SQK-NSK007-Nanopore Sequencing KiW
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1 SQK-NSK007-Nanopore Sequencing KiW
2 Revision Date 03/2014 SAFETY DATA SHEET LAMBDA DNA According to Regulation (EU) No 453/2010 SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING 1.1. Product identifier Product name LAMBDA DNA Container size 88 µl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Laboratory Reagent. For research purposes only Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA UK T: +44 (0) F: +44 (0) W: E: support@nanoporetech.com 1.4. Emergency telephone number +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: HAZARDS IDENTIFICATION 2.1. Classification of the substance or mixture Classification (EC 1272/2008) Classification (1999/45/EEC) Physical and Chemical Hazards Not classified. Human health Environment Not classified. Not classified. Not classified. The Full Text for all R-Phrases and Hazard Statements are Displayed in Section 16. Human health Warning - this preparation contains a substance not yet tested completely. Environment The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment Label elements Label In Accordance With (EC) No. 1272/2008 No pictogram required. Precautionary Statements 2.3. Other hazards P280 Wear protective clothing, gloves, eye and face protection. This product does not contain any PBT or vpvb substances. SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTS 3.2. Mixtures 1 / 7
3 LAMBDA DNA Composition Comments No classified ingredients, or those having occupational exposure limits, present above the levels of disclosure. SECTION 4: FIRST AID MEASURES 4.1. Description of first aid measures Warning - this preparation contains a substance not yet tested completely. Get medical advice/attention if you feel unwell. Inhalation Due to the small packaging the risk of inhalation is minimal. Move into fresh air and keep at rest. Get medical attention if any discomfort continues. Ingestion Get medical attention if any discomfort continues. Skin contact Remove contaminated clothing. Wash skin thoroughly with soap and water for several minutes. Get medical attention promptly if symptoms occur after washing. Eye contact Make sure to remove any contact lenses from the eyes before rinsing. Promptly wash eyes with plenty of water while lifting the eye lids. Continue to rinse for at least 15 minutes. Get medical attention if any discomfort continues Most important symptoms and effects, both acute and delayed Inhalation No specific symptoms noted. Ingestion Not known. May cause discomfort if swallowed. Skin contact No specific symptoms noted. Eye contact Spray in the eyes: May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed The severity of the symptoms described will vary dependant of the concentration and the length of exposure. Warning - this preparation contains a substance not yet tested completely. SECTION 5: FIREFIGHTING MEASURES 5.1. Extinguishing media Extinguishing media This product is not flammable. Use fire-extinguishing media appropriate for surrounding materials. Unsuitable extinguishing media Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Hazardous combustion products Fire creates: Carbon monoxide (CO). Carbon dioxide (CO2). Hydrocarbons. Unusual Fire & Explosion Hazards No unusual fire or explosion hazards noted. Specific hazards Due to the small packaging, the risk of inhaling gases formed in a fire is minimal Advice for firefighters Special Fire Fighting Procedures Warning - this preparation contains a substance not yet tested completely. Avoid contact with skin and eyes. Protective equipment for fire-fighters Self contained breathing apparatus and full protective clothing must be worn in case of fire. SECTION 6: ACCIDENTAL RELEASE MEASURES 6.1. Personal precautions, protective equipment and emergency procedures Wear protective clothing as described in Section 8 of this safety data sheet Environmental precautions Avoid discharge to the aquatic environment. 2 / 7
4 6.3. Methods and material for containment and cleaning up LAMBDA DNA In case of spills, beware of slippery floors and surfaces. Wear necessary protective equipment. Absorb spillage with non-combustible, absorbent material. Transfer to a container for disposal. For waste disposal, see section Reference to other sections Wear protective clothing as described in Section 8 of this safety data sheet. See section 11 for more detailed information on health effects and symptoms. For waste disposal, see section 13. SECTION 7: HANDLING AND STORAGE 7.1. Precautions for safe handling Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Do not handle broken packages without protective equipment. Avoid contact with skin and eyes. Do not eat, drink or smoke when using the product. Wash hands thoroughly after handling Conditions for safe storage, including any incompatibilities Protect from freezing and direct sunlight. Store at temperatures not exceeding 4 C Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION 8.1. Control parameters Ingredient Comments No exposure limits noted for ingredient(s) Exposure controls Process conditions Provide eyewash station. Engineering measures Provide adequate ventilation. Respiratory equipment No specific recommendations. Hand protection Protective gloves must be used if there is a risk of direct contact or splash. The most suitable glove must be chosen in consultation with the gloves supplier, who can inform about the breakthrough time of the glove material. Eye protection Wear approved safety goggles. Other Protection Wear suitable protective clothing as protection against splashing or contamination. Hygiene measures Wash hands after handling. When using do not eat, drink or smoke. Wash contaminated clothing before reuse. Environmental Exposure Controls Avoid discharge to the aquatic environment. SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES 9.1. Information on basic physical and chemical properties Appearance Colour Odour Solubility Initial boiling point and boiling range ( C) Melting point ( C) Relative density Not determined. Vapour density (air=1) Not determined. Liquid Colourless. No characteristic odour. Soluble in water mm Hg ~0 760 mm Hg 3 / 7
5 Vapour pressure Not determined. Evaporation rate Not determined. ph-value, Conc. Solution Not determined. Viscosity Not determined. Decomposition temperature ( C) Not determined. Odour Threshold, Lower Not determined. Odour Threshold, Upper Not determined. Flash point ( C) Not determined. Auto Ignition Temperature ( C) Not determined. Flammability Limit - Lower(%) Not determined. Flammability Limit - Upper(%) Not determined. Partition Coefficient (N-Octanol/Water) Not determined. Explosive properties Not considered to be explosive. Oxidising properties Does not meet the criteria for oxidising. LAMBDA DNA 9.2. Other information Not known. SECTION 10: STABILITY AND REACTIVITY Reactivity There are no known reactivity hazards associated with this product Chemical stability Stable under normal temperature conditions and recommended use. Stable under the prescribed storage conditions Possibility of hazardous reactions Not known. Hazardous Polymerisation Will not polymerise Conditions to avoid Avoid heat. Protect from freezing and direct sunlight Incompatible materials Materials To Avoid No incompatible groups noted Hazardous decomposition products Not known. SECTION 11: TOXICOLOGICAL INFORMATION Information on toxicological effects Toxicological information Warning - this preparation contains a substance not yet tested completely. 4 / 7
6 LAMBDA DNA Acute toxicity: Acute Toxicity (Oral LD50) No information available. Acute Toxicity (Dermal LD50) No information available. Acute Toxicity (Inhalation LC50) No information available. Skin Corrosion/Irritation: Serious eye damage/irritation: Respiratory or skin sensitisation: Respiratory sensitisation No information available. Skin sensitisation No information available. Germ cell mutagenicity: Genotoxicity - In Vitro No information available. Carcinogenicity: Carcinogenicity No information available. Reproductive Toxicity: Reproductive Toxicity - Fertility No information available. Reproductive Toxicity - Development No information available. Specific target organ toxicity - single exposure: STOT - Single exposure No information available. Specific target organ toxicity - repeated exposure: STOT - Repeated exposure No information available. Aspiration hazard: Not anticipated to present an aspiration hazard based on chemical structure. SECTION 12: ECOLOGICAL INFORMATION Ecotoxicity The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment Toxicity 5 / 7
7 Acute Fish Toxicity Not considered toxic to fish. LAMBDA DNA Persistence and degradability Degradability There are no data on the degradability of this product Bioaccumulative potential Bioaccumulative potential No data available on bioaccumulation. Partition coefficient Not determined Mobility in soil Mobility: The product is soluble in water Results of PBT and vpvb assessment This product does not contain any PBT or vpvb substances Other adverse effects None known. SECTION 13: DISPOSAL CONSIDERATIONS Waste treatment methods When handling waste, consideration should be made to the safety precautions applying to handling of the product. Residues and empty containers should be taken care of as hazardous waste according to local and national provisions. SECTION 14: TRANSPORT INFORMATION General The product is not covered by international regulation on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) Transport Labels No transport warning sign required Packing group Environmental hazards Environmentally Hazardous Substance/Marine Pollutant No Special precautions for user Transport in bulk according to Annex II of MARPOL73/78 and the IBC Code SECTION 15: REGULATORY INFORMATION 6 / 7
8 LAMBDA DNA Safety, health and environmental regulations/legislation specific for the substance or mixture Guidance Notes Workplace Exposure Limits EH40. EU Legislation Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006 with amendments Chemical Safety Assessment No chemical safety assessment has been carried out. SECTION 16: OTHER INFORMATION Only trained personnel should use this material. Revision Comments This is first issue. Revision Date 03/2014 Risk Phrases In Full NC Not classified. 7 / 7
9 Revision date: 12/08/2015 SAFETY DATA SHEET Elution Buffer According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product name Container size Elution Buffer 200μl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Laboratory reagent. No specific uses advised against are identified Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA, UK T: +44 (0) F: +44 (0) E: support@nanoporetech.com 1.4. Emergency telephone number Emergency telephone +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification Physical hazards Not Classified Health hazards Environmental hazards Not Classified Not Classified 2.2. Label elements Hazard statements Precautionary statements Important Notes. NC Not Classified P280 Wear protective clothing, gloves, eye and face protection. This product is considered to be a small package and is labelled according to the relevant provisions of the legislation Other hazards This product does not contain any substances classified as PBT or vpvb. SECTION 3: Composition/information on ingredients 3.2. Mixtures 1/8
10 Revision date: 12/08/2015 Elution Buffer Composition comments No classified ingredients, or those having occupational exposure limits, present above the levels of disclosure.,this product contains a substance that has not yet been fully tested and may have unforeseen effects. SECTION 4: First aid measures 4.1. Description of first aid measures Inhalation Ingestion Skin contact Eye contact Protection of first aiders Not considered to be a significant hazard due to the small quantities used. Get medical attention if any discomfort continues. Show this Safety Data Sheet to the medical personnel. Move affected person to fresh air and keep warm and at rest in a position comfortable for breathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Rinse mouth thoroughly with water. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Give a few small glasses of water or milk to drink. Stop if the affected person feels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction of medical personnel. Remove affected person from source of contamination. Rinse immediately with plenty of water. Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wide apart. Continue to rinse for at least 10 minutes. First aid personnel should wear appropriate protective equipment during any rescue Most important symptoms and effects, both acute and delayed Inhalation Ingestion Skin contact Eye contact See Section 11 for additional information on health hazards. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Not considered to be a significant hazard due to the small quantities used. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed Notes for the doctor Specific treatments Treat symptomatically. No special treatment required. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire-extinguishing media suitable for the surrounding fire. Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Specific hazards Hazardous combustion products Containers can burst violently or explode when heated, due to excessive pressure build-up. Not considered to be a significant hazard due to the small quantities used. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours Advice for firefighters 2/8
11 Revision date: 12/08/2015 Elution Buffer Protective actions during firefighting Special protective equipment for firefighters Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat with water spray and remove them from the fire area if it can be done without risk. Cool containers exposed to flames with water until well after the fire is out. If a leak or spill has not ignited, use water spray to disperse vapours and protect men stopping the leak. Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protective clothing. Firefighter's clothing conforming to European standard EN469 (including helmets, protective boots and gloves) will provide a basic level of protection for chemical incidents. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keep unnecessary and unprotected personnel away from the spillage. Wear protective clothing as described in Section 8 of this safety data sheet. Follow precautions for safe handling described in this safety data sheet. Wash thoroughly after dealing with a spillage Environmental precautions Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities if environmental pollution occurs (sewers, waterways, soil or air) Methods and material for containment and cleaning up Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycle products wherever possible. If the product is soluble in water, dilute the spillage with water and mop it up. Flush contaminated area with plenty of water. Wash thoroughly after dealing with a spillage. Dispose of waste to licensed waste disposal site in accordance with the requirements of the local Waste Disposal Authority Reference to other sections Reference to other sections For personal protection, see Section 8. SECTION 7: Handling and storage 7.1. Precautions for safe handling Usage precautions Advice on general occupational hygiene Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs. Handle all packages and containers carefully to minimise spills. Keep container tightly sealed when not in use. Avoid the formation of mists. Wash promptly if skin becomes contaminated. Take off contaminated clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Wash at the end of each work shift and before eating, smoking and using the toilet. Change work clothing daily before leaving workplace Conditions for safe storage, including any incompatibilities Storage precautions Storage class Store in tightly-closed, original container in a dry, cool and well-ventilated place. Store at temperatures not exceeding 4 C. Unspecified storage Specific end use(s) Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: Exposure Controls/personal protection 8.1. Control parameters Ingredient comments No exposure limits known for ingredient(s). 3/8
