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1 Table 1: Results of fish collections within or adjacent to the Boyne Secondary Plan Area conducted by C. Portt and Associates. Station locations are provided in Figure 1. Stations BA1-BA6 were collected during the Bristol Survey audit in Stations CP1-CP11 were collected during the present study in Station BA1 BA2 BA3 BA4 BA5 BA6 CP1 CP2 CP3 CP4 CP5 CP6 Date (d/m/y) 19/7/05 19/7/05 19/7/05 19/7/05 19/7/05 19/7/05 18/4/08 18/4/08 15/4/08 18/4/08 18/4/08 7/7/08 Number of fish species collected pumpkinseed (Lepomis gibbosus) fathead minnow (Pimephales promelas) 4 YOY YOY 45 YOY 37 YOY brook stickleback (Culaea inconstans) 1 1 creek chub (Semotilus atromaculatus) 1 2 goldfish (Carassius auratus) 1 largemouth bass (Micropterus salmoides) 1 white sucker (Catostomus commersonii) YOY 13 bluntnose minnow (Pimephales notatus) Station CP7 CP10 CP11 Date (d/m/y) 7/7/08 22/10/08 22/10/08 Number of fish species collected pumpkinseed (Lepomis gibbosus) fathead minnow (Pimephales promelas) 2 brook stickleback (Culaea inconstans) 1 creek chub (Semotilus atromaculatus) 1 goldfish (Carassius auratus) largemouth bass (Micropterus salmoides) white sucker (Catostomus commersonii) YOY 1 2 bluntnose minnow (Pimephales notatus) 3

2 Table 2: Results of fish collections within or adjacent to the Boyne Secondary Plan Area conducted by LGL Limited in 2007 and Station locations are provided in Figure 1. For stations at which two collection attempts were made, but fish were found on only one attempt, the date on which fish were collected is indicated Station LGL1 LGL2 LGL3 LGL4 LGL5 LGL6 LGL7 LGL8 LGL9 Dates (d/m/y) 6/6/07 23/4/08 6/6/07 23/4/08 6/6/07 23/4/08 23/4/08 23/4/08 23/4/08 23/4/08 Number of fish species collected pumpkinseed (Lepomis gibbosus) fathead minnow (Pimephales promelas) brook stickleback (Culaea inconstans) creek chub (Semotilus atromaculatus) 2008 goldfish (Carassius auratus) largemouth bass (Micropterus salmoides) white sucker (Catostomus commersonii) Station LGL10 LGL11 LGL12 LGL13 LGL14 LGL15 LGL16 LGL17 LGL18 Date (d/m/y) 6/6/07 6/6/07 6/6/07 6/6/07 6/6/07 6/6/07 6/6/07 23/4/08 Number of fish species collected pumpkinseed (Lepomis gibbosus) fathead minnow (Pimephales promelas) 2008 brook stickleback (Culaea inconstans) 2008 creek chub (Semotilus atromaculatus) 2008 goldfish (Carassius auratus) largemouth bass (Micropterus salmoides) white sucker (Catostomus commersonii)

3 Table 3: Fish collection records held by Conservation Halton for portions of Sixteen Mile Creek Subwatersheds 2 and 7 (S-n) and Indian Creek subwatershed in Bronte Creek (B-n), within or downstream of the Boyne Secondary Plan Area. Station locations are provided in Figure 2. Station B-35 B-36 B-37 B-37 B-97 S-25 S-25 S-25 S-26 S-26 S-26 S-35 Date (d/m/y) 20/11/01 20/11/01 16/8/79 20/11/01 20/11/01 4/7/ /5/99 4/7/ /5/99 25/6/73 Number of fish species collected alewife (Alosa pseudoharengus) 1 black crappie (Pomoxis nigromaculatus) blacknose dace (Rhinichthys atratulus) bluntnose minnow (Pimephales notatus) brook stickleback (Culaea inconstans) carps and minnows (Cyprinidae) 1 common shiner (Luxilus cornutus) creek chub (Semotilus atromaculatus) emerald shiner (Notropis atherinoides) fantail darter (Etheostoma flabellare) 1 1 fathead minnow (Pimephales promelas) 1 8 golden redhorse (Moxostoma erythrurum) johnny darter (Etheostoma nigrum) 1 1 largemouth bass (Micropterus salmoides) longnose dace (Rhinichthys cataractae) river or hornyhead chub (Nocomis sp.) 27 7 northern hog sucker (Hypentelium nigricans) 2 5 northern pike (Esox lucius) pumpkinseed (Lepomis gibbosus) 1 rainbow darter (Etheostoma caeruleum) rainbow trout (Oncorhynchus mykiss) river chub (Nocomis micropogon) 11 rock bass (Ambloplites rupestris) rosyface shiner 6 sea lamprey (Petromyzon marinus) smallmouth bass (Micropterus dolomieu) spottail shiner (Notropis hudsonius) stonecat (Noturus flavus) 1 white sucker (Catostomus commersonii) yellow bullhead (Ameiurus natalis)

