Recovery collection complete sample recovery in preparative HPLC
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1 Recovery collection complete sample recovery in preparative HPLC Presented by Udo Huber Senior Application Chemist Slide 1
2 Contents Introduction Recovery collection Why? Recovery collection How? Recovery collection with the Agilent 1100 Series purification system Basic solutions 12-position/13-port valve Third-party fraction collector and BCD board Agilent solution Agilent 1100 Series recovery collector Funnel tray Recovery location setup and tracking Slide 2
3 Recovery collection Recovery collection: Everything that is not collected as a fraction will not go into waste but into a sample-specific container. Recovery Applications: Walk-up system, chemists get fractions + recovery = everything back For very valuable samples Fractions Slide 3
4 Recovery collection Recovery collection: Recovery of sample Or Gives each sample a dedicated waste container. If required, recovery solution can be evaporated and samle redissolved and re-injected. Eliminates sample entering general waste container from which it is not recoverable. Slide 4
5 Recovery collection Why? Incorrect threshold Possible reasons for non-collection: User inputs wrong target mass/formula User selects inappropriate generic method Peaks fail to cross trigger threshold of generic methods Sample has poor ionization Mechanical/software failure Slide 5
6 Recovery collection How? Fraction collector Detector Diverter valve Waste Fraction container Recovery positions Recovery collection must be done from the waste line of the fraction collector. If more than one fraction collector is in the system, the waste lines must be combined before going to recovery collection. Slide 6
7 Recovery collection with the Agilent 1100 Series purification system 12-position/13-port valve - 12 recovery locations - simple recovery location tracking by software Third-party fraction collector and BCD board - Number of recovery locations depend on fraction collector - no recovery location tracking by software Agilent 1100 Series fraction collector - up to 120 recovery locations with unlimited volume - complete recovery location tracking Slide 7
8 Break Number 1 Slide 8
9 12-position/13-port valve Recovery collection: Waste line of fraction collector is connected to position 13 of the valve Valve positions 1-12 can be used for recovery Slide 9
10 Software control Recovery collection: Valve switches to next recovery position after each run (Next position after run). After 12 samples system must be reset. Slide 10
11 Reporting of recovery position ChemStation report style: Full Slide 11
12 Third-party fraction collector and BCD board BCD board: The BCD board provides a BCD output for the bottle number of the autosampler and four external contacts. The external contact closure contacts are relay contacts. There are general purpose cables available to connect the BCD output and the external contacts to external devices. Slide 12
13 Third-party fraction collector and BCD board Configuration: Installation in the autosampler Installation recognized automatically Set use BCD port for to Binary Output Slide 13
14 Third-party fraction collector and BCD board Slide 14
15 Third-party fraction collector and BCD board Set up: Contact A closed/open to give pulse Contact A was used to move third-party FC to next position Contact B could be used to switch diverter valve of third-party FC Slide 15
16 Hardware Slide 16
17 Break Number 2 Slide 17
18 Agilent 1100 Series fraction collector Agilent fraction collector Complete software control Collection into any fraction container or into funnels Complete reporting of recovery locations (GUI, printed report) Slide 18
19 Agilent 1100 Series fraction collector Column Selection valve Fraction collectors Recovery collector Slide 19
20 Funnel tray The Agilent funnel tray for virtually unlimited recovery volume. Slide 20
21 Fraction collector configuration Slide 21
22 Recovery location setup 1. ChemStation next free recovery location is always used 2. Purify recovery location can be specified A. Setup Study Parameters screen Slide 22
23 Recovery location setup B. Graphical user interface Slide 23
24 Recovery tracking 1. ChemStation report Slide 24
25 Recovery tracking 2. Purify report Slide 25
26 Recovery tracking 3. Purify GUI Slide 26
27 Recovery collection Introduction Recovery collection Why? Recovery collection How? Recovery collection with the Agilent 1100 Series purification system Basic solutions 12-position/13-port valve Third-party fraction collector and BCD board Agilent solution Agilent 1100 Series recovery collector Funnel tray Recovery location setup and tracking Slide 27
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