Validation of a Step Test in Children Ages 7-11

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Validation of a Step Test in Children Ages 7-11 Emilene Clark Aurora Fry Colleen Smith Tonya Thomas Primary Advisor: Jim Farris, PT, PhD April 20, 2011

Background Exercise Testing Maximal Testing Sub-maximal testing Modified step test protocol

Research Question 1. Can a submaximal step test be developed to accurately predict maximal aerobic capacity in children? 2. What are the best predictors of maximal aerobic capacity? 1. Anthropometric measurements 2. Heart rate response 3. Step height

Purpose The purpose of this study is to design a portable and reproducible, validated step test that predicts peak aerobic capacity in healthy children ages 7-11 years old, that can be administered by health care professionals, educators, and caregivers in a variety of settings.

Hypothesis By creating a submaximal step test that will adjust to the height of each child, and measuring their heart rate at numerous times, we believe, an accurate prediction of aerobic capacity can be obtained.

Methods Subjects Anthropometrics Sub-maximal Test Peak Aerobic Capacity

Subjects 128 male and female children Inclusion Criteria Healthy male and female children, ages 7-11 Exclusion Criteria Diagnosed disorders made worse with exercise Non-English speakers without interpreter present during all components of the study Recruiting A.T. Still University, YMCA, Community

Anthropometric Measurements Height and weight Blood pressure Resting & Recovery Body circumferences Skin fold

Sub-maximal Test 2, 3-minute step tests Instruction provided Step height calculated Resting HR established Metronome set at 104 beats/min HR taken immediately following completion, 15 & 30 seconds, 1,2 & 3 minutes Second test with alternate step height

Step Height (inches) Based on Child s Height Step Height Gender Age: 7 8 9 10 11 8 F <40.74 <40.74 <40.12 <39.81 <39.36 M <40.90 <40.90 <40.58 <39.96 <39.51 10 F 40.74-51.62 40.74-51.62 40.12-50.83 39.81-50.44 39.36-49.88 M 40.90-51.82 40.90-51.82 40.58-50.42 39.96-50.63 39.51-50.06 12 F 51.62-62.49 51.62-62.49 50.83-61.07 50.44-60.39 49.88-60.39 M 51.82-62.74 51.82-62.74 50.42-61.25 50.63-61.30 50.06-60.61 14 F 62.49-73.36 62.49-73.36 61.07-72.25 60.39-71.70 60.39-70.90 M 62.74-73.66 62.74-73.09 61.25-73.09 61.30-71.98 60.61-71.16

Peak Aerobic Capacity Continuous, incremental, walking and running protocol on a treadmill was used. Running speed was adapted to each subject through 3-5 minute familiarization session.

Peak Aerobic Capacity Each stage was 2 minutes in duration Stage 1: 2.5 mph, 0% grade Stage 2: x.x jogging speed, 0% grade Stage 3: x.x jogging speed, 6% grade Stage 4: x.x + 0.3 mph, 6% grade Stage 5: x.x + 0.6 mph, 6% grade Stage 6: x.x + 0.9 mph, 6% grade If fatigue is not accomplished by minute 12, grade was increased 2% every 2 minutes

Statistical Analysis Pearson s Correlation Coefficient (r) Anthropometric measurements HR responses at all time points and step heights Peak aerobic capacity Stepwise Regression ANOVA determined if regression model was significant or not

Results Subject Characteristics Blood pressure and BMI Anthropometric Measurements HR response to Step Test VO2 by age x gender Predictive Models Development of the Models

Subject Characteristics Age, yr 7 8 9 10 11 Female (n) 7 10 9 5 7 Male (n) 5 8 11 8 5 Height, cm F: 49.68 M: 48.90 Body Mass, kg F: 27.72 M: 26.36 BMI, kg/m 2 F: 17.59 M: 17.40 SBP, mmhg F: 108.57 M: 93.20 DBP, mmhg F: 76.29 M: 69.20 F: 50.68 M: 50.09 F: 27.27 M: 25.00 F: 16.50 M: 15.76 F: 104.40 M: 107.13 F: 68.20 M: 71.00 F: 53.69 M: 53.61 F: 36.15 M: 37.34 F: 19.12 M: 19.82 F: 114.00 M: 115.09 F: 76.44 M: 74.27 F: 56.35 M: 56.59 F: 38.05 M: 38.25 F: 18.56 M: 18.34 F: 108.40 M: 111.63 F: 69.60 M: 74.38 F: 60.59 M: 57.20 F: 47.57 M: 36.01 F: 19.94 M: 17.06 F: 120.86 M: 113.60 F: 73.43 M: 70.80

