IR-4 Ornamental Horticulture Program Research Report Cover Sheet

20111214c.pdf IR-4 Ornamental Horticulture Program Research Report Cover Sheet Researcher(s) Buzz Uber Affiliation: 31130 Hilltop Dr. Trial: 2011 CA Vaelly Center CA 92082 PhoneNumber: 760-751-2573 Email: buzzuber@cs.com ProjectTitle: Isoxaben Crop Safety Protocol #: 11-004 PR# Research Target Crop/Plant Product EPA Reg. # Production Site Status 24289 Phytotoxicity Butterfly Bush Gallery 75DF 62719-145 Field Container C Phytotoxicity Buddleia davidii Isoxaben Dow AgroScie

Dec-9-2011 (11-IR4-GALLERY-PHY) Site Description Page 1 of 12 2011 Crop safety with over-the-top Applications of select Herbicide Materials PR # 24289 Trial 11-IR4-GALLERY-PH ID: Y Protocol ID: 11-004-GALLER Y Study Rebecca Sisco Director: Study Director: Rebecca Sisco Title: Regional Field Coordinator Title: Owner General Trial Information Discipline: H herbicide Trial Status: F one-year/fina l Initiation Date: May-8-2011 Planned Completion Date: Jul-29-2011 Trial Location City: Valley Center Latitude of LL Corner : 33.27692 N USA 49.376656 State/Prov.: CA Longitude of LL Corner : 117.042155 W -124.715843 Postal Code: 92082 Country: USA Objectives: Altitude of LL Corner, Unit: 1532.00 FT - 24.53833 - -66.968887 Determine phytotoxicity of Gallery 75 DF to ornamental horticultural plants - Buddleia ssp. Conclusions: Gallery 75DF applied as an over-the-top application to Buddleia species used in this trial was considered a safe application as no significant or consistent injury was recorded for the duration of the trial. There were however few plants which exhibited possible stunting however this symptom was very inconsistent and could not be validated as injury due to treatment. No evidence of chlorosis, necrosis was observed in any of the treatments. Study Director: Rebecca Sisco Title: Affiliation: Western Region IR-4 Program Address: 4218 Meyer hall Location: One Shields Ave Postal Code: 95616 Phone No.: 530 752-7634 Regional Field Coordinator E-mail: rsisco@ucdavis.edu Title: Owner Affiliation: Address: 31130 Hilltop Drive Location: Valley Center, CA Postal Code: 92082 E-mail: buzzuber@cs.co m Mobile 760 805-3255 No.: Personnel Crop 1: BUDSS Buddleia sp. Butterfly bush Variety: Black Knight BBCH Scale: BPER Planting Date: May-11-2011 Crop Description

Site and Design Plot Width, Unit: 1.5 FT Site Type: NURCON nursery - container Plot Length, Unit: 3 FT Experimental Unit: 1 PLOT plot Plot Area, Unit: 4.5 FT2 Replications: 4 Study Design: RACOBL Randomized Complete Block (RCB) Untreated Arrangement: INCLUDED single control randomized in each block

Dec-9-2011 (11-IR4-GALLERY-PHY) Site Description Page 2 of 12 A B Application Date: May-13-2011 Jun-27-2011 Time of Day: 1030 1700 Application Method: TOPICA TOPICA Application Timing: POSPRE POSPRE Application Placement: BROFOL BROFOL Applied By: Buzz Uber Buzz Uber Air Temperature, Unit: 69 F 86 F % Relative Humidity: 60 24 Dew Presence (Y/N): N no N no Soil Moisture: NORMAL NORMAL % Cloud Cover: 0 0 Application Description A B Appl. Equipment: CO2 bckpck CO2 bckpck Operating Pressure, Unit: 30 PSI 30 PSI Nozzle Type: flat fan flat fan Nozzle Size: 8002 8002 Spray Volume, Unit: 87 87 GAL/AC GAL/AC Mix Size, Unit: 1 Liters 1 Liters Spray ph: 7.2 7.1 Propellant: CO2 CO2 Tank Mix (Y/N): N no N no Application Equipment

