Results of a questionnaire on rotifer culture methods and protocols currently used in European hatcheries
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1 Results of a questionnaire on rotifer culture methods and protocols currently used in European hatcheries EU funded project: Genetic implications in the production of rotifers in commercial finfish hatcheries (Q5RS ) Rotifer workshop: 9 September 2005 Ghent University
2 Project partners: Laboratory for Aquaculture & Artemia Reference Center Ghent University, Belgium Sea Fisheries Department, Ministry of Agriculture, Ostend, Belgium Department of Genetics, Development and Molecular Biology Aristotle University of Thessaloniki, Greece Norwegian University of Science and Technology, Trondheim, Norway Maricoltura di Rosignano Solvay srl, Livorno, Italy
3 Location of the participating hatcheries 4 13 hatcheries 17 rotifer stock cultures
4 Which rotifer type are you using? S M L Not known B. plicatilis hatchery:? 10 hatcheries: 1 rotifer stock culture 2 hatcheries: more than 1 stock culture (2 or 4 stock cultures)
5 Origin of the rotifers cultured in the hatchery? Unknown: 7 Known: 10 Institute: 3 Other hatchery: 3 Place: USA: 1 France: 2 U.K.: 1 Transport of rotifer starters
6 Maintenance of stock cultures during the fish larvae production All hatcheries except 1 Maintenance of stock cultures outside the fish larvae production No: 3 Yes: 10 Source at the start of the new season: - other commercial farm - buy from a company - own resting eggs 1 hatchery: mass production year round
7 Feed: Stock cultures All hatcheries feed algae or a mix of algae. species: Tetraselmis (1), Nannochloropsis (3), Chlorella (2), Isochrysis (1) 1 hatchery: mix of algae, yeast, vitamines, oil Oxygen: Aeration: YES : filtered air (4) NO : (8) Rotating (4) Shaking (4)
8 Mass cultures Feed: 8 hatcheries apply mixtures of feeds 5 hatcheries apply artificial diets Mixtures: - Nanno/Chlor. paste (Reed Mariculture), fresh baker yeast, emulsified oil (Rich), Vit. - Nanno paste (Reed Mariculture), ROCULT (Dana Feed), Culture Selco 3000 (INVE), fresh baker yeast, emulsified oil (Marol E) - Nanno/ Chlor. paste, dry baker yeast (rarely) - Chlor. paste, fresh algae - Fresh algae, dry yeast (2) - Fresh algae, DHA prot Selco, fresh baker yeast - Dry feed (Algamac 2000), fresh baker yeast Artificial diets: - Culture Selco 3000 (INVE) (3) - Culture Selco Culture Selco Plus (INVE) - ROCULT (Dana Feed)
9 Mass cultures Feeding method: Manually: 8 hatcheries Continuously: 6 hatcheries Mixture: 3 Artificial diet: 5 Mixture: 5 Artificial diet: 1 Culture type: - Batch culture: 7 3 days culture period (5) 3-4 days (1) 10 days (1) - Semi-continuous: 3 - Continuous: 2 - Recirculation: 2
10 Suspected reasons for crashes Human error: 11 Ciliates: 7 Bacteria: 5 Oxygen: 5 Floccules: 2 Ammonia: 2 Change of feed-feeding regime: 2 Season related: 2 Feed type: 1 Fresh water: 1 Virus detected in rotifer culture 1
11 Number of crashes/year Crash Number of hatcheries < > 10 3 No relation between size of hatchery and number of crashes
12 Link between genotype and number of crashes? Crash < > 10 Genotype B. plicatilis s.s. 1 1 Austria 1 Cayman 5 Nevada 2 Mix Nevada-Cayman 1 Mix plicatilis- Cayman- Manjavacas All Cayman cultures are situated in the Mediterranean. 1
13 Mass cultures Feeding method: Manually: 8 hatcheries Continuously: 6 hatcheries Mixture: 3 Artificial diet: 5 Mixture: 5 Artificial diet: 1 Crash /year Mixture Art. diet Crash /year Mixture Art. diet < < > >
14 Conclusion: Too few data to point out a reason for crashes Most probably a multi-factorial problem
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