Montgomery Parks Biological Monitoring in the Anacostia Watershed of Montgomery County RESOURCE ANALYSIS SECTION

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Montgomery Parks Biological Monitoring in the Anacostia Watershed of Montgomery County PARK PLANNING & STEWARDSHIP Doug Redmond, Natural Resources Manager Montgomery County Department of Parks September 13, 2012 The Anacostia Watershed Anacostia drainage in Montgomery County comprises 61 square miles Accounts for 1/3 of the drainage area to the Anacostia River MWCOG Partnership 2 1

Index of Biological Integrity (IBI) Biological Monitoring The diversity of fish and benthic macroinvertebrates (aquatic bugs) are studied within a 75 meter transect. Those data are averaged and used to create an IBI that indicates water quality as being excellent, good, fair or poor. Scoring Criteria for Fish IBI Metrics Scoring Criteria (a) Metric (b) 5 3 1 1. Total number of fish species >7 4-7 <4 2. Number of riffle benthic insectivorous individuals >13 7-13 <7 3. Number of minnow species (Cyprinidae) >4 3-4 <3 4. Number of intolerant species >1 1 0 5. Proportion of tolerant individuals <60% 60-80% >81% 6. Proportion of individuals as omnivores/generalists <55% 55-77% >77% 7. Proportion of individuals as insectivores >39% 20-39% <20% 8. Proportion of individuals as pioneering species <53% 53-76% >76% 9. Total number of individuals (excluding tolerants sp.) >50 26-50 <26 10. Proportion with disease/anomalies <2.2% 2.2-4.1% >4.1% (a) Scoring Criteria are based on the 1995 and 1996 reference streams. (b) Metrics are based on Karr et al. (1986) original scoring criteria, and modifications from Plafkin et al. (1989) and Hall et al. (1993). Total IBI Score Rating 46 50 Excellent 34 45 Good 22 33 Fair 10 21 Poor 3 Annual Monitoring Conducted by Montgomery Parks for MWCOG SCSC314 & NWW421 NWNW421 SCSC314 4 2

SCSC314 Species 2007 2008 2009 2010 2012 American eel Satinfin shiner Spotfin shiner Silverjaw minnow Swallowtail shiner Bluntnose minnow Blacknose dace Longnose dace Creek chub White sucker Brown bullhead Redbreast sunfish Green sunfish Bluegill Largemouth bass Tessellated darter FIBI SCORE 20 (Poor) 20 (Poor) 24 (Fair) 28 (Fair) 28 (Fair) American Eel Creek Chub Redbreast Sunfish 5 Silverjaw Minnow SCSC314 Species 2007 2008 2009 2010 2012 American eel Satinfin shiner Spotfin shiner Silverjaw minnow Swallowtail shiner Bluntnose minnow Blacknose dace Longnose dace Creek chub White sucker Brown bullhead Redbreast sunfish Green sunfish Bluegill Largemouth bass Tessellated darter FIBI SCORE 20 (Poor) 20 (Poor) 24 (Fair) 28 (Fair) 28 (Fair) American Eel Creek Chub Redbreast Sunfish 6 Silverjaw Minnow 3

SCSC314 Species 2007 2008 2009 2010 2012 American eel Satinfin shiner Spotfin shiner Silverjaw minnow Swallowtail shiner Bluntnose minnow Blacknose dace Longnose dace Creek chub White sucker Brown bullhead Redbreast sunfish Green sunfish Bluegill Largemouth bass Tessellated darter FIBI SCORE 20 (Poor) 20 (Poor) 24 (Fair) 28 (Fair) 28 (Fair) American Eel Creek Chub Redbreast Sunfish 7 Silverjaw Minnow Smallmouth Bass Species 2007 2008 2009 2012 NWNW421 Sea Lamprey American eel Central stoneroller Satinfin shiner Spotfin shiner Cutlips minnow E. silvery minnow Common shiner Bluegill Yellow Bullhead Silverjaw minnow Spottail shiner Swallowtail shiner Bluntnose minnow Blacknose dace Longnose dace Creek chub White sucker Creek chubsucker Northern hogsucker Yellow bullhead Margined madtom Banded Killifish Redbreast sunfish Green sunfish Pumpkinseed Bluegill Smallmouth bass Largemouth bass Fantail darter Tessellated darter Pumpkinseed x Redbreast Hybrid sunfish FIBI SCORE 26 (Fair) 34 (Good) 24 (Fair) 28 (Fair) 8 4

Smallmouth Bass Species 2007 2008 2009 2012 NWNW421 Sea Lamprey American eel Central stoneroller Satinfin shiner Spotfin shiner Cutlips minnow E. silvery minnow Common shiner Bluegill Yellow Bullhead Silverjaw minnow Spottail shiner Swallowtail shiner Bluntnose minnow Blacknose dace Longnose dace Creek chub White sucker Creek chubsucker Northern hogsucker Yellow bullhead Margined madtom Banded Killifish Redbreast sunfish Green sunfish Pumpkinseed Bluegill Smallmouth bass Largemouth bass Fantail darter Tessellated darter Pumpkinseed x Redbreast Hybrid sunfish FIBI SCORE 26 (Fair) 34 (Good) 24 (Fair) 28 (Fair) 9 10 5

