Hatchery and Supplementation Program DRAFT 2014 Report. Lewis River Hydroelectric Projects FERC Project Nos. 935, 2071, 2111, 2213

Transcription

1 Hatchery and Supplementation Program DRAFT 2014 Report Lewis River Hydroelectric Projects FERC Project Nos. 935, 2071, 2111, 2213 Erik Lesko April 2015

2 TABLE OF CONTENTS 1.0 INTRODUCTION WINTER STEELHEAD Broodstock Collection Merwin Trap Tangle Netting Cedar Creek Winter Steelhead Broodstock Collection Timing Genetic Analysis of Potential Broodstock Genetic Analysis of BWT Steelhead Returns to the Merwin Trap Spawning and Egg Take Rearing, Tagging and Release Rearing Tagging Release MONITORING AND EVALUATION Winter Steelhead Redd Surveys (Lower River) Spawning Abundance Distribution Double Floy tagged steelhead returns to the Merwin Trap Ratio of NOR to HOR steelhead in the traps and tangle netting Upstream Transport of Winter Steelhead Coho and Spring Chinook Salmon Redd Surveys Coho and Spring Chinook Salmon 2013: Coho and Spring Chinook Salmon 2014: Screw trap Operations Mainstem Carcass Surveys Coho and Spring Chinook Salmon Coho Salmon Tributary Surveys 2012: Coho Salmon Surveys 2013: Spring Chinook Surveys Coho Salmon Surveys Spring Chinook Surveys 2014: i

3 4.0 RECOMMENDATIONS FOR ONGOING MANAGEMENT REFERENCES APPENDICES Appendix A Wild Winter Steelhead Collection Log 2014 Appendix B Genetic Assignment Results from Lewis River Captures at Merwin Trap (MT), Cedar Creek (CC) and Tangle Netting (TN) 2014 Appendix C Wild Winter Steelhead Spawning Log 2014 Appendix D Wild Winter Steelhead Spawning Crosses 2014 Appendix E Steelhead Redd locations, Lewis River, WA 2014 TABLES TABLE 1. COLLECTION SUMMARY OF WINTER STEELHEAD CAPTURES BY LOCATION BETWEEN 2009 AND TABLE 2. DISPOSITION OF WINTER STEELHEAD CAPTURED AT THE MERWIN TRAP (AND LEWIS LADDER) BETWEEN JANUARY 13 AND JUNE 13, TABLE 3. DISPOSITION OF STEELHEAD CAPTURED THROUGH TANGLE NETTING IN TABLE 4. NUMBER OF SPAWNING CROSSES AND PARENTS INCLUDING THE DURATION OF EACH SPAWNING PERIODS FOR BROOD YEARS BETWEEN 2009 AND TABLE 5. SUMMARY OF REARING STATISTICS FOR THE 2014 BROOD YEAR TABLE 6. WILD WINTER STEELHEAD ABUNDANCE DOWNSTREAM OF MERWIN DAM 2008 THROUGH 2014 BASED ON REDD COUNTS TABLE 7. REDD DISTRIBUTION SUMMARY FOR REACHES 1 THROUGH 5 INCLUDING REDDS PER MILE TABLE 8. RATIO OF HOR AND NOR STEELHEAD CAPTURED IN THE TANGLE NET FROM MARCH 1 TO MAY 12, 2014 (N=148) TABLE 9. SUMMARY OF WINTER STEELHEAD TRANSPORTED AND RELEASED UPSTREAM OF SWIFT DAM TABLE 10. COLLECTION SUMMARY FOR THE ROTARY SCREW TRAP DEPLOYED DOWNSTREAM OF MERWIN DAM APRIL 1 JUNE 11, TABLE 11. DISPOSITION OF CAPTURED FISH (EXCLUDING FRY) INCLUDING CATCH RATES OF BWT AND NON BWT RAINBOW FORM APRIL 1 JUNE 11, ii

4 FIGURES Figure 1. Frequency of winter steelhead captured at the Merwin fish trap between January 1, 2014 and June 13, Figure 2. Composition of winter steelhead captured through tangle netting between February 14, 2014 and May 12, 2014 (n= 162) Figure 3. Actual collection timing of steelhead used as broodstock compared to predetermined collection curve during the 2014 season Figure 4. Proportion of primary, secondary and tertiary genetic assignment of potential steelhead broodstock sampled in 2014 at the Merwin Trap, tangle netting and Cedar Creek Weir (n=163) Figure 5. Proportion of primary, secondary and tertiary genetic assignment of BWT steelhead transported upstream of Swift Dam from the Merwin Trap in 2014 (n=35)... 9 Figure 6. Number and timing of winter steelhead (males and females) spawned at Merwin hatchery (n=51) in Figure 7. Observed mortality rate of fish ponded for brood years 2009 through Figure 8. Wild winter steelhead spawner abundance downstream of Merwin Dam 2008 through 2014 based on redd counts iii

5 1.0 INTRODUCTION The purpose of this report is to document results from field studies associated with implementation of the Hatchery and Supplementation (H&S) Program during Monitoring and evaluation (M&E) activities of the H&S program are planned in consultation with the H&S subgroup and incorporated into the Annual Operating Plan (AOP) each year. In 2014, the following key activities were completed as part of the 2014 AOP: Transport of winter steelhead and early coho salmon upstream of Swift Dam Screw trapping of emigrating juveniles downstream of Merwin Dam Carcass and redd surveys downstream of Merwin Dam Broodstock collection and production for wild winter steelhead supplementation Hatchery production of trout and salmon as stipulated in Section 8 of the Lewis River Settlement Agreement. This report is required by Section of the Lewis River Settlement Agreement that states: On an annual basis, the Licensees shall provide to the ACC for review and comment a report compiling all information gathered pursuant to implementation of the Hatchery and Supplementation Plan. The report also will include recommendations for ongoing management of the Hatchery and Supplementation Program. The ACC shall have 60 days to comment on the annual report. Within 60 days of the close of the comment period, the Licensees shall finalize the report after consideration of all comments. The Licensees shall also provide the comprehensive periodic review undertaken pursuant to Section below to the ACC. The Licensees shall provide final annual reports and the comprehensive periodic review to the Services during the development of any required ESA permit or authorization for hatchery operations, including NOAA Fisheries HGMP process. The report may be included as part of the detailed annual reports of the ACC activities required by Section

6 2.0 WINTER STEELHEAD The winter steelhead program is composed of three elements: (1) a broodstock capture program with a goal to collect up to 50 natural origin broodstock; (2) a spawning and rearing program that targets a release of 50,000 smolts each year; and finally (3) a transport program that moves all program steelhead returns upstream of Swift Dam. The primary goal of this program is to support a self sustaining population upstream of Swift Dam that in time requires no hatchery support. A summary of steelhead collected for years 2010 through 2014 is provided in Table 1. Table 1. Collection summary of winter steelhead captures by location between 2009 and YEAR Merwin Trap ,075 Lewis River Trap Cedar Creek Weir Tangle Netting Angling 8 2 Discontinued in 2011 TOTAL , Broodstock Collection Broodstock collection relied principally on two methods: (1) trapping at Merwin Dam and (2) tangle netting. Cedar Creek weir contributed one broodstock and Lewis Ladder contributed one NOR that was later released. All captured NOR winter steelhead were transported to Merwin Hatchery for genetic assignment analysis. After results were known, these fish were either held for broodstock or released back to river depending on predetermined collection curves. All program fish (BWT) capured in the Merwin Trap were immediately transported upstream of Swift Dam as part of the supplementation program. Data for all steelhead transported to the Merwin hatchery is provided in Appendix A Merwin Trap The first unclipped steelhead was collected at the Merwin trap on March 23, 2014; the first BWT steelhead was captured on January 13, During the period January 13 June 13, twenty nine (29) unclipped NOR steelhead and 1,059 BWT winter steelhead were captured at the trap. Of all BWT steelhead captured, 1033 were transported upstream of which 82 BWT steelhead received a Lotek radio transmitter prior to release upstream. Of the 29 NOR s captured, 18 were spawned and 11 were released back to river. 2

