Applied Biosystems 3400 DNA Synthesizer

Transcription

1 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 3, :09 pm, SPG_00_Title.fm

2 Copyright 2003, Applied Biosystems. All rights reserved. For Research Use Only. Not for use in diagnostic procedures. Information in this document is subject to change without notice. Applied Biosystems assumes no responsibility for any errors that may appear in this document. This document is believed to be complete and accurate at the time of publication. In no event shall Applied Biosystems be liable for incidental, special, multiple, or consequential damages in connection with or arising from the use of this document. NOTICE TO PURCHASER: PLEASE REFER TO THE Applied Biosystems 3400 DNA Synthesizer User Guide FOR LIMITED LABEL LICENSE OR DISCLAIMER INFORMATION. TRADEMARKS: Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the U.S. and certain other countries. AB (Design) and Applera are trademarks of Applera Corporation or its subsidiaries in the U.S. and certain other countries. All other trademarks are the sole property of their respective owners. Part Number Rev. B 04/2003 April 3, :09 pm, SPG_00_Title.fm

3 Contents Chapter 1 Introduction Overview About Preinstallation About Safety About Chemical Safety Chapter 2 Site Preparation Before You Start Preinstallation Checklists Items Shipped with This Instrument Laboratory Safety Requirements Laboratory Space Required Laboratory Ventilation Requirements Laboratory Environmental Requirements General Instrument Safety Electrical Requirements Pressurized Gas and Regulator Requirements Safety and Electromagnetic Compatibility (EMC) Standards Chapter 3 Chemical Safety Overview Material Safety Data Sheets Overview Hazardous Chemicals Hazardous Waste Waste Profiles Chapter 4 Instrument Safety Overview Instrument Labeling Safety Alert Symbols Safety Alert Symbols (French) Safety Alert Symbols (German) Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SitePrepGuide_TOC.fm iii

4 Safety Alert Symbols (Italian) Safety Alert Symbols (Portugese) Safety Alert Symbols (Spanish) Safety Alert Symbols (Chinese) Safety Alert Symbols (Japanese) Safety Alert Symbols (Korean) Safety Alert Symbols (Thai) Input/Output Connections Gas Safety Appendix A Acronyms and Abbreviations Acronyms and Abbreviations Used in Material Safety Data Sheets A-1 Appendix B Technical Support and Training How to Obtain Services and Support B-1 iv Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SitePrepGuide_TOC.fm

5 Introduction 1 1 Overview About the Site Preparation and Safety Guide In This Guide The Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide (PN ) is sent to all customers who have purchased an Applied Biosystems instrument. This guide provides the information you need to fully prepare your site for the arrival and installation of the instrument. Complete preparation helps ensure a smooth installation process, as well as correct and safe instrument operation. This guide for the Applied Biosystems 3400 DNA Synthesizer contains the following chapters and appendixes: Chapter Title Introduction Site Preparation Chemical Safety Instrument Safety Appendix Title Acronyms and Abbreviations Technical Support and Training Description Provides orientation to this guide and information about ordering Material Safety Data Sheets (MSDSs) Provides installation requirements and the preinstallation checklists Provides general chemical handling guidelines and instrument waste profiles (if any) Explains safety alert symbols on instrument and shows instrument input and output connections Description Explains terms used in MSDSs and in this guide Provides contact information for technical support and training Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 1-1 April 9, :25 pm, SPG_01_Intro.fm

6 Chapter 1 Introduction About Preinstallation Preparing Your Site Choosing a Location Preinstallation checklists start on page 2-2 of this guide. An Applied Biosystems employee will contact you to verify that everything on the checklists has been done before making an appointment for installation. When deciding where to put the instrument, keep in mind the following: You must be able to disconnect the main power supply to the instrument immediately if necessary. You must be able to comply with local, state/provincial, or national air quality regulations while venting the exhaust from this instrument. You must be able to provide easy access to all four sides of the instrument for servicing. About Safety Using the Instrument Correctly Documentation User Attention Words Use this instrument as specified by Applied Biosystems. If the instrument is used in a manner not specified by the manufacturer, the protection provided by the instrument may be impaired. Five user attention words appear in the text of all Applied Biosystems user documentation. Each word implies a particular level of observation or action as described below. Note: Calls attention to useful information. IMPORTANT! Indicates information that is necessary for proper instrument operation, accurate chemistry kit use, or safe use of a chemical. Indicates a potentially hazardous situation which, if not avoided, may result in minor or moderate injury. It may also be used to alert against unsafe practices. Indicates a potentially hazardous situation which, if not avoided, could result in death or serious injury. Indicates an imminently hazardous situation which, if not avoided, will result in death or serious injury. This signal word is to be limited to the most extreme situations. 1-2 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_01_Intro.fm

7 About Chemical Safety About Chemical Safety About MSDSs Some of the chemicals used with this instrument may be listed as hazardous by their manufacturer. When hazards exist, warnings are prominently displayed on the labels of all chemicals. Chemical manufacturers supply a current MSDS before or with shipments of hazardous chemicals to new customers and with the first shipment of a hazardous chemical after an MSDS update. MSDSs provide you with the safety information you need to store, handle, transport and dispose of the chemicals safely. Each time you receive a new MSDS packaged with a hazardous chemical, be sure to replace the appropriate MSDS in your files. CHEMICAL HAZARD. Be sure to familiarize yourself with the MSDSs before using reagents or solvents. Obtaining MSDSs You can obtain from Applied Biosystems the MSDS for any chemical supplied by Applied Biosystems. This service is free and available 24 hours a day. To obtain MSDSs: 1. Go to 2. In the Search field, type in the chemical name, part number, or other information that appears in the MSDS of interest. Select the language of your choice, then click Search. 3. Find the document of interest, right-click the document title, then select any of the following: Open To view the document Print Target To print the document Save Target As To download a PDF version of the document to a destination that you choose 4. To have a copy of a document sent by fax or , select Fax or to the left of the document title in the Search Results page, then click RETRIEVE DOCUMENTS at the end of the document list. 5. After you enter the required information, click View/Deliver Selected Documents Now. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 1-3 April 9, :25 pm, SPG_01_Intro.fm

