User Manual 2. Licensed under Rainin U.S. Patents 4,905,521 and 5,187,990 and corresponding foreign patents.

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1 ac Elecronic Pipeor Uer Manual 2 Manual RevD 1993, 1996, 1998, 2006 Marix Technologie Corp. All righ reerved. No par of hi publicaion may be reproduced, ored in a rerieval yem, or ranmied, in any form or by any mean, in whole or in par, wihou he prior wrien permiion of Marix Technologie Corporaion. Licened under Rainin U.S. Paen 4,905,521 and 5,187,990 and correponding foreign paen. Impac, TallTip, ECOTIPS, Marix Technologie Corp, and he Marix logo are regiered rademark of Marix Technologie Corporaion. Prined in he USA.

2 CONTENTS Congraulaion!... 1 Before You Begin... 1 A. INTRODUCTION... 2 A.1 Single Channel...3 A.2 Mulichannel...3 B. PIPETTOR OVERVIEW... 4 B.1 Componen...4 B.2 General Decripion...5 B.3 Keypad...6 B.3.1 Operaion Key...6 B.3.2 Programming Key...7 B.3.3 Special Funcion Key...8 B.4 Diplay...10 B.5 Pipeor Specificaion...11 B.6 Hazard and Precauion...12 C. PROGRAMMING AND OPERATION C.1 Programming he...13 C.1.1 Open Programming Mode or Scrach Pad (P-0)...13 C.1.2 Enering a Program in Open Programming Mode...13 C.1.3 Soring a Program...16 C.1.4 Program Recall...17 C.1.5 Adjuing Pipeing Speed...17 C.1.6 Reviewing he Program...17 C.2 Programming Example...18 C.2.1 Sample Tranfer...18 C.2.2 Incremenal Pipeing...19 C.2.3 Serial Diluion...19 C.2.4 Simple Diluion...20 C.2.5 Supernaan Collecion...20 C.3 Operaing Procedure...21 D. CALIBRATION D.1 ume Meauremen...23 D.2 Pipeor Calibraion...24 D.2.1 Facory Calibraion Sep...24 D.2.2 EASY Cal Mode...24 D.2.3 CALC Mode...25 D.2.4 CALVIEW...27 D.2.5 CALRESET...27 D.3 Recommendaion for Accurae Pipeing...27 E. TROUBLESHOOTING F. MAINTENANCE F.1 Single Channel Mainenance...32 F.1.1 Single Channel Diaembly...32 F.1.2 Single Channel Cleaning and Auoclaving...34 F.1.3 Pipeor Reaembly...34 F.2 Mulichannel Mainenance...35 F.2.1 Mulichannel Diaembly...35 F.2.2 Mulichannel Cleaning and Auoclaving...38 F.2.3 Mulichannel Mainenance and Reaembly...38 F.3 Inalling/Replacing Baerie...44 F.3.1 Inalling/Replacing NiMH Baerie...44 F.3.2 Inalling/Replacing Lihium-Ion Baery (16 Ch)...48 F.4 Charging/Recharging Baerie...50 F.4.1 Charging/Recharging NiMH Baerie...50 F.4.2 Charging/Recharging Lihium-Ion Baery (16 Ch)...51 G. TECHNICAL SERVICE G.1 Inrumen Warrany...53 G.2 On Sie Service - Calibraion & Mainenance...54 G.3 Facory Service - Calibraion, Mainenance, & Repair...55 G.4 Recommended Equipmen Deconamiaion Mehod...56 G.5 Equipmen Deconaminaion Form...57 ii iii

3 Congraulaion! You have purchaed he ae-of-he-ar in preciion pipeing. The i he fir cordle elecronic pipeor ha underand he myriad of liquid handling proocol ypically performed in oday laboraory. Ergonomically deigned and balanced o reduce faigue, he funcion o inuiively, you may need no more han a quick urvey of hi manual o ue i. The execue your liquid pipeing ak quickly, accuraely, and wih le rik of developing repeiive rain injurie (RSI) ypically aociaed wih manual pipeor ue. Laboraory reul will become more andardized, while le ime i expended on ediou bench procedure. Before You Begin Before uing he, verify he conen of he package. The package hould conain he following iem: 1 Pipeor 1 Operaor Manual 2 Rechargeable NiMH Baerie or 1 Lihium-Ion Baery (16 Channel) 1 AC Power Tranformer 1 Warrany Card 1 Accuracy/Preciion Daa Shee If iem are miing, conac a Marix repreenaive or conac cuomer ervice a (800) Noe: Baerie mu be inalled and recharged before uing he Refer o he following ecion in hi manual: Secion F.3, Inalling/Replacing Baerie Secion F.4, Charging/Recharging Baerie 1

4 A. INTRODUCTION A.1 Single Channel Inroducion I ime o Impac your lab! The i he econd generaion of he Impac pipeor family, providing he convenience of a manual pipeor, wih he programming capabiliie of an elecronic pipeor. The pipeor feaure include: Expanded memory for oring up o five pipeing program. Each program can hold a maximum of 40 ep. Scrach Pad programming mode which allow for fa and imple program change ha are no ored ino memory. Enhanced peed conrol offer lower apiraing and dipening of vicou liquid. Excellen for gel loading! EASY CAL which implifie in-lab calibraion verificaion uing diilled waer. The i lighweigh and cordle, uilizing rechargeable baerie, which can be ued while recharging (wih he excepion of ingle channel pipeor). The pipeor alo ha an auomaic hu-off feaure o exend baery life. The ue inegraed circui echnology o ore programming informaion. I will hold up o 40 ep in a program, wih up o 5 program aved in memory, and 1 crach pad (See ecion C.1.1 Open Programming Mode or Scrach Pad ). The program informaion i reained in memory, even if he baerie are fully dicharged or removed. The ue a paced dipene feaure ha perform repea pipeing ep while he rigger i depreed. The ingle channel pipeor are available in a number of model configuraion (ee Figure A-1) from 12.5µl o 1250µl, ranging in volume from 0.5µl o 1250µl. Each pipeor i color-coded wih a color ring for eay volume idenificaion (ee ecion B.5, Pipeor Specificaion). Two model, 250µl and 1250µl, are alo available a a Shory, which are horer in overall lengh compared o he andard pipeor. 1250µl 250µl 125µl 30µl 12.5µl Figure A.1: Single Channel Model A.2 Mulichannel 1250µl Shory 250µl Shory The mulichannel pipeor are available in a number of configuraion from 8 o 16 channnel. An expandable EXP i alo available in 6 channel and cuom configuraion. Model range in volume from 0.5µl o 1250µl. Each pipeor i color-coded wih a color ring for eay volume idenificaion (ee ecion B.5, Pipeor Specificaion). Pleae ee our Equalizer for a pipeor ha ha equal ip pacing beween 4.5mm and 14.15mm (depending on model). 6-Channel Expandable 8-Channel 12-Channel 16-Channel 384 Model Figure A.2: Mulichannel Model 2 3

5 Pipeor Overview B. PIPETTOR OVERVIEW B.1 Componen B.2 General Decripion Keypad. The keypad conain he operaion key ha are ued in programming he pipeing equence and volume. (See Secion B.3, Keypad). Pipeor Overview Diplay. The diplay i an LCD creen ha how he pipeing operaion (fill, dipene, mix, or purge) and fluid volume for each ep in he pipeing program. (See Secion B.4, Diplay). Baery Cae. The baery cae conain he rechargeable baerie. Nickel Meal Hydride baerie fully charge in hour, while Lihium-Ion baerie fully charge in 2 hour. The can perform a full day workload wih no need o recharge. Baery life i exended wih auomaic hu-off afer 10 minue of inaciviy. (See Secion F.3, Inalling/Replacing Baerie). Pipeing Trigger. The pipeing rigger iniiae a program. A ingle pipeing ep i execued by a quick pre and releae of he rigger. Repea pipeing i execued by preing and holding he rigger. Noe: A he end of any dipene, he pipe ip hould be removed from he fluid before releaing he rigger, o preven apiraion of fluid. Figure B.1a: Single Channel Componen Ejecor Trigger. The ejecor rigger i preed o ejec he pipe ip from he pipeor. Tip Fiing. The ip fiing form an airigh eal wih he pipe ip o enure accuracy while pipeing. Noe: pipeor are equipped wih chemically-reian (PEEK) ip fiing (and ejecor leeve on ingle channel model) allowing pipeing of acidic oluion wihou damage o he ip fiing. Color-coded Ring. The ring around he pipeor handle i color-coded o idenify he pipeing volume of he handle (See Secion B.5, Pipeor Specificaion). Tranformer Connecor. The ranformer connecor receive he plug from he power ranformer for recharging he baerie. The pipeor can be ued while recharging (wih he excepion of ingle channel pipeor). Figure B.1b: Mulichannel Componen 4 5

