PRODUCT LIST 2014 MICROBIOLOGY

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1 PRODUCT LIST 2014 MICROBIOLOGY

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3 Contents Introduction 4 Alternative methods validated by AFNOR Certification 5-6 Suspensions, dilutions, revivifications 7 Anaerobic bacteria 7 Anaerobic sulfito-reducing bacteria 7 Bacillus 8 Clostridium 8 Cronobacter spp. 8 Enterobacteriaceae 8 Compass Bacillus Agar 9-10 Compass Enterobactar sakazakii Escherichia coli, coliforms 13 Escherichia coli O Total Viable Count 14 Lactobacillus, Leuconostoc, Lactococcus 15 Legionella 15 Listeria 16 Compass Listeria Agar Rhapsody Agar Pseudomonas 21 Salmonella, Shigella Compass Salmonella Agar Staphylococcus 25 Streptococcus, Enterococcus 25 Vibrio 26 Yeasts and molds 26 Antibiotic susceptibility testing 27 Blood agar base 27 Culture media base 27 Compass Enterococcus Agar Accessory 30 Water range Cosmetic range

4 Introduction Quality and products reliability BIOKAR Diagnostics performs strict controls to each manufacturing step, targeting the raw materials, in-process intermediate products and the final end product. All production batches are released for sale only when they meet or exceed pre-defined acceptation criteria (microbiological performance, stability, ph,...). In case of need, a sample is kept for reference in a sample collection for each batch for duration of its validity. Answers to your microbiological needs The certification ISO 9001:2008 for the production of culture media and associated supplements is only one tangible demonstration of the objective BIOKAR Diagnostics has fixed in terms of the commercialization of consistent and optimal quality product. BIOKAR Diagnostics offers media conforms to international standards and innovative products validated by AFNOR Certification. 4

5 Alternative methods validated by AFNOR Certification IRIS Salmonella RIS Salmonella method allows a rapid detection of all Salmonellae in human, animal food products and environmental samples. Enrichment media Salmonella Enrichment Salmonella Enrichment Salmonella Enrichment with Tween 80 - ready-to-use medium Salmonella Enrichment - ready-to-use medium Salmonella Enrichment - ready-to-use medium Salmonella Enrichment - ready-to-use medium Isolation and identification media IRIS Salmonella Agar - pre-poured medium IRIS Salmonella Agar - pre-poured medium IRIS Salmonella supplement IRIS Salmonella liquid supplement Confirmation test CONFIRM Salmonella - Latex agglutination kit BK194GC 5 kg BK194HA 500 g BM x3L in flexible bags BM x225mL in vials BM x3L in flexible bags BM x5L in flexible bags BM plates Ø 90mm BM plates Ø 90mm BS tablets BS x50m L in vials BT tests COMPASS Listeria COMPASS Listeria method allows the detection and enumeration of Listeria monocytogenes and Listeria spp. in the food and environmental samples products. Diluant Buffered peptone water (20 g/l) BK131HA 500 g Buffered peptone water (20 g/l) BK131GC 5 kg Buffered peptone water (25,5 g/l) BK018HA 500 g Buffered peptone water (25,5 g/l) BK018GC 5 kg Buffered peptone water (25,5 g/l) - ready-to-use medium BM x225m L in vials Buffered peptone water (25,5 g/l) - ready-to-use medium BM x9m L in tubes Buffered peptone water (25,5 g/l) - ready-to-use medium BM x90m L in vials Buffered peptone water (25,5 g/l) - ready-to-use medium BM x3L in flexible bags Buffered peptone water (25,5 g/l) - ready-to-use medium BM x5L in flexible bags Enrichment media Half-FRASER Broth - dehydrated base medium BK173HA 500 g Half-FRASER Broth - dehydrated base medium BK173HA 5 kg Half-FRASER Broth selective supplement BS vials pack qsp 500 ml Half-FRASER Broth selective supplement BS vials pack qsp 2,25 L Half-FRASER Broth - ready-to-use BM x225m L in vials Half-FRASER Broth - ready-to-use BM x5L in flexible bags Half-FRASER Broth - ready-to-use BM x3L in flexible bags Isolation and identification media COMPASS Listeria Agar (Kit) BT x 200 ml base medium in vials + 6 vials of selective suppl. qsp 200 ml + 6 vials of enrichment suppl. qsp 200 ml COMPASS Listeria Agar - pre-poured medium BM plates Ø 90 mm COMPASS Listeria Agar - pre-poured medium BM plates Ø 90 mm Confirmation test CONFIRM L.mono Broth BM vials with 1 ml CONFIRM L.mono Agar - pre-poured medium BM plates Ø 90 mm Validated according to ISO by Afnor Certification under reference BKR 23/07-10/11. More informations on afnorcerfication.org Validated according to ISO by Afnor Certification under reference BKR 23/02-11/02 (detection) et BKR 23/05-12/07 (enumeration). More informations on afnorcertification.org 5

6 Alternative methods validated by AFNOR Certification COMPASS Bacillus COMPASS Bacillus method allows the detection and enumeration of spores and vegetative forms of the species belonging to the group of presumptive Bacillus cereus in the food and feed products. Isolation and identification media COMPASS Bacillus cereus Agar - pre-poured medium COMPASS Bacillus cereus Agar - ready-to-melt medium COMPASS Bacillus cereus Agar selective supplement COMPASS cc BM plates Ø 90 mm BM x 100 ml in vials BS vials pack qsp 100 ml SESAME Salmonella TEST SESAME Salmonella TEST method allows the detection of motile Salmonella in the food, feed and environmental samples products. Enrichment media Salmonella Enrichment Salmonella Enrichment Salmonella Enrichment with Tween 80 - ready-to-use medium Salmonella Enrichment - ready-to-use medium Salmonella Enrichment - ready-to-use medium Salmonella Enrichment - ready-to-use medium SESAME Salmonella TEST method SESAME Salmonella Detection SESAME Salmonella Detection - pre-poured medium SESAME Salmonella Detection - pre-poured medium SESAME Salmonella Detection - ready-to-melt medium Confirmation test COMPASS Salmonella Agar - pre-poured medium COMPASS cc method allows the enumeration of Escherichia coli and coliforms bacteria in water human consumption. Isolation and identification media COMPASS cc Agar - pre-poured medium COMPASS cc Agar - dehydrated base medium COMPASS cc Agar selective supplement BK194GC 5 kg BK194HA 500 g BM x3L in flexible bags BM x225m L in vials BM x3L in flexible bags BM x5L in flexible bags BK195HA 500 g BM plates Ø 90 mm BM plates Ø 90 mm BM x200mLinvial BM plates Ø 90 mm BM plates Ø 55 mm BK210HA 500 g BS vials pack qsp 500 ml Validated according to ISO by Afnor Certification under reference BKR 23/06-02/10. More informations on afnorcerfication.org Validated according to ISO by Afnor Certification under reference BKR 23/04-12/07. More informations on afnorcerfication.org Validated according to the reference method protocol by Afnor Certification under reference BKR 23/08-06/12. More informations on afnorcertification.org 6

