr <= 1.00 micromol/24 hr micromol/24 hr micromol/24 hr micromol/24 hr
5 Methodology: LC-MS/MS; Specimen: 24 hour urine; Results normalized to creatinine Estrogens ID: Page 2 Estrogens Estrone (E1)* mcg/g Creat. * (luteal) reference range shown mcg/g Creat mcg/g Creat mcg/g Creat. Estradiol (E2) * 2.4 * (luteal) reference range shown mcg/g Creat mcg/g Creat mcg/g Creat mcg/g Creat. Estriol (E3) * * (luteal) reference range shown Estrogen Metabolites 2-Hydroxyestrone + 2-Hydroxyestradiol [2-OH(E1+E2)] * 0.4 * (luteal) reference range shown mcg/g Creat mcg/g Creat mcg/g Creat mcg/g Creat mcg/g Creat mcg/g Creat mcg/g Creat mcg/g Creat. 16α-Hydroxyestrone (16α-OH E1) * 2.2 * (luteal) reference range shown mcg/g Creat mcg/g Creat mcg/g Creat. <=2.0 mcg/g Creat. 4-Hydroxyestrone+4-Hydroxyestradiol [4-OH(E1+E2)] * 8.3 <= 5.9 mcg/g Creat. * (luteal) reference range shown <=5.9 mcg/g Creat. <=8.8 mcg/g Creat. <=1.6 mcg/g Creat. 2-Methoxyestrone+2-Methoxyestradiol [2MeO(E1+E2)]* 0.5 * (luteal) reference range shown -8.6 mcg/g Creat mcg/g Creat mcg/g Creat mcg/g Creat. 4-Methoxyestrone+4-Methoxyestradiol [4MeO(E1+E2)]* 0.3 * (luteal) reference range shown <= 1.0 mcg/g Creat. <=1.0 mcg/g Creat. <=1.0 mcg/g Creat. <=1.0 mcg/g Creat.
6 Methodology: LC-MS/MS; Specimen: 24 hour urine Ratios ID: Page 3 Estrogens Anabolic/Catabolic Balance (24hr urine) E/A: 5β/5α Ratio (24hr urine) OH(E1+E2) / 16α-OHE1* * (luteal) reference range shown OH(E1+E2) / 2-MeO(E1+E2) * 0.8 * (luteal) reference range shown Lab Comments The performance characteristics of all assays have been verified by Genova Diagnostics, Inc. Unless otherwise noted with, the assay has not been cleared by the U.S. Food and Drug Administration. Please note the reference range for Tetrahydrocortisone (THE), Total 17-OH Corticosteroids, and the Anabolic/Catabolic Balance have been updated. Please note analysis of estrogens and estrogen metabolites is now performed using LC/MS/MS. The reference ranges for these biomarkers have been updated.
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