12 Revision date: 12/08/2015 Elution Buffer 8.2. Exposure controls Protective equipment Appropriate engineering controls Eye/face protection Hand protection Other skin and body protection Hygiene measures Respiratory protection Environmental exposure controls Provide adequate ventilation. Good general ventilation should be adequate to control worker exposure to airborne contaminants. Eyewear complying with an approved standard should be worn if a risk assessment indicates eye contact is possible. Personal protective equipment for eye and face protection should comply with European Standard EN166. The following protection should be worn: Chemical splash goggles. Chemical-resistant, impervious gloves complying with an approved standard should be worn if a risk assessment indicates skin contact is possible. Considering the data specified by the glove manufacturer, check during use that the gloves are retaining their protective properties and change them as soon as any deterioration is detected. Appropriate footwear and additional protective clothing complying with an approved standard should be worn if a risk assessment indicates skin contamination is possible. Provide eyewash station and safety shower. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reuse. Clean equipment and the work area every day. Good personal hygiene procedures should be implemented. Wash at the end of each work shift and before eating, smoking and using the toilet. When using do not eat, drink or smoke. Respiratory protection complying with an approved standard should be worn if a risk assessment indicates inhalation of contaminants is possible. Provide adequate ventilation. Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn. Not regarded as dangerous for the environment. SECTION 9: Physical and Chemical Properties 9.1. Information on basic physical and chemical properties Appearance Colour Odour Odour threshold ph Melting point Initial boiling point and range Flash point Evaporation rate Upper/lower flammability or explosive limits Vapour pressure Vapour density Liquid. Colourless. Odourless. ~0 760 mm Hg ~ mm Hg 4/8
13 Revision date: 12/08/2015 Elution Buffer Relative density Solubility(ies) Partition coefficient Auto-ignition temperature Decomposition Temperature Viscosity Explosive properties Oxidising properties Soluble in water. Not considered to be explosive. Does not meet the criteria for classification as oxidising Other information Other information No information required. SECTION 10: Stability and reactivity Reactivity Reactivity There are no known reactivity hazards associated with this product Chemical stability Stability Stable at normal ambient temperatures and when used as recommended. Stable under the prescribed storage conditions Possibility of hazardous reactions Possibility of hazardous reactions No potentially hazardous reactions known Conditions to avoid Conditions to avoid Avoid excessive heat for prolonged periods of time Incompatible materials Materials to avoid Strong oxidising agents. Strong reducing agents Hazardous decomposition products Hazardous decomposition products Does not decompose when used and stored as recommended. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours. SECTION 11: Toxicological information Information on toxicological effects Toxicological effects Acute toxicity - oral Notes (oral LD₅₀) Acute toxicity - dermal Notes (dermal LD₅₀) Acute toxicity - inhalation Notes (inhalation LC₅₀) Skin corrosion/irritation Animal data Not regarded as a health hazard under current legislation. Serious eye damage/irritation 5/8
14 Revision date: 12/08/2015 Elution Buffer Serious eye damage/irritation Respiratory sensitisation Respiratory sensitisation Skin sensitisation Skin sensitisation Germ cell mutagenicity Genotoxicity - in vitro Carcinogenicity Carcinogenicity IARC carcinogenicity Reproductive toxicity Reproductive toxicity - fertility Reproductive toxicity - development None of the ingredients are listed or exempt. Specific target organ toxicity - single exposure STOT - single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure STOT - repeated exposure Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard Aspiration hazard Inhalation Ingestion Skin contact Eye contact Route of entry Target organs No specific health hazards known. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation. Ingestion Inhalation Skin and/or eye contact No specific target organs known. SECTION 12: Ecological Information Ecotoxicity Not considered to be a significant hazard due to the small quantities used. Not regarded as dangerous for the environment. However, large or frequent spills may have hazardous effects on the environment Toxicity Toxicity Persistence and degradability Persistence and degradability The degradability of the product is not known Bioaccumulative potential 6/8
15 Revision date: 12/08/2015 Elution Buffer Bioaccumulative potential Partition coefficient No data available on bioaccumulation Mobility in soil Mobility The product is water-soluble and may spread in water systems Results of PBT and vpvb assessment Results of PBT and vpvb assessment This product does not contain any substances classified as PBT or vpvb Other adverse effects Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Disposal methods The generation of waste should be minimised or avoided wherever possible. Reuse or recycle products wherever possible. This material and its container must be disposed of in a safe way. Disposal of this product, process solutions, residues and by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any local authority requirements. Dispose of surplus products and those that cannot be recycled via a licensed waste disposal contractor. Waste packaging should be collected for reuse or recycling. Incineration or landfill should only be considered when recycling is not feasible. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of the local water authority. SECTION 14: Transport information General The product is not covered by international regulations on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) No transport warning sign required Packing group Environmental hazards Environmentally hazardous substance/marine pollutant No Special precautions for user Transport in bulk according to Annex II of MARPOL73/78 and the IBC Code 7/8
16 Revision date: 12/08/2015 Elution Buffer Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information Safety, health and environmental regulations/legislation specific for the substance or mixture National regulations Health and Safety at Work etc. Act 1974 (as amended). The Carriage of Dangerous Goods and Use of Transportable Pressure Equipment Regulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"]. EH40/2005 Workplace exposure limits. EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (as amended). Commission Regulation (EU) No 453/2010 of 20 May Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures (as amended) Chemical safety assessment No chemical safety assessment has been carried out. SECTION 16: Other information Training advice Revision comments This product is considered to be a small package and is labelled according to the relevant provisions of the legislation. Read and follow manufacturer's recommendations. Only trained personnel should use this material. This is first issue. Revision date 12/08/2015 SDS number 3593 This information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process. Such information is, to the best of the company's knowledge and belief, accurate and reliable as of the date indicated. However, no warranty, guarantee or representation is made to its accuracy, reliability or completeness. It is the user's responsibility to satisfy himself as to the suitability of such information for his own particular use. 8/8
17 Revision Date 23/05/2014 SAFETY DATA SHEET Adapter Mix According to Regulation (EU) No 453/2010 SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING 1.1. Product identifier Product name Container size Adapter Mix 25 μl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Laboratory Reagent. For research purposes only Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA UK T: +44 (0) F: +44 (0) W: E: support@nanoporetech.com 1.4. Emergency telephone number +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: HAZARDS IDENTIFICATION 2.1. Classification of the substance or mixture Classification (EC 1272/2008) Physical and Chemical Hazards Not classified. Human health Not classified. Environment Not classified. Classification (1999/45/EEC) Not classified. The Full Text for all R-Phrases and Hazard Statements are Displayed in Section Label elements Label In Accordance With (EC) No. 1272/2008 No pictogram required. Precautionary Statements 2.3. Other hazards P280 Wear protective gloves, eye and face protection. This product does not contain any PBT or vpvb substances. SECTION 3: COMPOSITION/INFORMATION ON INGREDIENTS 3.2. Mixtures Composition Comments None of the ingredients are required to be listed. This product does not contain any hazardous ingredients, or ingredients with national workplace exposure limits. SECTION 4: FIRST AID MEASURES 1 / 6
18 Adapter Mix 4.1. Description of first aid measures Caution - substance not yet fully tested. Inhalation Move the exposed person to fresh air at once. Provide rest, warmth and fresh air. Get medical attention if any discomfort continues. Ingestion Do not induce vomiting. Rinse mouth thoroughly. Get medical attention if any discomfort continues. Skin contact Wash skin thoroughly with soap and water. Get medical attention promptly if symptoms occur after washing. Eye contact Make sure to remove any contact lenses from the eyes before rinsing. Immediately rinse with water. Get medical attention if any discomfort continues Most important symptoms and effects, both acute and delayed Inhalation No specific symptoms noted. Ingestion May cause discomfort if swallowed. Skin contact Prolonged skin contact may cause redness and irritation. Eye contact Prolonged contact may cause redness and/or tearing Indication of any immediate medical attention and special treatment needed Treat Symptomatically. SECTION 5: FIREFIGHTING MEASURES 5.1. Extinguishing media Extinguishing media Extinguish with foam, carbon dioxide, dry powder or water fog. Unsuitable extinguishing media Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Hazardous combustion products During fire, toxic gases (CO, CO2) are formed. Due to the small packaging, the risk of inhaling gases formed in a fire is minimal. Unusual Fire & Explosion Hazards No unusual fire or explosion hazards noted. Specific hazards No specific precautions due to the small quantities handled Advice for firefighters Special Fire Fighting Procedures Avoid breathing fire vapours. If possible, fight fire from protected position. Protective equipment for fire-fighters Self contained breathing apparatus and full protective clothing must be worn in case of fire. SECTION 6: ACCIDENTAL RELEASE MEASURES 6.1. Personal precautions, protective equipment and emergency procedures For personal protection, see section Environmental precautions Avoid discharge into drains, water courses or onto the ground Methods and material for containment and cleaning up Wear necessary protective equipment. Absorb spillage with suitable absorbent material. The product should not be dumped in nature but collected and delivered according to agreement with the local authorities Reference to other sections 2 / 6
19 Adapter Mix For personal protection, see section 8. See section 11 for additional information on health hazards. For waste disposal, see section 13. SECTION 7: HANDLING AND STORAGE 7.1. Precautions for safe handling Read and follow manufacturer's recommendations. Avoid spilling, skin and eye contact. Good personal hygiene is necessary. Wash hands and contaminated areas with water and soap before leaving the work site Conditions for safe storage, including any incompatibilities Store in tightly closed original container. Store at temperatures not exceeding 4 C Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: EXPOSURE CONTROLS/PERSONAL PROTECTION 8.1. Control parameters Ingredient Comments No exposure limits noted for ingredient(s) Exposure controls Engineering measures Provide adequate ventilation. Respiratory equipment No specific recommendations. Hand protection For prolonged or repeated skin contact use suitable protective gloves. The most suitable glove must be chosen in consultation with the gloves supplier, who can inform about the breakthrough time of the glove material. Eye protection Wear approved safety goggles. Provide eyewash station. Hygiene measures Wash hands after handling. When using do not eat, drink or smoke. Wash hands at the end of each work shift and before eating, smoking and using the toilet. Skin protection Wear apron or protective clothing in case of splashes. Environmental Exposure Controls Keep container tightly sealed when not in use. SECTION 9: PHYSICAL AND CHEMICAL PROPERTIES 9.1. Information on basic physical and chemical properties Appearance Liquid Colour Colourless. Odour No characteristic odour. Solubility Soluble in water. Initial boiling point and boiling range ~100 C ( C) Melting point ( C) ~ 0 C Relative density Bulk Density Vapour density (air=1) Vapour pressure Evaporation rate ph-value, Conc. Solution 3 / 6
20 Viscosity Decomposition temperature ( C) Odour Threshold, Lower Odour Threshold, Upper Flash point ( C) Auto Ignition Temperature ( C) Flammability Limit - Lower(%) Flammability Limit - Upper(%) Partition Coefficient (N-Octanol/Water) Explosive properties Not considered to be explosive. Oxidising properties Does not meet the criteria for oxidising. Adapter Mix 9.2. Other information No information required. SECTION 10: STABILITY AND REACTIVITY Reactivity No specific reactivity hazards associated with this product Chemical stability Stable under the prescribed storage conditions Possibility of hazardous reactions Not known Conditions to avoid Avoid excessive heat for prolonged periods of time Incompatible materials Materials To Avoid Strong oxidising substances. Strong reducing agents Hazardous decomposition products None at ambient temperatures. SECTION 11: TOXICOLOGICAL INFORMATION Information on toxicological effects Acute toxicity: Skin Corrosion/Irritation: Serious eye damage/irritation: Respiratory or skin sensitisation: 4 / 6
21 Adapter Mix Germ cell mutagenicity: Carcinogenicity: Reproductive Toxicity: Specific target organ toxicity - single exposure: Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure: Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard: SECTION 12: ECOLOGICAL INFORMATION Ecotoxicity The product components are not classified as environmentally hazardous. However, this does not exclude the possibility that large or frequent spills can have a harmful or damaging effect on the environment Toxicity Acute Fish Toxicity Not considered toxic to fish Persistence and degradability Degradability The degradability of the product has not been stated Bioaccumulative potential Bioaccumulative potential No data available on bioaccumulation. Partition coefficient Mobility in soil Mobility: The product is soluble in water Results of PBT and vpvb assessment This product does not contain any PBT or vpvb substances Other adverse effects None known. SECTION 13: DISPOSAL CONSIDERATIONS Avoid release to the environment Waste treatment methods Dispose of waste and residues in accordance with local authority requirements. SECTION 14: TRANSPORT INFORMATION General The product is not covered by international regulation on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number 5 / 6
