AB SCIEX QTRAP 4500 System

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1 MAX cover image size 4.8 inches X 7.8 inches In Body page mode, draw a graphic frame to match this section and then go to the next step. Delete this text from the Front Cover Master page. View>Master Pages, and select the frame and delete it. Document Number: Release Date: March 2012

2 This document is provided to customers who have purchased AB Sciex equipment to use in the operation of such AB Sciex equipment. This document is copyright protected and any reproduction of this document or any part of this document is strictly prohibited, except as AB Sciex may authorize in writing. Software that may be described in this document is furnished under a license agreement. It is against the law to copy, modify, or distribute the software on any medium, except as specifically allowed in the license agreement. Furthermore, the license agreement may prohibit the software from being disassembled, reverse engineered, or decompiled for any purpose. Portions of this document may make reference to other manufacturers and/or their products, which may contain parts whose names are registered as trademarks and/or function as trademarks of their respective owners. Any such use is intended only to designate those manufacturers' products as supplied by AB Sciex for incorporation into its equipment and does not imply any right and/or license to use or permit others to use such manufacturers' and/or their product names as trademarks. AB Sciex makes no warranties or representations as to the fitness of this equipment for any particular purpose and assumes no responsibility or contingent liability, including indirect or consequential damages, for any use to which the purchaser may put the equipment described herein, or for any adverse circumstances arising therefrom. For research use only. Not for use in diagnostic procedures. The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners. AB SCIEX is being used under license. AB SCIEX 71 Four Valley Dr., Concord, Ontario, Canada. L4K 4V8. AB Sciex LP is ISO 9001 registered AB SCIEX. Printed in Canada.

3 Contents Chapter 1 Customer and FSE Responsibilities Customer Responsibilities Customer Supplied Solutions and Equipment FSE Responsibilities During Installation Operator Familiarization Technical Support Chapter 2 Site Preparation Tasks Instrument Specifications Operating Environment Mains Supply System Electrical Specifications Protective Conductor Mains Supply Connections Mains Supply Fluctuations UPS (Uninterruptible Power Supply) Gas Supply Optional Gas Generators Ventilation and Waste-Collection System Exhaust Pump Venting Device Requirements Network and Computers Customer Printer Requirements LAN Connection (Optional) Layout Requirements Installation Site Clearances Physical Dimensions Chapter 3 Checklists of 26

4 Contents 4 of 26

5 Customer and FSE Responsibilities 1 This guide is for the personnel responsible for preparing the facility for the installation of the. Note: If the site preparation tasks are not complete when the AB SCIEX FSE (Field Service Employee) arrives, the scheduled installation will be postponed. The information in this section supplements the Checklists on page 21. If you have further questions, contact your AB SCIEX FSE. Customer Responsibilities Carefully inspect the boxes and note any damage or mishandling on the shipping documents. Contact AB SCIEX if the tip or shock indicators on the crates show that the shipment was mishandled during transit. Note: Do not unpack the instrument crate or computer boxes. The AB SCIEX FSE will unpack and move the instrument at the time of the installation. You are responsible for supplying the following: Providing regulators, shut off valves, electrical receptacles, solvents, and gases. Providing all the LC (liquid chromatography) equipment, fittings, and sample tubing required for the LC equipment unless purchased from AB SCIEX. Completing the checklists found in Checklists on page 21 and returning them to the FSE. Providing the required solutions and equipment. For a list of requirements, refer to the following section, Customer Supplied Solutions and Equipment. Customer Supplied Solutions and Equipment Printers, ordered through AB SCIEX LC system, including waste bottles unless ordered through AB SCIEX Pipettors: 20 µl, 100 or 200 µl, 1 ml Polyethylene dilution vials: 20 ml Polyethylene bottles (amber or high density): 60 ml Ammonium acetate: 100 mg HPLC grade or 5 M solution Formic acid: 50 ml Acetonitrile: HPLC or MS grade 2 L Methanol: HPLC or MS grade 2 L 5 of 26

