A. Preparation The laboratory exercises for each laboratory must be read before the laboratory period.

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1 #1 - Objectives: Identify the locations of important safety information and equipment; Understand how to properly use safety equipment in the laboratory; Understand the importance of being prepared for the laboratory; Understand your rights and responsibilities in the laboratory; Sign the agreements for Safety Rules and Rules for Eye Protection for the STEM Division at ; Review the parts, use, and storage of the microscope, I. Safety Training A. Preparation The laboratory exercises for each laboratory must be read before the laboratory period. 1) You must wear your gloves AND safety glasses during all exercises involving laboratory equipment, chemicals, glassware and preserved specimens. 2) Use extra caution if you need to use your scalpel or scissors. 3) Ask the instructor for supervision when changing your scalpel blade. 4) DO NOT touch anything but your equipment, instruments, specimens, etc. with your gloves on (ie. Not your laboratory manual or the computers). - Prepare your reference materials before beginning the exercise - Remove your gloves if you need to make notes, turn pages, etc. There are two reasons to wear gloves: i) to protect yourself and others from harmful chemicals (do not contaminate your work area) ii) to prevent contamination of the chemicals 5) Check the location of safety materials within the laboratory. 6) *Always bring: safety glasses, laboratory exercises, textbook, GLOVES (several pairs!), dissection kit, unruled paper, pencil AND Proper Shoes. You are always responsible for having these materials. B. Laboratory Materials / Clean up. 1) The laboratory is to be left the same or cleaner than when you entered it. 2) You must disinfect your bench BEFORE you put any of your own materials on it. You do not want to contaminate your things. 3) All materials used by yourself and / or your group are to be put away properly before leaving. Failing to do so may result in lost points for the laboratory. Page1

2 4) Your equipment and bench should be cleaned and disinfected before leaving the laboratory. 5) You must take extra care not to clog the sinks. All large debris must be disposed of in the appropriate garbage bin before the tray is washed. Utilize the strainers and check for clogging. Use the appropriate waste containers. If you are not sure, ask your instructor. 6) You should disinfect your laboratory aprons before AND after using them. 7) You must disinfect your bench AFTER you are finished. 8) When you are completely cleaned up the last thing you MUST do before you leave is wash your hands. For further information on emergency procedures please go to Delagate: C. Location of Safety Resources Nearest telephone: Emergency Telephone Numbers Emergency exits: Fire extinguisher: Emergency Shower: First aid kit: Eyewash station: Material safety data sheets: Fire alarm location: Additional Safety Instructions Microscope / station # D. STEM Division Student Biology Rules and Rules for Eye Protection You must read the following pages and understand your role in maintaining safety in the laboratory. Then sign both the Safety Rules and the Eyewear Rules and hand them in to your instructor. You must understand that you Will NOT be allowed to participate in the laboratory exercises without proper safety equipment or proper adherence to safety regulations in the laboratory and will be asked to leave the laboratory. Page2

