THE 1998 INTERNATIONAL COOPERATIVE SALMON RESEARCH CRUISE OF THE OSHORO MARU

NPAFC Doc. 349 THE 1998 INTERNATIONAL COOPERATIVE SALMON RESEARCH CRUISE OF THE OSHORO MARU by Robert V. Walker and Kerim Y. Aydin University of Washington FISHERIES RESEARCH INSTITUTE Box 357980 Seattle, Washington, USA 98 195-7980 Gen Anma, Hidekazu Yamaguchi, and Yoshihiko Kamel Hokkaido University FACULTY OF FISHERIES Hakodate, Hokkaido, Japan 041 Takayuki Shoji Hokkaido University FACULTY OF PHARMACEUTICAL SCIENCE Sapporo, Japan O60-0812 Masahide Kaeriyama Hokkaido Tokai University DEPARTMENT OF MARINE SCIENCES AND ThCHNOLOGY Sapporo, Japan 005-860 1 Shigehiko Urawa Fisheries Agency of Japan NATIONAL SALMON RESOURCE CENThR Sapporo, Japan 062-0922 submitted to the NORTH PACIFIC ANADROMOUS FISH COMMISSION by JAPAN AND THE UNITED STATES OF AMERICA September 1998 THIS PAPER MAY BE CITED IN THE FOLLOWING MANNER: Walker, R.V., K.Y. Aydin, G. Anma, H. Yamaguchi, Y. Kamei, T. Shoji, M. Kaeriyama, and S. Urawa. 1998. The 1998 international cooperative salmon research cruise of the Oshoro maru. (NPAFC Doc. 349.) FRI-UW-98 12. University of Washington, Fisheries Research Institute, Box 357980, Seattle, WA 98 195-7980. 20 p. Rev.

THE 1998 INTERNATIONAL COOPERATIVE SALMON RESEARCH CRUISE OF THE OSHORO MARU ABSTRACT Preliminary information is presented on international cooperative salmon research conducted during the June-July 1998 cruise of the OshorO maru. An objective of cooperative high-seas salmon research conducted under the North Pacific Anadromous Fish Commission Science Plan is salmon stock assessment through annual surveys along standard transects in the North Pacific Ocean and Bering Sea. Salmon surveys conducted aboard the Oshoro maru along 1800 longitude in the central North Pacific Ocean in June since 1978 have provided a valuable time series of fisheries and oceanographic data. This was the fifth consecutive year of cooperative Japan-U.S. sampling for salmon along a 145 W-longitude transect in the central Gulf of Alaska in early July, and the first year of a new transect along 165 W. The primary Obj~ètive of the 1998 cooperative research was to continue the collection of oceanographic and biological data along the 180, 165 W, and 145 W transects. In 1998, mid-june sea surface temperatures (SSTs) at gillnet fishing stations were about the same as in 1997 at 43 and 45 N and 1.3 C cooler (5.9 C) at 47 N along the 180 transect. Late-June mean SSTs were about 8.4 C at four gillnet stations on the 165 W transect, and early July mean SSTs (10.2 C) were 2.2 C cooler along the 145 W transect than in 1997. This represents a return to cooler conditions found from 1991 to 1996 at 145 W (mean 9.8 C). Catches by gillnet totaled 3,165 salmonids, including 256 salmonids (225 in 1997) in the central North Pacific Ocean (180 transect), 645 salmonids along the 1 65 W transect, and 2,264 salmonids (1,811 in 1997) in the Gulf of Alaska. At longline stations, 10 salmon (11 in 1997) in the central North Pacific Ocean, 41 salmon along 165 W, and 28 salmon (28 salmon in 1997) in the Gulf of Alaska were tagged and released. Temperature-recording data tags were attached to 13 of the tagged salmon, one at 48 30 N, 165 W and 12 along 145 W. Three doubletagged salmonids carrying temperature data tags and released in the Gulf of Alaska were recovered in Alaska. A pink salmon tagged on 3 July was recovered in a commercial purse seine fishery off Afognak Island (near Kodiak Island, Alaska) on 24 July 1998. A steelhead trout released on 9 July was recovered in a commercial gillnet fishery at the delta of the Copper River, Alaska on 14 August 1998. A coho salmon tagged on 3 July was recovered in a commercial gillnet fishery in Togiak Bay, Alaska on 24 August 1998. Biological samples and data were collected for various other cooperative studies of salmon distribution, abundance, stock origins, maturity and growth, food habits, bioenergetics, and other aspects of ocean biology and ecology; results will be reported later. INTRODUCTION This document reports on preliminary results of the 1998 international cooperative salmon (Oncorhynchus spp.) research cruise of the T/S Oshoro maru in the central North Pacific Ocean and Gulf of Alaska. An objective of international cooperative high-seas