Trial Comments MATERIALS / METHODS PLOT OVERVIEW Plots were arranged in a commercial production like setting. One gallon containers were placed on a fabric "weed mat" with plots on 24" centers. Containers within each plot were spaced on 12" centers. PLANT MATERIAL Plants were "Liners" and obtained from a local producer of woody ornamental liner stock. All plants were considered to be in a good state of health with an established root system. Plants were considered to be at a suitable stage for transplanting. All liners were pruned to 3.0" prior to transplanting. MEDIA The mix was considered a standard "nursery mix" for commonly grown containerized woody ornamentals. The mix was composed of 25% sand + 75% fir bark shavings. NUTRIENTS All nutrients were provided with a 9 mos CRF (controlled release fertilizer) which was incorporated at time of blending other soil additives. No additional nutrients were supplied during the trial period. IRRIGATION Two hours following the herbicide application.60 inches of water was applied via overhead impact sprinklers (Robert's Irrigation) on a head-to-head design to both remove granules from foliage and move herbicide into upper soil profile. A rain gauge was strategically placed in the plot area to capture irrigation water for measurement. Two daily short cycle irrigations (60 sec) were made using a low volume FAIN spray stake type emitter displacing water in a 180 degree spray pattern over the soil surface. APPLICATION Over-the-top spray applications were applied using a CO2 backpack sprayer with a single flat fan nozzle flat fan wand held at 18" above the crop. A "drift hood" was used during the application to prohibit contamination of adjacent plots. CULTURAL NOTES In order to minimize affects of competitive weeds in plots a hand weeding was done just prior to app B. EVALUATION METHODOLOGY Evaluation methodology consisted of close observation for chlorosis, necrosis of young developing growth, stunting, overall plant quality and any other abnormality when compared to the UTC treatment.

Dec-9-2011 (11-IR4-GALLERY-PHY) Assessment Data Summary Page 4 of 12 2011 Crop safety with over-the-top Applications of select Herbicide Materials - PRN # 24289 Trial 11-IR4-GALLERY-PH ID: Y Protocol ID: 11-004-GALLER Y Study Rebecca Sisco Director: Crop Scientific Name Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Crop Name Butterflybush Butterflybush Butterflybush Butterflybush Butterflybush Butterflybush Crop Variety Black Knight Black Knight Black Knight Black Knight Black Knight Black Knight Description 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead Part Rated PLABRO - PLABRO - PLABRO - PLABRO - PLABRO - PLABRO - Rating Date May-20-2011 May-28-2011 Jun-11-2011 Jul-5-2011 Jul-12-2011 Jul-25-2011 Rating Type PHYGEN PHYGEN PHYGEN PHYGEN PHYGEN PHYGEN Rating Unit 0-10 0-10 0-10 0-10 0-10 0-10 Sample Size, Unit 1 PLANT 1 PLANT 1 PLANT 1 PLANT 1 PLANT 1 PLANT Number of Subsamples 3 3 3 3 3 3 Trt-Eval Interval 7 DA-A 15 DA-A 29 DA-A 8 DA-B 15 DA-B 28 DA-B Trt Treatment Rate Appl No. Name Rate Unit Cod Plot 1 2 3 4 5 6 e 1 101 0.0 0.0 0.0 0.0 0.0 0.0 101 0.0 0.0 0.0 0.0 0.0 0.0 101 0.0 0.0 0.0 0.0 0.0 0.0 204 0.0 0.0 0.0 0.0 0.0 0.0 204 0.0 0.0 0.0 0.0 0.0 0.0 204 0.0 0.0 0.0 0.0 0.0 0.0 303 0.0 0.0 0.0 0.0 0.0 0.0 303 0.0 0.0 0.0 0.0 0.0 0.0 303 0.0 0.0 0.0 0.0 0.0 0.0 401 0.0 0.0 0.0 0.0 0.0 0.0 401 0.0 0.0 0.0 0.0 0.0 0.0 401 0.0 0.0 0.0 0.0 0.0 0.0 Mean = 0.0 0.0 0.0 0.0 0.0 0.0 2 GALLERY 0.66 lb/a AB 102 0.0 0.0 0.0 0.0 0.0 0.0 102 0.0 0.0 0.0 0.0 0.0 0.0 102 0.0 0.0 0.0 0.0 0.0 0.0 201 0.0 0.0 0.0 0.0 0.0 0.0 201 0.0 0.0 0.0 0.0 0.0 0.0 201 0.0 0.0 0.0 0.0 0.0 0.0 304 0.0 0.0 3.0 0.0 0.0 0.0 304 0.0 0.0 0.0 0.0 0.0 0.0 304 0.0 0.0 0.0 0.0 0.0 0.0 402 0.0 0.0 0.0 0.0 0.0 0.0 402 0.0 0.0 0.0 0.0 0.0 0.0 402 0.0 0.0 0.0 0.0 0.0 0.0 Mean = 0.0 0.0 0.3 0.0 0.0 0.0 3 GALLERY 1.32 lb/a AB 103 0.0 0.0 0.0 0.0 0.0 0.0 103 0.0 0.0 0.0 0.0 0.0 0.0 103 0.0 0.0 0.0 0.0 0.0 0.0 202 0.0 0.0 4.0 0.0 0.0 0.0 202 0.0 0.0 0.0 2.0 0.0 0.0 202 0.0 0.0 3.0 0.0 0.0 0.0 301 0.0 0.0 0.0 0.0 0.0 0.0 301 0.0 0.0 0.0 0.0 0.0 0.0 301 0.0 0.0 0.0 0.0 0.0 0.0 403 0.0 0.0 0.0 2.0 0.0 0.0 403 0.0 0.0 0.0 0.0 0.0 0.0 403 0.0 0.0 0.0 0.0 0.0 0.0 Mean = 0.0 0.0 0.6 0.3 0.0 0.0

