BIOSTAT CultiBag RM Culturing Convenience
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1 BIOSTAT CultiBag RM Culturing Convenience Fisher Scientific
2 Culturing Convenience The BIOSTAT CultiBag RM is the new generation of disposable bioreactors utilizing rocking motion for mixing with low shear. The combination of a new generation of Sartorius Stedim Biotech engineered control capabilities launches the disposable bioreactor system into a new era of cultivation. The disposable cultivation chamber CultiBag RM is specially designed for shear sensitive cells and any singleuse applications. A comprehensive validation and extractables report is available for the bags and can also be designed for your process. Maintenance and overhead expenses are reduced, eliminating the need for: Sterilization in Place (SIP) Cleaning In Place (CIP) Reduction in WFI Reduction in user time effort Reduction in validation requirements The measurement and control capabilities supplied by Sartorius Stedim Biotech are second to none. Utilizing proven technology and expert engineering, we have developed our existing in-house systems to bring powerful control capabilities to the disposable market. The BIOSTAT RM Control Tower presents an easy-to-use touch screen control with integrated measurement and control hardware, pumps, temperature and gassing systems, for excellent process control. The application-driven, configured packages for basic, optical and perfusion provide everything needed to get started immediately. The BIOSTAT CultiBag RM is available in scalable working volumes from 0.1 L to 300 L. Just select the size that meets your needs today. Each basic, optical and perfusion package also includes our BioPAT MFCS/DA data logging software. A large variety of operation modes facilitates a batch-oriented bioprocessing. Technical Specifications Dimensions Weight (kg) W + H + D (mm) BIOSTAT RM Control Tower mm 60 kg Rocker 20/50 with Holder mm 27 kg Rocker 20/50 with Holder mm 32 kg BIOSTAT CultiBag RM mm 272 kg BIOSTAT CultiBag RM mm 340 kg Utilities Power supply 120 VAC and 230 VAC 208 VAC and 400 VAC
3 The BIOSTAT CultiBag RM is ideal for Cell culture, growth of animal, insect and plant cells Shear sensitive cells or anaerobic bacteria Transition from shake or tissue culture flasks Seed Cultivation for large scale Bioreactors Production scale up to 300 L For all your disposable applications
4 The BIOSTAT CultiBag RM packages are specially configured to meet your disposable needs Basic Packages Used for simple and easy cultivations. The rocker is used as a standalone unit with the heater blanket for temperature control and is equipped with an integrated air pump and flow controller for bag inflation and supply to cells. Alternatively the unit may be placed inside an incubator for temperature control and air supply to the cells. Additionally, a pressure relief valve and flow controller are included for attachment to your own gas mixing device. Rocking rate and rocking angle can be adjusted individually. Optical Packages Designed for batch and fed-batch processes. Process control is achieved through disposable optical chemical ph and DO sensors. Feedback control of both values is available for your process. Two integrated pumps are included. Perfusion Packages Designed for continuous cultivation. Two additional integrated pumps are supplied for feed harvest control by weight. Balances are included for excellent perfusion control. BioPAT MFCS/DA All packages are equipped with BioPAT MFCS/DA software for external data logging. All batch related data can be stored in an individual folder allowing batchoriented bioprocessing. Its plotting module together with the integrated export function to MS Excel is both user friendly and highly flexible.