22 Adapter Mix UN proper shipping name Transport hazard class(es) Transport Labels No transport warning sign required Packing group Environmental hazards Environmentally Hazardous Substance/Marine Pollutant No Special precautions for user Transport in bulk according to Annex II of MARPOL73/78 and the IBC Code SECTION 15: REGULATORY INFORMATION Safety, health and environmental regulations/legislation specific for the substance or mixture Approved Code Of Practice Classification and Labelling of Substances and Preparations Dangerous for Supply. Safety Data Sheets for Substances and Preparations. Guidance Notes Workplace Exposure Limits EH40. EU Legislation Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures, amending and repealing Directives 67/548/EEC and 1999/45/EC, and amending Regulation (EC) No 1907/2006 with amendments. Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH), establishing a European Chemicals Agency, amending Directive 1999/45/EC and repealing Council Regulation (EEC) No 793/93 and Commission Regulation (EC) No 1488/94 as well as Council Directive 76/769/EEC and Commission Directives 91/155/EEC, 93/67/EEC, 93/105/EC and 2000/21/EC, including amendments Chemical Safety Assessment No chemical safety assessment has been carried out. SECTION 16: OTHER INFORMATION Revision Comments This is first issue. Revision Date 23/05/2014 Risk Phrases In Full NC Not classified. 6 / 6
23 Revision date: 12/08/2015 SAFETY DATA SHEET HP Tether According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product name Container size HP Tether 10μl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Laboratory reagent. No specific uses advised against are identified Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA, UK T: +44 (0) F: +44 (0) E: support@nanoporetech.com 1.4. Emergency telephone number Emergency telephone +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification Physical hazards Not Classified Health hazards Environmental hazards Not Classified Not Classified 2.2. Label elements Hazard statements Precautionary statements Important Notes. NC Not Classified P280 Wear protective clothing, gloves, eye and face protection. This product is considered to be a small package and is labelled according to the relevant provisions of the legislation Other hazards This product does not contain any substances classified as PBT or vpvb. SECTION 3: Composition/information on ingredients 3.2. Mixtures 1/8
24 Revision date: 12/08/2015 HP Tether Composition comments No classified ingredients, or those having occupational exposure limits, present above the levels of disclosure.,this product contains a substance that has not yet been fully tested and may have unforeseen effects. SECTION 4: First aid measures 4.1. Description of first aid measures Inhalation Ingestion Skin contact Eye contact Protection of first aiders Not considered to be a significant hazard due to the small quantities used. Get medical attention if any discomfort continues. Show this Safety Data Sheet to the medical personnel. Move affected person to fresh air and keep warm and at rest in a position comfortable for breathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Rinse mouth thoroughly with water. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Give a few small glasses of water or milk to drink. Stop if the affected person feels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction of medical personnel. Remove affected person from source of contamination. Rinse immediately with plenty of water. Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wide apart. Continue to rinse for at least 10 minutes. First aid personnel should wear appropriate protective equipment during any rescue Most important symptoms and effects, both acute and delayed Inhalation Ingestion Skin contact Eye contact See Section 11 for additional information on health hazards. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Not considered to be a significant hazard due to the small quantities used. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed Notes for the doctor Specific treatments Treat symptomatically. No special treatment required. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire-extinguishing media suitable for the surrounding fire. Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Specific hazards Hazardous combustion products Containers can burst violently or explode when heated, due to excessive pressure build-up. Not considered to be a significant hazard due to the small quantities used. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours Advice for firefighters 2/8
25 Revision date: 12/08/2015 HP Tether Protective actions during firefighting Special protective equipment for firefighters Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat with water spray and remove them from the fire area if it can be done without risk. Cool containers exposed to flames with water until well after the fire is out. If a leak or spill has not ignited, use water spray to disperse vapours and protect men stopping the leak. Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protective clothing. Firefighter's clothing conforming to European standard EN469 (including helmets, protective boots and gloves) will provide a basic level of protection for chemical incidents. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keep unnecessary and unprotected personnel away from the spillage. Wear protective clothing as described in Section 8 of this safety data sheet. Follow precautions for safe handling described in this safety data sheet. Wash thoroughly after dealing with a spillage Environmental precautions Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities if environmental pollution occurs (sewers, waterways, soil or air) Methods and material for containment and cleaning up Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycle products wherever possible. If the product is soluble in water, dilute the spillage with water and mop it up. Flush contaminated area with plenty of water. Wash thoroughly after dealing with a spillage. Dispose of waste to licensed waste disposal site in accordance with the requirements of the local Waste Disposal Authority Reference to other sections Reference to other sections For personal protection, see Section 8. SECTION 7: Handling and storage 7.1. Precautions for safe handling Usage precautions Advice on general occupational hygiene Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs. Handle all packages and containers carefully to minimise spills. Keep container tightly sealed when not in use. Avoid the formation of mists. Wash promptly if skin becomes contaminated. Take off contaminated clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Wash at the end of each work shift and before eating, smoking and using the toilet. Change work clothing daily before leaving workplace Conditions for safe storage, including any incompatibilities Storage precautions Storage class Store in tightly-closed, original container in a dry, cool and well-ventilated place. Store at temperatures not exceeding 4 C. Unspecified storage Specific end use(s) Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: Exposure Controls/personal protection 8.1. Control parameters Ingredient comments No exposure limits known for ingredient(s). 3/8
26 Revision date: 12/08/2015 HP Tether 8.2. Exposure controls Protective equipment Appropriate engineering controls Eye/face protection Hand protection Other skin and body protection Hygiene measures Respiratory protection Environmental exposure controls Provide adequate ventilation. Good general ventilation should be adequate to control worker exposure to airborne contaminants. Eyewear complying with an approved standard should be worn if a risk assessment indicates eye contact is possible. Personal protective equipment for eye and face protection should comply with European Standard EN166. The following protection should be worn: Chemical splash goggles. Chemical-resistant, impervious gloves complying with an approved standard should be worn if a risk assessment indicates skin contact is possible. Considering the data specified by the glove manufacturer, check during use that the gloves are retaining their protective properties and change them as soon as any deterioration is detected. Appropriate footwear and additional protective clothing complying with an approved standard should be worn if a risk assessment indicates skin contamination is possible. Provide eyewash station and safety shower. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reuse. Clean equipment and the work area every day. Good personal hygiene procedures should be implemented. Wash at the end of each work shift and before eating, smoking and using the toilet. When using do not eat, drink or smoke. Respiratory protection complying with an approved standard should be worn if a risk assessment indicates inhalation of contaminants is possible. Provide adequate ventilation. Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn. Not regarded as dangerous for the environment. SECTION 9: Physical and Chemical Properties 9.1. Information on basic physical and chemical properties Appearance Colour Odour Odour threshold ph Melting point Initial boiling point and range Flash point Evaporation rate Upper/lower flammability or explosive limits Vapour pressure Vapour density Liquid. Colourless. Odourless. ~0 760 mm Hg ~ mm Hg 4/8
27 Revision date: 12/08/2015 HP Tether Relative density Solubility(ies) Partition coefficient Auto-ignition temperature Decomposition Temperature Viscosity Explosive properties Oxidising properties Soluble in water. Not considered to be explosive. Does not meet the criteria for classification as oxidising Other information Other information No information required. SECTION 10: Stability and reactivity Reactivity Reactivity There are no known reactivity hazards associated with this product Chemical stability Stability Stable at normal ambient temperatures and when used as recommended. Stable under the prescribed storage conditions Possibility of hazardous reactions Possibility of hazardous reactions No potentially hazardous reactions known Conditions to avoid Conditions to avoid Avoid excessive heat for prolonged periods of time Incompatible materials Materials to avoid Strong oxidising agents. Strong reducing agents Hazardous decomposition products Hazardous decomposition products Does not decompose when used and stored as recommended. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours. SECTION 11: Toxicological information Information on toxicological effects Toxicological effects Acute toxicity - oral Notes (oral LD₅₀) Acute toxicity - dermal Notes (dermal LD₅₀) Acute toxicity - inhalation Notes (inhalation LC₅₀) Skin corrosion/irritation Animal data Not regarded as a health hazard under current legislation. Serious eye damage/irritation 5/8
28 Revision date: 12/08/2015 HP Tether Serious eye damage/irritation Respiratory sensitisation Respiratory sensitisation Skin sensitisation Skin sensitisation Germ cell mutagenicity Genotoxicity - in vitro Carcinogenicity Carcinogenicity IARC carcinogenicity Reproductive toxicity Reproductive toxicity - fertility Reproductive toxicity - development None of the ingredients are listed or exempt. Specific target organ toxicity - single exposure STOT - single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure STOT - repeated exposure Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard Aspiration hazard Inhalation Ingestion Skin contact Eye contact Route of entry Target organs No specific health hazards known. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation. Ingestion Inhalation Skin and/or eye contact No specific target organs known. SECTION 12: Ecological Information Ecotoxicity Not considered to be a significant hazard due to the small quantities used. Not regarded as dangerous for the environment. However, large or frequent spills may have hazardous effects on the environment Toxicity Toxicity Persistence and degradability Persistence and degradability The degradability of the product is not known Bioaccumulative potential 6/8
29 Revision date: 12/08/2015 HP Tether Bioaccumulative potential Partition coefficient No data available on bioaccumulation Mobility in soil Mobility The product is water-soluble and may spread in water systems Results of PBT and vpvb assessment Results of PBT and vpvb assessment This product does not contain any substances classified as PBT or vpvb Other adverse effects Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Disposal methods The generation of waste should be minimised or avoided wherever possible. Reuse or recycle products wherever possible. This material and its container must be disposed of in a safe way. Disposal of this product, process solutions, residues and by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any local authority requirements. Dispose of surplus products and those that cannot be recycled via a licensed waste disposal contractor. Waste packaging should be collected for reuse or recycling. Incineration or landfill should only be considered when recycling is not feasible. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of the local water authority. SECTION 14: Transport information General The product is not covered by international regulations on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) No transport warning sign required Packing group Environmental hazards Environmentally hazardous substance/marine pollutant No Special precautions for user Transport in bulk according to Annex II of MARPOL73/78 and the IBC Code 7/8
30 Revision date: 12/08/2015 HP Tether Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information Safety, health and environmental regulations/legislation specific for the substance or mixture National regulations Health and Safety at Work etc. Act 1974 (as amended). The Carriage of Dangerous Goods and Use of Transportable Pressure Equipment Regulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"]. EH40/2005 Workplace exposure limits. EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (as amended). Commission Regulation (EU) No 453/2010 of 20 May Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures (as amended) Chemical safety assessment No chemical safety assessment has been carried out. SECTION 16: Other information Training advice Revision comments This product is considered to be a small package and is labelled according to the relevant provisions of the legislation. Read and follow manufacturer's recommendations. Only trained personnel should use this material. This is first issue. Revision date 12/08/2015 SDS number 3591 This information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process. Such information is, to the best of the company's knowledge and belief, accurate and reliable as of the date indicated. However, no warranty, guarantee or representation is made to its accuracy, reliability or completeness. It is the user's responsibility to satisfy himself as to the suitability of such information for his own particular use. 8/8
