1 Physiology Lessons for use with the Biopac Student Lab Lesson 12 New Procedure PULMONARY FUNCTION I Volumes and Capacities For Windows 98SE, Me, 2000 Pro, XP or Mac OS X Richard Pflanzer, Ph.D. Associate Professor Indiana University School of Medicine Purdue University School of Science J.C. Uyehara, Ph.D. Biologist BIOPAC Systems, Inc. William McMullen Vice President BIOPAC Systems, Inc. Manual Revision BIOPAC Systems, Inc. 42 Aero Camino, Goleta, CA (805) , Fax (805) Web Site: BIOPAC Systems, Inc.
2 Page 2 Biopac Student Lab New Lesson Procedure The Lesson 12 procedure was changed based on feedback from instructors. BSL Windows (with journal date 10/27/2005) and BSL Mac incorporate an updated breathing procedure and new analysis instructions to help students obtain accurate Tidal Volume values. OLD OVERVIEW: This recording shows normal breathing for 3 breaths, full inhale, return to normal breathing, full exhale, then a return to normal breathing. REVISED TO: This recording shows normal breathing for 5 breaths, full inhale, full exhale, then a return to normal breathing. Segment 1 Guide: OLD: After the recording begins, Subject will do the following: Breathe normally for 3 breaths, then inhale as deeply as possible, then return to normal breathing for 3 breaths. Next, breathe out completely, then normally for 3 breaths. Recorder should click on the 'Stop' button when Subject is finished. REVISED TO: After the recording begins, Subject will do the following: Breathe normally for 5 breaths, then inhale as deeply as possible, then breathe out completely, then breath normally for 5 breaths. Recorder should click on the 'Stop' button when Subject is finished. Segment 2 Guide: OLD: Subject will breathe normally for 3 breaths, then inhale as deeply as possible, then return to normal breathing for 3 breaths. Next, Subject will exhale completely, then breathe normally for 3 breaths. Keep the Airflow transducer upright at all times. When Subject is finished, Recorder should click on 'Stop.' REVISED TO: Subject will breathe normally for 5 breaths, then inhale as deeply as possible, then exhale completely, then breathe normally for 5 breaths. Keep the Airflow transducer upright at all times. When Subject is finished, Recorder should click on 'Stop.' Analysis Analysis revised to match recorded data. I. INTRODUCTION The introductory text has not changed from the previously supplied lesson, but Fig was updated: Figure 12.2 Example of respiratory volumes and capacities
3 Lesson 12: Pulmonary Function I Page 3 II. EXPERIMENTAL OBJECTIVES The objectives have not changed from the previously supplied lesson. III. MATERIALS The materials have not changed from the previously supplied lesson. IV. EXPERIMENTAL METHODS Key to Symbols The symbols below are used to prompt you throughout Experimental Methods and Data Analysis. If you encounter a problem or need further explanation of a concept, refer to the Orientation Chapter for more details. The data collected in the associated step needs to be recorded in the Data Report (in the section indicated by the alpha character). You can record the data individually by hand or choose Edit > Journal > Paste measurements to paste data to your journal for future reference. You need to manually insert an event marker and key in a label similar to the text in quotes. You insert an event marker during acquisition by pressing F9 and can insert or edit markers and labels once acquisition is completed. Each section of this lesson is presented in a two-column format, as described below. FAST TRACK STEPS This side of the lesson (left, shaded column) is the FAST TRACK through the lesson, and contains a basic explanation of each step. DETAILED EXPLANATION OF STEPS This side of the lesson contains more detailed information to clarify the steps and/or concepts in the FAST TRACK, and may include reference diagrams, illustrations, and screen shots. A. SET UP FAST TRACK SET UP 1. Turn your computer ON. 2. Make sure the BIOPAC MP35/30 unit is turned OFF. 3. Plug the airflow transducer (SS11LA) into Channel Turn on the MP35/30 Data Acquisition Unit. DETAILED EXPLANATION OF SET UP The desktop should appear on the monitor. If it does not appear, ask the laboratory instructor for assistance. BIOPAC MP3X unit Plugs into CH1 SS11LA Airflow Transducer Set Up continues Fig BIOPAC Systems, Inc.