8 Chapter 1 Introduction About Waste Profiles Chemical Hazard Warning Chemical Waste Hazard Warning Waste profile(s) are provided with this manual. They list the percentage compositions of the reagents within the waste stream at installation and the waste stream during a typical user application. These profiles assist users in planning for instrument waste handling and disposal, which must be in accordance with local, state/provincial, or national regulations. Read the waste profiles and all applicable MSDSs before handling or disposing of waste. IMPORTANT! Waste profiles are not a substitute for MSDS information. CHEMICAL HAZARD. Some of the chemicals used with Applied Biosystems instruments and protocols are potentially hazardous and can cause injury, illness, or death. Read and understand the material safety data sheets (MSDSs) provided by the chemical manufacturer before you store, handle, or work with any chemicals or hazardous materials. Minimize contact with chemicals. Wear appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing). For additional safety guidelines, consult the MSDS. Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (for example, fume hood). For additional safety guidelines, consult the MSDS. Check regularly for chemical leaks or spills. If a leak or spill occurs, follow the manufacturer s cleanup procedures as recommended on the MSDS. Comply with all local, state/provincial, or national laws and regulations related to chemical storage, handling, and disposal. CHEMICAL WASTE HAZARD. Wastes produced by Applied Biosystems instruments are potentially hazardous and can cause injury, illness, or death. Read and understand the material safety data sheets (MSDSs) provided by the manufacturers of the chemicals in the waste container before you store, handle, or dispose of chemical waste. Handle chemical wastes in a fume hood. Minimize contact with chemicals. Wear appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing). For additional safety guidelines, consult the MSDS. Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (for example, fume hood). For additional safety guidelines, consult the MSDS. After emptying the waste container, seal it with the cap provided. Dispose of the contents of the waste tray and waste bottle in accordance with good laboratory practices and local, state/provincial, or national environmental and health regulations. 1-4 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_01_Intro.fm

9 Site Preparation 2 2 Before You Start Preinstallation Operator Training Performance Verification Ordering Supplies Unpacking Before the Applied Biosystems 3400 DNA Synthesizer (PN ) is installed, the installation site must be prepared so that the instrument can be operated correctly and safely. Follow the requirements presented in Preinstallation Checklists on page 2-2, to simplify the installation procedure. Training of operators is one of the primary goals of installation. Personnel requiring training should set aside one uninterrupted day to work with the Applied Biosystems service representative. If this is not possible, please call Applied Biosystems Service Administration to reschedule the installation. Calibration of the instrument and verification of performance will be performed by an Applied Biosystems service representative during the installation. Before installation, be sure to contact Sales Administration or your sales representative to order the additional supplies necessary for the ongoing operation of the instrument. The chemicals shipped with this instrument will be completely consumed during the installation and initial testing of the instrument. Do not unpack instruments. Inspect instrument cartons and report any damage to your Applied Biosystems service representative. For information and instructions about unpacking the Installation Chemical Kit, see Items Shipped with This Instrument on page 2-5. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 2-1 April 9, :25 pm, SPG_02_SitePrep.fm

10 Chapter 2 Site Preparation Preinstallation Checklists About These Checklists Components Supplied by Applied Biosystems Use the following preinstallation checklists to ensure that all preparations are made for installing your instrument. All of the personnel and supplies on these checklists are required at the time of installation, but most of them are not supplied by Applied Biosystems. A service representative will contact you to confirm that everything is checked off before making an appointment for installation. The following components are supplied by Applied Biosystems. Check off the items after performing the actions. Date Confirmed Action Received instrument and inspected the crate. Verified that instrument, serial number, and system configuration, as shown on the packing list and in Items Shipped with This Instrument on page 2-5, are the same as ordered. Reported any discrepancies in instrument, serial number, or system configuration, or damage to the crate to your Applied Biosystems service representative. Read all sections of this 3400 DNA Synthesizer Site Preparation and Safety Guide. Read the MSDSs provided with the Installation Chemistry Kit. Unpacked and stored contents of the Installation Chemistry Kit, verifying the contents against the packing list in the kit or the list on page 2-5 of this guide. Verified the number of boxes received in the Packing (Installation or Accessory) Kit against the packing list provided in the kit. On-Site Personnel Check off the items below after confirming the following with your laboratory personnel. Date Confirmed Action Have available one uninterrupted day for in-lab training during installation. Designated one person to be the laboratory safety representative. This person is familiar with laboratory safety procedures, knows the location of all safety equipment, and must be available to the Applied Biosystems engineer while the engineer is at your facility. Designated one person to be present to assist the service engineer in lifting and/or positioning the instrument. 2-2 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_02_SitePrep.fm

11 Preinstallation Checklists Laboratory Facility Requirements Check off each item as you verify that the instrument location meets each of the requirements. Date Confirmed Requirement Safety Met requirements specified in Laboratory Safety Requirements on page 2-6. Instrument Location Verified a pathway from the receiving dock to the storage area (if applicable) to accommodate the instrument s 86.4-cm (34-in.) crated width. Verified a pathway from the storage area to the laboratory to accommodate the instrument s 66.0-cm (26-in.) width. Laboratory space is of correct dimensions to accommodate the system and its ventilation clearances, and is situated so that the instrument is accessible to the installer on all four sides. See Laboratory Space Required on page 2-7. Laboratory bench is of the correct dimensions and weight tolerance to accommodate the system and computer. See Laboratory Space Required on page 2-7. Situated the computer (if applicable) to allow for proper ergonomics during use. Ventilation and Waste A properly functioning ventilation system is within 2.4 m (8 ft) of the instrument. Provided a system for exhausting chemical vapor/gaseous instrument tubing line to a fume hood with a face velocity measurement of 100 linear ft/min (30 linear m/min) averaged over the face of the hood. The minimum velocity measured at any one point in the face of the hood should be 80 linear ft/min (24 linear m/min), with maximum velocity measured at 125 linear ft/min (38 linear m/min). If an instrument exhaust duct system is provided, ensure that the flow rate across the face of the duct is at least 100 linear ft/min (30 linear m/min) at the connection point of the waste exhaust line to the duct. Provided a system for instrument ventilation if connected to a laboratory fume hood with a face velocity measurement of 100 linear ft/min (30 linear m/min) averaged over the face of the hood. The minimum velocity measured at any one point in the face of the hood should be 80 linear ft/min (24 linear m/min), with maximum velocity measured at 125 linear ft/min (38 linear m/min). If an instrument exhaust duct system is provided, ensure that the flow rate across the face of the duct is at least 100 linear ft/min (30 linear m/min) at the connection point of the waste exhaust line to the duct. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 2-3 April 9, :25 pm, SPG_02_SitePrep.fm

