1 RFQ Form Bioengineering RALF Advanced Autoclavable Laboratory Bioreactors / Fermenters Inquirer Info Name Company Position Address City, Zip code Country E-mail Phone Local representative RFQ form ref. no. For each differently configured RALF unit within a request, a separate RFQ form has to be filled in and named with a number, starting with 1. When configuring RALF Advanced Multi Systems, RFQ form numbers can be used for reference in order to define which units shall be combined to a Multi System. Module Options Models + Volumes Application RALF Advanced microbial RALF Advanced cell culture Placement and mode of operation Individual Bioengineering RALF * Multi Bioengineering RALF system **, together with RFQ form numbers: Volumes + units 2.0 L: units 3.7 L: units 5.0 L: units 6.7 L: units If configuration of units shall be different, for each different unit an extra RFQ form has to be filled in. * Individual Bioengineering RALF: each Bioengineering RALF requires its own PC and may be placed and operated completely independently. ** Multi Bioengineering RALF system (up to 6 units): the reactors of a Multi Bioengineering RALF system are operated from one PC. Vessel Microbial Cell culture Vessel Double wall vessel (instead of single wall) Single wall vessel (instead of double wall) Baffles Baffles* Baffles Magnetic drive Magnetic drive (instead of direct drive) Impeller Pitched blade impeller (replaces marine / Rushton) Temperature Control Perfused baffles Double jacket * Baffles only with double wall vessel. Heating circuit connected to perfused baffles (with single wall vessel) Heating circuit connected to double jacket (with double wall vessel)
2 Aeration Microbial Cell culture Sparger Sinter sparger (replaces ring sparger) Aeration tube (replaces ring sparger) Ring sparger (replaces sinter sparger) Optional: change gas module I ** 1 x MFC 3 x pulsed 1 x MFC + 2 x pulsed 1 x MFC + 2 x pulsed 2 x MFC + 1 x pulsed 2 x MFC + 1 x pulsed Gas module I: alternative flow rates (optional) 1-gas modules: 5 8 16 5 / 5 / 2 8 / 8 / 5 16 / 16 / 8 40 / 40 / 8 40 100 250 500 L / h 100 /100 /16 L /h 3-gas modules: 5 / 5 / 2 8 / 8 / 5 16 / 16 / 8 40 / 40 / 8 100 / 100 / 16 L / h Gas module II (optional) *** 1 x MFC 1 x pulsed (1 vvm O2) 1 x MFC 1 x pulsed (0.1 vvm N2) 3 x pulsed 1 x MFC + 2 x pulsed 2 x MFC + 1 x pulsed (0.1 vvm Air, 0.1 vvm O2, 0.05 vvm CO2) Gas module ll: alternative gases and flow rates (optional) 1-gas modules: 5 8 16 40 100 250 500 L / h Air N2 O2 3-gas modules: 5 / 5 / 2 8 / 8 / 5 16 / 16 / 8 40 / 40 / 8 100 / 100 / 16 L / h Surface aeration N / A Surface aeration module (only in combination with a gas module II) Microbial Cell cultur ** If no selection, Bioengineering RALF Advanced microbial will come with 1 channel for 2vvm Air with pulsed valve and Bioengineering RALF Advanced cell culture with 3-channel gas mixing station for 0.1vvm Air, 0.1vvm O2 and 0.05 vvm CO2 with pulsed valves. Max. 2 MFCs per fermenter unit. First MFC in gas module will be Air, second MFC will be O2. Third gas in a module is always CO2. *** Max. 2 MFCs per fermenter unit. First MFC in a 3-cahnnel gas module will be Air, second MFC will be O2. Third gas in a module is always CO2. e Addition / Transfer Microbial Cell culture Optional height-adjustable immersion tube [in addition to regular immersion tube] Rotor filter package 20 μm N / A Rotor filter package 20 μm Secondary pump module 2 f Additional pump module with 2 fixed speed pumps Secondary pump module 2 f + 1v Additional pump module with 2 fixed speed and 1 variable speed pumps Additional side pump Additional external pump with display Laptop Laptop with preinstalled BioSCADA Power supply 230 V, 50 60 Hz 110 V, 50 60 Hz Additional Options Vessel Rushton impeller 40 mm 48 