31 Revision date: 03/03/2016 SAFETY DATA SHEET Running Buffer FM1 According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product name Running Buffer FM Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Laboratory reagent. No specific uses advised against are identified Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA, UK T: +44 (0) F: +44 (0) E: support@nanoporetech.com 1.4. Emergency telephone number Emergency telephone +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification (EC/1272/2008) Physical hazards Not Classified Health hazards Environmental hazards Not Classified Not Classified 2.2. Label elements Hazard statements Precautionary statements Important Notes. NC Not Classified P280 Wear protective gloves/ protective clothing/ eye protection/ face protection. This product is considered to be a small package and is labelled according to the relevant provisions of the legislation Other hazards This product does not contain any substances classified as PBT or vpvb. SECTION 3: Composition/information on ingredients 3.2. Mixtures 1/8
32 Revision date: 03/03/2016 Running Buffer FM1 Composition comments This product does not contain any hazardous ingredients, or ingredients with national workplace exposure limits. SECTION 4: First aid measures 4.1. Description of first aid measures Inhalation Ingestion Skin contact Eye contact Protection of first aiders Not considered to be a significant hazard due to the small quantities used. Get medical attention if any discomfort continues. Show this Safety Data Sheet to the medical personnel. Move affected person to fresh air and keep warm and at rest in a position comfortable for breathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop if the affected person feels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction of medical personnel. Remove affected person from source of contamination. Rinse immediately with plenty of water. Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wide apart. Continue to rinse for at least 10 minutes. First aid personnel should wear appropriate protective equipment during any rescue Most important symptoms and effects, both acute and delayed Inhalation Ingestion Skin contact Eye contact See Section 11 for additional information on health hazards. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Not considered to be a significant hazard due to the small quantities used. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed Notes for the doctor Specific treatments Treat symptomatically. No special treatment required. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire-extinguishing media suitable for the surrounding fire. Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Specific hazards Hazardous combustion products Containers can burst violently or explode when heated, due to excessive pressure build-up. Not considered to be a significant hazard due to the small quantities used. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours Advice for firefighters 2/8
33 Revision date: 03/03/2016 Running Buffer FM1 Protective actions during firefighting Special protective equipment for firefighters Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat with water spray and remove them from the fire area if it can be done without risk. Cool containers exposed to flames with water until well after the fire is out. If a leak or spill has not ignited, use water spray to disperse vapours and protect men stopping the leak. Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protective clothing. Firefighter's clothing conforming to European standard EN469 (including helmets, protective boots and gloves) will provide a basic level of protection for chemical incidents. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Personal precautions Keep unnecessary and unprotected personnel away from the spillage. Wear protective clothing as described in Section 8 of this safety data sheet. Wash thoroughly after dealing with a spillage Environmental precautions Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities if environmental pollution occurs (sewers, waterways, soil or air) Methods and material for containment and cleaning up Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Clear up spills immediately and dispose of waste safely. Reuse or recycle products wherever possible. If the product is soluble in water, dilute the spillage with water and mop it up. Dispose of waste to licensed waste disposal site in accordance with the requirements of the local Waste Disposal Authority. Wash thoroughly after dealing with a spillage Reference to other sections Reference to other sections For personal protection, see Section 8. SECTION 7: Handling and storage 7.1. Precautions for safe handling Usage precautions Advice on general occupational hygiene Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs. Handle all packages and containers carefully to minimise spills. Keep container tightly sealed when not in use. Wash promptly if skin becomes contaminated. Take off contaminated clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Wash at the end of each work shift and before eating, smoking and using the toilet. Change work clothing daily before leaving workplace Conditions for safe storage, including any incompatibilities Storage precautions Storage class Store in accordance with local regulations. Unspecified storage Specific end use(s) Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: Exposure Controls/personal protection 8.1. Control parameters Ingredient comments No exposure limits known for ingredient(s) Exposure controls 3/8
34 Revision date: 03/03/2016 Running Buffer FM1 Protective equipment Appropriate engineering controls Eye/face protection Hand protection Other skin and body protection Hygiene measures Respiratory protection Environmental exposure controls Provide adequate ventilation. Good general ventilation should be adequate to control worker exposure to airborne contaminants. Eyewear complying with an approved standard should be worn if a risk assessment indicates eye contact is possible. Personal protective equipment for eye and face protection should comply with European Standard EN166. The following protection should be worn: Chemical splash goggles. Chemical-resistant, impervious gloves complying with an approved standard should be worn if a risk assessment indicates skin contact is possible. The most suitable glove should be chosen in consultation with the glove supplier/manufacturer, who can provide information about the breakthrough time of the glove material. To protect hands from chemicals, gloves should comply with European Standard EN374. Considering the data specified by the glove manufacturer, check during use that the gloves are retaining their protective properties and change them as soon as any deterioration is detected. Appropriate footwear and additional protective clothing complying with an approved standard should be worn if a risk assessment indicates skin contamination is possible. Provide eyewash station and safety shower. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reuse. Clean equipment and the work area every day. Good personal hygiene procedures should be implemented. Wash at the end of each work shift and before eating, smoking and using the toilet. When using do not eat, drink or smoke. Respiratory protection complying with an approved standard should be worn if a risk assessment indicates inhalation of contaminants is possible. Provide adequate ventilation. Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn. Not regarded as dangerous for the environment. SECTION 9: Physical and Chemical Properties 9.1. Information on basic physical and chemical properties Appearance Colour Odour Odour threshold ph Melting point Initial boiling point and range Flash point Evaporation rate Upper/lower flammability or explosive limits Vapour pressure Liquid. Colourless. Odourless. ~0 760 mm Hg ~ mm Hg 4/8
35 Revision date: 03/03/2016 Running Buffer FM1 Vapour density Relative density Solubility(ies) Partition coefficient Auto-ignition temperature Decomposition Temperature Viscosity Explosive properties Oxidising properties Soluble in water. Not considered to be explosive. Does not meet the criteria for classification as oxidising Other information Other information No information required. SECTION 10: Stability and reactivity Reactivity Reactivity There are no known reactivity hazards associated with this product Chemical stability Stability Stable at normal ambient temperatures and when used as recommended. Stable under the prescribed storage conditions Possibility of hazardous reactions Possibility of hazardous reactions No potentially hazardous reactions known Conditions to avoid Conditions to avoid There are no known conditions that are likely to result in a hazardous situation Incompatible materials Materials to avoid No specific material or group of materials is likely to react with the product to produce a hazardous situation Hazardous decomposition products Hazardous decomposition products Does not decompose when used and stored as recommended. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours. SECTION 11: Toxicological information Information on toxicological effects Toxicological effects Acute toxicity - oral Notes (oral LD₅₀) Acute toxicity - dermal Notes (dermal LD₅₀) Acute toxicity - inhalation Notes (inhalation LC₅₀) Not regarded as a health hazard under current legislation. Skin corrosion/irritation 5/8
36 Revision date: 03/03/2016 Running Buffer FM1 Animal data Serious eye damage/irritation Serious eye damage/irritation Respiratory sensitisation Respiratory sensitisation Skin sensitisation Skin sensitisation Germ cell mutagenicity Genotoxicity - in vitro Carcinogenicity Carcinogenicity IARC carcinogenicity Reproductive toxicity Reproductive toxicity - fertility Reproductive toxicity - development None of the ingredients are listed or exempt. Specific target organ toxicity - single exposure STOT - single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure STOT - repeated exposure Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard Aspiration hazard Inhalation Ingestion Skin contact Eye contact Route of entry Target organs No specific health hazards known. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation. Ingestion Inhalation Skin and/or eye contact No specific target organs known. SECTION 12: Ecological Information Ecotoxicity Not considered to be a significant hazard due to the small quantities used. Not regarded as dangerous for the environment. However, large or frequent spills may have hazardous effects on the environment Toxicity Toxicity Persistence and degradability Persistence and degradability The degradability of the product is not known. 6/8
37 Revision date: 03/03/2016 Running Buffer FM Bioaccumulative potential Bioaccumulative potential Partition coefficient No data available on bioaccumulation Mobility in soil Mobility The product is water-soluble and may spread in water systems Results of PBT and vpvb assessment Results of PBT and vpvb assessment This product does not contain any substances classified as PBT or vpvb Other adverse effects Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Disposal methods The generation of waste should be minimised or avoided wherever possible. Reuse or recycle products wherever possible. This material and its container must be disposed of in a safe way. Disposal of this product, process solutions, residues and by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any local authority requirements. Dispose of surplus products and those that cannot be recycled via a licensed waste disposal contractor. Waste packaging should be collected for reuse or recycling. Incineration or landfill should only be considered when recycling is not feasible. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of the local water authority. SECTION 14: Transport information General The product is not covered by international regulations on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) No transport warning sign required Packing group Environmental hazards Environmentally hazardous substance/marine pollutant No Special precautions for user Transport in bulk according to Annex II of MARPOL and the IBC Code 7/8
38 Revision date: 03/03/2016 Running Buffer FM1 Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information Safety, health and environmental regulations/legislation specific for the substance or mixture National regulations The Carriage of Dangerous Goods and Use of Transportable Pressure Equipment Regulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"]. EH40/2005 Workplace exposure limits. EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (as amended). Commission Regulation (EU) No 453/2010 of 20 May Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures (as amended) Chemical safety assessment No chemical safety assessment has been carried out. SECTION 16: Other information Training advice Revision comments This product is considered to be a small package and is labelled according to the relevant provisions of the legislation. Read and follow manufacturer's recommendations. This is first issue. Revision date 03/03/2016 SDS number 4225 This information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process. Such information is, to the best of the company's knowledge and belief, accurate and reliable as of the date indicated. However, no warranty, guarantee or representation is made to its accuracy, reliability or completeness. It is the user's responsibility to satisfy himself as to the suitability of such information for his own particular use. 8/8
39 SAFETY DATA SHEET DNA CS According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product name Container size DNA CS 50μl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Laboratory reagent. No specific uses advised against are identified Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA, UK T: +44 (0) F: +44 (0) E: support@nanoporetech.com 1.4. Emergency telephone number Emergency telephone +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification (EC/1272/2008) Physical hazards Not Classified Health hazards Environmental hazards Not Classified Not Classified 2.2. Label elements Hazard statements Precautionary statements Important Notes. NC Not Classified P280 Wear protective gloves/ protective clothing/ eye protection/ face protection. This product is considered to be a small package and is labelled according to the relevant provisions of the legislation Other hazards This product does not contain any substances classified as PBT or vpvb. SECTION 3: Composition/information on ingredients 3.2. Mixtures 1/8
40 DNA CS Composition comments This product does not contain any hazardous ingredients, or ingredients with national workplace exposure limits. SECTION 4: First aid measures 4.1. Description of first aid measures Inhalation Ingestion Skin contact Eye contact Protection of first aiders Not considered to be a significant hazard due to the small quantities used. Get medical attention if any discomfort continues. Show this Safety Data Sheet to the medical personnel. Move affected person to fresh air and keep warm and at rest in a position comfortable for breathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop if the affected person feels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction of medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Never give anything by mouth to an unconscious person. Remove affected person from source of contamination. Rinse immediately with plenty of water. Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wide apart. Continue to rinse for at least 10 minutes. First aid personnel should wear appropriate protective equipment during any rescue Most important symptoms and effects, both acute and delayed Inhalation Ingestion Skin contact Eye contact See Section 11 for additional information on health hazards. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Not considered to be a significant hazard due to the small quantities used. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed Notes for the doctor Specific treatments Treat symptomatically. No special treatment required. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire-extinguishing media suitable for the surrounding fire. Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Specific hazards Hazardous combustion products Containers can burst violently or explode when heated, due to excessive pressure build-up. Not considered to be a significant hazard due to the small quantities used. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours Advice for firefighters 2/8