Dec-9-2011 (11-IR4-GALLERY-PHY) Assessment Data Summary Page 5 of 12 Crop Scientific Name Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Crop Name Butterflybush Butterflybush Butterflybush Butterflybush Butterflybush Butterflybush Crop Variety Black Knight Black Knight Black Knight Black Knight Black Knight Black Knight Description 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead Part Rated PLABRO - PLABRO - PLABRO - PLABRO - PLABRO - PLABRO - Rating Date May-20-2011 May-28-2011 Jun-11-2011 Jul-5-2011 Jul-12-2011 Jul-25-2011 Rating Type PHYGEN PHYGEN PHYGEN PHYGEN PHYGEN PHYGEN Rating Unit 0-10 0-10 0-10 0-10 0-10 0-10 Sample Size, Unit 1 PLANT 1 PLANT 1 PLANT 1 PLANT 1 PLANT 1 PLANT Number of Subsamples 3 3 3 3 3 3 Trt-Eval Interval 7 DA-A 15 DA-A 29 DA-A 8 DA-B 15 DA-B 28 DA-B Trt Treatment Rate Appl No. Name Rate Unit Cod Plot 1 2 3 4 5 6 e 4 GALLERY 2.64 lb/a AB 104 0.0 0.0 0.0 2.0 0.0 0.0 104 0.0 0.0 2.0 0.0 0.0 0.0 104 0.0 0.0 0.0 0.0 0.0 0.0 203 0.0 0.0 0.0 0.0 0.0 0.0 203 0.0 0.0 0.0 0.0 0.0 0.0 203 0.0 0.0 0.0 2.0 0.0 0.0 302 0.0 0.0 2.0 0.0 0.0 0.0 302 0.0 0.0 0.0 2.0 0.0 0.0 302 0.0 0.0 0.0 0.0 0.0 0.0 404 0.0 0.0 0.0 0.0 0.0 0.0 404 0.0 0.0 0.0 0.0 0.0 0.0 404 0.0 0.0 0.0 2.0 0.0 0.0 Mean = 0.0 0.0 0.3 0.7 0.0 0.0