4 Page 4 Biopac Student Lab 5. Place a filter onto the end of the calibration syringe. The filter is required for calibration and recording because it forces the air to move smoothly through the transducer. This assembly can be left connected for future use. You only need to replace the filter if the paper inside the filter tears. The bacteriological filter must be used between the transducer and calibration syringe in order for the data to be accurate. 6. Insert the Calibration Syringe/Filter Assembly into the airflow transducer (Fig. 12.4). IMPORTANT! Always insert on the side labeled Inlet Fig If using SS11LA transducer with removable, cleanable head, always insert syringe assembly on the transducer side labeled Inlet so that the transducer cable exits on the left, as shown in Fig If using SS11L transducer with non-removable head, insert syringe assembly into the larger diameter port. 7. Start the Biopac Student Lab program. 8. Choose Lesson 12 (L12-LUNG-1). 9. Type in your filename. 10. Click OK. IMPORTANT: If your lab sterilizes the airflow heads after each use, make sure a clean head is installed now. Use a unique identifier. This ends the Set Up procedure. END OF SET UP
5 Lesson 12: Pulmonary Function I Page 5 B. CALIBRATION The calibration procedure establishes the hardware s internal parameters (such as gain, offset, and scaling) and is critical for optimum performance. Pay close attention to the entire calibration procedure. FAST TRACK Calibration 1. Pull the Calibration Syringe Plunger all the way out and hold the Calibration Syringe/Filter Assembly upright (Fig. 12.5). NOTE: If you have previously calibrated the SS11LA Airflow Transducer for Lesson 12 or 13, and have not exited the BSL software or changed the transducer, BSL has saved the prior calibration data. - Go to Step 1 of the Recording Data section of this lesson if you wish to begin recording data. - Click Redo Calibration if you wish to recalibrate the transducer. DETAILED EXPLANATION OF CALIBRATION STEPS The Airflow Transducer is sensitive to gravity so it needs to be held upright throughout the calibration and recording. Airflow Transducer hangs freely off the end Airflow transducer held upright at all times Fig Calibration Syringe Plunger Correct placement of hands IMPORTANT: Never hold onto the airflow transducer handle when using the calibration syringe or the syringe tip may break. 2. Click Calibrate. The first calibration procedure stage will run for 8 seconds and end with an alert box. 3. Prepare for the second calibration stage. IMPORTANT: Leave the plunger extended and hold the assembly steady and upright during the entire calibration procedure. Do not touch the plunger because any pressure at this stage will cause inaccurate results. Stage 1 of the calibration procedure ends with an alert box asking if you have read the directions in the journal. Read the directions in Step 5 and/or the journal so that, once the second calibration procedure starts, you understand the procedures. 4. Click Yes after reading the alert box. Do not click on the Yes button until you are completely ready to proceed. When you click Yes, the second stage of the calibration procedure will begin, and will run until you click End Calibration. 5. Cycle the syringe plunger in and out completely 5 times (10 strokes). 6. Click End Calibration. Hold the syringe assembly as shown in Fig above. Use a rhythm of about 1 second per stroke with 2 seconds rest between strokes, i.e., push the plunger in for approximately 1 second, wait 2 seconds, pull the plunger out, wait 2 seconds, and repeat 4 more times. Click End Calibration when done. Note: The calibration procedure is required because of the complexity of the Airflow to Volume calculation. The accuracy of this conversion is aided by analyzing the airflow variations occurring over five complete cycles of the calibration syringe. Additional cycles would also help, but only with diminishing return rates. Five cycles turns out to be an adequate number of cycles for accurate calibration. Calibration continues BIOPAC Systems, Inc.
6 Page 6 Biopac Student Lab 7. Check your calibration data. At the end of the calibration recording, your screen should resemble Fig If correct, go to the Data Recording section. If incorrect, Redo Calibration. END OF CALIBRATION Figure 12.6 If your data shows 5 downward deflections and 5 upward deflections, you may proceed to the Data Recording section. If the data shows any large spikes, then you must redo calibration by clicking Redo Calibration and repeating the entire calibration sequence.
7 Lesson 12: Pulmonary Function I Page 7 C. RECORDING LESSON DATA FAST TRACK Recording DETAILED EXPLANATION OF RECORDING STEPS 1. OPTIONAL: Validate Calibration: a) Click Record. b) Cycle the AFT6 syringe plunger in and out completely 5 times (10 strokes). c) Click Stop. d) Measure P-P on CH2 Volume to confirm the result is 0.6 liters (Fig. 12.7) e) Click Redo and proceed with Subject recording (or click Done and repeat calibration if necessary). 2. Prepare for the recording. IMPORTANT! Subject must be relaxed to obtain accurate measures. Figure 12.7 Calibration Validation shows P-P result 0.6 liters In order to work efficiently, read this entire section so you will know what to do for each recording segment. Following the procedure precisely is very important, as the calculation from airflow to volume is very sensitive. Subject should be seated, facing away from the computer monitor, relaxed, with eyes closed while you review the lesson. Check the last line of the journal and note the total amount of time available for the recording. Stop each recording segment as soon as possible so you don t use an excessive amount of time (time is memory). Note: Residual Volume (RV) cannot be determined using a normal spirometer or airflow transducer, so the Biopac Student Lab software uses a default of 1 Liter. If 1 Liter of RV is not desired, see your Instructor to change the Preference. Hints for obtaining optimal data: a) Subject should be seated, facing away from the computer monitor, relaxed, with eyes closed. b) Subject should insert mouthpiece and begin breathing normally BEFORE the recording is started since the mouthpiece may influence normal values. c) Always insert on and breathe through the transducer side labeled Inlet. d) Keep the Airflow Transducer upright at all times (Fig ). e) A breath is considered a complete inhale-exhale cycle. If you start the recording on an inhale, try to end on an exhale, and viceversa. This is not absolutely critical, but does increase the accuracy of the Airflow to Volume calculation. Recording continues BIOPAC Systems, Inc.