7 Table 2. Disposition of Winter Steelhead Captured at the Merwin Trap (and Lewis Ladder) between January 13 and June 13, RETAINED Males Females Spawned 8 10 Released* 9 2 RELEASED Upriver Transport (BWT) Upriver Transport (Radio Tagged) NOR Kelts (to river) 4 1 Mortalities 4 4 Lewis River Ladder 1 0 TOTAL * Released from Merwin Hatchery after genetic assignment results were known 350 Number of Steelhead BWT Males BWT Females NOR's 50 0 Jan Feb Mar Apr May Jun Month NOTE: All BWT steelhead are considered HOR Figure 1. Frequency of winter steelhead captured at the Merwin fish trap between January 1, 2014 and June 13,

8 2.1.2 Tangle Netting Tangle netting efforts began on February 14, 2014 and continued through May 12, A PacifiCorp crew of two biologists went out once per week depending on river conditions. Six to eight pound test monofilament, 4 inch (stretch) mesh tangle nets were drifted in known and established steelhead holding areas. Once a steelhead became entangled in the drifting net it was pulled into the boat, freed and then placed in an insulated cooler with fresh river water. All steelhead were processed on the boat and either released or transported to an oxygenated holding tank at the Lewis River hatchery access area. Table 3 summarizes the disposition of steelhead captured during tangle netting efforts. Table 3. Disposition of steelhead captured through tangle netting in Males Females RETAINED NOR Spawned *NOR Released (from Merwin) 15 3 HOR (Euthanized) 1 0 RELEASED (On Site) NOR kelts HOR Kelts 0 3 BWT (double floy) 13 1 Not needed 17 0 Residuals 20 0 Recaptures 13 1 Other 4 2 Mortalities 0 0 TOTAL * Released from Merwin Hatchery after genetic assignment results were known or received as kelts In total, 162 steelhead were captured through the tangle netting program. Of these, 112 (69%) were of natural origin (Figure 2). Remaining fish were either program returns (BWT) or from traditional hatchery programs. 4

9 40 HOR NOR 30 Number of Steelhead Feb Mar 1-15 Mar Apr 1-15 Apr May 1-12 Period Figure 2. Composition of winter steelhead captured through tangle netting between February 14, 2014 and May 12, 2014 (n= 162) Cedar Creek Only three steelhead were transported from the Cedar Creek Weir to Merwin Hatchery. One fish was spawned, one fish was released back to river and the last steelhead was a mortality at the hatchery after being held for 24 days. 2.2 Winter Steelhead Broodstock Collection Timing The ability to conform to predetermined collection curves presents several difficulties in the field. Specific issues continue to make broodstock collection problematic. These specific issues include the following: More fish (than stipulated in the collection curve) need to be captured each period to ensure assignment analysis will not reduce available broodstock below target levels 5

10 Spawning maturity is a significant variable between males and females during spawning and adds uncertainty when deciding to retain or release broodstock. This is most notable in females rather than males. Capture efficiency is affected by river conditions which can change weekly if not daily Gender ratios need to be managed and maintained to ensure adequate number of crosses and limited multiple use of broodstock males Steelhead condition varies throughout the collection period with a larger percentage of fish being returned to river later in the collection window due to ripeness, and, Fecundity varies substantially from fish to fish and from year to year. Because of the many unknowns with collecting live fish in their natural environment, the collection curve is intended to be a guide for collection crews to help plan fish collection activities on a weekly basis. This planning helps to ensure that fish are collected across their spawning period and helps ensure genetic diversity among the available broodstock. The first steelhead retained for broodstock was on February 25, 2014 from tangle netting efforts. This fish was spawned during the first spawning event on April 7. Tangle netting efforts ended after May 12 because the broodstock collection goal had been achieved. According to the collection curve proposed in the annual plan for 2014, 50 steelhead are to be held for broodstock and spawned over the course of the run. In 2014, 51 steelhead (26 females and 25 males) were spawned. Capture timing closely matched the curve as provided in the annual plan (Figure 3) despite the challenges mentioned above Collection Curve Actual Broodstock Captures Number of Steelhead Jan - Feb Mar 1-15 Mar Apr 1-15 Apr May 1-15 Collection Period 6

11 Figure 3. Actual collection timing of steelhead used as broodstock compared to predetermined collection curve during the 2014 season. 2.3 Genetic Analysis of Potential Broodstock The H&S Subgroup agreed to use a genetic assignment target level of 50 percent or greater to the NF Lewis River or Cedar Creek stock(s) to be considered acceptable broodstock. Additionally, steelhead captured after April 1 with 50 percent or greater assignment to the Cascade Strata are also considered acceptable broodstock. The only exception to this rule is for fish showing hatchery assignment at levels greater than 5 percent. These fish would not be incorporated into the broodstock despite any assignment of 50 percent or greater to the NF Lewis River wild winter steelhead stock or Cascade Strata. A total of 163 samples were taken from steelhead captured in the Merwin Trap, through tangle netting and the Cedar Creek Weir. All sampled steelhead were assigned a probability percentage as to likelihood of assignment to known baselines established for lower Columbia River tributaries. Probabilities are classified as primary, secondary and tertiary to account for introgression from other basins and provide a more complete picture of diversity present within the samples. Figure 4 provides an illustration of results of sampled (n=163) non BWT steelhead. Appendix B provides the tabular results for each individual unclipped steelhead captured at the Merwin trap, tangle netting and at the Cedar Creek weir. 7

12 0.60 NF Lewis Cedar Germany Coweeman Grays EF Lewis Mill Kalama Winter Sandy Kalama Summer SF Toutle Skamania Hatchery Green Lewis Hatchery Klickitat Summer Big Cr. Elochoman Hatchery Elochoman Kalama Hatchery Hood R. Clackamas Proportion of Samples Primary Assignment Secondary Assignment Tertiary Assignment Baseline Figure 4. Proportion of primary, secondary and tertiary genetic assignment of potential steelhead broodstock sampled in 2014 at the Merwin Trap, tangle netting and Cedar Creek Weir (n=163). 2.4 Genetic Analysis of BWT Steelhead Returns to the Merwin Trap Thirty five samples were obtained from BWT steelhead captured in the Merwin Trap in Figure 5 presents the results from a subsample (n=35) of steelhead captured in the Merwin Trap and transported upstream of Swift Dam to spawn naturally using primary, secondary and tertiary assignment probabilities. 1 A larger sample (n > 700) of BWT steelhead captured in the Merwin Trap over multiple years is currently being analyzed to quantify family representation of BWT steelhead returns. This analysis will be presented to the ACC once finalized. 8

