iba World of IBA Oligos HIGH QUALITY DNA AND RNA CUSTOM SERVICES FLUORESCENT LABELS Solutions For Life Sciences
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1 iba Solutions For Life Sciences World of IBA Oligos HIGH QUALITY DNA AND RNA CUSTOM SERVICES quality, expertise and innovation FLUORESCENT LABELS NON-FLUORESCENT MODIFICATIONS BACKBONE MODIFICATIONS
2 A GERMAN LEADER IN THE SYNTHESIS OF SPECIAL NUCLEIC ACIDS SINCE 1991 IBA produces specialized, modified and labor-intensive nucleic acid products. Our highly motivated and qualified team not only has special expertise in DNA and RNA modification and labeling, but our in-house nucleoside and nucleotide chemistry laboratory continuously develops new modifications, not available elsewhere. 2 You ask for it we synthesize it! Just tell us what you need.
3 Chimeric oligos all combinations of: DNA RNA 2 -O-Methyl-RNA 2 -Fluoro-RNA 2 -Amino-RNA WE PROVIDE DNA Standard DNA oligonucleotides Long range up to 200 bases* Large scale oligonucleotides 3 -, 5 - or internally modified RNA dsrna, sirna, mirna, random RNA Long range up to 100 bases* 3 -, 5 - or internally modified 2 -modified: -O-Me, -F, -NH 2 Nucleoside Triphosphates RNA and RNA/DNA hybrid dinucleotides *depending on sequence and scale We were always fully satisfied with the exceptional quality, purity and reliability of the amount of unlabeled and labeled oligonucleotides, as well as with the customer support provided by IBA. Prof. Dr. Markus Sauer, Biotechnology & Biophysics, University of Wuerzburg, Germany. 3
4 FLUORESCENT LABELS For dual and multiply labeled DNA and RNA More than 200 labels to choose from Covering the entire spectrum from ultraviolet to infrared Stokes shifts up to 180 nm Licensed supplier of oligos labeled with Alexa Fluor (Molecular Probes, owned by life technologies) Alternative dyes from Atto-Tec, Luminartis, Abberior, Dyomics and BaseClick Quenchers such as BBQ and BHQ 4 Fluorescent labels Atto Alexa Fluor Cyanine Dy FAM TET JOE Eterneon Abberior Chromeo BHQ /BBQ quenchers and many more! CLICK N PLAY Request a poster with our available fluorescent labels at oligobrochure@ iba-lifesciences.com Excellent high quality and competitive prices e.g. for FRET, qpcr, FCS/FCCS, confocal and STED microscopy, single-molecule imaging, multi-color DNA and RNA FISH, DNA Origami NEW FOR MULTI-COLOR APPLICATIONS! We synthesize oligos using the Click-Chemistry, allowing completely new dye combinations not previously available and at so far unreachable labeling densities.
5 NON-FLUORESCENT MODIFICATIONS Phosphorothioate Bond Base More than 80 internal, 5 - and 3 - DNA and RNA modifications to choose from O=P S Including Biotin, Iminobiotin, Desthiobiotin and Digoxigenin For DNA microarrays Surface binding and hybridization experiments Cross-linking and crystallography studies And more! Base Request a brochure with our available nonfluorescent modifications at oligobrochure@ iba-lifesciences.com BACKBONE MODIFICATIONS Non-fluorescent modifications PHOSPHOROTHIOATES For antisense applications, we offer the nuclease-resistant Phosphorothioates (PTOs) for DNA and RNA. Introduced at either the 5 - or 3 -end of an oligo, Phosphorothioates can inhibit exonuclease degradation. If they are introduced internally, they can limit attacks by endonucleases. Bromo Amino Deaza Carboxyl Phosphate Psoralen Biotin Desthiobiotin Digoxigenin Methyl Glycol HEGL Thiol DBCO Spacers Iminobiotin and many more! 5
6 APTAMERS AN ALTERNATIVE TO ANTIBODIES With our expertise in high quality oligonucleotide synthesis we offer custom synthesis of DNA as well as RNA aptamers, depending on your needs. Due to their specificity, high affinity, low immunogenicity and ease of use, aptamers have been widely used in the medical and industrial fields of disease therapy, cell therapy, drug delivery, drug discovery, diagnostics, biosensors and bioimaging. When we started to work with aptamers, a collaborator recommended IBA for RNA synthesis and modification. Ever since we have been completely satisfied with IBA s RNA and aptamer quality and appreciate the company s excellent customer service. 6 C.F.M. Sier, Oncologic Surgery, Leiden University Medical Center, The Netherlands Hit your target with DNA or RNA aptamers!