8 Page 8 3. Insert a clean mouthpiece (and filter if applicable) into the airflow transducer as described below: To be safe, follow this procedure precisely to make sure the airflow transducer is sterile. If using the SS11LA transducer and sterilizing the head after each use: Biopac Student Lab IMPORTANT: If your lab sterilizes the airflow heads after each use, make sure a clean head is installed now. Have the Subject personally remove the filter and mouthpiece from the plastic packaging. This mouthpiece will become the Subject s personal mouthpiece. It is advisable to write the Subject s name on the mouthpiece and filter with a permanent marker so they can be reused later. If using SS11LA transducer and sterilizing the head after each use, insert a disposable mouthpiece (BIOPAC AFT2) or an autoclavable mouthpiece (BIOPAC AFT8) into the airflow transducer on the side labeled Inlet. IMPORTANT! Always insert on the side labeled Inlet If using the SS11LA transducer and not sterilizing the head after each use: Fig SS11LA with sterilized head If using SS11LA transducer and not sterilizing the head after each use, insert a filter and mouthpiece into the airflow transducer on the side labeled Inlet. IMPORTANT! Always insert on the side labeled Inlet If using the SS11L: 4. Subject should place his/her personal nose clip on nose. Fig SS11LA with unsterilized head If using SS11L transducer with non-removable head, insert a new filter and mouthpiece into the larger diameter port. Disposable Disposable mouthpiece Bacteriological (AFT2) Filter (AFT1) Airflow Transducer (SS11L) Fig SS11L Recording continues
9 Lesson 12: Pulmonary Function I Page 9 5. Breathe normally for 20 seconds through the Airflow Transducer BEFORE clicking Record. A breath is considered a complete inhale-exhale cycle. Subject should be relaxed with eyes closed for normal breathing. Allow time for Subject to acclimate to the mouthpiece BEFORE clicking Record. IMPORTANT! Subject must remain relaxed and always breathe through the side labeled Inlet 6. Click Record. a) Breathe normally for 5 breaths. b) Inhale as deeply as you can. c) Exhale as deeply as you can. d) Breathe normally for 5 breaths. Fig Keep the Airflow Transducer upright at all times For accurate measures, the Subject must be completely relaxed, with eyes closed, and breathing normally. The mouthpiece will influence the Subject s breathing, so allow time for the Subject to acclimate to the mouthpiece BEFORE clicking Record. A breath is considered a complete inhale-exhale cycle. Subject should be relaxed with eyes closed and not facing the computer. If you start the recording on an inhale, try to end on an exhale, and vice-versa. 7. Click Stop. As soon as the Stop button is pressed, the Biopac Student Lab software will automatically calculate volume data based on the recorded airflow data. At the end of the calculation, both waveforms will be displayed on the screen (Fig ). 8. Review the data on the screen. If correct, go to Step 7. Fig. Fig Your data should resemble Fig.12.12, showing a positive spike for inhalation and a negative spike for exhalation. If incorrect, click Redo. Recording continues The data would be incorrect if you feel you didn t follow the procedure precisely, i.e. you coughed or air escaped. In this case, you should redo the recording by clicking Redo and repeating Steps 5-8. Note that once you press Redo, the data you have just recorded will be erased. BIOPAC Systems, Inc.
10 Page 10 Biopac Student Lab 9. Click Done. After you press Done, your data will automatically be saved in the specified Data Files folder. A pop-up window with options will appear. Make your choice, and continue as directed. END OF RECORDING If choosing the Record from another Subject option: a) You will not need to recalibrate the airflow transducer. For this reason, we recommend that all recordings be completed before you proceed to Data Analysis. b) Remember to have each person use his/her own mouthpiece, bacterial filter and nose clip. c) Repeat Recording Steps 1-7 for each new Subject. d) Each person will need to use a unique file name.