13 0.60 Merwin Cedar Mill Clackamas Germany Cr Coweeman Green R Grays EF Lewis LR Hatchery Elochoman R Elochoman H Kalama Winter SF Toutle Proportion of Samples Primary Secondary Tertiary Baseline Figure 5. Proportion of primary, secondary and tertiary genetic assignment of BWT steelhead transported upstream of Swift Dam from the Merwin Trap in 2014 (n=35). 2.5 Spawning and Egg Take A total of 26 females were spawned with 25 males through 26 spawning crosses (Appendix D). All fish were spawned during 26 spawning events between April 7 and May 16, 2014 (Figure 6). The target goal of 25 females and 25 males was achieved for the first time since 2009 (Table 4) also represents the largest number of pairwise crosses since the program began. However, the spawning period was very short (39 days), which is second only to 2013 in which there were only 8 total crosses. Fecundity averaged 3,849 per female. Estimated egg take (106,000) was more than the program target of 80,000 ± 20%. However, two crosses resulting in about 9,000 eggs were separated after primary assignment probabilities of the parents were within the Cascade Stratum. Eggs from these crosses were hatched and released as unfed fry into the North Fork 9

14 Lewis River. Appendix C provides the spawning log for 2014 indicating fecundity, crosses, eyed egg take and estimated egg loss. 6 5 Males Females Number of Spawners April 7 April 11 April 15 April 21 April 24 April 25 April 29 May 2 May 9 May 16 Spawn Date Figure 6. Number and timing of winter steelhead (males and females) spawned at Merwin hatchery (n=51) in Table 4. Number of spawning crosses and parents including the duration of each spawning periods for brood years between 2009 and Brood Year Crosses Females Males Spawn Period Days Mar 2 May Mar 17 May Mar 30 May Apr 10 May Apr 10 May Apr 7 May

15 2.6 Rearing, Tagging and Release Of the 82,492 fry ponded, 11 percent (8,954) succumbed to natural mortality. Mortality caused by bacterial coldwater disease (BCW) was low at about 2 percent. Flexibacter columnaris was not observed during the rearing cycle. Past F. columnaris infections have caused significant mortality (up to 50%). Because the egg take goal was achieved and pond loss was low, the 2015 release is expected to exceed all previous releases and exceed release targets by 21,000 smolts Rearing Table 5. Summary of rearing statistics for the 2014 brood year GENERAL STATISTIC Egg Take 106,038 Eyed Eggs 93,174 Total Fry Ponded 82,492 Total Fry out planted (unfed) 9,092 MORTALITY Egg Loss 6.83% Total Pond Loss 10,903 Due to natural causes 8,954 Due to disease (Columnaris) 0 Due to disease (BCW) 1,949 Overall Loss (egg and pond) 17,734 Shortage / Adjustment 1,911 RELEASE Projected Smolt Release (survival) 71,200 Release Date (Start Volitional) May 1, 2015 Release size 8 fpp 11

16 Number of Juvenile Steelhead May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar Apr Month Figure 7. Observed mortality rate of fish ponded for brood years 2009 through Tagging All subyearling steelhead were tagged with blank wire snout tag in December In addition, a small portion (about 1,200) of the release will receive a PIT tag in the dorsal sinus and reared in circular tanks for approximately 6 to 8 weeks prior to release. This effort is a pilot study to evaluate survival of circular tank reared fish versus traditional raceway or ponding strategies Release All fish will be volitionally released on May 1, 2015 at the Merwin boat launch. Volitional release will continue until June 1, Any fish remaining in the ponds on June 1, 2015 will be 12

17 forced out and released downstream of the County Bridge in Woodland, WA. Projected average release size in 8 per pound. A total release number of 71,200 smolts is projected. 3.0 MONITORING AND EVALUATION 3.1 Winter Steelhead Redd Surveys (Lower River) Redd surveys are used to estimate spawning abundance and distribution of winter steelhead in the mainstem North Fork Lewis River. Surveys are conducted weekly throughout the spawning period, which typically starts March 1 and extends into mid June Spawning Abundance Spawning abundance estimates rely on new redd census data, assumed sex ratio and females per redd to calculate total spawner abundance (Freymond and Foley 1986). Females per redd follow WDFW generalized guidelines of 0.81 females per redd and sex ratio is assumed equal (Table 6). To increase precision in 2013 and 2014, the equal sex ratio assumption is compared to the observed sex ratio at the MCF. This may be a more accurate estimate of female to male ratio in the river because of the large numbers captured in the trap and is unbiased in terms of capture efficiency (Table 6). Using trap data collected for 2014, a total of 1074 steelhead were trapped. These include mainly BWT steelhead, but also include steelhead held for broodstock and steelhead released because of stubby dorsal fins and no wire tag in their snout. Of this total, 476 were male and 598 were female. Therefore, for every female we assume that there are 0.80 males (instead of 1) that make up the population. As a result, the population estimate is lower than assuming the 1:1 ratio (Table 6). The number of redds per female should also be verified at some point to improve the accuracy of this estimate. Table 6. Wild Winter Steelhead Abundance Downstream of Merwin Dam 2008 through 2014 based on redd counts. Number of Redds observed Year Spawner Observed sex ratio Estimate (females : males) : : Spawner Estimate (Corrected) 13

18 Spawner Estimate Spawner Estimate (Corrected) Number of Spawners Year Figure 8. Wild winter steelhead spawner abundance downstream of Merwin Dam 2008 through 2014 based on redd counts Distribution A summary of redd distribution is provided in Table 7. Reach number 5 has the most redds, but also has the most habitat at 7.71 miles (not including the section downstream of Eagle Island). Redd density is highest in reach number 2 at 127 redds per mile. The relatively large number of redds in this reach is attributable to a small but heavily used spawning area approximately 0.75 miles downstream of Merwin Dam (Haggies). It is not fully understood why this area is used so extensively used by steelhead for spawning but it remains consistent from year to year. One key difference in this area is the gravel size (relatively small) and consistent flow rates along the tailout of the main pool. Redd locations are illustrated in Appendix E. Reach breaks coincide with WDFW reach breaks used for fall Chinook sampling. 14