Anthropometrics Circumference Measurements (cm) Skinfolds (mm) HR Age Sex Arm Forearm Waist Abdomen Buttocks Hips Mid-thigh Calf Triceps Calf BPM (Resting) 7 8 9 10 11 Female 20.29 19.38 22.44 61.89 72.49 66.95 35.58 27.04 17.95 19.19 90.95 Male 19.84 19.00 58.17 59.82 65.74 64.86 33.69 24.44 18.20 17.53 83.02 Female 19.40 18.40 55.27 60.41 68.39 65.18 33.08 25.98 16.33 16.66 88.20 Male 18.02 18.06 55.54 58.03 66.06 63.00 35.96 25.33 12.24 13.32 86.25 Female 23.67 19.35 63.36 69.17 77.91 74.13 37.98 30.71 21.30 19.52 86.25 Male 22.56 20.70 65.03 68.58 76.93 73.02 39.96 29.56 25.95 30.12 95.27 Female 22.60 21.04 62.22 66.79 77.13 74.31 39.08 30.16 19.80 19.20 89.80 Male 22.06 26.71 63.43 65.72 77.06 75.82 38.86 29.42 21.25 22.50 83.88 Female 24.39 22.26 68.96 76.45 87.31 84.86 42.83 32.81 17.95 20.05 92.71 Male 21.32 20.33 61.90 64.03 77.69 73.22 38.36 28.83 14.80 14.94 79.00

Heart Rate Response to Step Test Time Recorded Average High HR Resting HR 87.24 + 10.69, 1.24 Immediate 157.24+ 16.22, 1.87 15 sec 147.77+ 16.34, 1.89 1 min 102.16+ 19.88, 2.30 2 min 94.00+ 15.85, 1.83 3 min 95.17+ 14.21, 1.64

Development of Predictive Models Correlation analysis and ANOVA Direct Entry Regression Age, and Gender Stepwise Entries All Heart Rate Time Points & Step Heights HR at Immediate Post Exercise, High step All Anthropometric Data BMI Category AND Body Fat

Development of Predictive Models Peak VO2 Correlated to HR Response in Low Step and High Step Conditions Low step High step Immediately -0.645-0.685 15 seconds -0.584-0.663 1 minute -0.493-0.492 2 minute -0.453-0.424 3 minute -0.413-0.407 Rest -0.402-0.387

Development of Predictive Models Correlations of BMI to Body Fat Percentage Chart Anthropometric Measures Body Fat Percentage (from skin folds) BMI Arm 0.709 0.913 Forearm 0.203 0.328 Waist, cm 0.653 0.882 Abdomen 0.686 0.893 Buttocks_hips 0.626 0.838 Hips_thigh 0.565 0.794 Midthigh 0.483 0.730 Calf 0.588 0.782 Circumference total 0.745 0.917 Body fat percentage ------ 0.809

VO2 (ml/kg/min) Gender Peak VO2 (ml/kg/min) 50 45 40 35 30 25 20 42.511 Female Male 48.191

Peak VO2 By BMI Category Peak VO2 60 50 40 30 20 10 *Significantly different from Underweight and Normal * * 0 Underweight Normal Overweight Obese Correlation for BMI Category & VO2: R = 0.605, p<0.01

Predictive Models Predictive Model 1 VO2ml/kg = 69.324 (HR Immed_H * 0.178) + (Age * 0.582) + (Gender * 3.553) (BMI_Category * 2.388) (Bodfat * 0.130) R 2 : 0.632 Predictive Model 2 VO2ml/kg = 71.439 (HR Immed_H * 0.198) + (Age * 0.58) + (Gender * 2.935) (BMI_Category * 3.727) R 2 : 0.610 Gender: 1=female, 2= male

Discussion Validation of predictive model Comparison to other literature.

Conclusion In conclusion, a submaximal step test was designed and validated that predicted peak aerobic capacity in healthy children ages 7-11 years old that can be administered by health care professionals, educators, and caregivers in a variety of settings.

Next Steps Next Step Perform step tests in community Create national norms / percentile ranks

References

We thought you may ask that Predictive Model 1-15 sec: VO2ml/kg = 66.287 (15 sec_h * 0.170) + (Age * 0.678) + (Gender * 3.430) (BMI_Category * 2.504) (Bodfat * 0.139) R 2 : 0.627 Predictive Model 1-60 sec: VO2ml/kg = 49.964 (1 min_h * 0.054) + (Age * 0.331) + (Gender * 4.719) (BMI_Category * 2.845) (Bodfat * 0.198) R 2 : 0.548

Predictive Model 2-15sec: VO2ml/kg = 67.980 (15 sec_h * 0.190) + (Age * 0.684) + (Gender * 2.767) (BMI_Category * 3.975) R 2 : 0.601 Predictive Model 2-60 sec: VO2ml/kg = 48.672 (1 min_h * 0.053) + (Age * 0.276) + (Gender * 4.071) (BMI_Category * 5.247) R 2 : 0.494