Supplement of Biogeosciences, 15, 3691 371, 21 https://doi.org/1.519/bg-15-3691-21-supplement Author(s) 21. This work is distributed under the Cretive Commons Attribution. License. Supplement of Popultion-specific responses in physiologicl rtes of Emilini huxleyi to brod CO 2 rnge Yong Zhng et l. Correspondence to: Yong Zhng (zhngyong193@xmu.edu.cn) The copyright of individul prts of the supplement might differ from the CC BY. License.
Tble S1. Se surfce temperture (SST) t the Azores, Bergen nd Cnry Islnds. Men monthly Min. monthly Mx. monthly SST rnge ( o C) SST ( o C) SST ( o C) References Azores 15.6 22.3.6 32.9 Wisshk et l., 21 Bergen 6.. 2.6 Smuelsen, 197 Locrnini et l., 26 Cnry Islnds 1. 23.5 17.5 2. Sntn-Csino et l., 27
Tble S2. Number of bse pirs (bp) in the exmined loci (mrked with sterisk) in the lleles of individul genotypes. The numbers of bse pirs in ll exmined loci re different, which indictes different genotypes within popultion. NA indictes tht no lleles could be obtined. Genotype P2E9 * P2B * P2F11 * EHMS37 * EHMS15 * A23 97 97 27 27 11 15 21 23 26 21 A22 99 99 29 211 13 15 197 197 26 26 A21 97 11 27 29 11 137 269 273 26 21 A19 99 11 27 27 13 131 273 23 26 26 A13 95 97 213 223 7 9 297 31 26 26 A1 99 11 27 27 7 7 21 273 26 26 B95 95 99 29 29 11 11 21 25 26 21 B63 97 97 27 27 11 13 23 29 26 21 B62 97 99 27 27 11 13 25 211 26 21 B51 95 97 27 27 11 13 25 25 26 21 B1 97 11 27 27 11 11 21 211 26 21 B17 97 11 27 27 11 13 25 25 NA NA C9 1 29 211 111 135 233 27 95 95 C91 6 12 219 219 131 11 27 29 9 135 C9 1 26 26 5 135 NA NA 139 19 C1 1 26 26 5 135 NA NA 139 19 C35 96 96 215 219 131 131 25 25 NA NA
Tble S3. Clculted optimum, mximum vlue (V mx ) nd reltive sensitivity (rs, ) of POC nd PIC quots of ech E. huxleyi strin. POC quot PIC quot optimum V mx (pg rs optimum V mx (pg rs Strin (µtm) C cell 1 ) (µtm) C cell 1 ) A23 7 1.75.3 266 11.33.25 A22 657 1..23 76 1.17. A21 73 13.5.35 36.92.9 A19 6 13.3.1 52 15.1.59 A13 6 9.69.52 55 11.9.53 A1 56 11.22.6 35 9.2.17 B95 925 1.6.1 59 7.33.25 B63 715 13.39.17 63 1.3.36 B62 39 1.6.5 6 1.96.35 B51 635 13.1.3 7 9.2.39 B1 93 1.73.3 517 7.62.29 B17 11.69.31 635 9..37 C9 65.7.6 59 6.3.2 C91 1 5.2.69 1 11.97.3 C9 6.76.2 2.9.6 C1 675 7.71.37 623 11..2 C35 72.9.39 53.7.26
the Azores strins A23, A22, A21, A19, A13, A1 the Bergen strins B95, B63, B62, B51, B1, B17 the Cnry Islnds strins C9, C91, C9, C1, C35 1 2 3 5 6 A23 A22 A21 A19 A13 A1 B95 B63 B62 B51 B1 B17 C9 C91 C9 C1 C35 Ech strin ws incubted for to 6 dys t 11 CO 2 levels rnging from 115 to 37 μtm with no replicte At the end of the incubtions, growth, POC nd PIC production rtes were mesured Figure S1. A flow chrt for the experimentl processes.