11 Lesson 12: Pulmonary Function I Page 11 V. DATA ANALYSIS FAST TRACK Data Analysis 1. Enter the Review Saved Data mode and choose the correct file. Note channel number (CH) designations: Channel CH 1 CH 2 Displays Airflow Volume DETAILED EXPLANATION OF DATA ANALYSIS STEPS Enter the Review Saved Data mode. Fig Note: Airflow (Channel 1) and Volume (Channel 2) data are shown simultaneously. First, concentrate on the Volume data. 2. Turn OFF Channel 1, Airflow. To toggle a channel ON/OFF, click on the channel number box Optional: Review Airflow data before turning Channel 1 off. 3. Set up the measurement boxes as follows: Channel CH 2 CH 2 CH 2 CH 2 Measurement P-P Max Min Delta and hold down the Ctrl key. The Airflow data does not have a lot of meaning for this lesson and may confusing at first glance, but it contains an interesting perspective on the recording. Note that the vertical scale of the airflow waveform is in Liters per second (Liters/sec.), and that the data is centered on zero. Looking at the graph, you can see that with each exhale, a downward pointing curve appears. The deeper an inhale, the larger the positive peak; the more forceful an exhale, the larger the negative peak. The measurement boxes are above the marker region in the data window. Each measurement has three sections: channel number, measurement type, and result. The first two sections are pull-down menus that are activated when you click on them. The following is a brief description of these specific measurements. P-P: finds the maximum value in the selected area and subtracts the minimum value found in the selected area. Max: displays the maximum value in the selected area. Min: displays the minimum value in the selected area. Delta: computes the difference in amplitude between the last point and the first point of the selected area. The selected area is the area selected by the I-Beam tool (including the endpoints) Data Analysis continues BIOPAC Systems, Inc.
12 Page 12 Biopac Student Lab 4. Review the measurements described in the Introduction to identify the appropriate selected area for each: Total Lung Capacity Tidal Volume Inspiratory Reserve Volume Expiratory Reserve Volume Vital Capacity Expiratory Capacity Inspiratory Capacity Functional residual Capacity Residual Volume 5. Measure observed VC (P-P). A Fig Measurement areas for respiratory volumes and capacities The P-P measurement can be used to obtain VC (Fig ). 6. Take two measures for an averaged TV calculation: a) Use the I-beam cursor to select the inhalation of cycle 3 and note the P-P result (Fig ). The selected area should be from the valley to the peak of the third cycle. B Figure Example of VC from P-P measure The P-P measurement in Fig represents the first value required for the averaged TV calculation. Fig Inhalation of third breath cycle selected to measure P-P Data Analysis continues
13 Lesson 12: Pulmonary Function I Page 13 b) Use the I-beam cursor to select the exhalation of cycle 3 and note the P-P result (Fig ). The selected area should be from the peak to the valley of the third cycle. B The P-P measurement in Fig represents the second value required for the averaged TV calculation. Fig Exhalation of third breath cycle selected to measure P-P 7. Use the I-beam cursor and measurement tools to observe the following volumes and capacities (defined in Fig ): The Delta measurement can be used to obtain IRV, ERV, and other measurements (Fig ). a) IRV (Delta) b) ERV (Delta) c) RV (Min) d) IC (Delta) e) EC (Delta) f) TLC (Max) B Fig Example of measurements for TLC (Max result), RV (Min result), and IRV (Delta result)t 8. Save or Print the data file. 9. Exit the program. You may save the data to a drive, save notes that are in the journal, or print the data file. END OF DATA ANALYSIS END OF LESSON 12 Complete the Lesson 12 Data Report that follows. BIOPAC Systems, Inc.
14 Biopac Student Lab Page 14 Lesson 12 PULMONARY FUNCTION I Volumes and Capacities DATA REPORT Student s Name: Lab Section: Date: Subject Profile Name Age Gender: Male / Female Height Weight I. Measurements A. Vital Capacity i ) Predicted: Use the equation below to calculate your Predicted Vital Capacity: A1 liters Equations for Predicted Vital Capacity Male V.C. = 0.052H A 3.60 Female V.C. = 0.041H A 2.69 Where V.C. H A Vital Capacity in liters Height in centimeters Age in years ii) Observed: Use the P-P measurement result to note Observed Vital Capacity: A2 iii) Observed vs. Predicted What is the Subject s observed Vital Capacity to predicted Vital Capacity as a percentage? Observed VC /Predicted VC = A1/A2 = liters x 100= % liters Note: Vital capacities are dependent on other factors besides age and height. Therefore, 80% of predicted values are still considered normal.