mm 60 mm diameter [3.7 L and larger only] Marine impeller 48 mm 66 mm diameter [3.7 L and larger only] Pitched blade impeller 48 mm 66 mm [3.7 L and larger only] Temperature Control Chiller Chiller, 50 L, for up to 6 Bioengineering RALF 110 V, 60 Hz 230 V, 50 Hz Water supply connection set Water supply connection set for up to 4 Bioengineering RALF units Aeration Aeration tube Aeration tube 275 mm [2 5 L] 375 mm [6.7 L] Ring sparger Ring sparger 300 mm, narrow [2 L] 300 mm, wide [3.7 5 L] 400 mm, wide [6.7 L] Sinter sparger Sinter sparger 2 L 3.7 L 5 L 6.7 L Surface aeration module Surface aeration module (for existing 2nd gas module)
3 Addition / Transfer Bottle, 250 ml, incl. cap + filter Glass bottle, 250 ml, with cap + filter Bottle holder Glass bottle mounting brackets Screw cap for bottle Autoclavable screw cap Connection for lid Connection piece 4 mm for lid, 10 mm length Connection piece for hoses Connection piece with cup for needle - hose connection Cup with diaphragm Cup DN12 with diaphragm for lid inoculation Immersion tube Immersion tube 245 mm [2 5 L] 345 mm [6.7 L] Height-adjustable immersion tube Hypodermic needle 2 mm 3.5 mm 4 mm [inside diameter] Rotor filter Rotor Filter 127 mm [2 5 L] 222 mm [6.7 L] 10 μm 20 μm 30 μm 40 μm 75 μm sieve Sampling system Sampling system with mounting brackets Exhalyzer Exhaust gas analyzer O2 and CO2 concentration 110 V, 60 Hz 230 V, 50 Hz Redox probe with transmitter Redox probe, with transmitter For 2 5 L vessel For 6.7 L vessel Balance 10 35,000 g Balance 10 35,000 g Balance 1 16,100 g Balance 1 16,100 g Balance 1 6,500 g Balance 1 6,500 g Optical density Optical density probe with controller For 2 5 L vessel For 6.7 L vessel Vessel Kits - Minimal Package For every RALF system additional autoclavable vessels are available. Such additional vessel kits allow change of vessel volume or also storage of pre-sterilized vessel kits in order to avoid dead time between two cultivations. The minimal vessel kit packages can be completed and individually equipped by the vessel kit options listed further down. Vessel Microbial Cell culture Volume 2.0 L 3.7 L 5.0 L 6.7 L Vessel Double wall vessel Single wall vessel (without baffles) (with perfused baffles for heating + cooling) Spare vessel kit, autoclavable, minimal package. Including: glass vessel with lid and blind plugs, bearing and shaft, sampling / harvest tube, Pt100, condenser with hoses and connection pieces, inlet and outlet gas filter with hoses, check valve. Options for Vessel Kit Vessel Baffles * Baffles * Rushton impeller Rushton impeller Marine impeller Marine impeller Pitched blade impeller Pitched blade impeller * Baffles only with double wall vessel. Aeration Aeration tube Ring sparger Sinter sparger Aeration tube Ring sparger Sinter sparger
4 Addition / Transfer Height-adjustable immersion tube Rotor filter with immersion tube Rotor filter, 20 μm sieve, with height-adjustable immersion tube 2 x bottle 2 x 250 ml bottle, with cap, incl. mounting brackets 3 x bottle 3 x 250 ml bottle, with cap, incl. mounting brackets Sampling system Autoclavable sampling system ph probe po2 probe Antifoam / level probe Autoclavable ph probe Autoclavable po2 probe Antifoam / level probe Spare Parts Set of spare parts, small For 2 L For 3.7 L For 5 L + 6.7 L vessel Set of spare parts, large For 2 L For 3.7 L For 5 L + 6.7 L vessel Spare filter package Set of spare filters for gas in- and outlet
5 Service Shipping Shipping EXW Wald, Switzerland CIP airport Door-to-door (CH, EU, USA) Destination City, Zip code Country Your Questions or Comments Please e-mail this form to your local representative or to: Bioengineering AG - Sagenrainstrasse 7-8636 Wald - Switzerland Phone +41 55 256 8111 Fax +41 55 256 8256 info@mybioreactor.com