Plants in a Bottle: Photosynthesis and Respiration Teacher Information

Transcription

1 STO-144 Plants in a Bottle: Photosynthesis and Respiration Teacher Information Summary Kit contains Students conduct simulated tests to compare the amounts of oxygen produced and used by aquatic plants under various environmental conditions. Core Concept Plants carry out photosynthesis to store energy in food molecules. Photosynthesis uses carbon dioxide and water and produces food and oxygen. Cellular respiration releases the energy stored in food molecules. 4 simulated water samples from bottles containing plants exposed to different light levels Oxygen Indicator (simulated) Carbon Dioxide Indicator (simulated) 6 labeled droppers Oxygen and Carbon Dioxide Test Sheet Oxygen and Carbon Dioxide Concentration Color Charts Photosynthesis and Respiration Diagram Sheet Plants carry out cellular respiration to produce ATP energy needed for life activities and growth. Cellular respiration uses food and oxygen and produces carbon dioxide and water as wastes. Teacher Provides Optional: Copy of Photosynthesis and Respiration Diagram Sheet for each student if Part 1 is assigned for homework. This may be printed in black and white. Time Required 1-2 forty-minute class period(s) Safety goggles Paper towels for cleanup Warning: Choking Hazard This Science Take-Out kit contains small parts. Do not allow children under the age of seven to have access to any kit components. Copyright 2015 by Science Take-Out, Take-Out. LLC. All rights All rights reserved. reserved. This document may be copied for use only with Science Take-Out educational materials. This document may not be reproduced or distributed for any other purpose without written consent from Science Take-Out. i

2 Reusing Plants in a Bottle kits Kits may be refilled and reused. Allow approximately minutes for refilling 10 student kits. Teachers will need to instruct students on how to handle clean-up and return of the reusable kit materials. For example, teachers might provide the following information for students: Rinse with water and dry with paper towel Plastic test sheet Droppers Refill and return to kit Tubes of water samples and indicators All droppers Plastic test sheet Color chart Note: Do not immerse droppers in water. To rinse, simply squirt the contents out of the dropper and then draw water up into the dropper several times. Empty water out of dropper. Dry outside of dropper. Refills for kits are available at The 10 Kit Refill Pack includes the following materials: Instructions and Quick Guide for refilling kits 10 ml of simulated water samples for Bottle 1, Bottle 2, Bottle 3, and Bottle 4 10 ml of simulated Oxygen Indicator 10 ml of simulated Carbon Dioxide Indicator 6 graduated transfer pipets (for refilling the microtubes) Extension Activity Challenge students to design and create their closed ecosystem with plants sealed in a bottle. To avoid potential breakage, it is strongly recommended that students use clear plastic, not glass, containers. Copyright 2015 by Science Take-Out. All rights reserved. ii

3 Kit Contents Quick Guide Copyright 2015 by Science Take-Out. All rights reserved. iii

4 Read these instructions before using Science Take-Out kits Parental or Adult Supervision Required This kit should be used only under the supervision of an adult who is committed to ensuring that the safety precautions below, and in the specific laboratory activity, are followed. Safety Goggles and Gloves Strongly Recommended We encourage students to adopt safe lab practices, and wear safety goggles and gloves when performing laboratory activities involving chemicals. Safety goggles and gloves are not provided in Science Take-Out kits. They may be purchased from a local hardware store or pharmacy. Warning: Choking and Chemical Hazard Science Take-Out kits contain small parts that could pose a choking hazard and chemicals that could be hazardous if ingested. Do not allow children under the age of seven to have access to any kit components. Material Safety Data Sheets (MSDS) provide specific safety information regarding the chemical contents of the kits. MSDS information for each kit is provided in the accompanying teacher instructions. Chemicals Used in Science Take-Out Kits Every effort has been made to reduce the use of hazardous chemicals in Science Take-Out kits. Most kits contain common household chemicals or chemicals that pose little or no risk. General Safety Precautions 1. Work in a clean, uncluttered area. Cover the work area to protect the work surface. 2. Read and follow all instructions carefully. 3. Pay particular attention to following the specific safety precautions included in the kit activity instructions. 4. Goggles and gloves should be worn while performing experiments using chemicals. 5. Do not use the contents of this kit for any other purpose beyond those described in the kit instructions. 6. Do not leave experiment parts or kits where they could be used inappropriately by others. 7. Never taste or ingest any chemicals provided in the kit - they may be toxic. 8. Do not eat, drink, or apply make-up or contact lenses while performing experiments. 9. Wash your hands before and after performing experiments. 10. Chemicals used in Science Take-Out experiments may stain or damage skin, clothing or work surfaces. If spills occur, wash the area immediately and thoroughly. 11. At the end of the experiment, return ALL kit components to the kit plastic bag. Dispose of the plastic bag and contents in your regular household trash. No blood or body fluids from humans or animals are used in Science Take-Out kits. Chemical mixtures are substituted as simulations of these substances. Copyright 2015 by Science Take-Out. All rights reserved. iv