6 Pipeor Overview Pipeor Overview B.3 Keypad The keypad conain he operaion key, programming key, and he pecial funcion key. [Purge] The Purge key ener a purge promp anyime during a pipeing operaion o abor he program. By preing he Purge key, hen preing he rigger, you can dipene any remaining liquid from he pipe ip and reurn o he beginning of he program. The Purge key i alo ued o ener he purge ep ino a program. Noe: If here i inufficien liquid for a final dipene ep, he pipeor emi a beep one and auomaically diplay he purge promp. Figure B.3: Keypad RUN The RUN mode perform he operaion ha i diplayed, uch a fill, dipene, mix, or purge. When Run appear in he diplay, you can acivae he RUN mode by preing he rigger. A he end of a pipeing equence, he pipeor emi a riple beep one and reurn o he beginning of he program. The pipeor remain in RUN mode unil you program a new pipeing equence. B.3.1 Operaion Key B.3.2 Programming Key [On/Off] [Prog] The On/Off key urn he pipeor on or off. When he pipeor i urned on, i emi a beep one, flahe ZERO on he diplay, hen auomaically ree he pion o zero poiion. Afer zeroing, he diplay how he fir ep of he program in he RUN mode. Noe: If he pipeor i being programmed for he fir ime, he fir ep will be he fill operaion, wih he defaul fill volume. (See Secion C.1, Programming he, for he defaul eing). The Program key on he enable you o ore up o five eparae program in memory for fuure recall. Each program can hold up o 40 ep. Program are ored in reerved program locaion number 1 5. Program 0 i ued a a crach pad where emporary program can be wrien, run, and overwrien eaily. Program 0 i conidered o be an open program. (See Secion C.1, Programming he ). [Fill] [Dip] [Mix] The Fill, Dipene, and Mix key program he pipeing operaion. By preing one of hee key, you can ener he programming mode of he. The Run promp diappear from he diplay and he appropriae operaion appear wih a flahing promp. Fill, dipene, or mix volume can be programmed by preing he ume key. Alo, he number of mix cycle can be programmed by preing he Mix key wice. Auomaic blowou: A he end of a final dipene ep, he pipeor auomaically expel any remaining liquid from he pipe ip wih a hor bur of air (blowou). The pion hen revere direcion and reurn o zero poiion. 6 7

7 Pipeor Overview Pipeor Overview Noe: If you pre and hold in he rigger during he final dipene ep, he pipeor perform he auomaic blowou and hold i poiion unil you releae he rigger. Thi delay allow you ufficien ime o remove he ip from he liquid o preven apiraion of liquid, a he pion reurn o zero poiion. The ume key conrol he quaniy of liquid o be apiraed or dipened. The volume can be enered when he promp appear. Pipeing volume can be incremened quickly by coninuouly depreing he ume key. The longer you hold he key, he greaer he incremenal peed. The ume key can alo be ued o change pipeing peed wihin a ored program or during he execuion of a program. (See Secion C.1.5, Adjuing Pipeing Speed). Double beep one: operaion or programming key i no acive in curren mode; inufficien dipene volume, purge required; or illegal programming ep aemped (ee Secion E, Troublehooing). Triple beep one: end of pipeing equence; end of programming equence. There are hree opion o chooe from in eing he beep one. ON1 will have all one ound a he appropriae ime lied above. ON2 will ue only double and riple beep one. OFF will urn off all one excep for he one o ignify an illegal programming ep. Pre he Special Funcion key wice o diplay TONE and i curren eing. To change he eing, pre he ume key. If he beep one i e for ON1 or ON2, he beep one ymbol appear a he boom of he diplay. Noe: For paced dipening, he beep one hould be e ON1, o prepare you for each dipene ep. [Ener] The Ener key ore he program in memory. Afer each ep i programmed, he Ener key i preed once o accep he ep. Afer he final ep i programmed, he Ener key i preed wice o denoe he end of he program. The pipeor verifie he end of he program wih a riple beep one. B.3.3 Special Funcion Key The Special Funcion key i he mall hole below he ume key. I can be acivaed wih a pipe ip. By repeaedly preing he Special Funcion key, you can diplay four funcion: Pipeing Speed (SPd), Beep Tone (TONE), Paced Dipene Speed (PACE), and Calibraion (CAL). To exi he Special Funcion key a any ime, pre he Ener key. TONE The beep one ignal he compleion of one or more ep, or ignal a warning: Single beep one: end of pipeing ep. PACE CAL The pace conrol he peed of repea pipeing (paced dipening), FAST, SLOW or OFF. Pre he Special Funcion key hree ime o diplay PACE and i curren eing. To change he eing, pre he ume key. Paced Dipene. When uing he paced dipene feaure, he pipeor dipene he programmed volume a he pecified pace (fa or low). Immediaely afer each dipene ep, he pipeor emi a ingle beep one (if beep one i on) o prepare you for he nex dipene ep (ee Secion C.5, Operaing Procedure). The calibraion of he pipeor i facory e for diilled waer a room emperaure. If you are pipeing fluid wih ignificanly differen pecific graviie or emperaure, ue hi eing o recalibrae he pipeor. (See Secion D, Calibraion). 8 9

8 Pipeor Overview Pipeor Overview SPd Once in he pecial funcion mode, adju he pipeing peed by preing he ume key. Five peed eing are available: B.5 Pipeor Specificaion Speed Seing Slow S Medium Slow S z Diplay Medium S z z Medium Fa S z z z Fa S z z z F The curren peed eing appear a he boom of he diplay. Thee eing can be eleced and changed a any ime before a program ep i execued. B.4 Diplay The pipeor diplay i a dynamic LCD creen. I diplay he curren operaion, volume promp (if enering a program), pipeing volume, low baery indicaor (appear if baery charge i low), beep one ymbol (if he beep one i on), pipeing peed, and program number. Iem No. ume Channel ume Range Color Ring Accuracy* (±) Preciion** (±) µl Single Ch µl Red 1.0%/0.05µl 0.4%/0.05µl µl Single Ch µl Purple 1.0%/0.15µl 0.3%/0.05µl µl Single Ch µl Yellow 0.6%/0.3µl 0.2%/0.1µl µl Single Ch 5-250µl Blue 0.6%/0.5µl 0.15%/0.15µl µl Single Ch µl Green 0.5%/3.0µl 0.13%/0.6µl µl Shory 5-250µl Blue 0.6%/0.5µl 0.15%/0.15µl µl Shory µl Green 0.5%/3.0µl 0.13%/0.6µl µl 6-Ch EXP µl Yellow 2.0%/1.0µl 1.0%/0.6µl µl 6-Ch EXP µl Blue 2.0%/1.5µl 0.7%/1.0µl µl 6-Ch EXP µl Green 1.5%/6.0µl 0.6%/3.0µl µl 8-Ch µl Red 2.5%/0.15µl 2.0%/0.15µl µl 8-Ch µl Yellow 2.0%/1.0µl 1.0%/0.6µl µl 8-Ch µl Blue 2.0%/1.5µl 0.7%/1.0µl µl 8-Ch µl Green 1.5%/6.0µl 0.6%/3.0µl µl 12-Ch µl Red 2.5%/0.15µl 2.0%/0.15µl µl 12-Ch µl Yellow 2.0%/1.0µl 1.2%/0.8µl µl 12-Ch µl Blue 2.0%/1.5µl 0.8%/1.2µl µl 12-Ch µl Orange 1.5%/4.0µl 0.6%/2.5µl µl 16-Ch µl Red 2.5%/0.15µl 2.0%/0.15µl µl 16-Ch µl Purple 2.0%/0.30µl 1.7%/0.30µl µl 16-Ch µl Yellow 2.0%/1.0µl 1.2%/0.8µl Table B.5 Pipeor Specificaion Noe: For Accuracy and Preciion, value hown are expreed a a percen (%) deviaion or microlier (µl) value. When applied o deired volume, he greaer of he wo value will alway apply. Figure B.4: Diplay * Boh value repreen he deviaion from he mean. ** Percenage value are expreed a he coefficien of variaion. Microlier value are expreed a he andard deviaion