12 Chapter 2 Site Preparation Date Confirmed Requirement Ensured that instrument ventilation and exhaust systems to outside air will meet all local, state/provincial, or national air quality regulations. Obtained permit if necessary. Met other laboratory ventilation requirements, as specified in Laboratory Ventilation Specifications on page 2-9. Established proper handling and disposal method(s) for hazardous chemical waste (if appropriate). Verified that room ventilation can accommodate instrument heat output of Btu/h (150 W). Verified that laboratory ventilation can accommodate other environmental requirements as specified in Laboratory Environmental Requirements on page Electrical A dedicated 1.5-kVA power line and ground is required for the system. A line conditioner or an uninterruptible power supply (UPS) on the power line is recommended. One standard power outlet is within 2.1 m (7 ft) of the instrument location, preferably near the back of the instrument. Met requirements specified in Electrical Requirements on page Equipment and Consumables Required You must supply the following items for installation. Check off each item as you verify its availability. Date Confirmed Item Required equipment specified in Pressurized Gas and Regulator Requirements on page 2-15 Safety glasses Lab coats Chemical-resistant disposable gloves Top-loading balance, accurate to ±0.01 g 2-4 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_02_SitePrep.fm

13 Items Shipped with This Instrument Items Shipped with This Instrument Summary List Do Not Move or Unpack Instrument The 3400 DNA Synthesizer is shipped as follows: 3400 DNA Synthesizer (PN ) in one crate 3400 DNA Synthesizer Installation Chemistry Kit (PN ) in one box 3400 DNA Synthesizer Installation Packing Kit (PN ) in one box Do not move or unpack instrument cartons. This protects you from liability if any damage occurred during shipping. Inspect instrument cartons, and report any damage to your Applied Biosystems service representative. PHYSICAL INJURY HAZARD. Do not move or unpack instrument cartons. This instrument is heavy. Any incorrect lifting or moving of the instrument can cause painful and sometimes permanent back injury. The instrument may tip over if moved or unpacked incorrectly, causing serious injury to persons in its path or damage to the instrument itself. Unpacking the instrument will also void your warranty with Applied Biosystems. Installation Chemistry Kit Unpack the 3400 DNA Synthesizer Installation Chemistry Kit upon receipt, and store the chemical as indicated in the table below. The chemical in this kit is completely consumed during the installation and initial testing of the instrument. Part Number Description Quantity Shipping Condition Storage Condition L bottles (empty) Acetonitrile, 4 L 2 Room Temperature Room Temperature Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 2-5 April 9, :25 pm, SPG_02_SitePrep.fm

14 Chapter 2 Site Preparation Laboratory Safety Requirements On-Site Representative Required Safety Equipment Applied Biosystems requests that a representative from your laboratory be in the vicinity and available to the Applied Biosystems engineer at all times while the engineer is at your facility. This person should be familiar with laboratory safety procedures and know the location of all the safety equipment. Your laboratory has specific safety practices and policies designed to protect laboratory personnel from potential hazards that are present. Applied Biosystems expects that all applicable safety-related procedures will be followed at all times. The following safety equipment must be available: Fire extinguisher It is your responsibility to provide appropriate fire extinguishers for use on or near Applied Biosystems equipment. The types and sizes of fire extinguishers shall be suitable for use on electrical and chemical fires as specified in currently prevailing codes, regulations and/or standards, and with the approval of the Fire Marshall or other authority having jurisdiction. The installation of appropriate fire extinguishers shall be in addition to other fire protection systems and not as a substitute or alternative to them. Eyewash Safety shower Eye and hand protection Adequate ventilation First-aid equipment Spill cleanup equipment Protection from any sources of hazardous chemicals, radiation (lasers, radioisotopes, contaminated equipment, radioactive wastes, and so forth.) and potentially infectious biological material that may be present in the area where Applied Biosystems engineer(s) will be working 2-6 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_02_SitePrep.fm

15 Laboratory Space Required Laboratory Space Required Dimensions and Weight The 3400 DNA Synthesizer has the following dimensions: Component Width Depth Height Weight 3400 DNA Synthesizer, crated 86.4 cm (34 in) 71.1 cm (28 in) 78.7 cm (31 in) 81.6 kg 180 lbs 3400 DNA Synthesizer, uncrated 66.0 cm (26 in) 45.7 cm (18 in) 50.5 cm (19.9 in) 42.9 kg 94.6 lbs 3400 DNA Synthesizer, with all required clearances 117 cm (46 in) 76.2 cm (30 in) cm (56 in) --- Instrument Location Assisting the Engineer Move the Instrument Plan on providing sufficient laboratory space for the instrument system as well as the following accommodations: 20.3 cm (8 in.) of clearance at the rear of the instrument for adequate ventilation. Access to all four sides of instrument for servicing. Do not block access to the rear of the instrument. Laboratory bench of correct dimensions and weight tolerance to accommodate the system. At least one person will be required to assist the service engineer place the instrument onto the laboratory bench. Do not unpack or move the instrument before the service engineer arrives to install it. PHYSICAL INJURY HAZARD. Do not attempt to lift the instrument or any other heavy objects unless you have received related training. Incorrect lifting can cause painful and sometimes permanent back injury. Use proper lifting techniques when lifting or moving the instrument. Two or three people are required to lift the instrument, depending upon instrument weight. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 2-7 April 9, :25 pm, SPG_02_SitePrep.fm