2 salmon research conducted under the North Pacific Anadromous Fish Commission (NPAFC) Science Plan is salmon stock assessment through annual surveys along standard transects in the North Pacific Ocean and Bering Sea (FAJ 1994a,b; NPAFC 1995, 1996, 1997). Salmon surveys conducted by the Oshoro maru, Hokkaido University, Faculty of Fisheries, along 180 in the central North Pacific Ocean since 1978 have provided a valuable time series of fisheries and oceanographic data. In recent years, the Faculty of Fisheries and the Fisheries Research Institute (FRI), School of Fisheries, University of Washington, have cooperated in salmon research aboard the Oshoro maru in the central North Pacific and Gulf of Alaska (Walker 1993, Walker and Myers 1994, Walker et al. 1994, Myers et al. 1995, 1996, 1997). In 1998, scientists from the Fisheries Agency of Japan (FAJ), Hokkaido Tokai University, and the Canadian Department of Fisheries and Oceans also participated in the Gulf of Alaska survey. The primary objective of the 1998 cooperative salmon research was to continue the collection of oceanographic and biological data along the 180 transect in the central North Pacific and the 145 W transect in the central Gulf of Alaska and along a new transect at 165 W. METHODS Survey Area and Cruise Schedule Hydrographic, plankton, salmonid sampling, and additional sampling for salmonid feeding ecology, growth, maturity, and stock identification studies was conducted in the central North Pacific Ocean and Gulf of Alaska (Fig. 1, Tables 1 and 2). In international waters, surface longlines (B-gear) and gillnets (C-gear is non-selective varied research mesh and A-gear is commercial mesh) were used to catch salmonids. At stations within the U.S. 200-mile zone, only the surface longline was used to catch fish. Along 180 longitude in the central North Pacific Ocean, fishing for salmonids was conducted northward from 39 N to 50 N latitude. Along the 165 W transect, fishing occurred at stations southward from 52 40 N to 45 30 N. Along the 145 W transect, salmonids were sampled southward from 56 N to 50 N. The Oshoro maru departed Hakodate on 3 June 1998. From 9 to 17 June, salmon and oceanographic research was conducted northward along the 180 transect. The vessel made a port call in Dutch Harbor, Alaska, from 20 to 22 June, where scientists participating in the Gulf of Alaska cruise leg boarded. From 22-29 June, oceanographic and salmon research was conducted along 165 W longitude. From 2-10 July salmon and oceanographic research was conducted along 145 W in the Gulf of Alaska. On 13 July, the Oshoro maru arrived in Seattle, Washington, where Gulf of Alaska participants disembarked. On 17 July, scientists participated in a post-cruise workshop, Ecosystems of the North Pacific. Oceanographic Sampling Oceanographic research conducted in 1998 included hydrographic, plankton, larval fish, and beam trawl sampling (Table 1A-D). Computer files of CTD data summaries were used to plot temperature and salinity isopleths along the 180, 165 W, and 145 W transects.

3 Gillnet Sampling Gilinet sampling was conducted by Oshoro maru personnel (Table IE). Gilinet gear was set in the evening, allowed to soak overnight, and was retrieved the following morning. As the gilinet was hauled, the catch was sorted into baskets by mesh size and species. As the sorted fish were moved down the processing line, scale samples were collected, and species, fork length (mm), body weight (g), sex, and gonad weight (g) were recorded by mesh size on biological data forms. The catch by mesh size and species was recorded in an operations book, and was later entered into a computer file. Longline Research and Tagging All viable salmonids ãaught on longlines were double-tagged with both FAJ (red and white, 1.6 cm in diameter) and FRI (red and white, 2.0 cm in diameter) Petersen disk tags (Table 1E). Some salmonids were also tagged with thermal logger tags, archival tags which record temperature (Walker et al. 1998). Significant efforts were made by Oshoro marit personnel to minimize handling and holding time of salmon prior to release. As the longline was retrieved, the fish were landed in a dipnet and quickly put into a tank with flowing water for recovery. If possible without injuring the fish, the longline hook was removed. Viable fish were removed from the tank and placed on a measuring board. Fork length (mm) was measured, and a scale sample was taken. In regular tagging with Petersen disks only, the tags, which have a hole in the center, were threaded onto a plastic cinch strap, which is inserted into a hollow needle. The fish was held firmly upright, and the needle was inserted through the dorsal musculature, just in front of the dorsal fin. The plastic strap was quickly cinched, and the tagged fish was either put in the holding tank to recover or released immediately over the side. Data on species, length, and tag number of each fish were recorded on data forms. Temperature-recording archival data tags (40 x 23 x 8 mm; 9.5 g) were attached with two 76 mm pins inserted through the back just anterior to the dorsal fin. On the opposite side of the fish, the pins were inserted through FAJ and FRI disk tags; the ends of the pins were then twisted with pliers into knots lying flush with the disk tags. During the tagging procedure, the fish were held in a V-shaped tagging cradle which minimized movement. The cradle was immersed in a shallow trough with running seawater to minimize hypoxic damage to the fish during the procedure, which took slightly longer than standard tagging with a single cinch strap. Collection of scales and data on length, species, and tag numbers was the same as in the standard method. Only fish with good likelihood of recapture (based on maturity, condition at capture, and likely fisheries encounter) within a few months were tagged. This criterion meant that only maturing sockeye (0. nerka) and steelhead (0. mykiss) were evaluated for tagging along the 165 W transect, and maturing sockeye, chum (0. keta), pink (0. gorbuscha), coho (0. kisutch), chinook (0. tshawytscha)and steelhead were evaluated along 145 W. Fish Lacking Adipose Fins By prior arrangement with FAJ, snouts were collected from salmonids lacking an adipose fin (Table 2C). Snouts collected from fish lacking adipose fins were labeled with catch and biological information and frozen. After the Seattle port call, snout samples