Best Natural Areas Areas of parkland which contain one or more of the following: Large areas of contiguous, high quality forest and/or wetland which are generally more than 100 acres, and show little evidence of past land use disturbance. Rare, threatened, endangered, or watch list species. The best examples of notable plant communities found in Montgomery County. High quality wetlands, including those of Special State Concern as noted in COMAR, Title 26. Aquatic communities rated as good or excellent in the Countywide Stream Protection Strategy. Special Trout Management Areas as noted in COMAR, Title 08. Areas of exceptional scenic beauty. 11 Biodiversity Areas Areas of parkland which contain one or more of the following: Areas of contiguous, high quality forest and/or wetland which show little evidence of past land use disturbance. Rare, threatened, endangered, or watch list species. Exceptional examples of notable plant community types found in Montgomery County. Areas of exceptional scenic beauty. 12 6

Best Natural Areas and Biodiversity Areas in Parks Best Natural Areas (9,366.4 acres) Biodiversity Areas (4,512.9 acres) M NCPPC Parks Other Parks 13 2010 Montgomery Parks Biological Monitoring Sites Northern Hogsucker 14 7

2010 Montgomery Parks Biological Monitoring Sites Northern Hogsucker PBPB311 PBWF102 SCFT102a & SCFT102b 15 2010 Montgomery Parks Biological Monitoring Sites Northern Hogsucker PBPB311 PBWF102 Excellent Good SCFT102a & SCFT102b Fair Poor 16 8

2010 Montgomery Parks Biological Monitoring Sites Sea Lamprey Species SCFT102a SCFT102b PBWF102 PBPB311 American eel Rosyside dace Cutlips minnow Common shiner Blacknose dace Longnose Dace Longnose dace Creek chub Fallfish White sucker Northern hogsucker Margined madtom Blue Ridge sculpin Green sunfish Tessellated darter FIBI SCORE 14 (Poor) 20 (Poor) 22 (Fair) 30 (Fair) Roseyside Dace Fallfish 17 2010 Montgomery Parks Biological Monitoring Sites Species SCFT102a SCFT102b PBWF102 PBPB311 Sea Lamprey American eel Rosyside dace Cutlips minnow Common shiner Blacknose dace Longnose dace Creek chub Fallfish White sucker Northern hogsucker Margined madtom Blue Ridge sculpin Green sunfish Tessellated darter FIBI SCORE 14 (Poor) 20 (Poor) 22 (Fair) 30 (Fair) 18 9

2011 Montgomery Parks Biological Monitoring Sites Creek Chub 19 2011 Montgomery Parks Biological Monitoring Sites Creek Chub NWLR102 20 10

2011 Montgomery Parks Biological Monitoring Sites Creek Chub NWLR102 Excellent Good Fair Poor Species Blacknose Dace Creek Chub FIBI SCORE NWLR102 14 (Poor) 21 2012 Montgomery Parks Biological Monitoring Sites Smallmouth Bass 22 11

2012 Montgomery Parks Biological Monitoring Sites Smallmouth Bass PBUT201 NWNW413 NWND101 NWNW421 SCSC314 23 2012 Montgomery Parks Biological Monitoring Sites Smallmouth Bass PBUT201 NWNW413 Excellent NWND101 Good Fair NWNW421 Poor SCSC314 24 12

2012 Montgomery Parks Biological Monitoring Sites Species PBUT201 NWNW413 NWND101 Satinfin shiner Cutlips minnow Common shiner Silverjaw minnow Spottail shiner Swallowtail shiner Bluntnose minnow Blacknose dace Longnose dace Creek chub White sucker Northern hogsucker Margined madtom E. Mosquitofish Redbreast sunfish Green sunfish Pumpkinseed Bluegill Smallmouth bass Largemouth bass Fantail darter Tessellated darter FIBI SCORE 0 (Poor) 32 (Fair) 22 (Fair) Fantail Darter Pumpkinseed Largemouth Bass Eastern Mosquitofish 25 Green Sunfish White Sucker 2012 Montgomery Parks Biological Monitoring Sites Species PBUT201 NWNW413 NWND101 Satinfin shiner Cutlips minnow Common shiner Silverjaw minnow Spottail shiner Swallowtail shiner Bluntnose minnow Blacknose dace Longnose dace Creek chub White sucker Northern hogsucker Margined madtom E. Mosquitofish Redbreast sunfish Green sunfish Pumpkinseed Bluegill Smallmouth bass Largemouth bass Fantail darter Tessellated darter FIBI SCORE 0 (Poor) 32 (Fair) 22 (Fair) 26 13