5 Plants in a Bottle: Photosynthesis and Respiration Teacher Answer Key My grandfather has a large bottle filled with water, soil, and plants. For the last 50 years, the plants have been completely sealed from the outside world. It is really low maintenance! My grandfather does not need to add more water to the sealed bottle. He keeps the bottle about six feet from a sunny window. The plants grow toward the light, so he turns the bottle around occasionally so that the plants grow evenly. Part 1: Photosynthesis and Cellular Respiration 1. Use the Photosynthesis and Respiration Diagram Sheet in the kit. For each of the following statements, circle the word on the right to indicate whether the statement is true for photosynthesis, respiration, or both. Uses food (sugar) Photosynthesis Respiration Both Makes food (sugar) Photosynthesis Respiration Both Uses oxygen Photosynthesis Respiration Both Makes oxygen Photosynthesis Respiration Both Uses carbon dioxide Photosynthesis Respiration Both Makes carbon dioxide Photosynthesis Respiration Both Occurs during the day Photosynthesis Respiration Both Occurs at night Photosynthesis Respiration Both Makes ATP for life activities Photosynthesis Respiration Both Occurs in plants Photosynthesis Respiration Both Occurs in mitochondria Photosynthesis Respiration Both Occurs in chloroplasts Photosynthesis Respiration Both Copyright 2015 by Science Take-Out. All rights reserved. 1

6 2. For the plants to survive, what must enter the bottle? Light 3. In your own words, explain why a plant carries out the process of photosynthesis. Plants carry out photosynthesis to make the food they need for growth and cellular respiration. 4. In your own words, explain why a plant carries out the process of cellular respiration. Plants carry out cellular respiration to convert the energy stored in food into the energy for life activities. Copyright 2015 by Science Take-Out. All rights reserved. 2

7 Part 2: Photosynthesis and Respiration Affect the Oxygen Concentration In Part 2, you will collect and analyze data from an experiment to investigate how photosynthesis and cellular respiration affect the oxygen concentration. A student set up 4 clear, sealed bottles. Each bottle was filled with 5 grams of aquatic plants and 500 ml of water. The student determined that the initial (beginning) concentration of oxygen in each of the bottles was 6 parts per million (ppm). The bottles were then placed in environments that had the same temperatures but different light intensities. After 24 hours, water samples were removed from each of the 4 bottles. You will measure the concentration of oxygen in each of the bottles after 24 hours. 1. The independent variable in an experiment is the variable that is changed by the scientist. To ensure a fair test, a good experiment has only one independent variable. What is the independent variable in the experiment described in the box above? Light Intensity or the amount of light or the type of light 2. As the scientist changes the independent variable, he or she observes or measures the dependent variable. This will measure how the dependent variable responds when change is made to the independent variable. What is the dependent variable in the experiment described in the box above? Concentration of oxygen in the water Copyright 2015 by Science Take-Out. All rights reserved. 3

8 3. A well-designed experiment also has controlled or constant variables. These are things, other than the independent variable, that could affect the results of an experiment. An experiment would not be a fair test unless these controlled variables were kept the same in all four bottles. List at least three controlled variables that were kept constant to ensure that there was only one variable (the independent variable) in the experiment. Amount of plant, initial oxygen concentration, temperature, amount of water, and size of bottle 4. Photosynthesis converts light energy into energy stored in food. In which bottles do you think that photosynthesis is occurring? Explain in detail why you think photosynthesis is occurring in these bottles. Student answers may vary but likely answers include Bottles 1, 2, and 3 because they have light. 5. Respiration converts energy stored in food into ATP energy used for carrying out life activities. In which bottles do you think respiration is occurring? Explain in detail why you think respiration is occurring in these bottles. Student answers may vary. Respiration is occurring in all four bottles. Many students answer only Bottle 4. Copyright 2015 by Science Take-Out. All rights reserved. 4

9 6. Use the tube of Oxygen Indicator and the instructions below to determine the FINAL oxygen concentration in each of the water samples (Bottles 1-4). Be careful to use the droppers with labels that match the labels on the tubes. Place 1 drop of Oxygen Indicator in each of the circles in the Oxygen Test Column on the plastic Test Sheet. Place 2 drops of water samples from each bottle in the appropriate circles in the left column of the Test Sheet. Wait for 15 seconds and then compare the colors of the liquid in each circle of the test sheet with the Oxygen Concentration Color Chart. ppm parts per million A measure of the number of oxygen molecules in 1 million molecules of air Record the FINAL oxygen concentration (ppm) for each sample in the data table below. Be sure to include a + or sign in your answer! Bottle 1 Bright Light 2 Medium Light 3 Low Light 4 Dark FINAL Oxygen Concentration (ppm) INITIAL Oxygen Concentration (ppm) NET CHANGE in Oxygen Concentration (ppm) Final Initial = Net Change Complete the NET CHANGE in Oxygen Concentration column in the data table. Be sure to include the + or sign in your answer. FINAL oxygen concentration INITIAL oxygen concentration = NET CHANGE in oxygen concentration A + sign in your answer indicates that more oxygen is produced than used. A sign in your answer indicates that more oxygen is used than made. A 0 for your answer indicates that the oxygen used equals the oxygen produced. Copyright 2015 by Science Take-Out. All rights reserved. 5

10 All plants need to carry out respiration to change stored food energy into ATP energy needed for their life activities. Respiration is occurring in ALL four bottles. The amount of oxygen used for respiration in Bottle 4 is the same as the amount of oxygen used for respiration in the other three bottles (1, 2, and 3) Represent the amount of oxygen used for respiration in all four bottles by writing R = 4 in each of the bottles above (1, 2, 3, and 4) to represent the amount of oxygen used for respiration (4 ppm). 9. Use the information from the last column on your data table to write the NET CHANGE in oxygen after the N = for each bottle above. Note: See your data table on the previous page. 10. Use the equation shown below to calculate P (the oxygen produced by photosynthesis in the plants). Record your answer after the P = on the diagrams of bottles above. N = P R Net change Oxygen produced Oxygen used in oxygen by photosynthesis for respiration Show your work for each of the bottles. Bottle 1 Bottle 2 Bottle 3 Bottle = P 4 P = = P 4 P = 6 0 = P 4 P = 4-4 = P 4 P = 0 Copyright 2015 by Science Take-Out. All rights reserved. 6