7 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Suspensions, dilutions, revivifications Tryptone-salt Broth (Maximum Recovery Diluent) BK014HA 500 g Tryptone-salt Broth (Maximum Recovery Diluent) - Ready-to-use medium BM x9mL in tubes Tryptone-salt Broth (Maximum Recovery Diluent) - Ready-to-use medium BM x90mL in vials Tryptone-salt Broth (Maximum Recovery Diluent) - Ready-to-use medium BM x3L in flexible bags Buffered peptone Broth with sodium chloride ph 7.0 BK128HA 500 g Lactose Broth BK082HA 500 g Sterile distilled water BM x18mL in tubes Peptone water 0,1 % BM x5L in flexible bags Buffered peptone water (25.5 g/l) BK018GC 5 kg Buffered peptone water (25.5 g/l) BK018HA 500 g Buffered peptone water (20 g/l) BK131GC 5 kg Buffered peptone water (20 g/l) BK131HA 500 g Buffered peptone water (25.5 g/l) BM x225mL in vials Buffered peptone water (25.5 g/l) BM x9mL in tubes Buffered peptone water (25.5 g/l) BM x90mL in vials Buffered peptone water (25.5 g/l) BM x3L in flexible bags Buffered peptone water (25.5 g/l) BM x5L in flexible bags Peptone water BK084HA 500 g Salmonella Enrichment with Tween 80 - Ready-to-use BM x3L in flexible bags Ringer's solution (1/4 strength) BR tablets Anaerobic bacteria Meat liver glucose 0.6 % Agar BK024HA 500 g Reinforced Clostridial Medium (RCM) Broth BK094HA 500 g Reinforced Clostridial Agar BK090HA 500 g Anaerobic sulfito-reducing bacteria TSN Agar BK001HA 500 g Meat-yeast Agar BK006HA 500 g Meat-liver glucose Agar BK157HA 500 g Meat liver glucose agar with 2g/L yeast extract BM x200mL in vials TSC Agar - Ready-to-use base medium BM x20mL in tubes TSC Agar - Ready-to-melt base medium BM x200mL in vials TSC Agar - Dehydrated base medium BK031HA 500 g Incubation Characteristics 24 hours at 37 C (anaerobic conditions) Back colonies, often surrounded by a back precipitate (reduction of sodium sulfite) * D-cycloserine 200mg selective supplement BS vials pack qsp 500mL * Egg Yolk Enrichment BS x50mL in vials 7

8 CLASSIFICATION - BY MICROORGANISMS 8 Designation Code Packaging Bacillus Dextrose Tryptone Agar BK042HA 500 g BCP Dextrose Agar - Ready-to-melt medium BM x200mL in vials Bacillus cereus (acc. to Mossel) - Pre-poured medium BM plates Ø 90 mm Bacillus cereus Agar (acc. to Mossel) - Dehydrated base medium BK116HA 500 g Egg Yolk Emulsion with Polymyxin B BS x50mL in vials Polymyxin B selective supplement BS vials pack Egg Yolk Enrichment BS x50mL in vials COMPASS Bacillus cereus Agar - Pre-poured medium BM plates Ø 90 mm COMPASS Bacillus cereus Agar (base) BK189HA 500 g COMPASS Bacillus cereus Agar - Ready-to-melt base medium BM x100mL in vials COMPASS Bacillus cereus - Selective supplement BS vials pack qsp 100 ml COMPASS Bacillus cereus - Selective supplement BS vials pack qsp 100 ml Clostridium Lactose sulfite (LS) Broth BK140HA 500 g BRYANT and BURKEY Broth with resazurin (modified acc. to BERGERE) BK141GC 5 kg BRYANT and BURKEY Broth with resazurin (modified acc. to BERGERE) BK141HA 500 g Thioglycollate medium with resazurin BK017HA 500 g Thioglycollate medium with resazurin - Ready-to-use medium BM x10mL in tubes TSC Agar - Ready-to-use base medium BM x10mL in tubes TSC Agar - Ready-to-melt base medium BM x200mL in vials TSC Agar - Dehydrated base medium BK031HA 500 g D-cycloserine 200 mg selective supplement BS vials pack qsp 500 ml Egg Yolk Enrichment BS x50mL in vials Cronobacter spp. Modified Lauryl Sulfate Tryptose (mlst) vancomycin Broth BK190HA 500 g Modified Lauryl Sulfate Tryptose (mlst) vancomycin Broth - Ready-to-use medium BM x10mL in tubes COMPASS Enterobacter sakazakii Agar - Pre-poured medium BM plates Ø 90 mm COMPASS Enterobacter sakazakii Agar BK188HA 500 g Enterobacteriaceae EE Broth (acc. to MOSSEL) BK140HA 500 g VRBG Agar - Ready-to-melt medium BK141GC 5 kg VRBG Agar BK141HA 500 g VRBG Agar BK017HA 500 g MUG 50 mg supplement BM x10mL in tubes Glucose Agar - Ready-to-use medium BM x10mL in tubes Bromcresol Purple Lactose Agar BM x200mL in vials KLIGLER Agar BK031HA 500 g DRIGALSKI lactose Agar BS vials pack qsp 500 ml TSI Agar BS x50mL in vials

9 Bacillus cereus Agar Dénombrement des Bacillus cereus sans confirmation Enumeration of Bacillus cereus without confirmation Sans confirmation Without confirmation Résultat en 24h Result in 24h thuringiensis pseudomycoïdes mycoïdes cereus weihenstephanensi Enzyme spécifique Specifical enzyme Pour les petits nombres For low numbers 9

10 ISO 7932 Comptage des colonies à 30 C Enumeration of colonies at 30 C Microbiologie des Aliments Food Microbiology Bacillus cereus Agar Dénombrement des Bacillus cereus présumés Enumeration of presumed Bacillus cereus ISO Technique NPP - petits nombres MPN protocol - low numbers Microbiologie des Aliments Food Microbiology Bacillus cereus Agar Ensemencement en surface Surface plating Microbiologie des Aliments et Alimentation Animale Food Microbiology & Animal Food Bacillus cereus Agar Ensemencementen profondeur pourlespetitsnombres Pour plate inoculation for low numbers Microbiologie des Aliments et Alimentation Animale Food Microbiology & Animal Food Prise d essai, ensemencement et dénombrement / Sample preparation, inoculation and enumeration EPT / BPW EPT / BPW EPT / BPW 1 MOSSEL / MYP TSPB Bacillus cereus Agar Bacillus cereus Agar 2 Dénombrement par technique NPP Enumeration by MPN protocol SURFACE / PLATING PROFONDEUR / IN DEPTH SANS CONFIRMATION / WITHOUT CONFIRMATION 3 Confirmations biochimiques Biochemical confirmations Confirmations biochimiques Biochemical confirmations MOSSEL / MYP 4 4 Confirmations biochimiques Biochemical confirmations Milieu pré-coulé / Pre-poured medium : 20 boîtes / plates Ø 90 mm Ref / Code : BM12608 Milieu prêt-à-liquéfier / Ready-to-melt medium : 10 flacons / vials 100 ml Ref / Code : BM13008 Supplément sélectif / Selective supplement : 10 flacons / vials qs 100 ml Ref / Code : BS