Questions? 27 RAW DATA 28 14

SCSC314 IBI DATA (2007 & 2008) Date: 6/27/2007 Rapid Habitat: 123 American eel 2 0 2 0.2% Gen Intermediate Creek chub 7 4 11 1.2% Gen Tolerant Blacknose dace 454 252 706 80.1% Omn Tolerant Longnose dace 102 32 134 15.2% Omn Intermediate Swallowtail shiner 9 4 13 15% 1.5% Omn Tolerant Satinfin shiner 1 0 1 0.1% Inv Intermediate White sucker 5 0 5 0.6% Omn Tolerant Green sunfish 2 1 3 0.3% Gen Tolerant Tessellated darter 6 0 6 0.7% Inv Tolerant Totals 588 293 881 100.0% Algivore (Alg): 0.0% Tolerant: 84.4% Generalist (Gen): 1.8% Intermediate: 15.6% Insectivores (Ins): 0.0% Intolerant: 0.0% Invertivore (Inv): 0.8% Total: 100.0% Omnivores (Omn): 97.4% Top Predator (Top): 0.0% Filterer (Fil): 0.0% Total: 100.0% Physical Chemistry Water Temp ( C): 25.4 ph: 8.4 DO (mg/l): 12.35 DO (%): 151.4 Conductivity (uhmos): 0.630 Fish Sampling Results Site: SCSC314 Number of Species: 11 Location: Sligo Creek, downstream of Carroll Avenue Rapid Habitat: 141 Date: 8/6/08 Station is 75 meters long Species Total % Trophic Level Tolerance Level Blacknose dace 1753 83.5% Omnivore Tolerant Creek chub 28 13% 1.3% Generalist Tolerant Longnose dace 230 11.0% Omnivore Intermediate Satinfin shiner 3 0.1% Invertivore Intermediate Spotfin shiner 7 0.3% Invertivore Intermediate Swallowtail shiner 39 1.9% Omnivore Tolerant Redbreast sunfish 3 0.1% Generalist Tolerant Largemouth bass 3 0.1% Top Predator Tolerant Tessellated darter 9 0.4% Invertivore Tolerant White sucker 19 0.9% Omnivore Tolerant American eel 5 0.2% Generalist Intermediate Totals 2099 100.0% Algivores: 0.0% Tolerant: 88.3% Generalists: 1.7% Intermediate: 11.7% Insectivores: 0.0% Intolerant: 0.0% Invertivores: 0.9% Total: 100.0% Omnivores: 97.2% Top Predators: 0.1% Filterers: 0.0% Total: 100.0% 29 SCSC314 IBI DATA (2009) Fish Sampling Results Site: SCSC314 Number of Species: 12 Location: Lower Sligo Creek, access via Sligo Creek Parkway between Carroll Avenue and Jackson Avenue Rapid Habitat: 125 Date: 10/30/2009 Station is 75 meters long Anomalies Anomaly Type(s) American eel 5 0 5 0.2% Gen Intermediate Blacknose dace 1379 332 1711 82.9% Omn Tolerant 190 186 BL 4 RE Brown bullhead 0 1 1 0.0% Omn Tolerant Creek chub 7 3 10 0.5% Gen Tolerant 2 2 BL Longnose dace 141 70 211 10.2% Omn Intermediate 6 4 BL 2 RE Redbreast sunfish 3 2 5 0.2% Gen Tolerant Satinfin shiner 34 5 39 1.9% Inv Intermediate Silverjaw minnow 1 0 1 0.0% Omn Intermediate Spotfin shiner 13 4 17 0.8% Inv Intermediate Swallowtail shiner 34 2 36 1.7% Omn Tolerant Tessellated darter 20 5 25 1.2% Inv Tolerant White sucker 2 2 4 0.2% Omn Tolerant Totals 1639 426 2065 100.0% Physical Chemistry Algivore(Alg): 0.0% Tolerant: 86.8% Water Temp ( C) 13.9 Generalist(Gen): 1.0% Intermediate: 13.2% ph 7.55 Insectivores(Ins): 0.0% Intolerant: 0.0% DO 10.17 Invertivore(Inv): 3.9% Total: 100.0% DO% 97 Omnivores(Omn): 95.1% Conductivity 374 Top Predator(Top): 0.0% Filterer(Fil): 0.0% Total: 100.0% Comments: Straight channel, stabilization around sewerline @ 10 m. Extensive rip rap stabilizing both banks. Extensive invasivesalong banks. RB Sligo Creek Pkwy immediately adjacent to stream. LB Sligo Creek Trail. Sewage spill @ 50 m, not reached stream. 30 15