41 DNA CS Protective actions during firefighting Special protective equipment for firefighters Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat with water spray and remove them from the fire area if it can be done without risk. Cool containers exposed to flames with water until well after the fire is out. If a leak or spill has not ignited, use water spray to disperse vapours and protect men stopping the leak. Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protective clothing. Firefighter's clothing conforming to European standard EN469 (including helmets, protective boots and gloves) will provide a basic level of protection for chemical incidents. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keep unnecessary and unprotected personnel away from the spillage. Wear protective clothing as described in Section 8 of this safety data sheet. Wash thoroughly after dealing with a spillage Environmental precautions Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities if environmental pollution occurs (sewers, waterways, soil or air) Methods and material for containment and cleaning up Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycle products wherever possible. If the product is soluble in water, dilute the spillage with water and mop it up. Flush contaminated area with plenty of water. Dispose of waste to licensed waste disposal site in accordance with the requirements of the local Waste Disposal Authority. Wash thoroughly after dealing with a spillage Reference to other sections Reference to other sections For personal protection, see Section 8. SECTION 7: Handling and storage 7.1. Precautions for safe handling Usage precautions Advice on general occupational hygiene Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs. Handle all packages and containers carefully to minimise spills. Keep container tightly sealed when not in use. Avoid the formation of mists. Wash promptly if skin becomes contaminated. Take off contaminated clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Wash at the end of each work shift and before eating, smoking and using the toilet. Change work clothing daily before leaving workplace Conditions for safe storage, including any incompatibilities Storage precautions Storage class Store in accordance with local regulations. Unspecified storage Specific end use(s) Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: Exposure Controls/personal protection 8.1. Control parameters Ingredient comments No exposure limits known for ingredient(s) Exposure controls 3/8
42 DNA CS Protective equipment Appropriate engineering controls Eye/face protection Hand protection Other skin and body protection Hygiene measures Respiratory protection Environmental exposure controls Provide adequate ventilation. Good general ventilation should be adequate to control worker exposure to airborne contaminants. Eyewear complying with an approved standard should be worn if a risk assessment indicates eye contact is possible. Personal protective equipment for eye and face protection should comply with European Standard EN166. The following protection should be worn: Chemical splash goggles. Chemical-resistant, impervious gloves complying with an approved standard should be worn if a risk assessment indicates skin contact is possible. The most suitable glove should be chosen in consultation with the glove supplier/manufacturer, who can provide information about the breakthrough time of the glove material. To protect hands from chemicals, gloves should comply with European Standard EN374. Considering the data specified by the glove manufacturer, check during use that the gloves are retaining their protective properties and change them as soon as any deterioration is detected. Frequent changes are recommended. Appropriate footwear and additional protective clothing complying with an approved standard should be worn if a risk assessment indicates skin contamination is possible. Provide eyewash station and safety shower. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reuse. Clean equipment and the work area every day. Good personal hygiene procedures should be implemented. Wash at the end of each work shift and before eating, smoking and using the toilet. When using do not eat, drink or smoke. Respiratory protection complying with an approved standard should be worn if a risk assessment indicates inhalation of contaminants is possible. Provide adequate ventilation. Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn. Not regarded as dangerous for the environment. SECTION 9: Physical and Chemical Properties 9.1. Information on basic physical and chemical properties Appearance Colour Odour Odour threshold ph Melting point Initial boiling point and range Flash point Evaporation rate Upper/lower flammability or explosive limits Vapour pressure Liquid. Colourless. Odourless. ~0 760 mm Hg ~ mm Hg 4/8
43 DNA CS Vapour density Relative density Solubility(ies) Partition coefficient Auto-ignition temperature Decomposition Temperature Viscosity Explosive properties Oxidising properties Soluble in water. Not considered to be explosive. Does not meet the criteria for classification as oxidising Other information Other information No information required. SECTION 10: Stability and reactivity Reactivity Reactivity There are no known reactivity hazards associated with this product Chemical stability Stability Stable at normal ambient temperatures and when used as recommended. Stable under the prescribed storage conditions Possibility of hazardous reactions Possibility of hazardous reactions No potentially hazardous reactions known Conditions to avoid Conditions to avoid There are no known conditions that are likely to result in a hazardous situation Incompatible materials Materials to avoid No specific material or group of materials is likely to react with the product to produce a hazardous situation Hazardous decomposition products Hazardous decomposition products Does not decompose when used and stored as recommended. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours. SECTION 11: Toxicological information Information on toxicological effects Toxicological effects Acute toxicity - oral Notes (oral LD₅₀) Acute toxicity - dermal Notes (dermal LD₅₀) Acute toxicity - inhalation Notes (inhalation LC₅₀) Not regarded as a health hazard under current legislation. Skin corrosion/irritation 5/8
44 DNA CS Animal data Serious eye damage/irritation Serious eye damage/irritation Respiratory sensitisation Respiratory sensitisation Skin sensitisation Skin sensitisation Germ cell mutagenicity Genotoxicity - in vitro Carcinogenicity Carcinogenicity IARC carcinogenicity Reproductive toxicity Reproductive toxicity - fertility Reproductive toxicity - development None of the ingredients are listed or exempt. Specific target organ toxicity - single exposure STOT - single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure STOT - repeated exposure Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard Aspiration hazard Inhalation Ingestion Skin contact Eye contact Route of entry Target organs No specific health hazards known. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation. Ingestion Inhalation Skin and/or eye contact No specific target organs known. SECTION 12: Ecological Information Ecotoxicity Not considered to be a significant hazard due to the small quantities used. Not regarded as dangerous for the environment. However, large or frequent spills may have hazardous effects on the environment Toxicity Toxicity Persistence and degradability 6/8
45 DNA CS Persistence and degradability The degradability of the product is not known Bioaccumulative potential Bioaccumulative potential Partition coefficient No data available on bioaccumulation Mobility in soil Mobility The product is water-soluble and may spread in water systems Results of PBT and vpvb assessment Results of PBT and vpvb assessment This product does not contain any substances classified as PBT or vpvb Other adverse effects Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Disposal methods The generation of waste should be minimised or avoided wherever possible. Reuse or recycle products wherever possible. This material and its container must be disposed of in a safe way. Disposal of this product, process solutions, residues and by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any local authority requirements. Dispose of surplus products and those that cannot be recycled via a licensed waste disposal contractor. Waste packaging should be collected for reuse or recycling. Incineration or landfill should only be considered when recycling is not feasible. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of the local water authority. SECTION 14: Transport information General The product is not covered by international regulations on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) No transport warning sign required Packing group Environmental hazards Environmentally hazardous substance/marine pollutant No Special precautions for user 7/8
46 DNA CS Transport in bulk according to Annex II of MARPOL and the IBC Code Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information Safety, health and environmental regulations/legislation specific for the substance or mixture National regulations The Carriage of Dangerous Goods and Use of Transportable Pressure Equipment Regulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"]. EH40/2005 Workplace exposure limits. EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (as amended). Commission Regulation (EU) No 453/2010 of 20 May Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures (as amended) Chemical safety assessment No chemical safety assessment has been carried out. SECTION 16: Other information Training advice Revision comments This product is considered to be a small package and is labelled according to the relevant provisions of the legislation. Read and follow manufacturer's recommendations. Only trained personnel should use this material. This is first issue. Revision date 10/03/2016 SDS number 4235 This information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process. Such information is, to the best of the company's knowledge and belief, accurate and reliable as of the date indicated. However, no warranty, guarantee or representation is made to its accuracy, reliability or completeness. It is the user's responsibility to satisfy himself as to the suitability of such information for his own particular use. 8/8
47 SAFETY DATA SHEET HP Adapter According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product name Container size HP Adapter 15μl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Laboratory reagent. No specific uses advised against are identified Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA, UK T: +44 (0) F: +44 (0) E: support@nanoporetech.com 1.4. Emergency telephone number Emergency telephone +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification (EC/1272/2008) Physical hazards Not Classified Health hazards Environmental hazards Not Classified Not Classified 2.2. Label elements Hazard statements Precautionary statements Important Notes. NC Not Classified P280 Wear protective gloves/ protective clothing/ eye protection/ face protection. This product is considered to be a small package and is labelled according to the relevant provisions of the legislation Other hazards This product does not contain any substances classified as PBT or vpvb. SECTION 3: Composition/information on ingredients 3.2. Mixtures 1/8
48 HP Adapter Composition comments This product does not contain any hazardous ingredients, or ingredients with national workplace exposure limits. SECTION 4: First aid measures 4.1. Description of first aid measures Inhalation Ingestion Skin contact Eye contact Protection of first aiders Not considered to be a significant hazard due to the small quantities used. Get medical attention if any discomfort continues. Show this Safety Data Sheet to the medical personnel. Move affected person to fresh air and keep warm and at rest in a position comfortable for breathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop if the affected person feels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction of medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Never give anything by mouth to an unconscious person. Remove affected person from source of contamination. Rinse immediately with plenty of water. Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wide apart. Continue to rinse for at least 10 minutes. First aid personnel should wear appropriate protective equipment during any rescue Most important symptoms and effects, both acute and delayed Inhalation Ingestion Skin contact Eye contact See Section 11 for additional information on health hazards. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Not considered to be a significant hazard due to the small quantities used. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed Notes for the doctor Specific treatments Treat symptomatically. No special treatment required. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire-extinguishing media suitable for the surrounding fire. Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Specific hazards Hazardous combustion products Containers can burst violently or explode when heated, due to excessive pressure build-up. Not considered to be a significant hazard due to the small quantities used. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours Advice for firefighters 2/8
49 HP Adapter Protective actions during firefighting Special protective equipment for firefighters Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat with water spray and remove them from the fire area if it can be done without risk. Cool containers exposed to flames with water until well after the fire is out. If a leak or spill has not ignited, use water spray to disperse vapours and protect men stopping the leak. Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protective clothing. Firefighter's clothing conforming to European standard EN469 (including helmets, protective boots and gloves) will provide a basic level of protection for chemical incidents. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keep unnecessary and unprotected personnel away from the spillage. Wear protective clothing as described in Section 8 of this safety data sheet. Wash thoroughly after dealing with a spillage Environmental precautions Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities if environmental pollution occurs (sewers, waterways, soil or air) Methods and material for containment and cleaning up Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycle products wherever possible. If the product is soluble in water, dilute the spillage with water and mop it up. Flush contaminated area with plenty of water. Dispose of contents/container in accordance with national regulations. Wash thoroughly after dealing with a spillage Reference to other sections Reference to other sections For personal protection, see Section 8. SECTION 7: Handling and storage 7.1. Precautions for safe handling Usage precautions Advice on general occupational hygiene Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs. Handle all packages and containers carefully to minimise spills. Keep container tightly sealed when not in use. Avoid the formation of mists. Wash promptly if skin becomes contaminated. Take off contaminated clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Wash at the end of each work shift and before eating, smoking and using the toilet. Change work clothing daily before leaving workplace Conditions for safe storage, including any incompatibilities Storage precautions Storage class Store in accordance with local regulations. Unspecified storage Specific end use(s) Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: Exposure Controls/personal protection 8.1. Control parameters Ingredient comments No exposure limits known for ingredient(s) Exposure controls 3/8