Dec-9-2011 (11-IR4-GALLERY-PHY) Assessment Data Summary Page 6 of 12 Crop Scientific Name Buddleia sp. Buddleia sp. Crop Name Butterflybush Butterflybush Crop Variety Black Knight Black Knight Description Part Rated TERMIN - TERMIN - Rating Date May-11-2011 Jul-29-2011 Rating Type HEIGHT HEIGHT Rating Unit 1 1 Sample Size, Unit PLANT PLANT Number of Subsamples 3 3 Trt-Eval Interval -2 DA-A 32 DA-B Trt Treatment Rate Appl No. Name Rate Unit Cod Plot 7 8 e 1 101 6.0 22.0 101 6.0 21.0 101 6.0 21.0 204 6.0 21.0 204 6.0 20.0 204 6.0 21.0 303 6.0 21.0 303 6.0 22.0 303 6.0 21.0 401 6.0 21.0 401 6.0 23.0 401 6.0 22.0 Mean = 6.0 21.3 2 GALLERY 0.66 lb/a AB 102 6.0 20.0 102 6.0 22.0 102 6.0 22.0 201 6.0 18.0 201 6.0 23.0 201 6.0 22.0 304 6.0 19.0 304 6.0 19.0 304 6.0 18.0 402 6.0 20.0 402 6.0 19.0 402 6.0 18.0 Mean = 6.0 20.0 3 GALLERY 1.32 lb/a AB 103 6.0 20.0 103 6.0 21.0 103 6.0 19.0 202 6.0 20.0 202 6.0 21.0 202 6.0 19.0 301 6.0 20.0 301 6.0 20.0 301 6.0 21.0 403 6.0 19.0 403 6.0 22.0 403 6.0 23.0 Mean = 6.0 20.4

Dec-9-2011 (11-IR4-GALLERY-PHY) Assessment Data Summary Page 7 of 12 Crop Scientific Name Buddleia sp. Buddleia sp. Crop Name Butterflybush Butterflybush Crop Variety Black Knight Black Knight Description Part Rated TERMIN - TERMIN - Rating Date May-11-2011 Jul-29-2011 Rating Type HEIGHT HEIGHT Rating Unit 1 1 Sample Size, Unit PLANT PLANT Number of Subsamples 3 3 Trt-Eval Interval -2 DA-A 32 DA-B Trt Treatment Rate Appl No. Name Rate Unit Cod Plot 7 8 e 4 GALLERY 2.64 lb/a AB 104 6.0 21.0 104 6.0 22.0 104 6.0 20.0 203 6.0 20.0 203 6.0 22.0 203 6.0 24.0 302 6.0 19.0 302 6.0 18.0 302 6.0 20.0 404 6.0 22.0 404 6.0 21.0 404 6.0 20.0 Mean = 6.0 20.8

Dec-9-2011 (11-IR4-GALLERY-PHY) Assessment Data Summary Page 8 of 12 2011 Crop safety with over-the-top Applications of select Herbicide Materials - PRN # 24289 Trial 11-IR4-GALLERY-PH ID: Y Protocol ID: 11-004-GALLER Y Study Rebecca Sisco Director: Part Rated PLABRO = plant - broadleaves TERMIN = terminal Rating Type PHYGEN = phytotoxicity - general / injury HEIGHT = height Rating Unit 0-10 = 0-10 index/scale PLANT = plant/plant biomass/shrub

Dec-9-2011 (11-IR4-GALLERY-PHY) AOV Means Table Page 9 of 12 2011 Crop safety with over-the-top Applications of select Herbicide Materials - PRN # 24289 Trial 11-IR4-GALLERY-PH ID: Y Protocol ID: 11-004-GALLER Y Study Rebecca Sisco Director: Crop Scientific Name Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Buddleia sp. Crop Name Butterflybush Butterflybush Butterflybush Butterflybush Butterflybush Butterflybush Crop Variety Black Knight Black Knight Black Knight Black Knight Black Knight Black Knight Description 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead 0=none 10=dead Part Rated PLABRO - PLABRO - PLABRO - PLABRO - PLABRO - PLABRO - Rating Date May-20-2011 May-28-2011 Jun-11-2011 Jul-5-2011 Jul-12-2011 Jul-25-2011 Rating Type PHYGEN PHYGEN PHYGEN PHYGEN PHYGEN PHYGEN Rating Unit 0-10 0-10 0-10 0-10 0-10 0-10 Sample Size, Unit 1 PLANT 1 PLANT 1 PLANT 1 PLANT 1 PLANT 1 PLANT Number of Subsamples 3 3 3 3 3 3 Trt-Eval Interval 7 DA-A 15 DA-A 29 DA-A 8 DA-B 15 DA-B 28 DA-B Trt Treatment Rate Appl No. Name Rate Unit Code 1 2 3 4 5 6 1 0.0 a 0.0 a 0.0 a 0.0 c 0.0 a 0.0 a 2 GALLERY 0.66 lb/a AB 0.0 a 0.0 a 0.3 a 0.0 c 0.0 a 0.0 a 3 GALLERY 1.32 lb/a AB 0.0 a 0.0 a 0.6 a 0.3 b 0.0 a 0.0 a 4 GALLERY 2.64 lb/a AB 0.0 a 0.0 a 0.3 a 0.7 a 0.0 a 0.0 a LSD (P=.10) 0.00 0.00 0.91 0.25 0.00 0.00 Standard Deviation 0.00 0.00 0.70 0.19 0.00 0.00 CV 0.0 0.0 241.69 76.98 0.0 0.0 Grand Mean 0.0 0.0 0.29 0.25 0.0 0.0 Replicate F 0.000 0.000 0.540 1.000 0.000 0.000 Replicate Prob(F) 1.0000 1.0000 0.6665 0.4363 1.0000 1.0000 Treatment F 0.000 0.000 0.466 11.000 0.000 0.000 Treatment Prob(F) 1.0000 1.0000 0.7132 0.0023 1.0000 1.0000 Means followed by same letter do not significantly differ (P=.10, LSD)

