Rock Creek Huntington County Supplemental Evaluation

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Rock Creek Huntington County Supplemental Evaluation Date of Survey: September 8 to September 10, 2008 Biologist: Rod A. Edgell Survey Objectives: Conduct population estimates for game fish at Rock Creek as described in Work Plan 300FW1F10D41635. Methods: Fish collection effort consisted of pulsed D.C. day electrofishing with a tote barge at four stations along Rock Creek located on J.E. Roush Lake property (Figure 1). The three-pass depletion method was used with block nets at either end of the station. Stations were sampled in an upstream direction and all game fish were collected and removed until three passes had been completed. Total length of all game fish was measured to the nearest 0.1 in and weight was measured to the nearest 0.01 lbs. Five scales per half-inch group were collected for age determination and back-calculated lengths-at-age. Length frequency distribution for reporting purposes will be grouped in half-inch groups which are defined as X.0 X.4 and X.5 X.9. Age-length keys were also constructed to determine mean length-at-age. Proportional stock densities (PSD) were calculated for both smallmouth bass and rock bass, while relative stock densities (RSD) of 12 and 15 in fish were calculated for smallmouth bass only. All stock densities were calculated using electrofishing catch only (Anderson and Neumann 1996). Population estimates were not calculated for all game fish due to low catch rates or irregular removal patterns. Population estimates were determined using MicroFish 3.0 software (MicroFish Interactive Program, J.S. Van Deventer and W. S. Platts, Microsoft Corportation, 1986). Summary: A total of 484 fish, weighing 168.48 lbs was collected during this survey (Table1). Smallmouth bass was the most abundant species collected by number (77%), followed 1

by rock bass (18%), and bluegill (2%). Other species collected include largemouth bass and channel catfish. A total of 374 smallmouth bass was collected at Rock Creek (Table 1). Total length of smallmouth bass ranged from 2.3 to 18.6 in. The PSD, RSD-12, and RSD-15 for smallmouth bass during this survey were 43, 26, and 6, respectively (Table 2). Of the smallmouth bass collected 3% were greater than or equal to 15 in, and the electrofishing catch rate of these fish was 3.6 fish/h. Of the smallmouth bass collected in 2006 1% were greater than or equal to 15 in, and the electrofishing catch rate of these fish was 0.9 fish/h (Braun and Grier 2006). Based on the age length key and back calculated lengths at age the majority of smallmouth bass reach 12 in between ages 3 4 and reach 15 in between ages 5 6. Growth of smallmouth bass in 2008 was comparable to rates observed in 2006. The population estimates (confidence intervals; standard error) for smallmouth bass were calculated for each station: station 1 was 49 fish (47 51 fish; SE = 0.866), station 2 was 118 fish (111 125 fish; SE = 3.445), station 3 was 97 fish (89 105 fish; SE = 4.254), and station 4 was 125 fish (120 130 fish; SE = 2.558) (Table 2). The population estimates were expanded to the number of fish per mile for each station: station 1 (440 ft) was 588 fish/mile (567 612 fish; SE = 10.392), station 2 (823 ft) was 757 fish/mile (713 801 fish; SE = 22.101), station 3 (697 ft) was 735 fish/mile (671 799 fish; SE = 32.225), and station 4 (685 ft) was 964 fish/mile (924 1003 fish; SE = 19.717). The greatest differences in population estimates from 2006 to 2008 were observed at stations 2 and 4 (Table 2). Despite these differences the overall number of smallmouth bass collected during the two surveys was nearly equal. A total of 86 rock bass was collected at Rock Creek. Total length of rock bass ranged from 1.6 to 8.6 in. The PSD for rock bass during this survey was 22. Of the rock bass collected 14% were greater than or equal to 7 in. Based on the age length key and back calculated lengths at age the majority of rock bass reach 7 in by age 3. The population estimates (confidence intervals; standard error) for rock bass were calculated for two stations: station 2 was 12 fish (6 18 fish; SE = 2.542), and station 3 was 42 fish (32 52 fish; SE = 4.943). The population estimates were expanded to the number of fish per mile for each station: station 2 (823 ft) was 77 fish/mile (41 113 fish; SE = 16.308), and station 3 (697 ft) was 318 fish/mile (243 394 fish; SE = 37.444). Population estimates were not calculated for rock bass in 2006. 2