6 Customer and FSE Responsibilities Deionized water: HPLC grade 2 L Table for the computer and monitor. Refer to Layout Requirements on page 17. Instrument bench FSE Responsibilities The FSE will: Supply all fittings, plugs, and cables required to connect the mass spectrometer to the electrical receptacles, and regulators, provided they are within the maximum distances described herein. Test and qualify the system to the specifications in the Installation Guide. Set up LC equipment sold and supported by AB SCIEX. Integrate other supported LC equipment. Set up optional gas generator equipment sold by AB SCIEX only. During Installation After an AB SCIEX FSE unpacks the system (with assistance from people at your site), it takes several hours for the FSE to set up the system and check its operation. When the system reaches proper vacuum, the FSE will perform installation qualification tests. Operator Familiarization During installation, the FSE provides a system and software overview, reviews data, and provides some basic operator familiarization. Training for auxiliary equipment supplied by other manufacturers is not provided by the FSE. Technical Support AB SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughout the world. They can answer questions about the instrument or any technical issues that may arise. For more information, visit the web site at 6 of 26

7 2 Note: The site must not be designated BioSafety Level 3 (BSL-3) or BSL-4. AB SCIEX does not install, service, or repair AB SCIEX instruments in areas designated BSL-3 or BSL-4. Instrument Specifications The equipment pollution degree is Pollution Degree 2. The mains supply transient overvoltage is Overvoltage Category II. For more information, refer to the International Electrotechnical Commission standards IEC and IEC Operating Environment Standard Requirements: An ambient temperature of 15 to 30 C ±2 per hour (46 to 86 F ±3.6 ) Relative humidity from 20 to 80%, non-condensing The instrument and roughing pump generate a combined heat load of 6500 Btu/h Caution: Do not place the roughing pump in an enclosed unventilated area. Otherwise, it will fail prematurely or shut down from overheating and may cause severe damage to the instrument. Note: Ambient temperature fluctuations may result in mass shifts in spectra. Note: Operation of the instrument at altitudes higher than 2000 m (6400 ft.) above sea level may impact instrument performance. Optional Requirements: We recommend that you install a soft wall behind the instrument to reduce the reflection of sound generated by the equipment. Note: Peripheral devices may have different operating environment requirements. Check the operating environment requirements with the vendor of each peripheral device that will be used with the instrument. 7 of 26

8 Sound Pressure Specifications: Table 2-1 Sound Pressure Levels Sound pressure Average sound pressure level Maximum sound pressure level Level 63 dba 65 dba Vibration Specifications: The packaged instrument was tested to confirm the effects of transportation do not impact instrument performance upon arrival at the customer destination. AB SCIEX has no specific data that relates to instrument performance under the effects of vibration. Mains Supply WARNING! Electrical Shock Hazard: Use only qualified personnel for the installation of all electrical supplies and fixtures, and make sure that all installations adhere to local regulations. The total combined system (instrument and roughing pump) power consumption is: Leybold Sogevac SV 28: 2420 VA (50 Hz) or 2250 (60 Hz) at 230 VAC Varian MS 30: 2200 VA (50 or 60 Hz) at 230 VAC Note: The system is configured with a Leybold Sogevac SV 28 or Varian MS 30 roughing pump. You do not need an external line transformer for the instrument, optional bench, or roughing pump. Caution: Do not unpack or connect any components. The AB SCIEX FSE will unpack, connect, and configure the system for the proper operating voltage. 8 of 26

9 System Electrical Specifications The following tables contain the electrical specifications for the instrument, roughing pump, bench, computer, and monitor. Note: Specifications are subject to change without notice. Table 2-2 Instrument Electrical Specifications Instrument Nominal input voltage Input voltage fluctuation Frequency Input current Input power Value 200 to 240 V ±10% of nominal 50/60 Hz 10 A 1000 VA Table 2-3 Leybold Sogevac SV 28 Roughing Pump Electrical Specifications Leybold Sogevac SV 28 roughing pump Nominal input voltage Input voltage fluctuation Frequency Input current Input power Value 200 to 240 V ±10% of nominal 50/60 Hz 4.2 A (50 Hz), 4.7 A (60 Hz) 2420 VA (50 Hz), 2250 (60 Hz) Table 2-4 Varian MS 30 Roughing Pump Electrical Specifications Varian MS 30 roughing pump Nominal input voltage Input voltage fluctuation Frequency Input current Input power Value 200 to 240 V ±10% of nominal 50/60 Hz 1.76 A 1200 VA Table 2-5 Optional Instrument Bench Electrical Specifications Optional instrument bench Value Nominal input voltage 100 to 240 V Input voltage fluctuation ±10% of nominal Frequency 50/60 Hz Max input current 1 A Max input power 240 VA 9 of 26