11 11. In which bottle or bottles is the amount of oxygen produced by photosynthesis greater than the amount of oxygen used for respiration? Support your answer with information from the diagrams on the previous page. Bottle 1 and bottle 2 because the change is positive (the net amount of oxygen increased). The amount of oxygen produced by photosynthesis is greater than the amount of oxygen used for cellular respiration. 12. In which bottle or bottles is the amount of oxygen produced by photosynthesis equal to the amount of oxygen used in cellular respiration? Support your answer with information from the diagrams on the previous page. Bottle 3 because there is no change or because the net amount of oxygen remained the same. This means that the amount of oxygen produced by photosynthesis is equal to the amount of oxygen used for respiration. 13. In which bottle or bottles is the amount of oxygen produced by photosynthesis less than the amount of oxygen used in cellular respiration? Support your answer with information from the diagrams on the previous page. Bottle 4 because the change in oxygen is negative or because the net amount of oxygen decreased. No oxygen can be produced by photosynthesis because there is no light. 14. In which bottle would you expect the most plant growth? Explain why you chose your answer in detail. Bottle 1 because it is the bottle that has the greatest light intensity resulting in the greatest amount of photosynthesis which would make the greatest amount of food for growth. Copyright 2015 by Science Take-Out. All rights reserved. 7

12 15. PREDICT - In which bottle would you expect to find the lowest concentration of carbon dioxide? Explain why you chose your answer. Bottle 1 because it is the bottle that has the greatest amount of photosynthesis. Photosynthesis uses carbon dioxide. 16. Test your prediction. Use the tube of Carbon Dioxide Indicator and the instructions below to determine the carbon dioxide concentration in each of the water samples (Bottles 1-4). Place 1 drop of Carbon Dioxide Indicator in each of the circles in the Carbon Dioxide Test Column on the plastic Test Sheet. Place 2 drops of water samples from each bottle in the appropriate circles as indicated in left column. Wait for 10 seconds and then compare the colors of the liquid in each circle with the Carbon Dioxide Concentration Color Chart. According to the test results, which bottle had the lowest concentration of carbon dioxide? 17. Was your prediction in question 15 correct? If not, how would you modify your prediction and explanation to be supported by the carbon dioxide testing results? Students may say yes or no. If they say no, they should explain that the concentration of carbon dioxide would be greatest in the bottle that is carrying out the least photosynthesis. 18. Scientists believe that the concentration of carbon dioxide in the atmosphere is increasing. Describe two human activities which may lead to an increase in carbon dioxide in the atmosphere. Burning fossil fuels and destroying plant life. Copyright 2015 by Science Take-Out. All rights reserved. 8

13 SAFETY DATA SHEET GENERAL STORAGE CODE GREEN Section 1 Chemical Product and Company Information Science Take-Out 80 Office Park Way Pittsford, NY CHEMTREC 24 Hour Emergency Phone Number (800) (585) For laboratory use only. Not for drug, food or household use. Product Buffer Solution ph10 Synonyms Bottle 1 Water Sample (simulated) Section 2 Hazards Identification This substance or mixture has not been classified at this time according to the Globally Harmonized System (GHS) of Classification and Labeling of Chemicals. Signal word: WARNING Pictograms: None required Target organs: None known GHS Classification: Skin irritation (Category 3) Eye irritation (Category 2B) Precautionary statement(s): P264: Wash hands thoroughly after handling. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P332+P313: If skin irritation occurs: Get medical attention. P337+P313: If eye irritation persists: Get medical attention. GHS Label information: Hazard statement(s): H316: Causes mild skin irritation. H320: Causes eye irritation. Ca Prop 65 - This product does not contain any chemicals known to the State of California to cause cancer, birth defects, or any other reproductive harm. Section 3 Composition / Information on Ingredients Chemical Name CAS # % EINECS Water % Potassium chloride % Boric acid % Sodium hydroxide % Section 4 First Aid Measures INGESTION: Call physician or Poison Control Center immediately. Induce vomiting only if advised by appropriate medical personnel. Never give anything by mouth to an unconscious person. INHALATION: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention. EYE CONTACT: Check for and remove contact lenses. Flush thoroughly with water for at least 15 minutes, lifting upper and lower eyelids occasionally. Get immediate medical attention. SKIN ABSORPTION: Remove contaminated clothing. Flush thoroughly with mild soap and water. If irritation occurs, get medical attention. Section 5 Fire Fighting Measures Suitable Extinguishing Media: Use any media suitable for extinguishing supporting fire. Protective Actions for Fire-fighters: In fire conditions, wear a NIOSH/MSHA-approved self-contained breathing apparatus and full protective gear. Use water spray to keep fire-exposed containers cool. Specific Hazards: During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Section 6 Accidental Release Measures Personal Precautions: Evacuate personnel to safe area. Use proper personal protective equipment as indicated in Section 8. Provide adequate ventilation. Environmental Precautions: Avoid runoff into storm sewers and ditches which lead to waterways. Containment and Cleanup: Absorb with inert dry material, sweep or vacuum up and place in a suitable container for proper disposal. Wash spill area with soap and water. Section 7 Handling and Storage Precautions for Safe Handling: Read label on container before using. Do not wear contact lenses when working with chemicals. Keep out of reach of children. Avoid contact with eyes, skin and clothing. Do not inhale vapors, spray or mist. Use with adequate ventilation. Avoid ingestion. Wash thoroughly after handling. Remove and wash clothing before reuse. Conditions for Safe Storage: Store in a cool, well-ventilated area away from incompatible substances.

