DNA barcoding and morphological identification of neotropical ichthyoplankton from the Upper Paraná and São Francisco
|
|
- Michael Newman
- 5 years ago
- Views:
Transcription
1 Journal of Fish Biology (2015) doi: /jfb.12707, available online at wileyonlinelibrary.com DNA barcoding and morphological identification of neotropical ichthyoplankton from the Upper Paraná and São Francisco R. A. Becker, N. G. Sales, G. M. Santos, G. B. Santos and D. C. Carvalho* Pontifícia Universidade Católica de Minas Gerais, Programa de Pós-graduação em Zoologia de Vertebrados, Laboratório de Genética da Conservação, Rua Dom José Gaspar, 500, Belo Horizonte, MG, Brazil (Received 6 October 2014, Accepted 1 April 2015) The identification of fish larvae from two neotropical hydrographic basins using traditional morphological taxonomy and DNA barcoding revealed no conflicting results between the morphological and barcode identification of larvae. A lower rate (25%) of correct morphological identification of eggs as belonging to migratory or non-migratory species was achieved. Accurate identification of ichthyoplankton by DNA barcoding is an important tool for fish reproductive behaviour studies, correct estimation of biodiversity by detecting eggs from rare species, as well as defining environmental and management strategies for fish conservation in the neotropics The Fisheries Society of the British Isles Key words: biodiversity; eggs; fishes; fresh water; larvae; molecular taxonomy. INTRODUCTION Unambiguous identification of fish eggs and larvae is an important tool for fish ecology and conservation. For instance, it may allow the detection of spawning areas, the monitoring of fish stocks affected by dams and improve fisheries management and conservation policies (Baumgartner et al., 2004; Reynalte-Tataje et al., 2004; Agostinho et al., 2007; Moura et al., 2008; Valdez-Moreno et al., 2010). The ontogenetic morphological variation of larvae and the extreme biological diversity of neotropical ichthyofauna (around 3500 described species) (Reis et al., 2003) means that studies related to egg and larva identification are restricted to a few well-studied areas (Nakatani et al., 2001). As a consequence of these issues, the neotropical region has the lowest number of studies related to ichthyoplankton identification, despite its species diversity (Oliveira et al., 2008; Hermes-Silva et al., 2009). Moreover, all published work to date regarding egg and larval identification has been based solely on morphological characteristics and rarely reached identification to the species level (Nakatani et al., 2001; Graça & Pavanelli, 2007). In some cases, the studies analysed only abundance of ichthyoplankton, avoiding any tentative taxonomic identification of larvae (Gogola et al., 2010). *Author to whom correspondence should be addressed. Tel.: +55 (31) ; The Fisheries Society of the British Isles
2 2 R. A. BECKER ET AL. Therefore, a major obstacle for a broader use of fish larvae and egg surveys is their unambiguous identification (Gleason & Burton, 2012). For instance, Ko et al. (2013) morphologically identified larvae from the Taiwan coast and reported that only 13 5% were identified to the species level and 43% could not be identified due to the lack of diagnostic morphological traits. On the other hand, using a DNA-based technique (i.e. DNA barcoding), 69% of all larvae were identified to the species level (Ko et al., 2013). Reported morphological difficulties are normally due to shared morphological features and intraspecific morphological variation (Bialetzki et al., 1998, 2001, 2008). Additionally, it is only possible to differentiate fish eggs as belonging to migratory or non-migratory species as the perivitellinic space is the only diagnostic characteristic available (Nakatani et al., 2001; Leite et al., 2007; Oliveira & Ferreira, 2008). A broad range of molecular tools has been used to improve ichthyoplankton identification accuracy, including species-specific fluorescent-labelled probes (Gleason & Burton, 2012) to a high-throughput robotic sample handling process (Richardson et al., 2007). Most studies make use of developed libraries for species identification through DNA barcoding (650 bp of the mitochondrial gene coi), a technique which allows the identification of samples lacking diagnostic morphological characteristics, such as processed fish products (e.g. fish fillets and caviar), stomach contents as well as fish larvae and eggs (Desalle & Amato, 2004; Carreon-Martinez et al., 2011; Carvalho et al., 2011a; Ko et al., 2013; Arroyave & Stiassny, 2014; Loh et al., 2014). For instance, Loh et al. (2014) developed and applied DNA barcoding to identify 14 of the 16 species inhabiting the Brisbane River (Australia), including a quantitative (q)pcr assay for species-specific detection and quantification. For marine fish species, results using DNA barcoding have shown a high error rate of morphological identification to the species level of eggs and larvae (Ahlstrom & Moser, 1976; Shao et al., 2002). When considering Brazilian biodiversity, with over 2587 known species representing around 25% of the described freshwater fish species (Buckup et al., 2007), however, the accuracy of morphological identification of ichthyoplankton has not yet been investigated. In order to investigate the gain achieved by integrating DNA barcoding and neotropical ichthyoplankton taxonomy, the accuracy of the morphological identification of larvae conducted by two independent laboratories was investigated and compared with identification provided by DNA barcodes. Secondly, the efficiency of morphological diagnostic feature for egg identification (i.e. perivitellinic space) was evaluated in differentiating migratory from non-migratory freshwater fish species. SAMPLING MATERIALS AND METHODS Ichthyoplankton samples were obtained from Nova Ponte reservoir, located on the Araguari River (upper Paraná River basin; S; W) and the Pará River (São Francisco Basin; S; W), south-eastern Brazil. The São Francisco River and the upper Paraná River Basin encompass the most urbanized and exploited area of Brazil, representing two major South American basins. Inventories of their freshwater fish fauna found at least 205 and 310 valid species in each of the rivers, respectively; however, many putative species are yet to be described (Alves et al., 2007; Langeaniet al., 2007). DNA barcode
3 DNA BARCODING OF NEOTROPICAL ICHTHYOPLANKTON 3 libraries have already been developed for most species from both fish faunas (Carvalho et al., 2011b; Pereira et al., 2013). A 500 μm conic net 1 45 m long and 38 cm in diameter was used for sampling. All ichthyoplankton samples were fixed in ethanol (100%) and measured (total length, L T ) using a stereoscopic microscope with a micrometre ruler coupled to the ocular lens. Before extracting DNA, the same larvae were morphologically identified by two independent research laboratories. As all eggs and larvae were macerated for DNA extraction, photographs were taken and kept as vouchers. Larvae and fish eggs are classified as zooplankton; therefore, no permit is needed for their collection in Brazil and no specific permission was required for collecting zooplankton from the sample sites. MORPHOLOGICAL IDENTIFICATION Eggs were identified as belonging to migratory species (eggs with a large perivitellinic space) and non-migratory (reduced perivitellinic space) (Nakatani et al., 2001; Leite et al., 2007; Oliveira & Ferreira, 2008) according to Agostinho et al. (2003). Larvae were identified to the lowest possible taxonomic level by two separate laboratories: the Conservation Genetics Laboratory at the Pontifícia Universidade Católica de Minas Gerais (Laboratory LGC-PUC), Brazil and the Ichthyoplankton Laboratory from the Núcleo de Pesquisas em Limnologia e Aquicultura (Laboratory NUPELIA), State University of Maringá, Brazil. The larvae s embryonic development was defined according to its ontogenetic development: larval yolk sac, preflexion, flexion and postflexion according to morphological traits suggested by Ahlstrom & Moser (1976) and Nakatani et al. (2001). Larvae belonging to the order Characiformes were identified by the absence of barbels, dermic plates, protractile mouth and the presence of an anal opening in the middle of the body. The order Gymnotiformes were characterized by the presence of the anal opening located on the anterior half of the body, a falciform-shaped body and the absence of a caudal fin. The order Siluriformes was differentiated by the presence of barbels. The Anostomidae has two small chromatophores on the frontal head region, and the family Characidae was identified by excluding the other characteristics that define other families from the Characiformes order. The family Poecilidae has highly developedeyesand a rounded caudalfin (Nakataniet al., 2001; Graça & Pavanelli, 2007). DNA BARCODING DNA was extracted using a modified salting-out protocol (Sunnucks & Hales, 1996) or commercial kits (Nucleo Spin kit and Nucleo Spin XS kit; Macherey-Nagel; following the manufacturer s instructions. Before DNA extraction, eggs and larvae were washed in ultrapure water and then macerated. Partial cytochrome c oxidase I (coi) gene sequences of c. 650 bp were amplified by PCR using primers FishF1 and FishR1 (Ward et al., 2009). PCR consisted of 1 0 μl of buffer, 0 2 μl of deoxynucleotide triphosphate (dntp) (10 mm), 0 3 μl of MgCl 2,0 2 μl of each primer (10 μm), 0 1 μl of Taq polymerase, 7 0 μl of ultrapure water and 1 0 μl of genomic DNA. PCR conditions comprised an initial step of 2 min at 95 Cfollowed by 35 cycles of 30 s at 95 C, 30 s at 54 C and 60 s at 72 C, and one final step of 10 min at 72 C. PCR products were visualized in 1% agarose gel, and successfully amplified samples were selected for sequencing. DNA sequencing was conducted in both directions, and sequences were obtained using an automated DNA sequencing device 3500 (Life technologies; DATA ANALYSIS The consensus DNA sequence was obtained, checked visually using the DNA Baser software ( and ambiguous ends were removed. All sequences were compared with the existing Barcode of Life Data (BOLD; and GenBank ( databases for sample identification using the BOLD Identification tool and basic local-alignment search tool (BLAST), respectively. DNA sequences, images and geographic location of all ichthyoplankton were deposited in BOLD under accession numbers LARVA00114 LARVA09714.