10 Table 2-6 Computer Electrical Specifications Computer (Optiplex 990) Value Nominal input voltage 115/230 V Input voltage fluctuation ±10% of nominal Frequency 50/60 Hz Max input current 6/3 A Max input power 690 VA Table 2-7 Monitor Electrical Specifications Monitor (U2312HM) Value Nominal input voltage 100 to 240 V Input voltage fluctuation +6/ 10% of nominal Frequency 50/60 Hz Max input current 1.5 A Max input power 360 VA Protective Conductor The mains supply shall include a protective earth conductor installed or checked by a qualified electrician before connecting the instrument. Do not intentionally interrupt the protective conductor. Any interruption of the protective conductor is likely to make the installation dangerous. Mains Supply Connections North America Requirements: Two separate 208 VAC,15A branch circuits, each with duplex receptacles: Total of four outlets. Branch circuits equipped with NEMA 6-15R straight blade receptacles as shown in Figure of 26

11 Figure 2-1 NEMA 6-15 duplex receptacle and plug Optional gas generator requires an additional branch circuit. Consult the manufacturer for specific power requirements. Additional outlets (120 VAC) for computer, monitor, printer, and LC equipment Figure 2-2 System mains supply configuration Item Description 1 One 208 VAC, 15 A branch circuit is required for the instrument.the optional bench can be plugged into the instrument branch circuit. 2 One 208 VAC, 15 A branch circuit is required for the roughing pump. 3 One branch circuit is required for the optional gas generator. 4 Additional 120 V outlets for computer, monitor, printer, and LC equipment as required. 11 of 26

12 International Requirements: Provide mains supply circuits in compliance with local regulations. Mains Supply Fluctuations In areas where the mains supply is subject to voltage fluctuations exceeding ±10% of the nominal value (200 to 240 VAC), a power conditioner may be required. For more information, refer to Table 2-8. Note: Peripheral devices may have different mains supply fluctuation limits. Check the mains supply fluctuation limit with the vendor of each peripheral device that will be used with the instrument. UPS (Uninterruptible Power Supply) Use a pure sine-wave UPS or power conditioner to maintain the system supply (mass spectrometer, computer, monitor, and roughing pump) during power outages. If you power the system from a UPS or power conditioner, it must meet the following specifications. Table 2-8 UPS and Power Conditioner Requirements Condition Requirements Output voltage AC True on line (double conversion) Frequency 50/60 Hz Waveform Pure sine wave Minimum peak current 3 input current Output voltage distortion <3% Output protection Circuit breaker Minimum power requirement 4000 VA 12 of 26

13 Gas Supply Use high-pressure liquid nitrogen dewars or AB SCIEX approved gas generators. Caution: Each supply must be separately regulated at the instrument otherwise over pressure can damage gas controllers. It Figure 2-3 Gas connection configuration Item Gas Description Requirements Tubing 1 Gas 1/Gas 2 Zero air Zero grade air at 100 psi (max.); flow rate of 22 L/min. 1/4 in 2 Curtain Gas supply; CAD gas UHP (ultra high purity) nitrogen (N2) 3 Source exhaust House air, zero air, or UHP N2 UHP N2 at 60 psi (max); flow rate of 10 L/min. House air, zero air, or UHP N2 at 55 psi; flow rate of 8 L/min. Do not operate below 55 psi. 1/4 in 1/4 in 13 of 26