9 Pipeor Overview B.6 Hazard and Precauion There are no known hazard aociaed wih he when i i operaed in accordance wih he inrucion in hi manual. However, you hould be aware of iuaion ha can reul in eriou injury. Noe: Do no perform roublehooing procedure on he inernal componen unle inruced by Marix Technical Service peronnel. WARNING! Enure ha he power ranformer i conneced o a power recepacle ha provide volage and curren pecified by Marix. Ue of an incompaible power recepacle can caue hock and fire hazard. CAUTION! Ue only he power ranformer upplied by Marix Technologie. Ue of an incompaible power ranformer can damage he pipeor. CAUTION! Alway urn off he power and unplug he power ranformer before cleaning he exerior. Fluid eepage can damage inernal componen. CAUTION! Do no immere he uni in liquid. Fluid eepage can damage inernal componen. CAUTION! Do no auoclave he enire uni. Exreme hea can damage he diplay and oher elecronic componen. Noe: For auoclaving pecific porion of he pipeor, refer o he Auoclaving Procedure, or call Marix Technical Service for inrucion. CAUTION! Do no clean he keypad wih bleach oluion or oher olven. Cauic cleaning oluion can damage he keypad. CAUTION! Avoid exceive charging of he baerie when he pipeor i no in ue. Exceive charge o he baerie will horen he baery life and may damage he baerie. C. PROGRAMMING AND OPERATION C.1 Programming he Programming and Operaion Programming he i fa and eay. Pipeing ep and volume are enered in he equence ha hey will be performed, hen he program i aved o memory. Thi program will ay in memory (even if he pipeor i urned off, or he baery run low) unil you change i. Five program and one crach pad (Program 0), each capable of holding 40 ep, can be enered and ored in he. C.1.1 Open Programming Mode or Scrach Pad (Program 0) The provide ample program orage for five, 40 ep program. In addiion, i offer open programming mode, alo known a he crach pad. Thi mode i idenified by P-0, which appear in he program ecion of he diplay (refer o he diplay on page 10). Thi feaure enable quick change o pipeing proocol wihou affecing previouly ored program. To ue he crach pad mode: From he ar of any program, imply begin enering a new proocol (elecing a pipeing operaion a decribed below). Thi new proocol can now be ued, and will remain acive unil a ored program i rerieved. P-0 appear in he program ID ecion of he diplay o indicae ha he (O)pen programming mode, or crach pad mode, i acive. C.1.2 Enering a Program in Open Programming Mode To ener a pipeing program: 1. Turn on pipeor. Pre he green [On/Off] key o urn on he pipeor. The pipeor will beep once and flah ZERO on he diplay while reeing he pion o zero poiion. The fir ep of he curren program i diplayed. Noe: If he pipeor i being programmed for he fir ime, he fir ep i he fill operaion, wih he defaul fill volume diplayed (ee he following able)

10 Programming and Operaion Programming and Operaion ume Fill ume Dipene ume Mix ume Table C.1.2: Defaul Programming ume (in µl) 2. Selec pipeing operaion. Ener he programming mode by preing he [Fill] or [Mix] key. For example, o begin programming wih a filling operaion, pre he [Fill] key. The diplay how Fill wih a flahing promp: Figure C.1.2a: Flahing ume Diplay Noe: The diplayed volume defaul o he la volume programmed for he eleced operaion, or o he defaul volume for he pipeor, if i i being programmed for he fir ime. Fill. The [Fill] key program he pipeor o apirae a volume of liquid. A ingle fill ep can be programmed for fluid ranfer, or a combinaion of fill ep can be programmed for diluion or upernaan collecion. Dipene. The [Dip] key program he pipeor o dipene a volume of liquid. The dipene volume can be he enire fill volume, or a maller incremen for repea pipeing. The dipene volume need o be programmed only once for repea pipeing. The pipeor will dipene he volume each ime he rigger i preed (or repeaedly during a paced dipene) unil he fill volume i reached, or unil inufficien volume remain. Mix. The [Mix] key program he pipeor o perform a combinaion of fill and dipene. You can program a mix volume or mix cycle (ingle fill and dipene). You can pre [Mix] once o program a mix volume, or wice o program a mix cycle. Mix cycle from 2 o 9 are available on he. The defaul eing i hree mix cycle. Some programming conideraion for he mix operaion: If he program begin a mix cycle from zero poiion, i will complee he cycle wih an auomaic blowou, hen diplay ZERO. Pre he rigger o reurn he pion o zero poiion. If he program begin a mix cycle above zero poiion (volume i greaer han zero), he fluid i dipened, and he mix cycle begin. The mix cycle will end a he ame volume i ared wih. Alo, he mix operaion will remain in he diplay o i can be repeaed (ueful for erial diluion), unle followed by anoher program ep. To end he mix operaion, pre he [Purge] key, hen pre he rigger. Purge. The [Purge] key program he pipeor o purge he fluid. Thi ep can be enered a a program ep, or i can be preed any ime while pipeing o erminae he program and dipene he remaining liquid. Noe: If he program end a a volume greaer han zero, and he la ep i no a mix cycle, he Purge promp auomaically appear. Alo, he pipeor emi a double beep one o warn you ha ome fluid remain. Pre he rigger o dipene he remaining fluid and reurn o he beginning of he program. Example of pipeing program wih differen mix ep are hown in Secion C.2, Programming Example. 3. Ener volume. Ener he deired volume for [Fill], [Dip], or [Mix] by preing he up or down ecion of he [] key. The longer you hold he key, he greaer he incremenal peed. Noe: The [Purge] key doe no ue a volume; herefore, if a purge ep ha been enered, coninue o he nex ep

11 Programming and Operaion 4. Confirm programming ep. Pre [Ener] o confirm he programming ep. The diplay flahe all of he operaion key o promp he nex programming ep: Programming and Operaion The program number will be diplayed for wo econd. (The fir ep of he newly wrien program i diplayed). Thi program i ready o be run. C.1.4 Program Recall To recall a ored program: 1. Pre he [Prog] key, hen he [] key o elec he deired program number. 5. Selec nex pipeing operaion. Repea ep 2 o 4 unil all programming ep have been enered. 6. Save program and exi. When he diplay flahe all operaion key, pre [Ener] o ave he program and exi he programming mode. The pipeor emi a riple beep one o ignal he end of he programming equence. The fir ep in he program i diplayed. Run i diplayed in he lower lef corner o indicae ha he new program i ready o be run. Go o Secion C.1.6 for Reviewing he Program, or Secion C.3 for Operaing Procedure. C.1.3 Soring a Program Figure C.1.2b: Flahing Diplay To creae and ore program in he : 1. Selec he program number by preing he [Prog] key, hen pre he [] key unil he deired program number (P1-P5) i diplayed. 2. Pre and hold he [Prog] key, while imulaneouly preing he Special Funcion key o ener he programming mode. The promp Prog will appear on he diplay. 3. Begin enering your new program (refer o Secion C.1.2, Enering a Program in Open Programming Mode, for inrucion). NOTE: When programming mode i acive, he Speed eing and Beep Tone ymbol will blink. 2. Pre he [Ener] key o acivae he diplayed program. C.1.5 Adjuing Pipeing Speed The offer unique peed conrol programming for Fill, Dipene, Mix, or Purge. Speed wihin any of he five ored program can be independenly e and ored a an inegral par of he pipeing proocol. Pipeing peed i eaily changed a he ar of each pipeing funcion. To change pipeing peed during he execuion of a program: Pre he [] key o elec he peed for each pipeing ep (Fill, Dipene, or Mix only). Noe: Purge mu be changed in he program. Thi newly e peed will remain acive wih each funcion even if he pipeor i urned off. Speed eing wihin any of he five ored program will alo remain in memory wih he program funcion unil changed by he operaor. C.1.6 Reviewing he Program Afer programming he pipeor, review he programming ep: 1. Se pipeor for RUN mode. Enure he pipeor i in he RUN mode ( RUN appear in he lower lef corner of he diplay). If he diplay i flahing all pipeing operaion, i i ill in he programming mode; pre [Ener] o ave he program and exi. 2. Begin program. Run he program wihou uing pipe ip or apiraing fluid. Pre he rigger for each ep in he program. Upon preing he [Ener] key o ave and exi he program, he new program will be ored under he eleced program number

12 Programming and Operaion Programming and Operaion 3. Oberve diplay. A each ep of he program, oberve he diplay. If neceary, re-ener he programming equence. Some helpful hin for reviewing your program: If he program i complex and conain everal ep, wrie he ep on paper before enering he program. Check off each ep a you review he program. If he program i inended for paced dipening, review he program again, wih he rigger depreed unil he la ep. Check he dipene pace o enure he dipene ep are a comforable peed. C.2 Programming Example Example of differen pipeing equence are decribed below, and are ideal for ue a raining exercie. Pre he key hown in bracke [ ], hen pre he [] key unil he volume in parenhee ( ) i diplayed. A he end of he program, a riple beep one i ounded. (ee ecion D.3 Recommendaion for Accurae Pipeing for uggeion for beer preciion and accuracy). C.2.1 Sample Tranfer Example: Fill and dipene 100µl. [Fill] Opion 1 Opion 2 (100) [Ener] or [Fill] (100) [Ener] [Ener] C.2.2 Incremenal Pipeing Example: Fill and dipene 250µl. [Fill] [Dip] [Dip] (250) [Ener] (10) [Ener] (45) [Ener] [Ener] In hi program, only one dipene ep need o be programmed. If any program end in a dipene ep, he pipeor repea he dipene ep unil he volume i zero, or i le han he programmed dipene volume. If he remaining volume i le han he programmed dipene volume, he pipeor diplay a Purge promp o dicard he remainder. Thi dicarded volume conain he cumulaive error of previou pipeing (ee ecion D.3 Recommendaion for Accurae Pipeing for furher explanaion). C.2.3 Serial Diluion Noe: The 10µl Primer Dipene reduce he percenage of error in incremenal dipening (ee ecion D.3 Recommendaion for Accurae Pipeing) Example: Tranfer 100µl o he fir column of a plae previouly filled wih reagen. Mix 200µl for 2 cycle. Tranfer 100µl o he econd column and repea he mix ep. Follow hi procedure for he re of he plae. [Dip] (100) [Ener] [Ener] [Fill] (100) [Ener] The econd opion i a one-ep program. Becaue he program end a a volume greaer han zero, and he la ep i no a mix, a Purge promp auomaically appear a he end of he fill o dipene he liquid and reurn o he beginning of he program. [Mix] (200) [Mix] (2) [Ener] [Ener] [Ener] Tha he enire program! The mix ep alway reurn o he volume a which i ared (100µl); herefore, he pipeor coninue he mix ep indefiniely, unil you pre he [Purge] key o end he program. In hi example, he mix cycle i programmed for 2 cycle (defaul i 3 cycle)