16 Chapter 2 Site Preparation Typical Laboratory Layout A typical laboratory layout is shown below. To non-chlorinated waste container To chlorinated waste container Power cord Ethernet cable Power cord Router 2 L Reagent containers 2 L 4 L 3400 instrument Computer optional, supplied by customer GR L non-chlorinated waste container 6 L chlorinated waste container Waste containers (stored under bench) 2-8 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_02_SitePrep.fm

17 Laboratory Ventilation Requirements Laboratory Ventilation Requirements Waste/Exhaust System Overview Laboratory Ventilation Specifications About the Waste/Exhaust System The waste system removes both liquid and gaseous waste from the instrument lines. The liquid wastes accumulate in waste containers. The gaseous waste is delivered via an exhaust line from the top of the waste containers to the laboratory ventilation system. The laboratory ventilation system(s) to this instrument must be operating properly whenever the instrument power is on, waste is in the waste container, or reagents are on the instrument. For the 3400 DNA Synthesizer, proper ventilation operation specifications include: A fume hood with a face velocity measurement of 100 linear ft/min (30 linear m/min) averaged over the face of the hood. The minimum velocity measured at any one point in the face of the hood should be 80 linear ft/min (24 linear m/min), with maximum velocity measured at 125 linear ft/min (38 linear m/min). An exhaust duct with the average flow rate across the face of the duct at least 100 linear ft/min (30 linear m/min). The waste/exhaust system is composed of two liquid waste lines, which come off the back of the instrument. One line is for non-chlorinated waste and goes to a 10-L (2.6-gal) polyethylene container. The other line is for chlorinated waste and goes to a 6-L (1.6-gal) polyethylene container. A vent line comes out of each waste container. These vent lines can be connected to a venting duct directly or tee d off before going to a vent duct for removing vapors. CHEMICAL STORAGE HAZARD. Never collect or store waste in a glass container because of the risk of breaking or shattering. Reagent and waste bottles can crack and leak. Each waste bottle should be secured in a lowdensity polyethylene safety container with the cover fastened and the handles locked in the upright position. Wear appropriate eyewear, clothing, and gloves when handling reagent and waste bottles. For more information on handling liquid waste, see Chapter 3. Connecting the Gaseous Waste Exhaust Line Follow these guidelines when connecting the tubing from the instrument s gaseous waste exhaust to your ventilation system: Use the shortest possible length and the straightest possible run of polypropylene tubing. Tubing length should not exceed 2.4 m (8 ft). Make sure that the tubing does not have low points that can trap residue or condensation. Fasten the tubing securely. Use polypropylene or Teflon fasteners. Do not use brass; it corrodes. Be careful not to puncture tubing. Place the tubing away from sources of potential damage, such as heat, flame, or points of contact (rubbing) with other objects. Place the tubing end as far as possible into the duct, canopy, or hood. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 2-9 April 9, :25 pm, SPG_02_SitePrep.fm

18 Chapter 2 Site Preparation Oncoming air movement through the duct or canopy should not face the open end of the tubing. Connecting the Liquid Waste Line About the System Vent Heat Production Connect the liquid waste lines from the instrument to the waste containers so that the lines drop vertically. Doing so prevents liquid and waste from accumulating and blocking the flow. The 3400 DNA Synthesizer does not emit enough heat to require a system vent. The thermal output of the instrument is Btu/h (150 W). Consult your facilities department regarding ventilation requirements for this level of heat output. Laboratory Ventilation System for a Canopy and/or Duct Building Air Intake Building air intake must not draw gaseous waste back into the building Instrument Exhaust Line (may be flexible duct) Duct Movement of Gaseous Waste Duct-Work Verify that ducts are constructed of materials that are compatible with vented waste. Do not simultaneously vent strong oxidizers, bases or other chemicals that are incompatible with gaseous waste. Maintain negative pressure in duct. Canopy Instrument System Vent Airflow Indicator Waste Bottle Exhaust Line Waste Line Tubing (or flexible duct) Face open end of tubing away from incoming air. Place tubing as far into the hood or canopy as possible. Use a straight-upward run of no more than 4.5 m (15 ft) of polypropylene tubing. Securely fasten tubing with polypropylene or Teflon fasteners, not brass (brass corrodes). Ventilation Operation Operate the laboratory ventilation system whenever the instrument power is on, waste is in the waste bottle, or reagents are on the instrument. This includes nights and weekends. Do not connect the instrument to a ductless hood or a system that purifies/filters air and returns it to the room Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_02_SitePrep.fm

19 Laboratory Ventilation Requirements Laboratory Ventilation System for a Fume Hood Fume Hood Airflow Indicator Building Air Intake Building air intake must not draw gaseous waste back into the building. Instrument or Waste Exhaust Line Fume Hood Verify that construction materials are compatible with vented waste. Do not simultaneously vent strong oxidizers, bases or other chemicals that are incompatible with gaseous waste. Locate hood away from air currents created by air-conditioning ducts, fans, windows, doors, or moving objects or people. Verify that hood meets local, state/provincial, or national standards. Post label at sash level that provides an average face-level airflow velocity of 30 linear m/min (100 linear ft/min). See Laboratory Ventilation Specifications on page 2-9. Waste Line Tubing Use no more than 4.5 m (15 ft) of polypropylene tubing. Securely fasten tubing with polypropylene or Teflon fasteners, not brass (brass corrodes). Ventilation Operation Operate the laboratory ventilation system whenever the instrument power is on, waste is in the waste bottle, or reagents are on the instrument. This includes nights and weekends. Do not connect the instrument to a ductless hood or a system that purifies/filters air and returns it to the room. Fume Hood Operation Follow these guidelines for fume hood operation and maintenance: Operate the fume hood whenever the instrument power is on, waste is in the waste container, or reagents are on the instrument. Use a fume hood that is constructed of materials that are compatible with the waste materials/chemicals being generated or exhausted. Locate the fume hood away from air currents generated by air conditioning ducts, fans, windows, doors, and moving equipment and persons. Locate the fume hood exhaust vent where gaseous waste cannot be drawn back into the building. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 2-11 April 9, :25 pm, SPG_02_SitePrep.fm