SCSC314 IBI DATA (2010) Fish Sampling Results Site: SCSC314 Number of Species: 13 Location: Sligo Creek, access via Sligo Creek Parkway at intersection w/ Jackson Avenue Rapid Habitat: 115 Date: 9/10/2010 Station is 75 meters long American eel 7 0 7 0.58% Generalist Intermediate Satinfin shiner 22 13 35 2.92% Invertivore Intermediate Spotfin shiner 9 5 14 1.17% Invertivore Intermediate Swallowtail shiner 31 20 51 4.25% Omnivore Tolerant Bluntnose minnow 2 0 2 0.17% Omnivore Tolerant Blacknose dace 551 130 681 56.75% Omnivore Tolerant Longnose dace 117 155 272 22.67% Omnivore Intermediate Creek chub 18 5 23 1.92% Generalist Tolerant White sucker 49 5 54 4.50% Omnivore Tolerant Brown bullhead 1 0 1 0.08% Omnivore Tolerant Redbreast sunfish 5 1 6 0.50% Generalist Tolerant Bluegill 2 0 2 0.17% Invertivore Tolerant Tessellated darter 24 28 52 4.33% Invertivore Tolerant Totals 838 362 1,200 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 72.67% Water Temp ( C) 18.9 Generalists: 3.00% Intermediate: 27.33% ph 7.76 Insectivores: 0.00% Intolerant: 0.00% DO 8.96 Invertivores: 8.58% Total: 100.00% DO% 106 Omnivores: 88.42% Conductivity 656 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% 31 SCSC314 IBI DATA (2012) Fish Sampling Results Site: SCSC314 Number of Species: 10 Location: Mainstem of Sligo Creek, access via Sligo Creek Parkway at Jackson Avenue. Rapid Habitat: 97 Date: 07/31/12 Station is 75 meters long American eel 12 3 15 1.26% Generalist Intermediate Satinfin shiner 2 2 4 0.34% Invertivore Intermediate Swallowtail shiner 22 33 55 4.62% Omnivore Tolerant Blacknose dace 357 100 457 38.40% Omnivore Tolerant Longnose dace 401 105 506 42.52% Omnivore Intermediate Creek chub 39 11 50 4.20% Generalist Tolerant White sucker 36 4 40 3.36% Omnivore Tolerant Redbreast sunfish 4 2 6 0.50% Generalist Tolerant Green sunfish 2 3 5 0.42% Generalist Tolerant Tessellated darter 43 9 52 4.37% Invertivore Tolerant Totals 918 272 1,190 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 55.88% Water Temp ( C) 22.4 Generalists: 6.39% Intermediate: 44.12% ph 7.90 Insectivores: 0.00% Intolerant: 0.00% DO 10.37 Invertivores: 4.71% Total: 100.00% DO% 119 Omnivores: 88.91% Conductivity 664 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% 32 16

