Angela Nikolic. List of Legislation, Code of Practice, Australian Standards, Guidance Materials used to determine control measures

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Use this form to assist you to complete risk assessments for hazardous activities and processes. Any serious or ongoing hazards should be reported via RiskWare to ensure that appropriate corrective actions are tracked and completed. Faculty/School: Faculty of Science / School of Molecular Bioscience Risk Assessment Reference Number: SMB007.2 Initial Issue Date: Oct 2015 Next Review Date: Oct 2016 Risk Assessment Name: Centrifugation: High Speed Floor Centrifuges Prepared by: Angela Nikolic Responsible supervisor/s: Markus Hofer (Chair WHS committee) & Dianne Fisher (Safety Officer) Identify the activity and the location Activity or process: Identify who may be at risk This may include fellow workers, students, visitors, contractors and the public Persons at risk: Staff and students at SMB that use these s. Location: Undergraduate and Research laboratories Risk assessment team (Who was consulted?): WHS committee at SMB List of Legislation, Code of Practice, Australian Standards, Guidance Materials used to determine control measures refer to Safe Work Procedure SWP007.2 Risk Assessment Methodology Assessing the risk is a brainstorming exercise, which is most effectively carried out in a team environment with the people required to complete the activity or process. Most activities or processes are broken down into a variety of separate tasks. For each task, consider the hazards, the potential harm or negative outcomes and the conditions required for those negative outcomes to occur. Whenever assessing the health and safety risks associated with a task, always consider the following primary risk factors. The physical activities required to complete the task e.g. repetitive movement, high force, physical exertion, awkward posture The work environment e.g. lighting, work layout, traffic, thermal comfort, working in isolation The nature of the hazard itself e.g. working with chemicals, microorganisms, radiation, machinery, potentially violent clients The individual workers involved, e.g. level of training, skills, experience, health, age, physical capacity The information gathered from the risk assessment process must be used to develop a Safe Work Procedure (SWP).

Task or scenario Hazard/s Associated harm, e.g. what could go wrong? Existing Risk Controls Current risk rating Use the Risk Matrix Any additional controls are required? 1 Residual risk rating Use the Risk Matrix Rotor imbalance. (explosion). injured. Always balance the tubes (by weight, not just by eye), to 0.1 g and put the balanced tubes in opposing positions in the rotor. Rotor can fail due to excessive speed. injured. Know the speed limits of the rotor and don t exceed these. Max speed varies from one rotor to another; large rotors cannot be spun as fast as small ones. Rotor can disengage from spindle injured Ensure that the rotor is firmly attached to the spindle and that the rotor lid is VERY TIGHT before starting the machine. This is done by screwing both rotor lid screws down tightly. Rotor can fail due to extensive wear and tear injured Inspect rotors yearly by certified technicians, replace if damaged. Newer rotors generally have expiry or use-by dates so please take note of these dates, contact supplier if date has been reached. 1 Always consider whether or not it is possible to eliminated the hazard or hazardous task altogether. If this is not possible, refer to the hierarchy of risk controls.

Centrifuge tubes can break in rotor due to excessive wear and tear or inappropriate use, causing imbalance of rotor. injured Ensure you use the correct tubes for the sample type, and do not use cracked or broken tubes. Phenol and chloroform will rapidly destroy polycarbonate tubes and must be used in polypropylene tubes. You should also only use tubes suited to the rotor. Please check with custodian of for which tubes are to be used. Autoclaving of tubes also shortens their lifespan. Not waiting for to reach speed reached before walking away If anything is going to go wrong (e.g. an imbalance), it will usually occur in this initial period. You need to be there to take action if this occurs. Page 3

Implementation of Additional Risk Controls Additional controls needed Resources required Responsible person Date of implementation RiskWare Reference Safe Work Procedure (SWP) DONE WHS committee 3.6.14 N/A Train workers to complete process in accordance with SWP Time supervisor and workers Supervisor N/A University and local WHS training Time Supervisor N/A List emergency controls for how to deal with fires, spills or exposure to hazardous substances and/or emergency shutdown procedures Follow general emergency and spill procedures. REVIEW Scheduled review date 1 year 2 years 3 years Are control measures in place (YES/NO) Are controls eliminating or minimizing the risk (YES/NO) Are there any new problems with the risk (YES/NO) Reviewed by: Actual Review date: yes minimising no

Risk Matrix. RISK ASSESSMENT FORM

In signing this section the assessor agrees that the following persons are competent in following this Risk Assessment. Name Signature Date Competent Name Assessor/Authoriser of Assessor/Authoriser signature Page 6