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1 Document No Respiration Test Chamber Copyright 2010 Terra Universal Inc. All rights reserved. Revised September 2010 Terra Universal, Inc. TerraUniversal.com 800 S. Raymond Ave. Fullerton, CA TEL: (714) FAX: (714)

2 Proprietary Notice This manual pertains to proprietary devices manufactured by Terra Universal, Inc. Neither this document nor any portion of it may be reproduced in any way without prior written permission from Terra Universal. Safety Notice A thorough familiarity with all operating guidelines is essential to safe operation of the product. Failure to observe safety precautions could result in poor performance, damage to the system or other property, or serious bodily injury or death. Terra Universal makes no warranties applying to information contained in this manual or its suitability for any implied or inferred purpose. Terra Universal shall not be held liable for any errors this manual contains or for any damages that result from its use. CAUTION Cautions are used when failure to observe instructions could result in significant damage to equipment. The following symbols are intended to call your attention to two levels of hazard involved in operation: The information presented here is subject to change without notice. WARNING Warnings are used when failure to observe instructions or precautions could result in injury or death. 1.0 Introduction This manual provides information on installing and operating your Terra Universal Respiration Test Chamber. By studying this document carefully, you can be assured of a long, efficient service life from the unit. 2.0 Description Terra Universal s Respiration Test Cabinet is designed to facilitate inhalation and respiration studies on rodents at precisely controlled oxygen concentrations. Fabricated from 304 stainless steel, it contains sixteen rodent enclosures: eight for mice (7.75"W x 13.5"D x 6.5"H each) and eight for rats (13.5"W x 16"D x 8.75"H each). These chambers accommodate standard mouse and rat cages. The top control panel includes four quadrant control sets. Each of these control sets feeds four mouse or four rat chambers (see photos below). Each quadrant control set contains these instruments: ON/OFF Switches: For oxygen and air Air/Oxygen Mixer: Provides mixture with precision of ± 3% of scale Mixed Gas Delivery Flow Meter: Directs selected gas mixture to the four target chambers with precision of ± 2% of scale Flow Meter Bypass: Bypasses the flow meter to allow rapid chamber conditioning at the desired oxygen concentration during equipment set up Terra Universal, Inc. TerraUniversal.com 800 S. Raymond Ave. Fullerton, CA TEL: (714) FAX: (714)

3 B C A D B A C D Letter Key A. Left Rat Quadrant B. Left Mouse Quadrant C. Right Mouse Quadrant D. Right Rat Quadrant The rear panel features connectors for 0.25" OD input oxygen and air lines, regulators for each gas line, and four sampling ports (one for each of the control sets). NOTE: Sampling ports allow connection of an Oxygen Analyzer, which can be used to validate the gas mixer for each quadrant control set. Each sample port features a female slip fitting, with a male fitting for connection to a sample tube. When the male fitting is disconnected, both fittings are closed to prevent contamination. Once the male fitting is engaged, the line can be pumped by the Oxygen Analyzer. In this state, the system is open to contaminants, so the male fitting should never be engaged unless the unit is properly connected to an Oxygen Analyzer equipped with a pump featuring a bypass valve. The sample ports draw gas samples from only one of the four chambers operated by each control set. For the two rat chamber quadrants, only the top chamber is sampled. For the two mouse chamber quadrants, only the bottom chamber is sampled. Terra Universal, Inc. TerraUniversal.com 800 S. Raymond Ave. Fullerton, CA TEL: (714) FAX: (714)