4 were shipped to the U.S. National Marine Fisheries Service, Auke Bay Laboratory (ABL), where they were examined for coded-wire tags. FRI reports release and recovery information on coded-wire tags to NPAFC. Scale Sampling Scale samples were collected for verification of species identification, and for age, growth, and stock origin studies (Tables 1 and 2). Scale samples were collected by Oshoro nzaru personnel from all longline-caught fish and from up to 30 fish of each species caught in each mesh size of gillnet used in each set. All scales were collected from the International North Pacific Fisheries Commission (INPFC) preferred body area (identified by the letter 1A on data forms; Davis et al. 1990), except in cases where all preferred scales were missing (identified by the letter C on data forms), and placed on gummed cards. Additional Biological Sampling At gillnet and longline stations in the Gulf of Alaska, additional research activities by Canadian, Japanese, and U.S. scientists included collection of salmonid stomachs, blood, brains, olfactory organs, otoliths, muscle, liver, heart, eye, and scales for food habits, growth, maturity, migration, and stock identification studies (Table 2). RESULTS AND DISCUSSION Oceanographic Conditions Temperature and salinity isopleths along the 180, 165 W, and 145 W transects in 1998 are shown in Figure 2. In 1998, mid-june sea surface temperatures (SSTs) at the gilinet fishing stations at 43 N (8.8 C) and 45 N (7.8 C) were about the same as in 1997 and were 1.3 C cooler at 47 N (5.9 C) along the 180 transect. Late-June mean SSTs were 8.4 C along 165 W. Early-July mean SSTs (10.2 C) were 2.2 C cooler at comparable stations along the 145 W transect than in 1997, returning to conditions more like those in 1996. Gillnet Catches Different gilinet configurations were used along the 180, 165 W, and l45 W transects, and these configurations were similar to those used in 1996 and 1997 (Table 3; Myers et al. 1996, 1997). Salmonid sampling was conducted at 16 gillnet stations (Fig. 1; Table 4). Because of poor weather and sea conditions, there was no gillnet sampling at the 41 N station along the 180 transect. One station (44 N) on the 165 W transect was cancelled because of time lost for a medical emergency, and one station (49 N) on the 145 W transect was added because of available time. The total catch at gillnet stations was 3,265 salmonids: 978 sockeye (912 in 1997), 871 chum (506 in 1997), 822 pink (371 in 1997), 362 coho (185 in 1997), 31 chinook (0. tshawytscha; 2 in 1997), and 101 steelhead (60 in 1997). Catches of pink, coho, and chinook salmon and steelhead trout along the 145 W transect were higher than in 1997.

5 Longline Sampling and Tag Recovery Salmonid sampling was conducted at 16 longline stations (Fig. 1; Table 5). Ten salmon in the central North Pacific Ocean, 41 salmon along 165 W, and 28 salmon in the central Gulf of Alaska were tagged and released. One salmon tagged with a temperature data archival tag was released at 48 30 N, 165 W and twelve salmon were released in the central Gulf of Alaska. The serial numbers of tags released at each station are reported annually to NPAFC by FAJ, and recoveries of tagged fish and releases of thermal logger tags are reported by FRI. One double-tagged pink salmon (maturing, age 0.1) carrying a thermal logger tag and released at 145 W, 56 N on 3 July was recovered in a commercial purse seine fishery off Afognak Island (near Kodiak Island, Alaska) on 24 July 1998. One double-tagged steelhead trout (maturing, age 2.3) carrying a temperature data tag and released at 50 N, 145 W on 9 July was recovered in a commercial gillnet fishery at the delta of the Copper River, Alaska on 14 August 1998. One double-tagged coho salmon (maturing, age 1.1) carrying a thermal logger tag and released at l45 W, 56 N on 3 July was recovered in a gillnet fishery in Togiak Bay, Alaska on 24 August 1998. Fish Lacking Adipose Fins Snouts were collected from 46 salmonids lacking adipose fins (35 steelhead, 6 coho, 2 chum, 2 sockeye, and 1 chinook; Table 2C). Information on coded-wire tags which may have been found in these fish will be reported later. Scale Sampling During the Seattle port call, U.S. scientists made two sets of acetate impressions (one for FRI and one for Hokkaido Tokai University) of all scales collected by Oshoro maru personnel. The original gummed scale cards were then mailed to FAJ, National Research Institute of Far Seas Fisheries, Shimizu, for age determination and laboratory verification of species identification. Age determination and laboratory verification of species identification was also done by FRI and these data were provided to FAJ. The results of these studies will be reported later. Additional Biological Sampling Along 165 W and in the Gulf of Alaska, biological samples were collected for stock identification, food habit, growth, maturity, and migration studies (Table 2). Japanese scientists collected blood serum samples from 110 salmonids for analyses of growth and sex hormones, and heart, liver, and muscle tissue for genetic studies from 562 chum and 387 pink salmon. Otoliths for stock identification studies using thermal marks were collected by Japanese scientists from 551 chum and 386 pink salmon. A Japanese scientist also collected brain and olfactory organ samples from 214 chum and 64 pink salmon for studies of mechanisms of migration. U.S. scientists collected and analyzed stomachs from 1,077 salmonids for food habit studies. A Japanese scientist preserved samples of digestive tracts and stomach contents from 528 salmonids (up to 10 fish per species in each gillnet operation) for additional laboratory studies of pyloric cecae and food habits. The results of these studies will be reported later.