11 Enterobacter sakazakii Agar Recherche des Enterobacter sakazakii dans les poudres de lait infantile Detection of Enterobacter sakazakii in milk infant formulas Enterobacter sakazakii α-glucosidase (+) Spécificité Specificity α-glucosidase Lecture facile Easy reading Autres bactéries Other bacteria α-glucosidase (-) 11

12 Enterobacter sakazakii Agar Recherche des Enterobacter sakazakii Detection of Enterobacter sakazakii ISO TS Lait et Produits Laitiers Milk and Milk products ISO TS Lait et Produits Laitiers Milk and Milk products 1 Prise d essai et préenrichissement non-sélectif Sample preparation and nonselective pre-enrichment EPT / BPW Prise d essai et enrichissement sélectif Sample preparation and selective enrichment Bouillon EE EE broth 2 Enrichissement sélectif Selective enrichment m LST-v Isolement sélectif Selective isolation VRBG 3 Isolement sélectif Selective isolation Enterobacter sakazakii Agar Purification - orientation TSA Confirmation TSA Colonies pigmentées en jaune Yellow-pigmented colonies Confirmations biochimiques Biochemical confirmations 4 Milieu pré-coulé / Pre-poured medium : 20 boîtes / plates Ø 90 mm Ref / Code : BM12008 Colonies pigmentées en jaune Yellow-pigmented colonies Milieu déshydraté / Dehydrated medium : Flacons / vials 500 g Ref / Code : BK188HA 12

13 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Escherichia coli, coliforms Tryptophan Broth - Ready-to-use medium BM x3mL in tubes Tryptophan Broth BK163HA 500 g Brilliant Green Bile Broth (BGBB) single strength - Ready-to-use medium BM x10 ml with Durham tubes Brilliant Green Bile Broth (BGBB) BK002HA 500 g Laurylsulfate-Tryptose Broth BK010HA 500 g Incubation 24 hours at 37 C Characteristics Glowing tubes indicate B-glucuronidase positive bacteria Laurylsulfate-Tryptose single strength Broth - Ready-to-use medium BM X10 ml with Durham tubes Laurylsulfate-Tryptose double strength Broth - Ready-to-use medium BM X10 ml with Durham tubes MUG 50 mg supplement BS vials pack MacCONKEY Broth purple BK107HA 500 g Bromcresol Purple Lactose Broth BK119HA 500 g EC Broth BK162HA 500 g Glutamate Broth BK186HA 500 g CLED Agar BK020HA 500 g Incubation 18 hours at 37 C Characteristics Proteus : Blue, translucent colonies on blue background (or blue halo), without invasion Staphylococci : Small, opaque, yellow colonies with no halos E.coli : Large, golden yellow colonies, surrounded by yellowish halos EMB Agar (LEVINE) BK056HA 500 g ENDO Agar BK057HA 500 g Desoxycholate (0.1%) lactose Agar BK062HA 500 g Desoxycholate lactose Agar BK065HA 500 g Tergitol 7 Agar - Pre-poured medium BM plates Ø 55 mm Tergitol 7 Agar - Pre-poured medium BM plates Ø 90 mm Tergitol 7 Agar - Dehydrated base medium BK123HA 500 g TTC supplement 12.5 mg BS vials pack TBX Agar BK146HA 500 g Incubation Growth obtained after 24 hours of incubation at 44 C (pour plate inoculation) Characteristics B-glucuronidase positive Escherichia coli blue color TBX Agar BK146HM 100 g TBX Agar Ready-to-melt medium BM x200 ml in vials TBX Agar Ready-to-melt medium BM x200 ml in vials 13

14 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Escherichia coli, coliforms VRBL Agar - Ready-to-melt medium BM x100 ml in vials VRBL Agar - Ready-to-melt medium BM x100 ml in vials VRBL Agar BK152GC 5 kg VRBL Agar BK152HA 500 g MUG 50 mg supplement BS vials pack COMPASS Ecc Agar - Dehydrated medium BK202HA 500 g COMPASS cc Agar - Pre-poured medium BM plates Ø 55 mm Incubation Characteristics 24 ±hours at 36±2 C E.coli; blue-violet color Coliforms; pink color Microplate MUG/EC BT opaque microplates Escherichia coli O157 MacCONKEY-sorbitol (CT-SMAC) Agar - Dehydrated base medium BK147HA 500 g Incubation 24 hours at 37 C Characteristics E.coli O157:H7: smaller, colorless colonies sometimes with an orange halo Dyes in the agar are absorbed by the colonies, coloring them artificially E.coli non-o157:h7 : larger, red colonies. Cefixime-tellurite supplement for CT-SMAC Agar BS vials pack qsp 500 ml Modified Tryptone soy Broth (mtsb) - Dehydrated base medium BK150HA 500 g Novobiocin 10 mg selective supplement BS vials pack Total Viable Count Nutrient Broth BK003HA 500 g Non-animal Broth BK180HA 500 g Eugon Broth BK068HA 500 g Brain-Heart Broth BK015HA 500 g Thioglycollate medium with resazurin BK017GC 5 kg Thioglycollate medium with resazurin BK017HA 500 g Thioglycollate medium with resazurin - Ready-to-use medium BM x10mL in tubes Thioglycollate medium with resazurin - Ready-to-use medium BK206GC 5 kg Nutrient Agar 2 % BK185HA 500 g Nutrient Agar 2 % - Ready-to-melt medium BM x18mL in tubes Trypto-casein Soy Broth (TSB) BK046GC 5 kg Trypto-casein Soy Broth (TSB) BK046HA 500 g Trypto-casein Soy Broth (TSB) - Ready-to-use medium BM x100mL in vials Trypto-casein Soy Broth (TSB) - Ready-to-use medium BM x10mL in tubes 14