14 Table 2-9 Required Gas, Regulators, and Fittings Gas Gas type Regulators Fitting size Gas 1 and Gas 2 Curtain Gas supply Zero grade air Maximum 105 psi (7.25 bar) Suggested manufacturer 1/4 in Matheson UHP N 2 Maximum 60 psi (4.13 bar) 1/4 in Matheson CAD gas UHP N 2 Maximum 60 psi (4.13 bar) 1/4 in Matheson Source exhaust Zero-grade air, oil-free air or UHP N 2 55 psi to 105 psi (3.79 bar to 7.25 bar) (Do not operate below 55 psi.) 1/4 in Matheson Note: All regulator output fittings are 1/4 inch NPT to 1/4 inch Swagelok (Swagelok P/N SS ). Figure 2-4 Swagelok connection and tubing Optional Gas Generators Gas generators are available from AB SCIEX. Refer to the manufacturer s documentation that comes with the gas generator for system specifications. Ventilation and Waste-Collection WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard: Use only qualified personnel for the installation of plumbing and ventilation fixtures, and make sure that all installations adhere to local regulations. Caution: Do not connect the vent fitting on the drain vessel directly to a negative flow vent. The additional vacuum created by such a connection could trip the source exhaust pump pressure switch, falsely indicating that the source exhaust pump is working when it is not, and enable the instrument electronics. 14 of 26

15 Standard Requirements: Roughing pump: one negative flow vent with 3.2 cm (1 1/4 in) o.d. (outer diameter) smooth fitting located within 1.5 m (5 ft) of the roughing pump. Position the exhaust vent approximately 1 meter above the floor. Source exhaust drain vessel supplied by AB SCIEX: one negative flow vent with 3.2 cm (1 1/4 in) o.d. You also require sufficient length of tubing with 1.3 cm (1/2 in) i.d. (inner diameter) to fit onto the source exhaust drain vessel and loosely couple it to the vent. The laboratory ventilation device should produce an average airflow velocity of 30 linear m/min (100 linear ft/min). The actual amount of airflow rate depends on the cross sectional area of the duct and can be calculated from the following formula: Airflow rate = Velocity Area (cross sectional area of the duct) Make sure the ventilation fixtures installation follows local regulation and biohazard regulations. You must supply exhaust ventilation as required by local regulations. System Exhaust Pump WARNING! Radiation Hazard, Biohazard, Toxic Chemical Hazard, or Reactive Chemical Hazard: Make sure that the source exhaust system is connected to an external fume hood or an external vent, and be sure to use it to remove sample vapor exhaust and pump exhaust from the laboratory environment. Operating the mass spectrometer without adequate ventilation may result in the release of hazardous vapors into the laboratory environment. WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard: Make sure that the laboratory is equipped with an appropriate ventilation system. Ventilation of the laboratory is vital for operator safety. Additional Requirements: Minimize the length and number of bends in the ducting system. Use smooth stainless steel tubing instead of flexible tubing to reduce ventilation system friction loss or drag. Use 45 elbows to turn corners. 15 of 26

16 1 2 3 Figure 2-5 Vent connections Item Description 1 Connection to vent. 2 Connection to roughing pump exhaust. Do not let the tubing sag because it might become blocked. 3 Drain bottle (Drain bottle shown at the back of the instrument to show connection points. The drain bottle is located at the side of the instrument when installed.) Do not let the tubing sag because it might become blocked. Venting Device Requirements It is recommended that the blower used for the exhaust be equipped with an indicator light located near the instrument to indicate to the operator when the blower is on. The length of the duct that enters the blower must be a straight length of at least ten times the duct s diameter. Equip the outlet end of the system with a backdraft damper and an exhaust stack. Keep the exhaust outlet away from open windows or inlet vents. Make up air must be in the same quantity as the air exhausted by the system. An airtight lab causes an efficiency loss in the exhaust system. 16 of 26