19 Table 7. Redd distribution summary for reaches 1 through 5 including redds per mile Reach Reach Length (miles) Redds Redd per Mile Total number of redds = 364 Average redds per mile = 33 (69 in 2013) 3.2 Double Floy tagged steelhead returns to the Merwin Trap PacifiCorp biologist double floy tagged captured blank wire tagged steelhead from tangle netting and then released them back to river. Only 14 BWT steelhead were tagged with double floy tags and released in This low number was a result of low capture rates and a majority of the fish being ripe when caught. Consequently, no double floy fish were recaptured at the MCF. One double floy tagged fish was, however, recaptured in the tangle net. This fish was first captured at Haggies on March 25, 2014 and later recaptured in the same location on April 15, The 670 mm male had lost one of the floy tags and was ripe when recaptured. It is assumed that this male spawned at Haggies. Given the low tagging and recapture rate it is not possible to make conclusions regarding recruitment to the trap or presence of BWT steelhead on the spawning grounds using this method. 3.3 Ratio of NOR to HOR steelhead in the traps and tangle netting Program returns are treated as hatchery origin (HOR) steelhead despite their genotype assignment to NOR stocks. This is due to the hatchery influence during mating and captive rearing conditions during their first year of life. As these program fish return as adults, there is opportunity for these (HOR) fish to spawn with NOR stocks. It has been shown that reproductive success (fitness) declines rapidly (up to 37 percent per captive reared generation) within a natural population (Araki et. al. 2007). The evolutionary mechanisms for declines in fitness are not fully understood, but hatchery protected rearing environments and controlled mating selection are suspected contributors to this decline (Araki et. al 2007). Inbreeding between program fish is also a concern because of loss in genetic diversity or effective population size further limits fitness and adaptability of the natural spawning population. In 2014, the ratio of HOR to NOR captured through tangle netting is used to estimate the potential influence of HOR on the NOR population. Tangle netting provides a direct measure of the proportion of hatchery to natural origin fish on the spawning grounds when done during 15

20 spawning and in areas where spawning is occurring. Figure 1 and 2 provides the total number of HOR and NOR s captured in both the MCF and through tangle netting. The MCF, however, should not be used to estimate phos as it is biased towards BWT steelhead because Merwin hatchery effluent water is used to supplement attraction water for the trap. To estimate phos through tangle netting, the number of HOR (includes BWT) and NOR are recorded during the natural spawn time of NOR winter steelhead in the Lewis River (March June). The total HOR and NOR captures during this period provides an estimate of the ratio present on the spawning grounds (Table 8). The use of tangle netting is limited by its duration in that netting normally does not extend past the first or second week of May. Netting past this date raises the potential for disrupting and perhaps reducing natural spawning success, because nearly all steelhead captured past this date are either kelts or active spawners. Despite this limitation, tangle netting remains the only means at this time to directly sample and determine origin of spawning steelhead for estimating phos. Table 8. Ratio of HOR and NOR steelhead captured in the tangle net from March 1 to May 12, 2014 (n=148). Total HOR 43* Total NOR 105 phos 29 % phos (excluding BWT) 3 % Males Females HOR NOR HOR NOR * Total includes 40 BWT and 3 AD clipped winter steelhead Based on tangle net captures during the spawning period, we estimate the proportion of hatchery origin spawners is 29 percent. The main contributor to this estimate is the number of BWT steelhead on the spawning grounds during the natural spawning period. If BWT steelhead are excluded and only AD clipped steelhead are included, phos is reduced 26 percent to only 3 percent, which exceeds HSRG targets for integrated programs. The ability to remove a meaningful number of BWT and AD clipped winter steelhead spawning in the lower river would be challenging without directly and irreversibly reducing NOR spawning success. 16

21 3.4 Upstream Transport of Winter Steelhead In 2014, a total of 1033 blank wire tagged steelhead were transported upstream of Swift Dam (Table 9). A total of 82 transported steelhead also received a surgically implanted radio transmitter for distribution studies in the upper basin. Distribution and detection data along biological data each of these fish is provided in the Aquatic Monitoring and Evaluation Report as an appendix to the ACC/TCC annual report. This year represents the third year of transportation activities and numbers continue to increase each year. The goal of the H&S program is 500 winter steelhead transported each year. We have exceeded this target the last two years and more than doubled the target in 2014 (Table 9). Table 9. Summary of Winter Steelhead transported and released upstream of Swift Dam. YEAR Number Number fitted with radio Transported transmitters Coho and Spring Chinook Salmon Redd Surveys Coho and Spring Chinook Salmon 2013: Meridian to provide Coho and Spring Chinook Salmon 2014: Meridian to provide Screw trap Operations As in 2013, the 2014 screw trapping effort was conducted near the golf course using one 8 foot diameter screw trap from April 1 through June 11, In total, 23,279 fish were captured (3,011 smolts) in the screw trap during the collection period (Table 10). Of these, 2,828 smolts were marked and transported upstream for recapture and trap efficiency testing (Table 11). Only 29 recaptures were reported from the marked release groups. Despite improvements in capture efficiency in 2014 (1.03%) as compared to 2013 (0.60%), trapping efficiency remains low for calculating abundance with acceptable confidence. To help compensate for low trapping efficiencies, the trapping crew marked and released all smolts captured in the screw trap (2,828) upstream for efficiency testing. 17

22 Meridian to provide analysis Table 10. Collection summary for the rotary screw trap deployed downstream of Merwin Dam April 1 June 11, Species Life Stage No Collected Period of collection Mean Length (FL, mm) MIN (mm) MAX (mm) σ Coho Rainbow Chinook Cutthroat Chum Lamprey TOTAL Table 11. Disposition of captured fish (excluding fry) including catch rates of BWT and non BWT rainbow form April 1 June 11, Total Released upstream Rainbow Coho Cutthroat Recaptures 29 (1.03%) Total Released Downstream Rainbow Cutthroat Coho Non Clipped Rainbow BWT Non BWT 18

23 3.7 Mainstem Carcass Surveys Coho and Spring Chinook Salmon *NOTE: Mainstem and tributary carcass survey data for spring Chinook and coho are surveyed beginning in the fall season of each year and extend until the end of January. This schedule does not typically provide adequate time to input, review and analyze collected data and present the results in formal reporting by April 1 of each year. Therefore, reporting for mainstem and tributary surveys of coho and spring Chinook are delayed one year from the survey period. For example, survey data collected in the fall of 2013 (and early 2014) are not reported until the 2014 annual operations report is finalized and submitted to the FERC in April of There may also be some instances where more information is needed before final results are presented which further delays reporting. However, placeholders titles in each report iteration are never removed until the data are available, analyzed or deemed inadequate Coho Salmon Tributary Surveys 2012: WDFW to provide results Coho Salmon Surveys 2013: Meridian to provide results Spring Chinook Surveys 2013: Meridian to provide results Coho Salmon Surveys 2014: Meridian to provide results Spring Chinook Surveys 2014: Meridian to provide results 4.0 RECOMMENDATIONS FOR ONGOING MANAGEMENT Improvements in the way we handle and rear steelhead from wild winter broodstock continues to improve. Noticeable declines in mortality of both adults and rearing juveniles has declined from previous years. Also, rearing conditions have improved as recommendations from the H&S subgroup have been implemented. The pilot test use of circular tanks (as opposed to ponds) will continue for the 2014 brood year to determine if survival improves as is supported by several studies. If results confirm benefits to survival (SAR) the use of circular tank rearing may be expanded to include a larger portion of the smolt releases. Developing estimates of abundance for juveniles that meet precision goals continues to be a challenge. Annual planning meetings need to focus on developing standardized sampling 19