6 ACKNOWLEDGMENTS Invited cruise participants thank Dean K. Yamauchi and the Faculty of Fisheries, Hokkaido University, for the opportunity to participate in this cruise, and for the provision of Oshoro maru salmon catch, biological, and oceanographic data to the Fisheries Research Institute (FRI), University of Washington, for the preparation of this report. The officers, crew, graduate students, and cadets of the Oshoro marit are acknowledged for their outstanding assistance and cooperation in sample and data collection. Funding for FRI s participation in the cruise and for preparation of this report was provided by the Auke Bay Laboratory of the Alaska Fisheries Science Center, U.S. National Marine Fisheries Service (NOAA contract No. 5OABNF700003). REFERENCES Davis, N.D., K.W. Myers, R.V. Walker, and C.K. Harris. 1990. The Fisheries Research Institute s high-seas salmonid tagging program and methodology for scale pattern analysis. Am. Fish. Soc. Symp. 7:863-879. Fisheries Agency of Japan. 1994a. Scientific research on salmon resources should be promoted by the North Pacific Anadromous Fish Commission. (NPAFC Doc. 80.) Fisheries Agency of Japan, Tokyo, Japan. 3 p. Fisheries Agency of Japan. 1994b. A Japanese research plan for salmon stock assessment in the North Pacific Ocean. (NPAFC Doc. 81.) Fisheries Agency of Japan, Tokyo, Japan. 3 p. Myers, K.W., RN. Walker, N.D. Davis, W.S. Patton, K.Y. Aydin, E.K. Pikitch, and R.L. Burgner. 1995. Migrations, abundance, and origins of salmonids in offshore waters of the North Pacific - 1995. (NPAFC Doc. 152,) FRI-UW-9506. University of Washington, Fisheries Research Institute, Seattle. 84 p. Myers, K.W., K.Y. Aydin, and G. Anma. 1996. The 1996 international cooperative salmon research cruise of the Oshoro maru and a summary of 1994-1996 results. (NPAFC Doc. 195.) FRI-UW-9613. University of Washington, Fisheries Research Institute, Seattle. 32 p. Myers, K.W., K.Y. Aydin, G. Anma, H. Ueda, M. Kaeriyama, T. Norgard, and T. Sands. 1997. The 1997 international cooperative salmon research cruise of the Oshoro maru. (NPAFC Doc. 253.) FRI-UW-9706. University of Washington, Fisheries Research Institute, Seattle. 17 p. North Pacific Anadromous Fish Commission. 1995. Research planning and coordinating meeting. (NPAFC Doe. 121.) North Pacific Anadromous Fish Commission, 6640 NW Marine Drive, Vancouver, B.C., Canada. 14 p.

7 North Pacific Anadromous Fish Commission. 1996. Science plan 1995-96. North Pacific Anadromous Fish Commission, Vancouver, B.C., Canada. 10 p. North Pacific Anadromous Fish Commission. 1997. Science plan 1997-98. North Pacific Anadromous Fish Commission, Vancouver, B.C., Canada. 11 p. Walker, R.V. 1993. Summary of cooperative U.S.-Japan high seas salmonid research aboard the Japanese research vessel Oshoro maru, 1993. (NPAFC Doc. 21.) FRI UW-93 11. Fisheries Research Institute, University of Washington, Seattle. 16 p. Walker, R.V., and K.W. Myers. 1994. Salmonid food habits in offshore waters of the Gulf of Alaska, June-July, 1994. (NPAFC Doc. 67.) FRI-UW-9409. Fisheries Research Institute, University of Washington, Seattle. 10 p. Walker, R.V., K.W. Myers, G. Anma, and S. Sasaki. 1994. Summary of cooperative U.S.-Japan high seas salmonid research aboard the Japanese research vessel Oshoro maru, 1994. (NPAFC Doc. 66.) FRI-UW-9408. Fisheries Research Institute, University of Washington, Seattle. 15 p. Walker, R.V., K.W. Myers, N.D. Davis, H.R. Carlson, and K. Friedland. 1998. Thermal habitat of four migrating salmonids in the North Pacific Ocean and Bering Sea as recorded by temperature data tags in 1998. (NPAFC Doc.) FRI-UW-9813. Fisheries Research Institute, University of Washington, Seattle.

Figure 1. Location of fishing stations of the Oshoro mar11, 11 June to 10 July, 1998 (G=Gillnet stations, L=Longline stations). I 64 60 56 52 48 44 40 Go 140 150 160 170 180 190 200 210 1 48 V ~1I 44 1Li I I j I I I I (L-02) i.04(l-01) G-03 G-02 I (L-0~) G-0 G-0 (L-04, 05) (L-06) (L-07) G-0 (L-08) a. G-1 G-1 G-1 G-1 G-1 G-1...,, (L-10) (L-11) (L-12) (L-14) (L-15) (L-16) 40 G-01 I 140 150 160 170 180 190 200 210 220 230 240

Figure 2A. Temperature and salinity profiles of the 180 (upper and lower left) and 165 W (upper and lower right) transect lines, from CTD data. It 4 16 14 12 H CD S U ~ CD 4 0 35.0 34.5 34.0 335 (, 2 P 33.0 3a5-< 20 fl ~d 31.5 31.0 30.5-30.0 0 Latitude ( N) along 1800, June 1998 Latitude ( N) along l65 W, June 1998 39404142434445464748495045 46 47 48 49 50 51 52 53 54 l 100 200 300 0 P 0 rf CD U,-.~ a 400 500

10 Latitude ( N) along 145 W, July 1998 0 49 50 51 52 53 54 55 56 16 100 14 12 ~200 H CD 10 ~ CD 0 ~) 0 ~300 CD,, 400.4 500 49 49 0 100 50 51 52 53 54 55 56 50 51 52 53 54 55 56 0 35.0 345-34.0 ~200 33.5 CF~ D~ r~ ~ oo.u = (~i-~ 1 r~ ~300 400 500 49 50 51 52 53 54 55 56 32.01 31.5 31.0 30.5-30.0 Figure 2B. Temperature and salinity profiles of the 145 W transect line, from CTD data.