50 HP Adapter Protective equipment Appropriate engineering controls Eye/face protection Hand protection Other skin and body protection Hygiene measures Respiratory protection Environmental exposure controls Provide adequate ventilation. Good general ventilation should be adequate to control worker exposure to airborne contaminants. Eyewear complying with an approved standard should be worn if a risk assessment indicates eye contact is possible. Personal protective equipment for eye and face protection should comply with European Standard EN166. The following protection should be worn: Chemical splash goggles. Chemical-resistant, impervious gloves complying with an approved standard should be worn if a risk assessment indicates skin contact is possible. The most suitable glove should be chosen in consultation with the glove supplier/manufacturer, who can provide information about the breakthrough time of the glove material. To protect hands from chemicals, gloves should comply with European Standard EN374. Considering the data specified by the glove manufacturer, check during use that the gloves are retaining their protective properties and change them as soon as any deterioration is detected. Frequent changes are recommended. Appropriate footwear and additional protective clothing complying with an approved standard should be worn if a risk assessment indicates skin contamination is possible. Provide eyewash station and safety shower. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reuse. Clean equipment and the work area every day. Good personal hygiene procedures should be implemented. Wash at the end of each work shift and before eating, smoking and using the toilet. When using do not eat, drink or smoke. Respiratory protection complying with an approved standard should be worn if a risk assessment indicates inhalation of contaminants is possible. Provide adequate ventilation. Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn. Not regarded as dangerous for the environment. SECTION 9: Physical and Chemical Properties 9.1. Information on basic physical and chemical properties Appearance Colour Odour Odour threshold ph Melting point Initial boiling point and range Flash point Evaporation rate Upper/lower flammability or explosive limits Vapour pressure Liquid. Colourless. Odourless. ~0 760 mm Hg ~ mm Hg 4/8
51 HP Adapter Vapour density Relative density Solubility(ies) Partition coefficient Auto-ignition temperature Decomposition Temperature Viscosity Explosive properties Oxidising properties Soluble in water. Not considered to be explosive. Does not meet the criteria for classification as oxidising Other information Other information No information required. SECTION 10: Stability and reactivity Reactivity Reactivity There are no known reactivity hazards associated with this product Chemical stability Stability Stable at normal ambient temperatures and when used as recommended. Stable under the prescribed storage conditions Possibility of hazardous reactions Possibility of hazardous reactions No potentially hazardous reactions known Conditions to avoid Conditions to avoid There are no known conditions that are likely to result in a hazardous situation Incompatible materials Materials to avoid No specific material or group of materials is likely to react with the product to produce a hazardous situation Hazardous decomposition products Hazardous decomposition products Does not decompose when used and stored as recommended. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours. SECTION 11: Toxicological information Information on toxicological effects Toxicological effects Acute toxicity - oral Notes (oral LD₅₀) Acute toxicity - dermal Notes (dermal LD₅₀) Acute toxicity - inhalation Notes (inhalation LC₅₀) Not regarded as a health hazard under current legislation. Skin corrosion/irritation 5/8
52 HP Adapter Animal data Serious eye damage/irritation Serious eye damage/irritation Respiratory sensitisation Respiratory sensitisation Skin sensitisation Skin sensitisation Germ cell mutagenicity Genotoxicity - in vitro Carcinogenicity Carcinogenicity IARC carcinogenicity Reproductive toxicity Reproductive toxicity - fertility Reproductive toxicity - development None of the ingredients are listed or exempt. Specific target organ toxicity - single exposure STOT - single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure STOT - repeated exposure Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard Aspiration hazard Inhalation Ingestion Skin contact Eye contact Route of entry Target organs No specific health hazards known. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation. Ingestion Inhalation Skin and/or eye contact No specific target organs known. SECTION 12: Ecological Information Ecotoxicity Not considered to be a significant hazard due to the small quantities used. Not regarded as dangerous for the environment. However, large or frequent spills may have hazardous effects on the environment Toxicity Toxicity Persistence and degradability 6/8
53 HP Adapter Persistence and degradability The degradability of the product is not known Bioaccumulative potential Bioaccumulative potential Partition coefficient No data available on bioaccumulation Mobility in soil Mobility The product is water-soluble and may spread in water systems Results of PBT and vpvb assessment Results of PBT and vpvb assessment This product does not contain any substances classified as PBT or vpvb Other adverse effects Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Disposal methods The generation of waste should be minimised or avoided wherever possible. Reuse or recycle products wherever possible. This material and its container must be disposed of in a safe way. Disposal of this product, process solutions, residues and by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any local authority requirements. Dispose of surplus products and those that cannot be recycled via a licensed waste disposal contractor. Waste packaging should be collected for reuse or recycling. Incineration or landfill should only be considered when recycling is not feasible. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of the local water authority. SECTION 14: Transport information General The product is not covered by international regulations on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) No transport warning sign required Packing group Environmental hazards Environmentally hazardous substance/marine pollutant No Special precautions for user 7/8
54 HP Adapter Transport in bulk according to Annex II of MARPOL and the IBC Code Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information Safety, health and environmental regulations/legislation specific for the substance or mixture National regulations The Carriage of Dangerous Goods and Use of Transportable Pressure Equipment Regulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"]. EH40/2005 Workplace exposure limits. EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (as amended). Commission Regulation (EU) No 453/2010 of 20 May Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures (as amended) Chemical safety assessment No chemical safety assessment has been carried out. SECTION 16: Other information Training advice Revision comments This product is considered to be a small package and is labelled according to the relevant provisions of the legislation. Read and follow manufacturer's recommendations. Only trained personnel should use this material. This is first issue. Revision date 10/03/2016 SDS number 4236 This information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process. Such information is, to the best of the company's knowledge and belief, accurate and reliable as of the date indicated. However, no warranty, guarantee or representation is made to its accuracy, reliability or completeness. It is the user's responsibility to satisfy himself as to the suitability of such information for his own particular use. 8/8
55 SAFETY DATA SHEET Bead Binding Buffer According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product name Container size Bead Binding Buffer 2mL 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Laboratory reagent. No specific uses advised against are identified Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA, UK T: +44 (0) F: +44 (0) E: support@nanoporetech.com 1.4. Emergency telephone number Emergency telephone +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification (EC/1272/2008) Physical hazards Not Classified Health hazards Environmental hazards Not Classified Not Classified 2.2. Label elements Hazard statements Precautionary statements Important Notes. NC Not Classified P280 Wear protective gloves/ protective clothing/ eye protection/ face protection. This product is considered to be a small package and is labelled according to the relevant provisions of the legislation Other hazards This product does not contain any substances classified as PBT or vpvb. SECTION 3: Composition/information on ingredients 3.2. Mixtures 1/8
56 Bead Binding Buffer Composition comments This product does not contain any hazardous ingredients, or ingredients with national workplace exposure limits. SECTION 4: First aid measures 4.1. Description of first aid measures Inhalation Ingestion Skin contact Eye contact Protection of first aiders Not considered to be a significant hazard due to the small quantities used. Get medical attention if any discomfort continues. Show this Safety Data Sheet to the medical personnel. Move affected person to fresh air and keep warm and at rest in a position comfortable for breathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop if the affected person feels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction of medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Never give anything by mouth to an unconscious person. Remove affected person from source of contamination. Rinse immediately with plenty of water. Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wide apart. Continue to rinse for at least 10 minutes. First aid personnel should wear appropriate protective equipment during any rescue Most important symptoms and effects, both acute and delayed Inhalation Ingestion Skin contact Eye contact See Section 11 for additional information on health hazards. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Not considered to be a significant hazard due to the small quantities used. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed Notes for the doctor Specific treatments Treat symptomatically. No special treatment required. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire-extinguishing media suitable for the surrounding fire. Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Specific hazards Hazardous combustion products Containers can burst violently or explode when heated, due to excessive pressure build-up. Not considered to be a significant hazard due to the small quantities used. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours Advice for firefighters 2/8
57 Bead Binding Buffer Protective actions during firefighting Special protective equipment for firefighters Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat with water spray and remove them from the fire area if it can be done without risk. Cool containers exposed to flames with water until well after the fire is out. If a leak or spill has not ignited, use water spray to disperse vapours and protect men stopping the leak. Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protective clothing. Firefighter's clothing conforming to European standard EN469 (including helmets, protective boots and gloves) will provide a basic level of protection for chemical incidents. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keep unnecessary and unprotected personnel away from the spillage. Wear protective clothing as described in Section 8 of this safety data sheet. Wash thoroughly after dealing with a spillage Environmental precautions Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities if environmental pollution occurs (sewers, waterways, soil or air) Methods and material for containment and cleaning up Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycle products wherever possible. If the product is soluble in water, dilute the spillage with water and mop it up. Flush contaminated area with plenty of water. Dispose of waste to licensed waste disposal site in accordance with the requirements of the local Waste Disposal Authority. Wash thoroughly after dealing with a spillage Reference to other sections Reference to other sections For personal protection, see Section 8. SECTION 7: Handling and storage 7.1. Precautions for safe handling Usage precautions Advice on general occupational hygiene Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs. Handle all packages and containers carefully to minimise spills. Keep container tightly sealed when not in use. Avoid the formation of mists. Wash promptly if skin becomes contaminated. Take off contaminated clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Wash at the end of each work shift and before eating, smoking and using the toilet. Change work clothing daily before leaving workplace Conditions for safe storage, including any incompatibilities Storage precautions Storage class Store in accordance with local regulations. Unspecified storage Specific end use(s) Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: Exposure Controls/personal protection 8.1. Control parameters Ingredient comments No exposure limits known for ingredient(s) Exposure controls 3/8