7 Price assembly 4 STEPS TO ORDER YOUR OLIGO Number of bases Purification* Label/Modification *HPLC is included for modified/ labeled DNA u Choose your scale v List sequence 5 to 3 w Indicate modification type and position x Select purification method Ready to go! Scales available and typical yields Typical yields of an unmodified 20mer* (DNA and RNA) Synthesis scale Molecular Biology Grade After HPLC 0.01 μmol 2 3 nmol 1 2 nmol 0.05 μmol nmol 5 8 nmol 0.20 μmol nmol nmol 1.00 μmol nmol nmol μmol 1,000 1,500 nmol 600 1,000 nmol μmol 2,000 3,500 nmol 1,500 3,000 nmol *with equal base composition Oligo-Shop Orders oligoorder@iba-lifesciences.com 7
8 For Life Sciences Product Abs em e Alternative for [cm -1 M -1 ] Azide for Labeling via Click- Chemistry* Pyrene ) Dansyl-X Alexa Fluor AMCA -X Alexa Fluor 350 1) Eterneon 350/ ) Eterneon 350/ Dy 350 NEW Alexa Fluor 350 1) Eterneon 384/ ATTO ) Eterneon 393/ ) Eterneon 394/ Dy 405 NEW Alexa Fluor 405 Alexa Fluor Oyster 405 NEW Alexa Fluor 405 Hydroxy coumarin (HYCO) Dy Alexa Fluor 430 DyQ 425 NEW 425 none D-AMCA Alexa Fluor 430 1) ATTO Abberior STAR 440SX 1) NEW BD Horizon V500 ATTO LC 480 Cyan 500 1) Abberior STAR 470SX NEW Dabcyl 478 none ) Eterneon 480/ ) Dy 485 XL Chromeo 488 NEW Alexa Fluor 488 Oyster Cy2 Alexa Fluor 488 Dy 490 NEW Fluorescein-5-EX BHQ none FAM, 5-isomer Dy Chromeo 494 NEW ) Alexa Fluor ATTO Alexa Fluor 488 FAM, 6-isomer IBApy 493/503 1) ATTO Cy2 Alexa Fluor 488 Abberior STAR 488 NEW IBApy FL 1) Alexa Fluor 500 NEW DyQ 505 NEW 504 none ) Dy 480 XL Rhodamine 110X Dy RhodamineGreen TM 1) Dy 510 XL ATTO 514 NEW Abberior STAR 512 NEW ATTO JOE TM, TET TM Alexa Fluor 514 NEW ) Dy 481 XL JOE, 6-isomer TET ) Dy 520 XL Carboxy-rhodamine 6G, isomer Carboxy-rhodamine 6G, isomer 1) Dy 521 XL ) IBApy R6G Alexa Fluor ATTO Alexa Fluor 532 HEX Dy 530 NEW Alexa Fluor 532 1) IBApy 530/ BHQ none ATTO Rho6G NEW VIC HEX ATTO 540Q 542 none DyQ 1 NEW 543 none ) IBApy TMR-X Dy Alexa Fluor 546 TAMRA, 5-isomer Dy Quasar Cy3, Bodipy TMR-X Custom oligonucleotides labeled via Click Chemistry Product Abs em e Alternative for [cm -1 M -1 ] Azide for Labeling via Click- Chemistry* Dy Chromeo 546 NEW Cy3 Oyster Cy3, Alexa Fluor 555 Oyster Cy3, Alexa Fluor 555 ATTO Cy3, Alexa Fluor 555, NED Alexa Fluor 3) Cy3 Cy3 NHS Cy TAMRA, 6-isomer Alexa Fluor Cy3 Dy Dy Cy3 Dy Dy 550 NEW Dy 549 NEW Dy 549P1 NEW Alexa Fluor 555 ATTO Cy3.5, ROX TM, Product Abs em e Alternative for [cm -1 M -1 ] Azide for Labeling via Click- Chemistry* Cy ChromeoTM 642 NEW Dy Oyster Cy5, Dy Oyster Cy5, Dy Cy5 Dy Dy 654 NEW Dy 649P1 NEW Dy Dy Dy Dy 649 NEW ATTO MB2 NEW 658 none DyQ none DyQ none Bodipy 568 ATTO 655 (hydrophil) Cy5, ATTO Rho3B NEW Rhodamine Red X, Oyster Alexa Fluor 568 ATTO Oxa12 (lipophil) NEW Cy5, ATTO Rho11 NEW ATTO 665 NEW Carboxy-X-rhodamine, Alexa Fluor Alexa Fluor 6-isomer 568 Methylene Blue (on request) ATTO Rho12 NEW BHQ none