CHE-554 Biological Chemistry Lab. SAFETY in the Biological Chemistry Lab. Prof. A.-F. Miller 21 January 2014

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1 CHE-554 iological Chemistry Lab! Tues and Thurs, 12:20 pm CP 222 & 1:30 or 3:30 in CP 236! ring lab coat, safety glasses, US memory stick, notebook.! Wear sensible clothing: closed-toe shoes, no flamable synthetics or loose items.! e prepared in advance for each day.! COME! Watch for these techniques in the literature, and the news.! Why did you choose this course?! Syllabus, and more, on the web site www/chem.uky.edu/courses/che554 1 SFETY in the iological Chemistry Lab Prof..-F. Miller 21 January 2014 lso: if you have a thumbdrive with you today, see me after class. 2.-F. Miller F. Miller 2014

2 SFETY in the iological Chemistry Lab 3 General Laboratory Safety 4! Your safety equipment: ttitude lert Prepared Skill and experience Correct Equipment Equipment is in good condition Good environment (lighting, distractions).! When do accidents happen? Tired, bored, stressed, distracted Don t have the correct tools Other?! The biological chemistry laboratory can be a dangerous place if:! You are not being careful and not paying attention to what you are doing.! You do not understand what you are doing.! You do not use equipment or techniques properly. Don t work alone unless the task is genuinely routine for you..-f. Miller F. Miller 2014

3 uthorized ctivities and Proper Conduct! It is very important that: 5 Most common reasons experiments fail 6! You follow the operating procedures suggested in the handouts, the textbook, and in equipment manuals.! You only perform the assigned experiments.! You pay attention to the direction and warnings of your supervisor or instructor.! You respect the safety of others in every laboratory operation that you perform.! Establish a buddy system, and use it.! Errors following the protocol Write and diagram out an action plan for the entire experiment, compiling a list of required reagents and equipment, IN DVNCE. Check steps off as they are completed, and WRITE DOWN observations regarding the condition of the samples at each step (write down the time as well). Keep an eye on total volumes, these will reveal when a reagent has not been added, or been added in the wrong quantity. Check all solutions, before using them (eg. confirm ph)..-f. Miller F. Miller 2014 This will be done for you.

4 .-F. Miller 2014 Dressed to live 7.-F. Miller 2014 Dress Code! Safety glasses must be worn at all times. It is recommended that you not wear contact lenses in the laboratory.! Minimize skin exposure. Legs and feet should be completely covered. Long pants, skirts, dresses, etc. must cover legs and ankles. Shorts, capri pants, tank tops, midriff shirts, muscle shirts, open-toed shoes, and sandals are not permitted.! Wear a lab coat at all times.! Wear exam gloves at all times.! Tie back or confine long hair and loose items of clothing and jewelry.! Choose non-flammable clothing.! Turn off any distractions: cell phones etc. NEVER wear head phones in a lab.! Do not even enter the room if you are under the influence of any disruptive substance: alcohol, sedatives, drugs, etc. 8

5 Flammable Materials 9 iohazards 10! Keep flames away from the area where you use ether, acetone, methanol, ethanol or other organic solvents.! single plasmid molecule can confer drug resistance on your resident bacterial flora.! Your resident bacterial flora can eat your experiment.! Handle flammable solvents in a fume hood.! Do not inhale chemical vapours. They can be deadly.! Wear non-synthetic clothing and shoes that provide a barrier to liquid penetration (athletic shoes w. mesh top and sides fail this test).! Dyes such as ethidium bromide are carcinogens with delayed but serious toxicity.! Gloved hands are research hands. Research hands do research, ONLY: no hair adjustments, wiping face, use of public facilities... (avoid using your keyboard or cell phone)..-f. Miller F. Miller 2014