15 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Total Viable Count Trypto-casein Soy Agar (TSA) - Ready-to-melt medium BM x100 ml in vials Trypto-casein Soy Agar (TSA) - Ready-to-melt medium BM x200 ml in vials Trypto-casein Soy Agar (TSA) BK047HA 500 g Incubation Productivity After 48 hours at 32,5 C E.coli; PR %70 S.aures; PR %70 Incubation After 72 hours at C Candida albicans; PR %70 Aspergillus brasiliensis; PR %70 Sugar-Free Agar (SFA) - Ready-to-melt medium BM x100 ml in vials Sugar-Free Agar (SFA) BK126HA 500 g Count-tact Agar BK130HA 500 g Yeast extract Agar BK153HA 500 g Yeast extract Agar - Ready-to-melt medium BM x200 ml in vials Plate count Agar with skimmed milk BK161HA 500 g Plate count Agar (PCA) BK144GC 5 kg Plate count Agar (PCA) BK144HA 500 g Plate count Agar (PCA) - Ready-to-melt medium BM x200 ml in vials Plate count Agar (PCA) - Ready-to-melt medium BM x100 ml in vials Plate count Agar with skimmed milk - Ready-to-melt medium BM x200 ml in vials TTC supplement 12.5 mg BS vials pack TTC supplement 50 mg BS vials pack qsp 500 ml Lactobacillus, Leuconostoc, Lactococcus MRS Broth BK070GC 5 kg MRS Broth BK070HA 500 g MRS Broth (Non-animal) BK176HA 500 g MRS Agar BK089HA 500 g Incubation Characteristics 72 hours at 30 C (in 5 % CO2) White colonies of uniform size. Colonies found on the surface of the agar or on the very bottom will have a larger size due to the absence of agar restricting growth. MRS Agar ph Ready-to-melt medium BM x200mL in vials ROGOSA Agar BK033HA 500 g Elliker Broth BK054HA 500 g M 17 Broth BK012HA 500 g M 17 Agar BK088HA 500 g MAYEUX SANDINE ELLIKER Agar (MSE) BK087HA 500 g 15

16 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Legionella GVPC Agar for Legionella - Pre-poured medium BM plates Ø 90 mm BCYE Agar for Legionella NEN BM plates Ø 90 mm BCYE Agar for Legionella NEN BM plates Ø 90 mm Legionella Agar NEN BM plates Ø 90 mm BCYE Agar for Legionella - Pre-poured medium BM plates Ø 90 mm BCYE Agar without cysteine for Legionella - Pre-poured medium BM plates Ø 90 mm Trypto-casein Soy Agar (TSA) BK047HA 500 g Sugar-Free Agar (SFA) - Ready-to-melt medium BM x 100mL in vials Sugar-Free Agar (SFA) BK126HA 500 g Count-tact Agar BK130HA 500 g Yeast extract Agar BK153HA 500 g Yeast extract Agar - Ready-to-melt medium BM x 200mL in vials Plate count Agar with skimmed milk BK161HA 500 g Plate count Agar (PCA) BK144GC 5 kg Plate count Agar (PCA) BK144HA 500 g Plate count Agar with skimmed milk BK161HA 500 g Listeria Listeria enrichment Broth (LEB acc. to FDA) BK112HA 500 g Listeria enrichment Broth (UVM I modified) BK113HA 500 g Listeria enrichment Broth (UVM I modified) BM x5L in flexible bags Listeria enrichment Broth (LEB acc. to IDF) BK124HA 500 g FRASER Broth - Ready-to-use medium BM x10mL in tubes FRASER Broth - Dehydrated base medium BK115HA 500 g FRASER Broth (II) - Dehydrated base medium BK133GC 5 kg FRASER Broth (II) - Dehydrated base medium BK133HA 500 g Incubation 24 hours / 37 C Characteristics Blackened tube (at right) a presumptive indicaton of listeria monocytogenes growth (reduction of esculin), however corfirmation Note Half Fraser will demonstrate the same type of blackening, but in 225 ml vials. 16 Half-FRASER selective supplement BS vials pack qsp 2.25 L Half-FRASER selective supplement BS vials pack qsp 500 ml FRASER selective supplement BS vials pack qsp 500 ml Half-FRASER Broth - Ready-to-use medium BM x 225mL in vials Half-FRASER Broth - Ready-to-use medium BM x3L in flexible bags Half-FRASER Broth - Ready-to-use medium BM x5L in flexible bags Half-FRASER Broth - Dehydrated base medium BK173GC 5 kg Half-FRASER Broth - Dehydrated base medium BK173HA 500 g Sterile ferric ammonium citrate 5 % supplement BS x90mL in vials Sterile ferric ammonium citrate 5 % supplement BS x10mL in tubes Oxford Agar - Dehydrated base medium BK110HA 500 g Oxford selective supplement (CCCFA) BS vials pack qsp 500 ml

17 Listeria Agar BKR 23/02-11/02 BKR 23/05-12/07 Recherche et dénombrement des Listeria monocytogenes Detection and enumeration of Listeria monocytogenes Listeria monocytogenes β-glucosidase (+), hydrolyse du L-α-phosphatidyl-inositol β-glucosidase (+), hydrolysis of L-α-phosphatidyl-inositol Sélectivité Selectivity PI-PLC Formulation selon Ottaviani & Agosti β-glucosidase Formulation according to Ottaviani & Agosti Listeria sp. β-glucosidase (+), pas d hydrolyse du L-α-phosphatidyl-inositol β-glucosidase (+), no hydrolysis of L-α-phosphatidyl-inositol 17

18 Recherche et dénombrement des Listeria monocytogenes Detection and enumeration of Listeria monocytogenes RECHERCHE / DETECTION ISO / A1 Microbiologie des Aliments Food Microbiology Listeria Agar Certification AFNOR AFNOR certificated BKR 23/02 11/02 Microbiologie des Aliments Food Microbiology DENOMBREMENT / ENUMERATION ISO / A1 Microbiologie des Aliments Food Microbiology Certification AFNOR AFNOR certificated BKR 23/05 12/07 Microbiologie des Aliments Food Microbiology Prise d essai et pré-enrichissement non-sélectif Sample preparation and selective enrichment Prise d essai Sample preparation Fraser 1/2 Fraser 1/2 EPT/BPW - Fraser 1/2 (base)* EPT/BPW - Fraser 1/2 (base)* 1 2 Enrichissement sélectif Selective enrichment Fraser Isolement sélectif Selective isolation Listeria Agar Revivification / Resuscitation 20 C, 1h Isolement sélectif Selective isolation Listeria Agar Revivification / Resuscitation 20 C, 1h Isolement sélectif Selective isolation Listeria Agar 3 Isolement sélectif Selective isolation Listeria Agar Purification - orientation L.mono Agar Purification TSYEA Purification - orientation L.mono Agar 4 Confirmations biochimiques Biochemical confirmations * Sans ajout d agent sélectif Without selective agent 5 6 Purification TSYEA Confirmations biochimiques Biochemical confirmations Milieu pré-coulé / Pre-poured medium : 20 boîtes / plates Ø 90 mm Ref / Code : BM boîtes / plates Ø 90 mm Ref / Code : BM12408 Kit Milieu prêt-à-liquéfier / Ready-to-melt medium Supplément sélectif / Selective supplement Supplt d enrichissement / Enrichment supplt Confirm L. mono Agar Milieu pré-coulé / Pre-poured medium : Ref / Code : BT00808 : 6 flacons / vials 200 ml : 6 flacons / vials qs 200 ml : 6 flacons / vials qs 200 ml : Ref / Code : BM13908 : 10 boîtes / plates Ø 90 mm 18