4 4 R. A. BECKER ET AL. (a) (b) (c) (d) (e) (f) Morphology Migratory Migratory? Non-migratory Non-migratory Non-migratory DNA barcode P. maculatus (M) P. maculatus (M) P. maculatus (M) L. octofasciatus (N-M) L. octofasciatus (N-M) P. maculatus (M) Fig. 1. Morphological and DNA barcode identification of neotropical fish eggs. (a, b), Eggs were identified as migratory fish species due to the reduced perivitellinic space. (d, e, f) Eggs presenting developed perivitellinic space were classified as non-migratory species. The visualization of perivitellinic space of egg (c) was hampered due to the presence of collated organic matter. Eggs identified through DNA barcoding as migratory species Pimelodus maculatus (a, b, c, f) varied widely in their perivitellinic space, similar to eggs of the non-migratory species Leporinus octofasciatus (d, e). Migratory and non-migratory species are indicated as M and N-M, respectively. RESULTS MORPHOLOGICAL IDENTIFICATION A total of 97 ichthyoplankton samples, consisting of 40 eggs and 57 larvae, from the upper Paraná River and the São Francisco River were analysed. Eggs varied in size (mean ± s.e. = 1 00 ± 0 13 mm), colour, shape and integrity state (Fig. 1). Morphological identification according to the perivitellinic space resulted in the classification of 16% as belonging to migratory species as they showed a large perivitellinic space, and 84% as non-migratory due to a reduced perivitellinic space (Table I). Larvae had mean ± s.d. L T of 4 75 ± 0 27 mm and varied greatly in their ontogenetic stage, colour and integrity state (Fig. 2). Classification of the larvae s ontogenetic stage consisted of 9% having embryonic larval yolk-sac development, 65% were at preflexion stage (disappearance of yolk and no notochord flexion), 14% were at flexion stage (showing initial notochord flexion and initial caudal fin rays) and 12% were at postflexion (complete notochord flexion and dorsal-fin rays). None of the larvae were identified to the species level, 3 5% were identified to the genus level (Hoplias sp.), 8 8% to the family and 75 4% to the order level. Detailed taxonomic identification from LGC-PUC laboratory revealed that 77 2% of all larvae Table I. Rates of morphological and DNA barcode identification of 40 fish eggs belonging to migratory or non-migratory species Morphology DNA barcode % n % n Migratory Non-migratory Total
5 DNA BARCODING OF NEOTROPICAL ICHTHYOPLANKTON 5 Ontogenetic stage Morphology DNA barcode (a) Larval yolk sac Characidae K. moenkausii (b) Preflexion Serrasalmus sp. S. brandtii (c) Flexion Apareiodon sp. A. affinis (d) Postflexion Apareiodon sp. A. affinis (e) Preflexion? A. affinis Fig. 2. Morphological and DNA barcode identification of neotropical fish larvae. Distinct ontogenetic larval stages: (a) larval yolk sac, (b) preflexion, (c) flexion, (d) postflexion and their identification based on morphology and DNA barcoding are shown. Larva (e) is an example of a damaged sample lacking any useful taxonomic characteristic. belonged to the order Characiformes, 1 7% to the order Cyprinodontiformes, 3 5% to the order Gymnotiformes and 5 3% to the order Siluriformes. The family Anostomidae was represented by 1 7%, Characidae by 5 3% and Poecilidae by 1 7% of all larval samples. Due to morphological damage, 12 3% of all larvae were not considered for identification. Larval identification conducted by NUPELIA laboratory allowed the identification of 24 5% to the genus level, 57 9% to family level and 5 3% to order level (Table II). Larvae identified to the genus level consisted of 3 5% Astyanax sp., 8 8% Apareiodon sp., 5 3% Serrasalmus sp.,3 5% Hoplias sp. and 3 5% Eigenmannia sp. Identification to family level found 1 7% Anostomidae, 1 7% Poecilidae and 54 4% Characidae, and 5 3% of larvae were identified to the order Siluriformes. Due to damaged larvae, 12 3% were not considered for identification. Higher taxonomic morphological identification of larvae was strongly correlated to ontogenetic development (Table II). For instance, when considering PUC-LGC s results, no larvae at yolk-sac stage were identified to the genus level, but identified 20% to the family and 80% to order level. At the preflexion stage, 81% of larvae could be identified to order level and 19% were too damaged to be classified. Considering the flexion ontogenetic stage, 12 5% of the larvae could be identified to the genus, 12 5% to the family and 75% to the order level. Taxonomical identification results obtained by NUPELIA identified 60% to order and 40% to family level of larvae at yolk-sac stage. Furthermore, 68% of larvae at preflexion stage were identified to family, 13% to genus level and 19% were not identified due to morphological damage. At flexion ontogenetic stage, 12% of larvae were identified to family and 88% to the
6 6 R. A. BECKER ET AL. Table II. Percentage accuracies of ichthyoplankton identification to each taxonomic level by morphology (Laboratory LGC-PUC and Laboratory NUPELIA) and DNA barcoding Taxonomic level Yolk sac Preflexion Flexion Postflexion LGC-PUC Species Genus Family Order 80 81* NUPELIA Species Genus Family 40 68* Order DNA barcode Species *Some larvae were damaged and therefore not included. LGC-PUC, Conservation Genetics Laboratory, Pontifícia Universidade Católica de Minas Gerais, Brazil; NUPELIA, Ichthyoplankton Laboratory, Núcleo de Pesquisas em Limnologia e Aquicultura, State University of Maringá, Brazil. genus level. At the postflexion stage, 57% of larvae were identified to family and 43% to the genus level (Table II). MOLECULAR IDENTIFICATION (DNA BARCODING) Barcode sequences (684 bp on average) for all 40 eggs and 57 larvae were successfully obtained. Barcodes obtained from eggs and larvae were compared against the GenBank and BOLD databases. For all barcodes, DNA sequence similarities were >99% (Tables SI and SII, Supporting Information). Unexpectedly, only nine eggs were similarly designated to migratory or nonmigratory species using morphological features when compared with DNA barcoding identification, resulting in an accuracy rate of 22 5%. DNA barcoding results showed that 67 5% of all eggs belonged to a single migratory species: Pimelodus maculatus Lacépède 1803 and 32 5% belonged to four non-migratory species: Galeocharax knerii (Steindachner 1879) (12 5%), Leporinus octofasciatus Steindachner 1915 (10%), Leporinus paranensis Garavello & Britski 1987 (2 5%) and Pimelodella meeki Eigenmann 1910 (7 5%) (Table SI, Supporting Information). When considering larval molecular identification, 14 species were recovered: Astyanax altiparanae Garutti & Britski 2000 (3 5%), Bryconamericus stramineus Eigenmann 1908 (1 8%), Knodus moenkhausii (Eigenmann & Kennedy 1903) (36 7%), Piabina argentea Reinhardt 1867 (7%), Apareiodon affinis (Steindachner 1879) (15 8%), Myleus micans (Lütken 1875) (10 5%), Serrasalmus brandtii Lütken 1875 (7%), Leporinus obtusidens (Valenciennes 1837) (1 8%), Hoplias malabaricus (Bloch 1794) (3 5%), Bergiaria westermanni (Lütken 1874) (3 5%), Iheringichthys labrosus (Lütken 1874) (1 8%), Pimelodus fur (Lütken 1874) (1 8%), Eigenmannia virescens (Valenciennes 1836) (3 5%) and Poecilia reticulata Peters 1859 (1 8%) (Table SII, Supporting Information). No discrepancy between DNA barcoding and morphologically identified larvae was observed. The morphologically identified larvae, however, were resolved to the family or genus level (Laboratory LGC-PUC 3 5% and Laboratory NUPELIA 24 6%); by using barcodes, all were identified to species with high sequence similarity (99 100%).