3 DELAWARE COUNTY COMMUNITY COLLEGE DIVISION OF SCIENCE, TECHNOLOGY, ENGINEERING AND MATHEMATICS STUDENT BIOLOGY LABORATORY SAFETY RULES Purpose: It is the intention of the Delaware County Community College to provide its students, staff, and faculty with a safe laboratory environment. Since you will be performing laboratory activities that require the use of potentially dangerous equipment and/or materials, this list of Student Rules MUST be followed at all times. Your Instructor will explain these Rules to you BEFORE you perform your first laboratory activity. You will then sign one copy to be kept on file. You will also be given a second copy to keep as a constant reminder of the Rules and of your obligation to follow them. General Rules: 1. Be prepared for your work in the laboratory. Read all assigned procedures thoroughly before entering the laboratory. If you do not understand a direction or part of a procedure, ask your Instructor before proceeding. 2. Perform only those laboratory activities authorized by your Instructor. Never do anything in the laboratory that is not called for in the laboratory procedures or by your Instructor. 3. Horseplay, practical jokes, and pranks are dangerous, and are prohibited in the laboratory. 4. No student may enter or work in the laboratory without an Instructor present. 5. When first entering the laboratory, do not touch any equipment, chemicals, or other materials until you are instructed to do so. 6. Do not eat food, drink beverages, chew gum or tobacco, or apply cosmetics in the laboratory. 7. Any time chemicals, microorganisms, preserved specimens, heat, or glassware are used, students must wear approved laboratory eyewear that meets ANSI Z87.1 Standards, and contact lenses should not be worn. Approved laboratory eyewear is available for purchase in the Bookstore. You have been given a separate sheet that covers in detail our Rules Concerning Eye Protection in the Laboratory. 8. Dress properly during a laboratory activity. Long hair, dangling jewelry, and loose or baggy clothing are a hazard in the laboratory. Long hair must be tied back and dangling jewelry and loose or baggy clothing must be secured. Shoes must completely cover the foot. Sandals are not allowed. Laboratory aprons have been provided for your use and should be worn during laboratory activities. 9. Students are never permitted in the science storage rooms or preparation areas unless given specific permission by their Instructor. 10. Observe good housekeeping practices. Work areas should be kept clean and organized at all times. Bring only your laboratory instructions, worksheets, and/or reports to the work area. Other materials (books, purses, backpacks, etc.) should not be placed on the laboratory benches. 11. Keep your hands away from your face, eyes, mouth, and body while using chemicals, microorganisms, and preserved, living, or nonliving specimens. Wash your hands before and after performing all laboratory activities. Biology Page3

4 12. Do not touch, taste, or smell any chemicals unless specifically instructed to do so. The proper technique for smelling chemical fumes will be demonstrated to you. 13. Make sure your hands are completely dry before touching any electrical plug or device. 14. When inserting or removing an electrical plug from a socket, grasp the plug rather than the cord. 15. Report damaged electrical equipment to your Instructor immediately. Look for such things as frayed cords, exposed wires, or loose connections. Do not use damaged electrical equipment. 16. Do not leave ongoing laboratory activities unattended. You will be assigned a laboratory station at which to work. Do not distract other students or interfere with the laboratory activities of others. 17. Be alert and proceed with caution at all times in the laboratory. Notify your Instructor immediately of any unsafe conditions you observe. 18. Report any accident (spill, breakage, etc.) or injury (cut, burn, etc.) to your Instructor immediately, no matter how trivial it may appear. If the Instructor is unable to respond, then contact the Nurse and/or Security for assistance. 19. Know the location and purpose of the Material Safety Data Sheets (MSDS) for the chemicals used in the laboratory. Ask your Instructor if you need help in understanding them. 20. If you are or think you might be pregnant, if you have a lowered immune response, or if you have any other health concerns, consult with your physician before performing any of the laboratory activities. A list of chemicals and/or microorganisms used in the course will be provided upon request. 21. Know the locations and operating procedures of all safety equipment including eyewash stations, safety shower, fire extinguishers, fire blanket, and spill kits. Know where the fire alarm and the exits are located. 22. Know what to do during a building evacuation. Specific Rules for the Biology Laboratory: 23. Handle all living specimens in a humane manner. 24. Any time bacteria cultures or preserved specimens are handled, in addition to approved laboratory eyewear that meets ANSI Z87.1 Standards, students must also wear gloves and aprons. 25. When using knives and other sharp instruments, always carry with their tips and points pointing down and away from you and others. Always cut away from your body. Never try to catch a falling sharp instrument. Grasp sharp instruments only by the handles. 26. Properly dispose of all biological and chemical wastes, as directed by your Instructor. Do not place into the regular trash receptacles. 27. Properly dispose of all disposable instruments and wearing apparel, as directed by your Instructor. Do not place into the regular trash receptacles. Biology Page4

