Setting up group models Part 1 NITP, 2011
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1 Setting up group models Part 1 NITP, 2011
2 What is coming up Crash course in setting up models 1-sample and 2-sample t-tests Paired t-tests ANOVA! Mean centering covariates Identifying rank deficient matrices
3 Recall GLM is flexible One Sample T Test ANOVA Two sample T Test Paired T test What do the models look like?
4 1-Sample T Test Overall mean
5 2-Sample T Test Mean group 1 Mean group 2
6 2-Sample T Test OR
7 Understanding a model If you re unsure about a model or the contrasts Plug in numbers Look at graphs (fmri data) Always ask yourself if your model is doing what you want it to
8 For example For the 2 sample T test Set Then G1=8 and G2=3 So is the mean of group 2 and is the difference between the groups What are the contrasts to test Mean of G2 Mean of G1 G1-G2
9 For example For the 2 sample T test Set Then G1=8 and G2=3 So is the mean of group 2 and is the difference between the groups What are the contrasts to test Mean of G2 Mean of G1 G1-G2
10 For example For the 2 sample T test Set Then G1=8 and G2=3 So is the mean of group 2 and is the difference between the groups What are the contrasts to test Mean of G2 Mean of G1 G1-G2
11 Paired T Test A common mistake is to use a 2-sample t test instead of a paired test Tire example Automobile Tire A Tire B sample T test p=0.58 Paired T test p<0.001
12 Why so different?
13 Why so different? Mean A Mean B
14 Why so different? Difference is OK
15 Why so different? Residuals are HUGE!
16 Paired T Test Adjust for the mean of each pair
17 Paired T Test Mean A Mean B
18 Paired T Test Difference is same Residual variance much smaller
19 Paired T Test GLM Difference Mean of each pair
20 ANOVA 1-way ANOVA 2-way ANOVA B A
21 Modeling ANOVA with GLM Cell means model Model a mean for each cell B A
22 Modeling ANOVA with GLM Factor effects model Model each factor as a set of regressors One regressor for overall mean, other regressors describe how factor effects relate to the overall mean
23 Modeling ANOVA with GLM Factor effects model Model the overall mean and have regressors for each factor Hypothesis tests from this model correspond to standard ANOVA hypotheses Eg, if group (2 levels) and stimulus type (3 levels) are modeled you can test for a main group effect, main stimulus type effect and interaction effect
24 1 Way ANOVA - Cell Means
25 1 Way ANOVA - Cell Means
26 1 Way ANOVA - Cell Means
27 1 Way ANOVA - Cell Means
28 1 Way ANOVA factor effects Always start with overall mean (column of 1s) Number of regressors for a specific factor is number of levels 1 Why? If I know the mean of 4 numbers is 10, then I only need to know 3 of the numbers to figure out what the fourth is.
29 1 Way ANOVA factor effects Always start with overall mean (column of 1s) Number of regressors for a specific factor is number of levels 1 Why? If I know the sum of 4 numbers is 10, then I only need to know 3 of the numbers to figure out what the fourth is.
30 1 Way ANOVA - Factor Effects In general # of regressors for a factor = # levels-1 Factor with 4 levels
31 1 Way ANOVA - Factor Effects In general # of regressors for a factor = # levels-1 Factor with 4 levels Note: I m assuming a balanced design!!
32 1 Way ANOVA - Factor Effects mean
33 1 Way ANOVA - Factor Effects mean
34 1 Way ANOVA - Factor Effects Mean g1 Mean g2 Mean g3 Mean g4
35 1 Way ANOVA - Factor Effects Mean g1 Mean g2 Mean g3 Mean g4
36 1 Way ANOVA - Factor Effects Mean g1 Mean g2 Mean g3 Mean g4
37 1 Way ANOVA - Factor Effects Mean g1 Mean g2 Mean g3 Mean g4
38 1 Way ANOVA - Factor Effects Mean g1 Mean g2 Mean g3 2 3 Mean g4
39 1 Way ANOVA - Factor Effects mean Test main effect of factor A? c =
40 1 Way ANOVA - Factor Effects
41 1 Way ANOVA - Factor Effects
42 1 Way ANOVA - Factor Effects
43 1 Way ANOVA - Factor Effects
44 2 Way ANOVA (3x2)
45 2 Way ANOVA (3x2) B1 B2 A1 A2 A3 No effect means the marginals would be the same Null: A1=A2=A3 equivalently A1-A3=0 and A2-A3=0
46 2 Way ANOVA (3x2)
47 2 Way ANOVA (3x2)
48 2 Way ANOVA (3x2)
49 2 Way ANOVA (3x2) B1 B2 A1 A2 A3 No effect means the lines would be parallel
50 2 Way ANOVA (3x2) B1 B2 A1 A2 A3 No effect means the lines would be parallel A1B1-A1B2=A2B1-A2B2=A3B1-A3B2
51 2 Way ANOVA (3x2) B1 B2 A1 A2 A3 No effect means the lines would be parallel A1B1-A1B2-A3B1+A3B2 = A2B1-A2B2-A3B1+A3B2 = 0
52 2 Way ANOVA (3x2)
