Brilliant QPCR Master Mix, Catalog # Reference dye, 1mM Identified uses. Mixture
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1 SAFETY DATA SHEET Brilliant QPCR Master, Catalog # SECTION 1 Identification of the substance/mixture and of the company/undertaking 1.1 Product identifier Product name Brilliant QPCR Master, Catalog # Part No. (Kit) Part No Relevant identified uses of the substance or mixture and uses advised against Analytical reagent. 5 ml (2.5 ml / Tube) 0.1 ml Identified uses 1.3 Details of the supplier of the safety data sheet Agilent Technologies Manufacturing GmbH & Co. KG Hewlett-Packard-Str Waldbronn Germany address of person responsible for this SDS pdl-msds_author@agilent.com 1.4 Emergency telephone number Emergency telephone number (with hours of operation) Chemtrec +(44) SECTION 2 Hazards identification 2.1 Classification of the substance or mixture Product definition Classification according to Regulation (EC) No. 1272/2008 [CLP/GHS] Master H373i SPECIFIC TARGET ORGAN TOXICITY (REPEATED EXPOSURE) INHALATION [kidneys] - Category 2 Classification Classification according to Directive 1999/45/EC [DPD] ture ture The product is not classified as dangerous according to Directive 1999/45/EC and its amendments. The product is not classified as dangerous according to Directive 1999/45/EC and its amendments. Not classified. Not classified. See Section 16 for the full text of the R phrases or H statements declared above. See Section 11 for more detailed information on health effects and symptoms. 29/05/2011 1/13
2 Brilliant QPCR Master, Catalog # SECTION 2 Hazards identification 2.2 Label elements Hazard pictograms Signal word Hazard statements Precautionary statements Warning No signal word. GHS08 - May cause damage to organs through prolonged or repeated exposure if inhaled. (kidneys) Prevention Do not breathe vapour. Response Get medical attention if you feel unwell. Storage Disposal Hazardous ingredients Glycerol Supplemental label elements Special packaging requirements Tactile warning of danger 2.3 Other hazards Other hazards which do not result in classification SECTION 3 Composition/information on ingredients Substance/mixture Master Product/ingredient name Identifiers ture ture Classification % 67/548/EEC Regulation (EC) No. Type 1272/2008 [CLP] Master Glycerol EC CAS Not classified. STOT RE 2, H373i [1] [2] See section 16 for the full text of the R- phrases declared above See Section 16 for the full text of the H statements declared above. 29/05/2011 2/13
3 Brilliant QPCR Master, Catalog # SECTION 3 Composition/information on ingredients There are no additional ingredients present which, within the current knowledge of the supplier and in the concentrations applicable, are classified as hazardous to health or the environment and hence require reporting in this section. Type [1] Substance classified with a health or environmental hazard [2] Substance with a workplace exposure limit [3] Substance meets the criteria for PBT according to Regulation (EC) No. 1907/2006, Annex XIII [4] Substance meets the criteria for vpvb according to Regulation (EC) No. 1907/2006, Annex XIII Occupational exposure limits, if available, are listed in Section 8. SECTION 4 First aid measures 4.1 Description of first aid measures Eye contact Inhalation Skin contact Ingestion Immediately flush eyes with plenty of water, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Continue to rinse for at least 10 minutes. Get medical attention following exposure or if feeling unwell. Immediately flush eyes with plenty of water, occasionally lifting the upper and lower eyelids. Check for and remove any contact lenses. Get medical attention if irritation occurs. Remove victim to fresh air and keep at rest in a position comfortable for breathing. If not breathing, if breathing is irregular or if respiratory arrest occurs, provide artificial respiration or oxygen by trained personnel. It may be dangerous to the person providing aid to give mouth-tomouth resuscitation. Get medical attention following exposure or if feeling unwell. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Remove victim to fresh air and keep at rest in a position comfortable for breathing. Get medical attention if symptoms occur. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Continue to rinse for at least 10 minutes. Get medical attention following exposure or if feeling unwell. Wash clothing before reuse. Clean shoes thoroughly before reuse. Flush contaminated skin with plenty of water. Remove contaminated clothing and shoes. Get medical attention if symptoms occur. Wash out mouth with water. Remove dentures if any. Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Stop if the exposed person feels sick as vomiting may be dangerous. Do not induce vomiting unless directed to do so by medical personnel. If vomiting occurs, the head should be kept low so that vomit does not enter the lungs. Get medical attention following exposure or if feeling unwell. Never give anything by mouth to an unconscious person. If unconscious, place in recovery position and get medical attention immediately. Maintain an open airway. Loosen tight clothing such as a collar, tie, belt or waistband. Wash out mouth with water. Remove victim to fresh air and keep at rest in a position comfortable for breathing. If material has been swallowed and the exposed person is conscious, give small quantities of water to drink. Do not induce vomiting unless directed to do so by medical personnel. Get medical attention if symptoms occur. 29/05/2011 3/13
