Sampling Equipment Lab FISH 415 LIMNOLOGY UI Moscow Purpose: - to familiarize you with limnological sampling equipment - to use some of the equipment to obtain profiles of temperature, dissolved oxygen, conductivity and light. - to produce limnological figures of the profiles. PART I EQUIPMENT Attached are drawings and pictures of standard limnological sampling equipment. You will be responsible for knowing the name and use of each. Be sure you understand the proper use of each as well as the operation of trigger mechanisms. If not sure ask. PART II SAMPLING We will use multi-probe meters to obtain data from two artificial lakes outside the CNR building. Record all of your observations and make sure a copy is turned in to the T.A. for posting. Your data will be available via and excel spreadsheet on the course web page after the lab. 1) Use the YSI DO/temp meters and probes to obtain profiles of temperature ( C), dissolved oxygen (mg/l and % saturation), conductivity (ìs) and ph of each of the two lakes. Use 0.1-0.3 m intervals - the smaller the interval, the better your profile resolution. Typically the interval would be 0.5 to 1 m. ASSIGNMENT 1 Analysis of Profiles Purpose: - to analyze collected data and produce limnological figures - learn use of spreadsheets - perform simple statistics, regression analysis to calculate extinction coefficients. Data you will use are those you collected from the limnology lakes as well as some recent ones collected from a reservoir in Oregon. This will give you a chance to compare different water bodies. The data are available from the course website (http://www.cnr.uidaho.edu/fish415/) Labs and field trip tab. Computer spreadsheet You should be able to complete this lab on any computer running Microsoft Excel. (Note these instructions assume you are running Microsoft 2007 - the most recent install on all university computers - if you are using an older version, the menus will be slightly different. Given the university software agreement with Microsoft you can obtain 2007 at no charge, use it. Be sure you are comfortable with how to maneuver around in the spreadsheet, graph the
data, combine plots on one page, and the proper anatomy of a scientific figure. You will be expected to hand in your figures in proper format. There are computer labs available for your use across campus - check the IT website for info and hours. Tasks: 1) Plot profiles of temperature, dissolved oxygen (mg/l only), conductivity, ph and light for the Limnology Lakes and Willow Creek Reservoir. Place the data for the two limnology lakes on separate graphs on one page (i.e., one plot for all temperature profiles, one for all oxygen etc. and those for Willow Creek on a separate page (5 graphs/page = 2 pages). Each profile on each graph should be easy to distinguish (use different symbols and line types - do not use color). Make sure each figure has a proper caption that indicates what is the figure is about. (The idea here is that if the figure fell out of your notebook and someone picked it up they could interpret it). Write a short paragraph to summarize major trends in the data for each figure. This should be similar to the results section of a published manuscript - check the results section of the primary literature (journals e.g., L&O) for examples. 2) For light profiles for Willow Creek Reservoir, calculate the extinction coefficient. Do the calculations in two ways: i) calculate the extinction coefficient for each interval for each profile and then calculate the average for each profile; and ii) perform a regression analysis using depth as the independent variable and ln (natural log) irradiance as the dependent variable. The slope of this relationship will be the extinction coefficient (ignore the negative). (For regression, you will need to have the MS Analysis Tool pack installed on your computer - Excel typically does not install this automatically during a normal install. Go to Tools and see if you have a Data Analysis option - if not, go to Add Ins and check Analysis ToolPack - Excel may require the original installation disk). Use the table on sheet 2 of the spreadsheet - it is already set up for you. Don t forget that you want to use ln (natural log) of irradiance as the dependent (Y) variable. Prepare a proper table listing your calculated extinction coefficient(s). Calculate the depth at which 1% of the surface irradiance remains for each profile. How does this compare to 2z? sd Figure 1A. Plot of temperature versus depth for Campus Lake on August 22, 2003, Jackson Co. Illinois, USA. Note: I expect figures to be in the proper limnological format, and properly labeled. Check a
common journal to ensure you have all the necessary information on each figure. Your narratives should be grammatically correct and free of spelling errors. You don t have to put the figure caption below each figure, instead you can hand in a page of figure captions listing the figure number and corresponding caption. Grading Criteria for assignment Figures (Each) 5 - figures complete including caption (labeled axes, units, lines and symbols different for different sites or times, all details present, no color). For profiles, Depth on y axis, increasing from top to bottom, variable measured on x axis at top of graph. 4 - figure caption missing or incomplete, use of color 3 - figure caption missing or incomplete, symbols and lines do not clearly distinguish sites or times. 2 - figure caption missing or incomplete, symbols and lines do not clearly distinguish sites or times, axes not labeled or units missing. 1 - figure caption missing or incomplete, symbols and lines do not clearly distinguish sites or times, axes not labeled or units missing, variables on wrong axes. Narrative (Each) 3 - complete paragraph, including topic sentence, highlights major trends seen in accompanying figure in writing. Includes range of numbers allowing reader to quantitatively reconstruct figure. 2 - paragraph structure incomplete, missing highlight of major trends or fails to include overview of main numbers from figure. 1 - paragraph not complete, does not include major trends in accompanying figure and does not include main numbers from figure. Extinction 5 - correctly calculated for all 3 profiles, table completed coefficient 4 - Not all extinction coefficients correct, table completed 3 - incorrect coefficients, tables not completed 2 - partial execution of calculations 1 - no extinction coefficients completed