Exercise & Cellular Respiration
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1 Exercise & Cellular Respiration Name: Block: Background Information. Cellular respiration (see chemical reaction below) is a chemical reaction that occurs in your cells to create energy; when you are exercising your muscle cells are creating ATP to contract. Cellular respiration requires oxygen (which is breathed in) and creates carbon dioxide (which is breathed out). C 6H 12O 6 + 6O 2 6H 2O + 6CO ATP (energy) This lab will address how exercise (increased muscle activity) affects the rate of cellular respiration. You will measure 3 different indicators of cellular respiration: breathing rate, heart rate, and carbon dioxide production. You will measure these indicators at rest (with no exercise) and after 1 and 2 minutes of exercise. Breathing rate is measured in breaths per minute, heart rate in beats per minute, and carbon dioxide in the time it takes the sodium carbonate solution to change color. Carbon dioxide production can be measured by breathing through a straw into a solution of sodium carbonate combined with phenolphthalein. Phenolphthalein is an acid/base indicator; when it reacts with acid it changes from pink to clear. When carbon dioxide reacts with water, a weak acid (carbonic acid) is formed (see chemical reaction below). The more carbon dioxide you breathe into the solution, the faster it will change color to clear. Materials: - Small beaker - sodium carbonate solution 6CO H 2O 6 HCO H + - straw - stopwatch/timer Pre-Lab Questions Answer the following questions using the information above AND your Chapter 9 Section 9.1 Screencast Notes. 1. Where will you find all the energy in food molecules? How do you access that energy? 2. What is the purpose of Cell Respiration? 3. What is REQUIRED if cell respiration is going to be carried out? 4. Write the chemical reaction for Cell Respiration below. 5. There are 3 main stages in Cell Respiration, but only 2 require oxygen. List each stage and use your screencast notes to describe what is produced during each stage, including the number of ATP s being made. 6. What is the total number of ATP s that can be produced if all 3 stages are used? 7. The processes seen in Cell Respiration can be described as either: a. Aerobic: a process that requires b. Anaerobic: a process that DOES NOT require 8. Two of the 3 processes that can occur in Cell Respiration occur in the and these two processes are: a. b. 9. The 3 rd process,, happens in the.
2 Hypothesis (write a hypothesis about how you think exercise will affect the amount of carbon dioxide you produce) If Then Because PART A: Resting (no exercise) Measuring Carbon Dioxide Production: 1. Add 25 ml of pink Sodium Carbonate Solution to a small flask. Using a straw, exhale into the solution. (CAUTION: Do not inhale the solution!) RECORD the time it takes for the solution to turn clear in your data table. 2. Perform the procedure in step #1, two more times. RECORD your results. 4. Next, calculate the average for each student. 5. Then, calculate the average for the averages calculated in step 4. Measuring Breathing Rate: 1. Count the number of breaths (1 breath = inhale + exhale) you take in 1 minute. RECORD this in your data table 2. Do this again two more times. RECORD your results. 4. Next, calculate the average for all students. Measuring Heart Rate: 1. Take your pulse by counting the number of beats in 30 seconds and multiply that number by 2. RECORD this in your data table. 2. Do this again two more times. RECORD your results. 4. Next, calculate the average for all students. PART B: Increased Muscle (Exercise) 1. Exercise for exactly 1 minute by doing jumping jacks. 2. After 1 minute of exercise, immediately exhale through the straw into the solution. Time how long it takes for the solution to turn clear. Record this in your data table. 3. Quickly calculate your breathing and heart rates as you did before. You only need to do this once. 4. Record this in your data table. Get more of the sodium carbonate solution. 5. Exercise as you did before, but for 5 continuous minutes. 6. Immediately exhale through the straw into the solution. Time how long it takes for the pink solution to turn clear. RECORD the time in your data table. 7. After one student has performed this procedure, collect the date from two other students and RECORD. 8. Calculate the average for 1 minute and 5 continuous minutes of exercise. NOW, Use the information in your data table to create a 3 BAR graphs: carbon dioxide production vs. activity, Breathing Rate vs., and Heart Rate vs.. ONLY GRAPH THE AVERAGES!!! FOR RESTING, 1 MINUTE EXERCISE, AND 5 MINUTES OF CONTINUOUS EXERCISE. Table 1. Carbon Dioxide Production (time to turn Phenolphthalein solution clear) Student 1 Student 2 Student 3
3 Trial 1 EXERCISE 1 minute Table 2. Breathing Rate (Breaths/minute) Student 1 Student 2 Student 3 Trial 1 EXERCISE 1 minute Table 3. Heart Rate (Beats/minute) Trial 1 EXERCISE 1 minute Student 1 Student 2 Student 3
4 Analysis & Conclusions: Answer the questions below using your BACKGROUND information in the lab, your lab data, and Chapter 9 Section 9.1 Screencast Notes.. 1. How did exercise affect the time needed for the solution to change color? Explain why the color change occurred 2. What can you conclude about the effect of exercise on the amount of carbon dioxide that is present in your exhaled breath? Why is this so? 3. What can you conclude about the effect of exercise on breathing rate? Why is this so?
5 4. What can you conclude about the effect of exercise on heart rate? Why is this so? 5. What do your muscles need during exercise? How does this material get to your muscles? 6. Discuss whether your hypothesis was supported or not supported. Use NUMERICAL data in your discussion and use all elements of collected data (Phenolphthalein indicator, breathing rate, and heart rate). 7. What do you think is going on in your muscles as the intensity of the activity is increased?
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