15 Lesson 12: Pulmonary Function I Data Report Page 15 B. Volume & Capacity Measurements Complete Table 12.2 with the requested measurement results and calculate results per the formulas provided. Table 12.2 Measurements Title Measurement Result Calculation Tidal Volume TV a = P-P Cycle 3 inhalation: (a + b) / 2 = b = P-P Cycle 3 exhalation: Inspiratory Reserve Volume IRV Delta Expiratory Reserve Volume ERV Delta Residual Volume RV Min Default = 1 (Preference setting) Inspiratory Capacity IC Delta TV + IRV = Expiratory Capacity EC Delta TV + ERV = Functional Residual Capacity FRC ERV + RV = Total Lung Capacity TLC Max IRV + TV + ERV + RV = C. Observed vs. Predicted Volumes Using data obtained for Table 12.2, compare the Subject s lung volumes with the average volumes presented in the Introduction. Table 12.3 Average Volumes vs. Measured Volumes Volume Title Average Volume Measured Volume Tidal Volume TV Resting subject, normal breathing: greater than TV is approximately 500 ml. equal to During exercise: TV can be more than 3 liters less than Inspiratory Reserve Volume IRV Resting IRV for young adults is greater than males = approximately 3,300 ml equal to females = approximately 1,900 ml less than Expiratory Reserve Volume ERV Resting ERV for young adults is males = approximately 1,000 ml females = approximately 700 ml greater than equal to less than BIOPAC Systems, Inc.
16 Page 16 Biopac Student Lab II. QUESTIONS D. Why does predicted vital capacity vary with height? E. Explain how factors other than height might affect lung capacity. F. How would the volume measurements change if data were collected after vigorous exercise? G. What is the difference between volume measurements and capacities? H. Define Tidal Volume. I. Define Inspiratory Reserve Volume. J. Define Expiratory Reserve Volume. K. Define Respiratory Volume. L. Define Pulmonary Capacity. M. Name the Pulmonary Capacities. End of Lesson 12 Data Report
Lung Volumes and Capacities Experiment 19 Measurement of lung volumes provides a tool for understanding normal function of the lungs as well as disease states. The breathing cycle is initiated by expansion
Human Respiration Laboratory Experiment By Alison L., Thurow, Brittany Baierlein, Rachel C. Holsinger and Robin L. Cooper Department of Biology, University of Kentucky, Lexington, KY 40506 0225, USA. Purpose:
66 LAB 7 HUMAN RESPIRATORY LAB Assignments: Due before lab: Quiz: Three Respiratory Interactive Physiology Animations pages 69 73. Complete the charts on pgs. 67 and 68 and read directions for using BIOPAC
Experiment HE-5: Resting Metabolic Rate (RMR) Before Starting 1. Read the procedures for the experiment completely before beginning the experiment. Have a good understanding of how to perform the experiment
Experiment HE-5: Resting Metabolic Rate (RMR) Before Starting 1. Read the procedures for the experiment completely before beginning the experiment. Have a good understanding of how to perform the experiment
Vapor Pressure of Liquids Computer 10 In this experiment, you will investigate the relationship between the vapor pressure of a liquid and its temperature. When a liquid is added to the Erlenmeyer flask
Application Note CB 06/2016 EasyWarePro Message Numbers Important note: this application note is only valid beginning from EOPC V126.96.36.199 and EOP / Lab V188.8.131.52. Compared to the previous released version
Boyle s Law: Pressure-Volume Relationship in Gases The primary objective of this experiment is to determine the relationship between the pressure and volume of a confined gas. The gas we will use is air,
Exploring the Properties of Gases Computer 30 The purpose of this investigation is to conduct a series of experiments, each of which illustrates a different gas law. You will be given a list of equipment
Armfield Distillation Column Operation Guidelines 11-2016 R.Cox Safety SAFETY GLASSES ARE REQUIRED WHEN OPERATING THE DISTILLATION COLUMN Wear gloves when mixing alcohol feedstock The column will become
Name Class Date Chapter 37 Circulatory and Respiratory Systems Measuring Lung Capacity Introduction The amount of air that you move in and out of your lungs depends on how quickly you are breathing. The
1 Physics 1021 Buoyancy 2 Buoyancy Apparatus and Setup Materials Force probe 1000 ml beaker Vernier Calipers Plastic cylinder String or paper clips Assorted bars and clamps Water Attach the force probe
PRESSURE-TEMPERATURE RELATIONSHIP IN GASES LAB PS2.PALM INTRODUCTION Gases are made up of molecules that are in constant motion and exert pressure when they collide with the walls of their container. The