58 Bead Binding Buffer Protective equipment Appropriate engineering controls Eye/face protection Hand protection Other skin and body protection Hygiene measures Respiratory protection Environmental exposure controls Provide adequate ventilation. Good general ventilation should be adequate to control worker exposure to airborne contaminants. Eyewear complying with an approved standard should be worn if a risk assessment indicates eye contact is possible. Personal protective equipment for eye and face protection should comply with European Standard EN166. The following protection should be worn: Chemical splash goggles. Chemical-resistant, impervious gloves complying with an approved standard should be worn if a risk assessment indicates skin contact is possible. The most suitable glove should be chosen in consultation with the glove supplier/manufacturer, who can provide information about the breakthrough time of the glove material. To protect hands from chemicals, gloves should comply with European Standard EN374. Considering the data specified by the glove manufacturer, check during use that the gloves are retaining their protective properties and change them as soon as any deterioration is detected. Frequent changes are recommended. Appropriate footwear and additional protective clothing complying with an approved standard should be worn if a risk assessment indicates skin contamination is possible. Provide eyewash station and safety shower. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reuse. Clean equipment and the work area every day. Good personal hygiene procedures should be implemented. Wash at the end of each work shift and before eating, smoking and using the toilet. When using do not eat, drink or smoke. Respiratory protection complying with an approved standard should be worn if a risk assessment indicates inhalation of contaminants is possible. Provide adequate ventilation. Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn. Not regarded as dangerous for the environment. SECTION 9: Physical and Chemical Properties 9.1. Information on basic physical and chemical properties Appearance Colour Odour Odour threshold ph Melting point Initial boiling point and range Flash point Evaporation rate Upper/lower flammability or explosive limits Vapour pressure Liquid. Colourless. Odourless. ~0 760 mm Hg ~ mm Hg 4/8
59 Bead Binding Buffer Vapour density Relative density Solubility(ies) Partition coefficient Auto-ignition temperature Decomposition Temperature Viscosity Explosive properties Oxidising properties Soluble in water. Not considered to be explosive. Does not meet the criteria for classification as oxidising Other information Other information No information required. SECTION 10: Stability and reactivity Reactivity Reactivity There are no known reactivity hazards associated with this product Chemical stability Stability Stable at normal ambient temperatures and when used as recommended. Stable under the prescribed storage conditions Possibility of hazardous reactions Possibility of hazardous reactions No potentially hazardous reactions known Conditions to avoid Conditions to avoid There are no known conditions that are likely to result in a hazardous situation Incompatible materials Materials to avoid No specific material or group of materials is likely to react with the product to produce a hazardous situation Hazardous decomposition products Hazardous decomposition products Does not decompose when used and stored as recommended. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours. SECTION 11: Toxicological information Information on toxicological effects Toxicological effects Acute toxicity - oral Notes (oral LD₅₀) Acute toxicity - dermal Notes (dermal LD₅₀) Acute toxicity - inhalation Notes (inhalation LC₅₀) Not regarded as a health hazard under current legislation. Skin corrosion/irritation 5/8
60 Bead Binding Buffer Animal data Serious eye damage/irritation Serious eye damage/irritation Respiratory sensitisation Respiratory sensitisation Skin sensitisation Skin sensitisation Germ cell mutagenicity Genotoxicity - in vitro Carcinogenicity Carcinogenicity IARC carcinogenicity Reproductive toxicity Reproductive toxicity - fertility Reproductive toxicity - development None of the ingredients are listed or exempt. Specific target organ toxicity - single exposure STOT - single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure STOT - repeated exposure Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard Aspiration hazard Inhalation Ingestion Skin contact Eye contact Route of entry Target organs No specific health hazards known. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation. Ingestion Inhalation Skin and/or eye contact No specific target organs known. SECTION 12: Ecological Information Ecotoxicity Not considered to be a significant hazard due to the small quantities used. Not regarded as dangerous for the environment. However, large or frequent spills may have hazardous effects on the environment Toxicity Toxicity Persistence and degradability 6/8
61 Bead Binding Buffer Persistence and degradability The degradability of the product is not known Bioaccumulative potential Bioaccumulative potential Partition coefficient No data available on bioaccumulation Mobility in soil Mobility The product is water-soluble and may spread in water systems Results of PBT and vpvb assessment Results of PBT and vpvb assessment This product does not contain any substances classified as PBT or vpvb Other adverse effects Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Disposal methods The generation of waste should be minimised or avoided wherever possible. Reuse or recycle products wherever possible. This material and its container must be disposed of in a safe way. Disposal of this product, process solutions, residues and by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any local authority requirements. Dispose of surplus products and those that cannot be recycled via a licensed waste disposal contractor. Waste packaging should be collected for reuse or recycling. Incineration or landfill should only be considered when recycling is not feasible. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of the local water authority. SECTION 14: Transport information General The product is not covered by international regulations on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) No transport warning sign required Packing group Environmental hazards Environmentally hazardous substance/marine pollutant No Special precautions for user 7/8
62 Bead Binding Buffer Transport in bulk according to Annex II of MARPOL and the IBC Code Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information Safety, health and environmental regulations/legislation specific for the substance or mixture National regulations The Carriage of Dangerous Goods and Use of Transportable Pressure Equipment Regulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"]. EH40/2005 Workplace exposure limits. EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (as amended). Commission Regulation (EU) No 453/2010 of 20 May Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures (as amended) Chemical safety assessment No chemical safety assessment has been carried out. SECTION 16: Other information Training advice Revision comments This product is considered to be a small package and is labelled according to the relevant provisions of the legislation. Read and follow manufacturer's recommendations. Only trained personnel should use this material. This is first issue. Revision date 10/03/2016 SDS number 4237 This information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process. Such information is, to the best of the company's knowledge and belief, accurate and reliable as of the date indicated. However, no warranty, guarantee or representation is made to its accuracy, reliability or completeness. It is the user's responsibility to satisfy himself as to the suitability of such information for his own particular use. 8/8
63 SAFETY DATA SHEET PCR Adapters According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product name Container size PCR Adapters 140μl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Laboratory reagent. No specific uses advised against are identified Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA, UK T: +44 (0) F: +44 (0) E: support@nanoporetech.com 1.4. Emergency telephone number Emergency telephone +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification (EC/1272/2008) Physical hazards Not Classified Health hazards Environmental hazards Not Classified Not Classified 2.2. Label elements Hazard statements Precautionary statements Important Notes. NC Not Classified P280 Wear protective gloves/ protective clothing/ eye protection/ face protection. This product is considered to be a small package and is labelled according to the relevant provisions of the legislation Other hazards This product does not contain any substances classified as PBT or vpvb. SECTION 3: Composition/information on ingredients 3.2. Mixtures 1/8
64 PCR Adapters Composition comments This product does not contain any hazardous ingredients, or ingredients with national workplace exposure limits. SECTION 4: First aid measures 4.1. Description of first aid measures Inhalation Ingestion Skin contact Eye contact Protection of first aiders Not considered to be a significant hazard due to the small quantities used. Get medical attention if any discomfort continues. Show this Safety Data Sheet to the medical personnel. Move affected person to fresh air and keep warm and at rest in a position comfortable for breathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop if the affected person feels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction of medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Never give anything by mouth to an unconscious person. Remove affected person from source of contamination. Rinse immediately with plenty of water. Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wide apart. Continue to rinse for at least 10 minutes. First aid personnel should wear appropriate protective equipment during any rescue Most important symptoms and effects, both acute and delayed Inhalation Ingestion Skin contact Eye contact See Section 11 for additional information on health hazards. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Not considered to be a significant hazard due to the small quantities used. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed Notes for the doctor Specific treatments Treat symptomatically. No special treatment required. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire-extinguishing media suitable for the surrounding fire. Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Specific hazards Hazardous combustion products Containers can burst violently or explode when heated, due to excessive pressure build-up. Not considered to be a significant hazard due to the small quantities used. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours Advice for firefighters 2/8
65 PCR Adapters Protective actions during firefighting Special protective equipment for firefighters Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat with water spray and remove them from the fire area if it can be done without risk. Cool containers exposed to flames with water until well after the fire is out. If a leak or spill has not ignited, use water spray to disperse vapours and protect men stopping the leak. Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protective clothing. Firefighter's clothing conforming to European standard EN469 (including helmets, protective boots and gloves) will provide a basic level of protection for chemical incidents. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keep unnecessary and unprotected personnel away from the spillage. Wear protective clothing as described in Section 8 of this safety data sheet. Wash thoroughly after dealing with a spillage Environmental precautions Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities if environmental pollution occurs (sewers, waterways, soil or air) Methods and material for containment and cleaning up Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycle products wherever possible. If the product is soluble in water, dilute the spillage with water and mop it up. Flush contaminated area with plenty of water. Dispose of waste to licensed waste disposal site in accordance with the requirements of the local Waste Disposal Authority. Wash thoroughly after dealing with a spillage Reference to other sections Reference to other sections For personal protection, see Section 8. SECTION 7: Handling and storage 7.1. Precautions for safe handling Usage precautions Advice on general occupational hygiene Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs. Handle all packages and containers carefully to minimise spills. Keep container tightly sealed when not in use. Avoid the formation of mists. Wash promptly if skin becomes contaminated. Take off contaminated clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Wash at the end of each work shift and before eating, smoking and using the toilet. Change work clothing daily before leaving workplace Conditions for safe storage, including any incompatibilities Storage precautions Storage class Store in accordance with local regulations. Unspecified storage Specific end use(s) Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: Exposure Controls/personal protection 8.1. Control parameters Ingredient comments No exposure limits known for ingredient(s) Exposure controls 3/8
66 PCR Adapters Protective equipment Appropriate engineering controls Eye/face protection Hand protection Other skin and body protection Hygiene measures Respiratory protection Environmental exposure controls Provide adequate ventilation. Good general ventilation should be adequate to control worker exposure to airborne contaminants. Eyewear complying with an approved standard should be worn if a risk assessment indicates eye contact is possible. Personal protective equipment for eye and face protection should comply with European Standard EN166. The following protection should be worn: Chemical splash goggles. Chemical-resistant, impervious gloves complying with an approved standard should be worn if a risk assessment indicates skin contact is possible. The most suitable glove should be chosen in consultation with the glove supplier/manufacturer, who can provide information about the breakthrough time of the glove material. To protect hands from chemicals, gloves should comply with European Standard EN374. Considering the data specified by the glove manufacturer, check during use that the gloves are retaining their protective properties and change them as soon as any deterioration is detected. Frequent changes are recommended. Appropriate footwear and additional protective clothing complying with an approved standard should be worn if a risk assessment indicates skin contamination is possible. Provide eyewash station and safety shower. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reuse. Clean equipment and the work area every day. Good personal hygiene procedures should be implemented. Wash at the end of each work shift and before eating, smoking and using the toilet. When using do not eat, drink or smoke. Respiratory protection complying with an approved standard should be worn if a risk assessment indicates inhalation of contaminants is possible. Provide adequate ventilation. Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn. Not regarded as dangerous for the environment. SECTION 9: Physical and Chemical Properties 9.1. Information on basic physical and chemical properties Appearance Colour Odour Odour threshold ph Melting point Initial boiling point and range Flash point Evaporation rate Upper/lower flammability or explosive limits Vapour pressure Liquid. Colourless. Odourless. ~0 760 mm Hg ~ mm Hg 4/8