13 Programming and Operaion C.2.4 Simple Diluion Example: Prepare 1:10 diluion wih a 10µl ample volume. [Fill] [Fill] [Fill] (100) [Ener] (20) [Ener] (10) [Ener] [Purge] [Ener] [Ener] C.3 Operaing Procedure Programming and Operaion Afer programming he pipeor, he diplay hould how he fir program ep, wih he Run promp howing in he lower lef corner: Figure C.3: Run Promp The following ep decribe a baic program for pipeing reagen ino a microier plae. The pipeor i programmed a follow: The fir fill volume i for he diluen (100µl), followed by an air gap (20µl), and finally by he ample volume (10µl). The purge ep will dipene he oal volume. C.2.5 Supernaan Collecion Example: Apirae 100µl from 8 row of a microier plae and dicard he oal volume. [Fill] (100) [Ener] [Fill] [Ener] [Fill] [Ener] [Fill] [Ener] [Fill] [Ener] [Fill] [Ener] [Fill] [Ener] [Fill] [Ener] [Purge] [Ener] [Ener] Thi program i performed wih he 850µl handle. All 100µl fill volume are colleced in he ame ip. When compleed, he oal volume i dicarded wih he purge ep. [Fill] [Dip] [Dip] (250) [Ener] (10) [Ener] (30) [Ener] [Ener] Noe: The 10µl Primer Dipene reduce he percenage of error in incremenal dipening (ee ecion D.3 Recommendaion for Accurae Pipeing) 1. Aach pipe ip. When ready o begin pipeing, aach he pipe ip o he pipeor. Noe: When applying ip, avoid banging or applying exceive force o he ip(), a hi reche he ip eal and will affec volume. Ue Marix ip which have been pecifically deigned o mach he ip fiing on Marix pipeor. 2. Apirae liquid. Immere he pipe ip ino he liquid o be apiraed, hen pre he rigger o apirae 250µl. 3. Dipene primer volume. Dicard he 10µl primer volume. 4. Dipene liquid. Poiion he pipe ip over he fir column of well in he microier plae, and pre he rigger o dipene 30µl. A he compleion of he dipene ep, he pipeor emi a ingle beep one (if he beep one i e o ON1)

14 Programming and Operaion 5. Repea dipene. Repea he previou ep for he re of he column in he microier plae. A he compleion of he eighh dipene, he pipeor emi a double beep one (if he beep one i on, oherwie i emi a ingle beep one) and diplay he Purge promp. There i inufficien volume for anoher 30µl dipene, and he pipeor ignal for a purge ep. -or- Paced dipene. Sep 4 and 5 can alo be performed a a paced dipene. Inead of preing he rigger for each dipene ep, pre and hold he rigger o auomaically dipene each volume. Immediaely afer each dipene ep, he pipeor emi a ingle beep one (if he beep one i on), promping you o prepare for he nex dipene ep. To op a paced dipene, releae he rigger before he end of he la dipene ep. Noe: For paced dipening of mall volume, e he pace o SLOW. Enure ha you have fully releaed he rigger before he end of he la dipene ep o preven an addiional, unwaned dipene ep. 6. Dicard remaining liquid. Poiion he pipe ip over a wae conainer and pre he rigger o purge he remaining liquid. The pipeor emi a riple beep one o ignal he end of he program, hen reurn o he fir ep of he program. 7. Ejec ip. Pre he ejecor rigger o ejec he ued pipe ip. D. CALIBRATION Calibraion The i facory-calibraed for diilled waer a 20 C. For eay calibraion uing diilled waer, he pipeor feaure an EASY CAL mode (ee Secion D.2.2, EASY CAL mode). When pipeing liquid of ignificanly differen pecific graviie or emperaure, however, he Calibraion (CALC) feaure may be ued o achieve greaer accuracy (ee Secion D.2.3, CALC mode). To prepare for calibraion, enure he pipeor and ip are equilibraed. When calibraing a mulichannel pipeor, elec only one channel o pipe wih. Uing an analyical balance, fir obain he acual value of he programmed volume a facory calibraion. The programmed volume (Vp) ued i ypically he full volume of he pipeor (for example, 200µl for he 250µl 8-channel pipeor), bu i no limied o hi volume. D.1 ume Meauremen To meaure he acual value of he programmed volume: 1. Fill he programmed volume (Vp), hen dipene he enire volume ino a conainer on he analyical balance. 2. Read he weigh on he analyical balance. Repea hi procedure en ime. Noe: Ue a clean and dry pipe ip wih each pipeing cycle. When filling he liquid, immere he pipe ip approximaely 1/4 inch (approx. 0.6 cm) below he liquid urface. When dipening he liquid, ouch he ide of he conainer o enure a complee dipene. 3. Deermine he average weigh of he programmed volume and conver i o volume. To conver o volume, correc he weigh for pecific graviy and emperaure. If weighing waer, ue one of he correcion facor below: Temperaure Correcion Facor 20 C C C - 25 C The reuling volume i he correced acual volume. If he pipeor i correcly calibraed, he programmed volume hould equal he acual volume (wihin he pecificaion of he pipeor)

15 Calibraion D.2 Pipeor Calibraion uer can calibrae heir pipeor wih EASY CAL mode for diilled waer or wih CALC mode o calibrae oher liquid and for emperaure oher han room emperaure. D.2.1 Facory Calibraion Sep The facory calibraion ep i needed when calibraing your. Thi i locaed on he calibraion cerificae called Accurary and Preciion Verificaion, encloed in he documenion for hi pipeor. (See iem CAL STEPS in he upper righ hand box). View he pipeor curren calibraion ep by uing he CALVIEW mode lied in ecion D.2.4. Noe: When reeing he calibraion ep o a defaul value, a lied in ecion D.2.5 CALRESET, he ep i e o an average value for all pipeor of i cla. Thi value may differ from he facory-opimized calibraion ep documened on he Accuracy and Preciion Verificaion cerificae. Pleae ue he ep lied here for be reul. If you canno locae you documenaion ha li he facory calibraion ep, conac Marix Tech Suppor a , and have your pipeor erial number ready. D.2.2 EASY Cal Mode uer can quickly calibrae heir pipeor by uing he unique EASY CAL feaure. EASY CAL ha been developed for fa, eay, in-lab verificaion and/or calibraion uing diilled waer a room emperaure ( C). ume Calibraion Poin (µl) Facory Calibraion Sep 12.50µl 10.00µl Locaed on he calibraion 30µl 25.0µl cerificae Accuracy and Preciion Verificaion 125.0µl 100µl hee included in he 250.0µl 200µl pipeor documenaion. 850µl 700µl See iem CAL STEPS in 1250µl 1000µl upper righ hand box. Table D.2.2: Calibraion Specificaion Calibraion 1. Weigh ou en aliquo of diilled waer on an analyical balance, uing one pipeing channel. The programmed dipene volume mu correpond o he calibraion poin for ha pipeor (for example, 200µl for a 250µl pipeor). Refer o Table D.2.2, Calibraion Specificaion, for proper calibraion poin. Noe: Enure ha he diilled waer ued i a room emperaure ( C). An inernal correcion facor of i ued by he pipeor o conver weigh (gm) o volume (ml). 2. Calculae an average for he en reading of diilled waer obained from he balance. If he reuling average weigh i wihin your accuracy pecificaion, no furher calibraion i required. If he average weigh doe no mee expeced performance, ener he EASY CAL mode o quickly re-calibrae he. 3. Ener he EASY CAL mode by preing he Special Funcion key (uing a pipe ip) unil EASY flahe, hen pre he [Ener] key o diplay he calibraion poin. 4. Ue he [] key o adju he diplayed calibraion poin unil i mache he average weigh obained in ep 2. Sore hi number by preing he [Ener] key. The new calibraion eing i confirmed by he CAL and SET meage on he diplay. 5. Verify hi new calibraion eing by repeaing Sep 1 and 2. D.2.3 CALC Mode To calibrae he pipeor: 1. Weigh ou en aliquo of diilled waer on an analyical balance, uing one pipeing channel. The programmed dipene volume mu correpond o he calibraion poin for ha pipeor (for example, 200µl for a 250µl pipeor). Refer o Table D.2.2, Calibraion Specificaion, for proper calibraion poin. Noe: Enure ha he diilled waer ued i a room emperaure ( C)