5 28. Properly dispose of all materials (toothpicks; cotton swabs; microscope slides & coverslips; paper towels; etc.) that have come into contact with any human body fluids (saliva; mucous from the tongue, throat, or inside the cheek; blood; etc.), as directed by your Instructor. Do not place into the regular trash receptacles. 29. Never use mouth suction to fill a pipet. Use a rubber bulb or a pipet pump. 30. If a chemical splashes onto your skin or face, immediately flush with running water from a sink. Use a safety shower if needed, or an eyewash station if the chemical got into your eyes. Notify your Instructor immediately. 31. Examine glassware before each use. Never use chipped, broken, or dirty glassware. 32. Never handle broken glassware with your bare hands. Use a brush and dustpan to clean up the glass, and then place into a designated disposal container. 33. Exercise care when using a gas burner or a hot plate. Keep at a safe distance, and do not reach over one that is being used. Turn the burner or hot plate off when not in use. 34. Heated glass or metal remains very hot for a long period of time, even after it appears to be cool. Allow it to cool completely before you pick it up with your bare hands, or else use tongs or heat-protective gloves. Agreement: I, (print full name) have read and agree to follow the Student Rules as explained to me by my Instructor and as set forth in this document. I realize that I must follow these Rules to ensure my own safety, as well as the safety of my fellow students, my Instructor, and other students, staff, and faculty. I also agree to follow any additional oral or written instructions that my Instructor may provide relative to a specific laboratory activity or situation. I am aware that any violation of these Rules on my part may result in my being asked to leave the laboratory, receiving a failing grade for the activity, and/or administrative withdrawal from the course. Student Signature Date Course & Section Number Biology Page5

6 DELAWARE COUNTY COMMUNITY COLLEGE DIVISION OF SCIENCE, TECHNOLOGY, ENGINEERING AND MATHEMATICS RULES CONCERNING EYE PROTECTION IN THE LABORATORY General Rules: Any time chemicals, microorganisms, preserved specimens, heat, or glassware are used, students must wear approved laboratory eyewear that meets ANSI Z87.1 Standards, and contact lenses should not be worn. Approved laboratory eyewear is available for purchase in the Bookstore. Any violation of this Rule may result in the student being asked to leave the laboratory, receiving a failing grade for the activity, and/or administrative withdrawal from the course. Approved Laboratory Eyewear: All laboratory eyewear must meet ANSI Z87.1 Standards. Since normal prescription eyeglasses do not provide the same level of protection as required by the ANSI Standards, they can not be used instead of laboratory eyewear, although they may be worn under laboratory goggles. The type of laboratory eyewear that is used depends upon the nature of the hazards that will be encountered. When eyewear is required, the following will apply. Chemistry: All Other Disciplines: Chemical splash goggles are required. Either safety glasses with side shields or chemical splash goggles are required, depending upon the nature of the laboratory activity. Contact Lenses: Any time chemicals, microorganisms, preserved specimens, heat, or glassware are used, contact lenses should not be worn. If the student also owns prescription eyeglasses, they should be worn at these times instead of the contact lenses, with laboratory goggles covering them. The reason for this Rule is that there are unique dangers associated with the wearing of contact lenses in the laboratory. These include: Should a chemical splash to the eye occur, the chemical could be held under the contact lens and against the surface of the cornea, possibly causing permanent eye damage. Involuntary spasm of the eyelid, and the panicked nature of the victim who has the chemical splashed in his/her eye make removal of the contact lens virtually impossible. By the time the lens is removed, irreversible damage already may have occurred. In a situation where the victim is unconscious, people attempting to irrigate the victim s eyes may be unaware that the victim wears contact lenses. Given these dangers, if a student still chooses to wear contact lenses when chemicals, microorganisms, preserved specimens, heat, or glassware are used, OSHA, ANSI (American National Standards Institute), and the ACS (American Chemical Society) all agree that the wearers of contact lenses should wear appropriate eye protection, which is generally defined to be non-ventilated chemical splash goggles. Agreement: I, (print full name) have read and agree to follow the Rules Concerning Eye Protection in the Laboratory as explained to me by my Instructor and as set forth in this document. I realize that I must follow these Rules to ensure my own safety. I also agree to follow any additional oral or written instructions that my Instructor may provide relative to a specific laboratory activity or situation. I am aware that any violation of these Rules on my part may result in my being asked to leave the laboratory, receiving a failing grade for the activity, and/or administrative withdrawal from the course. Student Signature Date Course & Section Number 0904 Page6