4 3.0 Installation The Respiration Test Cabinet includes two filter housings accessible via six screws on the rear panel. One is for a charcoal filter, which removes urine odors. The other is for a 99.99% efficient HEPA filter (measured at 0.3 micron particles), which removes particles from the air mixture exiting the system. 1. Input Oxygen and Air Connections: This operation requires a compressed oxygen bottle and compressed air bottle with appropriate shut-off valves and pressure regulators. Each gas bottle s pressure regulator must have a compression fitting at the output that accepts 0.25"-OD poly tubing. With the valves on the compressed oxygen and air bottles turned to their OFF position, connect an appropriate length of 0.25"-OD poly tubing between the oxygen regulator and the Swagelok oxygen fitting located at the lower-left rear of the cabinet. Connect a second length of tubing between the compressed air bottle regulator and the Swagelok air fitting located at the lower-right rear of the cabinet. 2. Turn ON both the oxygen and air bottles and set their regulators to 60 psi (these can safely be set up to 70 psi). Set both the oxygen and air regulators mounted on the rear of the cabinet to 50 psi (these can safely be set between 30 and 70 psi). NOTE: The two regulators mounted on the rear cabinet panel must be set within 20 psi of each other. Otherwise, the system issues an audible alarm to indicate that the oxygen or air source cylinder is running out. The alarm continues to sound until the condition is corrected. 3. Oxygen and Air ON/OFF Switches: The front control panel includes oxygen and air ON/OFF switches for each control quadrant. Turn ON the switches for any quadrants that will be populated by rodents. NOTE: To conserve gas, keep switches OFF in chamber quadrants that are not populated with rodents. In the ON settings, a quadrant will bleed between 2.5 and 4.5 liters/minute, depending on the flow setting. 4. Set Desired Gas Mixture: For each quadrant populated by rodents, set the desired oxygen concentration. The gas mixers use decimal equivalents to indicate oxygen percentage, e.g.,.6 = 60% oxygen. 5. Set Desired Flow Rate: All settings are in milliliters/minute (flow meter scale: one atmosphere of pressure). Recommended flow rate: 2 ml/minute/chamber/rodent = 8 ml/minute minimum per quadrant per rodent (mouse or rat). For each additional rodent, increase flow meter setting by 8mL/minute 6. Turn Flow Bypass ON: Flip the bypass switch in the ON (up) position for each quadrant to be populated by rodents. This bypass setting is included to allow rapid chamber conditioning the four chambers in the selected quadrant will receive the full system flow rate to reach the desired oxygen concentration as quickly as possible. Once this concentration is established, turn the bypass switch OFF (down position). Recommended conditioning times: Chambers with Rats: psi Chambers with Mice: 5 50 psi Terra Universal, Inc. TerraUniversal.com 800 S. Raymond Ave. Fullerton, CA TEL: (714) FAX: (714)

5 This chamber conditioning routine must be performed each time the oxygen percentage is changed or a door is opened. If the bypass setting is not used (i.e., gas flow is restricted by the flow meter), it may take up to 30 hours to condition chambers, even with the flow meter at the highest setting. 4.0 Maintenance Periodically, wipe down all surfaces with a clean, lint-free wiper (nonwoven polyester is recommended for optimal cleanliness). Use alcohol or appropriate disinfectant. Charcoal and final HEPA filters should be replaced once each year (more often if animal odors become noticeable). To replace filters, simply remove the six screws that hold the filter cover on the rear of the unit. Remove and replace with new filters, being carefully not to damage the filter face. Replacement Charcoal Filter (12" x 12" x 2") Cat. # A Replacement HEPA Filter (12" x 12" x 3") Cat. # Specifications Construction Dimensions Gas Mixers Accuracy Flow Meter Accuracy Line Fittings Filters 304 stainless steel with PVC viewing windows 30.25"W x 24"D x 77"H (excluding leveling feet) ± 3% of scale ± 2% of scale Quick-connect Swagelok fittings for.25" OD polyethylene tubing 12" x 12" activated charcoal (for odor removal); 12" x 12" final HEPA (rated 99.99% 0.3 micron particles) Terra Universal, Inc. TerraUniversal.com 800 S. Raymond Ave. Fullerton, CA TEL: (714) FAX: (714)

