The Design of In vivo Conservation Programmes In any population it is inevitable that some genetic variation will be lost over time
Minimisation of the loss of genetic variation is equivalent to minimisation of the rate of inbreeding in a population The rate of increase of inbreeding (ΔF) is the most important parameter in programmes that maintain genetic diversity The rate of inbreeding is more important than the actual level of inbreeding, because the actual level of inbreeding is relative to some base population, which is assumed unrelated and non-inbred The inbreeding coefficient (F) is not a suitable parameter to describe a small population
The genetic size of a population is most easily described by the effective population size (N e ) which is a number that is generally smaller than the census size of the population. The effective size is determined by the rate of inbreeding (and vice versa): DF = 1/(2N e ). The target is to have an effective population size is 50 animals per generation or more. The effective size of 50 corresponds to a rate of inbreeding of 1% per generation. All conserved populations should have achieving this minimum effective size as their first objective
The generation interval is the genetic unit of time for populations
Most practical breeding schemes involve overlapping generations where there are parents of different ages being used. The average relationship of a population The average relationship in a group of individuals equals: one quarter the average relationship among the males + one quarter the average relationship among the females + one half the average relationship between the males and the females If we calculate the relationship between every possible sire and dam, we can choose the sire and dam pairs that yield the offspring with the lowest inbreeding coefficients
What kind of problems may be present at the start? Bottle necks the conservation programme will try to conserve the remaining genetic diversity as much as possible Populations in genetic melt-down A population that is in a genetic melt-down is one which is not fit enough to reproduce itself and thus the number of animals are irrevocably decreasing every generation, i.e. the population is heading for extinction It can be conserved in vivo by: limited crossing with another breed that is fitted to a similar environment changing the environment of the animals, so that their fitness increases and natural selection can further increase its fitness cryoconservation
How should conserved populations be maintained and improved? The desired effective population size is about 50 animals per generation.
A prolonged generation interval can increase the effective population size In live animals the generation interval is limited by the maximum age of the animals, but if we can freeze the embryos, the generation interval can be much prolonged Selection of sires and dams The recommended rule for the initial stages of a conservation programme is that selection should be within families, i.e. a sire is replaced by one of his sons, and a dam is replaced by one of her daughters. This minimises the rate of inbreeding. The rule that a sire is replaced by one of his sons and a dam by one of her daughters, leaves some room for selection.
When mating the selected sires and dams, the mating of sires and dams that are full-sibs should be avoided, and where possible half-sibs. Complete avoidance of other relatives is impossible after the initial generations. A conservation programme is most likely to maintain its funding when there is some commercial use of the animals. Examples are the production and marketing of special cheeses, or better adaptation to local environments. When a niche has been identified, a breeding scheme has to be set up to achieve the niche: 1. The breeding goal should be derived 2. A selection index has to be set up 3. The selection should be performed
What role do nucleus populations play in conservation programmes? It is often desirable to identify a subset of the population upon which to concentrate limited resources. This will be called the nucleus population. There are issues to address: 1. Size Ne >= 50 2. establishment 3. location 4. management 2. the average relationships among the sires and dams chosen to produce the first managed generation of the nucleus should be minimised. The unrelated animals will be more representative of the wider population.
3. The conserved population could be housed in a central nucleus herd or in several dispersed herds. It is recommended to choose the dispersed herds option, because a central nucleus herd could be wiped out by diseases, fires, and other natural disasters. Some agreed uniformity of management is desirable. With a central nucleus risk to in-situ schemes from genotype by environment interactions (in which genotypes perform differently in different environments) since the population may adapt over time to the local environment of the nucleus. With a dispersed nucleus risk to in-situ schemes is that the dispersal extends beyond the environment of origin A further consideration on dispersal is the very practical question of the location of those farmers interested in keeping the breed.
Genotype by environment interactions favour the keeping of the animals in an environment that resembles their natural environment as closely as possible With the animals being kept at dispersed herds, we must avoid the occurrence of small distinct populations in each of the herds with no connections between the herds. Setting up a rotational breeding scheme Recording One of the drawbacks of conservation programmes with a dispersed nucleus is that the quality of the recording of the pedigree and traits may be poor in some or all of these herds. Record at least the sire and the dam of each animal This requires identification of the animal The recording should enter a databank
Coping without pedigree recording In these situations we should try to estimate the number of sires and dams that are used per generation. The census or survey should ask questions such as: 1. how many sires does the farmer use per season; 2. from where does the farmer obtain sires (there may be a small breeding nuclei somewhere in the population, so many sires might be related); 3. how many dams does the farmer use per sire (the number of dams per sire times the number of sires yields the total number of dams); 4. the litter size of the breed; 5. selection of replacement dams.
Using this information it may be possible to establish the links between groups of keepers, and whether or not a hierarchical population structure exists within the breed. Once this information is collected then, together with survey information on breeding ages, allows estimation of the inbreeding rate per generation. If the estimated rate of inbreeding is more than 1%, the conservation plan should try to get the farmers to use more sires. Until this information becomes available, then the breeding scheme should ensure that wherever possible a minimum of 6 villages take part in the conservation scheme and that a system for rotating the breeding males among these villages is adopted.