20 Chapter 2 Site Preparation Affix a sign or label to indicate the position of the fume hood sash that produces an average air flow of 100 linear ft/min face velocity. The minimum flow velocity at any point in the hood is 80 linear ft/min, and the maximum is 125 linear ft/min. Ensure that the fume hood meets all local, state/provincial, or national safety requirements. Have a safety professional or mechanical ventilation expert check and record air velocity a minimum of once a year. Inspect and maintain the exhaust system, including fans and motors, a minimum of once a year. Duct System Operation Waste Ventilation Warnings Follow these guidelines for duct system operation and maintenance: Operate the duct system whenever the instrument power is on, waste is in the waste container, or reagents are on the instrument. Use a duct system constructed of polyvinylidene fluoride (PVDF) tubing or other materials compatible with the waste material being generated. Do not allow the duct system to come into contact with strong oxidizers, bases, or other chemicals that are incompatible with gaseous waste. CHEMICAL WASTE HAZARD. Waste produced by Applied Biosystems instruments can be hazardous and can cause injury, illness, or death. Operate a vented instrument only if it is connected in accordance with all requirements. Operate the system venting gaseous waste whenever the instrument power is on, waste is in the waste container, or reagents are on the instrument. Handle all liquid, solid, and gaseous waste as potentially hazardous. See the MSDSs provided by the manufacturer(s) of chemicals used at your site for more information. Connect the exhaust line so that it leads to the fume hood or duct in a straight and upward direction. Low points will allow condensation to collect, preventing flow through the instrument. Do not connect the waste vent line to a ductless hood or to a system that purifies and filters air and returns it to the room. Always mix and prepare hazardous materials beneath an operating fume hood. Dispose of waste in accordance with all local, state/provincial, or national health and safety regulations and laws. Venting hazardous waste may require local, state/provincial, or national air permits Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_02_SitePrep.fm

21 Laboratory Environmental Requirements Laboratory Environmental Requirements Altitude Temperature and Humidity Pollution Emission/ Immunity Statement This instrument is for indoor use only and for altitudes not exceeding 2000 m (6500 ft) above sea level. The laboratory temperature should be between C (60 72 F). The instrument can tolerate maximum relative humidity of 80% for temperatures up to 31 C, decreasing linearly to 50% relative humidity at 40 C. Avoid placing the instrument adjacent to heaters, cooling ducts, or in direct sunlight. The installation category (overvoltage category) for this instrument is II, and it is classified as stationary equipment. The instrument has a pollution degree rating of 2 and may be installed in an environment that has nonconductive pollutants only. For our European customers, any product marked with the CE label meets the European EMC Directive 89/336/EEC and the Low Voltage Directive 72/23/EEC. This product meets Class B emission limits. General Instrument Safety PHYSICAL INJURY HAZARD. Use this instrument as specified by Applied Biosystems. Using this instrument in a manner not specified by applied Biosystems may result in personal injury or damage to the instrument. Electrical Requirements Power IMPORTANT! You must be able to disconnect the main power supply to the instrument immediately if necessary. The following table specifies the electrical operating range for the instrument in various parts of the world: Location Voltage (VAC) Frequency Amperage (A) Japan 100 ±10% 50/60 Hz ±1% <6.25 USA/Canada 120 ±10% 50/60 Hz ±1% <6.25 Europe (pre-1992) 220 ±10% 50/60 Hz ±1% <6.25 EC 230 ±10% 50/60 Hz ±1% <6.25 UK (pre-1992) %/ 10% 50/60 Hz ±1% <6.25 Australia %/ 10% 50/60 Hz ±1% <6.25 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 2-13 April 9, :25 pm, SPG_02_SitePrep.fm

22 Chapter 2 Site Preparation Power Line Electrical Outlets The electrical receptacle must have a dedicated 1.5 kva power line and ground or a 1.5 kva power line with a line conditioner or uninterruptible power supply (UPS). This instrument requires a Nema 5-15 receptacle in the USA. The electrical receptacle must be located within 2.5 m (8 ft) of the instrument rear panel. Do not use extension cords. Power Rating This instrument is rated for a maximum output of 540 W. Power Cords Grounding Power Line Regulator Voltage Spikes Power Outages In the USA, Canada, and Japan, the instrument is supplied with a detachable cord equipped with a standard three-prong plug. In Europe and Australia, the instrument is supplied with an detachable electrical cord equipped with a standard EC plug. Certain types of electrical noise are greatly exaggerated by poor or improper electrical ground connections. To prevent these problems, it is very important to have a dedicated line and ground between the instrument and building main electrical service. In areas where the supplied power is subject to voltage fluctuations exceeding ±10% of the nominal value (above), a power line regulator may be required. High or low voltages can have adverse effects on the electronic components of the instrument. Short-duration, high-voltage spikes often cause random failures in microprocessorcontrolled instrumentation. These spikes can be caused by other devices using the same power source (refrigerators, air conditioners, and centrifuges) or by outside influences such as lightning. A dedicated line and ground between the instrument and building main electrical service will prevent such problems. If your environment contains devices that are electrically noisy or you are in an area with frequent electrical storms, a line conditioner with a recommended capacity of at least 1.5 kva will enhance the reliability of your system. The instrument has been designed to pause from short periods of power outage (loss). To continue operation you must resume the run from the software, provided that the line voltage did not become excessively noisy before the outage. If you want increased protection during a power outage, install a UPS with a capacity of 2.0 kva Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_02_SitePrep.fm