4 Table 3 (cont.): Fish collection records held by Conservation Halton for portions of Sixteen Mile Creek Subwatersheds 2 and 7 (S-n) and Indian Creek subwatershed in Bronte Creek (B-n), within or downstream of the Boyne Secondary Plan Area. Station locations are provided in Figure 2. Station S-35 S-38 S-38 S-67 S-68 S-68 S-68 S-69 S-69 S-75 S-79 S-79 Date (d/m/y) 22/8/84 22/6/73 15/8/05 11/6/ /6/75 14/8/98 5/8/98 6/6/ /8/ Number of fish species collected alewife (Alosa pseudoharengus) black crappie (Pomoxis nigromaculatus) blacknose dace (Rhinichthys atratulus) 2 bluntnose minnow (Pimephales notatus) brook stickleback (Culaea inconstans) 2 carps and minnows (Cyprinidae) 1 common shiner (Luxilus cornutus) 7 27 creek chub (Semotilus atromaculatus) 4 emerald shiner (Notropis atherinoides) fantail darter (Etheostoma flabellare) 3 fathead minnow (Pimephales promelas) 37 golden redhorse (Moxostoma erythrurum) 4 johnny darter (Etheostoma nigrum) largemouth bass (Micropterus salmoides) 2 2 longnose dace (Rhinichthys cataractae) 2 6 river or hornyhead chub (Nocomis sp.) northern hog sucker (Hypentelium nigricans) 8 northern pike (Esox lucius) 1 pumpkinseed (Lepomis gibbosus) 16 5 rainbow darter (Etheostoma caeruleum) rainbow trout (Oncorhynchus mykiss) river chub (Nocomis micropogon) 11 rock bass (Ambloplites rupestris) rosyface shiner 1 sea lamprey (Petromyzon marinus) smallmouth bass (Micropterus dolomieu) 1 1 spottail shiner (Notropis hudsonius) stonecat (Noturus flavus) 1 1 white sucker (Catostomus commersonii) yellow bullhead (Ameiurus natalis)

5 Table 3 (cont.): Fish collection records held by Conservation Halton for portions of Sixteen Mile Creek Subwatersheds 2 and 7 (S-n) and Indian Creek subwatershed in Bronte Creek (B-n), within or downstream of the Boyne Secondary Plan Area. Station locations are provided in Figure 2. Station S-103 S-110 S-110 S-110 S-112 S-115 S-132 S-133 S-134 S-141 S-149 S-155 Date (d/m/y) 16/8/ /9/93 11/8/05 14/9/93 14/9/93 17/5/ /5/ /12/84 8/9/05 Number of fish species collected alewife (Alosa pseudoharengus) black crappie (Pomoxis nigromaculatus) blacknose dace (Rhinichthys atratulus) 1 bluntnose minnow (Pimephales notatus) brook stickleback (Culaea inconstans) 8 carps and minnows (Cyprinidae) 1 common shiner (Luxilus cornutus) 7 creek chub (Semotilus atromaculatus) emerald shiner (Notropis atherinoides) 19 fantail darter (Etheostoma flabellare) 17 fathead minnow (Pimephales promelas) golden redhorse (Moxostoma erythrurum) johnny darter (Etheostoma nigrum) 3 largemouth bass (Micropterus salmoides) 2 longnose dace (Rhinichthys cataractae) 87 river or hornyhead chub (Nocomis sp.) northern hog sucker (Hypentelium nigricans) northern pike (Esox lucius) pumpkinseed (Lepomis gibbosus) rainbow darter (Etheostoma caeruleum) rainbow trout (Oncorhynchus mykiss) river chub (Nocomis micropogon) 8 3 rock bass (Ambloplites rupestris) 2 25 rosyface shiner sea lamprey (Petromyzon marinus) smallmouth bass (Micropterus dolomieu) 1 3 spottail shiner (Notropis hudsonius) stonecat (Noturus flavus) white sucker (Catostomus commersonii) yellow bullhead (Ameiurus natalis)

