Facility management and equipment. Institut Pasteur du Maroc
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1 Facility management and equipment Institut Pasteur du Maroc
2 Laboratory biosafety is the term used to describe the containment principles, technologies and practices that are implemented to prevent unintentional exposure to pathogens and toxins or their accidental release. [WHO, Laboratory Biosafety Manual, 2004] 2
3 Containment objectives! BSL1 No defined containment objective (but: clean and hygienic work, avoid aerosols)! BSL 2 Minimize release! BSL 3 Prevent release! BSL 4 Prevent release even in case of failure of primary containment 3
4 Possible escape from the containment Exhaust Air Entrance Laboratory (Secondary Ccontainment) Sewage Waste Building Walls Windows Supply lines 4
5 A typical Biosafety Level 1 laboratory Walls and floor covering must be resistant to cleaning agents and easy to clean. Marked off as area for genetic engineering work (notices on door) Wash basin with emergency eye shower equipment BSL 1 Laboratory 5
6 Marking I BSL 1 Laboratory 6
7 A typical Biosafety Level 2 laboratory Label Biohazard Access control Window pane Safety cabinet inside working area Autoclav within building Tap water without manual contact Collect contaminated waste and water No open drains in floor 7
8 Marking II S2 - Genlabor Zutritt nur mit Berechtigung BIO II 8
9 Biosafety Cabinet Class II, Requirements Protection design: Personnel, Product, cross-contamination Leak tightness of housing - Liquids - Gas tightness Air flow - direction - velocity 2-Filter BSC Retention at the opening Cleanability of (Work-) surfaces 3-Filter BSC 9
10 Safe Centrifugation Transparent screw cap with sealing gasket 10
11 A typical Biosafety Level 3 laboratory Shielded, sealable area, windows not to be opened Safety cabinet and autoclave within working area Filtration of exhaust air in laboratory area required Negative pressure inside airlock pi < pa Collect all waste water liquids Sterilization Emergency reservoir 11
12 Access to S3 laboratory Laboratories in which activities of protection level 3 take place must be separated from other areas by an airlock with two interlocking, self-closing doors with a viewing window. (GenTSV Annex III; TRBA 100, page 19). v v 12 12
13 Possible escape - Exhaust Air The way in which HEPA filters are removed and decontaminated must be specified in the risk assessment. These must be removed in such a way that a hazard to the maintenance personnel and other persons can be ruled out. 1.Bag-in-bag changing system 2. (GenTSV Annex III; TRBA 100, page 21 ) Demonstration of filter change with bag-in-bag-system
14 Possible escape - Wastewater Liquid waste leaves the S3 laboratory through the autoclave. Thermische Abwassersterilisation für Waschwässer (Waschwassersterilisator) Thermische Abwassersterilisation Quelle: abwassersterilisation.htm
15 Possible escape - Walls and ceilings The spaces within the protection level area and in the contaminated part of the airconditioning system, up to and including the first HEPA filter stage, must be sealable for the purpose of fumigation.
16 Requirements for BSL 4 laboaratory Pressure graduation pi < p1 < p2 < p3 Filtration of air (in and out!) by double membrane filters Collect all wastes and waste water sterilization Material lock with forced inactivation Normal and work clothes separated Separate building or separated by aisle; hermetically sealed; no window available; utility piping shielded by automatic valves or filters 16
1. The laboratory supervisor must enforce the institutional policies that control access to the laboratory.
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