7 35. Properly dispose of all materials (toothpicks; cotton swabs; microscope slides & coverslips; paper towels; etc.) that have come into contact with any human body fluids (saliva; mucous from the tongue, throat, or inside the cheek; blood; etc.), as directed by your Instructor. Do not place into the regular trash receptacles. 36. Never use mouth suction to fill a pipet. Use a rubber bulb or a pipet pump. 37. If a chemical splashes onto your skin or face, immediately flush with running water from a sink. Use a safety shower if needed, or an eyewash station if the chemical got into your eyes. Notify your Instructor immediately. 38. Examine glassware before each use. Never use chipped, broken, or dirty glassware. 39. Never handle broken glassware with your bare hands. Use a brush and dustpan to clean up the glass, and then place into a designated disposal container. 40. Exercise care when using a gas burner or a hot plate. Keep at a safe distance, and do not reach over one that is being used. Turn the burner or hot plate off when not in use. 41. Heated glass or metal remains very hot for a long period of time, even after it appears to be cool. Allow it to cool completely before you pick it up with your bare hands, or else use tongs or heat-protective gloves. Agreement: I, (print full name) have read and agree to follow the Student Rules as explained to me by my Instructor and as set forth in this document. I realize that I must follow these Rules to ensure my own safety, as well as the safety of my fellow students, my Instructor, and other students, staff, and faculty. I also agree to follow any additional oral or written instructions that my Instructor may provide relative to a specific laboratory activity or situation. I am aware that any violation of these Rules on my part may result in my being asked to leave the laboratory, receiving a failing grade for the activity, and/or administrative withdrawal from the course. Student Signature Date Course & Section Number Biology Page7

8 DELAWARE COUNTY COMMUNITY COLLEGE DIVISION OF SCIENCE, TECHNOLOGY, ENGINEERING AND MATHEMATICS RULES CONCERNING EYE PROTECTION IN THE LABORATORY General Rules: Any time chemicals, microorganisms, preserved specimens, heat, or glassware are used, students must wear approved laboratory eyewear that meets ANSI Z87.1 Standards, and contact lenses should not be worn. Approved laboratory eyewear is available for purchase in the Bookstore. Any violation of this Rule may result in the student being asked to leave the laboratory, receiving a failing grade for the activity, and/or administrative withdrawal from the course. Approved Laboratory Eyewear: All laboratory eyewear must meet ANSI Z87.1 Standards. Since normal prescription eyeglasses do not provide the same level of protection as required by the ANSI Standards, they can not be used instead of laboratory eyewear, although they may be worn under laboratory goggles. The type of laboratory eyewear that is used depends upon the nature of the hazards that will be encountered. When eyewear is required, the following will apply. Chemistry: All Other Disciplines: Chemical splash goggles are required. Either safety glasses with side shields or chemical splash goggles are required, depending upon the nature of the laboratory activity. Contact Lenses: Any time chemicals, microorganisms, preserved specimens, heat, or glassware are used, contact lenses should not be worn. If the student also owns prescription eyeglasses, they should be worn at these times instead of the contact lenses, with laboratory goggles covering them. The reason for this Rule is that there are unique dangers associated with the wearing of contact lenses in the laboratory. These include: Should a chemical splash to the eye occur, the chemical could be held under the contact lens and against the surface of the cornea, possibly causing permanent eye damage. Involuntary spasm of the eyelid, and the panicked nature of the victim who has the chemical splashed in his/her eye make removal of the contact lens virtually impossible. By the time the lens is removed, irreversible damage already may have occurred. In a situation where the victim is unconscious, people attempting to irrigate the victim s eyes may be unaware that the victim wears contact lenses. Given these dangers, if a student still chooses to wear contact lenses when chemicals, microorganisms, preserved specimens, heat, or glassware are used, OSHA, ANSI (American National Standards Institute), and the ACS (American Chemical Society) all agree that the wearers of contact lenses should wear appropriate eye protection, which is generally defined to be non-ventilated chemical splash goggles. Agreement: I, (print full name) have read and agree to follow the Rules Concerning Eye Protection in the Laboratory as explained to me by my Instructor and as set forth in this document. I realize that I must follow these Rules to ensure my own safety. I also agree to follow any additional oral or written instructions that my Instructor may provide relative to a specific laboratory activity or situation. I am aware that any violation of these Rules on my part may result in my being asked to leave the laboratory, receiving a failing grade for the activity, and/or administrative withdrawal from the course. Student Signature Date Course & Section Number 0904 Page8