19 RHAPSODY AGAR Detection and enumeration of Pseudomonas spp. in food products and environmental samples Pseudomonas spp. PERFORMANCE Detection of all Pseudomonas spp. and total inhibition of secondary flora EASY The colonies BLUE to BLUE-GREEN of Pseudomonas spp. are easily identifiable ECONOMIC Direct reading without confirmation QUICK Detection and enumeration in only 48 hours 19

20 RHAPSODY AGAR RHAPSODY Agar allows the detection and the enumeration of Pseudomonas spp. in food products and environmental samples. TO KNOW The membrane filtration method can be used for environmental samples. Incubation 48 h ± 2 H 30 ± 1 C Plating on RHAPSODY Agar RHAPSODY Agar eliminate the confirma- tion tests (culture and oxidase tests on five colonies) specified in the reference methods NF EN ISO : Meat and meat products and in ISO/TS : Milk and milk products : RHAPSODY Agar NF EN ISO 13720:2010 Meat and meat products D+2 ISO/TS 11059:2009 Milk and milk products D+2 Reading WITHOUT CONFIRMATION Confirmation D+4/D+5 Enumeration of Pseudomonas spp. colonies BLUE to BLUE-GREEN ORDERING INFORMATION RHAPSODY Agar BM Plates (Ø 90 mm) 20

21 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Listeria TSYE Agar BK171HA 500 g TSYE Agar - Ready-to-melt medium BM x18mL in tubes COMPASS Listeria Agar - Pre-poured medium BK192HA 500 g COMPASS Listeria Agar - Enrichment supplement - liquid BS vials pack qsp 1L COMPASS Listeria Agar - Selective supplement - lyophilised BS vials pack qsp 1L COMPASS Listeria Agar - Pre-poured medium BM plates Ø 90 mm COMPASS Listeria Agar - Pre-poured medium BM plates Ø 90 mm COMPASS Listeria Agar BT x200 ml base medium in vials + 6 vials of selective suppl. qsp 200 ml m + 6 vials of enrichment suppl. qsp 200 ml CONFIRM L. mono Agar - Pre-poured medium BM plates Ø 90 mm CONFIRM L. mono Broth BM x1mL in vials PALCAM Agar - Pre-poured medium BM plates Ø 90 mm PALCAM Agar - Dehydrated base medium BK145HA 500 g PALCAM selective supplement BS vials pack qsp 500 ml PALCAM selective supplement BS vials pack qsp 2.5 L Pseudomonas Acetamide Broth - Ready-to-use BM x 5mL in tubes Cetrimide Agar - Dehydrated base medium (without glycerol) BK049HA 500 g CFC Agar - Ready-to-melt base medium BM x 200mL in vials CFC Agar - Dehydrated base medium BK118HA 500 g CFC selective supplement BS vials pack qsp 500 ml Glucose Agar - Ready-to-use medium BM x 10mL in tubes KING B Agar - Ready-to-melt medium BM x 7mL in tubes CN Agar for Pseudomonas - Dehydrated base medium (with Cetrimide and BK165HA 500 g Nalidixic acid without glycerol) CN Agar for Pseudomonas - Pre-poured medium BM plates Ø 55 mm Incubation 4 hours / 36 C Characteristics Yellow green colonies characterizing pyocyanin production, or presenting a fluorescence under UV light at 360 nm are considered to be pseudomonas aeruginosa Penicillin Pimaricine Agar (PPA) - Pre-poured medium BM plates Ø 90 mm RHAPSODY Agar - Pre-poured medium BM boîtes Ø 90 mm Salmonella, Shigella WILSON-BLAIR modified BK004HA 500 g Selenite-Cystine Broth BK009HA 500 g RAPPAPORT-VASSILIADIS Soja (RVS) Broth BK148HA 500 g RAPPAPORT-VASSILIADIS Soja (RVS) Broth - Ready-to-use medium BM x10mL in tubes 21

22 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Salmonella, Shigella MÜLLER-KAUFFMANN Broth - Dehydrated base medium BK135HA 500 g Novobiocin 10 mg selective supplement BS vials pack Novobiocin 40 mg selective supplement BS vials pack MKTTn Broth - Dehydrated base medium with novobiocin BK208HA 500 g MKTTn Broth - Ready-to-use medium BM x10mL in tubes MKTTn Broth - Dehydrated base medium BK169HA 500 g Novobiocin 10 mg selective supplement BS vials pack Novobiocin 40 mg selective supplement BS vials pack MSRV Agar (ISO 6579) BK191HA 500 g Incubation 24 hours / 41,5 C Characteristics Salmonella : white, opaque jalo centered arround the point of inoculation; Non-Salmonella enteric bacteria : no opaque halo; if growth, only centered on the inoculation point. 22 MSRV Agar (ISO 6579) - Ready-to-melt medium BM x200mL in vials MSRV Agar - Dehydrated base medium BK134HA 500 g Novobiocin 10 mg selective supplement BS vials pack Novobiocin 40 mg selective supplement BS vials pack XLD Agar BK058HA 500 g XLD Agar (ISO 6579) - Pre-poured medium BM plates Ø 90 mm XLD Agar (ISO 6579) BK168HA 500 g SS Agar BK022HA 500 g Hektoen Enteric Agar BK067HA 500 g MacCONKEY Agar BK050HA 500 g MUG 50 mg supplement BS vials pack Brilliant Green Agar (KRISTENSEN) BK071HA 500 g Brilliant Green Agar (EDEL and KAMPELMACHER) BK091HA 500 g XLT4 Agar - Pre-poured medium BM plates Ø 90 mm XLT4 Agar - Dehydrated base medium BK156HA 500 g Tergitol 4 selective supplement BS ml in vial SESAME Salmonella Detection - Ready-to-melt medium BM x200mL in vials SESAME Salmonella Detection - Pre-poured medium BM plates Ø 90 mm SESAME Salmonella Detection - Pre-poured medium BM plates Ø 90 mm COMPASS Salmonella Agar - Pre-poured medium BM plates Ø 90 mm Salmonella Enrichment BK194GC 5 kg Salmonella Enrichment BK194HA 500 g Salmonella Enrichment - Ready-to-use medium BM x225mL in vials Salmonella Enrichment - Ready-to-use medium BM x3L in flexible bags Salmonella Enrichment - Ready-to-use medium BM x5L in flexible bags Salmonella Enrichment with Tween 80 - Ready-to-use medium BM x3L in flexible bags