Table 1. Description of research gear and fishing operations aboard the Oshoro inaru in 1998. I Research Item/Gear j Purpose Specifications I Deployment I Sample/Data Comments carbon (POC), chla, and phytoplankton pigment. ~ A. Hydrographic Neil Brown Mark III B Used to collect CTD winch: Hydraulic Vertical haul, to 1500m, Salinity, temperature, Sigma-t, thermosteric CTD temperature, salinity, it x 72 rn/mm., 3000 m, or to bottom and dynamic depth anomaly, specific and water samples along 6.40 x 4000 m on shallow stations. anomaly were meas. at volume anomaly, the cruise track. These Work on deck was depths from 0-i500m, geopotential anomaly data have been collected completed by deck 0-3000m, or 0 - bottom. were calculated by the in the North Pacific officers, crew, cadets Water samples were shipboard computer. along the 180 transect and graduate students, collected at depth of Bucket samples since 1978, and in 1980- Water samples and data 800, 500, 300, 200, 150, collected for sea surface 81, 1994-present on the processing on the ship 125, 100, 75, 50, 30, 20, temperature and salinity. 145 W transect, was completed by 10. Water samples were graduate students. analyzed for nutrients and nitrates. Van Dorn Water To obtain large sea 20-1 Van Dorn bottle 20-I water samples were The samples were This sampling method Sampling water samples for used to collect samples collected from depth of filtered onto different allows for sampling of analysis of the chemical from particular depths. 0, 10, 30, 50, 100, 200 pore size filters and very small particles that compounds related to m. stored at -80 C. Then cannot be collected in biological actiyity. later analyzed for nets. particulate organic TSRB optical sensing To obtain light spectra TSRB equipment senses Sensor was floated Data were recorded on Data will be used in in surface waters to light levels of spectra approximately 50 m amount of light and conjunction with validate ( sea-truth ) recorded by SeaWiFS from the vessel attached spectrum. satellite data to develop SeaWiPS satellite data. satellite (412, 443, 490, by cable at daylight bio-optical algorithm for 510, 555, 670, 765, 865 oceanographic stations high latitude ocean color nm with bandwidth 20- for approximately one remote sensing. 40 nm). hour. B. Plankton Single Norpac Net Estimate biomass and Ring diameter: 0.45m; Vertical tow: 0-500 m. Samples were bottled in Copepods are the Shallow Tow 0-500m identify zooplankton. Mesh Size: 0.35 mm; Ship holds position so 5% formaldehyde and predominant taxa The time series of these Filtering Cloth: #200; the tow stays vertical, stored for biomass work collected by this gear. collections in the Gulf Length: 1.8 m Net lowered at a speed to be completed at the of Alaska are in: less then 1.0 mis and end of the cruise. 1956-62, 1980-85, retrieved at 1 mis. 1 987-present.

Research Item/Gear efficient to average out fluctuations and estimate year class strength by sampling copepods which have completed their growth phase and migrated to overwintering depth. June-July sampling provides an initial baseline. throughout the water column. I Purpose Specifications Deployment Sample/Data Comments B, Plankton (cont d.) Twin Norpac Net Estimate biomass and This is two single Vertical tow: 0-150 in. Samples were bottled in The twin Norpac allows identify zooplankton. Norpac nets held Net was lowered at a 5% formaldehyde and for collection of two This net system has together by a metal speed less than 1.0 mis, stored for hiomass work samples from the same been used periodically frame. Ring diameter: and retrieved at I mis and copepod water column. over the years. 0.45 m;mesh size (each Ship holds position so identification to be net had a different size the tow stays vertical, completed after the mesh): 0.1 mm and 0.35 cruise. mm. Each of the nets had its own flowmeter. Single Norpac Net This sampling was done Ring diameter: 0.42 m; Vertical tow: 0-1200 m. The objective is to look Estimates of growth in Deep Tow 0-1200 m to capture copepodls, Mesh Size: 0.335 mm; Net was lowered at a for spatial and temporal relation to feeding. Neocalanus spp., after Filtering Cloth: #200; speed of 0.5 mis, and variation in length as an Spatial and timing they have completed Length: 1.8 m retrieved at I m/s index of previous flucuations are most their life cycle and Flowmeter in the center Ship holds position so growth and to estimate intense during the ontogenetic down of the ring, the tow stays vertical, large-scale copepod copepod seasonal peak migration year class strength. (April-June). It is more Gamaguchi Net This net was used to Ring Diameter: 0.6 in Separate night vertical The samples are This net allows for only collect zooplankton Mesh Size: 0.1 mm. tows to: 2000-l000m, collected from specific a certain area of the samples from specific 1000-500m, 500-250m, depth areas. These water column to be areas in the water 250-thermocline, samples are stored in sampled, which in turn column. This net is like thermocline-0 rn. formaldehyde and later allows for observations a Norpac net but has a Ship holds position so analyzed for species to be made on the closing mouth. the tow stays vertical. composition. separation of species r~~z Table 1. cont d.