NWNW421 IBI DATA (2007) Site: NWNW421 Number of species = 20 Location: Northwest Branch Date: 07/16/2007 Station is 75 meters long Rapid Habitat: Incomplete American eel 18 6 24 3.761755 Gen Intermediate Creek chub 3 0 3 0.470219 Gen Tolerant Central stoneroller 5 0 5 0.783699 Alg Intermediate Cutlips minnow 10 2 12 1.880878 Inv Intermediate Blacknose dace 11 7 18 2.821317 Omn Tolerant Longnose dace 4 1 5 0.783699 Omn Intermediate Common shiner 6 1 7 1.097179 Omn Intermediate Bluntnose minnow 215 50 265 41.53605 Omn Tolerant Spottail shiner 5 4 9 1.410658 Omn Intermediate Satinfin shiner 11 2 13 2.037618 Inv Intermediate Silverjaw minnow 15 2 17 2.664577 Omn Intermediate White sucker 136 34 170 26.64577 Omn Tolerant Northern hogsucker 0 1 1 0.15674 Inv Intolerant Margined madtom 1 0 1 0.15674 Inv Intermediate Smallmouth bass 3 0 3 0.470219 Top Intermediate Largemouth bass 2 0 2 0.31348 Top Tolerant Green sunfish 39 12 51 7.99373 Gen Tolerant Bluegill 10 2 12 1.880878 Inv Tolerant Pumpkinseed 3 2 5 0.783699 Inv Tolerant Tessellated darter 10 5 15 2.351097 Inv Tolerant Totals 507 131 638 100.0% Algivore(Alg): 0.8% Tolerant: 84.8% Generalist(Gen): 12.2% Intermediate: 15.0% Insectivores(Ins): 0.0% Intolerant: 0.2% Invertivore(Inv): 9.2% Omnivores(Omn): 77.0% Total 100.0% Top Predator(Top): 0.8% Filterer(Fil): 0.0% Total: 100.0% Water Temp ( C) 9.31 ph 6.73 DO (mg/l) 17.21 DO% 150.4 Conductivity 0.188 33 NWNW421 IBI DATA (2008) Site: NWNW421 Number of Species: 26 Location: Northwest Branch, downstream of Sunfish Hybrids: 1 route 495 and Oakview Drive Rapid Habitat: 121 Date: 9/9/08 Station is 75 meters long Species Total % Trophic Level Tolerance Level Blacknose dace 7 0.6% Omnivore Tolerant Bluntnose minnow 242 21.5% Omnivore Tolerant Common shiner 16 1.4% Omnivore Intermediate Creek chub 1 0.1% Generalist Tolerant Cutlips minnow 40 3.6% Invertivore Intermediate E. silvery minnow 5 0.4% Algivore Intermediate Longnose dace 8 0.7% Omnivore Intermediate Satinfin shiner 5 0.4% Invertivore Intermediate Silverjaw minnow 9 0.8% Omnivore Intermediate Spotfin shiner 37 3.3% Invertivore Intermediate Spottail shiner 128 11.4% Omnivore Intermediate Swallowtail shiner 46 4.1% Omnivore Tolerant Bluegill 15 1.3% Invertivore Tolerant Green sunfish 4 0.4% Generalist Tolerant Pumpkinseed 52 4.6% Invertivore Tolerant Pumpkinseed Redbreast sunfish hybrid 1 0.1% Generalist Tolerant Redbreast sunfish 125 11.1% Generalist Tolerant Largemouth bass 4 0.4% Top Predator Tolerant Smallmouth bass 4 0.4% Top Predator Intermediate Tessellated darter 50 4.5% Invertivore Tolerant Margined madtom 5 0.4% Invertivore Intermediate Creek chubsucker 1 0.1% Omnivore Intermediate Northern hogsucker 8 0.7% Invertivore Intolerant White sucker 228 20.3% Omnivore Tolerant American eel 64 5.7% Generalist Intermediate Banded Killifish 6 0.5% Invertivore Intermediate Sea Lamprey 12 1.1% Filterer Intermediate Totals 1123 100.0% Fish Sampling Results Site: NWNW421 Number of Species: 26 Location: Northwest Branch, downstream of Sunfish Hybrids: 1 route 495 and Oakview Drive Rapid Habitat: 121 Date: 9/9/08 Station is 75 meters long 34 Algivores: 0.4% Tolerant: 69.0% Generalists: 17.4% Intermediate: 30.3% 17

NWNW421 IBI DATA (2009) Site: NWNW421 Number of Species: 17 Location: Lower Mainstem of NW Branch, access via Oakview Drive from New Hampshire Avenue Rapid Habitat: 136 Date: 8/26/2009 Station is 75 meters long American eel 57 16 73 21.4% Gen Intermediate Bluegill 7 7 14 4.1% Inv Tolerant Creek chub 0 4 4 1.2% Gen Tolerant Cutlips minnow 8 1 9 26% 2.6% Inv Intermediate t Fantail darter 1 0 1 0.3% Ins Intermediate Hybrid sunfish 1 0 1 0.3% Gen Tolerant Largemouth bass 3 4 7 2.1% Top Tolerant Longnose dace 2 0 2 0.6% Omn Intermediate Margined madtom 3 0 3 0.9% Inv Intermediate Northern hogsucker 7 3 10 2.9% Inv Intolerant Pumpkinseed 32 16 48 14.1% Inv Tolerant Redbreast sunfish 68 37 105 30.8% Gen Tolerant Sea Lamprey 3 0 3 0.9% Fil Intermediate Smallmouth bass 3 1 4 1.2% Top Intermediate Tessellated darter 7 2 9 2.6% Inv Tolerant White sucker 98 22 120 35.2% Omn Tolerant Yellow bullhead 1 0 1 0.3% Omn Intermediate Totals 301 113 341 121.4% Physical Chemistry Algivore(Alg): 0.0% Tolerant: 90.3% Water Temp ( C) 22.3 Generalist(Gen): 53.7% Intermediate: 28.2% ph 7.31 Insectivores(Ins): 0.3% Intolerant: 2.9% DO 9.61 Invertivore(Inv): 27.3% Total: 121.4% DO% 113 Omnivores(Omn): 36.1% Conductivity 237 Top Predator(Top): 3.2% Filterer(Fil): 0.9% Total: 121.4% 35 Biological Monitoring Data: 2012 Fish Site: NWNW421 Number of Species: 12 Location: Mainstem Northwest Branch of the Anacostia River, access via Oakview Drive. Rapid Habitat: 94 Date: 08/16/12 Station is 75 meters long Sea Lamprey 0 1 1 0.66% Filterer Intermediate American eel 26 18 44 28.95% Generalist Intermediate Cutlips minnow 6 1 7 4.61% Invertivore Intermediate White sucker 4 6 10 6.58% Omnivore Tolerant Northern hogsucker 17 5 22 14.47% Invertivore Intolerant Margined madtom 0 2 2 1.32% Invertivore Intermediate Redbreast sunfish 23 13 36 23.68% Generalist Tolerant Pumpkinseed 4 4 8 5.26% Invertivore Tolerant Bluegill 4 4 8 5.26% Invertivore Tolerant Smallmouth bass 3 3 6 3.95% Top Predator Intermediate Largemouth bass 0 2 2 1.32% Top Predator Tolerant Tessellated darter 4 2 6 3.95% Invertivore Tolerant Totals 91 61 152 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 46.05% Water Temp ( C) 20.4 Generalists: 52.63% Intermediate: 39.47% ph 7.53 Insectivores: 0.00% Intolerant: 14.47% DO 8.27 Invertivores: 34.87% Total: 100.00% DO% 92 Omnivores: 6.58% Conductivity 234 Top Predators: 5.26% Filterers: 0.66% Total: 100.00% IBI: 28 (Fair) 36 18