4 Brilliant QPCR Master, Catalog # SECTION 4 First aid measures Protection of first-aiders No action shall be taken involving any personal risk or without suitable training. It may be dangerous to the person providing aid to give mouth-to-mouth resuscitation. No action shall be taken involving any personal risk or without suitable training. 4.2 Most important symptoms and effects, both acute and delayed Potential acute health effects Eye contact Inhalation Skin contact Ingestion Over-exposure signs/symptoms Eye contact Inhalation Skin contact Ingestion 4.3 Indication of any immediate medical attention and special treatment needed Notes to physician Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled. Treat symptomatically. Contact poison treatment specialist immediately if large quantities have been ingested or inhaled. Specific treatments SECTION 5 Firefighting measures 5.1 Extinguishing media Suitable extinguishing media Unsuitable extinguishing media No specific treatment. No specific treatment. Use an extinguishing agent suitable for the surrounding fire. Use an extinguishing agent suitable for the surrounding fire. None known. None known. 5.2 Special hazards arising from the substance or mixture Hazards from the substance or mixture In a fire or if heated, a pressure increase will occur and the container may burst. In a fire or if heated, a pressure increase will occur and the container may burst. 29/05/2011 4/13
5 Brilliant QPCR Master, Catalog # SECTION 5 Firefighting measures Hazardous combustion products Decomposition products may include the following materials carbon dioxide carbon monoxide 5.3 Advice for firefighters Special precautions for fire-fighters Special protective equipment for firefighters Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. Promptly isolate the scene by removing all persons from the vicinity of the incident if there is a fire. No action shall be taken involving any personal risk or without suitable training. Fire-fighters should wear appropriate protective equipment and self-contained breathing apparatus (SCBA) with a full face-piece operated in positive pressure mode. Clothing for fire-fighters (including helmets, protective boots and gloves) conforming to European standard EN 469 will provide a basic level of protection for chemical incidents. SECTION 6 Accidental release measures 6.1 Personal precautions, protective equipment and emergency procedures For non-emergency personnel For emergency responders No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilt material. Avoid breathing vapour or mist. Provide adequate ventilation. Wear appropriate respirator when ventilation is inadequate. Put on appropriate personal protective equipment. No action shall be taken involving any personal risk or without suitable training. Evacuate surrounding areas. Keep unnecessary and unprotected personnel from entering. Do not touch or walk through spilt material. Put on appropriate personal protective equipment. If specialised clothing is required to deal with the spillage, take note of any information in Section 8 on suitable and unsuitable materials. See also the information in "For nonemergency personnel". If specialised clothing is required to deal with the spillage, take note of any information in Section 8 on suitable and unsuitable materials. See also the information in "For nonemergency personnel". 6.2 Environmental precautions Avoid dispersal of spilt material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). Avoid dispersal of spilt material and runoff and contact with soil, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution (sewers, waterways, soil or air). 6.3 Methods and materials for containment and cleaning up Methods for cleaning up Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of via a licensed waste disposal contractor. Stop leak if without risk. Move containers from spill area. Dilute with water and mop up if water-soluble. Alternatively, or if water-insoluble, absorb with an inert dry material and place in an appropriate waste disposal container. Dispose of 29/05/2011 5/13
6 Brilliant QPCR Master, Catalog # SECTION 6 Accidental release measures 6.4 Reference to other sections SECTION 7 Handling and storage 7.1 Precautions for safe handling Protective measures Advice on general occupational hygiene via a licensed waste disposal contractor. See Section 1 for emergency contact information. See Section 8 for information on appropriate personal protective equipment. See Section 13 for additional waste treatment information. Put on appropriate personal protective equipment (see Section 8). Do not breathe vapour or mist. Do not ingest. Avoid contact with eyes, skin and clothing. Keep in the original container or an approved alternative made from a compatible material, kept tightly closed when not in use. Empty containers retain product residue and can be hazardous. Do not reuse container. Put on appropriate personal protective equipment (see Section 8). Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures. Eating, drinking and smoking should be prohibited in areas where this material is handled, stored and processed. Workers should wash hands and face before eating, drinking and smoking. Remove contaminated clothing and protective equipment before entering eating areas. See also Section 8 for additional information on hygiene measures. 7.2 Conditions for safe storage, including any incompatibilities Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabelled containers. Use appropriate containment to avoid environmental contamination. Store in accordance with local regulations. Store in original container protected from direct sunlight in a dry, cool and well-ventilated area, away from incompatible materials (see section 10) and food and drink. Keep container tightly closed and sealed until ready for use. Containers that have been opened must be carefully resealed and kept upright to prevent leakage. Do not store in unlabelled containers. Use appropriate containment to avoid environmental contamination. 7.3 Specific end use(s) Recommendations Industrial sector specific solutions Industrial applications, Professional applications. Industrial applications, Professional applications. 29/05/2011 6/13