6 6.0 Warranty Products Manufactured by Terra: Terra Universal, Inc., warrants products that it manufactures to be free from defects for a period of 12 months for parts and 90 days for labor, commencing from the date of shipment. Terra s sole responsibility is to repair or replace, at its option, any part of the product that proves defective or malfunctioning during this time limit. In some cases, components incorporated in Terra Universal products are covered by additional warranties from component manufacturers; obtain specific information from Terra sales representatives. This warranty is void if the equipment is abused or modified by the customer, is operated outside Terra s operating instructions or specifications, or is used in any application other than that for which it is specified. This warranty does not include routine maintenance or service procedures, breakage of quartz baths after 60 days, shipping damage, nor damage from misuse, intentional or unintentional abuse, neglect, natural disasters, or acts of God. Products Manufactured by Others: Terra Universal, Inc., warrants that, to the best of its ability, Terra s representations of products that are manufactured by others reflect the manufacturer s representations, subject to change without notice. Sole warranty for these products is the original manufacturer s warranty that is passed forward to the purchaser and constitutes the customer s sole remedy for these products. Detailed warranties for distributed products are available through Terra sales representatives. Freight Shortage or Damage: Upon receipt of any equipment from Terra Universal, Inc., customer shall immediately unpack and inspect for damage or shortage. The customer shall not accept a damaged package or a short shipment until the carrier makes a "damage or shortage" notation on both the carrier's and customer's copy of the freight bill or delivery receipt. Service title passes when the shipment is loaded, so customer is responsible for filing and collecting a freight claim. Any replacement products must be ordered and paid for separately. For Terra's "Policy and Procedures for Returning Goods," see Terra's Internet site: Generally, customers can improve the chance of collecting on a freight claim by following these procedures: 1) formally requesting that the carrier inspect the shipment immediately upon suspecting damage or shortage to verify condition; 2) notifying the carrier upon discovery of concealed damage and requesting an inspection within 15 days of receipt, both in person or phone and following up via mail; 3) keeping the shipment as intact as possible, including retaining original packaging materials and keeping the product as close to the original receiving location as possible; 4) holding salvage for disposition by the carrier. All Claims: Terra Universal expressly disclaims all other warranties, expressed or implied or implied by statute, including the warranties of merchantability or fitness for intended use. Terra Universal is not responsible for consequential or incidental damages arising out of the purchase or use of the products supplied by Terra Universal. Terra Universal is not liable for damage to facilities, other equipment, products, property or personnel of others, or of their agents, suppliers, or affiliated parties, which is caused or alleged to have been caused by products supplied by Terra Universal. In any event or series of events, Terra Universal s total liability for any and all damages whatsoever is limited to the lesser of the actual damages or the original invoice cost of the items alleged to have caused the damage. The customer s sole and exclusive remedy for any cause of action whatsoever is repair or replacement of the non-conforming products or refund of the actual purchase price, at the sole option of Terra Universal. All claims must be made in writing within 90 days of the date the product was shipped. Any claims not made within this time limit shall be deemed waived by the customer. Terra Universal is not responsible for any additional costs of repair caused by poor packaging or in-shipment damage during return. Warranty Returns: All warranty returns must be authorized in advance by Terra Universal and approved under an RMA. Unless approved in advance for good reason, all returns must be in original condition, including all manuals, and must be packaged in original packaging materials. All returned goods are to be shipped to Terra Universal, freight prepaid at customer s expense. See Terra s Policy and Procedure for Returned Goods. Thank you for ordering from Terra Universal! Terra Universal, Inc. TerraUniversal.com 800 S. Raymond Ave. Fullerton, CA TEL: (714) FAX: (714)

92831 TEL: (714) FAX:

92831 TEL: (714) FAX: Document N0. 1800-03 Copyright 2010 Terra Universal Inc. All rights reserved. Revised Sept. 2010 Terra Universal, Inc. TerraUniversal.com 800 S. Raymond Ave. Fullerton, CA 92831 TEL: (714) 578-6000 FAX:

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