2012 Raw Data 37 Biological Monitoring Data: 2012 Fish Site: PBUT201 Number of Species: 0 Location: Unnamed tributary of Paint Branch Creek, access via East Randolf Road Rapid Habitat: 101 Date: 07/12/12 Station is 75 meters long Totals 0 0 0 0.00% Physical Chemistry Algivores: 0.00% Tolerant: 0.00% Water Temp ( C) 19.5 Generalists: 0.00% Intermediate: 0.00% ph 6.19 Insectivores: 0.00% Intolerant: 0.00% DO 7.01 Invertivores: 0.00% Total: 0.00% DO% 76 Omnivores: 0.00% Conductivity 220 Top Predators: 0.00% Filterers: 0.00% Total: 0.00% IBI: 0 (Poor) 38 19

Biological Monitoring Data: 2012 Fish Site: NWND101 Number of Species: 8 Location: Northwood tributary of Northwest Branch Middle Mainstem, access via Loxford Terrace. Rapid Habitat: 118 Date: 08/08/12 Station is 75 meters long Common shiner 1 1 0.47% Invertivore Intermediate Bluntnose minnow 6 1 7 3.29% Omnivore Tolerant Blacknose dace 134 37 171 80.28% Omnivore Tolerant Longnose dace 4 1 5 2.35% Omnivore Intermediate Creek chub 16 3 19 8.92% Generalist Tolerant White sucker 2 2 0.94% Omnivore Tolerant Redbreast sunfish 5 2 7 3.29% Generalist Tolerant Pumpkinseed 1 1 0.47% Invertivore Tolerant Totals 169 44 213 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 97.18% Wt Water Temp ( C) 20.9 Generalists: 12.21% 21% Intermediate: t 2.82% ph 6.76 Insectivores: 0.00% Intolerant: 0.00% DO 3.94 Invertivores: 0.94% Total: 100.00% DO% 41 Omnivores: 86.85% Conductivity 216 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% IBI: 22 (Fair) 39 Biological Monitoring Data: 2012 Fish Site: NWNW413 Number of Species: 22 Location: Middle Mainstem Northwest Branch of the Anacostia River, access via Lamberton Drive. Rapid Habitat: 117 Date: 08/29/12 Station is 75 meters long Satinfin shiner 14 4 18 0.79% Invertivore Intermediate Cutlips minnow 14 9 23 1.01% Invertivore Intermediate Common shiner 2 12 14 0.61% Invertivore Intermediate Silverjaw minnow 12 22 34 1.49% Omnivore Intermediate Spottail shiner 21 26 47 2.06% Omnivore Intermediate Swallowtail shiner 59 58 117 5.12% Omnivore Tolerant Bluntnose minnow 594 870 1,464 64.10% Omnivore Tolerant Blacknose dace 61 80 141 6.17% Omnivore Tolerant Longnose dace 75 68 143 6.26% Omnivore Intermediate Creek chub 3 1 4 0.18% Generalist Tolerant White sucker 57 22 79 3.46% Omnivore Tolerant Northern hogsucker 2 1 3 0.13% Invertivore Intolerant Margined madtom 4 3 7 0.31% Invertivore Intermediate E. Mosquitofish 4 2 6 0.26% Invertivore Tolerant Redbreast sunfish 42 28 70 3.06% Generalist Tolerant Green sunfish 0 1 1 0.04% Generalist Tolerant Pumpkinseed 3 0 3 0.13% Invertivore Tolerant Bluegill 1 6 7 0.31% Invertivore Tolerant Smallmouth bass 1 0 1 0.04% Top Predator Intermediate Largemouth bass 2 3 5 0.22% Top Predator Tolerant Fantail darter 12 18 30 1.31% Insectivore Intermediate Tessellated darter 36 31 67 2.93% Invertivore Tolerant Totals 1,019 1,265 2,284 100.00% 40 20

