1. Photosynthesis and Light. See real-time evidence that light causes photosynthesis to occur!

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1 Y OU LIGHT UP MY LIFE 1. Photosynthesis and Light You Light Up My Life Student Instruction Sheet Challenge See real-time evidence that light causes photosynthesis to occur! Equipment and Materials computer with USB port Small Tripod Base & Rod PASPORT USB interface mineral oil, 10.0 ml PASPORT Dissolved Oxygen (DO) Sensor high-intensity lamp, such as one with a DataStudio software 100-watt fluorescent bulb Buret Clamp ice, cubes, 25.0 g magnetic stir plate and bar beaker, 1-L Erlenmeyer flask, 250-mL dark cloth, 1 m 2 healthy elodea (Anacharis) plants Student Instruction Sheet (common aquarium plants) Student Response Sheet syringe Safety Precautions Keep water away from electrical outlets, the computer, the keyboard, and the PASPORT equipment! Follow standard laboratory safety procedures. The light source used in this lab can get very hot. Do not touch it or you could burn yourself. Student Instruction Sheet 41

2 1. Photosynthesis and Light YOU LIGHT UP MY LIFE Background The series of biochemical events that comprise photosynthesis have been classified into two main sets: the light-dependent reactions and the lightindependent reactions. The set classified as the light-dependent reactions at its most basic level is involved in capturing light energy into energized molecules that are then used in the set of light-independent reactions to fuel the synthesis of sugars from carbon dioxide and water. The light-dependent reactions are so named because light energy is required. The light-independent reactions do not require light; however, they can and do occur under lighted conditions. Predict Before beginning the elab, complete the prediction and vocabulary portions of the Student Response Sheet. Explore Computer Setup 1. Plug the PASPORT USB interface into the computer s port. 2. Plug the Dissolved Oxygen Sensor into the USB interface. This will automatically launch the PASPortal window. To computer 3. Choose the appropriate DataStudio configuration file entitled 01 Photosynthesis+Light CF.ds and proceed with the following instructions. Equipment Setup Note: Configuration files automatically launch the appropriate display(s), sampling rate(s), etc. 1. Wind 2 or 3 sprigs of elodea (Anacharis) plants around in the 250-mL Erlenmeyer flask, and fill the flask to within 2 cm of the top. Note: Use only healthy plants and the water from the container the plants came in to fill the flask. 42 Student Instruction Sheet

3 Y OU LIGHT UP MY LIFE 1. Photosynthesis and Light 2. Secure the Dissolved Oxygen Sensor on the stand with the clamp. Create a water bath using the 1-L beaker and have it sitting on the magnetic stirrer. Place the flask in the water bath. Add some ice cubes to the water bath so the flask stays cool (see setup figure). Dissolved Oxygen Sensor elodea water bath ice cubes magnetic stir bar mineral oil used to seal flask clamp light source magnetic stirrer 3. Adjust the clamp on the stand so the end of the Sensor is positioned just above the stir bar in the flask. Important! Be sure there are no air bubbles trapped on the end of the DO Sensor. 4. Using a syringe filled with mineral oil, cover the top of the water in the flask. You want to create a seal on top of the water so no air can escape. 5. Turn on the stirrer to a moderate speed so water is fully circulated throughout the flask. 6. In DataStudio on the Experiment menu, click Monitor Data. If necessary, wait a few minutes until the dissolved oxygen (DO) concentration stabilizes. 7. Expand the scale of the graph so only a total range of 2 mg/l is visible. Be sure you can see the range in which the data is recording. Note: You may get an error message that states, The scale of the graph has been changed beyond the recommended resolution of the measurement. For this activity, you can ignore this message. Student Instruction Sheet 43