24 methods that strives to meet NOAA precision guidelines for monitoring fish populations. Our trapping efforts for estimating juvenile abundance currently does not achieve collection efficiencies needed to calculate precise estimates of abundance. In 2015, the placement of an additional trap may improve trap efficiency to a level that allows more precise estimators of abundance. In addition to monitoring methods, the H&S Subgroup should focus on the effects of the late winter broodstock program on NOR winter steelhead stocks in the lower river. The effects of mining and program returns need closer evaluation. While the program is temporary, there may be long term genetic impacts to the locally adapted population that may not yet be realized. These potential impacts should be monitored with methods that are now available to quantify metrics such as effective population size and inbreeding coefficients. These metrics are consistent with regional best management practices and should be developed further for the North Fork Lewis River late winter steelhead program. 5.0 REFERENCES Araki, H., Cooper, B., and S. Blouin Genetic Effects of Captive Breeding Cause a Rapid, Cumulative Fitness Decline in the Wild. Science, vol. 318, Octber 5, Freymond, B., and S. Foley Wild steelhead spawning escapement estimates from Boldt Case rivers Washington Department of Game. Fish Management Division. Rep. No Olympia,WA. Hilborn, R., B. G. Bue, and S. Sharr Estimating spawning escapement for periodic counts: a comparison of methods. Canadian Journal of Fisheries and Aquatic Sciences 56: Hill, R. A Optimizing aerial count frequency for area under the curve method of estimating escapement. North American Journal of Fisheries Management 17: Hood, G. M Pop Tools version URL Kinsel, C., P. Hanratty, M. Zimmerman, B. Glaser, S. Gray, T. Hillson, D. Rawding, and S. VanderPloeg Intensively Monitored Watersheds: 2008 Fish Population Studies in the Hood Canal and Lower Columbia Stream Complexes. FPA 09 12, Washington Department of Fish and Wildlife, Olympia, Washington. 20

25 Appendix A Wild Winter Steelhead Collection Log 2014

26 CAPTURE DATE CAPTURE METHOD Gender Fork Length (cm) Pit Tag # DNA Sample # Genetic Probability Assignment Comments 2/21/2014 Tangle Net M 72 A0A0 TN14 1 LRMerwin /25/2014 Tangle Net M 93 A05E TN14 2 LRMerwin /4/2014 Cedar F 76 3D91C2DD5601E CC14 14 LRMerwin /4/2014 Cedar M 68 3D91C2DD678B CC14 20 LRMerwin /4/2014 Cedar M 71 3D91C2DD51559 CC 21 LRCedar.7514 Mortality 3/28/14 3/11/2014 Tangle Net M 78 0EE0 TN14 3 LRMerwin /11/2014 Tangle Net F 80 0F1A TN14 4 LRMerwin /11/2014 Tangle Net M 74 0F1F TN14 5 LRMerwin /11/2014 Tangle Net M 82 0E87 TN14 6 LRMerwin /11/2014 Tangle Net M 87 0E5F TN14 7 LRMerwin.9644 To Many Males / Returned to river Tangle Net 3/11/2014 Tangle Net M 61 0F28 TN14 8 LRMerwin.8317 To Many Males / Returned to river Tangle Net 3/11/2014 Tangle Net M 68 A086 TN14 9 LRMerwin.7792 To Many Males / Returned to river Tangle Net 3/11/2014 Tangle Net M 71 9E8D TN14 10 LRMerwin.9484 To Many Males / Returned to river Tangle Net 3/17/2014 Tangle Net M 78 A07F TN14 11 LRCedar /17/2014 Tangle Net F 71 A0A3 TN14 12 LRCedar /23/2014 Merwin Trap F 73 A20D MT14 1 LRMerwin /25/2014 Tangle Net F 69 A0B3 TN14 13 LRCedar /25/2014 Tangle Net M 95 A092 TN14 14 LRCedar /25/2014 Tangle Net F 66 A068 TN14 15 LRMerwin /25/2014 Tangle Net M 71 A057 TN14 16 LRCedar /26/2014 Merwin Trap F 71 A1FB MT14 2 LRMerwin.909 3/27/2014 Merwin Trap M 91 A226 MT14 3 LRMerwin /1/2014 Merwin Trap M 65 A1FA MT14 4 LRCedar /1/2014 Tangle Net F 63 0F08 TN LRMerwin /1/2014 Tangle Net F 79 0F45 TN LRMerwin /2/2014 Merwin Trap M 81 A22C MT 14 5 LRMerwin /4/2014 Tangle Net M 67 0E40 TN LRMerwin /4/2014 Tangle Net M 74 0F30 TN LRCedar.019 4/4/2014 Tangle Net M 96 0EC9 TN LRMerwin /4/2014 Tangle Net M 79 0E9C TN LRMerwin /8/2014 Tangle Net F 67 0E42 TN LRMerwin.5953 Genetic sample mixed w/ 264 4/8/2014 Tangle Net F 73 0E91 TN LRCedar.9081 Genetic sample mixed w/ 265 4/8/2014 Tangle Net F 63 0EFD TN LRCedar.5404 Genetic sample mixed w/ 266 4/8/2014 Tangle Net F 72 0EAD TN LRMerwin /8/2014 Tangle Net F 72 0EF9 TN LRMerwin /8/2014 Tangle Net M 80 0E99 TN LRMerwin.985 4/8/2014 Tangle Net F 71 0ED8 TN LR Cedar.4531 Skipped Red vials /21/2014 Merwin Trap F MT 14 6 Mill Cr URT Unfed fry plant 4/22/2014 Tangle Net F 77 0F04 TN LRMerwin.7704 APPENDIX A 1

27 CAPTURE DATE CAPTURE METHOD Gender Fork Length (cm) Pit Tag # DNA Sample # Genetic Probability Assignment 4/22/2014 Tangle Net F 74 0E90 TN LRMerwin /22/2014 Tangle Net M 64 0EDF TN LRMerwin /24/2014 Merwin Trap F 71 A246 MT 14 7 LRCedar /24/2014 Merwin Trap F 67 A1FD MT 14 8 LRCedar /25/2014 Merwin Trap M 95 A243 MT 14 9 LRMerwin /25/2014 Tangle Net F 81 0E8A TN LRMerwin /25/2014 Tangle Net M 61 0F4A TN LRCedar.8177 Spawned Out 4/25/2014 Tangle Net M 77 0F2B TN LRMerwin /29/2014 Tangle Net M 73 0EE4 TN LRMerwin /29/2014 Tangle Net M 75 0EEE TN ElochHat /29/2014 Tangle Net M 72 6A36 TN LRMerwin /29/2014 Tangle Net M 89 6A44 TN LRMerwin.8872 MORTALITY 4/30 4/29/2014 Tangle Net F 66 6A4F TN EFLewis /29/2014 Tangle Net M 71 6A46 TN LRCedar /29/2014 Tangle Net F 76 6A23 TN LRMerwin /29/2014 Tangle Net M 79 6A24 TN SandyR /29/2014 Tangle Net M 47 6A34 TN LRMerwin /2/2014 Merwin Trap F 83 A235 MT Grays R..498 Unfed Fry Plant Comments 5/3/2014 Merwin Trap M 76 A239 MT LRMerwin.496 5/6/2014 Merwin Trap M 77 A23E MT LRCedar /6/2014 Merwin Trap F 70 A213 MT LRMerwin /6/2014 Merwin Trap M 86 A229 MT LRMerwin.9826 Spawned Out 5/6/2014 Merwin Trap M 86 A1F7 MT LRMerwin /6/2014 Tangle Net M 89 6A31 TN LRMerwin /6/2014 Tangle Net M 74 6A45 TN GraysR /6/2014 Tangle Net F 75 6A70 TN LRMerwin /6/2014 Tangle Net F 71 6A6E TN LRMerwin.763 5/7/2014 Merwin Trap F 73 A208 MT LRMerwin /8/2014 Merwin Trap M 75 A21F MT LRMerwin /8/2014 Merwin Trap F 74 A216 MT LRMerwin /12/2014 Merwin Trap F 70 A207 MT LRMerwin.235 no assigned males on hand 5/12/2014 Merwin Trap F 72 A1EB MT LRMerwin.8029 no assigned males on hand 5/12/2014 Merwin Trap F 67 A234 MT LRMerwin /12/2014 Tangle Net M 77 6A65 TN LRCedar.567 5/12/2014 Tangle Net M 73 6A22 TN LRCedar /14/2014 Lewis M 77 A20A MT Lewis River Ladder APPENDIX A 2