23 Salmonella Agar BKR 23/04-12/07 Recherche et confirmation des salmonelles Detection and confirmation of Salmonella Différenciation Differenciation Sélectivité Selectivity Salmonella sp. esterase (+) β-glucosidase (-) C8-esterase Autres bactéries Other bacteria esterase (+/-) β-glucosidase (+) Récupération des salmonelles atypiques Recovery of atypical Salmonella lactose (+) saccharose (+) H2S(-) propylene glycol (-) lysine decarboxylase (-) glucuronate(-) 21 23

24 Salmonella Agar Recherche des salmonelles / Detection of Salmonella ISO 6579 Microbiologie des Aliments Food Microbiology ISO 6579/A1 Productions Primaires Primary Productions ISO 6785 Lait et Produits Laitiers Milk and Milk products NFU47-100,101,102 Santé Animale Animal Health Prise d essai / Sample preparation Suspension mère / Initial suspension Pré-enrichissement non sélectif / Non-selective pre-enrichment 1 EPT / BPW EPT / BPW EPT / BPW EPT / BPW Pré-enrichissement sélectif / Selective pre-enrichment 3 2 RVS + MKTTn MSRV RVS + Selenite-cystine MSRV / MKTTn / Selenite-cystine Isolement sélectif / Selective isolation XLD Hektoen Edel & Kampelmacher XLD / XLT4 / Hektoen 4 lactose (+), H2S(-) lactose (-), H2S(-) saccharose (+) lactose (+), H2S(+) Risques de faux-négatifs dans le cas de salmonelles atypiques / Risks of false-negatives in the event of atypical Salmonella Salmonella Agar Récupération des salmonelles atypiques Recovery of atypical Salmonella Purification, confirmations biochimiques et sérologiques / Purification, biochimical and serological confirmations 6 5 Milieu pré-coulé / Pre-poured medium 20 boîtes / plates Ø 90 mm Ref / Code : BM06608 Salmonella Agar est aussi utilisé comme milieu de confirmation dans la méthodecertifiéeparafnorvalidationsesamesalmonellatest (BKR23/4-12/07) Compass Salmonella Agar is also used as confirmation medium in the AFNOR Validation certified method SESAME Salmonella TEST (BKR 23/4-12/07) 24

25 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Salmonella, Shigella IRIS Salmonella supplement - Tablets BS tablets IRIS Salmonella supplement - Liquid BS x50mL in vials IRIS Salmonella Agar - Pre-poured medium BM plates Ø 90 mm IRIS Salmonella Agar - Pre-poured medium BM plates Ø 90 mm CONFIRM Salmonella - Latex agglutination kit BT tests Staphylococcus GIOLITTI and CANTONI with Tween 80 single strength Broth - Ready-to-use medium BM x10mL in tubes GIOLITTI and CANTONI with Tween 80 double strength Broth - Ready-to-use medium BM x10mL in tubes GIOLITTI and CANTONI Broth with Tween 80 - Dehydrated base medium BK159HA 500 g BUTTIAUX-BROGNIART hypersalted Broth (double strength) BK081HA 500 g Mannitol salt Agar BK030HA 500 g Mannitol salt Agar - Pre-poured medium BM plates Ø 55 mm BAIRD-PARKER Agar - Dehydrated base medium BK055GC 5 kg BAIRD-PARKER Agar - Dehydrated base medium BK055HA 500 g Egg Yolk Enrichment BS x50mL in vials Sterile Egg Yolk Tellurite Enrichment BS x50mL in vials Sulfamethazine 25 mg selective supplement BS vials pack qsp 500 ml Rabbit Plasma Fibrinogen (RPF) supplement BS vials pack (1 vial qsp 100 ml) Rabbit Plasma Fibrinogen (RPF) supplement BS vial qsp 500 ml BAIRD PARKER Agar with Egg Yolk and Tellurite - Pre-poured medium BM plates Ø 90 mm BAIRD PARKER Agar with Egg Yolk and Tellurite - Pre-poured medium BM plates Ø 90 mm BAIRD-PARKER RPF Agar - Pre-poured medium BM plates Ø 90 mm BAIRD-PARKER RPF Agar BT x90 ml vials of base medium ready-to-melt + 6 vials of RPF suppl. qsp 100 ml BAIRD-PARKER RPF Agar BT x180 ml vials of base medium ready-to-melt + 6 vials of RPF suppl. qsp 200 ml BAIRD-PARKER RPF Agar - Pre-poured medium BM plates Ø 55 mm Incubation Characteristics 24 hours / 37 C (surface) Coagulase positive staphylococci are gray to black surrounded by a halo of fibrin. (reduction of tellurite and plasma coagulation) Staphylococcus Characteristics Colonies Gray to black colony surrounded by an opaque halo of fibrin BAIRD PARKER Agar with Egg Yolk and Tellurite - Pre-poured medium BM plates Ø 90 mm Brain-Heart Broth BK015HA 500 g Lyophilized coagulase rabbit plasma BR vials pack for 20 reactions 25

26 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Streptococcus, Enterococcus Ethyl violet azide (LITSKY) Broth BK061HA 500 g Azide Dextrose Broth (acc. to ROTHE) BK060HA 500 g SLANETZ and BARTLEY Agar - Pre-poured medium BM plates Ø 55 mm SLANETZ and BARTLEY Agar - Pre-poured medium BM plates Ø 55 mm SLANETZ and BARTLEY Agar BK037HA 500 g SLANETZ and BARTLEY Agar - Dehydrated base medium BK129HA 500 g TTC supplement 50 mg BS vials pack qsp 500 ml KF Streptococcus Agar - Dehydrated base medium BK132HA 500 g Right: Enterococcus faecalis Membrane filtration technique (water testing) 24 hours / 37 C Red to maroon colonies (TTC reduction) surrounded by a yellow halo under the membrane or in the agar, depending onthe technique 26 TTC supplement 50 mg BS vials pack qsp 500 ml BEA Agar - Ready-to-melt medium BM x100mL in vials BEA Agar BK158HA 500 g COMPASS Enterococcus Agar BK183HA 500 g COMPASS Enterococcus Agar - Ready-to-melt medium BM x100mL in vials COMPASS Enterococcus Agar - Ready-to-melt medium BM plates Ø 55 mm Microplate MUD/SF BT opaque microplates Vibrio TCBS Agar BK040HA 500 g Yeasts and molds Non-animal malt extract broth BK205GC 5 kg Non-animal malt extract broth BK205HA 500 g Sabouraud Dextrose Broth BK026HA 500 g Malt Agar BK045HA 500 g Orange Serum Agar (OSA) BK103HA 500 g Wort Agar - Dehydrated base medium BK013HA 500 g Sabouraud Dextrose Agar BK025HA 500 g Gentamicin 25 mg selective supplement BS vials pack Chloramphenicol 50 mg selective supplement BS vials pack Sabouraud Dextrose Chloramphenicol Agar (SDCA) - Pre-poured medium BM plates Ø 90 mm Sabouraud Chloramphenicol Agar BK027HA 500 g Oxytetracycline glucose Agar (OGA) - Ready-to-melt base medium BM x110mL in vials Oxytetracycline glucose Agar (OGA) BK053HA 500 g Oxytetracycline 50 mg selective supplement BS vials pack qsp 550 ml Potato Dextrose Agar (PDA) BK095HA 500 g