53 By the way If you feel confused by that last example, that was my goal!
54 2 Way ANOVA - Factor Effects Recall for factor effects, a factor with n levels has regressors set up like A has 3 levels, so 2 regressors B has 2 levels, so 1 regressor
55 2 Way ANOVA - Factor Effects A B AB
56 2 Way ANOVA - Factor Effects
57 2 Way ANOVA - Factor Effects
58 2 Way ANOVA - Factor Effects
59 2 Way ANOVA - Factor Effects
60 2 Way ANOVA - Factor Effects
61 2 Way ANOVA - Factor Effects
62 For more examples The FSL folks have a bunch of great examples Check the FSL help list regularly Subscribe at jiscmail Often others have already asked your questions!
63 Mean centering covariates Why do we mean center? When should we mean center? What does it do to the parameter estimate interpretation?
64 Single group with covariate You have a single group of subjects and you also have measured age. You would like to see if there is an age effect. What would the model look like? What contrasts would you specify for the age effect? Can you still obtain the overall mean from this model?
65 Use age Use demeaned age Both models will give exactly the same result for C2, but C1 will be different.
66 Simulated data
67 Summary of mean centering Only really necessary if you want your PE of column of 1s to be the overall mean Often people have rounding errors after demeaning. Double-check this when you do it.
68 Two groups with continuous covariate We have two groups and a confounding covariate (depression). Our primary interest is in the difference of means between the two groups. What is the model to simply test the difference in means If I wanted to make sure this difference wasn t due to between group differences in depression, what would that model look like? What are some restrictions of this model?
69 Do not demean the confounding measure within group This removes any confounding effect the measure might have
70 Why you shouldn t demean within group What if this is what your data look like? Difference in means is clearly due to range of X sampled, not the group membership
71 Will mean centering across all subjects change anything?
72 Will mean centering across all subjects change anything? Nope!
73 Testing the interaction Same as previous example, but you d like to know if the relationship with depression score differs between your groups What would the design matrix and contrasts look like?
74 Design matrix No mean centering!
75 What contrasts make sense? Do comparisons of means make any sense? What is interesting about a significant interaction?
76 Compare means with a significant interaction? For what value of depression is the difference in means interesting?
77 What is interesting when there s an interaction effect?
78 Mean centering when testing an interaction It doesn t make much sense Difference between means varies by continuous covariate when there s an interaction effect Testing the mean difference in general doesn t make sense Only interesting contrast tests difference in slope for continuous covariates between groups
79 Mean centering guide
80 Important assumption That the effect tested is linear! Relationship between BOLD activation and depression score is linear for both groups If your continuous covariate is significantly different between your groups, you must be very careful with interpretation
81 When trends are linear, it doesn t matter where I sample age, I get the same slope
82 When trends are linear, it doesn t matter where I sample age, I get the same slope
83 When trends are linear, it doesn t matter where I sample age, I get the same slope
84 With a nonlinear trend, the trend may be the same in both groups, but sampling different ranges show different trends
85 With a nonlinear trend, the trend may be the same in both groups, but sampling different ranges show different trends
86 With a nonlinear trend, the trend may be the same in both groups, but sampling different ranges show different trends
87 Rank deficient designs I highly recommend becoming an expert on spotting rank deficient design matrices Don t the FSL list to ask why you got the error message at least one EV is (close to) a linear combination of the others
88 Rank deficient designs I highly recommend becoming an expert on spotting rank deficient design matrices Don t the FSL list to ask why you got the error message at least one EV is (close to) a linear combination of the others (the answer to the problem is in the error message!!)
89 Rank deficient One column is a linear combination of some of the other columns Linear combination of one set of columns equals a linear combination of another set of columns
90 Rank deficient
91 Rank deficient
92 Rank deficient?
93 Rank deficient?
94 That s it! Next lecture we ll look at more models. If you have a design that has you stumped, let me know what your data look like and we can all try to figure it out!
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