28 APPENDIX B: Genetic Assignment Results from Lewis River Captures at Merwin Trap (MT), Cedar Creek (CC) and Tangle Netting (TN) 2014

29 DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 03/07/14 cc14 01 LRMerwin LRCedar KalamSu KalamW GarysR /07/14 cc14 02 GermCr MillCr KalamW SFTout ElochHat /07/14 cc14 03 MillCr GermCr GarysR /07/14 cc14 04 LRCedar LRhatW LRMerwin ElochHat /07/14 cc14 06 KlickSu MillCr Cowman ElochHat LRhatW /07/14 cc14 07 SFTout LRCedar LRMerwin GrRLC KalamW /07/14 cc14 08 GermCr LRCedar LRMerwin KalamSu GarysR /07/14 cc14 09 LRCedar KalamSu LRhatW GermCr GrRLC /07/14 cc14 10 LRCedar MillCr GermCr LRMerwin SFTout /07/14 cc14 11 LRMerwin LRCedar ElochHat /07/14 cc14 12 GarysR LRCedar LRMerwin Cowman GrRLC /07/14 cc14 13 EFLewisR LRCedar Cowman GermCr KalamW /07/14 cc14 14 SandyR GarysR LRMerwin Cowman GermCr /07/14 cc14 15 LRCedar ElochHat Cowman GermCr /07/14 cc14 16 LRhatW LRCedar LRMerwin EFLewisR Cowman /07/14 cc14 17 LRCedar ElochHat KalamW LRMerwin Cowman /07/14 cc14 18 Cowman LRMerwin LRCedar /07/14 cc14 19 GarysR LRhatW GermCr Cowman LRCedar /07/14 cc14 20 LRMerwin LRCedar Cowman KalamW KalamSu /07/14 cc14 21 LRCedar LRMerwin MillCr GarysR Cowman /31/14 CC14 22 LRCedar Cowman LRMerwin SandyR ElochR /31/14 CC14 23 LRMerwin HoodR LRCedar Cowman /31/14 CC14 24 LRCedar ElochHat /31/14 CC14 25 LRCedar Cowman LRhatW GrRLC KalamW /31/14 CC14 26 Cowman MillCr LRCedar LRMerwin ElochHat /31/14 CC14 27 Cowman LRCedar LRMerwin /31/14 CC14 28 LRMerwin LRCedar KalamW MillCr Cowman /31/14 CC14 29 LRMerwin /31/14 CC14 30 LRCedar GermCr LRMerwin GrRLC Cowman /21/14 cc14 31 LRMerwin LRCedar GermCr Cowman /21/14 cc14 32 LRCedar LRMerwin Cowman LRhatW APPENDIX B 1

30 DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 05/21/14 cc14 33 KalamW GermCr SandyR GarysR EFLewisR /21/14 cc1434 LRCedar MillCr GermCr LRMerwin Cowman /21/14 cc1435 BigCr LRMerwin GermCr LRCedar MillCr /21/14 cc1436 LRMerwin LRCedar KalamW /21/14 cc1437 LRCedar EFLewisR KalamW 0.01 LRMerwin /21/14 cc1438 LRMerwin LRCedar KalamW /21/14 cc1439 LRMerwin LRCedar Cowman GrRLC GarysR /21/14 cc14 40 LRCedar LRMerwin GermCr SandyR GrRLC /21/14 cc14 41 LRMerwin Cowman GermCr LRCedar MillCr /21/14 cc14 42 LRCedar MillCr GermCr LRMerwin KalamW /21/14 cc14 43 GermCr GrRLC LRCedar GarysR BigCr /21/14 cc14 44 LRCedar LRMerwin Cowman KalamW LRhatW /21/14 cc14 45 LRMerwin /21/14 cc14 46 LRMerwin GermCr /21/14 CC14 47 LRCedar MillCr LRMerwin GermCr Cowman /16/14 CC14 48 MillCr KalamW KalamSu GermCr LRCedar /21/14 CC14 48 KalamW MillCr GermCr KalamSu LRCedar /16/14 CC14 49 LRCedar LRMerwin /21/14 CC14 49 LRCedar LRMerwin /16/14 CC14 50 SkamHat GermCr KalamSu LRMerwin EFLewisR /21/14 CC14 50 SkamHat GermCr KalamSu LRMerwin EFLewisR /16/14 CC14 51 LRCedar LRMerwin Cowman GarysR MillCr /21/14 CC14 51 LRCedar LRMerwin Cowman GarysR MillCr /02/14 MT14 07 LRCedar LRMerwin /02/14 MT14 08 LRCedar LRMerwin GarysR SandyR /02/14 MT14 09 LRMerwin LRCedar Cowman EFLewisR /31/14 MT14 1 GrRLC LRMerwin LRCedar MillCr ElochR /09/14 MT14 10 GarysR LRCedar GermCr SandyR KalamSu /09/14 MT14 11 LRMerwin LRCedar GarysR GermCr /09/14 MT14 12 LRCedar LRMerwin Cowman /09/14 MT14 13 LRMerwin Cowman LRCedar APPENDIX B 2

31 DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 05/09/14 MT14 14 LRMerwin GrRLC /09/14 MT14 15 LRMerwin LRCedar /09/14 MT14 15 LRMerwin /16/14 MT14 16 LRMerwin /16/14 MT14 17 LRMerwin LRCedar KalamW /16/14 MT14 18 LRMerwin LRCedar KalamW Cowman EFLewisR /16/14 MT14 19 GermCr LRMerwin KalamW Cowman ElochHat /05/14 MT14 2 LRMerwin LRCedar /16/14 MT14 20 LRMerwin LRCedar Cowman ElochR GarysR /16/14 MT14 21 LRMerwin LRCedar /05/14 MT14 3 LRMerwin LRCedar Cowman /05/14 MT14 4 LRCedar GrRLC KalamW Cowman /11/14 MT14 5 LRMerwin LRCedar KalamW Cowman GrRLC /16/14 TN13 32 Cowman LRCedar LRMerwin /16/14 TN13 33 LRMerwin GermCr LRCedar GarysR /16/14 TN13 34 LRCedar LRMerwin Cowman KalamW /16/14 TN13 35 LRCedar GarysR Cowman ElochR LRMerwin /16/14 TN13 36 LRMerwin LRCedar /16/14 TN13 37 LRCedar LRMerwin KalamW KalamSu GermCr /16/14 TN13 38 LRMerwin LRCedar /16/14 TN13 39 LRCedar LRMerwin EFLewisR GermCr Cowman /16/14 TN13 40 LRMerwin GermCr /16/14 TN13 41 LRCedar LRMerwin KalamW KalamSu GarysR /16/14 TN13 42 LRMerwin Cowman GermCr LRCedar GrRLC /16/14 TN13 43 LRMerwin LRCedar EFLewisR KalamW /02/14 TN13 61 LRCedar GermCr EFLewisR GrRLC KalamW /02/14 TN13 62 LRMerwin GermCr LRCedar /02/14 TN13 63 LRMerwin LRCedar Clack /02/14 TN13 64 LRCedar ElochHat LRMerwin GermCr Cowman /02/14 TN13 65 LRMerwin LRCedar Cowman EFLewisR /02/14 TN13 66 LRMerwin LRCedar SandyR EFLewisR GermCr APPENDIX B 3