6 Table 3 (cont.): Fish collection records held by Conservation Halton for portions of Sixteen Mile Creek Subwatersheds 2 and 7 (S-n) and Indian Creek subwatershed in Bronte Creek (B-n), within or downstream of the Boyne Secondary Plan Area. Station locations are provided in Figure 2. Station S-158 S-155 S-158 S-163 S-164 S-165 S-166 S-172 S-178 S-205 S-205 S-216 Date (d/m/y) 30/7/98 8/9/05 30/7/98 29/7/98 29/7/98 29/7/98 29/7/98 9/10/05 29/7/98 19/7/06 3/7/07 14/8/98 Number of fish species collected alewife (Alosa pseudoharengus) black crappie (Pomoxis nigromaculatus) 1 blacknose dace (Rhinichthys atratulus) bluntnose minnow (Pimephales notatus) brook stickleback (Culaea inconstans) 7 carps and minnows (Cyprinidae) 10 common shiner (Luxilus cornutus) creek chub (Semotilus atromaculatus) emerald shiner (Notropis atherinoides) fantail darter (Etheostoma flabellare) fathead minnow (Pimephales promelas) golden redhorse (Moxostoma erythrurum) johnny darter (Etheostoma nigrum) largemouth bass (Micropterus salmoides) 9 longnose dace (Rhinichthys cataractae) river or hornyhead chub (Nocomis sp.) northern hog sucker (Hypentelium nigricans) northern pike (Esox lucius) pumpkinseed (Lepomis gibbosus) rainbow darter (Etheostoma caeruleum) rainbow trout (Oncorhynchus mykiss) river chub (Nocomis micropogon) rock bass (Ambloplites rupestris) rosyface shiner 4 4 sea lamprey (Petromyzon marinus) smallmouth bass (Micropterus dolomieu) spottail shiner (Notropis hudsonius) stonecat (Noturus flavus) white sucker (Catostomus commersonii) yellow bullhead (Ameiurus natalis)

7 Table 3 (cont.): Fish collection records held by Conservation Halton for portions of Sixteen Mile Creek Subwatersheds 2 and 7 (S-n) and Indian Creek subwatershed in Bronte Creek (B-n), within or downstream of the Boyne Secondary Plan Area. Station locations are provided in Figure 2. Station S-216 S-216 S-216 S-217 S-218 S-239 S-240 S-241 S-242 S-254 S-395 S-412 Date (d/m/y) 11/8/05 31/7/06 4/7/07 14/8/98 14/8/98 14/8/98 14/8/98 14/8/98 14/8/98 26/6/01 17/6/03 25/6/04 Number of fish species collected alewife (Alosa pseudoharengus) black crappie (Pomoxis nigromaculatus) blacknose dace (Rhinichthys atratulus) 7 4 bluntnose minnow (Pimephales notatus) brook stickleback (Culaea inconstans) 6 carps and minnows (Cyprinidae) 56 common shiner (Luxilus cornutus) 1 2 creek chub (Semotilus atromaculatus) emerald shiner (Notropis atherinoides) fantail darter (Etheostoma flabellare) 1 5 fathead minnow (Pimephales promelas) golden redhorse (Moxostoma erythrurum) johnny darter (Etheostoma nigrum) largemouth bass (Micropterus salmoides) longnose dace (Rhinichthys cataractae) river or hornyhead chub (Nocomis sp.) northern hog sucker (Hypentelium nigricans) northern pike (Esox lucius) pumpkinseed (Lepomis gibbosus) rainbow darter (Etheostoma caeruleum) rainbow trout (Oncorhynchus mykiss) 1 river chub (Nocomis micropogon) rock bass (Ambloplites rupestris) 2 rosyface shiner sea lamprey (Petromyzon marinus) smallmouth bass (Micropterus dolomieu) 2 spottail shiner (Notropis hudsonius) stonecat (Noturus flavus) white sucker (Catostomus commersonii) yellow bullhead (Ameiurus natalis)

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