7 Brilliant QPCR Master, Catalog # SECTION 8 Exposure controls/personal protection 8.1 Control parameters Occupational exposure limits Product/ingredient name Exposure limit values Glycerol ACGIH TLV (United States, 2/2010). TWA 10 mg/m³ 8 hour(s). Form Inhalable fraction Recommended monitoring procedures Derived effect levels No DNELs available. If this product contains ingredients with exposure limits, personal, workplace atmosphere or biological monitoring may be required to determine the effectiveness of the ventilation or other control measures and/or the necessity to use respiratory protective equipment. Reference should be made to European Standard EN 689 for methods for the assessment of exposure by inhalation to chemical agents and national guidance documents for methods for the determination of hazardous substances. Predicted effect concentrations No PNECs available. 8.2 Exposure controls Appropriate engineering controls Individual protection measures Hygiene measures Eye/face protection Skin protection Hand protection Body protection Other skin protection Respiratory protection Environmental exposure controls If user operations generate dust, fumes, gas, vapour or mist, use process enclosures, local exhaust ventilation or other engineering controls to keep worker exposure to airborne contaminants below any recommended or statutory limits. Wash hands, forearms and face thoroughly after handling chemical products, before eating, smoking and using the lavatory and at the end of the working period. Appropriate techniques should be used to remove potentially contaminated clothing. Wash contaminated clothing before reusing. Ensure that eyewash stations and safety showers are close to the workstation location. Safety eyewear complying with an approved standard should be used when a risk assessment indicates this is necessary to avoid exposure to liquid splashes, mists, gases or dusts. Chemical-resistant, impervious gloves complying with an approved standard should be worn at all times when handling chemical products if a risk assessment indicates this is necessary. Personal protective equipment for the body should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Appropriate footwear and any additional skin protection measures should be selected based on the task being performed and the risks involved and should be approved by a specialist before handling this product. Use a properly fitted, air-purifying or air-fed respirator complying with an approved standard if a risk assessment indicates this is necessary. Respirator selection must be based on known or anticipated exposure levels, the hazards of the product and the safe working limits of the selected respirator. Emissions from ventilation or work process equipment should be checked to ensure they comply with the requirements of environmental protection legislation. In some cases, fume scrubbers, filters or engineering modifications to the process equipment will be necessary to reduce emissions to acceptable levels. 29/05/2011 7/13
8 Brilliant QPCR Master, Catalog # SECTION 9 Physical and chemical properties 9.1 Information on basic physical and chemical properties Appearance Physical state Colour Odour Odour threshold ph Melting point/freezing point Initial boiling point and boiling range Flash point Evaporation rate Flammability (solid, gas) Upper/lower flammability or explosive limits Vapour pressure Vapour density Relative density Solubility(ies) Partition coefficient n- octanol/water Auto-ignition temperature Decomposition temperature Master Master Master Master Liquid. Liquid. 8 Master 8 Master 0 C Master 100 C Master Master Master Master Master Master Master Master Master Master Master [Product does not sustain combustion.] Easily soluble in the following materials cold water and hot water. Easily soluble in the following materials cold water and hot water. 29/05/2011 8/13
9 Brilliant QPCR Master, Catalog # SECTION 9 Physical and chemical properties Viscosity Explosive properties Master Master 9.2 Other information No additional information. SECTION 10 Stability and reactivity 10.1 Reactivity No specific test data related to reactivity available for this product or its ingredients. No specific test data related to reactivity available for this product or its ingredients Chemical stability The product is stable. The product is stable Possibility of hazardous reactions Under normal conditions of storage and use, hazardous reactions will not occur. Under normal conditions of storage and use, hazardous reactions will not occur Conditions to avoid 10.5 Incompatible materials 10.6 Hazardous decomposition products SECTION 11 Toxicological information 11.1 Information on toxicological effects Acute toxicity Under normal conditions of storage and use, hazardous decomposition products should not be produced. Under normal conditions of storage and use, hazardous decomposition products should not be produced. Product/ingredient name Result Species Dose Exposure Glycerol LD50 Oral Rat mg/kg - Acute toxicity estimates Irritation/Corrosion Product/ingredient name Result Species Score Exposure Observation Glycerol Eyes - Mild irritant Rabbit - 24 hours 500 milligrams Skin - Mild irritant Rabbit - 24 hours 500 milligrams /05/2011 9/13