7 DNA BARCODING OF NEOTROPICAL ICHTHYOPLANKTON 7 DISCUSSION Identification through DNA barcoding overcame the lack of morphological characteristics and ontogenetic variation, allowing the identification of all sampled ichthyoplankton. DNA barcoding reached a greater taxonomic resolution complementing traditional morphological taxonomy. Due to difficulties in ichthyoplankton identification, DNA barcoding provides an important taxonomic tool for regions that already have barcode libraries available, such as the two river basins analysed in this study (Carvalho et al., 2011b; Pereira et al., 2013). The low accuracy rate (25%) observed using morphological techniques when identifying eggs as belonging to migratory or non-migratory species (Table I) clearly demonstrates that the large perivitellinic space for migratory species and reduced perivitellinic space for non-migratory species (Fig. 1) are not informative morphological characteristics. For instance, eggs from the non-migratory species L. octofasciatus, identified by DNA barcoding, showed a developed perivitellinic space [Fig. 1(d)] while the migratory species P. maculatus showed a reduced perivitellinic space [Fig. 1(a)]. Migratory and non-migratory eggs also showed similar shape, colour, size and perivitellinic space (Fig. 1). Moreover, great intraspecific morphological variations were observed for the eggs of P. maculatus [Fig. 1(a), (b), (c), (f)]. In some cases, the presence of collated organic matter [Fig. 1(c)] and damaged morphological characteristics made any morphological taxonomic inference impossible [Fig. 1(c)]. Shao et al. (2002) reported that morphological characteristics were less informative when compared with molecular identification even when more refined morphological techniques for identifying fish eggs were applied (i.e. light microscopy and scanning electron microscopy). Due to the low accuracy rates reported for morphological marine larvae identification when compared with DNA barcoding (Ko et al., 2013), a similar or lower accuracy when dealing with the megadiverse neotropical fish fauna was expected. Despite different levels of taxonomic resolution, disagreement when comparing morphological and DNA barcoding identification was not observed. Larval identification using DNA barcoding, however, resulted in a better taxonomic resolution when compared with traditional morphological taxonomy from both laboratories, especially for the early embryonic phase (i.e. larval yolk-sac stage), as well as allowing damaged sample identification (Table II). For instance, no larvae at larval yolk-sac stage could be identified to the genus level, but when considering the last developmental ontogenetic stage of postflexion, 43% of larvae were correctly identified to the genus level (Table II). Larvae in the yolk-sac ontogenetic stage show very few taxonomically useful morphological characteristics, making their identification to genus level speculative (Fig. 2). In this ontogenetic phase, characteristics are similar between different species, including species belonging to different families (Snyder, 1981; Bialetzki et al., 1998). Specimens that presented damaged morphological characteristics, such as sample larvae (Fig. 2), were successfully identified as A. affinis by DNA barcoding only, which illustrates a recurrent problem when identifying ichthyoplankton solely based on morphological traits. Interestingly, through the use of DNA barcoding, eggs from species not yet registered for this section of the upper Paraná River were detected, such as: K. moenkhausii, L. paranensis and P. meeki, all showed high DNA sequence similarity (99 100%) (Table SI, Supporting Information). Species assessments conducted in the upper Paraná Basin (Nova Ponte Reservoir) have not detected these fishes (unpubl. data). As these are native species already described in the lower parts of the Paraná Basin, they may
8 8 R. A. BECKER ET AL. occur in the upper region but have not yet been collected by traditional survey methods. Therefore, this is the first record of those species in this region, based solely on molecular data from ichthyoplankton. In conclusion, the ichthyoplankton identification to the species level through DNA barcoding is an efficient tool for the neotropical megadiverse realm, complementing and enhancing traditional morphological taxonomy. The upper Paraná and São Francisco River basins have a large barcode data set available, due to barcode libraries being already available for their ichthyofauna (Carvalho et al., 2011b; Pereira et al., 2013), which explains the great rate of identification of eggs and larvae in the present work to the species level (100% of identification with % sequences similarities). With the unfolding of the Brazilian Barcode of Life Project (BrBol; ichthyoplankton identification by DNA barcoding may be applicable to all river basins in Brazil providing unprecedented information on fish reproduction effects due to damming, detection of species-specific reproduction sites, recovery of hidden fish biodiversity as well as benefiting environmental and fish management conservation in the neotropics. Moreover, the developing of non-destructive DNA extraction from larvae (Alvarado Bremer et al., 2014) will allow the preservation of larvae vouchers and the performance of re-evaluation of diagnostic morphological or meristic characteristics, helping integrate morphological and molecular ichthyoplankton taxonomy. The accuracy of species identification via DNA barcoding, however, is ultimately dependent on the accuracy of morphology-based taxonomic assessment of voucher specimens whose barcodes are available in databases such as GenBank and BOLD. We are grateful to A. Bialetzki and her team for larvae identification performed at NUPELIA; B. Sanches, I. Penido, L. Carvalho, G. N. Salvador, M. L. Pugedo and V. Borges from Genética da Conservação Lab (PUC Minas) for their assistance and helping with sample collection. We thank BrBol/CNPq (564953/2010-5), FAPEMIG, CEMIG (Projeto Peixe Vivo) and CAPES PRO-EQUIPAMENTOS (783380/2013) for financial support. Supporting Information Supporting Information may be found in the online version of this paper: Table SI. Summary of morphological and DNA barcode identification of eggs. Accuracy rate between morphological and barcoding identification is summarized at the end of the table Table SII. Summary of morphological and DNA barcode identification of larvae obtained from the upper Paraná River basin (UP) and the São Francisco River basin (SF). Due to damaged samples, some larvae were not considered for morphological identification ( ). Accuracy rate between morphological and barcoding identification is summarized at the end of the table References Agostinho, A. A., Gomes, L. C., Suzuki, H. I. & Júlio, H. F. Jr. (2003). Migratory fishes of the upper Paraná River basin, Brazil. In Migratory Fishes of South America: Biology, Fisheries and Conservation Status (Carolsfeld, J., Harvey, B., Ross, C. & Baer, A., eds), pp Victoria, BC: World Fisheries Trust. Agostinho, A. A., Marques, E. E., Agostinho, C. S., Almeida, D. D., Oliveira, R. D. & Melo, J. D. (2007). Fish ladder of Lajeado Dam: migrations on one-way routes? Neotropical Ichthyology 5, doi: /S