16 Calibraion Calibraion D.2.4 CALVIEW 2. Calculae diplay volume of he pipeor: Vp = Programmed ume Va = Correced Acual ume (correced for emperaure) Cp = Calibraion Poin (each pipeor handle ha a unique calibraion poin, ee able D.2.2: Calibraion Specificaion.) Dv = Diplay ume 3. Ener he CAL mode on he pipeor by preing he Special Funcion key (ue a pipe ip o pre he key) unil EASY appear on he diplay. 4. Pre he [] key o diplay CALC (preing he [] key again will reurn you o he EASY mode). 5. Pre he [Ener] key o diplay he calibraion poin. 6. Pre he [] key o ener he Diplay ume (Dv) calculaed above in ep Pre he [Ener] key o e he calibraion poin. The diplay how CAL hen SET o confirm he new calibraion poin, and reurn o he curren pipeing program. Example: Calibrae a 1250µl 8-channel pipeor. Waer emperaure = 24 C Programmed ume (Vp) = 1000µl Average of Acual ume = 990µl Correced Acual ume (Va) = 990 x = 994µl Calibraion Poin (Cp) = 1000 D V = V p V a x C p Dv = Vp Va x Cp CALVIEW diplay he pipeing roke (number of ep) ued o reach he calibraion poin. Thi value allow you o confirm he calibraion during pipeing and afer calibraion. To acivae CALVIEW, ener he CAL mode uing he Special Funcion key. When he calibraion poin i diplayed, pre he [Fill] key. The number of ep i diplayed. Pre any key o exi CALVIEW. D.2.5 CALRESET CALRESET allow you o reurn o a defaul calibraion ep. To ree he calibraion, ener he CAL mode uing he Special Funcion key. When he calibraion poin i diplayed, pre he [Purge] key. The diplay flahe FACT, CAL, hen SET and he pipeor ree o he defaul ep. Thi defaul eing i an average value for all pipeor of i cla. Thi value may differ from he facory-opimized calibraion ep documened on he Accuracy and Preciion Verificaion cerificae encloed in hi pipeor documenaion. (See ecion D.2.2, Calibraion Specificaion). D.3 Recommendaion for Accurae Pipeing pipeor are facory calibraed o deliver accurae volume wih diilled waer a 21 C and 50% humidiy. By uing he calibraion procedure decribed above, you can pipe liquid of differen pecific graviy wih a high degree of accuracy and reproducibiliy. For opimal pipeing reul, refer o he following recommendaion: Pipee a conan a emperaure. Allow liquid and equipmen o equilibrae o ambien emperaure. ume apiraion wih air diplacemen pipe varie wih air preure, relaive humidiy and he liquid vapor preure, all of which are emperaure dependen. Aach ip wih low even preure. When applying ip, avoid banging or applying exceive force o he ip(), a hi reche he ip eal and will affec volume. Ue Marix ip which have been pecifically deigned o mach he ip fiing on Marix pipeor. 1000µl D V = x µl D V =

17 Calibraion Calibrae he pipeor for he liquid. If he liquid ha a pecific graviy and vapor preure equivalen o diilled waer, calibrae uing EASY CAL mode. Oherwie, ue CALC mode. Pre-we he pipe ip. Apirae and dipene a few ime before apiraing he liquid o increae humidiy wihin he ip, reducing ample evaporaion ha reul in lower dipene volume in iniial few pipeing. The need o pre-we increae when working wih liquid wih low vapor preure and/or high urface enion. Immere ip fully. Tip hould be immered approximaely 1/4 inch (approx. 0.6 cm) below he liquid menicu and away of he conainer urface o avoid apiraion of air and exce drople clinging o he ouide of he ip. Dicard a primer volume. When he moor change from apiraion o dipene, he percenage of error i greaer in he fir dipene han in ubequen dipene. Paue conienly. I ake a momen for he liquid in he ip o finih moving. Paue wih he ip in he liquid for abou one econd afer apiraing he ample o allow he liquid o finih moviing ino he pipe ip afer he pipeor op. Oherwie, he volume will be lower han i hould. Apirae low, dipene fa. A a general rule, pipe liquid, epecially vicou, a a lower peed o enure accurae pipeing. Dipene a faer peed o expel liquid more accuraely. Noe: A he end of any dipene, remove pipe ip before releaing he rigger, o preven apiraion of fluid. Tip ouch. During he dipening of a liquid, ouch he ip again he ide of he receiving conainer o preven any liquid from remaining on he ip. Dicard final dipene. When accurae dipening of everal incremen of liquid are needed, dicard he la incremen. The cumulaive percenage of error from previou incremen exi on he la volume. (i.e. The pipeor apirae 100µl and dipene 20µl five ime, and if each of he fir four dipene were off by +1.0µl, hen he la dipene i miing 4µl before you even dipene i). E. TROUBLESHOOTING Troublehooing Poible procedural and yem problem, a well a recommended oluion. If you canno reolve he problem, call Marix Technologie Technical Service Deparmen a No power o he pipeor. Probable Caue Baerie are incorrecly inalled. Baery pack cable are no properly conneced. Baery Spacer i no in uni or i no in correcly (Mulichannel only wih NiMH). Double beep one Probable Caue occur. An illegal programming ep i being enered, uch a programming wo fill ha exceed he maximum fill volume. Recommended Soluion Recheck he orienaion of he baerie; hey hould mach he diagram in he baery cae Enure he connecion o he baery pack are ecurely plugged ino he connecion from he pipeor body. Enure ha he baery pacer i in place for all model ha ue Nickel Meal Hydride (ee ecion F.3.1). Recommended Soluion Recheck he programming equence. Enure you are no aemping o fill above he maximum fill volume of he pipeor, or aemping o dipene more han he oal fill volume. ERR_1 i diplayed. (Signifie one of he following condiion) Probable Caue Recommended Soluion Pipeor canno complee auomaic blowou. Baerie are low on power, cauing he moor o all. Liquid i apiraed ino he pipeor. Moor i defecive or he flex circui aembly i damaged. Recharge he baerie immediaely. (See Secion F.4, Charging/Recharging Baerie). Diaemble and clean he pipeor. (See Secion F.1 and/or F.2, for pipeor cleaning and auoclaving inrucion). Conac Marix Technical Service Deparmen a:

18 Troublehooing The moor ound rough and apiraion i very low. Probable Caue Recommended Soluion The pipeor ha been e o he lowe peed Moor ha no been ued for an exended period. Verify peed eing, he lowe peed eing normally caue he moor o run lowly and louder han uual. Cycle he uni (fill and dipene) five o even ime o re-lube he pion. Afer reaembly, he pipeor leak or doe no fill liquid. Probable Caue Recommended Soluion Diaemble he pipeor and reea he O-ring wa no properly eaed. O-ring. (See Secion F.1 and/or F.2 for pipeor diaembly/reaembly). O-ring wa damaged during reaembly. Cylinder wa no crewed ighly ino he pipeor body. (ingle channel only) Replace he O-ring. (See Secion E.2.2, Pipeor Cleaning and Auoclaving (Relubricaion ep) and Secion E.2.3, Pipeor Reaembly). Securely crew he cylinder ino he pipeor body, finger-ighened. Pipeor doe no ejec pipeor ip properly. Probable Caue Recommended Soluion During reaembly of he colle and ejecor leeve, he meal ejecor rigger ab wa no correcly poiioned behind he plaic ab of he ejecor leeve. (Single channel only) Reaemble he colle and ejecor leeve wih he correc orienaion of he ejecor rigger ab o he ejecor leeve ab. (See Secion F.1.3, Pipeor Reaembly). F. MAINTENANCE Mainenance The family of pipeor ha been pecifically deigned for erviceabiliy, enabling he uer o ervice inrumen wih relaive eae. Under cerain circumance where on-ie ervice i no feaible, he inrumen may need o be reurned o Marix for repair (ee Pipeor Service, Secion G.2 and G3). The require very lile mainenance. The exerior of he pipeor can be cleaned periodically wih a of cloh moiened wih mehyl alcohol. Periodic cleaning of he ip fiing and ejecor leeve may be neceary, uing he procedure for mainenance lied below. In cae where pipeor have been expoed o biohazardou maerial, auoclaving inernal componen i required. Marix Technologie provide he following Prevenive Mainenance/Auoclave Ki: Prevenive Mainenance/Auoclave Ki Replacemen cro ube O-ring lubrican Wooden applicaor O-ring Iem # Decripion Iem # Decripion µl - 6, 8, & µl - 6 & 8 channel channel µl - 6, 8, & 12 channel µl - 16 channel µl - 6, 8, & 12 channel µl - 16 channel µl - 12 channel µl - 16 channel CAUTION! DO NOT AUTOCLAVE enire uni or clean he keypad wih bleach oluion or oher olven. Exreme hea can damage he diplay and oher elecronic componen. Cauic cleaning oluion can damage he keypad. Noe: For auoclaving pecific porion of he pipeor refer o he auoclaving ep in Secion F.1.2 for ingle channel pipeor, or ecion F.2.2 for mulichannel pipeor; or call Marix Technical Service Deparmen for inrucion. Noe: Avoid prolonged expoure o ulraviole ligh. The pipeor houing may become dicolored or damaged