10 Brilliant QPCR Master, Catalog # SECTION 11 Toxicological information Sensitiser Conclusion/Summary Potential acute health effects Inhalation Ingestion Skin contact Eye contact Symptoms related to the physical, chemical and toxicological characteristics Inhalation Ingestion Skin contact Eye contact Chronic toxicity / Carcinogenicity / Mutagenicity / Teratogenicity / Reproductive toxicity Specific target organ toxicity (single exposure) Specific target organ toxicity (repeated exposure) Aspiration hazard Information on the likely routes of exposure Product/ingredient name Category Route of exposure Glycerol Category 2 Inhalation kidneys Delayed and immediate effects and also chronic effects from short and long term exposure Short term exposure Potential immediate effects Potential delayed effects Long term exposure Potential immediate effects Potential delayed effects Target organs 29/05/ /13
11 Brilliant QPCR Master, Catalog # SECTION 11 Toxicological information Potential chronic health effects General Carcinogenicity Mutagenicity Teratogenicity Developmental effects Fertility effects Toxicokinetics Absorption Distribution Metabolism Elimination Other information SECTION 12 Ecological information 12.1 Toxicity Product/ingredient name Result May cause damage to organs through prolonged or repeated exposure if inhaled. Species Glycerol Acute LC50 54 to 57 ml/l Fresh water Fish - Oncorhynchus mykiss g Exposure 96 hours 12.2 Persistence and degradability Conclusion/Summary 12.3 Bioaccumulative potential Product/ingredient name LogPow BCF Potential Glycerol low 12.4 Mobility in soil Soil/water partition coefficient (KOC) Mobility 29/05/ /13
12 Brilliant QPCR Master, Catalog # SECTION 12 Ecological information 12.5 Results of PBT and vpvb assessment PBT vpvb 12.6 Other adverse effects SECTION 13 Disposal considerations 13.1 Waste treatment methods Product Methods of disposal Hazardous waste Packaging Methods of disposal Special precautions SECTION 14 Transport information The generation of waste should be avoided or minimised wherever possible. Significant quantities of waste product residues should not be disposed of via the foul sewer but processed in a suitable effluent treatment plant. Dispose of surplus and non-recyclable products via a licensed waste disposal contractor. Disposal of this product, solutions and any by-products should at all times comply with the requirements of environmental protection and waste disposal legislation and any regional local authority requirements. The classification of the product may meet the criteria for a hazardous waste. The generation of waste should be avoided or minimised wherever possible. Waste packaging should be recycled. Incineration or landfill should only be considered when recycling is not feasible. This material and its container must be disposed of in a safe way. Care should be taken when handling emptied containers that have not been cleaned or rinsed out. Empty containers or liners may retain some product residues. Avoid dispersal of spilt material and runoff and contact with soil, waterways, drains and sewers. Regulatory information ADR/RID / IMDG / IATA Not regulated Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code SECTION 15 Regulatory information 15.1 Safety, health and environmental regulations/legislation specific for the substance or mixture EU Regulation (EC) No. 1907/2006 (REACH) Annex XIV - List of substances subject to authorisation Substances of very high concern None of the components are listed. Annex XVII - Restrictions on the manufacture, placing on the market and use of certain dangerous substances, mixtures and articles Other EU regulations Europe inventory Black List Chemicals Priority List Chemicals At least one component is not listed. Not listed Not listed 29/05/ /13
13 Brilliant QPCR Master, Catalog # SECTION 15 Regulatory information Integrated pollution prevention and control list (IPPC) - Air Integrated pollution prevention and control list (IPPC) - Water Not listed Not listed 15.2 Chemical Safety Assessment SECTION 16 Other information Full text of abbreviated R phrases This product contains substances for which Chemical Safety Assessments might still be required. Indicates information that has changed from previously issued version. Abbreviations and acronyms Full text of abbreviated H statements Full text of classifications [DSD/DPD] ATE = Acute Toxicity Estimate CLP = Classification, Labelling and Packaging Regulation [Regulation (EC) No. 1272/2008] DNEL = Derived No Effect Level EUH statement = CLP-specific Hazard statement PNEC = Predicted No Effect Concentration RRN = REACH Registration Number Procedure used to derive the classification according to Regulation (EC) No. 1272/2008 [CLP/GHS] Classification STOT RE 2, H373i Full text of classifications [CLP/GHS] Date of issue/ Date of Date of previous issue Version Notice to reader Master H373i Master STOT RE 2, H373i 29/05/2011 No previous validation. 2 Calculation method SPECIFIC TARGET ORGAN TOXICITY (REPEATED EXPOSURE) INHALATION [kidneys] - Category 2 Justification May cause damage to organs through prolonged or repeated exposure if inhaled. Disclaimer The information contained in this document is based on Agilent s state of knowledge at the time of preparation. No warranty as to its accurateness, completeness or suitability for a particular purpose is expressed or implied. 29/05/ /13
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