9 DNA BARCODING OF NEOTROPICAL ICHTHYOPLANKTON 9 Ahlstrom, E. H. & Moser, H. G. (1976). Eggs and larvae of fishes and their role in systematic investigations and in fisheries. Revue des Travaux de l Institut des Peches Maritimes 40, Alvarado Bremer, J. R., Smith, B. L., Moulton, D. L., Lu, C. P., & Cornic, M. (2014). Shake and stew: a non-destructive PCR ready DNA isolation method from a single preserved fish larva. Journal of Fish Biology 84, doi: /jfb Alves, C. B. M., Vieira, F. & Pompeu, P. S. (2007). Programa Zoneamento Ecológico Econômico. In Caderno Temático: Biodiversidade (Vizentin, R. R., ed.), pp Brasília: MMA/SEDR/SBF. Arroyave, J. & Stiassny, M. L. (2014). DNA barcoding reveals novel insights into pterygophagy and prey selection in distichodontid fishes (Characiformes: Distichodontidae). Ecology and Evolution 4, doi: /ece Baumgartner, G., Nakatani, K., Gomes, L. C., Bialetzki, A. & Sanches, P. V. (2004). Identification of spawning sites and natural nurseries of fishes in the upper Paraná River, Brazil. Environmental Biology of Fishes 71, doi: /s z Bialetzki, A., Sanches, P. V., Baumgartner, G. & Nakatani, K. (1998). Caracterização morfológica e distribuição temporal de larvas e juvenis de Apareiodon affinis (Steindachner) (Osteichthyes, Parodontidae) no alto rio Paraná, Paraná. Revista Brasileira de Zoologia 15, doi: /S Bialetzki, A., Baumgartner, G., Sanches, P. V., Galuch, A. V., Luvisto, M. A., Nakatani, K., Makrakis, M. C. & Borges, M. E. E. (2001). Caracterização do desenvolvimento inicial de Auchenipterus osteomystax (Osteichthyes, Auchenipteridae) da bacia do rio Paraná, Brasil. Acta Scientiarum Biological Sciences 23, doi: /actascibiolsci.v23i Bialetzki, A., Sanches, P. V., Baumgartner, G., Makrakis, M. C. & Taguti, T. L. (2008). Desenvolvimento inicial de Hoplias aff. malabaricus (Bloch, 1794) (Osteichthyes, Erythrinidae) da planície alagável do alto rio Paraná, Brasil. Acta Scientiarum Biological Sciences 30, doi: /actascibiolsci.v30i Buckup, P. A., Menezes, A. & Ghazzi, N. A. (2007). Catálogo das espécies de peixes de água doce do Brasil, Vol. 1. Rio de Janeiro: Museu Nacional. Carreon-Martinez, L., Johnson, T. B., Ludsin, S. A. & Heath, D. D. (2011). Utilization of stomach content DNA to determine diet diversity in piscivorous fishes. Journal of Fish Biology 78, doi: /j x Carvalho, D. C., Neto, D. A., Brasil, B. S. & Oliveira, D. A. (2011a). DNA barcoding unveils a high rate of mislabeling in a commercial freshwater catfish from Brazil. Mitochondrial DNA 22, doi: / Carvalho, D. C., Oliveira, D. A., Pompeu, S., Leal, C. G., Oliveira, C. & Hanner, R. (2011b). Deep barcode divergence in Brazilian freshwater fishes: the case of the São Francisco River basin. Mitochondrial DNA 22, / DeSalle, R. & Amato, G. (2004). The expansion of conservation genetics. Nature Reviews Genetics 5, doi: /nrg1425 Gleason, L. U. & Burton, R. S. (2012). High throughput molecular identification of fish eggs using multiplex suspension bead arrays. Molecular Ecology Resources 12, doi: /j x Gogola, T. M., Daga, V. S., da Silva, P. R., Sanches, P. V., Gubiani, E. A., Baumgartner, G. & Delariva, R. L. (2010). Spatial and temporal distribution patterns of ichthyoplankton in a region affected by water regulation by dams. Neotropical Ichthyology 8, doi: /eff Graça, W. J. & Pavanelli, C. S. (2007). Peixes da planície de inundação do alto rio Paraná e áreas adjacentes. Maringá: Editora da Universidade Estadual de Maringá. Hermes-Silva, S., Reynalte-Tataje, D. & Zaniboni-Filho, E. (2009). Spatial and temporal distribution of ichthyoplankton in the upper Uruguay River, Brazil. Brazilian Archives of Biology and Technology 52, /S Ko, H. L., Wang, Y. T., Chiu, T. S., Lee, M. A., Leu, M. Y., Chang, K. Z. & Shao, K. T. (2013). Evaluating the accuracy of morphological identification of larval fishes by applying DNA barcoding. PLoS One 8, e doi: /journal.pone
10 10 R. A. BECKER ET AL. Langeani, F., Castro, R. M. C., Oyakawa, O. T., Shibatta, A. O., Pavanelli, C. S. & Casatti, L. (2007). Diversidade da ictiofauna do Alto Rio Paraná: composição atual e perspectivas futuras. Biota Neotropica 7, Leite, R. G., Cañas, C., Forsberg, B., Barthem, R. & Goulding, M. (2007). Larvas dos grandes bagres migradores. Manaus: Instituto Nacional de Pesquisas da Amazônia/Asociación para La Conservación de la Cuenca Amazónica. Loh, W. K. W., Bond, P., Ashton, K. J., Roberts, D. T. & Tibbetts, I. R. (2014). DNA barcoding of freshwater fishes and the development of a quantitative qpcr assay for the species-specific detection and quantification of fish larvae from plankton samples. Journal of Fish Biology 85, doi: /jfb Moura, T., Silva, M. C., Figueiredo, I., Neves, A., Muñoz, P. D., Coelho, M. M. & Gordo, L. S. (2008). Molecular barcoding of north-east Atlantic deep-water sharks: species identification and application to fisheries management and conservation. Marine and Freshwater Research 59, doi: /mf07192 Nakatani, K., Agostinho, A. A., Baumgartner, G., Bialetzki, A., Sanches, P. V., Makrakis, M. C. & Pavanelli, C. S. (2001). Ovos e larvas de peixes de água doce: desenvolvimento e manual de identificação, 1st edn. Maringá: Editora da Universidade Estadual de Maringá EDUEM. Oliveira, E. C. & Ferreira, E. J. G. (2008). Spawning areas, dispersion and microhabitats of fish larvae in the Anavilhanas Ecological Station, Rio Negro, Amazonas State, Brazil. Neotropical Ichthyology 6, doi: /S Oliveira, E. C., Bialetzki, A. & Assakawa, L. F. (2008). Morphological development of Hypophthalmus fimbriatus and H. marginatus post-yolk-sac larvae (Siluriformes: Pimelodidae). Zootaxa 1707, Pereira, L. H., Hanner, R., Foresti, F. & Oliveira, C. (2013). Can DNA barcoding accurately discriminate megadiverse Neotropical freshwater fish fauna? BMC Genetics 14, 20. doi: / Reis, R. E., Kullander, S. O. & Ferraris, C. J. (2003). CLOFFSCA-Check List of the Freshwater Fishes of South and Central America, 1st edn. Porto Alegre: Editora da Pontifícia Universidade do Rio Grande do Sul (EDIPUCRS). Reynalte-Tataje, D., Zaniboni-Filho, E. & Esquivel, J. R. (2004). Embryonic and larvae development of piracanjuba, Brycon orbignyanus Valenciennes, 1849 (Pisces, Characidae). Acta Scientiarum Biological Sciences 26, Richardson, D. E., Vanwye, J. D., Exum, A. M., Cowen, R. K. & Crawford, D. L. (2007). High throughput species identification: from DNA isolation to bioinformatics. Molecular Ecology Notes 7, doi: /j x Shao, K. T., Chen, K. C. & Wu, J. H. (2002). Identification of marine fish eggs in Taiwan using light microscopy, scanning electric microscopy and mtdna sequencing. Marine and Freshwater Research 53, doi: /MF01141 Snyder, D. E. (1981). Contributions to a Guide to the Cypriniform Fish Larvae of the Upper Colorado Rover System in Colorado. Denver, CO: Colorado State Office of the US Bureau of Land Management and Non-game Section of the Colorado Division of Wildlife. Sunnucks, P. & Hales, D. F. (1996). Numerous transposed sequences of mitochondrial cytochrome oxidase I-II in aphids of the genus Sitobion (Hemiptera: Aphididae). Molecular Biology and Evolution 13, Valdez-Moreno, M., Vásquez-Yeomans, L., Elías-Gutiérrez, M., Ivanova, N. V. & Hebert, P. D. (2010). Using DNA barcodes to connect adults and early life stages of marine fishes from the Yucatan Peninsula, Mexico: potential in fisheries management. Marine and Freshwater Research 61, doi: /MF09222 Ward, R. D., Hanner, R. & Hebert, P. D. (2009). The campaign to DNA barcode all fishes, FISH-BOL. Journal of Fish Biology 74, doi: /j x
MAPPING THE CRITICAL HABITATS FOR MIGRATORY SPECIES OF THE UPPER GRANDE RIVER REGION, MINAS GERAIS STATE, BRAZIL
- 645 - MAPPING THE CRITICAL HABITATS FOR MIGRATORY SPECIES OF THE UPPER GRANDE RIVER REGION, MINAS GERAIS STATE, BRAZIL SUZUKI, F.M. 1 ZAMBALDI, L.P.* POMPEU, P.S.* 1 Universidade Federal de Lavras University
More informationDistribution and abundance of fish eggs and larvae in three tributaries of the Upper Uruguay River (Brazil)