6 Spills! ll spills must be cleaned immediately (biological or chemical).! iologicals or chemicals spilled on the skin should be washed off immediately by flushing with water for 15 minutes. Notify T.. immediately.! In case of a major accident, seek medical treatment immediately. (lso notify T.. and instructor.) 11 Waste Disposal! Dispose of all waste properly. Waste containers will be provided. If unsure, ask your supervisor or instructor.! Fully read the labels on chemical and waste containers before disposal.! Do not forget to close the container lid when done. For volatile substances, do not tighten the lid.! iohazard materials must be disposed off in appropriate bags. Infectious Material must be disposed of in red bags. 12! Sharp materials such as gel cutters, razor blades, and syringe needles much be disposed off in sharps container..-f. Miller F. Miller 2014

7 .-F. Miller 2014 Concentrated cids and ases When handling acids or bases:! e careful to avoid splashing them on skin or clothes.! Dilute by adding the reagent to water (never add water to the reagent). 13.-F. Miller 2014 Toxic Chemicals! Toxic chemicals such as acrylamide, caustic solutions, cyanides, oxalates, mercury salts, are hazardous to humans.! Never handle such chemicals such that your skin is in direct contact with them.! Never mouth-pipette anything.! Use impermeable gloves and nose mask while weighing or using these chemicals.! Wash any chemical spills off your body promptly.! Wash your hands with soap after EVERY session in lab, regardless of whether any mishap occurred.! Familiarize yourself will all reagents beforehand. 14

8 Safety of reagents 15 Safety of reagents 16! MSDS! Merck Manual! Web sites! chemicals/aspirin.html provides a simplified version. uthentic ones for aspirin and glucose are available via the course web site.! lso go through MSDS often prepared with the intension of providing legal protection to the manufacturer, rather than practical information for the user. Look at the LD50, IC50 Know these values as they apply to a human (you!)! For dangerous materials Know exposure routes Know protective devices and protocols Know antidotes and first-response actions.! YOU are your brother s keeper, and your sister s too..-f. Miller F. Miller 2014

9 Safety Equipment 17 Handling Glass 18! Know the location of all safety equipment Showers Eye washes fire extinguishers First aid kit Evacuation plan! Glass cuts are among the most common lab accidents. Even the most experienced chemists are susceptible. Please be careful.! Do not insert glass tubing into rubber stoppers or corks.! Discard cracked and chipped glassware in glass disposal box..-f. Miller F. Miller 2014

10 Reporting ccidents! Report an accident or an injury, no matter how minor, to the instructor or laboratory supervisor immediately. n accident report form must be completed.! first aid kit for minor cuts or burns is available in the laboratory. sk the instructor or laboratory supervisor for help..-f. Miller Safety Information! In Case of an Emergency dminister First id Stay with the injured person! Call 9-911! Important Phone Numbers: UK Police Dept. (7-8573) Environmental Health and Safety (7-3241) Occupational Health and Safety (7-3827) Hazardous Materials Management (3-6280)! ccident Report Form Environmental/ohs/! dhere to uilding Emergency Evacuation Plan. Take note of your locker-mate s successful evacuation. Stay together as a class to facilitate accounting for all members..-f. Miller

11 Section1 Lockers & Groups 21 Name Locker Group Danielle Edwards L41C L41C n Lien Ho L35C L35C L44C Niranjana Warrier L44C L34C Morgan Sizemore L34C Corey Lee L31C Jared Phillips L31C Derrick Lewis L28C L28C Ryan Melody L25C L25C Travis Johnson L38C Jessime Kirk L38C L44C Matthew Wolfe L44C Kaeto Vin-Nnajiofor L41C L41C L35C Joanna Ng L35C.-F. Miller 2014 Section2 Lockers & Groups 22 Name Locker Group L41C Robert Reed L41C L35C Falak Patel L35C Carl rchemetre L44C L44C Emily ryant L34C L34C L31C L31C L28C L28C.-F. Miller 2014 L25C Hagen Smith L25C L38C L38C Kalen Wright L44C L44C L41C L41C Dylan Woolum L35C L35C

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