23 Pressurized Gas and Regulator Requirements Pressurized Gas and Regulator Requirements Gas Cylinder You must supply the required gas cylinder and accessories for installation. This instrument requires one size 1-A argon gas cylinder that holds approximately 7.2 m 3 (257 ft 3 ) of gas when full. Use only prepurified argon of % or greater purity. An additional gas cylinder should be ordered in advance for the ongoing operation of the instrument. Damage to the instrument and its products can result from using impure argon, gases other than argon, or an inadequate amount of argon. EXPLOSION HAZARD. Pressurized gas cylinders are potentially explosive. Always cap the gas cylinder when it is not in use and attach it firmly to the wall or gas cylinder cart with approved brackets or chains. Pressure Regulator The argon tank requires a two-gauge regulator with a Compressed Gas Association (CGA) 580 argon cylinder adapter on the inlet side and a fitting that accepts a 6.35-mm (1/4-in) o.d. tube. The primary gauge ( psi; 0 25,000 kpa recommended) measures tank pressure. The secondary gauge (0 200 psi; kpa recommended) measures regulated pressure. The secondary gauge must allow regulation between psi. BOTTLE EXPLOSION HAZARD. Bottle explosion can cause severe physical injury. To prevent reagent bottles from becoming overpressurized and possibly exploding, the pressure regulator and the pressure relief valve must be in place and working properly. Failure of these two components will cause bottle overpressurization and bottle explosion. Attaching the Cylinder Attach the pressurized gas cylinder firmly to a wall or gas cylinder cart by means of approved straps or chains. EXPLOSION HAZARD. Pressurized gas cylinders are potentially explosive. Always cap the gas cylinder when it is not in use and attach it firmly to the wall or gas cylinder cart with approved brackets or chains. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 2-15 April 9, :25 pm, SPG_02_SitePrep.fm

24 Chapter 2 Site Preparation Safety and Electromagnetic Compatibility (EMC) Standards This section provides information on: U.S. and Canadian Safety Standards Canadian EMC Standards European Safety and EMC Standards Australian EMC Standards U.S. and Canadian Safety Standards Canadian EMC Standards European Safety and EMC Standards This instrument has been tested to and complies with standard UL , Safety Requirements for Electrical Equipment for Laboratory Use, Part 1: General Requirements. This instrument has been tested to and complies with standard CSA , Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part 1: General Requirements. This instrument has been tested to and complies with Canadian ICES-001, Issue 3: Industrial, Scientific, and Medical Radio Frequency Generators. Safety This instrument meets European requirements for safety (Low Voltage Directive 73/23/EEC). This instrument has been tested to and complies with standards EN , Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use, Part 1: General Requirements and EN , Particular Requirements for Laboratory Equipment for the Heating of Materials. EMC This instrument meets European requirements for emission and immunity (EMC Directive 89/336/EEC). This instrument has been tested to and complies with standard EN (Group 1, Class B), Electrical Equipment for Measurement, Control and Laboratory Use EMC Requirements. Australian EMC Standards This instrument has been tested to and complies with standard AS/NZS 2064, Limits and Methods Measurement of Electromagnetic Disturbance Characteristics of Industrial, Scientific, and Medical (ISM) Radio-frequency Equipment Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_02_SitePrep.fm

25 Chemical Safety 3 3 Overview In This Chapter This chapter contains general information about handling hazardous chemicals and waste. It also contains information for the Applied Biosystems 3400 DNA Synthesizer (PN ) about: Material safety data sheets (MSDSs) Hazardous waste produced (if any) during the installation procedure Hazardous waste produced during typical use of the instrument, if appropriate Applied Biosystems assumes that all operations in your laboratory will be conducted in accordance with safety practices detailed in the MSDSs for the chemicals used in your laboratory, and with all local, state/provincial, or national regulations. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 3-1 April 9, :25 pm, SPG_03_ChemSafety.fm

26 Chapter 3 Chemical Safety Material Safety Data Sheets Overview About MSDSs Some of the chemicals used with this instrument may be listed as hazardous by their manufacturer. When hazards exist, warnings are prominently displayed on the labels of all chemicals. Chemical manufacturers supply a current MSDS before or with shipments of hazardous chemicals to new customers and with the first shipment of a hazardous chemical after an MSDS update. MSDSs provide you with the safety information you need to store, handle, transport and dispose of the chemicals safely. Applied Biosystems strongly recommends that you replace the appropriate MSDS in your files each time you receive a new MSDS packaged with a hazardous chemical. CHEMICAL HAZARD. Be sure to familiarize yourself with the MSDSs before using reagents or solvents. Explanations of acronyms and abbreviations used in MSDSs can be found in Appendix A of this guide. Updating MSDSs Ordering MSDSs from Applied Biosystems Ordering MSDSs from Other Manufacturers Chemical manufacturers supply a current MSDS before or with shipments of hazardous chemicals to new customers, and with the first shipment of a hazardous chemical after an MSDS update. Replace the MSDSs in your files regularly so that the safety information is current. You can order free additional copies of MSDSs for chemicals manufactured or distributed by Applied Biosystems. See Obtaining MSDSs on page 1-3 for details. Applied Biosystems does not furnish MSDSs for chemicals used on this instrument that are not manufactured or distributed by Applied Biosystems. Contact the manufacturer(s) of those chemicals to obtain additional MSDSs. 3-2 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_03_ChemSafety.fm

27 Hazardous Chemicals Hazardous Chemicals Overview This instrument may use chemicals that are hazardous. CHEMICAL HAZARD. Hazardous chemicals used with this instrument can cause injury, illness, or death. Handle all chemicals as potentially hazardous. Chemicals are classified as hazardous when they are physically hazardous or if they can cause acute or chronic health hazards upon exposure. Physically hazardous chemicals are materials that are flammable, combustible, under compression (gases), explosive, oxidative, organically peroxidic, pyrophoric, reactive or unstable, or water reactive. Chemicals that may cause health hazards include carcinogens; materials that are toxic or highly toxic; reproductive toxins; irritants; corrosives; sensitizers; materials that are toxic to the liver, kidney or blood-forming (hematopoietic) system; and agents that damage the lungs, skin, eyes, or mucous membranes. Handling Hazardous Chemicals Here are some of the important requirements for handling hazardous chemicals: Read and understand all applicable MSDSs before handling hazardous chemicals. Always wear gloves, safety glasses, and protective clothing when handling chemicals. Always provide adequate ventilation when handling chemicals. Some chemicals require handling only in a properly functioning fume hood. Provide secondary containment for all reagent bottles. Do not store chemicals in direct sunlight or heat (on or off the instrument). BOTTLE FRACTURING HAZARD. When replacing reagents, always install a new bottle on the instrument. Do not add new solution to previously used reagent bottles. Some chemicals reduce the integrity of glass bottles. As a result, repeated use beyond 6 weeks may result in the bottle fracturing when it is pressurized during operation. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 3-3 April 9, :25 pm, SPG_03_ChemSafety.fm