Recommendations: A property rule prohibiting the harvest of black bass from Rock Creek was implemented in the fall of 2008. This survey should be duplicated in 2010 as outlined in the aforementioned work plan to asses any changes as a result of the new regulation. Literature cited: Anderson, R. O., and R. M. Neumann. 1996. Length, weight, and associated structural indices. Pages 447-481 in B. R. Murphy and D. W. Willis, editors. Fisheries techniques, 2 nd edition. American Fisheries Society, Bethesda, Maryland. Braun, E. R., and A. C. Grier. 2006. Smallmouth bass population estimation in Rock Creek, Huntington County Wildlife Management Area, Supplemental Evaluation. Indianapolis, Indiana. Submitted by: Rod A. Edgell, Assistant Biologist Date: 10/22/2008 Approved by: Edward R. Braun, Biologist Date: 10/24/2008 Approved by: Stuart T. Shipman, Fisheries Supervisor Date: 3/25/2009 3

Station 1 Station 2 Station 3 Station 4 Figure 1. Electrofishing sampling locations at Rock Creek, Huntington County, Indiana in September 2008. 4

Table 1. Relative abundance, length range (in), and relative weight of species collected at 4 sampling stations on Rock Creek, Huntington County Indiana in September 2008. Overall Species Number % Length range Weight % Smallmouth bass 374 77.3 2.3-18.6 137.94 81.9 Rock bass 86 17.8 1.6-8.6 7.72 4.6 Bluegill 9 1.9 4.2-6.2 0.75 0.4 Largemouth bass 8 1.7 3.0-15.4 2.99 1.8 Channel Catfish 7 1.4 9.3-27.9 19.08 11.3 Total 484 168.48 Station 1 Species Number % Length range Weight % Smallmouth bass 49 89.1 4.4-9.8 6.86 90.5 Largemouth bass 6 10.9 3.0-9.3 0.72 9.5 Total 55 7.58 Station 2 Species Number % Length range Weight % Smallmouth bass 113 87.6 2.4-16.0 39.29 97.7 Rock bass 11 8.5 1.6-7.0 0.66 1.6 Bluegill 4 3.1 4.3-4.9 0.24 0.6 Largemouth bass 1 0.8 3.8 0.02 0.0 Total 129 40.21 Station 3 Species Number % Length range Weight % Smallmouth bass 91 71.1 2.3-15.5 24.58 92.0 Rock bass 37 28.9 1.7-7.0 2.14 8.0 Total 128 26.72 Station 4 Species Number % Length range Weight % Smallmouth bass 121 70.3 2.7-18.6 67.21 71.5 Rock bass 38 22.1 1.8-8.6 4.92 5.2 Channel catfish 7 4.1 9.3-27.9 19.08 20.3 Bluegill 5 2.9 4.2-6.2 0.51 0.5 Largemouth bass 1 0.6 15.4 2.25 2.4 Total 172 93.97 5

Table 2. Number, proportional stock density (PSD), relative stock density 15 (RSD-15), pecent greater than or equal to 15 in (% 15), catch per unit effort greater than or equal to 15 in (CPUE 15), estimated population, and electrofishing effort (h) of smallmouth bass collected at 4 sampling stations on Rock Creek, Huntington County in September 2006 and 2008. 2006 Number PSD RSD - 15 % 15 CPUE 15 Pop Estimate Effort Overall 376 31 2 1 0.91 NA 3.29 Station 1 64 0 0 0 0.00 68 0.41 Station 2 164 39 5 2 2.56 166 1.17 Station 3 110 38 0 0 0.00 112 0.86 Station 4 38 25 0 0 0.00 39 0.87 2008 Number PSD RSD - 15 % 15 CPUE 15 Pop Estimate Effort Overall 374 43 6 3 3.60 NA 3.33 Station 1 49 0 0 0 0.00 49 0.46 Station 2 113 28 2 2 2.60 118 0.77 Station 3 91 26 2 1 1.23 97 0.81 Station 4 121 57 10 7 6.98 125 1.29 6