19 Mainenance Mainenance F.1 Single Channel Mainenance F.1.1 Single Channel Diaembly Wih proper ue of he pipeor, he diaembly and cleaning of he pipeor ip i no neceary. However, if fluid i inadverenly drawn ino he ip fiing, hen he lower end of he pipeor hould be diaembled for horough cleaning. To diaemble he lower end of he pipeor: 1. Deach he colle by wiing i couner-clockwie (ee Figure F.1.1a). 2. Remove he colle and ejecor leeve by liding boh piece down over he cylinder (ee Figure F.1.1b). For reaembly laer, noe he poiion of he large pring in he colle. Figure F.1.1a: Deaching he Colle 3. Uncrew cylinder couner-clockwie and remove i from he pipeor body. Noe: Check for he preence of he o-ring on he pion. If i i no on he pion, look inide he cylinder and remove he o-ring. 4. Remove O-ring, O-ring uppor, and 1/2 diameer pring for cleaning by liding hem off of he he pion (refer o Figure F.1.1c). IMPORTANT! Leave he pion on he moor crew, DO NOT ry o diaemble! Figure F.1.1b: Pipeor Tip Diaembly Noe: Check for he preence of he O-ring on he pion. If i i no on he pion, look inide he cylinder and remove he O-ring. Figure F.1.1c: Pipeor Tip Aembly 32 33

20 Mainenance Mainenance F.1.2 Single Channel Cleaning and Auoclaving General Cleaning: Carefully wipe down all diaembled plaic and meal par wih mehyl alcohol or 70% iopropyl alcohol. Deconaminaion/Auoclaving: Deconaminae all diaembled par by wiping hem wih a bleach oluion (5% odium hypochlorie) or by auoclaving he par a 121 C for 20 minue. Re-lubricaion: Lighly coa pion wih O-ring lubricaan and replace or re-inall O-ring on he pion, a decribed in reaembly procedure. Noe: Do no clean he O-ring. Replace he O-ring afer deconaminaion or auoclaving (O-ring and O-ring lubricaion i available from Marix Technical Service). F.1.3 Pipeor Reaembly Afer cleaning or deconaminaing he diaembled par, he pipeor can be reaembled (refer o Figure F.1.1c for par idenificaion and reaembly order). 1. Slide he 1/2 diameer pring, O-ring uppor, and O-ring over he pion and moor crew. (ee Figure F.1.3a). 2. Slide he cylinder over he O-ring and pion aembly and crew ino he pipeor body o ha i i finger igh. Do no over ighen. 3. Aemble colle, 1.0 diameer ejecor pring, and ejecor leeve. Noe: Enure he ab on he ejecor leeve i aligned wih he noch in he colle. 4. Slide he colle, wih pring and ejecor leeve, over he cylinder. Poiion he meal ab of he ejecor rigger behind he ab on he ejecor leeve (ee Figure F.1.3b). 5. Aach he colle ono he pipeor body by wiing lef lighly, hen righ, o iner he ab of he pipeor body ino he lock on he colle (ee Figure F.1.3b). 6. Te he ejecor funcion o enure ha he ejecor leeve move down when he ejecor rigger i preed. F.2 Mulichannel Mainenance F.2.1 Mulichannel Diaembly 1. Remove he color ring by genly prying looe one of he edge near he handle rigger. 2. Ue a Phillip crewdriver o remove all hree houing crew. 3. Carefully remove he plaic cover houing and oberve he layou of he inernal par. Figure F.1.3b: Deaching he Colle Figure F.1.3a: O-ring and pion aembly Figure F.2.1a: Removing Cover Houing 34 35

21 Mainenance Mainenance 4. Remove plaic ejecor rigger wih pring and lide he aluminum ejecor comb off of he ip fiing (ee Figure F.2.1b). On 16 channel model, revere hi proce and remove comb fir, hen rigger. Figure F.2.1b: Removing Ejecor Trigger and Ejecor Comb 5. Remove he pion/ cylinder aembly by pulling he moor crew down from he moor haf coupling and ou of he main houing. Remove he manifold aembly by firmly lifing i ou of he main houing uppor rib (ee Figure F.2.1c). Figure F.2.1c: Removing Pion/Cylinder and Manifold Aemblie Iem Decripion Iem Decripion 1. Keypad 15. Tip fiing 2. Main board 16. Conole cover 3. Flex circui 17. Conole diplay 4. Baery box 18. Baerie 5. Driver board 19. Main houing 6. Moor 20. Trigger hinge pin 7. Color ring 21. Handle rigger 8. Moor coupling 22. Handle rigger pring 9. Moor crew 23. Ejecor rigger 10. Pion backplae 24. Pion 11. Phooenor 25. O-ring 12. Cylinder 26. Cro ube 13. Cover houing 27. Manifold plae 14. Houing crew Figure F.2.1d: Pipeor Componen 36 37

22 Mainenance Mainenance F.2.2 Mulichannel Cleaning and Auoclaving 1. Remove he pion aembly from he cylinder. 2. Remove and dicard he cro ube ino a biohazardou conainer. 3. Remove he O-ring (only if neceary, ee ecion F.1 and/or F.2 for O-ring Replacemen) and dicard hem ino a biohazardou conainer. 4. Place all componen (ee Figure F.2.2) ino he auoclave and auoclave a 121 C for 20 minue. 2. Inall new O-ring. Afer he old O-ring have been removed and all componen have been auoclaved, he new O-ring may be inalled. Before inalling he O-ring, apply a hin coa of lubrican o each O-ring, uing he O-ring lubrican upplied in he Prevenive Mainenance/ Auoclave Ki. Figure F.2.3a: Removing O-ring F.2.3 Mulichannel Mainenance and Reaembly Once componen are auoclaved and compleely dry, ue he following procedure o relubricae and reaemble he uni: O-ring Replacemen Figure F.2.2: Componen o be Auoclaved The O-ring may require replacemen if liquid have been over apiraed. In hi cae, he O-ring hould be removed prior o auoclaving. Spare O-ring pack are available hrough Marix for all pipeor volume. Re-lubricaion 1250µl, 850µl, 250µl Pipeor: Ue he wooden applicaor and O-ring lubrican upplied in he Prevenive Mainenance/Auoclave Ki o apply a hin layer of lubricaion approximaely 0.5 inche (approx. 1.3 cm) down ino each pion hole ha require i. Spread he lubrican evenly along he inner wall of each cylinder by uing a circular moion wih he wooden applicaor (ee Figure F.2.3b). 1. Remove old O-ring. 1250µl, 850µl, 250µl Pipeor: O-ring can be removed from he pion by queezing one ide of he O-ring and lifing i ou of he O-ring groove on he pion. Once removed, he O-ring hould be dicarded a biohazardou wae. 125µl, 30µl, 12.5µl Pipeor: O-ring from hee pipeor are locaed in he cylinder. To remove he O-ring, uncrew he four crew ha hold he cylinder cap o he cylinder (ee Figure F.2.3a). Once removed, he O-ring hould be dicarded a biohazardou wae. Figure F.2.3b: Re-lubricaing µl Pipeor 125µl, 30µl, 12.5µl Pipeor: Spread he lubrican direcly ono he pion, approximaely 0.5 inche (approx. 1.3 cm) from he boom of each pion (ee Figure F.2.3c) Figure F.2.3c: Re-lubricaing µl Pipeor 38 39

23 Mainenance Reaembly 1. Reiner he pion aembly ino he lubricaed cylinder. Enure ha he moor crew engage ino he hole in he cener of he cylinder (ee Figure F.2.3d). NOTE: To correcly orien he pion and cylinder aembly, enure ha he lo number of he cylinder i facing up and he pained ecion of he pion backplae i facing down. Alo, care hould be aken o keep he O-ring from being pinched when inering he pion ino he cylinder. 8 Ch Cylinder 12 Ch Cylinder 16 Ch Mainenance Tip Fiing Poiion Cylinder Poiion 16 Channel Channel Channel Channel Channel Channel Channel Channel Table F.2.3b: Cylinder/Cro Tube Poiion Figure F.2.3d: Reaemble Pion/Cylinder Aembly 2. Cro Tube Replacemen Refer o he following figure and able for proper connecion of cro ube from he ip fiing o he cylinder poiion. Noe: If he Cro Tube orienaion beween he cylinder and Tip Fiing i incorrec, reaembly of he pipeor will be impaired. 3. Carefully iner he fla end of he moor crew ino he hole of he moor coupling. Thi can be accomplihed by holding he pion/cylinder aembly a a ligh angle while inering he moor crew ino he hole of he moor coupling. Slowly pin he moor crew unil he fla end of he moor crew i fully engaged ino he moor coupling hole (ee Figure F.2.3e). The cylinder can now be eaed beween he plaic rib in he main houing. NOTE: The lo number on he face of he cylinder hould be facing oward you and he pained ecion of he pion backplae hould be facing he phooenor. The pained ecion i ued by he phooenor o rack he locaion of he pion. Table F.2.3a: Cylinder 40 41