14 Section 8 Exposure controls / Personal Protection Exposure Limits: Chemical Name ACGIH (TLV) OSHA (PEL) NIOSH (REL) Potassium chloride None established None established None established Engineering controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower and fire extinguishing material. Personnel should wear safety glasses, goggles, or faceshield, lab coat or apron, appropriate protective gloves. Use adequate ventilation to keep airborne concentrations low. Respiratory protection: None should be needed in normal laboratory handling at room temperatures. If misty conditions prevail, work in fume hood or wear a NIOSH/MSHA approved respirator. Section 9 Physical and Chemical Properties Appearance: Clear, colorless liquid. Evaporation rate ( Water = 1): <1 Odor: No odor. Flammability (solid/gas): Data not available. Odor threshold: Data not available. Explosion limits: Lower/Upper: Data not available ph: 10.0 Vapor pressure (mm Hg): 14 (water) Melting/Freezing point: Approx. 0 C (32 F) (water) Vapor density (Air = 1): 0.7 (water) Boiling point: Approx. 100 C (212 F) (water) Relative density (Specific gravity): Approx. 1.0 (water) Flash point: Data not available Solubility(ies): Complete in water. Section 10 Stability and Reactivity Chemical stability: Stable Hazardous polymerization: Will not occur. Conditions to avoid: Excessive temperatures which cause evaporation. Incompatibilities with other materials: Acids, alkalies, and air will change the buffer s ability. Hazardous decomposition products: Boron oxide and chlorine gas. Partition coefficient: Data not available Auto-ignition temp.: Data not available Decomposition temp.: Data not available Viscosity: Data not available. Molecular formula: Mixture Molecular weight: Mixture Section 11 Toxicological Information Acute toxicity: Data not available Skin corrosion/irritation: Data not available Serious eye damage/irritation: Data not available Respiratory or skin sensitization: Data not available Germ cell mutagenicity: Data not available Carcinogenity: Data not available NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP. IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Reproductive toxicity: Data not available STOT-single exposure: Data not available Aspiration hazard: Data not available STOT-repeated exposure: Data not available Potential health effects: Inhalation: May be harmful if inhaled. Ingestion: May be harmful if swallowed. Skin: May cause mild irritation. Eyes: May cause mild irritation. Signs and symptoms of exposure: To the best of our knowledge the chemical, physical and toxicological properties have not been thoroughly investigated. Specific data is not available. Exercise appropriate procedures to minimize potential hazards. Additional information: RTECS #: Data not available Section 12 Ecological Information Toxicity to fish: No data available Toxicity to daphnia and other aquatic invertebrates: No data available Toxicity to algae: No data available Persistence and degradability: No data available Bioaccumulative potential: No data available Mobility in soil: No data available PBT and vpvb assessment: No data available Other adverse effects: An environmental hazard cannot be excluded in the event of unprofessional handling or disposal. Section 13 Disposal Considerations These disposal guidelines are intended for the disposal of catalog-size quantities only. Federal regulations may apply to empty container. State and/or local regulations may be different. Dispose of in accordance with all local, state and federal regulations or contract with a licensed chemical disposal agency. Section 14 Transport Information UN/NA number: Not applicable Shipping name: Not Regulated Hazard class: Not applicable Packing group: Not applicable Reportable Quantity: No Marine pollutant: No Exceptions: Not applicable 2012 ERG Guide # Not applicable Section 15 Regulatory Information A chemical is considered to be listed if the CAS number for the anhydrous form is on the Inventory list. Component TSCA CERLCA (RQ) RCRA code DSL NDSL WHMIS Classification Potassium Chloride Listed Not Listed Not Listed Listed Not Listed Uncontrolled Product Sodium hydroxide Listed 1,000 lbs (454 kg) D002 Listed Not Listed E Section 16 Additional Information The information contained herein is furnished without warranty of any kind. Employers should use this information only as a supplement to other information gathered by them and must make independent determinations of suitability and completeness of information from all sources to assure proper use of these materials and the safety and health of employees. NTP: National Toxicology Program, IARC: International Agency for Research on Cancer, OSHA: Occupational Safety and Health Administration, STOT: Specific Target Organ Toxicity, SE: Single Exposure, RE: Repeated Exposure, ERG: Emergency Response Guidebook. Revision Date: March 27, 2015 Supercedes:

15 SAFETY DATA SHEET GENERAL STORAGE CODE GREEN Section 1 Chemical Product and Company Information Science Take-Out 80 Office Park Way Pittsford, NY CHEMTREC 24 Hour Emergency Phone Number (800) (585) For laboratory use only. Not for drug, food or household use Product Synonyms Section 2 Buffer Solution ph7 Bottle 2 Water Sample (simulated) Hazards Identification This substance or mixture has not been classified at this time according to the Globally Harmonized System (GHS) of Classification and Labeling of Chemicals. Signal word: WARNING Pictograms: None required Target organs: None known GHS Classification: Skin Irritation (Category 3) Eye irritation (Category 2B) Precautionary statement(s): P264: Wash hands thoroughly after handling. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P332+P313: If skin irritation occurs: Get medical attention. P337+P313: If eye irritation persists: Get medical attention. GHS Label information: Hazard statement(s): H316: Causes minor skin irritation. H320: Causes eye irritation. Ca Prop 65 - This product does not contain any chemicals known to the State of California to cause cancer, birth defects, or any other reproductive harm. Section 3 Composition / Information on Ingredients Chemical Name CAS # % EINECS Water % Potassium phosphate, monobasic % Sodium hydroxide % Section 4 First Aid Measures INGESTION: Call physician or Poison Control Center immediately. Induce vomiting only if advised by appropriate medical personnel. Never give anything by mouth to an unconscious person. INHALATION: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention. EYE CONTACT: Check for and remove contact lenses. Flush thoroughly with water for at least 15 minutes, lifting upper and lower eyelids occasionally. Get immediate medical attention. SKIN ABSORPTION: Remove contaminated clothing. Flush thoroughly with mild soap and water. If irritation occurs, get medical attention. Section 5 Fire Fighting Measures Suitable Extinguishing Media: Use any media suitable for extinguishing supporting fire. Protective Actions for Fire-fighters: In fire conditions, wear a NIOSH/MSHA-approved self-contained breathing apparatus and full protective gear. Use water spray to keep fire-exposed containers cool. Specific Hazards: During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Section 6 Accidental Release Measures Personal Precautions: Evacuate personnel to safe area. Use proper personal protective equipment as indicated in Section 8. Provide adequate ventilation. Environmental Precautions: Avoid runoff into storm sewers and ditches which lead to waterways. Containment and Cleanup: Absorb with inert dry material, sweep or vacuum up and place in a suitable container for proper disposal. Wash spill area with soap and water. Section 7 Handling and Storage Precautions for Safe Handling: Read label on container before using. Do not wear contact lenses when working with chemicals. Keep out of reach of children. Avoid contact with eyes, skin and clothing. Do not inhale vapors, spray or mist. Use with adequate ventilation. Avoid ingestion. Wash thoroughly after handling. Remove and wash clothing before reuse. Conditions for Safe Storage: Store in a cool, well-ventilated area away from incompatible substances.

16 Section 8 Exposure controls / Personal Protection Exposure Limits: Chemical Name ACGIH (TLV) OSHA (PEL) NIOSH (REL) Potassium phosphate None established None established None established Engineering controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower and fire extinguishing material. Personnel should wear safety glasses, goggles, or faceshield, lab coat or apron, appropriate protective gloves. Use adequate ventilation to keep airborne concentrations low. Respiratory protection: None should be needed in normal laboratory handling at room temperatures. If misty conditions prevail, work in fume hood or wear a NIOSH/MSHA approved respirator. Section 9 Physical and Chemical Properties Appearance: Clear, colorless liquid. Evaporation rate ( Water = 1): <1 Odor: No odor. Flammability (solid/gas): Data not available. Odor threshold: Data not available. Explosion limits: Lower/Upper: Data not available ph: 7.0 Vapor pressure (mm Hg): 14 (water) Melting/Freezing point: Approx. 0 C (32 F) (water) Vapor density (Air = 1): 0.7 (water) Boiling point: Approx. 100 C (212 F) (water) Relative density (Specific gravity): Approx. 1.0 (water) Flash point: Data not available Solubility(ies): Complete in water. Section 10 Stability and Reactivity Chemical stability: Stable Hazardous polymerization: Will not occur. Conditions to avoid: Excessive temperatures which cause evaporation. Incompatibilities with other materials: Acids, alkalies, and air will change the buffer s ability. Hazardous decomposition products: Thermal decomposition will yield phosphates and sodium oxide and/or hydroxides. Partition coefficient: Data not available Auto-ignition temp.: Data not available Decomposition temp.: Data not available Viscosity: Data not available. Molecular formula: Mixture Molecular weight: Mixture Section 11 Toxicological Information Acute toxicity: Oral-rat LD50: 3,200 mg/kg [Potassium phosphate] Skin corrosion/irritation: Data not available Serious eye damage/irritation: Data not available Respiratory or skin sensitization: Data not available Germ cell mutagenicity: Data not available Carcinogenity: Data not available NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP. IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Reproductive toxicity: Data not available STOT-single exposure: Data not available Aspiration hazard: Data not available STOT-repeated exposure: Data not available Potential health effects: Inhalation: May be harmful if inhaled. Ingestion: May be harmful if swallowed. Skin: May cause mild irritation. Eyes: May cause mild irritation. Signs and symptoms of exposure: To the best of our knowledge the chemical, physical and toxicological properties have not been thoroughly investigated. Specific data is not available. Exercise appropriate procedures to minimize potential hazards. Additional information: RTECS #: TC [Potassium phosphate] Section 12 Ecological Information Toxicity to fish: No data available Toxicity to daphnia and other aquatic invertebrates: No data available Toxicity to algae: No data available Persistence and degradability: No data available Bioaccumulative potential: No data available Mobility in soil: No data available PBT and vpvb assessment: No data available Other adverse effects: An environmental hazard cannot be excluded in the event of unprofessional handling or disposal. Section 13 Disposal Considerations These disposal guidelines are intended for the disposal of catalog-size quantities only. Federal regulations may apply to empty container. State and/or local regulations may be different. Dispose of in accordance with all local, state and federal regulations or contract with a licensed chemical disposal agency. Section 14 Transport Information UN/NA number: Not applicable Shipping name: Not Regulated Hazard class: Not applicable Packing group: Not applicable Reportable Quantity: No Marine pollutant: No Exceptions: Not applicable 2012 ERG Guide # Not applicable Section 15 Regulatory Information A chemical is considered to be listed if the CAS number for the anhydrous form is on the Inventory list. Component TSCA CERLCA (RQ) RCRA code DSL NDSL WHMIS Classification Potassium phosphate Listed Not Listed Not Listed Listed Not Listed Uncontrolled Product Sodium hydroxide Listed 1,000 lbs (454 kg) D002 Listed Not Listed E Section 16 Additional Information The information contained herein is furnished without warranty of any kind. Employers should use this information only as a supplement to other information gathered by them and must make independent determinations of suitability and completeness of information from all sources to assure proper use of these materials and the safety and health of employees. NTP: National Toxicology Program, IARC: International Agency for Research on Cancer, OSHA: Occupational Safety and Health Administration, STOT: Specific Target Organ Toxicity, SE: Single Exposure, RE: Repeated Exposure, ERG: Emergency Response Guidebook. Revision Date: March 31, 2015 Supercedes:

17 SAFETY DATA SHEET GENERAL STORAGE CODE GREEN Section 1 Chemical Product and Company Information Science Take-Out 80 Office Park Way Pittsford, NY CHEMTREC 24 Hour Emergency Phone Number (800) (585) For laboratory use only. Not for drug, food or household use Product Synonyms Section 2 Buffer Solution ph6 Bottle 3 Water Sample (simulated) Hazards Identification This substance or mixture has not been classified at this time according to the Globally Harmonized System (GHS) of Classification and Labeling of Chemicals. Signal word: WARNING Pictograms: None required Target organs: None known GHS Classification: Skin Irritation (Category 3) Eye irritation (Category 2B) Precautionary statement(s): P264: Wash hands thoroughly after handling. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P332+P313: If skin irritation occurs: Get medical attention. P337+P313: If eye irritation persists: Get medical attention. GHS Label information: Hazard statement(s): H316: Causes minor skin irritation. H320: Causes eye irritation. Ca Prop 65 - This product does not contain any chemicals known to the State of California to cause cancer, birth defects, or any other reproductive harm. Section 3 Composition / Information on Ingredients Chemical Name CAS # % EINECS Water % Potassium bipthalate % Sodium hydroxide % Section 4 First Aid Measures INGESTION: Call physician or Poison Control Center immediately. Induce vomiting only if advised by appropriate medical personnel. Never give anything by mouth to an unconscious person. INHALATION: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention. EYE CONTACT: Check for and remove contact lenses. Flush thoroughly with water for at least 15 minutes, lifting upper and lower eyelids occasionally. Get immediate medical attention. SKIN ABSORPTION: Remove contaminated clothing. Flush thoroughly with mild soap and water. If irritation occurs, get medical attention. Section 5 Fire Fighting Measures Suitable Extinguishing Media: Use any media suitable for extinguishing supporting fire. Protective Actions for Fire-fighters: In fire conditions, wear a NIOSH/MSHA-approved self-contained breathing apparatus and full protective gear. Use water spray to keep fire-exposed containers cool. Specific Hazards: During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Section 6 Accidental Release Measures Personal Precautions: Evacuate personnel to safe area. Use proper personal protective equipment as indicated in Section 8. Provide adequate ventilation. Environmental Precautions: Avoid runoff into storm sewers and ditches which lead to waterways. Containment and Cleanup: Absorb with inert dry material, sweep or vacuum up and place in a suitable container for proper disposal. Wash spill area with soap and water. Section 7 Handling and Storage Precautions for Safe Handling: Read label on container before using. Do not wear contact lenses when working with chemicals. Keep out of reach of children. Avoid contact with eyes, skin and clothing. Do not inhale vapors, spray or mist. Use with adequate ventilation. Avoid ingestion. Wash thoroughly after handling. Remove and wash clothing before reuse. Conditions for Safe Storage: Store in a cool, well-ventilated area away from incompatible substances.