28 Chapter 3 Chemical Safety Hazardous Waste Overview This instrument may generate hazardous waste. CHEMICAL WASTE HAZARD. Wastes produced by Applied Biosystems instruments are potentially hazardous, and can cause injury, illness, or death. Read and understand the material safety data sheets (MSDSs) provided by the manufacturers of the chemicals in the waste container before you store, handle, or dispose of chemical waste. Venting hazardous waste may require local, state/provincial, or national air permits. Minimize contact with chemicals. Wear appropriate personal protective equipment when handling chemicals (for example, safety glasses, gloves, or protective clothing). For additional safety guidelines, consult the MSDS. Minimize the inhalation of chemicals. Do not leave chemical containers open. Use only with adequate ventilation (for example, fume hood). For additional safety guidelines, consult the MSDS. Handle chemical wastes in a fume hood. Seal the waste container with the cap provided. Dispose of the contents of the waste tray and waste bottle in accordance with good laboratory practices and local, state/provincial, or national environmental and health regulations. Instrument Waste System Handling Chemical Waste The waste for this instrument is collected in two containers: A 10-L (2.5-gal) polyethylene container for non-chlorinated waste A 6-L (1.5-gal) polyethylene container for chlorinated waste Dispose of the waste when the waste containers are 3/4 full. The composition of the chemical waste may vary depending upon the protocols used, the number of samples, the volumes specified, and the reagents included in the protocols. When handling chemical waste Applied Biosystems strongly recommends that you: Read the waste profile(s) in this chapter before handling or disposing of hazardous waste. Read all applicable MSDSs before handling or disposing of hazardous waste. Ensure that the waste container is correctly installed. Always handle hazardous materials beneath a fume hood that is connected in accordance with all installation requirements. Always wear chemical-resistant gloves, safety glasses, and protective clothing when handling hazardous waste material. During transfer, ensure that the waste container is tightly sealed with the waste cap provided. Dispose of hazardous waste in accordance with all local, state/provincial, or national regulations. 3-4 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_03_ChemSafety.fm

29 Waste Profiles Storing Hazardous Waste Disposing of Hazardous Waste CHEMICAL STORAGE HAZARD. Never collect or store waste in a glass container because of the risk of breaking or shattering. Reagent and waste bottles can crack and leak. Each waste bottle should be secured in a lowdensity polyethylene safety container with the cover fastened and the handles locked in the upright position. Wear appropriate eyewear, clothing, and gloves when handling reagent and waste bottles. The following are guidelines for storing hazardous waste: Always use secondary containment when storing chemical waste. Store waste for only short periods of time. Store only small amounts of waste in the laboratory. Store waste away from direct sunlight or sources of heat (on or off the instrument). As the generator of potentially hazardous waste, it is your responsibility to: Characterize the waste generated with your applications. Ensure the health and safety of all personnel in your laboratory. Ensure that instrument waste is stored, transferred, transported, and disposed of according to all local, state/provincial, or national regulations. Waste Profiles Overview About the Waste Profiles The waste profiles for installation and for the instrument are provided to assist you in disposing of hazardous waste in accordance with local, state/provincial, or national regulations. The waste profiles include the following: Percent composition of chemicals in the instrument waste Chemical Abstract Service (CAS) number, if the chemical is not a mixture. Installation Waste Profile The installation waste profile characterizes the waste produced during the installation process performed by the Applied Biosystems representative. Instrument Waste Profile The instrument waste profile characterizes the waste produced during a typical user application. The specific application profiled was chosen to provide the most useful information to the largest number of users, given the variety of uses of the instrument. It may not be an application used in your laboratory. The composition of the chemical waste may vary depending upon the specific reagents, the number of samples, and the volumes specified in each application. IMPORTANT! The instrument waste profile is not a substitute for MSDS information. See specific MSDSs for chemical constituent, health, toxicological, and hazard information. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 3-5 April 9, :25 pm, SPG_03_ChemSafety.fm

30 Chapter 3 Chemical Safety Installation Waste Profile The following table characterizes the waste that will be in the waste container of the instrument at the end of the installation process. Installation Waste Profile CAS # Chemical Weight Percent Composition Acetonitrile 100 Instrument Waste Profile The following table characterizes the waste that will be in the waste container after a typical run. Instrument Waste Profile CAS # Chemical Weight Percent Composition Chlorinated Waste Container Trichloroacetic acid Dichloromethane Acetonitrile 5.7 Non-Chlorinated Waste Container Acetonitrile Tetrahydrofuran Pyridine Methylimidazole Water Acetic anhydride Tetrazole trace Iodine trace 3-6 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_03_ChemSafety.fm

31 Instrument Safety 4 4 Overview In This Chapter Safe Operation Routine Maintenance for Safe Operation This chapter provides you with the safety information you need to prepare your laboratory and personnel for the installation and use of the Applied Biosystems 3400 DNA Synthesizer (PN ). The safety labels and safety alert symbols that may be found on this instrument are provided in several languages. The inputs and outputs for the instrument are also provided. This guide provides only site preparation information. Before operating this instrument, read the information in the Applied Biosystems 3400 DNA Synthesizer User Guide (PN ) concerning hazards and potential hazards. Ensure that anyone involved with the operation of the instrument is instructed both in general safety practices for laboratories and in specific safety practices for the instrument. Detailed safety information on the following topics is included in the user guide for this instrument: Compressed gas safety Electrical safety Pressurized bottle safety Maintain the instrument in good working order. If the instrument has been subjected to adverse environmental conditions (such as fire, flood, or earthquake), an Applied Biosystems service representative should inspect the instrument. Applied Biosystems recommends that an Applied Biosystems service representative check the instrument yearly to verify that: The safety interlocks protecting the user from various hazards are working properly. The protective housing is functional. Loose or distorted panels will not protect the user or the equipment. Airflow is not hindered in any way. Instrument Labeling Instrument Safety Labels Safety labels are located on the instrument. Each safety label has three parts: A signal word panel, which implies a particular level of observation or action (for example, CAUTION or WARNING). If a safety label encompasses multiple hazards, the signal word corresponding to the greatest hazard is used. A message panel, which explains the hazard and any user action required. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 4-1 April 9, :25 pm, SPG_04_InstSafety.fm