Environ Biol Fish (2011) 91:51 61 DOI 10.1007/s10641-010-9759-x Distribution and abundance of fish eggs and larvae in three tributaries of the Upper Uruguay River (Brazil) Rogério Nappi Corrêa & Samara
More informationPools and rapids as spawning and nursery areas for fish in a river stretch without floodplains
Neotropical Ichthyology, 2014 Copyright 2014 Sociedade Brasileira de Ictiologia DOI: 10.1590/1982-0224-20130116 Pools and rapids as spawning and nursery areas for fish in a river stretch without floodplains
More informationΙχθυοπλαγκτόν. Icthyoplankton. G. Koumoundouros. (L.A. Fuiman)
Ιχθυοπλαγκτόν Icthyoplankton (L.A. Fuiman) G. Koumoundouros Huge variety of reproductive patterns in finfish (internal or external fertilization, ovoviparity, oviparity, viviparity, with or without parental
More informationTemporal distribution of ichthyoplankton in the Forquilha river, upper Uruguay river Brazil: Relationship with environmental factors
Acta Scientiarum http://www.uem.br/acta ISSN printed: 1679-9283 ISSN on-line: 1807-863X Doi: 10.4025/actascibiolsci.v36i1.17993 Temporal distribution of ichthyoplankton in the Forquilha river, upper Uruguay
More informationNotes on Stygichthys typhlops (Characiformes; Characidae): characterization of their teeth and discussion about their diet
Notes on Stygichthys typhlops (Characiformes; Characidae): characterization of their teeth and discussion about their diet Francisco Alexandre C. Sampaio 1, Paulo Santos Pompeu 2 & Rodrigo Lopes Ferreira
More informationReceived February 11, 2004; accepted in principle May 7, 2004; accepted for publication June 25, 2004; published online August 3, 2004
Eggs and larvae of the curvina Plagioscion squamosissimus (Heckel, 1840) (Osteichthyes, Sciaenidae) in the Baía River, Mato Grosso do Sul State, Brazil ANDRÉA BIALETZKI 1,2 *, KESHIYU NAKATANI 1,2, PAULO
More informationSPAWNING AND NURSERY HABITATS OF NEOTROPICAL FISH SPECIES IN THE TRIBUTARIES
Chapter SPAWNING AND NURSERY HABITATS OF NEOTROPICAL FISH SPECIES IN THE TRIBUTARIES OF A REGULATED RIVER Maristela C. Makrakis 1, Patrícia S. da Silva 1, Sergio Makrakis 1, Ariane F. de Lima 1, Lucileine
More informationBarcoding the Fishes of North America. Philip A. Hastings Scripps Institution of Oceanography University of California San Diego
Barcoding the Fishes of North America Philip A. Hastings Scripps Institution of Oceanography University of California San Diego With the possible exception of Europe and selected regional faunas such as
More informationSpatial and temporal variation of the ichthyoplankton in a subtropical river in Brazil
Environ Biol Fish (2012) 94:403 419 DOI.07/s641-011-9955-3 Spatial and temporal variation of the ichthyoplankton in a subtropical river in Brazil David Augusto Reynalte-Tataje & Angelo Antônio Agostinho
More informationGenetic analysis of radio-tagged westslope cutthroat trout from St. Mary s River and Elk River. April 9, 2002
Genetic analysis of radio-tagged westslope cutthroat trout from St. Mary s River and Elk River April 9, 2002 Report prepared for: Angela Prince, M.Sc., R.P. Bio Westslope Fisheries 517 13 th Avenue South
More informationDeep barcode divergence in Brazilian freshwater fishes: the case of the São Francisco River basin
Mitochondrial DNA, October 2011; 22(S1): 80 86 Deep barcode divergence in Brazilian freshwater fishes: the case of the São Francisco River basin DANIEL C. DE CARVALHO 1,2, DENISE A. A. OLIVEIRA 2, PAULO
More informationSchaft Creek Project: Fisheries Baseline 2008 Addendum
Copper Fox Metals Inc. Schaft Creek Project: Fisheries Baseline 2008 Addendum Rescan Tahltan Environmental Consultants Sixth Floor - 1111 West Hastings Street Vancouver, BC Canada V6E 2J3 Tel: (604) 689-9460
More informationPassage of fish larvae and eggs through the Funil, Itutinga and Camargos Reservoirs on the upper Rio Grande (Minas Gerais, Brazil)
Neotropical Ichthyology, 9(3): 617-622, 2011 Copyright 2011 Sociedade Brasileira de Ictiologia Passage of fish larvae and eggs through the Funil, Itutinga and Camargos Reservoirs on the upper Rio Grande
More informationCharacterization of two microsatellite PCR multiplexes for high throughput. genotyping of the Caribbean spiny lobster, Panulirus argus
Characterization of two microsatellite PCR multiplexes for high throughput genotyping of the Caribbean spiny lobster, Panulirus argus Nathan K. Truelove 1, Richard F. Preziosi 1, Donald Behringer Jr 2,
More informationChec List Journal of species lists and distribution
Check List 8(6): 1158 1162, 2012 2012 Check List and Authors ISSN 1809-127X (available at www.checklist.org.br) Chec List Journal of species lists and distribution L i s t s of Species Fishes from first
More informationTemporal variability of fish larvae assemblages: influence of natural and anthropogenic disturbances
Neotropical Ichthyology, 10(4):837-846, 2012 Copyright 2012 Sociedade Brasileira de Ictiologia Temporal variability of fish larvae assemblages: influence of natural and anthropogenic disturbances David
More informationSpawning areas, dispersion and microhabitats of fish larvae in the Anavilhanas Ecological Station, rio Negro, Amazonas State, Brazil
Neotropical Ichthyology, 6(4):559-566, 2008 Copyright 2008 Sociedade Brasileira de Ictiologia Spawning areas, dispersion and microhabitats of fish larvae in the Anavilhanas Ecological Station, rio Negro,
More informationIdentification of fish nursery areas in a free tributary of an impoundment region, upper Uruguay River, Brazil
Neotropical Ichthyology, 10(2): 425-438, 2012 Copyright 2012 Sociedade Brasileira de Ictiologia Identification of fish nursery areas in a free tributary of an impoundment region, upper Uruguay River, Brazil
More informationUtilization of the fish ladder at the Engenheiro Sergio Motta Dam, Brazil, by long distance migrating potamodromous species
Neotropical Ichthyology, 5(2):197-204, 2007 Copyright 2007 Sociedade Brasileira de Ictiologia Utilization of the fish ladder at the Engenheiro Sergio Motta Dam, Brazil, by long distance migrating potamodromous
More informationObserved pattern of diel vertical migration of Pacific mackerel larvae and its implication for spatial distribution off the Korean Peninsula
SPF-S2_Lee Observed pattern of diel vertical migration of Pacific mackerel larvae and its implication for spatial distribution off the Korean Peninsula Hwahyun Lee 1, Sukyung Kang 2, Kyungmi Jung 2, Jung-Jin
More informationSummary of Research within Lamlash Bay No-Take Zone - Science report for COAST July
Summary of Research within Lamlash Bay No-Take Zone - Science report for COAST July 2013 - *Picture of a spider crab (Macropodia spp) inside a plumose anemone. Taken within Lamlash Bay No-Take Zone by
More informationEffects of reservoirs water level variations on fish recruitment
Journal homepage: www.scielo.br/ni DOI: 10.1590/1982-0224-20160084 Published online: 05 October 2017 (ISSN 1982-0224) Copyright 2017 Sociedade Brasileira de Ictiologia Printed: 29 September 2017 (ISSN
More informationLINKING THE TAIWAN FISH DATABASE TO THE GLOBAL DATABASE
LINKING THE TAIWAN FISH DATABASE TO THE GLOBAL DATABASE Kwang-Tsao Shao*, Jack Yung-Chang Lin, and Hsin-Hua Lin Research Center for Biodiversity, Academia Sinica, Taiwan, R.O.C. No. 128, Academia Road
More informationDevelopment and Identification of Three Species of Thai Ricefish, Oryzias, in the Mekong Basin
Tropical Natural History 12(1): 75-88, April 2012 2012 by Chulalongkorn University Development and Identification of Three Species of Thai Ricefish, Oryzias, in the Mekong Basin APICHART TERMVIDCHAKORN
More informationPeixes de água doce da Mata Atlântica. Naércio A. Menezes Museu de Zoologia da USP
