HATCHERY QUALITY ASSURANCE PROGRAM

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HATCHERY QUALITY ASSURANCE PROGRAM for MURRAY COD, GOLDEN PERCH and SILVER PERCH Stuart J. Rowland and Patrick Tully

Murray cod Maccullochella peelii peelii Golden perch Macquaria ambigua Silver perch Bidyanus bidyanus

Murray-Darling River System

Conservation status Silver perch Vulnerable International Union for Conservation of Nature and Natural Resources (IUCN) Australian Society for Fish Biology NSW Fisheries (Fisheries Scientific Committee) South Australia Protected Victoria Critically endangered Murray cod Vulnerable Commonwealth Environment Protection and Biodiversity Conservation Act 1999 Victoria

1971 1978 1982 1983 Development of hatchery techniques - research commenced IFRS - Victorian Fisheries - MC - techniques developed large-scale production MC, GP, SP - First Freshwater Aquaculture Workshop, Narrandera 1982/83 - commercial industry

Inland Fisheries Research Station - 1980 (now the Narrandera Fisheries Centre)

Hatchery techniques Broodfish - wild, farm dams, fish farms Induced spawning - MC, GP, SP Natural spawning - MC Larval rearing in earthen ponds Harvest fry/fingerlings Stocking and sale

Collecting broodfish from the wild

Injecting HCG to induce spawning

Golden perch Fertilised and unfertilised eggs Larva about to hatch Hatched larva

Larval rearing pond Dry winter and early spring Filled and fertilised 10-14 days before larvae stocked

Harvesting, counting, stocking fingerlings

Hatchery production NFC ~ 2 million MC, TC, GP, SP GAC ~ 500,000 SP Commercial NSW 2-4 million Qld, others 2-4 million Total 5-10 million

Stocking Recreational fisheries - stock enhancement Conservation Aquaculture - commercial grow-out Farm dams

NSW Fisheries Concerns QDPI, other states Native Fish Strategy - MDBC Translocation & Stocking Workshop Canberra, September 2002 World Wildlife Fund, MDBC

Major concerns Genetics Diseases Trash fish (non-target fish) Quality (size, condition, health, weaned, graded)

for evolution Genetics of MC, GP, SP Closely related species and sub-species in other drainages Populations in M-D differ genetically and biologically natural selection and adaptation Low levels of genetic variation in the wild in hatcheries Genetic diversity is raw material

Hatcheries Broodfish Few parents Hybrids Genetics minimum no. broodfish most from farm dams, other hatcheries siblings low variation inbreeding SP X Welch s grunter

Genetics - NEED TO: Maintain GENETIC INTEGRITY Maximise GENETIC VARIATION Minimise DOMESTICATION

Diseases Pathogens on translocated fish world-wide problem Introductions to wild and farms white spot, Chilodonella to Australian Reports of diseased native fish ex hatcheries ectoparasites EUS tail and fin rot

Translocation of trash fish Fish farms implicated - CARP Trash fish on farms ex water supply; eels, gudgeons, carp, Gambusia Trash fish in consignments up to 4 species; SP in GP; GP in SP Banded grunter NT and NQ; now S-E Qld and Clarence R. NSW in batches of SP ex Qld hatcheries

HQAP - Chapters Site Selection Design and Operation Water Quality Disease and Health Management Chemicals and Drugs Broodstock and Population Genetics Breeding Programs Translocation and Stocking

HQAP Describes key features of hatchery Essential Criteria Accreditation and auditing Production and sale of MC, GP, SP stock enhancement, conservation commercial grow-out

Earthen Ponds: Essential Criteria Drainable by gravity Separate inlet and outlet Screens Harvest sump Records Sufficient ponds for species and breeding programs

Hatchery Building: Essential Criteria Filtration of water Spawning tanks Quarantine tanks Incubation tanks Screens on all tanks Aeration Office Support building(s) and workshop

Diagnosis of Disease: Essential Criteria Laboratory Microscope closed room, bench, sink, lighting, water high power x40 to x400 References Data sheets Dissecting equipment scalpels, scissors, slides

Dispatch of Fish: Essential Criteria Quarantine all fish for 24 h+ after harvest Chemical treatment in quarantine 2.5 g/l NaCl; 10 g/l NaCl + 50 mg/l formalin Examine 5 fish, 24 h prior to dispatch parasites on gill and skin tissue abnormalities; moribund or emaciated fish removed Dispatch Statement

Broodstock: Essential Criteria At least 10 pairs MC and GP from each river/population to maintain integrity and variation Do not use fish from farm dams Records unknown origin, genetics, parentage, age Tag all broodstock Hold fish from each river separately separate pond, cage, tank Replace GP and SP after 4-5 years

Breeding Program

HQAP Guidelines for Best Practice Basis for Accreditation and Auditing Benefits CONSERVATION RECREATIONAL FISHERIES POPULATIONS of NATIVE FISH COMMERCIAL AQUACULTURE

HQAP status Fish Stocking Fisheries Management Strategy (FMS) Hatcheries contacted/visited 2006 Hatchery Accreditation Scheme accreditation auditing in place 2008

BENEFITS of the HQAP