Objective The purpose of this activity is to analyze the relationship between the pressure and volume of a confined gas at constant temperature, create a hypothesis and proceed to test it using the Labdisc
Diver-Office Getting Started Guide 2007, Schlumberger Water Services Copyright Information 2007 Schlumberger Water Services. All rights reserved. No portion of the contents of this publication may be reproduced
Experiment 6 When a juggler tosses a ball straight upward, the ball slows down until it reaches the top of its path. The ball then speeds up on its way back down. A graph of its velocity vs. time would
1516L Experiment 2 SOLUBILITY OF A SOLID IN WATER Objectives In this experiment you will determine the solubility of potassium nitrate (KNO 3 ) in water at various temperatures. You will prepare a plot
SENSUS PRO MANAGER (for SENSUS or SENSUS PRO devices) User s Guide Palm OS Version 2.0 Published October 17, 2002 2002 ReefNet Inc. 1.0 Introduction The SENSUS PRO data recorder captures the time, depth,
1 HIGHLIGHTS High performance and advanced features target the ICU environment Utilizes an internal blower with a specially designed flow control valve removing the need for using compressed air without
Aerobic Respiration Computer 17 Aerobic cellular respiration is the process of converting the chemical energy of organic molecules into a form immediately usable by organisms. Glucose may be oxidized completely
Question 1: Define vital capacity. What is its significance? Vital capacity is the maximum volume of air that can be exhaled after a maximum inspiration. It is about 3.5 4.5 litres in the human body. It
Some starting precautions: 1. Vacuum filter all buffers. Removes any large particles/debris that may clog your column De-gases the buffers 2. Clarify lysates first by centrifugation and then filtration
Measuring Lung Capacity 1 Name Measuring Lung Capacity Background Information: We need a constant supply of oxygen in order to stay alive. We use oxygen to break down food to release energy and produce
Homeostasis and Negative Feedback Concepts and Breathing Experiments 1 I. Homeostasis and Negative Feedback Homeostasis refers to the maintenance of relatively constant internal conditions. For example,
Cardiovascular and respiratory adjustments to exercise Additional notes on breathing and use of respiratory belt and pulse transducers Notes on breathing The metabolic activities of tissues use up oxygen
Thermal Profiling the Reflow Process The Nomadics TCProfile system is a cost-effective instrument to measure the temperature characteristics of any process where the thermal profile is important to the
Cover Page for Lab Report Group Portion Boundary Layer Measurements Prepared by Professor J. M. Cimbala, Penn State University Latest revision: 30 March 2012 Name 1: Name 2: Name 3: [Name 4: ] Date: Section
Measuring Lung Capacity Background: The amount of air that you move in and out of your lungs while breathing normally is referred to as TIDAL VOLUME. While it is possible to inhale and exhale more forcefully
KEM Scientific, Inc. Instruments for Science from Scientists J-KEM Scientific, Inc. 6970 Olive Blvd. St. Louis, MO 63130 (314) 863-5536 Fax (314) 863-6070 E-Mail: email@example.com Precision Vacuum Controller,
Operation of Oxylog 3000 ventilator Overview Suitable for patients from approximately 7Kg body weight upwards (smallest tidal volume 50mls). Entirely power dependant- will not ventilate if no available
PC Configuration software for Discovery MkVI v 1.03 User guide This user guide describes the different features included in PC Config software, version 1.03, and how they are used. When referring to this
Dissolved Oxygen in Water Computer 12A Although water is composed of oxygen and hydrogen atoms, biological life in water depends upon another form of oxygen molecular oxygen. Oxygen is used by organisms
JETFIRST 150 RTA SYSTEM OPERATING MANUAL Version: 2 Feb 2012 UNIVERSITY OF TEXAS AT ARLINGTON Nanofabrication Research and Teaching Facility TABLE OF CONTENTS 1. Introduction....2 1.1 Scope of Work.....2
New Cortex Software User Guide Initial Set Up Turn on the Cortex and leave to warm up for approximately 15 minutes before calibrating Load MSS Toolbox from the desktop From the toolbox menu select Calibration
Handicap System Overview 1 The Handicap System...1 Creating a Roster...1 Entering Courses...2 Entering Golfers...3 Entering Scores...4 Viewing Scores...7 Combing two nine holes scores to form an 18 hole