5 Packages Disposable optical chemical ph and DO sensors assure total process control in your application An easy to use touch screen interface makes the operation of your BIOSTAT CultiBag RM system very convenient and keeps training to a minimum Basic Packages Standalone Unit Rocker only Integrated pump or external airation module for inflation of bag and supply of ambient air to the cells Pressure relief and flow controller for connection to an external gas supply device Rocking angle and rocking rate control Temperature control heater blanket included Rocker may alternatively be placed into a CO 2 incubator BioPAT MFCS/DA data logging software included Optical Packages Standalone Unit Rocker with Optical Control Tower Optical Control Tower Disposable optical chemical ph and DO sensors are included Feedback control of ph Feedback control of DO Intuitive touchscreen Trend display 2 integrated pumps Pressure control for overpressure safety Gassing by 4 + rotameters and 2 + mass flow controllers to overlay aeration BioPAT MFCS/DA data logging software included Perfusion Packages Standalone Unit Rocker with Perfusion Control Tower Perfusion Control Tower Balances for feed harvest flow control are included Disposable optical chemical ph and DO sensors are included Feedback control of ph Feedback control of DO Intuitive touchscreen Trend display 4 integrated pumps Pressure control for overpressure safety Gassing by 4 + rotameters and 2 + mass flow controllers to overlay aeration BioPAT MFCS/DA data logging software included Bag Designs Basic Bags* Air inlet air outlet filter Sample port Fill drain and spare ports Female luer, female MPC or male MPC connectors Ports with dip tubes Customized bags also available Optical Bags* Air inlet air outlet filter Sample port Fill drain and spare ports Female luer, female MPC or male MPC connectors Ports with dip tubes Optical chemical DO sensor Optical chemical ph sensor Perfusion Pro Bags* Air inlet air outlet filter Sample port Fill drain and spare ports Female luer, female MPC or male MPC connectors Ports with dip tubes Optical chemical DO sensor Optical chemical ph sensor Feed harvest ports Acid base port Internal fixed perfusion membrane The needleless Septum port in all standard CultiBags RM faciliates your sampling process and keeps it safe at all times. * Bags not included in packages
6 Hardware Technical Specifications Description Rockers and Controllers Rocker 20/50 Rocker 200 Rocker 600 Bag Sizes 2 L, 10 L, 20 L, 50 L 100 L, 200 L 600 L Power Supply 120 VAC 208 VAC* 230 VAC 400 VAC* Rocker Includes Air Pump Pressure Regulator Rocking Angle (degrees) Rocking Rate (rocks/min) Basic Package Rocker only n/a n/a Heater Plate Bag Holder Filter Heater Tinted Lid Control Tower BioPAT MFCS/DA Optical Package Rocker + Rocker + Rocker + Optical Control Tower Optical Control Tower Optical Control Tower Heater Plate Optical Control Tower Rotameter for air, O 2, N 2, CO 2 MFC for Total Flow MFC for CO 2 Temperature Control ( C) ambient 40 ambient 40 ambient 45 ph Control DO Control % % % Cascade Control on Controller Aeration Module on Controller Peristaltic pumps (integrated) BioPAT MFCS/DA Perfusion Package Rocker + Rocker + Perfusion Control Tower Perfusion Control Tower n/a In addition to Optical Package: Peristaltic pumps 2 2 per side Balances 2 2 per side For additional information and order codes please refer to our datasheets BIOSTAT CultiBag RM 20/50, BIOSTAT CultiBag RM 200 and BIOSTAT CultiBag RM 600. or value = included = not included * Rocker 200 only
7 Bag Technical Specifications Description Sizes Standard Bags CultiBag Basic 1 L 2 L 10 L 20 L 50 L 100 L 200 L CultiBag Optical 2 L 10 L 20 L 50 L 100 L 200 L 600 L CultiBag Perfusion Pro 2 L 10 L 20 L 50 L 100 L 200 L Total Volume (L) Min Working Volume (L) 0.1* 0.2* 1* 2* 5* 10* 20* 60* Max Working Volume (L) Fittings (ID + OD) Air Inlet Filter Air Outlet Filter Sample port Female Luer, C-Flex 1/8" + 1/4", dip tube Female Luer, C-Flex 1/8" + 1/4" Female Luer, Silicone 3/16" + 5/16" Female MPC, C-Flex 1/4" + 7/16", dip tube Male MPC, C-Flex 1/4" + 7/16" - 1 Male MPC, C-Flex 3/8"+5/8" 1 1 Male MPC, C-Flex 3/8" + 5/8", dip tube ph and DO optical chemical sensor Female MPC, Pharmed 3/8" + 5/8", C-Flex 3/8" + 5/8", dip tube 1 Female MPC, C-Flex, 3/8" + 5/8", dip tube 1 Fittings CultiBag RM Perfusion Pro only: Perfusion membrane 1.2 μm Perfusion membrane 5 μm Female Luer, Pharmed 1/16" +3/16", C-Flex 1/8" + 1/4", dip tube 1 1 Female Luer, Pharmed 1/16" + 3/16", C-Flex 1/8" + 1/4" 1 1 Female Luer, Pharmed 1/8" + 1/4", C-Flex 1/8" + 1/4", dip tube 1 1 Female Luer, Pharmed 1/8" + 1/4", C-Flex 1/8" + 1/4" 1 1 Female Luer, Pharmed 3/16" + 5/16", C-Flex 1/4" + 7/16", dip tube 1 1 Female Luer, Pharmed 3/16" + 5/16", C-Flex 1/4" + 7/16" 1 1 or number = included = not included = applies to all Bag types = applies to basic Bags only = applies to optical Bags only = applies to perfusion Bags only * Bags with sensors require a higher minimum working volume than indicated For additional information and order codes please refer to our datasheet CultiBag RM.
8 Specifications subject to change without notice. BN Ver
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