18 Section 8 Exposure controls / Personal Protection Exposure Limits: Chemical Name ACGIH (TLV) OSHA (PEL) NIOSH (REL) None None established None established None established Engineering controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower and fire extinguishing material. Personnel should wear safety glasses, goggles, or faceshield, lab coat or apron, appropriate protective gloves. Use adequate ventilation to keep airborne concentrations low. Respiratory protection: None should be needed in normal laboratory handling at room temperatures. If misty conditions prevail, work in fume hood or wear a NIOSH/MSHA approved respirator. Section 9 Physical and Chemical Properties Appearance: Clear, colorless liquid. Evaporation rate ( Water = 1): <1 Odor: No odor. Flammability (solid/gas): Data not available. Odor threshold: Data not available. Explosion limits: Lower/Upper: Data not available ph: 6.0 Vapor pressure (mm Hg): 14 (water) Melting/Freezing point: Approx. 0 C (32 F) (water) Vapor density (Air = 1): 0.7 (water) Boiling point: Approx. 100 C (212 F) (water) Relative density (Specific gravity): Approx. 1.0 (water) Flash point: Data not available Solubility(ies): Complete in water. Section 10 Stability and Reactivity Chemical stability: Stable Hazardous polymerization: Will not occur. Conditions to avoid: Excessive temperatures which cause evaporation. Incompatibilities with other materials: Acids, alkalies, and air will change the buffer s ability. Hazardous decomposition products: Thermal decomposition will yield carbon oxides. Partition coefficient: Data not available Auto-ignition temp.: Data not available Decomposition temp.: Data not available Viscosity: Data not available. Molecular formula: Mixture Molecular weight: Mixture Section 11 Toxicological Information Acute toxicity: Data not available Skin corrosion/irritation: Data not available Serious eye damage/irritation: Data not available Respiratory or skin sensitization: Data not available Germ cell mutagenicity: Data not available Carcinogenity: Data not available NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP. IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Reproductive toxicity: Data not available STOT-single exposure: Data not available Aspiration hazard: Data not available STOT-repeated exposure: Data not available Potential health effects: Inhalation: May be harmful if inhaled. Ingestion: May be harmful if swallowed. Skin: May cause mild irritation. Eyes: May cause mild irritation. Signs and symptoms of exposure: To the best of our knowledge the chemical, physical and toxicological properties have not been thoroughly investigated. Specific data is not available. Exercise appropriate procedures to minimize potential hazards. Additional information: RTECS #: Data not available Section 12 Ecological Information Toxicity to fish: No data available Toxicity to daphnia and other aquatic invertebrates: No data available Toxicity to algae: No data available Persistence and degradability: No data available Bioaccumulative potential: No data available Mobility in soil: No data available PBT and vpvb assessment: No data available Other adverse effects: An environmental hazard cannot be excluded in the event of unprofessional handling or disposal. Section 13 Disposal Considerations These disposal guidelines are intended for the disposal of catalog-size quantities only. Federal regulations may apply to empty container. State and/or local regulations may be different. Dispose of in accordance with all local, state and federal regulations or contract with a licensed chemical disposal agency. Section 14 Transport Information UN/NA number: Not applicable Shipping name: Not Regulated Hazard class: Not applicable Packing group: Not applicable Reportable Quantity: No Marine pollutant: No Exceptions: Not applicable 2012 ERG Guide # Not applicable Section 15 Regulatory Information A chemical is considered to be listed if the CAS number for the anhydrous form is on the Inventory list. Component TSCA CERLCA (RQ) RCRA code DSL NDSL WHMIS Classification Potassium bipthalate Listed Not Listed Not Listed Listed Not Listed Not listed Sodium hydroxide Listed 1,000 lbs (454 kg) D002 Listed Not Listed E Section 16 Additional Information The information contained herein is furnished without warranty of any kind. Employers should use this information only as a supplement to other information gathered by them and must make independent determinations of suitability and completeness of information from all sources to assure proper use of these materials and the safety and health of employees. NTP: National Toxicology Program, IARC: International Agency for Research on Cancer, OSHA: Occupational Safety and Health Administration, STOT: Specific Target Organ Toxicity, SE: Single Exposure, RE: Repeated Exposure, ERG: Emergency Response Guidebook. Revision Date: March 31, 2015 Supercedes:

19 SAFETY DATA SHEET GENERAL STORAGE CODE GREEN Section 1 Chemical Product and Company Information Science Take-Out 80 Office Park Way Pittsford, NY CHEMTREC 24 Hour Emergency Phone Number (800) (585) For laboratory use only. Not for drug, food or household use Product Synonyms Section 2 Buffer Solution ph5 Bottle 4 Water Sample (simulated) Hazards Identification This substance or mixture has not been classified at this time according to the Globally Harmonized System (GHS) of Classification and Labeling of Chemicals. Signal word: WARNING Pictograms: None required Target organs: None known GHS Classification: Skin Irritation (Category 3) Eye irritation (Category 2B) Precautionary statement(s): P264: Wash hands thoroughly after handling. P305+P351+P338: IF IN EYES: Rinse cautiously with water for several minutes. Remove contact lenses, if present and easy to do. Continue rinsing. P332+P313: If skin irritation occurs: Get medical attention. P337+P313: If eye irritation persists: Get medical attention. GHS Label information: Hazard statement(s): H316: Causes minor skin irritation. H320: Causes eye irritation. Ca Prop 65 - This product does not contain any chemicals known to the State of California to cause cancer, birth defects, or any other reproductive harm. Section 3 Composition / Information on Ingredients Chemical Name CAS # % EINECS Water % Potassium bipthalate % Sodium hydroxide % Section 4 First Aid Measures INGESTION: Call physician or Poison Control Center immediately. Induce vomiting only if advised by appropriate medical personnel. Never give anything by mouth to an unconscious person. INHALATION: Remove to fresh air. If not breathing, give artificial respiration. If breathing is difficult, give oxygen. Get medical attention. EYE CONTACT: Check for and remove contact lenses. Flush thoroughly with water for at least 15 minutes, lifting upper and lower eyelids occasionally. Get immediate medical attention. SKIN ABSORPTION: Remove contaminated clothing. Flush thoroughly with mild soap and water. If irritation occurs, get medical attention. Section 5 Fire Fighting Measures Suitable Extinguishing Media: Use any media suitable for extinguishing supporting fire. Protective Actions for Fire-fighters: In fire conditions, wear a NIOSH/MSHA-approved self-contained breathing apparatus and full protective gear. Use water spray to keep fire-exposed containers cool. Specific Hazards: During a fire, irritating and highly toxic gases may be generated by thermal decomposition or combustion. Section 6 Accidental Release Measures Personal Precautions: Evacuate personnel to safe area. Use proper personal protective equipment as indicated in Section 8. Provide adequate ventilation. Environmental Precautions: Avoid runoff into storm sewers and ditches which lead to waterways. Containment and Cleanup: Absorb with inert dry material, sweep or vacuum up and place in a suitable container for proper disposal. Wash spill area with soap and water. Section 7 Handling and Storage Precautions for Safe Handling: Read label on container before using. Do not wear contact lenses when working with chemicals. Keep out of reach of children. Avoid contact with eyes, skin and clothing. Do not inhale vapors, spray or mist. Use with adequate ventilation. Avoid ingestion. Wash thoroughly after handling. Remove and wash clothing before reuse. Conditions for Safe Storage: Store in a cool, well-ventilated area away from incompatible substances.