32 DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 05/02/14 TN13 67 EFLewisR KalamSu LRMerwin SandyR GermCr /02/14 TN13 68 LRCedar LRMerwin Cowman GermCr GrRLC /02/14 TN13 69 LRMerwin LRCedar /02/14 TN13 70 SandyR LRMerwin GrRLC LRCedar /02/14 TN13 71 LRMerwin LRCedar /09/14 TN13 72 LRMerwin LRCedar GrRLC KalamSu KalamW /09/14 TN13 73 LRMerwin LRCedar GermCr KalamW /09/14 TN13 74 GarysR GrRLC GermCr MillCr LRMerwin /09/14 TN13 75 LRCedar GarysR LRMerwin KalamSu /09/14 TN13 76 LRMerwin /09/14 TN13 77 LRMerwin LRCedar GrRLC /09/14 TN13 78 LRMerwin LRCedar /21/14 TN14 1 LRMerwin GrRLC* KalamW Cowman KalamSu /25/14 TN14 2 LRMerwin LRCedar /14/14 TN14 3 KalamSu LRMerwin EFLewisR GrRLC KalamW /14/14 TN14 4 LRMerwin LRCedar GermCr KalamW GarysR /14/14 TN14 5 LRCedar GermCr LRMerwin KalamW GarysR /14/14 TN14 6 LRMerwin LRCedar KalamW Cowman /14/14 TN14 7 LRMerwin LRCedar /14/14 TN14 8 LRMerwin KalamW GermCr /14/14 TN14 9 LRMerwin LRCedar SandyR EFLewisR GermCr /14/14 TN14 10 LRMerwin LRCedar /20/14 TN14 11 LRCedar LRMerwin KalamW GarysR Cowman /20/14 TN14 12 SFTout LRCedar Cowman KalamW SandyR /31/14 TN14 13 LRCedar LRMerwin Cowman KalamSu GermCr /31/14 TN14 14 LRCedar MillCr SandyR GermCr ElochR /31/14 TN14 15 LRMerwin /31/14 TN14 16 GermCr LRCedar KalamSu EFLewisR SandyR /05/14 TN14 17 LRMerwin LRCedar GermCr Cowman /05/14 TN14 18 LRMerwin KalamW GrRLC /11/14 TN14 19 LRMerwin LRCedar Cowman APPENDIX B 4

33 DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 04/11/14 TN14 20 GermCr ElochR GarysR LRCedar MillCr /11/14 TN14 21 LRMerwin /11/14 TN14 22 LRMerwin LRCedar GermCr EFLewisR Cowman /11/14 TN14 23 LRMerwin /11/14 TN14 24 LRMerwin Cowman KalamSu LRCedar /11/14 TN14 25 LRCedar LRMerwin Cowman /11/14 TN14 26 LRMerwin LRCedar LRhatW ElochHat Cowman /11/14 TN14 27 LRMerwin LRCedar GrRLC 0.03 KalamW /11/14 TN14 28 LRMerwin LRCedar /11/14 TN14 29 LRMerwin GermCr LRCedar SandyR ElochR /11/14 TN14 30 LRCedar LRMerwin GermCr Cowman ElochHat /11/14 TN14 31 LRCedar LRMerwin GermCr KalamW EFLewisR /11/14 TN14 32 LRMerwin LRCedar /18/14 TN14 33 LRMerwin LRCedar Cowman /18/14 TN14 34 LRCedar Cowman LRMerwin ElochHat /18/14 TN14 35 LRMerwin LRCedar Cowman KalamSu /18/14 TN14 36 GermCr KalmFHat Cowman LRMerwin LRCedar /18/14 TN14 38 LRMerwin GrRLC LRCedar KalamW /18/14 TN14 39 LRCedar KalamW GarysR LRMerwin KalamSu /18/14 TN14 40 LRMerwin LRCedar GermCr KalamW EFLewisR /18/14 TN14 41 LRMerwin LRCedar KalamW Cowman /18/14 TN14 42 LRCedar ElochHat LRMerwin /18/14 TN14 43 LRMerwin GarysR /18/14 TN14 44 LRMerwin LRCedar GermCr SandyR /18/14 TN14 45 LRMerwin LRCedar ElochR GarysR GermCr /18/14 TN14 46 LRCedar LRMerwin /18/14 TN14 47 LRCedar LRMerwin ElochHat /25/14 TN14 48 LRMerwin LRCedar /02/14 TN14 49 EFLewisR GermCr LRMerwin Cowman LRCedar /25/14 TN14 50 LRMerwin LRCedar GermCr Cowman /25/14 TN14 51 LRMerwin MillCr LRCedar LRhatW GermCr APPENDIX B 5

34 DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 04/25/14 TN14 52 LRCedar KalamSu SandyR LRMerwin EFLewisR /25/14 TN14 53 LRMerwin GermCr KalamW MillCr /25/14 TN14 54 LRMerwin LRCedar /25/14 TN14 56 LRMerwin LRCedar GarysR /25/14 TN14 57 LRCedar Cowman LRMerwin HoodR LRhatW /25/14 TN14 58 LRMerwin /25/14 TN14 59 LRMerwin LRCedar MillCr /02/14 TN14 60 LRMerwin LRCedar GermCr /20/14 URT LRMerwin LRCedar SFTout /20/14 URT LRCedar 0.7 LRMerwin KalamW /20/14 URT LRCedar LRMerwin Cowman GermCr KalamW /31/14 URT LRCedar GermCr LRMerwin KlickSu /31/14 URT LRMerwin LRCedar LRhatW GrRLC ElochR /31/14 URT LRMerwin LRCedar LRhatW /05/14 URT LRCedar GarysR LRMerwin GrRLC KalamW /05/14 URT LRMerwin LRCedar EFLewisR GarysR ElochR /05/14 URT LRCedar GermCr LRMerwin EFLewisR GarysR /05/14 URT LRMerwin LRCedar ElochR GermCr MillCr /05/14 URT LRMerwin Cowman LRCedar KalamW ElochR /05/14 URT LRMerwin 0.76 LRCedar /05/14 URT LRCedar LRMerwin GarysR ElochHat MillCr /05/14 URT LRMerwin EFLewisR MillCr GrRLC SandyR /05/14 URT LRCedar LRMerwin /11/14 URT LRMerwin GrRLC GermCr LRCedar Cowman /11/14 URT LRMerwin LRCedar MillCr Cowman /11/14 URT LRCedar LRMerwin GermCr Cowman KalamW /18/14 URT LRMerwin LRCedar Cowman /18/14 URT LRMerwin LRCedar LRhatW ElochHat Cowman /18/14 URT LRMerwin LRCedar ElochR GarysR GermCr /18/14 URT LRMerwin LRCedar /25/14 URT LRCedar GermCr ElochHat LRhatW GarysR APPENDIX B 6