Quantitative Analysis of Hydrocarbons by Gas Chromatography Introduction Gas-liquid chromatography (GLC) accomplishes a separation by partitioning solutes between a mobile gas phase and a stationary liquid
SDM-2012 Docking Station Standalone Configuration Operator s Manual Part Number: 71-0254RK Revision: P5 Released: 10/5/12 www.rkiinstruments.com Warranty RKI Instruments, Inc. warrants gas alarm equipment
Chem 366-3 Page XI - 1 EXPERIMENT XI INFRARED SPECTRUM OF SO2 (S&G, 5th ed. Expt 36, 6th ed. Expt. 35) 1. Pre-Lab preparation. The description of this experiment has disappeared from the more recent editions
Viva TPS October 2010 TS11/15 Total Stations Summary Leica builds total stations to the highest quality and calibrates each instrument before it leaves the Factory. After the instrument is shipped or used
USER GUIDE WELCOME TO THE REVOLUTION THANK YOU FOR CHOOSING THE GCQUAD We listened to what you wanted - and created the most accurate, versatile and game-enhancing ball and club analysis solution available
AIR FLOW ANEMOMETER INSTRUCTION MANUAL Thank you for purchasing our company Air Flow Anemometer. This manual provides relative information on how to use the Air Anemometer and warning in operation Please
Akcelik & Associates Pty Ltd PO Box 1075G, Greythorn, Vic 3104 AUSTRALIA ABN 79 088 889 687 For all technical support, sales support and general enquiries: support.sidrasolutions.com SIDRA INTERSECTION
PERFORMANCE EVALUATION #34 NAME: 7200 Ventilator Set Up DATE: 1. **Identify and name the filters on the 7200ae. 2. **Explain how each filter is sterilized. 3. **Trace the gas flow through the ventilator
Circulation and Respiration: Vital Signs Student Version In this lab, you will learn about the circulatory and respiratory systems. You will test the capacity of your lungs, measure your blood pressure
1516L Experiment 1 SOLUBILITY OF A SOLID IN WATER Objectives In this experiment you will determine the solubility of potassium nitrate (KNO 3 ) in water at various temperatures. You will prepare a plot
Air Quality 2b - PFGC/FID Analysis of Benzene and Toluene in Automobile Emissions Introduction: There is a bit of explanation of the health effects of benzene in the Introduction section of the first part
Inhaltsverzeichnis Window and Door Measurement System Air Permeability Test BlowerDoor GmbH Version: TECLOG2 for Windows January 2011 BlowerDoor GmbH März 2009 1 Instructions Window and Door Measurement
DIVE MEET SCORING INSTRUCTIONS Day before meet Physical set up Set up registration/scoring table #1 on 1 meter side and scoring table #2 on 3 meter side of diving well, judges chairs, and award stand as
Lab 4: Transpiration Water is transported in plants, from the roots to the leaves, following a decreasing water potential gradient. Transpiration, or loss of water from the leaves, helps to create a lower
Multifunction Altimeter/Variometer AV1 Revision#3.0, 21/11/2014 For firmware version 2.2 Page intentionally left blank SECTIONS MECHANICAL INSTALLATION ELECTRICAL INSTALLATION USE OF THE INSTRUMENT INSTRUMENT
Chem 2115 Experiment # 8 The Ideal Gas Constant OBJECTIVE: This experiment is designed to provide experience in gas handling methods and experimental insight into the relationships between pressure, volume,
Y OU LIGHT UP MY LIFE 1. Photosynthesis and Light You Light Up My Life Student Instruction Sheet Challenge See real-time evidence that light causes photosynthesis to occur! Equipment and Materials computer
Online League Management lta.tournamentsoftware.com User Manual Further support is available online at www.lta.org.uk/leagueplanner Contents Welcome... 3 Using this guide... 3 Further support?... 3 Publishing
Calibration Guide Model SM/PL Series Precision Loads Anritsu Company 490 Jarvis Drive Morgan Hill, CA 95037-2809 USA Part Number: 10100-00056 Revision: B Published: January 2010 Copyright 2009-2010 Anritsu
Product Calibration Procedure For TriStar II 3020 APPROVALS: Director, Engineering Stefan Koch Manager, Product Integrity and Performance Tony Thornton Senior Project Manager Patrick Wommack Manager, International
User Manual ENGLISH Pages 1-18 FRANÇAIS Pages 19-35 ESPAÑOL Páginas 36-52 DEUTSCH Seiten 53-69 LIT# LD2-160212 LD2-Full_Manual Rev18 Thank you for choosing the GolfBuddy LD2. The GolfBuddy LD2 is the first
! Read this first Zetasizer nano series Self installation and Quick start guide I N S T R U M E N T S MRK825-02 Zetasizer Nano series Self installation and Quick start guide MAN0383 Issue 1.1 July 2007