24 Mainenance Mainenance 6. Hold he ejecor comb in place while inering he ejecor rigger (ee Figure F.2.3h). Place he end of he ejecor pring again he plaic op and compre he pring while poiioning he ejecor rigger over he aluminum ejecor comb (he rib on he ejecor rigger hould mae wih he lo on he aluminum ejecor comb). For 16 channel model, inall he rigger prior o connecing he ejecor comb. Figure F.2.3e: Inalling Pion/Cylinder Aembly 4. Afer inalling he pion/cylinder aembly, inall he manifold aembly ino he main houing. To keep he ubing free from ejecor conac, roae he manifold plae and ubing one complee urn counerclockwie (ee Figure F.2.3f). Iner he manifold aembly ino he main houing uppor rib. Figure F.2.3h: Inering Ejecor Trigger NOTE: Be careful no o pinch he cro-ube when inering he ejecor rigger. Figure F.2.3f: Roaing Manifold Aembly 5. Slide he ejecor comb over he ip fiing wih he lo on he back of he comb expoed (ee Figure F.2.3g). Te he ejecor rigger o enure ha he ejecor comb move down when he rigger i preed (hold he ejecor comb firmly in place while eing he rigger). 7. Place he cover houing ono he main houing and faen he hree crew. 8. Re-aach he color ring. 9. Te pipeor for mooh operaion. (Reaemble and repea ep 3-7 if necceary.) The pipeor i now ready for ue. 42 Figure F.2.3g: Aaching Ejecor Comb 43

25 Mainenance Mainenance F.3 Inalling/Replacing Baerie The come wih rechargeable Nickel Meal Hydride (NiMH) baerie or wih a Lihium Ion baery for 16 channel model. Under normal ue, he baerie can be recharged approximaely ime. When hey can no longer hold heir charge, he baerie mu be replaced. Replacemen baerie can be purchaed hrough he Marix Technical Service Dep. The pipeor may alo be reurned o Marix for baery replacemen. Replacemen baerie: 3. Diconnec wire connecor. Pull apar he wo wire connecor over he baery pack (ee Figure E.3.1Ab). 4. Remove baery pack. Pull genly on he baery wire connecor (wihou he pin) o dilodge he baery pack, hen lif he baery pack ou of he main houing (ee Figure F.3.1Ac). 6, 8, 12 Mulichannel 2 NiMH Iem No.: 6092 Single Channel 1 NiMH Pack Iem No.: Channel only 1 Lihium-Ion Baery Iem No.: 2094 Figure F.3.1Ab: Diconnec Wire Noe: When replacing baerie, enure he pipeor i urned off before removing he baery cae. F.3.1 Inalling/Replacing Nickel Meal Hydride Baerie F.3.1A Inalling/Replacing Single Channel Baerie 1. Remove crew. Ue a mall, flahead crewdriver o remove he wo crew holding he op of he pipeor (conaining he diplay and keypad) o he pipeor houing. The crew are locaed ju above he pipeing rigger (ee Figure F.3.1Aa). 2. Lif pipeor op. Carefully lif he op of he pipeor o expoe he baery pack. Figure F.3.1Aa: Removing Pipeor Cover 5. Iner new baery pack. Iner he new baery pack ino he caviy of he main houing. Enure ha he wire i looped under he baery pack and he connecor i above i (refer o Figure F.3.1Ac). NOTE: The baery pack fi very nugly in he main houing; herefore, enure ha he pack i oriened a hown in Figure F.3.1Ac. The connecor for he baery pack and he pipeor hould be on oppoie ide. 6. Reconnec wire connecor. Reconnec he wire connecor above he baery pack. Genly puh he wire of he baery pack down unil he wire connecor lay fla over he op of he baery pack. Figure F.3.1Ac: Remove Baery Figure F.3.1Ad: Reconnec Wire 44 45

26 Mainenance Mainenance 7. Reaemble pipeor op. Aach pipeor op o he main houing. Enure flex cable i behind he op of he pipeor rigger and he rigger conac he limi wich properly (ee Figure F.3.1Ae). Fi he edge of he pipeor op nugly wihin he edge along he op of he pipeor houing. Enure ha he cable o he power jack are inide he main houing well below he edge o avoid pinching he cable. Hold aembly ogeher firmly while reecuring he wo crew. 8. Recharge baerie. Recharge he new baerie before uing he pipeor (ee Secion F.4, Recharging Baerie). Figure F.3.1Ae: Aembling Pipeor Top 1. Remove baery cae. Poiion your humb over he humb grip (ee Figure F.3.1Ba) and brace your finger again he fron of he keypad. Apply firm preure o he panel wih he humb grip o releae he lach. 2. Remove baerie. Pre a pipe ip again eiher baery hrough he lo in he boom of he baery cae o remove he baerie (ee Figure F.3.1Bb). 3. Inall new baerie. Iner he pacer ino he boom of he baery box (a hown in Figure F.3.1Bc). Iner Figure F.3.1Bb: Opening he Cae new baerie by placing he poiive end ino he baery cae fir, hen he negaive end in place over he pacer (an exploded view of he aembly i hown in Figure F.3.1Bc, or he diagram in he baery cae). Enure ha he baerie are correcly oriened. F.3.1B Inalling/Replacing Mulichannel Baerie The pipeor i hipped wih Nickel Meal Hydride baerie packaged eparaely. Inall he baerie and recharge hem for hour. Ue he following procedure o inall he baerie, hen coninue o Secion F.4, Recharging Baerie. Figure F.3.1Ba: Removing Baery Cae 46 Figure F.3.1Bc: Inalling NiMH Baerie 47

27 Mainenance Mainenance F.3.2 Inalling/Replacing Lihium-Ion Baery (16 Channel ) Your pipeor i hipped wih a Lihium-Ion Baery. Before uing he pipeor, charge i for 2 hour. Ue he following procedure when inalling new baerie. Oherwie, coninue o Secion F.4, Recharging Baerie. 1. Remove baery cae. Poiion your humb over he humb grip (ee Figure F.3.2a,b) and brace your finger again he fron of he keypad. Apply preure o he panel wih he humb grip o releae lach. Carefully lide baery cae ou of he pipeor, allowing ufficien lack for aached wire of he baery. 3. Carefully Remove Conole Cover. Pre and lif off conole cover aembly (ee Figure F.3.2e). BE CAREFUL no o ear he aached flex cable! (Refer o Figure F.3.2f). 4. Unclap Baery Connecor. Lif one ide Figure F.3.2e: Remove Conole Cover of baery connecor wih a mall flahead crewdriver. Grap all hree wire and pull ou connecor (ee Figure F.3.2f,g). Figure F.3.2a: Baery Cae (Cloed) Figure F.3.2b: Baery Cae (Open) 2. Remove Conole Cover. Unclip he wo nap on conole cover aembly, hen unhook he end of conole cover aembly (ee Figure F.3.2c,d). Figure F.3.2f: Lif Baery Connecor CAUTION: Be careful no o ear he Flex Cable Figure F.3.2c: Unclip Snap Figure F.3.2d: Unhook Cover Figure F.3.2g: Unclap Baery Connecor 48 49

28 Mainenance Mainenance 5. Connec New Baery. Aach he wire of he new baery in place of he old baery. 6. Reaemble Conole Cover. Aach he pipeor op o he main houing. Enure ha he flex cable i behind he op of he pipeor rigger and he rigger conac he limi wich properly (refer o Figure F.3.2a hru F.3.2g a needed). Fi he edge of he pipeor op nugly wihin he edge along he op of he pipeor houing. Enure he cable o he power jack are inide he main houing, well below he edge, o avoid pinching he cable. F.4 Charging/Recharging Baerie F.4.1 Charging/Recharging Nickel Meal Hydride (NiMH) Baerie The ue wo Nickel Meal Hydride (NiMH) rechargeable baerie, locaed in a baery cae under he keypad. The baerie are charged in he pipeor by uing he power ranformer included in he package, wih he excepion of ingle channel pipeor which do no have hi abiliy. You can operae he pipeor while i i recharging; or you can operae i olely wih he power ranformer (wihou baerie). The baerie can be recharged approximaely ime. To exend baery life, he pipeor will urn off auomaically if i i no ued wihin en minue. + Noe: Baery charge i low when he baery ymbol appear on he diplay. The pipeor will coninue o funcion, bu hould be recharged a he earlie convenien ime. 1. Aach ranformer. Aach he cable end of he ranformer ino he pipeor. The plug recepacle i above he color ring. Plug he ranformer ino he elecrical oule. 2. Recharge baerie. Recharge baerie for hour o enure a full charge. Afer he baerie have been recharged, unplug he pipeor from he ranformer. Noe: Baerie hold heir maximum charge if hey are fully dicharged before recharging again. However, overnigh recharging i accepable if he pipeor i in normal ue during he day. CAUTION! Avoid exceive charging of he baerie when he pipeor i no in ue. Exceive charge o he baerie will horen he baery life and may damage he baerie. F.4.2 Charging/Recharging Lihium-Ion Baery (16 Channel ) The ue a Lihium-Ion rechargeable baery, locaed in a baery cae under he keypad. The baery i charged while i i in he pipeor by uing he power ranformer included in he package. The pipeor can be operaed while i i recharging; or i can be operaed olely wih he power ranformer (wih no baery). The baery can be recharged approximaely ime. To exend baery life, he pipeor will urn off auomaically if i i no ued wihin en minue. + Noe: Baery charge i low when he baery ymbol appear on he diplay. The pipeor will coninue o funcion, bu hould be recharged a he earlie convenien ime. Rapid Charge - When he low baery indicaor appear (on he pipeor LCD diplay creen) he baery hould be charged for 1.5 o 2 hour o achieve a full charge. Refer o he following able for parial charge ime: Amoun of Charge Time 80% 1.25 hour 95% 1.5 hour 100% 2 hour max The baery pack for he 16 Channel feaure an indicaor ligh o indicae he ae of charge of he baery while plugged ino he power upply. Wih he uni plugged in, he indicaor operae a follow: Green - Indicae ha he power upply i conneced. Orange - Indicae charging (charge curren ino he baery). Flahing Orange o Green - When he baery i fully drained he indicaor will remain a olid orange for a while a he baery require conan charge curren