35 DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 04/25/14 URT MillCr Cowman LRMerwin LRCedar KalamSu /25/14 URT LRMerwin LRCedar Cowman /25/14 URT LRCedar GrRLC LRMerwin GermCr KalamSu /02/14 URT LRMerwin GermCr Clack GarysR /02/14 URT LRMerwin LRCedar EFLewisR Cowman /02/14 URT LRCedar LRMerwin GermCr Cowman ElochHat /02/14 URT LRCedar LRMerwin Cowman /02/14 URT Clack LRCedar LRMerwin Cowman /02/14 URT LRMerwin LRCedar Cowman /02/14 URT LRMerwin LRCedar /14/14 URT 66 LRMerwin LRCedar /14/14 URT 77 LRCedar GermCr GarysR GrRLC ElochR APPENDIX B 7

36 Appendix C Wild Winter Steelhead Spawning Log 2014

37 Spawn Date Total Egg Weight w/o ovarian Estimate Eggs/Female Eyed Egg Eggs/LB Eyed Eggs On-Hand Dead Eggs Actual Eggs/Female Percent of Egg Loss Estimate # Fry Ponded Comments 4/7/ % /7/ % /7/ % 2698 Ponded 6/6 4/11/ % /15/ % /21/ % 191 Added to TN /21/ % /21/ % Plant NF Lewis 4/21/ % /24/ % /25/ % /29/ % /29/ % /29/ % /2/ % /2/ % Plant NF Lewis 5/2/ % /2/ % /9/ % /9/ % 1318 Added to TN /9/ % /9/ % /9/ % /16/ % /16/ % /16/ % % APPENDIX C 1

38 APPENDIX D Wild Winter Steelhead Spawning Crosses 2014

39 SPAWN DATE CROSS NO. 4/7/ /7/ /7/2014 4/11/2014 4/15/2014 4/21/2014 4/21/2014 4/21/2014 4/21/2014 4/24/2014 4/25/2014 4/29/2014 4/29/ FEMALES (DNA No. and Assignment Probability) MALES (DNA No. and Assignment Probability) TN 14 4 TN 14 5 LRMerwin.6138 LRCedar.3559 LRMerwin.2145 MT 14 1 CC LRMerwin.3813 LRCedar.1043 LRMerwin.7812 TN TN 14 2 LRCedar.5232 LRMerwin.1899 LRMerwin.9721 TN TN LRCedar.9081 LRMerwin.9969 MT 14 2 TN LRMerwin.909 LRMerwin.7458 TN MT 14 5 LRMerwin.9928 LRMerwin.9088 TN TN 14 6 LRMerwin.9264 LRMerwin.6853 MT 14 6 TN Mill Cr LRMerwin.985 TN TN LRMerwin.7531 LRMerwin.4237 LRCedar.3772 MT 14 7 TN LRCedar.5262 LRMerwin.4588 LRCedar.4302 LRMerwin.3456 TN TN LRMerwin.8184 LRMerwin.5357 MT 14 3 TN MT 14 4 LRMerwin.9068 LRMerwin.9299 LRCedar.8996 TN LRMerwin LRCedar MT 14 3 MT 14 4 LRMerwin.9068 LRCedar.8996 APPENDIX D 1

40 SPAWN DATE 4/29/2014 5/2/2014 5/2/2014 5/2/2014 5/2/2014 5/9/2014 5/9/2014 5/9/2014 5/9/2014 5/9/2014 5/16/2014 5/16/2014 5/16/2014 CROSS NO TOTAL INDIVIDUALS FEMALES (DNA No. and Assignment Probability) MALES (DNA No. and Assignment Probability) TN 14 4 TN 14 5 TN MT 14 3 MT 14 4 LRMerwin.9488 LRMerwin.9068 LRCedar.8996 TN MT 14 9 LRMerwin.9712 LRMerwin.9349 MT TN Grays R..498 LRMerwin.6964 MT 14 7 TN LRCedar.6874 LRCedar.4853 LRMerwin.4728 TN TN LRCedar.5404 LRMerwin.8515 TN MT LRMerwin.7704 LRMerwin.496 LRCedar.2467 TN TN LRMerwin.5953 LRMerwin.9094 TN MT LRMerwin.6625 LRCedar.3059 LRMerwin.9817 TN TN LRMerwin.763 LRCedar LRMerwin LRCedar.2403 MT MT LRMerwin.6908 LRCedar LRCedar LRMerwin.4637 MT TN LRMerwin.6656 LRCedar.101 LRCedar.3651 LRMerwin.2634 MT TN LRMerwin.9945 LRCedar.567 LRMerwin.3762 MT MT LRMerwin.9341 LRCedar.0586 LRMerwin.9402 LRCedar APPENDIX D 2

41 Appendix E Steelhead Redd locations, Lewis River, WA 2014

42 Steelhead Redd Locations, Lewis River, WA 2014 Surveys Path: G:\work\common\MikeIchisaka\lewis\Lewis_Redds\Lewis_redds mxd 2/26/2015 Steelhead 2014 Source: Esri, DigitalGlobe, GeoEye, i-cubed, Earthstar Geographics, CNES/Airbus DS, USDA, USGS, AEX, Getmapping, Aerogrid, IGN, IGP, swisstopo, and the GIS User Community I Miles 1:13,000 WASHINGTON Map Sheet 1 of 3 Lewis River 3 2 Content may not reflec Map National Location Geog 1 MERWIN I

43 Steelhead Redd Locations, Lewis River, WA 2014 Surveys Path: G:\work\common\MikeIchisaka\lewis\Lewis_Redds\Lewis_redds mxd 2/26/2015 Steelhead 2014 Source: Esri, DigitalGlobe, GeoEye, i-cubed, Earthstar Geographics, CNES/Airbus DS, USDA, USGS, AEX, Getmapping, Aerogrid, IGN, IGP, swisstopo, and the GIS User Community I Miles 1:13,000 WASHINGTON Map Sheet 2 of 3 Lewis River 3 2 Content may not reflec Map National Location Geog 1 MERWIN I

44 Steelhead Redd Locations, Lewis River, WA 2014 Surveys Path: G:\work\common\MikeIchisaka\lewis\Lewis_Redds\Lewis_redds mxd 2/26/2015 Steelhead 2014 Source: Esri, DigitalGlobe, GeoEye, i-cubed, Earthstar Geographics, CNES/Airbus DS, USDA, USGS, AEX, Getmapping, Aerogrid, IGN, IGP, swisstopo, and the GIS User Community I Miles 1:13,000 WASHINGTON Map Sheet 3 of 3 Lewis River 3 2 Content may not reflec Map National Location Geog 1 MERWIN I