9 II. Microscope Review A. Introduction The microscope is a delicate and expensive piece of equipment that needs proper care so that it may function well for you and all the other students. Therefore, it must be handled and stored properly. You should already know the parts of the microscope and have a basic understanding of the function of each one. Figure 1 reviews the microscope parts. Figure 1a Figure 1b B. Microscope usage. How to handle and turn on the microscope: 1) Always carry it by the ARM with one hand on the BASE, always using both hands. 2) Plug in the microscope. 3) Turn on the microscope using the on / off switch at the back. 4) Make sure that you can see that the illuminator is on. - if it is not, adjust the voltage regulator or the iris diaphragm, or ask your instructor C. How to focus on a specimen: 1) Place the slide on the stage. 2) Make sure that the scanning (4x) objective is in place. 3) Using the coarse adjustment knob, adjust the stage to its highest position (closest to the specimen). With the scanning objective you cannot hit the slide or harm the objective. Page9

10 4) Using the mechanical stage place the specimen in the path of light. 5) Looking through the ocular, first use the coarse adjustment knob to bring the specimen in view. The ocular is usually 10-15x. Make note of your ocular magnification. 6) While focusing, it may be necessary to move the mechanical stage to bring the specimen into the field of view. 7) Use the fine adjustment knob to focus more clearly on the specimen. 8) Only now can you move up to the next objective (low power 10x). 9) Repeat steps 7 and 8 to move to the 40x (high dry) objective. *Remember to use the fine adjustment knob only for both the 10x and 40x objectives. Total magnification = ocular magnification x objective magnification. Microscope Use Check: Demonstrate that you can operate the microscope by showing your instructor that your slide is focused on the specimen using the 40x objective. Have your instructor check and initial the laboratory report that you have completed this task. D. How to put away the microscope: 1) Remove the slide and put it back in the slide tray. 2) Center the stage arm so that it does not protrude over the edge of the stage. 3) Place the voltage regulator to the lowest level. 4) Lower the stage fully. 5) Turn off the microscope. 6) Put the scanning objective into place. 7) Secure the power cord properly. 8) Put the cover on the microscope and put it away in its proper location as indicated by your instructor. Microscope Storage Check: Demonstrate that you can properly prepare the microscope for storage by showing your instructor the microscope before putting it away. Have your instructor check and initial the laboratory report that you have completed this task. F. Slides: 1) Be careful when transporting slides. Do not place slides on the bench or pile them up together. 2) Return slides to their correct location as indicated by your instructor. Page10

11 Laboratory Report Name: Safety Quiz 1. Why should you read your Laboratory Manual? 2. Name one thing an MSDS contains? 3. What does MSDS stand for?: 4. Why do you clean your bench-top BEFORE you start your laboratory activities? 5. Name one reason you should wear gloves. 6. What goggles are approved? 7. What type of footwear CANNOT be worn? 8. May you bring drinks into the laboratory? 9. Where is the emergency shower? 10. If you have ANY type of accident, who do you tell? 11. How do you know where to dispose of hazardous materials? 12. May you participate in the laboratory activities without ALL of the appropriate apparel or safety equipment? 13. Can regular glasses or sunglasses substitute for safety glasses? 14. May you perform laboratory activities that are not in the manual? 15. What do you do before leaving the laboratory? Microscope Use Check: Student can properly focus a specimen Microscope Storage Check: Student can properly prepare the microscope for storage Page11