4 1. Photosynthesis and Light YOU LIGHT UP MY LIFE Hint: To rescale an axis of the graph, hover the cursor over the axis until the rescale symbol( ) appears, and click and drag the axis to rescale the axis. To move the range of the axis, hover the cursor over the axis until the hand symbol ( ) appears, and then click and drag the axis to move the visible range. 8. Click the Stop ( ) button to stop monitoring data. Record Data 1. Click the Start ( ) button and record data for 1 minute under ambient light. Note the dissolved oxygen (DO) concentration and record it in your Data Table on the Student Response Sheet. Note: Do not stop the data run. 2. Place the light source as close to the flask as you can and turn it on. Avoid jostling the setup. Record data under intense light for 15 minutes. Note the DO concentration and record it in your Data Table. Note: Do not stop the data run. Note: During this time, add a piece of ice to the water bath occasionally to keep the setup cool. 3. Remove the light source and carefully cover the entire setup with a dark cloth to exclude all light from the plant. Avoid jostling the setup. Record data under no light for 15 minutes. Note the DO concentration, and record it in the Data Table. Stop the data run. Note: If you jar the setup, the sensor will behave erratically until the jarring stops and equilibrium is re-established. 44 Student Instruction Sheet

5 Y OU LIGHT UP MY LIFE 1. Photosynthesis and Light Analyze 1. Locate a straight portion of the graph line that was plotted during exposure to intense light. Select the Smart Tool ( ). Move the cursor to the beginning of the graph where the DO began to increase. Move the cursor to the corner of the Smart Tool and the Delta Tool ( ) will appear. Drag the Delta Tool to the point where the change in DO concentration occurred for a 10-minute period. Record your answer on the Student Response Sheet and answer the questions. Note: If you are still having trouble consult the DataStudio Online Help to find out how to use the Delta Tool. 2. Locate a straight portion of the graph line that was plotted during darkness. Use the Delta Tool of the Smart Tool to find the change in DO concentration that occurred during a 10-minute period. Record your answer on the Student Response Sheet and answer the questions. 3. Save your DataStudio file (on the File menu, click Save Activity As...) to the location specified by your teacher. 4. Answer all questions on the Student Response Sheet. 5. Follow your teacher s instructions regarding cleaning up your work space. Student Instruction Sheet 45

6 1. Photosynthesis and Light YOU LIGHT UP MY LIFE 46 Student Instruction Sheet

7 Y OU LIGHT UP MY LIFE 1. Photosynthesis and Light Student Response Sheet Name: Date: You Light Up My Life Vocabulary Use available resources to find the definitions of the following terms: light-independent reactions (of photosynthesis): dissolved oxygen (DO): light-dependent reactions (of photosynthesis): photosynthesis: Predict What do you think will happen to levels of dissolved oxygen when you shine light on the plant? Student Response Sheet 51

8 1. Photosynthesis and Light YOU LIGHT UP MY LIFE Data Sketch your graph. Annotate which region represents the light on statues as well as the region that represents the light off region. Describe the pattern of the graph as you turn the light onto the plant, after 15 minutes of light exposure, and after 15 minutes when the plant is deprived of light. Minutes from Start Light Condition Dissolved Oxygen Concentration (mg/l) 1 Ambient light 16 End Intense light 31 End Complete Darkness 52 Student Response Sheet

9 Y OU LIGHT UP MY LIFE 1. Photosynthesis and Light Analyze 1. Under high intensity light conditions, what is the rate of increase of DO (per 10 minutes)? mg/l/10 min (per 1 minute)? mg/l/min 2. In darkness, what is the rate of decrease of DO (per 10 minutes)? mg/l/10 min (per 1 minute)? mg/l/min Synthesize 1. Why is it necessary to seal the top of the flask? 2. What is meant by a closed system? Explain how your setup represented a closed system. 3. Why does the concentration of DO increase under high intensity light? 4. Why does the concentration of DO decrease in darkness? Student Response Sheet 53

10 1. Photosynthesis and Light YOU LIGHT UP MY LIFE 54 Student Response Sheet

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