29 Mainenance A he uni coninue o charge, charge curren i upplied in pule. Thi i indicaed by he pulaion of he indicaor. A he baery near full charge he pulaion will low down becoming nearly a conan green. When he baery i fully charged he indicaor will be almo a conan green, flahing only abou once every 3-5 econd o enure he baery ay opped off wih full charge. The baery i now in a fully charged ae. Noe: The ypical life expecancy of he baery i approximaely full recharge. To prolong hi and aain he maximum uage from he baery i i recommended o recharge he baery whenever i i no being ued inead of waiing unil he Low-Ba ymbol appear. Compleely draining he baery will no horen he life expecancy. However, recharging before fully dicharged will prolong he baery life. CAUTION! Avoid exceive charging of he baerie when he pipeor i no in ue. Exceive charge o he baerie will horen he baery life and may damage he baerie. CAUTION! The baery pack and power upply for 16 channel i no inerchangeable wih he power upply and baery pack of oher model. The power jack/connecor and baery connecor are differen o preven he maing wih oher model. G. TECHNICAL SERVICE G.1 Inrumen Warrany Thi inrumen i warraned o he original purchaer by Marix Technologie Corporaion o be free of defec in maerial or workmanhip for a period of 12 monh from he dae of purchae, providing ha i ha been operaed according o he inrucion, no abued or miued, ha he erial number ha no been removed, and ha he inrumen ha no been diaembled, oher han for procedure for normal mainenance. No oher warrany i expreed or implied, including he warrany of merchanabiliy. No inrumen hould be reurned wihou a prior Reurn Good Auhorizaion from Marix. Should a uni need o be reurned, he purchaer mu pay inurance and hipping charge. Marix Technologie will aume he co of reurning he repaired uni o he purchaer. Thi produc packaging i capable of wihanding normal hipping hazard. If an inrumen need o be repaired, pleae reurn i o u in i original hipping caron, if poible. Conac Marix Technical Service for recommended equipmen deconaminaion mehod (ee alo ecion G.4), or o obain repair or replacemen wihin he erm of he above warrany a (888) , fax: (603) For Europe and Aia locaion, pleae vii: hp://

30 Technical Service Technical Service G.2 On Sie Service - Calibraion & Mainenance G.3 Facory Service - Calibraion, Mainenance, & Repair A Your Service Exper Pipeor Service On-ie, exper pipeor prevenive mainenance and calibraion ervice. Trued Service Cerified, Marix-rained echnician calibrae & repair mo pipeor brand and model. Cerified Marix-rained echnician calibrae and ervice mo manual and elecronic pipeor brand and model Marix, Biohi, Rainin, Eppendorf, Gilon, Oxford, and more. Marix ha 20-plu year manufacuring & ervicing manual and elecronic pipeor. Broad range of ervice o fi every need: Baic Calibraion, Eenial Prevenive Mainenance, and Repair Service. Baic Calibraion Phyical pipeor inpecion. Sreamlined Proce Leak e. Marix pecially deigned pipeor hipping boxe are convenien and offer high viibiliy. Replacemen of o-ring and eal, a needed. Gravimeric performance validaion uing NIST-raceable balance. Sreamlined documenaion feaure one form wih ervice opion, deconaminaion cerificaion, and hipping deail. Performance validaion of 5 aliquo a wo volume verified again performance pecificaion aed in ISO 8655 andard. Conac your Marix repreenaive or call o have pre-addreed and pre-paid pipeor hipping boxe delivered righ o your door. Calibraion adjumen, a needed. Performance validaion repor. Pipeor Shipping Prevenive Mainenance and Cuom Calibraion i available upon reque. Marix calibraion ervice combine uperior ervice and fa urnaround o ge your pipeor operaing a peak performance wihou delay. Schedule a Clinic Today! Send one or muliple pipeor, uing he packaging and carrier of your choice. If you have 50 or more Pipeor, Call or oday o arrange a clinic. Each peron who bring 20 pipeor o he clinic receive a free gif! Accuracy & Preciion Marix Service Pledge Single Channel pipeor are qualified wih 5 aliquo verified on NIST-raceable gravimeric balance a wo volume. Our echnical exper e he benchmark in pipeor ervice. If a pipeor fail validaion on-ie, we will offer o repair i a our faciliy and, if neceary, deal wih he original manufacurer o enure any major repair are compleed properly. Mulichannel pipeor are qualified wih 5 aliquo verified gravimerically a high and low volume uing one channel. The preciion of he pipeor i meaured by a pecrophoomic aborbance verificaion. Thi added e will allow you o ee he performance of each channel of your muli channel pipeor a i dipene he lowe e volume acro a microplae. No hale or preure i Marix ervice. Marix and he Marix logo are regiered rademark of Marix Technologie Corporaion. All rademark and produc name are he inellecual/indurial propery of he repecive owner. 54 The accuracy and preciion of each pipeor will be verified in a emperaure and humidiy conrolled environmen o mee he performance pecificaion aed in he ISO 8655 andard. Addiionally, if you require a received performance daa or ISO complian eing proocol, Marix will gladly provide cuom calibraion ervice upon reque. 22 Friar Drive, Hudon, NH Impac2 Impac2 55

31 Technical Service Technical Service G.4 Recommended Equipmen Deconaminaion Mehod 56 The following able li he proper deconaminaion procedure for reurning equipmen o Marix for ervice. Produc/Componen Hea a 65 C/ 10 Hr. 70%/30% IPA/Waer 10% Bleach Ehylene Oxide Auoclave 121 C/ 15 min. Muli Impac/, EXP, EQUALIZER, 16 CHANNEL Pipeor Exerior YES Wipe Wipe YES NO Pion/Cylinder Aembly YES YES NO YES YES Tip Manifold YES YES NO YES YES Baery NO Wipe NO NO NO Power Supply NO Wipe NO NO NO Pipeor Exerior NO Wipe Wipe NO NO Single Ch. Impac/ Pipeor w/baery Removed Ye Wipe Wipe YES YES Lower cylinder YES YES YES YES YES Pion (do no remove) YES YES NO YES NO Power Supply NO Wipe NO NO NO Elecrapee Houing Exerior YES Wipe Wipe YES NO Power Supply NO Wipe NO NO NO Houing Exerior YES Wipe NO YES NO Memowell Power Supply NO Wipe NO NO NO Houing Exerior YES Wipe NO YES YES Manual Pipeor Lower aembly YES Wipe NO YES YES Houing Exerior YES Wipe Wipe YES NO Cellmae II Noecone YES YES YES YES YES Silicone Rubber Holder YES YES Wipe YES YES Filer Replace Replace Replace Replace Replace Baery NO Wipe NO NO NO Power Supply NO Wipe NO NO NO Houing Exerior YES Wipe NO NO NO WARNING: Do no expoe baerie o emperaure above 60 C. G.5 Equipmen Deconaminaion Verificaion Form Serial # RP# PO# Prior o ervice of equipmen ha may be conaminaed wih biohazardou maerial, poenially biohazardou maerial or radioaciviy, he uer mu fir deconaminae i. Thi deconaminaion procedure will include he following a appropriae (pleae check one): Thi equipmen ha no been ued in a laboraory/locaion expoing i o biohazard or radioacive maerial. Thi equipmen wa APPROPRIATELY DECONTAMINATED* FROM BIOHAZARDOUS MATERIALS WITH: *Example: Auoclave, 10% Bleach, Ehylene Oxide. Inrumen mu be deconaminaed boh exernally and inernally. Thi equipmen wa APPROPRIATELY DECONTAMINATED & TESTED FOR RADIOACTIVITY* BY: *Example: wipe e wih reul (3H, 14C,a-emier), Geiger coun, ec. NAME (prin) PHONE # SIGNATURE DATE Federal regulaion require ha all inrumen en for ervice hall be free of radioaciviy and biohazard. The Technical Service group reerve he righ o refue o work on hi equipmen if hee procedure are no performed. Cuomer Reurn Shipping Addre: Reurn fax# FRM029 Rev. D 57

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