Peixes de água doce da Mata Atlântica Naércio A. Menezes Museu de Zoologia da USP Mapa da Mata Atlântica sensu strictu com domínios 25 e 26 definidos por Hueck & Seibert (1981) 309 species listed 267 endemic
More informationCutthroat trout genetics: Exploring the heritage of Colorado s state fish
Cutthroat trout genetics: Exploring the heritage of Colorado s state fish Metcalf et al. 2007 Molecular Ecology Metcalf et al. 2007 From Metcalf et al. 2007 But what about this one? Metcalf et al. 2007
More informationApplication of DNA Barcoding Techniques For Speciation
Application of DNA Barcoding Techniques For Speciation Bruno J. Giri US Department of Agriculture Office of Public Health Science 1 Objectives Background of catfish speciation Background of DNA barcoding
More informationIchthyofauna of the Una river in the Paraíba do Sul Paulista River Valley, Southeastern of Brazil
Biota Neotropica 18(4): e20180528, 2018 www.scielo.br/bn ISSN 1676-0611 (online edition) Inventory Ichthyofauna of the Una river in the Paraíba do Sul Paulista River Valley, Southeastern of Brazil Juliano
More informationBIOLOGY OF FISHES FISH/BIOL 311
BIOLOGY OF FISHES FISH/BIOL 311 FORM, FUNCTION, AND BIODIVERSITY: EARLY-LIFE HISTORY, EGGS AND LARVAE, TECHNIQUES AND APPROACHES, ONTOGENY AND PHYLOGENY General topics: 1. Introduction 2. Sampling of eggs
More informationMaintaining biodiversity in mixed-stock salmon fisheries in the Skeena watershed
Maintaining biodiversity in mixed-stock salmon fisheries in the Skeena watershed A 130-year history Chris C. Wood Principle: Mixed-stock transboundary fisheries impose special requirements on management
More informationWeedy Seadragon Ecology Project. Annual Report Underwater Research Group of NSW Fish Ecology Lab, UTS
Weedy Seadragon Ecology Project Annual Report 2015 Underwater Research Group of NSW Fish Ecology Lab, UTS 1. Summary UTS and URG forged an informal partnership in 2015, to further the seadragon research
More informationNATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi
NATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi For office use only MARINE BIORESOURCES FORMS DATA ENTRY: Form- 1(general ) Ref. No.: (please answer only relevant
More informationMetadata Freshwater Fishes of Colombia
Metadata Freshwater Fishes of Colombia Exported from the Freshwater Biodiversity Data Portal, http://data.freshwaterbiodiversity.eu Visit the Freshwater Metadatabase, http://data.freshwaterbiodiversity.eu/metadb/about_metadata
More informationArticle. Abstract. Introduction
Zootaxa 2008: 1 22 (2009) www.mapress.com/zootaxa/ Copyright 2009 Magnolia Press Article ISSN 1175-5326 (print edition) ZOOTAXA ISSN 1175-5334 (online edition) Genetic identification and color descriptions
More information8 TH MEETING DOCUMENT BYC-08 INF-A
INTER-AMERICAN TROPICAL TUNA COMMISSION WORKING GROUP ON BYCATCH 8 TH MEETING La Jolla, California (USA) 10-11 May 2018 DOCUMENT BYC-08 INF-A WHALE SHARK INTERACTIONS WITH THE TUNA PURSE-SEINE FISHERY
More informationActa Scientiarum. Biological Sciences ISSN: Universidade Estadual de Maringá Brasil
Acta Scientiarum. Biological Sciences ISSN: 1679-9283 eduem@uem.br Universidade Estadual de Maringá Brasil Kipper, Darlon; Leika Taguti, Tátia; Bialetzki, Andréa; Cavicchioli Makrakis, Maristela; Baumgartner,
More informationFIRST RECORD OF HYPOSTOMUS PECKOLTOIDES (SILURIFORMES: LORICARIIDAE) IN PARAGUAY WITH COMMENTS ON VARIATIONS
Bol. Mus. Nac. Hist. Nat. Parag. Vol. 17, nº 1 (Ago. 2013): 100-100 67-71 FIRST RECORD OF HYPOSTOMUS PECKOLTOIDES (SILURIFORMES: LORICARIIDAE) IN PARAGUAY WITH COMMENTS ON VARIATIONS Diego Bueno Villafañe
More informationScientific Note Distribution extension of Cyphocharax spilotus
Scientific Note Distribution extension of Cyphocharax spilotus (Vari, 1987) (Characiformes: Curimatidae), at Patos-Mirim lagoon system, Rio Grande do Sul State, Brazil FABIANO CORRÊA 1*, THIAGO TUCHTENHAGEN
More informationNATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi
NATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi MARINE BIORESOURCES FORMS DATA ENTRY: Form- 1(general) For office use: Fauna: Flora Microorganisms General Category:
More informationVersion: 1.0 Last amendment: 16/01/2012. Contact Officer: Animal Welfare Officer
CDU AEC Policy on the Collection of Voucher Specimens : 1.0 Last amendment: 16/01/2012 Contact Officer: Animal Welfare Officer Approved By: CDU AEC Date: January 2012 Next Review: January 2014 INTRODUCTION
More informationMolecular identification of Cichla (Perciformes: Cichlidae) introduced in reservoirs in Southern Brazil
Acta Scientiarum http://www.uem.br/acta ISSN printed: 1679-9283 ISSN on-line: 1807-863X Doi: 10.4025/actascibiolsci.v35i2.10684 Molecular identification of Cichla (Perciformes: Cichlidae) introduced in
More information1 Introduction. DNA Barcodes 2015; 3: DOI /dna Received February 21, 2015; accepted July 6, 2015
DNA Barcodes 2015; 3: 129 138 Research Article Open Access Camila da Silva de Souza, Claudio Oliveira, Luiz Henrique Garcia Pereira* Knodus moenkhausii (Characiformes: Characidae): one fish species, three
More informationContinued Genetic Monitoring of the Kootenai Tribe of Idaho White Sturgeon Conservation Aquaculture Program
Continued Genetic Monitoring of the Kootenai Tribe of Idaho White Sturgeon Conservation Aquaculture Program Deliverable 1): Monitoring of Kootenai River white sturgeon genetic diversity Deliverable 2):
More information!"#$%&'() Mola mola *+,+-./
Mola mola 2008 Summary A study on the reproductive biology of ocean sunfish Mola mola Toshiyuki akatsubo 1. Introduction Ocean sunfish, Mola mola, which belongs to the family Molidae in the order Tetraodontiformes,
More informationStocking success of Scottish Atlantic salmon in two Spanish rivers
Journal of Fish Biology (1997) 51, 1265 1269 Stocking success of Scottish Atlantic salmon in two Spanish rivers E. VERSPOOR* AND C. GARCIA DE LEÁNIZ *Marine Laboratory, P.O. Box 101, Aberdeen AB11 9DB,
More informationExtract from the project Dynamic Mapping of North Sea Spawning - the KINO Report 2016 Statoil contract no
Extract from the project Dynamic Mapping of North Sea Spawning - the KINO Report 2016 Statoil contract no. 4503121426 5.3.1 Sandeels Ammodytidae - tobis There are five species of sandeel in the North Sea
More informationMorphological and Molecular Identification of species of Catfish Genus Cranoglanis from Lam River, Nghe an, Vietnam
ISSN No. (Print): 075-10 ISSN No. (Online): 4- Morphological and Molecular Identification of species of Catfish Genus Cranoglanis from am River, Nghe an, Vietnam Nguyen Dinh Vinh *, Tran Thi Thuy Ha **,
More informationWNY PRISM Partners Meeting: Using edna to Detect and Monitor Invasive Species in New York State
WNY PRISM Partners Meeting: Using edna to Detect and Monitor Invasive Species in New York State Rod Getchell, Fina Casey, Jim Casey, Dave MacNeill, and Donna Cassidy-Hanley Aquatic Animal Health Program
More informationSteelhead Kelt Reconditioning and Reproductive Success Studies in the Columbia River Basin
Steelhead Kelt Reconditioning and Reproductive Success Studies in the Columbia River Basin Hatch, 1 D.R., D.E. Fast 2, W.J. Bosch 2, J.W. Blodgett 2, J.L.J. Trammell 2, A.L. Pierce, 1,3 S.R. Everett 4,
More informationDiet of a Catfish before and after Damming of the Salto Caxias Reservoir, Iguaçu River
767 Vol.5, n. 5 : pp. 767-775 September 7 ISSN 1516-8913 Printed in Brazil BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY AN INTERNATIONAL JOURNAL Diet of a Catfish before and after Damming of the Salto
More informationCytogenetic Analysis of Three Sympatric Gymnotus Species (Teleostei: Gymnotidae) from the Fundo Stream, MG, Brazil