Carboxy-X-rhodamine, Cy5.5 NHS Alexa Fluor 5-isomer 568 Cy Alexa Fluor ROX TM Dy BHQ none Dy 678 NEW ATTO Thio12 NEW Dy 675 NEW Dy 591 NEW Dy Dy Oyster Cy5.5, Abberior STAR 580 NEW Alexa Fluor 680 Sulforhodamine Dy 679P1 NEW ATTO 580Q 586 none Dy 679 NEW ATTO Rho101 NEW ROX TM Alexa Fluor Cy5.5 Cy3.5 Alexa Fluor ,, ATTO Alexa Fluor LightCycler , Alexa Fluor 700 Cy3.5 NHS DyQ 3 NEW 683 none Cy Alexa Fluor 594 Dy Cy5.5 Dy 594 NEW Alexa Fluor 594 Dy Alexa Fluor 680 Oyster 594 NEW Alexa Fluor 594 Dy ATTO Alexa Fluor 594 DyQ 700 NEW 696 none BBQ none ATTO Cy5.5, Dy 605 NEW Alexa Fluor 610 LightCycler Red 705 ATTO Rho13 NEW Alexa Fluor LightCycler Red 705 ATTO Alexa Fluor 594 Dy 703 NEW Alexa Fluor 610-X NEW Dy 704 NEW Dy Alexa Fluor 610 Dy Alexa Fluor Dy Alexa Fluor 700 ATTO Alexa Fluor 610, ATTO LightCycler Red 610 Dy ATTO 612Q 615 none Dy ATTO Dy Dy Dy ATTO Rho14 NEW ATTO Cy7, ATTO Alexa Fluor 633 Alexa Fluor 740 Alexa Fluor Oyster 750 NEW Cy7, Alexa Fluor 635 NEW Alexa Fluor 750 Abberior STAR 635 NEW Dy 754 NEW Alexa Fluor 750 Abberior STAR 635P NEW Alexa Fluor EVOblueTM Dy Dy Alexa Fluor 633 Cy7 NHS Dy Dy Cy7 Dy Dy Cy7 Dy Dy 749P Dy DyQ 4 NEW 766 none DyQ 2 NEW 641 none Dy 778 NEW ATTO 647-N Cy5, Dy 777 NEW Dy Quasar Cy5, Alexa Fluor Dy , Bodipy 650/665 Dy ATTO 647 NEW Cy5, Dy Alexa Fluor 790 NEW Cy5 NHS Dy ) dyes with large Stokes shift, 2) only available with an additional Reverse Phase HPLC, 3) only available in combination with PAGE purification A new oligonucleotide labeling philosophy allows completely new dye combinations not available before so far unreachable labeling densities very reliable labeling high yield of completely labeled product combination with existing labeling techniques for greater diversity oligo@iba-lifesciences.com IBA GmbH Rudolf-Wissell-Str Goettingen Germany Phone Fax info@iba-lifesciences.com IBA US Distribution Center 1328 Ashby Road Olivette MO USA Phone IBA-GmbH ( ) Fax info@iba-lifesciences.com We were always fully satisfied with the exceptional quality, purity and reliability of the amount of unlabeled and labeled oligonucleotides as well as with the customer support provided by IBA. Prof. Dr. Markus Sauer, Biotechnology & Biophysics, University Würzburg, Germany Non-fluorescent labels Oligo Shop Orders oligoorder@iba-lifesciences.com Requests oligoexpert@iba-lifesciences.com Oligo Support Tel. +49 (0) For licensing and trademark information please refer to Photography: Eva-Maria Twehues iba Solutions Fluorescent labels For Dna and rna iba Solutions For Life Sciences Product information NON-FLUORESCENT LABELS FOR OLIGONUCLEOTIDES Modifications for DNA and RNA For licensing and trademark information please refer to * NEW! Click and Play. Grade up your Oligo! Poster with fluorescent labels Brochure with nonfluorescent modifications IBA GmbH Rudolf-Wissell-Str Goettingen Germany Tel Fax
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