67 PCR Adapters Vapour density Relative density Solubility(ies) Partition coefficient Auto-ignition temperature Decomposition Temperature Viscosity Explosive properties Oxidising properties Soluble in water. Not considered to be explosive. Does not meet the criteria for classification as oxidising Other information Other information No information required. SECTION 10: Stability and reactivity Reactivity Reactivity There are no known reactivity hazards associated with this product Chemical stability Stability Stable at normal ambient temperatures and when used as recommended. Stable under the prescribed storage conditions Possibility of hazardous reactions Possibility of hazardous reactions No potentially hazardous reactions known Conditions to avoid Conditions to avoid There are no known conditions that are likely to result in a hazardous situation Incompatible materials Materials to avoid No specific material or group of materials is likely to react with the product to produce a hazardous situation Hazardous decomposition products Hazardous decomposition products Does not decompose when used and stored as recommended. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours. SECTION 11: Toxicological information Information on toxicological effects Toxicological effects Acute toxicity - oral Notes (oral LD₅₀) Acute toxicity - dermal Notes (dermal LD₅₀) Acute toxicity - inhalation Notes (inhalation LC₅₀) Not regarded as a health hazard under current legislation. Skin corrosion/irritation 5/8
68 PCR Adapters Animal data Serious eye damage/irritation Serious eye damage/irritation Respiratory sensitisation Respiratory sensitisation Skin sensitisation Skin sensitisation Germ cell mutagenicity Genotoxicity - in vitro Carcinogenicity Carcinogenicity IARC carcinogenicity Reproductive toxicity Reproductive toxicity - fertility Reproductive toxicity - development None of the ingredients are listed or exempt. Specific target organ toxicity - single exposure STOT - single exposure Not classified as a specific target organ toxicant after a single exposure. Specific target organ toxicity - repeated exposure STOT - repeated exposure Not classified as a specific target organ toxicant after repeated exposure. Aspiration hazard Aspiration hazard Inhalation Ingestion Skin contact Eye contact Route of entry Target organs No specific health hazards known. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation. Ingestion Inhalation Skin and/or eye contact No specific target organs known. SECTION 12: Ecological Information Ecotoxicity Not considered to be a significant hazard due to the small quantities used. Not regarded as dangerous for the environment. However, large or frequent spills may have hazardous effects on the environment Toxicity Toxicity Persistence and degradability 6/8
69 PCR Adapters Persistence and degradability The degradability of the product is not known Bioaccumulative potential Bioaccumulative potential Partition coefficient No data available on bioaccumulation Mobility in soil Mobility The product is water-soluble and may spread in water systems Results of PBT and vpvb assessment Results of PBT and vpvb assessment This product does not contain any substances classified as PBT or vpvb Other adverse effects Other adverse effects None known. SECTION 13: Disposal considerations Waste treatment methods Disposal methods The generation of waste should be minimised or avoided wherever possible. Reuse or recycle products wherever possible. This material and its container must be disposed of in a safe way. Disposal of this product, process solutions, residues and by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any local authority requirements. Dispose of surplus products and those that cannot be recycled via a licensed waste disposal contractor. Waste packaging should be collected for reuse or recycling. Incineration or landfill should only be considered when recycling is not feasible. Waste should not be disposed of untreated to the sewer unless fully compliant with the requirements of the local water authority. SECTION 14: Transport information General The product is not covered by international regulations on the transport of dangerous goods (IMDG, IATA, ADR/RID) UN number UN proper shipping name Transport hazard class(es) No transport warning sign required Packing group Environmental hazards Environmentally hazardous substance/marine pollutant No Special precautions for user 7/8
70 PCR Adapters Transport in bulk according to Annex II of MARPOL and the IBC Code Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15: Regulatory information Safety, health and environmental regulations/legislation specific for the substance or mixture National regulations The Carriage of Dangerous Goods and Use of Transportable Pressure Equipment Regulations 2009 (SI 2009 No. 1348) (as amended) ["CDG 2009"]. EH40/2005 Workplace exposure limits. EU legislation Regulation (EC) No 1907/2006 of the European Parliament and of the Council of 18 December 2006 concerning the Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH) (as amended). Commission Regulation (EU) No 453/2010 of 20 May Regulation (EC) No 1272/2008 of the European Parliament and of the Council of 16 December 2008 on classification, labelling and packaging of substances and mixtures (as amended) Chemical safety assessment No chemical safety assessment has been carried out. SECTION 16: Other information Training advice Revision comments This product is considered to be a small package and is labelled according to the relevant provisions of the legislation. Read and follow manufacturer's recommendations. Only trained personnel should use this material. This is first issue. Revision date 10/03/2016 SDS number 4238 This information relates only to the specific material designated and may not be valid for such material used in combination with any other materials or in any process. Such information is, to the best of the company's knowledge and belief, accurate and reliable as of the date indicated. However, no warranty, guarantee or representation is made to its accuracy, reliability or completeness. It is the user's responsibility to satisfy himself as to the suitability of such information for his own particular use. 8/8
71 SAFETY DATA SHEET PCR Primers According to Regulation (EC) No 1907/2006, Annex II, as amended by Regulation (EU) No 453/2010 SECTION 1: Identification of the substance/mixture and of the company/undertaking 1.1. Product identifier Product name Container size PCR Primers 20μl 1.2. Relevant identified uses of the substance or mixture and uses advised against Identified uses Uses advised against Laboratory reagent. No specific uses advised against are identified Details of the supplier of the safety data sheet Supplier Oxford Nanopore Technologies Ltd. Edmund Cartwright House, 4 Robert Robinson Avenue, Oxford Science Park, Oxford, OX4 4GA, UK T: +44 (0) F: +44 (0) E: support@nanoporetech.com 1.4. Emergency telephone number Emergency telephone +44 (0) (Mon - Fri 09:00-17:00 h) SECTION 2: Hazards identification 2.1. Classification of the substance or mixture Classification (EC/1272/2008) Physical hazards Not Classified Health hazards Environmental hazards Not Classified Not Classified 2.2. Label elements Hazard statements Precautionary statements Important Notes. NC Not Classified P280 Wear protective gloves/ protective clothing/ eye protection/ face protection. This product is considered to be a small package and is labelled according to the relevant provisions of the legislation Other hazards This product does not contain any substances classified as PBT or vpvb. SECTION 3: Composition/information on ingredients 3.2. Mixtures 1/8
72 PCR Primers Composition comments This product does not contain any hazardous ingredients, or ingredients with national workplace exposure limits. SECTION 4: First aid measures 4.1. Description of first aid measures Inhalation Ingestion Skin contact Eye contact Protection of first aiders Not considered to be a significant hazard due to the small quantities used. Get medical attention if any discomfort continues. Show this Safety Data Sheet to the medical personnel. Move affected person to fresh air and keep warm and at rest in a position comfortable for breathing. Maintain an open airway. Loosen tight clothing such as collar, tie or belt. Rinse mouth thoroughly with water. Give a few small glasses of water or milk to drink. Stop if the affected person feels sick as vomiting may be dangerous. Do not induce vomiting unless under the direction of medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Never give anything by mouth to an unconscious person. Remove affected person from source of contamination. Rinse immediately with plenty of water. Rinse immediately with plenty of water. Remove any contact lenses and open eyelids wide apart. Continue to rinse for at least 10 minutes. First aid personnel should wear appropriate protective equipment during any rescue Most important symptoms and effects, both acute and delayed Inhalation Ingestion Skin contact Eye contact See Section 11 for additional information on health hazards. The severity of the symptoms described will vary dependent on the concentration and the length of exposure. Not considered to be a significant hazard due to the small quantities used. Prolonged inhalation of high concentrations may damage respiratory system. Gastrointestinal symptoms, including upset stomach. Fumes from the stomach contents may be inhaled, resulting in the same symptoms as inhalation. Prolonged contact may cause dryness of the skin. May cause temporary eye irritation Indication of any immediate medical attention and special treatment needed Notes for the doctor Specific treatments Treat symptomatically. No special treatment required. SECTION 5: Firefighting measures 5.1. Extinguishing media Suitable extinguishing media Unsuitable extinguishing media The product is not flammable. Extinguish with alcohol-resistant foam, carbon dioxide, dry powder or water fog. Use fire-extinguishing media suitable for the surrounding fire. Do not use water jet as an extinguisher, as this will spread the fire Special hazards arising from the substance or mixture Specific hazards Hazardous combustion products Containers can burst violently or explode when heated, due to excessive pressure build-up. Not considered to be a significant hazard due to the small quantities used. Thermal decomposition or combustion products may include the following substances: Harmful gases or vapours Advice for firefighters 2/8
73 PCR Primers Protective actions during firefighting Special protective equipment for firefighters Avoid breathing fire gases or vapours. Evacuate area. Cool containers exposed to heat with water spray and remove them from the fire area if it can be done without risk. Cool containers exposed to flames with water until well after the fire is out. If a leak or spill has not ignited, use water spray to disperse vapours and protect men stopping the leak. Wear positive-pressure self-contained breathing apparatus (SCBA) and appropriate protective clothing. Firefighter's clothing conforming to European standard EN469 (including helmets, protective boots and gloves) will provide a basic level of protection for chemical incidents. SECTION 6: Accidental release measures 6.1. Personal precautions, protective equipment and emergency procedures Personal precautions No action shall be taken without appropriate training or involving any personal risk. Keep unnecessary and unprotected personnel away from the spillage. Wear protective clothing as described in Section 8 of this safety data sheet. Wash thoroughly after dealing with a spillage Environmental precautions Environmental precautions Avoid discharge to the aquatic environment. Large Spillages: Inform the relevant authorities if environmental pollution occurs (sewers, waterways, soil or air) Methods and material for containment and cleaning up Methods for cleaning up Wear protective clothing as described in Section 8 of this safety data sheet. Reuse or recycle products wherever possible. If the product is soluble in water, dilute the spillage with water and mop it up. Flush contaminated area with plenty of water. Dispose of waste to licensed waste disposal site in accordance with the requirements of the local Waste Disposal Authority. Wash thoroughly after dealing with a spillage Reference to other sections Reference to other sections For personal protection, see Section 8. SECTION 7: Handling and storage 7.1. Precautions for safe handling Usage precautions Advice on general occupational hygiene Read and follow manufacturer's recommendations. Wear protective clothing as described in Section 8 of this safety data sheet. Keep away from food, drink and animal feeding stuffs. Handle all packages and containers carefully to minimise spills. Keep container tightly sealed when not in use. Avoid the formation of mists. Wash promptly if skin becomes contaminated. Take off contaminated clothing. Wash contaminated clothing before reuse. Do not eat, drink or smoke when using this product. Wash at the end of each work shift and before eating, smoking and using the toilet. Change work clothing daily before leaving workplace Conditions for safe storage, including any incompatibilities Storage precautions Storage class Store in accordance with local regulations. Unspecified storage Specific end use(s) Specific end use(s) The identified uses for this product are detailed in Section 1.2. SECTION 8: Exposure Controls/personal protection 8.1. Control parameters Ingredient comments No exposure limits known for ingredient(s) Exposure controls 3/8
74 PCR Primers Protective equipment Appropriate engineering controls Eye/face protection Hand protection Other skin and body protection Hygiene measures Respiratory protection Environmental exposure controls Provide adequate ventilation. Good general ventilation should be adequate to control worker exposure to airborne contaminants. Eyewear complying with an approved standard should be worn if a risk assessment indicates eye contact is possible. Personal protective equipment for eye and face protection should comply with European Standard EN166. The following protection should be worn: Chemical splash goggles. Chemical-resistant, impervious gloves complying with an approved standard should be worn if a risk assessment indicates skin contact is possible. The most suitable glove should be chosen in consultation with the glove supplier/manufacturer, who can provide information about the breakthrough time of the glove material. To protect hands from chemicals, gloves should comply with European Standard EN374. Considering the data specified by the glove manufacturer, check during use that the gloves are retaining their protective properties and change them as soon as any deterioration is detected. Frequent changes are recommended. Appropriate footwear and additional protective clothing complying with an approved standard should be worn if a risk assessment indicates skin contamination is possible. Provide eyewash station and safety shower. Contaminated work clothing should not be allowed out of the workplace. Wash contaminated clothing before reuse. Clean equipment and the work area every day. Good personal hygiene procedures should be implemented. Wash at the end of each work shift and before eating, smoking and using the toilet. When using do not eat, drink or smoke. Respiratory protection complying with an approved standard should be worn if a risk assessment indicates inhalation of contaminants is possible. Provide adequate ventilation. Large Spillages: If ventilation is inadequate, suitable respiratory protection must be worn. Not regarded as dangerous for the environment. SECTION 9: Physical and Chemical Properties 9.1. Information on basic physical and chemical properties Appearance Colour Odour Odour threshold ph Melting point Initial boiling point and range Flash point Evaporation rate Upper/lower flammability or explosive limits Vapour pressure Liquid. Colourless. Odourless. ~0 760 mm Hg ~ mm Hg 4/8
EXP-NBD103 Native Barcoding Kit 1D
EXP-NBD103 Native Barcoding Kit 1D Revision Date 27/11/2015 SAFETY DATA SHEET Native Barcode Adapters According to Regulation (EU) No 453/2010 SECTION 1: IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF
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