17 Network and Computers Make sure that your network or IT specialist is available to install your security software while the AB SCIEX FSE is present. Caution: Other than security software, do not install additional software on the computer. Changes to the configured software could void the instrument warranty and cause the system to stop operating. Customer Printer Requirements The system can be connected to a network or dedicated printer. Make sure that the printer and any necessary print drivers are available before the scheduled installation. LAN Connection (Optional) Make sure that an active, tested LAN connection is in place before the scheduled installation date. Layout Requirements Figure 2-6 to Figure 2-9 show examples of typical configurations. In each of the configurations, the instrument requires a clearance of one meter between any adjacent equipment or wall surface. Figure 2-6 Layout configuration (not to scale) 17 of 26

18 Figure 2-7 Layout configuration (not to scale) Figure 2-8 Layout configuration (not to scale) 18 of 26

19 Figure 2-9 Layout configuration (not to scale) Installation Site Clearances Make sure the installation site meets the required building clearances. Refer to Table Table 2-10 Site Clearances Clearance Front, back, and left sides Measurement Instrument clearance requirement is 1 m for service access. Note: Do not unpack the crates or computer boxes. The AB SCIEX FSE will unpack and move the instrument at the time of the installation. 19 of 26

20 Physical Dimensions Check the weight and dimensions in Table 2-11 and make sure that you can move the system to the installation site. Make sure that the installation site can accommodate the equipment dimensions, weight, and associated clearance. The mass spectrometer has been designed to support 170 lbs (77.5 kg). The bench is designed to support the weight of the instrument plus its associated load, roughing pump, and an additional 170 lbs (77.5 kg). Table 2-11 Weights and Dimensions Equipment Height Width Length Weight Crate 90.2 cm; 35.5 in 94 cm; 37 in 99 cm; 39 in 62 kg; 137 lbs Mass 59 cm; 24 in 79 cm; 32 in 79 cm; 32 in 130 kg; 285 lbs spectrometer Leybold Sogevac SV 28 roughing pump 25.5 cm; 10 in 24 cm; 9.4 in 42.5 cm; 17.3 in 33 kg; 73 lbs Varian MS 30 roughing pump Instrument bench Accessories crate 22 cm; 9 in 30 cm; 12 in 42 cm; 17 in 34 kg; 75 lbs 78 cm; 31 in 100 cm 40 in 84 cm; 34 in 120 kg; 265 lbs cm; 51 in 104 cm; 41 in 132 cm; 52 in 178 kg; 391 lbs 20 of 26

21 Checklists 3 Table 3-1 Materials for Routine Operation Requirements Solutions and equipment. Refer to Customer Supplied Solutions and Equipment on page 5. Polypropylene bottle and pipettors. Tubing fittings required to connect the supported LC equipment to the instrument. Comments Table 3-2 Packaging and Site Layout Checklist Requirements Verified that items on the packing list are those that were ordered. Otherwise, reported to the AB SCIEX FSE discrepancies in the packing list. Received the system and inspected the crates and boxes for mishandling or damage. Reported to the AB SCIEX FSE: Any damage to the crates or boxes. Tip indicators or shock indicators that show evidence of mishandling during transit. The measured building clearances can accommodate the equipment and crate dimensions. If the dimensions exceed building clearances, contact the AB SCIEX FSE. Do not unpack the crate. Comments Table 3-3 Environmental Checklist Requirements An ambient temperature of 15 to 30 C (46 to 86 F). The ambient temperature cannot change more than 2 C (3.6 F) per hour. Comments Note: Peripheral devices may have different operating environment requirements. Check the operating environment requirements with the vendor of each peripheral device that will be used with the instrument. 21 of 26