Dec-9-2011 (11-IR4-GALLERY-PHY) AOV Means Table Page 10 of 12 Crop Scientific Name Buddleia sp. Buddleia sp. Crop Name Butterflybush Butterflybush Crop Variety Black Knight Black Knight Description Part Rated TERMIN - TERMIN - Rating Date May-11-2011 Jul-29-2011 Rating Type HEIGHT HEIGHT Rating Unit 1 1 Sample Size, Unit PLANT PLANT Number of Subsamples 3 3 Trt-Eval Interval -2 DA-A 32 DA-B Trt Treatment Rate Appl No. Name Rate Unit Code 7 8 1 6.0 a 21.3 a 2 GALLERY 0.66 lb/a AB 6.0 a 20.0 b 3 GALLERY 1.32 lb/a AB 6.0 a 20.4 ab 4 GALLERY 2.64 lb/a AB 6.0 a 20.8 ab LSD (P=.10) 0.00 1.30 Standard Deviation 0.00 1.00 CV 0.0 4.86 Grand Mean 6.0 20.63 Replicate F 0.000 1.117 Replicate Prob(F) 1.0000 0.3923 Treatment F 0.000 1.265 Treatment Prob(F) 1.0000 0.3437 Means followed by same letter do not significantly differ (P=.10, LSD)

Dec-9-2011 (11-IR4-GALLERY-PHY) AOV Means Table Page 11 of 12 2011 Crop safety with over-the-top Applications of select Herbicide Materials - PRN # 24289 Trial 11-IR4-GALLERY-PH ID: Y Protocol ID: 11-004-GALLER Y Study Rebecca Sisco Director: Part Rated PLABRO = plant - broadleaves TERMIN = terminal Rating Type PHYGEN = phytotoxicity - general / injury HEIGHT = height Rating Unit 0-10 = 0-10 index/scale PLANT = plant/plant biomass/shrub

Dec-9-2011 (11-IR4-GALLERY-PHY) Trial Treatments Page 12 of 12 2011 Crop safety with over-the-top Applications of select Herbicide Materials - PRN # 24289 Trial 11-IR4-GALLERY-PH ID: Y Protocol ID: 11-004-GALLER Y Study Rebecca Sisco Director: Trt Treatment Form Form Form Rate Appl Spray Volume Mix Mix No. Name Conc Unit Type Rate Unit Code Volume Unit Size Unit 1 2 GALLERY 75 % DF 0.66 lb/a AB 87 GAL/AC 0.5 Liters 3 GALLERY 75 % DF 1.32 lb/a AB 87 GAL/AC 0.5 Liters 4 GALLERY 75 % DF 2.64 lb/a AB 87 GAL/AC 0.5 Liters Replications: 4, Untreated treatments: 1, Design: Randomized Complete Block (RCB), Treatment units: US standard, Treated 'Plot' size Width: 1.5 feet, Treated 'Plot' size Length: 3 feet, Application volume: 87 gal/ac, Mix size:.5 liters, Mix overage: 10%, Format definitions: G-All7.def, G-All7.frm Product quantities required for listed treatments and applications of trials included in this table: Amount* Unit Treatment Name Form Conc Form Type Lot Code 6.363 g GALLERY 75 DF * 'Per area' calculations based on spray volume= 87 gal/ac, mix size= 0.5 liters (mix size basis). * Adjusted for multiple applications in treatment list.