Appendix 7

Lake: Rock Creek All Stations TN GN EF Date: 9/8/2008 to 9/10/2008 Total # 0 0 374 Species: Smallmouth bass Effort 0 0 3.33 Total number: 374 CPUE NA NA 112 Total weight: 137.94 Length range: 2.3 to 18.6 Group TL (in) TN GN EF TOTAL RSD Stock 7 0 0 201 201 - Quality 11 0 0 87 87 43 Preferred 14 0 0 19 19 9 Memorable 17 0 0 2 2 1 Trophy 20 0 0 0 0 Length Mean Length Mean Length Mean group (in) # weight (lbs) group (in) # weight (lbs) group (in) # weight (lbs) 1.0 17.5 1 2.30 34.0 1.5 18.0 34.5 2.0 4 0.01 18.5 1 2.94 35.0 2.5 23 0.01 19.0 35.5 3.0 8 0.01 19.5 36.0 3.5 2 0.02 20.0 36.5 4.0 1 0.04 20.5 37.0 4.5 7 0.05 21.0 37.5 5.0 36 0.06 21.5 38.0 5.5 38 0.08 22.0 38.5 6.0 31 0.11 22.5 39.0 6.5 23 0.13 23.0 39.5 7.0 19 0.16 23.5 40.0 7.5 19 0.20 24.0 40.5 8.0 9 0.23 24.5 41.0 8.5 9 0.28 25.0 41.5 9.0 8 0.34 25.5 42.0 9.5 10 0.42 26.0 42.5 10.0 20 0.45 26.5 43.0 10.5 20 0.56 27.0 43.5 11.0 20 0.62 27.5 44.0 11.5 15 0.73 28.0 44.5 12.0 9 0.82 28.5 45.0 12.5 10 0.91 29.0 45.5 13.0 9 1.03 29.5 46.0 13.5 5 1.13 30.0 46.5 14.0 6 1.33 30.5 47.0 14.5 1 1.38 31.0 47.5 15.0 3 1.54 31.5 48.0 15.5 3 1.81 32.0 48.5 16.0 3 1.87 32.5 49.0 16.5 1 2.22 33.0 49.5 17.0 33.5 50.0 8

Back-calculated lengths-at-age for smallmouth bass captured at Rock Creek, Huntington County, Indiana in September 2008. Age Year Class # Aged I II III IV V VI VII VIII 2007 40 4.0 SD 1.0 2006 13 3.5 6.3 SD 0.7 1.3 2005 26 3.5 5.8 9.2 SD 0.5 1.0 1.3 2004 10 3.1 6.5 9.5 11.8 SD 0.5 0.7 0.8 0.8 2003 4 3.4 5.8 8.7 10.9 13.3 SD 0.2 0.3 1.0 1.2 1.1 2002 3 3.1 5.6 9.1 11.9 13.3 14.4 SD 0.2 0.5 0.6 1.0 0.8 0.7 2001 1 2.6 5.6 9.5 11.7 13.4 14.9 15.8 SD 0.0 0.0 0.0 0.0 0.0 0.0 0.0 2000 1 3.5 5.7 9.4 13.1 15.3 16.8 17.9 18.2 SD 0.0 0.0 0.0 0.0 0.0 0.0 0.0 0.0 Mean* 3.4 6.0 9.1 11.5 13.3 14.4 SD 0.5 0.8 0.9 1.0 1.0 0.7 9