22 Checklists Table 3-3 Environmental Checklist (Continued) Requirements 20 to 80% relative humidity, non-condensing. Air conditioning provides for a minimum additional heat load of 6500 Btu/h. Refer to Operating Environment on page 7. Comments Table 3-4 Electrical Checklist for the Instrument and Roughing Pump (International) Requirements One branch circuit (200 to 240 V) for the instrument. The optional bench can be connected to the instrument branch circuit. The mains supply socket outlets must be less than 1.6 m (69 in) from the instrument. One branch circuit (200 to 240 V) for the roughing pump. The mains supply socket outlets must be less than 1.6 m (69 in) from the roughing pump. Record the following values. The measured mains voltage over 24 hrs was: High value +%: Low value %: The mains voltage source does not fluctuate more than ±10% from the nominal voltage. Peripheral devices may have different mains supply fluctuation limits. Check the mains supply fluctuation limit with the vendor of each peripheral device that will be used with the instrument. (Optional) A UPS is provided for the system. The UPS must deliver 200 to 240 VAC, 50/60 Hz, 4000 VA (min). (Optional) A power conditioner is provided for the system. It must deliver 200 to 240 VAC, 50/60 Hz, 4000 VA (min). Make sure a qualified electrician determines the appropriate mains supply configuration based on the system electrical specifications found in Table 2-2 Instrument Electrical Specifications on page 9. Comments 22 of 26

23 Checklists Table 3-5 Additional Electrical Checklist for North American Installation Requirements Two separate branch circuits: 15 A 208 VAC, 50/60 Hz. Branch circuits terminated with NEMA 6-15R receptacles. Each branch circuit must have two outlets. Comments Table 3-6 Electrical Checklist for Additional Accessories (Mains Supply) Requirements Computer equipment (computer, monitor, printer): minimum of two mains supply socket outlets. North America: 120 VAC, 60 Hz. Europe: 230 VAC, 50 Hz; Japan: 100 VAC, 50/60 Hz. For optional devices: as necessary for LC pump, autosampler, diode array detector, and gas generator depending on equipment requirements and installation location. For more information, refer to the documentation that comes with the device. Comments 23 of 26

24 Checklists Table 3-7 Gas Requirements Checklist Requirements Materials for Pressurized Gas Operation Gas 1/Gas 2: Zero grade air.* Delivery pressure of 100 psi (max) with flows up to 22 L/min. * or approved AB SCIEX gas generator Curtain Gas supply and CAD gas supply: UHP nitrogen (99.999%).* Delivery pressure up to 60 psi (max) with flows up to 10 L/min. * or approved AB SCIEX gas generator Source exhaust gas: Clean, dry, and oil-free air or nitrogen.* Delivery pressure of 55 psi (max) with flows up to 8 L/min. * or approved AB SCIEX gas generator If an optional wall-mounted gas generator is used, a supply of compressed oil-free air with a delivery pressure of 110 to 130 psi. Liquid nitrogen generators, if used, secured to meet local regulations. Use only regulators and connectors that comply with local regulations. Gas delivery lines installed as required by local regulations. Comments Table 3-8 Ventilation and Waste Collection Checklist Requirements Roughing pump: one negative flow vent with 3.2 cm (1 1/4 in) o.d. smooth fitting located within 1.5 m (5 ft) of the roughing pump. Source exhaust drain vessel supplied by AB SCIEX: one negative flow vent with 3.2 cm (1 1/4 in) o.d. Sufficient length of tubing with 1.3 cm (1/2 in) i.d. to connect the source exhaust drain vessel and loosely couple it to the vent. Maximum flow rate volume of 30 linear m/min (100 linear ft./min) for pump and source exhaust drain vessel combined or static vacuum of 3/4 inch water. Comments 24 of 26

25 Checklists Table 3-8 Ventilation and Waste Collection Checklist (Continued) Requirements Installation of gas piping and ventilation complies with local regulations. Comments Table 3-9 Computer Checklist Requirements Security Software Make sure that your network or IT specialist is available to connect the computer to your LAN while the AB SCIEX FSE is present. You have obtained your network computer name and domain password. Networking An active, tested LAN connection is available. Printer A network printer or a dedicated printer and necessary print drivers are available. Comments Table 3-10 Instrument Bench Requirements A moveable bench for the instrument or a fixed bench with 1 m (3 ft, 4 in) of clearance on three sides for service access. Instrument bench shall meet physical requirements (dimensions and mass) of instrument as listed in Table 2-11 Weights and Dimensions on page 20. A bench for the data acquisition workstation, located within 4 m (13 ft) of the instrument. Make sure that there is 1 m of clearance from the front, back, and left sides. Comments 25 of 26

26 Checklists 26 of 26

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