27 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Yeasts and molds Rose Bengal Chloramphenicol Agar BK151HA 500 g Dichloran-Glycerol (DG18) Agar - Dehydrated base medium BK170HA 500 g Dichloran-Glycerol (DG18) Agar - Ready-to-melt medium BM x100mL in vials Ethyl violet azide (LITSKY) Broth BK061HA 500 g Incubation 2-5 days at 25 C Characteristics Yeast; pinkish colonies Dichloran Rose Bengal Chloramphenicol (DRBC) Agar - Ready-to-melt medium BM x200mL in vials Chloramphenicol glucose Agar BK007HA 500 g Chloramphenicol glucose Agar - Ready-to-melt medium BM x100mL in vials Chloramphenicol glucose Agar - Ready-to-melt medium BM x200mL in vials Gentamicin 25 mg selective supplement BS vials pack Antibiotic susceptibility testing MUELLER-HINTON Broth BK108HA 500 g MUELLER-HINTON Agar BK048HA 500 g WILKINS-CHALGREN Agar BK101HA 500 g Blood agar base Columbia Agar - Dehydrated base medium BK019HA 500 g Tryptone-Soy Agar (Blood Agar base) BK028HA 500 g Brain-Heart Agar BK029HA 500 g Culture media base Bacteriological Agar type A A1010GC 5 kg Bacteriological Agar type A A1010HA 500 g Bacteriological Agar type E A1012GC 5 kg Bacteriological Agar type E A1012HA 500 g Bacteriological malt extract A1101GC 5 kg Bacteriological malt extract A1101HA 500 g Yeast extract A1202GC 5 kg Yeast extract A1202HA 500 g Tryptone USP A1401GC 5 kg Tryptone USP A1401HA 500 g Pancreatic Hydrolysate of Casein Codex A1402GC 5 kg Pancreatic Hydrolysate of Casein Codex A1402HA 500 g Acid Hydrolysate of Casein A1404HA 500 g Papaic digest of soybean meal USP A1601HA 500 g 27

28 Enterococcus Agar Recherche et dénombrement des des enterocoques Detection and enumeration of enterococci Sans confirmation Without confirmation Enterococcus Filtration sur membrane / Membrane fiiltration Résultat en 24h Result in 24h Autres bactéries Other bacteria β-glucosidase Sélectivité Selectivity 28

29 Recherche et dénombrement des enterocoques Detection and enumeration of enterococci FIL 149A Lait et Produits Laitiers Milk and Milk products Enterococcus Agar Enterococcus Agar Lait et Produits Laitiers Milk and Milk products ISO T Eaux destinées à la consommation humaine Drinking water Enterococcus Agar Eaux destinées à la consommation humaine Drinking water Prise d essai / Sample preparation Tryptone-sel Tryptone-salt Tryptone-sel Tryptone-salt Prise d essai / Sample preparation Filtration sur membrane Membrane filtration Filtration sur membrane Membrane filtration 1 Enterococcus Agar Enterococcus Agar KF Slanetz & Bartley 2 SANS CONFIRMATION WITHOUT CONFIRMATION Confirmation BEA 3 Milieu prêt-à-liquéfier / Ready-to-melt medium : 10 flacons / vials 100ml Ref / Code : BM11608 Milieu déshydraté / Dehydrated medium : Flacons / vials 500 g Ref / Code : BK183HA 29

30 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Culture media base Pancreatic digest of meat type 2 A1702HA 500 g Peptic digest of meat USP A1708HA 500 g Bacteriological meat extract A1710HA 500 g Accessory Stainless steel connector for flexible bags BA00208 Unit Water range Sterile distilled water BM x18mL in tubes Acetamide Broth - Ready-to-use BM x5mL in tubes Bromcresol Purple Lactose Broth BK119HA 500 g Laurylsulfate-Tryptose Broth BK010HA 500 g Laurylsulfate-Tryptose single strength Broth - Ready-to-use medium BM x10 ml with Durham tubes Laurylsulfate-Tryptose double strength Broth - Ready-to-use medium BM x10 ml with Durham tubes Brilliant Green Bile Broth (BGBB) BK002HA 500 g Brilliant Green Bile Broth (BGBB) single strength - Ready-to-use medium BM x10 ml with Durham tubes MKTTn Broth - Ready-to-use medium BM x10mL in tubes Tryptophan Broth BK163HA 500 g Incubation 24 hours / 37 C (NF EN ISO 16654) Characteristics Indole production in E. coli is characterized by a red ring on the surface of the broth after addition of Kovac s reagent. Negative indole remains colorless to yellow. Tryptophan Broth - Ready-to-use medium BM x3mL in tubes Novobiocin 10 mg selective supplement BS vials pack Novobiocin 40 mg selective supplement BS vials pack RAPPAPORT-VASSILIADIS Soja (RVS) Broth - Ready-to-use medium BM x10mL in tubes BAIRD-PARKER Agar - Dehydrated base medium BK055HA 500 g BAIRD-PARKER Agar - Dehydrated base medium BK055GC 5 kg Rabbit Plasma Fibrinogen (RPF) supplement BS vials pack (1 vial qsp 100 ml) Rabbit Plasma Fibrinogen (RPF) supplement BS vial qsp 500 ml BAIRD-PARKER RPF Agar - Pre-poured medium BM plates Ø 90 mm BAIRD-PARKER RPF Agar - Pre-poured medium BM plates Ø 55 mm BAIRD-PARKER RPF Agar BT x90 ml vials of base medium ready-to-melt + 6 vials of RPF suppl. qsp 100 ml BAIRD-PARKER RPF Agar BT x180 ml vials of base medium ready-to-melt + 6 vials of RPF suppl. qsp 200 ml BAIRD PARKER Agar with Egg Yolk and Tellurite - Pre-poured medium BM plates Ø 90 mm BAIRD PARKER Agar with Egg Yolk and Tellurite - Pre-poured medium BM plates Ø 90 mm 30