Biological Monitoring Data: 2012 Fish Site: NWNW421 Number of Species: 12 Location: Mainstem Northwest Branch of the Anacostia River, access via Oakview Drive. Rapid Habitat: 94 Date: 08/16/12 Station is 75 meters long Sea Lamprey 0 1 1 0.66% Filterer Intermediate American eel 26 18 44 28.95% Generalist Intermediate Cutlips minnow 6 1 7 4.61% Invertivore Intermediate White sucker 4 6 10 6.58% Omnivore Tolerant Northern hogsucker 17 5 22 14.47% Invertivore Intolerant Margined madtom 0 2 2 1.32% Invertivore Intermediate Redbreast sunfish 23 13 36 23.68% Generalist Tolerant Pumpkinseed 4 4 8 5.26% Invertivore Tolerant Bluegill 4 4 8 5.26% Invertivore Tolerant Smallmouth bass 3 3 6 3.95% Top Predator Intermediate Largemouth bass 0 2 2 1.32% Top Predator Tolerant Tessellated darter 4 2 6 3.95% Invertivore Tolerant Totals 91 61 152 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 46.05% Water Temp ( C) 20.4 Generalists: 52.63% Intermediate: 39.47% ph 7.53 Insectivores: 0.00% Intolerant: 14.47% DO 8.27 Invertivores: 34.87% Total: 100.00% DO% 92 Omnivores: 6.58% Conductivity 234 Top Predators: 5.26% Filterers: 0.66% Total: 100.00% IBI: 28 (Fair) 41 Biological Monitoring Data: 2012 Fish Site: SCSC314 Number of Species: 10 Location: Mainstem of Sligo Creek, access via Sligo Creek Parkway at Jackson Avenue. Rapid Habitat: 97 Date: 07/31/12 Station is 75 meters long American eel 12 3 15 1.26% Generalist Intermediate Satinfin shiner 2 2 4 0.34% Invertivore Intermediate Swallowtail shiner 22 33 55 4.62% Omnivore Tolerant Blacknose dace 357 100 457 38.40% Omnivore Tolerant Longnose dace 401 105 506 42.52% Omnivore Intermediate Creek chub 39 11 50 4.20% Generalist Tolerant White sucker 36 4 40 3.36% Omnivore Tolerant Redbreast sunfish 4 2 6 0.50% Generalist Tolerant Green sunfish 2 3 5 0.42% Generalist Tolerant Tessellated darter 43 9 52 4.37% Invertivore Tolerant Totals 918 272 1,190 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 55.88% Water Temp ( C) 22.4 Generalists: 6.39% Intermediate: 44.12% ph 7.90 Insectivores: 0.00% Intolerant: 0.00% DO 10.37 Invertivores: 4.71% Total: 100.00% 00% DO% 119 Omnivores: 88.91% Conductivity 664 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% IBI: 28 (Fair) 42 21

2011 Raw Data 43 Biological Monitoring Data: 2011 Fish Site: NWLR102 Number of Species: 2 Location: Lockridge Drive tributary of Northwest Branch, access via Lockridge Drive. Rapid Habitat: 97 Date: 06/09/11 Station is 75 meters long Blacknose dace 2 1 3 42.86% Omnivore Tolerant Creek chub 3 1 4 57.14% Generalist Tolerant Totals 5 2 7 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 100.00% Water Temp ( C) 20.6 Generalists: 57.14% Intermediate: 0.00% ph 7.17 Insectivores: 0.00% Intolerant: 0.00% DO 5.92 Invertivores: 0.00% Total: 100.00% DO% 66 Omnivores: 42.86% Conductivity 0.6 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% IBI: 14 (Poor) 44 22

2010 Raw Data 45 Biological Monitoring Data: 2010 Fish Site: PBPB311 Number of Species: 14 Location: Paint Branch Mainstem, access via GSA property in White Oak Rapid Habitat: 127 Date: 9/29/2010 Station is 75 meters long Sea Lamprey 1 0 1 0.13% Filterer Intermediate American eel 14 3 17 2.28% Generalist Intermediate Rosyside dace 52 18 70 9.37% Invertivore Intermediate Cutlips minnow 11 3 14 1.87% Invertivore Intermediate Common shiner 2 0 2 0.27% Invertivore Intermediate Blacknose dace 222 103 325 43.51% Omnivore Tolerant Longnose dace 64 36 100 13.39% Omnivore Intermediate Creek chub 25 10 35 4.69% Generalist Tolerant Fallfish 10 3 13 1.74% Generalist Intermediate White sucker 32 17 49 6.56% Omnivore Tolerant Northern hogsucker 34 29 63 8.43% Invertivore Intolerant Margined madtom 20 5 25 3.35% Invertivore Intermediate Blue Ridge sculpin 14 12 26 3.48% Insectivore Intolerant Tessellated darter 6 1 7 0.94% Invertivore Tolerant Totals 507 240 747 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 55.69% Water Temp ( C) 18.0 Generalists: 8.70% Intermediate: 32.40% ph 6.71 Insectivores: 3.48% Intolerant: 11.91% DO 9.23 Invertivores: 24.23% Total: 100.00% DO% 98 Omnivores: 63.45% Conductivity 191 Top Predators: 0.00% Filterers: 0.13% Total: 100.00% 46 IBI: 30 (Fair) 23