2007 The Japan Mendel Society Cytologia 72(1): 89 93, 2007 Cytogenetic Analysis of Three Sympatric Gymnotus Species (Teleostei: Gymnotidae) from the Fundo Stream, MG, Brazil Maria Conceição Vieira Lacerda
More informationArtur Antonio Andreata 1, Claudio Oliveira 2 and Fausto Foresti 2. Morfologia, Botucatu, SP, Brazil. Abstract. Introduction. Materials and Methods
Research Article Genetics and Molecular Biology, 29, 1, 62-66 (2006) Copyright by the Brazilian Society of Genetics. Printed in Brazil www.sbg.org.br Karyological characterization of four Neotropical fish
More informationTHE DIVERSITY OF FISHES
Gene S. Helfman Bruce B. Collette Douglas E. Facey Brian W. Bowen Second Edition THE DIVERSITY OF FISHES Biology, Evolution, and WILEY-BLACKWELL A John Wiley & Sons, Ltd., Publication Brief contents Full
More informationHatcheries: Role in Restoration and Enhancement of Salmon Populations
Hatcheries: Role in Restoration and Enhancement of Salmon Populations Hatcheries play a large role in the management, ecology, and evolution of Pacific salmon. Why were/are they built? What are the assumptions
More informationThe Emerging View of New England Cod Stock Structure
Cod Population Structure and New England Fisheries Symposium: Furthering our understanding by integrating knowledge gained through science and fishing Putting it All Together: The Emerging View of New
More informationA COMPARISON OF MICROPLASTIC INGESTION BETWEEN FRESHWATER SUNFISH AND MARINE PINFISH
A COMPARISON OF MICROPLASTIC INGESTION BETWEEN FRESHWATER SUNFISH AND MARINE PINFISH COLLEEN A. PETERS, PEYTON A. THOMAS, KAITLYN B. RIEPER AND SUSAN P. BRATTON*; Fiber BACKGROUND Sphere/microbead Fragment
More informationSWG JACK MACKEREL FISHERY IN CHILE
SWG-10-07 JACK MACKEREL FISHERY IN CHILE ANNUAL NATIONAL REPORT SCIENCE WORKING GROUP 2011 1. DESCRIPTION OF THE FISHERY. 1.1 Composition of the Fleet. The structure and size of the industrial purse seine
More informationUSING BIOLOGICALLY IMPORTANT PHYSICAL CHARACTERISTICS OF ESTUARIES TO CLASSIFY AUSTRALIAN AND NEW ZEALAND ESTUARIES
USING BIOLOGICALLY IMPORTANT PHYSICAL CHARACTERISTICS OF ESTUARIES TO CLASSIFY AUSTRALIAN AND NEW ZEALAND ESTUARIES Michael Whelan, Southern Cross University, Australia. Peter Saenger, Southern Cross University,
More informationAdvanced Animal Science TEKS/LINKS Student Objectives One Credit
First Six Weeks Career/Safety/Work Habits AAS 1(A) The student will identify career development and entrepreneurship opportunities in the field of animal systems. AAS 1(B) The student will apply competencies
More informationA new killifish of the genus Melanorivulus from the upper Paraná river basin, Brazil (Cyprinodontiformes: Rivulidae)
Senckenberg Gesellschaft für Naturforschung, 2013. 63 (3): 277 281 20.12.2013 A new killifish of the genus Melanorivulus from the upper Paraná river basin, Brazil (Cyprinodontiformes: Rivulidae) Wilson
More informationSalmon bycatch patterns in the Bering Sea pollock fishery
Salmon bycatch patterns in the Bering Sea pollock fishery James Ianelli Seattle, WA Data from the North Pacific Observer Program (Fisheries Monitoring and Assessment) were analyzed for seasonal, temporal,
More informationSpecies Identification of small juvenile tunas caught in surface fisheries in the Phili... 1/13 ページ
Species Identification of small juvenile tunas caught in surface fisheries in the Phili... 1/13 ページ Originated by: Fisheries and Aquaculture Department Title: Status of Interactions of Pacific Tuna Fisheries
More informationLegendre et al Appendices and Supplements, p. 1
Legendre et al. 2010 Appendices and Supplements, p. 1 Appendices and Supplement to: Legendre, P., M. De Cáceres, and D. Borcard. 2010. Community surveys through space and time: testing the space-time interaction
More informationSystematics and Biodiversity of the Order Cypriniformes (Actinopterygii, Ostariophysi) A Tree of Life Initiative. NSF AToL Workshop 19 November 2004
Systematics and Biodiversity of the Order Cypriniformes (Actinopterygii, Ostariophysi) A Tree of Life Initiative NSF AToL Workshop 19 November 2004 Gloria Arratia Nevin Aspinwall Hank Bart Miles Coburn
More informationReproductive traits of the yellow-mandi catfish Pime/odus maculatus Lacepede (Osteichthyes, Siluriformes) in captive breeding
Reproductive traits of the yellow-mandi catfish Pime/odus maculatus Lacepede (Osteichthyes, Siluriformes) in captive breeding Yoshimi Sato 1 Nelsy Fenerich-Verani 2 Jose Roberto Verani 2 Hugo Pereira Godinho
More informationChromosome Characterization of a Neotropical Fish Poptella paraguayensis from Paraguay River Basin
1998 The Japan Mendel Society Cytologia 63: 73-77,1998 Chromosome Characterization of a Neotropical Fish Poptella paraguayensis from Paraguay River Basin (Stethaprioninae, Characidae) P. D. Freitas1, M.
More informationNATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi
NATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi MARINE BIORESOURCES FORMS DATA ENTRY: Form- 1(general ) (please answer only relevant fields;add additional fields
More informationCarnivory during Ontogeny of the Plagioscion squamosissimus: A Successful Non-Native Fish in a Lentic Environment of the Upper Paraná River Basin
RESEARCH ARTICLE Carnivory during Ontogeny of the Plagioscion squamosissimus: A Successful Non-Native Fish in a Lentic Environment of the Upper Paraná River Basin Mayara Pereira Neves 1 *, Rosilene Luciana
More informationChagrin River TMDL Appendices. Appendix F
Appendix F The following are excerpts from the Eastern Brook Trout Joint Venture s Conservation Strategy (Working Draft v.6), Conserving the Eastern Brook Trout: Strategies for Action Found at: http://www.easternbrooktrout.org/constrategy.html
More informationDiversity of Thermophilic Bacteria Isolated from Hot Springs
... 40(2) 524-533 (2555) KKU Sci. J. 40(2) 524-533 (2012) Diversity of Thermophilic Bacteria Isolated from Hot Springs ( 30, 3.65 x 10 5 1. x 10 3 CFU/ml (a, b, c, d, e f 16S rrna 1. kb Polymerase Chain
More informationTeleosts: Evolutionary Development, Diversity And Behavioral Ecology (Fish, Fishing And Fisheries) READ ONLINE
Teleosts: Evolutionary Development, Diversity And Behavioral Ecology (Fish, Fishing And Fisheries) READ ONLINE If searched for a ebook Teleosts: Evolutionary Development, Diversity and Behavioral Ecology
More informationTemporal and Spatial Distribution of the Ichthyofauna in Two Streams of the Upper Rio Paraná Basin
271 Vol.46, n. 2 : pp. 271-280, March 2003 ISSN 1516-8913 Printed in Brazil BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY AN INTERNATIONAL JOURNAL Temporal and Spatial Distribution of the Ichthyofauna in
More informationNOTES ON EGGS, LARV^ AND JUVENILES OF FISHES FROM INDIAN WATERS
NOTES ON EGGS, LARV^ AND JUVENILES OF FISHES FROM INDIAN WATERS V. Euthynnus affinis (Cantor)* BY S. JONES {Central Marine Fisheries Research Station) LARVAL stages of Katsuwonus pelamis (Linnaeus) and
More informationMapping & modelling of winter ichthyoplankton distribution in the Channel & Southern North Sea
French Research Institute for Exploitation of the Sea Mapping & modelling of winter ichthyoplankton distribution in the Channel & Southern North Sea Method of work & Preliminary results Elvire Antajan,
More informationMigration, Behaviour and Habitat Selection by Anadromous Brook Trout, Salvelinus fontinalis (Mitchell), in a Nova Scotia Southern Upland:
Migration, Behaviour and Habitat Selection by Anadromous Brook Trout, Salvelinus fontinalis (Mitchell), in a Nova Scotia Southern Upland: FFRC Year-End REPORT E.A. Halfyard Dalhousie University and the
More informationAbstract. Resumo.