Respiration - Human 1 At the end of the lectures on respiration you should be able to, 1. Describe events in the respiratory processes 2. Discuss the mechanism of lung ventilation in human 3. Discuss the
IMPC phenotyping SOPs in JMC Indirect Calorimetry IMPC_CAL_002 Purpose Indirect calorimetry provides detailed information on the energy metabolism of mutant mice. Energy expenditure is evaluated through
Exploratory Activity: Line of Best Fit Revisited 1. Use the link http://illuminations.nctm.org/activity.aspx?id=4186 to explore how the line of best fit changes depending on your data set. A. Enter any
Page 1 of 19 : SOP of INNOVA 1412 Photoacoustic Multi-Gas Monitor Description and Principle of Operation The photoacoustic multi-gas monitor (INNOVA 1412, Innova AirTech Instruments, Denmark) is a highly
STANDARD OPERATING PROCEDURES Andersen Instruments Federal Reference Method (FRM) Ambient Particulate (PM 10 /PM 2.5 ) Sampler (RAAS) AMBIENT AIR MONITORING PROGRAM for the 130 LIBERTY STREET DECONSTRUCTION
Transpiration DataQuest 13 Water is transported in plants, from the roots to the leaves, following a decreasing water potential gradient. Transpiration, or loss of water from the leaves, helps to create
Video recording setup! 4 video cameras ( 2- underwater, 2- above)! Special trolley for moving 3 cameras along poolside! Start signal flash equipment ( under and above water)! 2 persons running all testing
ØRTS HANSEN, GRØNSEDT, LINDSTRØM GRAVERSEN AND HENDRIKSEN GUIDE TO THE ARCTIC SHIPPING CALCULATION TOOL THE NORTHERN SEA ROUTE VS. THE SUEZ CANAL ROUTE CBS MARITIME CROSS DISCIPLINARY PROBLEM-FOCUSED RESEARCH
Bluetooth is a communication technology that makes it possible to send and receive data without using wires. Using the Bluetooth features, you can set up a wireless connection between your NXT and other
Interdependence of Plants and Animals Computer 14 Plants and animals share many of the same chemicals throughout their lives. In most ecosystems, O 2, CO 2, water, food and nutrients are exchanged between
lorer /Eppendorf N) t Xplorer plus Register your instrument! www.eppendorf.com/myeppendorf Eppendorf Xplorer / Eppendorf Xplorer plus Adjustment Copyright 2015 Eppendorf AG, Germany. All rights reserved,
How to use the scrubber monitoring system on the rms Version 0.1 22 02 2012 not final yet: updates will follow Latest modifications in yellow Unfinished parts in italic 1 preparation of the unit Besides
Ventilator Training Module LTV (Lap Top Ventilator) Teaching Script LTV User guides: http://www.carefusion.com/documents/guides/user-guides/rc_ltv-1000_ug_en.pdf http://www.carefusion.com/documents/guides/user-guides/rc_ltv-1100_ug_en.pdf
Variable Temperature Operation AVIII 400 MHz Spectrometer November 11, 2012 The CBC NMR Laboratory University of Delaware AVIII 400 User Guide: Variable Temperature Operation 1 INTRODUCTION... 5 1.1 SAFETY...
GEM 2000 GAS ANALYZER & EXTRACTION MONITOR OPERATION MANUAL Copyright 2003 by CES-LANDTEC All rights reserved. Printed in the United States of America. No part of this book may be used or reproduced in
2018 The Race Director MyLaps Integration Manual [MYLAPS INTEGRATION] This document explains how to manage the results data between your MyLaps readers and Race Director using manual file transfers. Contents
Gas and Air Sensors User Manual asense VAV CO / temperature sensor with built-in general purpose controller General The IAQ-sensor product asense VAV is used to measure indoor air carbon dioxide concentration
D Alcotest 6510 Breath Alcohol Screening Device Operator Instructions for Use ST-207-2004_sw.eps Contents Contents................................................. 2 For your safety............................................
Virtual Pool 4 Made Easy Learn How to Play Virtual Pool 4 In Easy Steps Complete Menu Screen Guide Table of Contents QUICK PLAY QUICKSTART...1 GETTING STARTED...1 OVERVIEW...1 QUICK PLAY...2 INTRODUCTION...2
Technical Note ISE-730 and DO2-100 Overview In 1954, Dr. Leland Clark invented the first membrane-covered electrode designed to measure the concentration of oxygen in blood, or solution. This electrode
1 Experiment B-3 Respiration Objectives To study the diffusion process of oxygen and carbon dioxide between the alveoli and pulmonary capillaries. To determine the percentage of oxygen in exhaled air while
1 Experiment B-43 Rates Objectives To learn about the lungs and respiration s. To measure respiration s of the students. To compare female and male respiration s. To compare respiration s before and after