Biological Monitoring Data: 2010 Fish Site: PBWF102 Number of Species: 6 Location: West Farm Tributary to Paint Branch, access via GSA property in White Oak Rapid Habitat: 154 Date: 9/29/2010 Station is 75 meters long American eel 1 0 1 0.75% Generalist Intermediate Rosyside dace 19 6 25 18.66% Invertivore Intermediate Blacknose dace 63 17 80 59.70% Omnivore Tolerant Longnose dace 1 1 2 1.49% Omnivore Intermediate Creek chub 15 10 25 18.66% Generalist Tolerant White sucker 1 0 1 0.75% Omnivore Tolerant Totals 100 34 134 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 79.10% Water Temp ( C) 18.4 Generalists: 19.40% Intermediate: 20.90% ph 7.61 Insectivores: 0.00% Intolerant: 0.00% DO 8.42 Invertivores: 18.66% Total: 100.00% DO% 90 Omnivores: 61.94% Conductivity 546 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% IBI: 22 (Fair) 47 Biological Monitoring Data: 2010 Fish Site: SCFT102a Number of Species: 1 Location: Flora Lane Tributary of Sligo Creek, access via Crosby Road from Dale Drive Rapid Habitat: 82 Date: 8/30/2010 Station is approximately 65 meters long (site spans from middle of Flora Lane trib up to weir) Blacknose dace 5 1 6 100.00% Omnivore Tolerant Totals 5 1 6 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 100.00% Water Temp ( C) 20.7 Generalists: 0.00% Intermediate: 0.00% ph 7.05 Insectivores: 0.00% Intolerant: 0.00% DO 5.68 Invertivores: 0.00% Total: 100.00% DO% 64 Omnivores: 100.00% Conductivity 628 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% IBI: 14 (Poor) 48 24

Biological Monitoring Data: 2010 Fish Site: SCFT102b Number of Species: 6 Location: Flora Lane Tributary of Sligo Creek, access via Crosby Road from Dale Drive Rapid Habitat: 95 Date: 8/30/2010 Station is 75 meters long (site spans from confluence w/ Sligo mainstem up to culvert / bridge) Rosyside dace 1 0 1 0.25% Invertivore Intermediate Blacknose dace 219 125 344 85.57% Omnivore Tolerant Creek chub 18 9 27 6.72% Generalist Tolerant White sucker 14 4 18 4.48% Omnivore Tolerant Green sunfish 3 0 3 0.75% Generalist Tolerant Tessellated darter 6 3 9 2.24% Invertivore Tolerant Totals 261 141 402 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 99.75% Water Temp ( C) 21.2 Generalists: 7.46% Intermediate: 0.25% ph 7.31 Insectivores: 0.00% Intolerant: 0.00% DO 8.18 Invertivores: 2.49% Total: 100.00% DO% 92 Omnivores: 90.05% IBI: 20 (Poor) Conductivity 622 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% 49 Biological Monitoring Data: 2010 Fish Site: SCSC314 Number of Species: 13 Location: Sligo Creek, access via Sligo Creek Parkway at intersection w/ Jackson Avenue Rapid Habitat: 115 Date: 9/10/2010 Station is 75 meters long American eel 7 0 7 0.58% Generalist Intermediate Satinfin shiner 22 13 35 2.92% Invertivore Intermediate Spotfin shiner 9 5 14 1.17% Invertivore Intermediate Swallowtail shiner 31 20 51 4.25% Omnivore Tolerant Bluntnose minnow 2 0 2 0.17% Omnivore Tolerant Blacknose dace 551 130 681 56.75% Omnivore Tolerant Longnose dace 117 155 272 22.67% Omnivore Intermediate Creek chub 18 5 23 1.92% Generalist Tolerant White sucker 49 5 54 4.50% Omnivore Tolerant Brown bullhead 1 0 1 0.08% Omnivore Tolerant Redbreast sunfish 5 1 6 0.50% Generalist Tolerant Bluegill 2 0 2 0.17% Invertivore Tolerant Tessellated darter 24 28 52 4.33% Invertivore Tolerant Totals 838 362 1,200 100.00% Physical Chemistry Algivores: 0.00% Tolerant: 72.67% Water Temp ( C) 18.9 Generalists: 3.00% Intermediate: 27.33% ph 7.76 Insectivores: 0.00% Intolerant: 0.00% DO 8.96 Invertivores: 8.58% Total: 100.00% DO% 106 Omnivores: 88.42% Conductivity 656 Top Predators: 0.00% Filterers: 0.00% Total: 100.00% IBI: 28 (Fair) 50 25