http://dx.doi.org/10.1590/1519-6984.179070 Effects of light-dark cycle on the spatial distribution and feeding activity of fish larvae of two co-occurring species (Pisces: Hypophthalmidae and Sciaenidae)
More informationZooplankton Availability to. Larval Walleye (Sander vitreus) in Black Lake, MI, USA
Zooplankton Availability to Larval Walleye (Sander vitreus) in Black Lake, MI, USA Dana Jo DePlonty School of Biological Science Dr. Kristi Arend 1 Abstract Black Lake has very few small walleye even though
More informationSusitna-Watana Hydroelectric Project Document ARLIS Uniform Cover Page
Alaska Resources Library & Information Services Document ARLIS Uniform Cover Page Title: Wildlife harvest analysis study, Study plan Section 10.20 : Final study plan SuWa 200 Author(s) Personal: Author(s)
More informationCytogenetic description of Ancistrus abilhoai (Siluriformes: Loricariidae) from Iguaçu River basin, southern Brazil
Cytogenetic description of Ancistrus abilhoai (Siluriformes: Loricariidae) from Iguaçu River basin, southern Brazil M.O. Ribeiro 1, R.B. Noleto 1, C.A. Lorscheider 1, F.E. Porto 2, A.C. Prizon 2, C.H.
More informationWhat do electronic tags offer in characterizing pelagic fish movement for stock assessment?
What do electronic tags offer in characterizing pelagic fish movement for stock assessment? Chi Hin Lam (Tim) University of Massachusetts Boston tagtuna@gmail.com Photo: Paul Murray Molly Lutcavage Benjamin
More informationActa Scientiarum. Biological Sciences ISSN: Universidade Estadual de Maringá Brasil
Acta Scientiarum. Biological Sciences ISSN: 1679-9283 eduem@uem.br Universidade Estadual de Maringá Brasil Oliveira Zeni, Jaquelini; Santos, Ana Cláudia; Rogério Carvalho, Fernando Contribution of different
More informationELECTRO-FISHING REPORT 2016 UPPER TWEED
ELECTRO-FISHING REPORT 2016 UPPER TWEED The electro-fishing programme carried out each summer by The Tweed Foundation is part of our management plan, which details the information that is required to manage
More informationRiffle and pool fish communities in a large stream of southeastern Brazil
Neotropical Ichthyology, 3(2):305-311, 2005 Copyright 2005 Sociedade Brasileira de Ictiologia Francisco Langeani*, Lilian Casatti*, Helena Seixas Gameiro*, André Bellucco do Carmo**, and Denise de Cerqueira
More informationThe Sustainability of Atlantic Salmon (Salmo salar L.) in South West England
The Sustainability of Atlantic Salmon (Salmo salar L.) in South West England Submitted by Sarah-Louise Counter to the University of Exeter as a thesis for the degree of Doctor of Philosophy in Biological
More informationNATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi
NATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi For office use: MARINE BIORESOURCES FORMS DATA ENTRY: Form- 1(general ) (please answer only relevant fields;add
More informationQuagga Mussels in the West and the Colorado River Basin. Ricardo De Leon, Ph.D. Metropolitan Water District of Southern California
Quagga Mussels in the West and the Colorado River Basin Ricardo De Leon, Ph.D. Metropolitan Water District of Southern California Invasive Mussels Live quagga mussels discovered January 6, 2007 in Lake
More informationQuick method for identifying horse (Equus caballus) and donkey (Equus asinus) hybrids
Short Communication Quick method for identifying horse (Equus caballus) and donkey (Equus asinus) hybrids M.M. Franco 1,2,3, J.B.F. Santos 2, A.S. Mendonça 3, T.C.F. Silva 2, R.C. Antunes 2 and E.O. Melo
More informationWhat DNA tells us about Walleye (& other fish) in the Great Lakes
What DNA tells us about Walleye (& other fish) in the Great Lakes Carol Stepien, Douglas Murphy, Rachel Lohner, & Jo Ann Banda Great Lakes Genetics Lab Lake Erie Center University of Toledo What do we
More informationSupporting genetic analysis of protected fish species. R. Armstrong
Supporting genetic analysis of protected fish species R. Armstrong Malcolm Francis Peter Ritchie Nine protected New Zealand fish species Giant manta ray White shark (R. Armstrong) Whale shark Basking shark
More informationAtsuko YAMAGUCHI. Since the catches of these fish decrease as the waters, including those around western Kyushu and
Atsuko YAMAGUCHI distributions, feeding habits, reproductive behavior, off the coast of Nagasaki, western Kyushu. It is growth, migration, population structure, and other relatively large biological aspects
More informationNATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi
NATIONAL BIORESOURCE DEVELOPMENT BOARD Dept. of Biotechnology Government of India, New Delhi MARINE BIORESOURCES FORMS DATA ENTRY: Form- 1(general) For office use: Fauna: Flora Microorganisms General Category:
More informationsubmitted: fall 2009
Cat Project of the Month April 2010 The IUCN/SSC Cat Specialist Group's website (www.catsg.org) presents each month a different cat conservation project. Members of the Cat Specialist Group are encouraged
More informationBLACK SEA WHITING, MERLANGIUS MERLANGUS EUXINUS NORDMANN
82 BLACK SEA WHITING, MERLANGIUS MERLANGUS EUXINUS NORDMANN The taxonomic position of the Black Sea whiting like that of a great number of Black Sea fish species is rather complicated, and is still a controversial
More informationINFLUENCE OF TRAFFIC FLOW SEPARATION DEVICES ON ROAD SAFETY IN BRAZIL S MULTILANE HIGHWAYS
INFLUENCE OF TRAFFIC FLOW SEPARATION DEVICES ON ROAD SAFETY IN BRAZIL S MULTILANE HIGHWAYS Gabriel Andrade Lanzaro Universidade de Brasília Departamento de Engenharia Civil e Ambiental, Faculdade de Tecnologia,
More informationAbundance and length structure of Brycon nattereri (Osteichthyes, Bryconidae), an endangered fish species in central Brazil
Acta Scientiarum http://www.uem.br/acta ISSN printed: 1679-9283 ISSN on-line: 1807-863X Doi: 10.4025/actascibiolsci.v36i4.23857 Abundance and length structure of Brycon nattereri (Osteichthyes, Bryconidae),
More informationCambridge International Examinations Cambridge International Advanced Subsidiary and Advanced Level
Cambridge International Examinations Cambridge International Advanced Subsidiary and Advanced Level *8315138911* MARINE SCIENCE 9693/04 Paper 4 A2 Data-Handling and Free-Response May/June 2015 1 hour 15
More informationAge and growth of the young swordfish Xiphias gladius L. in Taiwan waters using otolith. Chi-Lu Sun, Hsiao-Ling Lin, an Su-Zan Yeh
SCTB15 Working Paper BBRG-8 Age and growth of the young swordfish Xiphias gladius L. in Taiwan waters using otolith Chi-Lu Sun, Hsiao-Ling Lin, an Su-Zan Yeh Institute of Oceanography National Taiwan University
More informationEmployer Name: NOAA Fisheries, Northwest Fisheries Science Center
Internship Description Employer Name: NOAA Fisheries, Northwest Fisheries Science Center Employer Description: Manchester Research Station functions as a satellite facility to the NOAA Fisheries Northwest
More informationFirst occurrence of Serranus hepatus in the Bulgarian Black Sea coast
J. Black Sea/Mediterranean Environment Vol. 20, No. 2: 142-146 (2014) SHORT COMMUNICATION First occurrence of Serranus hepatus in the Bulgarian Black Sea coast Apostolou Apostolos * Institute of Biodiversity
More information