32 Chapter 4 Instrument Safety A safety alert symbol, which indicates a potential personal safety hazard. See Safety Alert Symbols on page 4-4 for an explanation of all the safety alert symbols. This information is provided in several languages. Signal Words CAUTION indicates a potentially hazardous situation that could result in minor or moderate injury to the user or damage to the instrument. WARNING indicates a potentially hazardous situation that could result in death or serious injury. DANGER indicates an imminently hazardous situation that, if not avoided, will result in death or serious injury (most extreme). Parts of an Instrument Safety Label Parts of an instrument safety label are identified below. DANGER Signal word LASER RADIATION WHEN OPEN AND INTERLOCK DEFEATED AVOID DIRECT EXPOSURE TO BEAM Message panel Safety alert symbol Examples of Instrument Safety Labels Below are some examples of instrument safety labels. DANGER HIGH VOLTAGE CAUTION - HOT CAUTION - MOVING PARTS Labels That May Be Found on the Instrument The following Danger, Caution, and Warning labels, listed in English and French, may be found on your instrument: English CAUTION: Hazardous chemicals. Read the Material Safety Data Sheets (MSDSs) before handling. CAUTION: Hazardous waste. Read the waste profile before handling or disposal. WARNING: Risk of electric shock. Disconnect power cord from supply before replacing fuses or removing power supply module from instrument. WARNING: For continued protection against risk of fire, replace only with Listed and Certified fuse of the specified type and ratings. WARNING: Hot lamp. French ATTENTION: Produits chimiques dangeureux. Lire les fiches techniques de sûreté de matériels avant la manipulation des produits. ATTENTION: Déchets dangereux. Lire les renseignements sur les déchets avant de les manipuler ou de les éliminer. AVERTISSEMENT: Risque de choc électrique. Débrancher le cordon d alimentation avant de remplacer les fusibles ou de retirer le bloc d alimentation de l instrument. AVERTISSEMENT: Pour une protection continue contre les risques d incendie, utiliser uniquement des fusibles agréés et certifiés du type et du courant nominal spécifiés. AVERTISSEMENT: Lampe brûlante. 4-2 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_04_InstSafety.fm

33 Instrument Labeling English WARNING: Hot. Replace lamp with an Applied Biosystems lamp. WARNING: Disconnect supply cord before opening. Grounding circuit continuity is vital for safe operation of equipment. Never operate equipment with grounding conductor disconnected. WARNING: For protection against fire hazard, replace only same type and rating of fuse. CAUTION: Hot. DANGER: High voltage. WARNING: To reduce the chance of electrical shock, do not remove covers that require tool access. No user serviceable parts are inside. Refer servicing to Applied Biosystems qualified service personnel. DANGER: Laser radiation when open and interlock defeated. Avoid direct exposure to beam. CAUTION: Moving parts. French AVERTISSEMENT: Composants brûlants. Remplacer la lampe par une lampe Applied Biosystems. AVERTISSEMENT: Débrancher le cordon d alimentation avant d ouvrir. La continuité du circuit de masse est essentiel à la sécurité du fonctionnement de l appareil. Ne jamais utiliser l appareil avec la prise de terre débranchée. AVERTISSEMENT: Pour assurer la protection contre les risques d incendie, remplacer uniquement par un fusible de même type et de même courant nominal. ATTENTION: Surface brûlante. DANGER: Haute tension. AVERTISSEMENT: Pour éviter les risques d'électrocution, ne pas retirer les capots dont l'ouverture nécessite l'utilisation d'outils. L instrument ne contient aucune pièce réparable par l utilisateur. Toute intervention doit être effectuée par le personnel de service qualifié de Applied Biosystems. DANGER: Rayonnement laser en cas d ouverture et d une neutralisation des dispositifs de sécurité. Eviter toute exposition directe avec le faisceau. ATTENTION: Parties mobiles. Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide 4-3 April 9, :25 pm, SPG_04_InstSafety.fm

34 Chapter 4 Instrument Safety Safety Alert Symbols Electrical Symbols The following chart is an illustrated glossary of all electrical symbols that are used on Applied Biosystems instruments. Whenever such symbols appear on instruments, please observe appropriate safety procedures. This symbol indicates the On position of the main power switch. This symbol indicates the Off position of the main power switch. This symbol indicates the On/Off position of a push-push main power switch. This symbol indicates that a terminal may be connected to another instrument s signal ground reference. This is not a protected ground terminal. This symbol indicates that this is a protective grounding terminal that must be connected to earth ground before any other electrical connections are made to the instrument. A terminal marked with this symbol either receives or delivers alternating current or voltage. A terminal marked with this symbol can receive or supply an alternating and a direct current or voltage. This symbol indicates the presence of high voltage and warns the user to proceed with caution. This symbol alerts you to consult the manual for further information and to proceed with caution. Non-Electrical Symbols The following is an illustrated glossary of all nonelectrical safety alert symbols found on Applied Biosystems instruments. This symbol illustrates a heater hazard. Proceed with caution when working around these areas to avoid being burned by hot components. This symbol indicates that a laser is present inside the instrument. 4-4 Applied Biosystems 3400 DNA Synthesizer Site Preparation and Safety Guide April 9, :25 pm, SPG_04_InstSafety.fm