Age-length key for smallmouth bass captured at Rock Creek, Huntington County, Indiana in September 2008. Length # in # (age) in Age Group sample subsample 1 2 3 4 5 6 7 8 2.0 4 3(0) 2.5 23 5(0) 3.0 8 3(0) 3.5 2 2(0) 4.0 1 1(1) 1 4.5 7 4(1) 7 5.0 36 5(1) 36 5.5 38 4(1), 1(2) 30 8 6.0 31 5(1) 31 6.5 23 4(1) 23 7.0 19 5(1) 19 7.5 19 5(1) 19 8.0 9 3(1), 2(2) 5 4 8.5 9 2(1), 3(2) 4 5 9.0 8 3(2), 1(3) 6 2 9.5 10 1(1), 1(2), 2(3) 2 3 5 10.0 20 2(2), 3(3) 8 12 10.5 20 1(2), 4(3) 4 16 11.0 20 5(3) 20 11.5 15 4(3), 1(4) 12 3 12.0 9 3(3), 1(4) 7 2 12.5 10 3(3), 1(4) 7 3 13.0 9 4(4) 9 13.5 5 1(3), 1(4), 1(5) 1 2 2 14.0 6 2(4), 1(5) 4 2 14.5 1 15.0 3 1(5), 1(6) 2 1 15.5 3 1(6) 3 16.0 3 1(5), 1(6) 1 2 16.5 1 1(7) 1 17.0 17.5 1 18.0 18.5 1 1(8) 1 Mean TL 6.4 8.8 11.2 13.1 14.8 15.8 16.8 18.8 SE 0.1 0.3 0.1 0.2 0.4 0.2 10

Lake: Rock Creek All Stations TN GN EF Date: 9/8/2008 to 9/10/2008 Total # 0 0 86 Species: Rock bass Effort 0 0 3.33 Total number: 86 CPUE NA NA 26 Total weight: 7.72 Length range: 1.6 to 8.6 Group TL (in) TN GN EF TOTAL RSD Stock 4 0 0 55 55 - Quality 7 0 0 12 12 22 Preferred 9 0 0 0 0 Memorable 11 0 0 0 0 Trophy 13 0 0 0 0 Length Mean Length Mean Length Mean group (in) # weight (lbs) group (in) # weight (lbs) group (in) # weight (lbs) 1.0 17.5 34.0 1.5 3 0.00 18.0 34.5 2.0 18.5 35.0 2.5 19.0 35.5 3.0 1 0.03 19.5 36.0 3.5 27 0.04 20.0 36.5 4.0 31 0.05 20.5 37.0 4.5 7 0.06 21.0 37.5 5.0 3 0.08 21.5 38.0 5.5 22.0 38.5 6.0 1 0.16 22.5 39.0 6.5 1 0.18 23.0 39.5 7.0 4 0.27 23.5 40.0 7.5 3 0.32 24.0 40.5 8.0 4 0.39 24.5 41.0 8.5 1 0.45 25.0 41.5 9.0 25.5 42.0 9.5 26.0 42.5 10.0 26.5 43.0 10.5 27.0 43.5 11.0 27.5 44.0 11.5 28.0 44.5 12.0 28.5 45.0 12.5 29.0 45.5 13.0 29.5 46.0 13.5 30.0 46.5 14.0 30.5 47.0 14.5 31.0 47.5 15.0 31.5 48.0 15.5 32.0 48.5 16.0 32.5 49.0 16.5 33.0 49.5 17.0 33.5 50.0 11

Back-calculated lengths-at-age for rock bass captured at Rock Creek, Huntington County, Indiana in September 2008. Age Year Class # Aged I II III 2007 13 2.4 SD 0.2 2006 3 2.1 4.1 SD 0.2 0.7 2005 8 2.4 4.4 7.6 SD 0.2 0.3 1.3 Mean* 2.3 4.3 7.6 SD 0.2 0.5 1.3 Age-length key for rock bass captured at Rock Creek, Huntington County, Indiana in September 2008. Length # in # (age) in Age Group sample subsample 1 2 3 1.5 3 3(0) 2.0 2.5 3.0 1 3.5 27 5(1) 27 4.0 31 4(1) 31 4.5 7 4(1) 7 5.0 3 1(2) 3 5.5 6.0 1 1(2) 1 6.5 1 1(2) 1 7.0 4 2(3) 4 7.5 3 2(3) 3 8.0 4 3(3) 4 8.5 1 1(3) 1 Mean TL 4.1 5.8 7.8 SE 0.0 0.3 0.1 12