PIC quot (pg C cell 1 ) POC quot (pg C cell 1 ) reltive PIC quot (%) reltive POC quot (%) () 1 1 (c) Azores Bergen Cnry Islnds 6 2 (b) 1 1 (d) 6 2 ( tm) ( tm) Figure S2. Optimum curve responses of POC nd PIC quots of three E. huxleyi popultions to rnge from µtm to 263 µtm. Responses of POC quot () nd PIC quot (b) to. Responses of reltive POC quot (c) nd reltive PIC quot (d) to. For more detil informtion, see Figure 1.
optimum of PIC quot ( tm) mximum PIC quot (pg C cell 1 ) reltive sensitivity of PIC quot ( ) optimum of POC quot ( tm) mximum POC quot (pg C cell 1 ) reltive sensitivity of POC quot ( ) 1 15 9 6 clculted optimum () 15 9 6 clculted mximum vlue (c) b..6..2 fitted reltive sensitivity b (e) b 3 Azores Bergen Cnry Islnds 3 Azores Bergen Cnry Islnds. Azores Bergen Cnry Islnds 75 6 5 (b) (d).9.6 (f) 3.3 15 Azores Bergen Cnry Islnds Azores Bergen Cnry Islnds. Azores Bergen Cnry Islnds Figure S3. Clculted optimum, clculted mximum vlue nd fitted reltive sensitivity constnt of POC nd PIC quots of ech popultion. () optimum of POC quot; (b) optimum of PIC quot; (c) mximum POC quot; (d) mximum PIC quot; (e) reltive sensitivity constnt of POC quot; (f) reltive sensitivity constnt of PIC quot. For more detil informtion, see Figure 2.
PIC quot (pg C cell 1 ) POC quot (pg C cell 1 ) Azores Bergen Cnry Islnds A23 A22 A21 A19 A13 A1 () B95 B63 B62 B51 B1 B17 (b) (c) C9 C91 C9 C1 C35 (d) (e) (f) ( tm) ( tm) ( tm) Figure S. Optimum curve responses of POC nd PIC quots of individul E. huxleyi strins in the Azores (left), Bergen (medium) nd Cnry Islnds (right) popultions to CO 2 rnge from 115 µtm to 37 µtm. POC quot of ech strin s function of within the Azores (), Bergen (b) nd Cnry Islnds (c) popultions. PIC quot of ech strin s function of within the Azores (d), Bergen (e) nd Cnry Islnds (f) popultions. For more detil informtion, see Figure 3.
growth rte (d 1 ) growth rte (d 1 ) 1. 1.3 1.2 1.1 1. () y =.25 * x +.1 R 2 =.5.9 1. 1.3 1.2 1.1 1. 6 1 1 1 2 POC production rte (pg C cell 1 d 1 ) (b) y =.13 * x +.722 R 2 =.72.9 6 1 1 1 2 POC quot (pg C cell 1 ) Figure S5. Incresed growth rtes of 17 E. huxleyi strins with incresing POC production rtes () or POC quots (b).
PIC : POC rtio 2. Azores Bergen Cnry Islnds 1.6.. ( tm) Figure S6. Responses of PIC : POC rtio of the Azores (squre), Bergen (circulr) nd Cnry Islnds (dimond) popultions to CO 2 rnge from µtm to 263 µtm.
PIC : POC rtio PIC : POC rtio PIC : POC rtio 2. 1.6 A23 A22 A21 A19 A13 A1 ().. 1.2 (b).9.6 B95 B63 B62 B51 B1 B17.3 3 2 (c) C9 C91 C9 C1 C35 1 ( tm) Figure S7. Responses of PIC : POC rtio of individul E. huxleyi strins in the Azores (), Bergen (b) nd Cnry Islnds (c) popultions to CO 2 rnge from 115 µtm to 37 µtm.
References Locrnini, R. A., Mishonov, A. V., Antonov, J. I., Boyer, T. P., nd Grci, H. E.: World ocen tls 25, V. 1: Temperture, in: NOAA Atls NESDIS 61, edited by Levitus, S., U.S. Government Printing Office, 3 13, 26. Smuelsen, T. J.: The biology of six species of Anomur (Crustce, Decpod) from Runefjorden, western Norwy, Srsi, 5, 25 52, 197. Sntn-Csino, J. M., González-Dávil, M., Rued, M., Llinás, O., nd González-Dávil, E.: The internnul vribility of ocenic CO 2 prmeters in the northest Atlntic subtropicl gyre t the ESTOC site, Glob. Biogeochem. Cycles, 21, GB115, doi: 1.129/26GB27, 27. Wisshk, M., Form, A., Jkobsen, J., nd Freiwld, A.: Temperte crbonte cycling nd wter mss properties from intertidl to bthyl depths (Azores), Biogeosciences, 7, 2379 2396, doi: 1.519/bg-7-2379-21, 21.