31 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Water range BEA Agar BK158HA 500 g BEA Agar - Ready-to-melt medium BM x100mL in vials BCYE Agar for Legionella NEN BM plates Ø 90 mm BCYE Agar for Legionella NEN BM plates Ø 90 mm Mannitol salt Agar BK030HA 500 g Mannitol salt Agar - Pre-poured medium BM plates Ø 55 mm CN Agar for Pseudomonas - Dehydrated base medium (with Cetrimide and BK165HA 500 g Nalidixic acid without glycerol) CN Agar for Pseudomonas - Pre-poured medium BM plates Ø 55 mm Meat-liver glucose Agar BK157HA 500 g GVPC Agar for Legionella - Pre-poured medium BM plates Ø 90 mm KING B Agar - Ready-to-melt medium BM x7mL in tubes Bromcresol Purple Lactose Agar BK023HA 500 g Tergitol 7 Agar - Dehydrated base medium BK123HA 500 g Tergitol 7 Agar - Pre-poured medium BM plates Ø 55 mm Incubation 48 hours / 37 C Characteristics Yellow-orange colonies on amber agar (colony color due to fermentation of lactose, no TTC reduction) Tergitol 7 Agar - Pre-poured medium BM plates Ø 55 mm Yeast extract Agar BK153HA 500 g Yeast extract Agar - Ready-to-melt medium BM x200mL in vials Microplate MUG/EC BT opaque microplates Description Microplate MUG/EC allows the practical identification and enumeration of Escherichia coli in water, according to ISO standard NF EN ISO Microplate MUD/SF BT opaque microplates Nutrient Agar 2.5 % - Ready-to-melt medium BM x18mL in tubes Lyophilized coagulase rabbit plasma BR vials pack for 20 reactions Synthetic sea salt BR g Synthetic sea salt with Bromphenol blue - Ready-to-use medium BM x18mL in tubes SLANETZ and BARTLEY Agar - Dehydrated base medium BK129HA 500 g SLANETZ and BARTLEY Agar BK037HA 500 g SLANETZ and BARTLEY Agar - Pre-poured medium BM plates Ø 55 mm Incubation 48 hours / 37 C Characteristics Red, maroon or pink colonies (due to TTC reduction) on membranes Enterococcus faecalis; pink to red-brown colonies 31

32 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Water range SLANETZ and BARTLEY Agar - Pre-poured medium BM plates Ø 55 mm Ringer s solution (1/4 strength) BR tablets Sulfamethazine 25 mg selective supplement BS vials pack qsp 500 ml Tryptone-Soy Agar (Blood Agar base) BK028HA 500 g TSC Agar - Ready-to-use base medium BM g TSC Agar - Ready-to-melt base medium BM x20mL in tubes TSC Agar - Ready-to-melt base medium BM x200mL in vials TSI Agar BK059HA 500 g TTC supplement 12.5 mg BS vials pack TTC supplement 50 mg BS vials pack qsp 500 ml XLD Agar (ISO 6579) BK168HA 500 g XLD Agar (ISO 6579) - Pre-poured medium BM plates Ø 90 mm IRIS Salmonella Agar - Pre-poured medium BM plates Ø 90 mm IRIS Salmonella Agar - Pre-poured medium BM plates Ø 90 mm COMPASS Salmonella Agar - Pre-poured medium BM plates Ø 90 mm COMPASS cc Agar - Pre-poured medium BM plates Ø 55 mm COMPASS cc Agar - base BK210HA 500 g COMPASS cc Agar - Selective supplement BS vials pack qsp 500 ml COMPASS Enterococcus Agar BK183HA 500 g COMPASS Enterococcus Agar - Ready-to-melt medium BM x100mL in vials COMPASS Enterococcus Agar - Pre-poured medium BM plates Ø 55 mm Cosmetics range Peptone water 0.1 % BM x5L in flexible bags Tryptone-salt Broth (Maximum Recovery Diluent) - Ready-to-use medium BM x9mL in tubes Tryptone-salt Broth (Maximum Recovery Diluent) - Ready-to-use medium BM x90mL in vials Tryptone-salt Broth (Maximum Recovery Diluent) - Ready-to-use medium BM x3L in flexible bags Trypto-casein Soy Broth (TSB) - Ready-to-use medium BM x10mL in tubes Trypto-casein Soy Broth (TSB) - Ready-to-use medium BM x100mL in vials Trypto-casein Soy Broth (TSB) - Ready-to-use medium BM x90mL in vials Trypto-casein Soy Broth (TSB) - Ready-to-use medium BM x5L in flexible bags Lactose Broth BK082HA 500 g Eugon LT 100 Broth - Ready-to-use medium BM x9mL in tubes Eugon LT 100 Broth - Ready-to-use medium BM x9mL in tubes with glasses beads Eugon LT 100 Broth - Ready-to-use medium BM x100mL in vials Eugon LT 100 Agar - Pre-poured medium BM plates Ø 90 mm Eugon LT 100 Agar - Ready-to-melt medium BM x200mL in vials 32

33 CLASSIFICATION - BY MICROORGANISMS Designation Code Packaging Cosmetics range BAIRD-PARKER Agar - Dehydrated base medium BK055HA 500 g BAIRD-PARKER Agar - Dehydrated base medium BK055GC 5 kg Sterile Egg Yolk Tellurite Enrichment BS x50mL in vials BAIRD PARKER Agar with Egg Yolk and Tellurite - Pre-poured medium BM plates Ø 90 mm BAIRD PARKER Agar with Egg Yolk and Tellurite - Pre-poured medium BM plates Ø 90 mm Cetrimide Agar - Dehydrated base medium (without glycerol) BK049HA 500 g Mannitol salt Agar BK030HA 500 g EMB Agar (LEVINE) BK056HA 500 g Potato Dextrose Agar (PDA) BK095HA 500 g Chloramphenicol 50 mg selective supplement BS vials pack MacCONKEY Agar - ready-to-melt medium BM x100mL in vials Sabouraud Dextrose Agar (SDA) - Pre-poured medium BM plates Ø 90 mm Sabouraud Dextrose Agar (SDA) - Pre-poured medium BM plates Ø 90 mm Sabouraud Dextrose Agar (SDA) - Ready-to-melt medium BM x200mL in vials Sabouraud Dextrose Chloramphenicol Agar (SDCA) - Ready-to-melt medium BM plates Ø 90 mm Sabouraud Dextrose Chloramphenicol Agar (SDCA) - Ready-to-melt medium BM x200mL in vials Sabouraud Dextrose Chloramphenicol Agar (SDCA) - Ready-to-melt medium BM plates Ø 90 mm Sabouraud Dextrose Agar BK025HA 500 g Gentamicin 25 mg selective supplement BS vials pack Chloramphenicol 50 mg selective supplement BS vials pack Trypto-casein Soy Agar (TSA) - Pre-poured medium BM plates Ø 90 mm Trypto-casein Soy Agar (TSA) - Pre-poured medium BM plates Ø 90 mm Trypto-casein Soy Agar (TSA) - Ready-to-melt medium BM x100mL in vials Trypto-casein Soy Agar (TSA) - Ready-to-melt medium BM x200mL in vials 33

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BIOKAR Diagnostics customer services Alternative methods validated by AFNOR Certification Rapid chromogenic methods...

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