Table 1. cont d. I Research ItemlGear ~ I Purpose I Specifications I Deployment I Sample/Data Comments B. Plankton (cont d.) Net calibrations: This sampling was done Norpac: Ring: 0.42 m; Vertical tows: 0-150 m The samples samples The objective is to Norpac net to calibrate gear used Mesh: white 0.335 mm; during daylight. Nets are stored in provide calibration SCOR net 1956-66 to sample Length: 1.8 m were lowered formaldehyde and later factors between gear so Bongo net zooplankton at Station P Flowmeter in the center sequentially at a speed analyzed for species that historical (Norpac) with gear used of the ring, of 0.5 mis, and retrieved composition and zooplankton samples later and currently SCOR: Ring: 0.56 m; at 1 m/s. One or two biomass. can be combined with (SCOR and Bongo). Mesh: black 0.335 mm; sets of three tows done current sampling to Length: 2.8 m at selected construct a long time Flowmeter in the center oceanographic stations, series of zooplankton ~ of the ring. Ship holds position so abundance, especially Bongo: Rings: 0.56 m; the tow stays vertical, with data from Station P Mesh Size: 0.236 mm; (50 N, 145. Length: 3.4 m Flowmeter in the center of one of the rings. All nets of Nitex monofilament. C. Larval fish Larval Net (MTD net) This net was used for Mouth diameter: 1.3 m; Horizontal haul with a The larval fish samples Many species of larval the collection of larval length: 4.5 m; fish-larva net was made were preserved in fish will come to the fish found near the mesh: 3-mm mesh cloth just under the sea formaldehyde for later surface during the night. surface at night. These in the upper 300 cm, surface for 10 minutes at identification in the samples have been 0.33 mm mesh in the a speed of approx. 2 laboratory. collected for several lower 150 cm. knots. years Flowmeter was centered at mouth of the net. D. Other non-salmon Micronekton Gilinet This net is an Two 50 m panels, 6 m Set evening, No. of squid and other Net was designed to fill experimental net deep (19 mm mesh and approximately 2000 species for each panel; gap in sampling gear. designed to catch small 24 mm mesh) separated ship time; 1 hr. soak mantle length, sex, squid and other by a 50 m gap; top of before retrieval; or set micronekton net was 20 m below the overnight attached to surface salmon gillnet

I Research Item/Gear I Purpose I Specifications I Deployment I Sample/Data I Comments V D. Other non-salmon (cont d) Beam Trawl This horizontal tow Net length: 17 m; Horizontal tow for 2400 The samples were Large variety and sizes collects species cod end length :0.5 m; m, with the trawl going preserved for carbon 14 of species were throughout the water mouth opening: 2.0 m down to 800 m (400 m analysis. Carbon ratios collected from small column at sunset. on one side, 2.5 m on at later stations after will give an idea of age species such as other side, equipment problems) of the water column. copepods to large and then up. species such as octopus, approximately 20 cm in V length. E. Salmonids Research Gillnet Salmon abundance and Net configuration varied Set (ship time: sunset, No. of fish by mesh and I scale per fish from biological data for at different stations approximately 1800; species; for each mesh sockeye, chum, and ocean ecology and stock (Table 3); overall Haul (ship) time: size in C-net: fork pink; 2 scales per fish assessment; non- length: 2.45 km (49 sunrise, approximately length, sex, gonad from coho, chinook, and selective research (C) tans, 50 m/tan); depth: 0500. weight, scale(s) for up steelhead (I scale from net introduced in 1971; approx. 6 m; hydraulic to 100 fish of each each side of body). systematic surveys with net hauler: 0.3 t x 177 species (body weight for gilinet for abundance rn/mm. up to 60 fish); A-net estimation commenced (commercial meshes): in 1972, 145 W transect same data as C-net in 1980-8 1, 1994-1998; except in 1998 only 30 165 W transect in 1998. fish of each species sampled per mesh. Surface Longline tagged with temperature V Live capture of fish for No. hachi (basket) per Set and haul (ship) times No. of fish by species; Fish are double-tagged high seas tagging operation varied in 1998 varied in 1998 (see mortalities: fork length, with two red and white, research; long time (Table 5); hachi Table 5). body wt., scale(s); approx. 1.6-2.0cm series of data 1955- mainline: 127 m long; viable fish; fork length, Petersen disk tags (one present in North Pacific. 34 branch lines/hachi; 3 scale(s), tag nos. Japan tag, one FRI tag) m between branch lines; attached to the fish in fishing depth: approx. 2 front of the dorsal fin m; bait: small salted with a plastic cinch. anchovy. Selected fish were also data tags (see text). Table 1. cont d.

15 Table 2. Additional salmonid research activities conducted aboard the Oshoro maru in 1998. Sample Fishing Data or Subject (no. collected) Gear Method Samples collected A. Salmonid Food Habits and Feeding Ecology Stomach Stomachs Gillnet and Stomachs from Prey weight, % contents examined longline esophagus to composition by from 1,077 pyloric valve, or volume of each prey salmonids; entire digestive type, fullness and stomachs and tract, collected digestion indices; digestive tracts and examined on specimens of prey for of up to 10 ship from up to caloric content fish per 20 fish of each analysis (frozen); species in each species in each preserved contents operation gilinet operation identified and counted preserved in later 10% formaldehyde B. Salmonid Ocean Growth, Maturity, and Mechanisms of Migration. Growth and Blood serum Gilinet Blood drawn Accompanying scale maturity samples: 50 from caudal vein; samples, biological, indices and sockeye, 50 centrifuged at and oceanographic physiological chum, 5 coho, 3000 rpm for 15 data studies 5 steelhead mm; 1.0 ml in each of two 1.5 ml cryo-tubes; frozen at -80 C Phospholipid Brain and Gilinet Samples frozen Accompanying analysis and olfactory at -80 C biological and morphological tissue samples oceanographic data studies of from 214 olfactory chum and 64 epithelium and pink salmon CNS

16 Table 2. cont d. Sample Fishing Data or Subject (no. collected) Gear Method Samples collected B. Salmonid Ocean Growth, Maturity, and Mechanisms of Migration (cont d) Temperature- Tagged fish Longline Viable maturing Thermal tag nos. 8, recording (13 live fish: 4 salmonids in 28, 52, 157, 158, 164, archival data sockeye, 1 longline catches 167, 184, 189, 191, tagging chum, 4 pink, were tagged with 196, 198, 199 3 coho, 1 thermal logger steelhead) tags as well as Japan and FRI disk tags C. Stock Identification Genetic stock Muscle, liver, Gillnet Samples frozen Accompanying identification and heart for isozyme biological and collected from analysis oceanographic data 562 chum and 387 pink salmon High seas Snouts from Gillnet and All fish in the Snouts (frozen) and coded-wire tag fish lacking longline catch were accompanying catch, recovery the adipose fin (mortalities) examined data, biological data, (35 steelhead, and scale samples; 6 coho, 2 snouts shipped to U.S. chum, 2 NMFS, Auke Bay sockeye, 1 chinook) Laboratory, Juneau, for tag detection and decoding ~ Double Tagged fish Longline All viable Tag nos., Japan/FRI: tagging (79 live fish) salmonids in DD6O1-05, DD6507- experiments longline catches 8, DD6509- were double- 8OILL1 109-80 tagged with Japan and FRI tags

17 Table 2. cont d. Sample Fishing Data or Subject (no. collected) Gear Method Samples collected C. Stock Identification (cont d) High seas Otoliths from Gillnet Otoliths cleaned Accompanying recovery of 551 chum and and preserved biological data, and marked 386 pink dry scale samples; otoliths otoliths salmon taken to National Salmon Resource Center, Sapporo, for mark detection and decoding Taxonomic Intestinal Gilinet and Entire digestive Accompanying scale studies of tracts from longline tract, or pyloric samples, stomach pyloric cecae 528 valve to end of contents, biological salmonids; pyloric cecae, and oceanographic entire collected from up data digestive tracts to 10 fish of each of up to 10 species in each fish per gillnet operation; species in each preserved in 10% operation formaldehyde

18 Table 3. Gillnet configurations used in 1998. 1 tan = 50-rn long section of gilinet. Size 180 and 165 W 145 W Type* (mm) No. tans No. tans A 115 6 9 F 19 1 0 F 29 1 0 F 37 1 0 C 48 3 3 C 93 3 3 C 157 3 3 C 106 3 3 C 63 3 3 C 121 3 3 C 72 3 3 C 138 3 3 C 82 3 3 C 55 3 3 F 42 1 0 F 33 1 0 F 25 1 0 F 22 1 0 A 121 6 10 Total 49 49 * A = traditional salmon commercial gilinet C = salmon research gilinet F = experimental gilinet (non-salmon research)

19 Table 4. Salmon caught in 1998 Oshoro Maru gillnet operations. Location, sea surtace temperature (SST, C), surface salinity (psu, practical salinity units) number of 50-m tans and catch by research-mesh and commercial-mesh gilinet for each fishing station. Recovery SST Station Date Location Salin. Gear Tans Sockeye Chum Pink Coho Chinook Steeihead Total Satmon G-01 11-Jun-98 39 OON 14.5 Commercial 12 o o 0 0 0 0 0 180 00 33.98 Research 30 o o 0 0 0 0 0 Totai 42 o o 0 0 0 0 0 G-02 13-Jun-98 43 00 N 9.0 Commercial 12 o 0 0 37 0 5 42 180 00 33.32 Research 30 o 18 0 20 0 6 44 Total 42 0 18 0 57 0 11 86 G-03 14-Jun-98 45 00 N 7.9 Commercial 12 0 0 0 4 0 2 6 180 00 32.89 Research 30 4 19 1 4 0 4 32 Total 42 4 19 1 8 0 6 38 G-04 15-Jun-98 47 00 N 5.9 Commercial 12 3 0 1 3 0 1 8 180 00 32.06 Research 30 72 43 5 2 0 1 123 Total 42 75 43 6 5 0 2 131 180 Transect Totals Commercial 3 0 1 44 0 8 56 Research 76 80 6 26 0 II 199 Total 79 80 7 70 0 19 255 G-05 26-Jun-98 50 00 N 7.2 Commercial 12 22 57 165 OOW 32.18 Research 30 78 82 Total 42 100 139 G-06 27-Jun-98 48 30 N 8.4 Commercial 12 12 20 165 00 W 32.40 Research 30 31 32 Total 42. 43 52 G-07 28-Jun-98 47 00 N 8.9 Commercial 12 3 15 165 00 W 32.29 Research 30 20 26 Total 42 23 41 G-08 29-Jun-98 45 30 N 9.5 Commercial 12 3 5 165 OOW 32.51 Research 30 9 68 Total 42 12 73 165 W Transect Totals Commercial 40 97 Research 138 208 178 305 G-09 3-Jul-98 56 00 N 10.9 Commercial 19 51 45 145 00W 32.29 Research 30 36 36 Total 49 87 81 G-10 4-Jul-98 55 00 N 10.4 Commercial 19 30 22 145 00W 32.19 Research 30 19 23 Total 49 49 45 G-11 5-Jul-98 54 OON 9.9 Commercial 19 93 58 145 00W 32.60 Research 30 68 44 Total 49 161 102 G-12 6-Jul-98 53 00 N 9.5 Commercial 19 79 20 145 00W 32.30 Research 30 40 24 Total 49 119 44 G-13 7-Jul-98 52 00N 9.9 Commercial 19 31 68 145 OOW 32.25 Research 30 13 61 Total 49 44 129 G-14 8-Jul-98 51 00 N 10.4 Commercial 19 35 6 145 00W 32.60 Research 30 15 29 Total 49 50 35 G-15 9-Jul-98 50 00N 10.9 Commercial 19 73 2 145 00W 32.50 Research 30 68 9 Total 49 141 11 G-16 10-Jul-98 49 OON 10.6 Commercial 19 46 4 145 OOW 32.49 Research 30 24 34 Total 49 70 38 145 WTransect Commercial 438 225 Research 283 260 Total 721 485 10 0 14 0 24 0 2 0 5 0 7 0 9 3 23 5 32 8 8 27 13 26 21 53 29 30 55 31 84 61 0 0 89 0 0 174 0 0 263 2 3 39 0 3 71 2 6 110 2 0 32 1 0 75 3 0 107 1 2 46 0 2 118 1 4 164 5 5 206 1 5 438 6 10 644 103 44 0 2 245 71 13 0 2 158 174 57 0 4 403 42 36 0 0 130 54 30 0 6 132 96 66 0 6 262 64 22 12 3 252 47 12 5 5 181 111 34 17 8 433 85 24 3 9 220 40 17 2 9 132 125 41 5 18 352 41 8 2 1 151 18 3 0 12 107 59 11 2 13 258 47 9 1 3 101 31 5 0 7 87 78 14 1 10 188 14 1 0 2 92 4 1 0 6 88 18 2 0 8 180 57 4 0 2 113 12 2 0 3 75 69 6 0 5 188 453 148 18 22 1304 277 83 7 50 960 730 231 25 72 2264. Total Salmon Catch Sockeye Chum Pink Coho Chinook Steelhead Total Stations G-01 -G-16 Commercial 481 322 483 222 23 35 1566 Research 497 548 338 140 8 66 1597 Total 978 870 821 362 31 101 3163

Table 5. Salmonids tagged and released during 1998 Oshoro maru longline operations. Location, sea surface temperature (SST, C), surface salinity (psu, practical salinity units), and catch tagged and released. S.M.T. = Ship Mean Time; hachi = unit of longline gear (34 hooks per hachi). Station Date Time (S.M.T.) Latitude Longitude SST Salin. Hachi Sockeye Chum Pink Coho. Chinook Steelhead Total L-01 6/16/98 04:20-06:45 46 59 N 179 58 W 5.9 32.06 10 0 7 0 0 0 0 7 L-02 6/17/98 14:40-17:50 50 05 N 179 55 W 6.0 32.25 8 0 3 0 0 0 0 3 L-03 6/23/98 07:00-10:20 52 40 N 165 00 W 7.9 31.55 10 0 0 0 0 0 0 0 L-04 6/25/98 06:40-09:25 50 02 N 164 58 W 7.3 32.18 10 0 1 0 0 0 0 1 L-05 6/26/98 04:45-07:30 49 59 N 164 56 W 7.3 32.18 10 0 3 0 0 0 0 3 L-06 6/27/98 04:40-06:50 48 29 N 164 59 W 8.2 32.40 10 1 2 0 0 0 0 3 L-07 6/28/98 04:55-07:00 47 00 N 164 59 W 8.4 32.29 10 1 4 1 0 0 0 6 L-08 6/29/98 04:50-07:15 45 30 N 164 59 W 9.6 32.51 10 0 20 4 4 0 0 28 L-09 7/3/98 04:20-07:45 55 59N 145 OOW 10.9 32.29 10 1 4 6 3 0 0 14 L-10 7/4/98 04:25-07:10 54 59N 145 OOW 10.5 32.19 10 2 1 4 0 0 0 7 L-11 7/5/98 04:25-07:30 53 59N 145 OOW 9.9 32,60 10 0 0 1 0 0 0 1 L-12 7/6/98 04:25-07:05 53 01 N 144 53 W 9.5 32.30 10 0 0 1 1 0 0 2 L-13 7/7/98 04:25-06:55 52 01 N 144 58 W 10.0 32.35 10 0 1 0 0 0 0 L-14 7/8/98 04:25-07:35 50 59 N 144 58 W 10.3 32.60 10 0 1 0 0 0 0 1 L-15 7/9/98 04:30-07:15 49 58N 144 58W 10.4 32.35 10 0 0 1 0 0 1 2 L-16 7/10/98 04:25-07:15 48 58N 145 OIW 10.6 32.49 10 0 0 0 0 0 0 0 Total 5 47 18 8 0 1 79 C