20 Section 8 Exposure controls / Personal Protection Exposure Limits: Chemical Name ACGIH (TLV) OSHA (PEL) NIOSH (REL) None None established None established None established Engineering controls: Facilities storing or utilizing this material should be equipped with an eyewash facility and a safety shower and fire extinguishing material. Personnel should wear safety glasses, goggles, or faceshield, lab coat or apron, appropriate protective gloves. Use adequate ventilation to keep airborne concentrations low. Respiratory protection: None should be needed in normal laboratory handling at room temperatures. If misty conditions prevail, work in fume hood or wear a NIOSH/MSHA approved respirator. Section 9 Physical and Chemical Properties Appearance: Clear, colorless liquid. Evaporation rate ( Water = 1): <1 Odor: No odor. Flammability (solid/gas): Data not available. Odor threshold: Data not available. Explosion limits: Lower/Upper: Data not available ph: 5.0 Vapor pressure (mm Hg): 14 (water) Melting/Freezing point: Approx. 0 C (32 F) (water) Vapor density (Air = 1): 0.7 (water) Boiling point: Approx. 100 C (212 F) (water) Relative density (Specific gravity): Approx. 1.0 (water) Flash point: Data not available Solubility(ies): Complete in water. Section 10 Stability and Reactivity Chemical stability: Stable Hazardous polymerization: Will not occur. Conditions to avoid: Excessive temperatures which cause evaporation. Incompatibilities with other materials: Acids, alkalies, and air will change the buffer s ability. Hazardous decomposition products: Thermal decomposition will yield carbon oxides. Partition coefficient: Data not available Auto-ignition temp.: Data not available Decomposition temp.: Data not available Viscosity: Data not available. Molecular formula: Mixture Molecular weight: Mixture Section 11 Toxicological Information Acute toxicity: Data not available Skin corrosion/irritation: Data not available Serious eye damage/irritation: Data not available Respiratory or skin sensitization: Data not available Germ cell mutagenicity: Data not available Carcinogenity: Data not available NTP: No component of this product present at levels greater than or equal to 0.1% is identified as a known or anticipated carcinogen by NTP. IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. OSHA: No component of this product present at levels greater than or equal to 0.1% is identified as a carcinogen or potential carcinogen by OSHA. Reproductive toxicity: Data not available STOT-single exposure: Data not available Aspiration hazard: Data not available STOT-repeated exposure: Data not available Potential health effects: Inhalation: May be harmful if inhaled. Ingestion: May be harmful if swallowed. Skin: May cause mild irritation. Eyes: May cause mild irritation. Signs and symptoms of exposure: To the best of our knowledge the chemical, physical and toxicological properties have not been thoroughly investigated. Specific data is not available. Exercise appropriate procedures to minimize potential hazards. Additional information: RTECS #: Data not available Section 12 Ecological Information Toxicity to fish: No data available Toxicity to daphnia and other aquatic invertebrates: No data available Toxicity to algae: No data available Persistence and degradability: No data available Bioaccumulative potential: No data available Mobility in soil: No data available PBT and vpvb assessment: No data available Other adverse effects: An environmental hazard cannot be excluded in the event of unprofessional handling or disposal. Section 13 Disposal Considerations These disposal guidelines are intended for the disposal of catalog-size quantities only. Federal regulations may apply to empty container. State and/or local regulations may be different. Dispose of in accordance with all local, state and federal regulations or contract with a licensed chemical disposal agency. Section 14 Transport Information UN/NA number: Not applicable Shipping name: Not Regulated Hazard class: Not applicable Packing group: Not applicable Reportable Quantity: No Marine pollutant: No Exceptions: Not applicable 2012 ERG Guide # Not applicable Section 15 Regulatory Information A chemical is considered to be listed if the CAS number for the anhydrous form is on the Inventory list. Component TSCA CERLCA (RQ) RCRA code DSL NDSL WHMIS Classification Potassium bipthalate Listed Not Listed Not Listed Listed Not Listed Not listed Sodium hydroxide Listed 1,000 lbs (454 kg) D002 Listed Not Listed E Section 16 Additional Information The information contained herein is furnished without warranty of any kind. Employers should use this information only as a supplement to other information gathered by them and must make independent determinations of suitability and completeness of information from all sources to assure proper use of these materials and the safety and health of employees. NTP: National Toxicology Program, IARC: International Agency for Research on Cancer, OSHA: Occupational Safety and Health Administration, STOT: Specific Target Organ Toxicity, SE: Single Exposure, RE: Repeated Exposure, ERG: Emergency Response Guidebook. Revision Date: March 31, 2015 Supercedes: