CONTACT DETAILS AND ORDER INFORMATION

SWEDISH EXCELLENCE IN NANOPORUS SILICA SVEA (U)HPLC s CONTACT DETAILS AND ORDER INFORMATION Nanologica AB Forskargatan 0G SE-5 8 SÖDERTÄLJE Sweden + 6 8 0 79 9 info@nanologica.com www.nanologica.com

ABOUT SVEA COLUMNS Nanologica offers best-in-class (U)HPLC analytical columns that provide excellent chromatographic performance with sharp peak shapes and robust performance under extreme ph conditions (< and > 0 respectively). The unique surface chemistry and controlled particle properties of Nanologica's proprietary silica result in low back pressures and high plate numbers. With an exceptionally strong silica backbone SVEA columns offer long life cycles. SVEA columns gives excellent selectivity across a wide range of chemistry needs. We take pride in the quality, design and performance of our products. They embody our core value: Swedish Excellence in Nanoporous Silica.

Nanologica offers best-in-class (U)HPLC with a broad portfolio of bonded phases. PHASES C8 Gold C8 Opal C8 C Phenyl Hexyl PFP Cyano PARTICLE SIZES.6 µm.5 µm 5 µm COLUMN IDs. mm.0 mm.6 mm 0 mm COLUMN LENGTHS 50 mm 50 mm 00 mm 50 mm

Content From Silica to s Silica Production and Functionalisation 5 6 Reproducibility Durability 7 8 Bed Stability and Life Cycle 9 Selection Guide SVEA C8 Gold 0 SVEA C8 Opal SVEA C8 SVEA C 6 SVEA Phenyl Hexyl 7 SVEA PFP 8 SVEA Cyano 9 SVEA Core 0 SVEA Core C8 SVEA Core Phenyl Hexyl Article Numbers 5

FROM SILICA TO COLUMNS Nanologica has been producing, modifying and coating silica for several years. Modern technology and demanding quality control is deployed at each step of the manufacturing process to ensure highest possible product performance. The extensive experience and knowledge in silica chemistry, along with internal control of the entire value chain, guarantees exceptional quality and excellent batch to batch reproducibility. Excellent chromatographic performance Bonded phases using proprietary methods Proprietary manufacturing of high purity silica Controlled particle size and particle size distribution Nanologica 5

SILICA PRODUCTION AND FUNCTIONALISATION Nanologica manufactures spherical porous silica particles with controlled pore size, particle size, and particle size distribution resulting in excellent chromatographic properties. The Scanning Electron Microscope (SEM) image below shows perfect spherical shapes and narrow particle size distribution with no fines or crushed particles. The magnified image shows perfectly smooth silica surfaces with no irregularities. Nanologica offers a range of phases with different and complementary chromatographic properties. The functionalisation is performed using proprietary production protocols to produce densely functionalised and end-capped silica particles with low residual silanol activity. The coated silica particles exhibit excellent chromatographic performance and outstanding chemical stability. Stationary phase Chemical structure End capped USP code C8 Gold C8 Opal Coreshell C8 Yes L C8 Yes L7 C Yes L6 Phenyl Hexyl Coreshell Phenyl Hexyl Yes L PFP (Pentafluorophenyl) Yes L Cyano Yes L0 6 Nanologica

REPRODUCIBILITY Nanologica's coating shows high batch to batch reproducibility for both retention times and efficiencies. : SVEA C8 Gold 50x.6 mm 5 µm : Acetonitrile/H₂O 70/0% :.0 ml/min : 0 0C : UV 0 nm Analytes:. Uracil. Toluene. Propylbenzene 6 7 5 9 8 Batch 0 5 6 7 8 9 0 Nanologica 7

DURABILITY The SVEA columns show excellent durability in harsh acidic as well as harsh basic conditions. Both efficiencies and retention times remain almost unaffected even after more than 7 000 column volumes, as shown in the stability tests below. ACIDIC CONDITIONS Analyte : SVEA C8 Gold 00x.6 mm 5 µm : A - % TFA in water, ph 0.9 B - % TFA in acetonitrile :.0 ml/min : 600C : Ethylbenzene Gradient cycle : 0-90% B in 5 min 90% B for min 90-0% B in min 0% B for min BASIC CONDITIONS Analyte : SVEA C8 Gold 00x.6 mm 5 µm : A - 0 mm ammonium bicarbonate, ph 9.6 B - Acetonitrile :.0 ml/min : 50C : Progesterone Gradient cycle : 0-90% B in 5 min 90% B for min 90-0% B in min 0% B for min 8 Nanologica

BED STABILITY Bed stability testing shows maintained efficiency and stable back pressure after close to 00 000 column volumes. BED STABILITY Analyte : SVEA C8 Gold 00x.0 mm 5 µm : 0 mm Potassium phosphate buffer at ph.7/meoh 0/60 : 0.5 ml/min : 00C : Amitriptyline Naphthalene COLUMN LIFE CYCLE The long life cycle of the SVEA columns is demonstrated by preserved separation capacity even after 700 column injections when analysing a food sample. LIFE CYCLE Analyte : SVEA C8 Gold 50x.6 mm 5 µm : Methanol: 0mmol/L Ammonium Acetate=0:80 (v:v) :.0 ml/min : 50C : Acesulfame potassium Benzoic acid Sorbic acid Sodium saccharide Separation of acesulfame potassium, benzoic acid, sodium saccharin and sorbic acid. Analytical sample prepared from Ansai honey. Data kindly provided by SinoUnison Technology Co., Ltd., China. mau 50 0nm,nm 5 0 5 0. Acesulfame potassium. Benzoic acid. Sodium saccharin. Sorbic acid 5 0 5 Pump A pressure MPa 90 80 70 60 50 0 5 0 5 0 5 mau 0nm,nm. Acesulfame potassium. Benzoic acid. Sodium saccharin. Sorbic acid Pump A pressure MPa 90 80 70 60 50 0 0 0 0 0 5 0 5 0 0 0-5 0.0.0.0.0.0 5.0 6.0 7.0 8.0 9.0 0.0 min 0 0.0.0.0.0.0 5.0 6.0 7.0 Separation after one injection using a new SVEA C8 Gold column. Separation after 700 injections. Nanologica 9

COLUMN SELECTION GUIDE Different kinds of functionalisation offer different interaction mechanisms between the stationary phase and analytes to fit a wide range of applications. The figure below is a guideline for choosing the right type of bonded phase depending on the interaction between the analyte and the stationary phase. HYDROPHOBICITY LOW HIGH Cyano C PFP Phenyl Hexyl C8 C8 π-π INTERACTIONS WEAK STRONG C8 C8 C Cyano Phenyl Hexyl PFP ELECTROSTATIC/DIPOLE INTERACTIONS WEAK STRONG C8 C8 C Phenyl Hexyl Cyano PFP HYDROGEN BONDING WEAK STRONG C8 C8 C Phenyl Hexyl Cyano PFP 0 Nanologica

SVEA C8 Gold Silica : Type B Silica Particle Size :.5, 5 µm Surface Area : 00 m /g Pore Size : 0 Å Pore Volume : 0.85 ml/g Carbon Load : 9% Ligand Density :.7 µmol/m Bonded Phase : Dimethyloctadecylsilane End-capping : Yes USP Code : L ph Range : -0 General fi rst choice column High hydrophobic retention Wide range of analytes Excellent peak shape for acids and bases SVEA C8 Gold is the first-choice LC column for a wide range of analytes. The high carbon load provides high retention and selectivity for compounds with moderate to high lipophilicity. Thorough end-capping combined with very low acidity and homogenously distributed residual silanol groups result in excellent peak shape and efficiencies with bases as well as acidic compounds. Comparison of peak shapes and retention times of Nortriptyline and Amitriptyline Acetylsalicylic acid and related compounds : SVEA C8 Gold 50x.6 mm 5 µm : 0% 5 mm KH PO ph 7.0 80% methanol : ml/min : 00C : UV 0 nm : SVEA C8 Gold 50x.6 mm 5 µm : 60% 0.% H PO 0% acetonitrile : ml/min : 00C : UV 7 nm. Nortriptyline. Amitriptyline SVEA C8 Gold Competitor A Competitor B. -hydroxy benzoic acid. Acetylsalicylic acid. Salicylic acid 5 6 7 8 9 0 Thorough end-capping and low polarity of the silica surface of SVEA C8 Gold gives significantly better peak shapes of anti-depressants, compared to competitor brands. 0 5 6 High separation efficiency and symmetrical peak shapes. The tailing factor for salicylic acid is 0.96. Order Information SVEA C8 Gold s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm 5 µm.0 A5V A55V A56V A58V.6 A55V A555V A565V A585V.5 µm. AV A5V A6V -.0 AV A5V A6V A8V.6 A5V A55V A65V A85V Nanologica

Water soluble vitamins Clozapine and related impurities Gradient : SVEA C8 Gold 50x.6 mm 5 µm : A 5 mm KH PO ph.6 B acetonitrile : 5-0% B in 8 min :.5 ml/min : 50C. Ascorbic acid. Nicotinic acid. Pyridoxamine. Nicotinamide Gradient : SVEA C8 Gold 00x.6 mm.5 µm : A 0.% TFA in H O B 0.% TFA in acetonitrile : 0-0% B in min; 0-95% B in min; 95% in min.... Impurity C Clozapine Impurity D Impurity A :.5 ml/min 5. Impurity B : 50C : UV 57 nm 5 5. Folic acid 6. Cyanocobalamine 7. Riboflavin 7 5 6 0 5 6 7 8 0 5 6 7 8 9 0 Sharp peaks and selectivities of vitamins. The high efficiency of SVEA C8 Gold.5 µm gives an extraordinary resolution profile over the forced degradation sample of Clozapine. Low ph stability at high temperature Gradient Cycle : SVEA C8 Gold 50x.6 mm 5 µm : A % TFA in H O ph 0.9 B % TFA in acetonitrile : 0-90% B in 5 min; 90% B in min 90-0% B in min; 0% B in min :.0 ml/min : 600C : UV 5 nm % Initial efficiency 0 00 80 60 0 0 0 % Initial efficiency Retention time 0 000 000 000 000 5000 6000 7000 Volumes,0,0 0,0 9,0 8,0 7,0 6,0 5,0 Retention time No change in either efficiency or retention time for ehylbenzene after running gradient cycles at ph 0.9 and 60 C for more than 7000 column volumes. Nanologica

SVEA C8 Opal Silica Particle Size Surface Area Pore Size Pore Volume Carbon Load Ligand Density Bonded Phase End-capping USP Code ph Range Recommended for high ph applications Proprietary coating ensures solely hydrophobic interaction Better peak shape for ionisable compounds Low bleeding of ligands : Type B Silica :.5, 5 µm : 00 m /g : 0 Å : 0.85 ml/g : 9% :.7 µmol/m : Octadecyl silane : Yes : L : - SVEA C8 Opal is coated with a proprietary bonding technology, which provides a fully covered silica surface. The column material is protected against hydrolysis of the ligands at low ph and silica dissolution at high ph. The coating results in only hydrophobic interactions, resulting in excellent peak shape for all types of analytes. The proprietary bonding technology binds the ligands strongly, providing exceptionally low bleeding. Separation of Nortriptyline and Amitriptyline High ph stability at high temperature : SVEA C8 Opal 50x.6 mm 5 µm : 0% 5 mm KH₂PO₄ ph 7.0 80% methanol : ml/min : 00C. Nortriptyline : UV 0 nm. Amitriptyline : SVEA C8 Opal 50x.6 mm 5 µm : A 0 mm NH₄HCO₄ ph 0.5 B acetonitrile Gradient : 0-50% B in 60 min Gradient Cycle : 0-90% B in min; 90% B in 5 min; 90-0% B in min; 0% B in 5 min : ml/min : 600C : UV 0 nm 00 % of initial efficiency 80 60 0 0 Amitriptyline Progesterone 6 8 0 6 8 0 Highly efficient and symmetrical peak shapes for Nortriptyline and Amitriptyline. Tailing factor for Nortriptyline is.08, and for Amitriptyline.0. 0 0 000 000 000 000 5000 6000 7000 Volumes The efficiencies of the neutral (Progesterone) and basic (Amitriptyline) compounds are almost unaffected using gradient cycles after more than 7000 column volumes. Order Information SVEA C8 Opal s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm 5 µm.0 A5V A55V A56V A58V.6 A55V A555V A565V A585V.5 µm. AV A5V A6V -.0 AV A5V A6V A8V.6 A5V A55V A65V A85V Nanologica

SVEA C8 Silica Particle Size Surface Area Pore Size Pore Volume Carbon Load Ligand Density Bonded Phase End-capping USP Code ph Range : Type B Silica :.5, 5 µm : 00 m /g : 0 Å : 0.85 ml/g : % :.7 µmol/m : Dimethyloctylsilane : Yes : L7 : -9 Similar selectivity for lipophilic compounds as C8 Gold Lower retention than C8 Gold Slightly diff erent selectivity for ionized acids and bases compared to SVEA C8 Gold Excellent peak shape for acids and bases An alternative media to SVEA C8 Gold that gives lower retention. Due to the more hydrophilic nature of the bonded phase, ionized acids and especially bases can have better peak shapes and diff erent selectivity compared to SVEA C8 Gold. Recommended for mixture containing moderately polar and very hydrophobic compounds. Comparison of peak shapes and retention times between C8 and C8 Gold for two anti-depressants : SVEA C8 50x.6 mm 5 µm and C8 Gold 5 µm : 0% 5 mm KH₂PO₄ ph 7.0 80% methanol : ml/min : 00C : UV 0 nm. Nortriptyline. Amitriptyline C8 5 µm C8 Gold 5 µm Water soluble organic acids : SVEA C8 50x.6 mm 5 µm : 97% 5 mm KH₂PO₄ ph.5 % methanol : ml/min. Tartaric acid : 50C. Malic acid : UV 0 nm. Lactic acid. Maleic acid 5. Fumaric acid 5 5 6 7 8 9 0 The excellent peak shapes for ionized compounds with SVEA C8 is revealed by analysing anti-depressants. 5 6 7 8 9 Excellent peak shapes and selectivities of water soluble organic acids are obtained at highly hydrophilic elution conditions. 0 Order Information SVEA C8 s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm 5 µm.0 B5V B55V B56V B58V.6 B55V B555V B565V B585V.5 µm. BV B5V B6V -.0 BV B5V B6V B8V.6 B5V B55V B65V B85V Nanologica

Comparison of the elution order of phenols between C8 and C8 Gold bonded silica : SVEA C8 50x.6 mm 5 µm and SVEA C8 Gold 50x.6 mm 5 µm Gradient : A % AcOH in H₂O B % AcOH in methanol : C8: 5% B in 0 min C8 Gold: 60% B in 6 min 5-90% B in 0 min 60-80% B in min : ml/min : 00C : UV 80 nm. Phenol. -Nitrophenol. -Nitrophenol 5. -Chlorophenol 5.,-Dichlorophenol C8 C8 Gold 5 5 7 9 5 7 9 5 7 9 5 7 9 C8 is more hydrophilic than C8 Gold as seen by the reversal of the elution order of -nitrophenol and -chlorophenol. Clozapine system suitability test Atorvastatin (Lipitor) system suitability test : SVEA C8 50x.6 mm 5 µm : 0% 0.8% Et₃N in H₂O 80% methanol : ml/min : 00C : UV 57 nm. Impurity C. Impurity D. Impurity A. Clozapine 5. Unknown : SVEA C8 00x.6 mm.5 µm : 58% 50 mm NH₄OAc ph.6 0% acetonitrile, % THF :. ml/min : 00C : UV nm. Impurity A. Impurity B. Atorvastatin. Impurity C Rs,=6. Rs, =.5 5 5 5 6 7 8 Sharp peaks and resolution. 5 6 7 8 9 0 Atorvastatin and its diastereomeric Impurity B are seperated well using SVEA C8.5 µm. Nanologica 5

SVEA C Silica Particle Size Surface Area Pore Size Pore Volume Carbon Load Ligand Density Bonded Phase End-capping USP Code ph Range : Type B Silica :.5, 5 µm : 00 m /g : 0 Å : 0.85 ml/g : 7% :.7 µmol/m : Dimethyloctylsilane : Yes : L6 : -8 Recommended for separation of large peptides and proteins Very low retention for lipophilic compounds Can also be run in HILIC-mode Recommended for extremely lipophilic compounds to reduce analytical time. Excellent starting point for analysing peptide and protein mixtures. For intermediately polar analytes, such as amino acids, SVEA C can also be run in HILIC-mode. Separation of Nortriptyline and Amitriptyline Peptide mix separation : SVEA C 50x.6 mm 5 µm : 0% 5 mm KH₂PO₄ ph 7.0 80% methanol : ml/min : 00C : UV 0 nm. Nortriptyline. Amitryptiline Gradient : SVEA C 50x.6 mm 5 µm : A 0.% TFA in H₂O B 0.085% TFA in acetonitrile : 0-50% B in 60 min : ml/min : 00C : UV 5 nm. AA, pl.5. 9 AA, pl.7. AA, pl. (AA=# amino acid residues in peptide, pi=isoelectric point) 0 5 Short retention times and symmetrical peaks of the basic anti-depressants obtained on SVEA C. 0 5 0 5 0 5 0 5 0 5 50 55 60 Separation of three diff erent peptides on SVEA C. Order Information SVEA C s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm 5 µm.0 C5V C55V C56V C58V.6 C55V C555V C565V C585V.5 µm. CV C5V C6V -.0 CV C5V C6V C8V.6 C5V C55V C65V C85V 6 Nanologica

SVEA Phenyl Hexyl Silica Particle Size Surface Area Pore Size Pore Volume Carbon Load Ligand Density Bonded Phase End-capping USP Code ph Range : Type B Silica :.5, 5 µm : 00 m /g : 0 Å : 0.85 ml/g : 6% :.8 µmol/m : Dimethylphenylhexylsilane : Yes : L : -8 Orthogonal chemistry for method development Can be used in aquous conditions Recommended for separation of aromatics and/or polar analytes The aromatic ring and the alkyl chain will give a mixed interaction; π-π and hydrophobic interaction, respectively. Good choice as an orthogonal column compared to SVEA C8/C8 in method development, where the traditional alkyl-based stationary phases fail to provide adequate separation. This media can be used in highly aqueous conditions (00 % wettability), especially for very polar compounds. Analyses of various phenols on Phenyl Hexyl and C8 Gold bonded silica : SVEA PhHex 50x.6 mm 5 µm and SVEA C8 Gold 50x.6 mm 5 µm : A % AcOH in H₂O B % AcOH in methanol Gradients : PhHex: 5% B in 0 min C8: 60% B in 6 min 5-90% B in 0 min 60-80% B in min : ml/min : 00C : UV 80 nm 5. Phenol. -Nitrophenol. -Chlorophenol. -Nitrophenol 5.,-Dichlorophenol PhHex C8 Gold 5 0 6 8 0 6 8 0 0 6 8 0 6 8 0 Selectivity diff erence between Phenyl Hexyl and C8 Gold for separation of phenols. Order Information SVEA Phenyl Hexyl s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm 5 µm.0 F5V F55V F56V F58V.6 F55V F555V F565V F585V.5 µm. FV F5V F6V -.0 FV F5V F6V F8V.6 F5V F55V F65V F85V Nanologica 7

SVEA PFP Silica Particle Size Surface Area Pore Size Pore Volume Carbon Load Ligand Density Bonded Phase End-capping USP Code ph Range : Type B Silica :.5, 5 µm : 00 m /g : 0 Å : 0.85 ml/g : % :.9 µmol/m : Dimethylpentafluorophenylpropylsilane : Yes : L : -8 Strong retention of protic compounds and analytes with high dipole moments Strong π-interaction with electron deficient aromatic rings Recommended for very polar compounds Due to the highly electron rich nature of the aromatic rings of SVEA PFP, the stationary phase interacts strongly with analytes containing polar aprotic and electron deficient aromatic moieties. Additionally, the highly electronegative surface of the aromatic ring provides strong hydrogen bonding with analytes with protic moieties, such as hydroxyl groups and carboxylic acids. The delocalized charge over the fluorine-carbon bond will interact with analytes containing dipole moments. The polar nature of SVEA PFP ensures a fully wettable stationary phase, making it suitable for analysing very polar compounds. Comparison between PFP and C8 Gold bonded phases Comparison between PFP and C8 Gold bonded phases : SVEA PFP 50x.6 mm 5 µm and SVEA C8 Gold 5 µm : 70% H₂O 0% methanol : ml/min. Caffeine : 00C. Amitryptyline : UV 0 nm : SVEA PFP 50x.6 mm 5 µm and SVEA C8 Gold 5 µm : 60% H₂O 0% methanol : ml/min : 00C : UV 0 nm,. p-cresol. m-cresol. o-cresol PFP C8 Gold PFP C8 Gold 0 5 6 7 8 9 0 Strong hydrogen bonding of the PFP column: the hydrogen bonding acceptor, caffeine, and the donor, phenol, has much higher retention than for the C8 Gold. 0 6 8 0 6 8 0 The SVEA PFP successfully separates three cresol isomers, compared to C8 Gold. Order Information SVEA PFP s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm 5 µm.0 P5V P55V P56V P58V.6 P55V P555V P565V P585V.5 µm. PV P5V P6V -.0 PV P5V P6V P8V.6 P5V P55V P65V P85V 8 Nanologica

SVEA Cyano Silica : Type B Silica Particle Size :.5, 5 µm Surface Area : 00 m /g Pore Size : 0 Å Pore Volume : 0.85 ml/g Carbon Load : 7% Ligand Density :.7 µmol/m Bonded Phase : -Cyanopropyldimethylsilane End-capping : Yes USP Code : L0 ph Range : -7.5 Very polar stationary phase Strong dipole-dipole interactions Orthogonal phase in RPLC method development Recommended for HILIC and Normal Phase Recommended for analytes having too high retention on an alkyl-based stationary phase, as well as mixtures of very polar and lipophilic analytes. The nitrile group of the stationary phase interacts favourably with analytes containing double and/or triple bonds, making SVEA Cyano suitable for unsaturated compounds. Due to its very polar nature, SVEA Cyano can be used in both HILIC-mode as well as in normal phase chromatography. Please refer to the care and use instructions for additional information. Separation of two isomeric polar organic acids Benzylamine analysed on Cyano bonded silica : SVEA CN 50x.6 mm 5 µm : 97% 0 mm NH₄OAc ph.9 % methanol : ml/min : 00C : UV 0 nm. Maleic acid. Fumaric acid : SVEA CN 50x.6 mm 5 µm : 70% 0 mm KH₂PO₄ ph 7.6 0% methanol : ml/min : 00C : UV 0 nm. Benzylamine. Phenol 0 5 6 Very short analytical time and base line separation between the two watersoluble cis-/trans-isomeric acids. 0 5 6 7 8 9 0 Sharp and symmetrical peaks of basic and highly polar benzylamine. Order Information SVEA Cyano s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm 5 µm.0 Y5V Y55V Y56V Y58V.6 Y55V Y555V Y565V Y585V.5 µm. YV Y5V Y6V -.0 YV Y5V Y6V Y8V.6 Y5V Y55V Y65V Y85V Nanologica 9

SVEA Core SVEA CORE SVEA Core is a core shell product which consists of a solid core particle coated with a layer of porous silica, as illustrated below. A key feature of core shell columns is the much narrower particle size distribution compared to the fully porous materials. This results in less space among particles in the column and an increased efficiency. For example, columns packed with μm core shell particles produce efficiencies approaching those packed with μm fully porous particles, but at significantly lower back pressures. 0.5 µm.6 µm.6 µm Core Porous layer SVEA Core properties: Exceptional peak shape for basic, acidic and chelating compounds High stability at low and high ph (-0) Excellent back pressure profile 0 Nanologica

SVEA Core C8 Silica Particle Size Surface Area Pore Size Pore Volume Carbon Load Ligand Density Bonded Phase End-capping USP Code ph Range Core shell technology provides high separation and low back pressures Recommended for UPLC applications Excellent base stability : Type B Silica :.6 µm : 0-50 m /g : 80-00 Å : 0.5-0. ml/g : 6-8% :.5 µmol/m : Octadecylsilane : Proprietary : L : -0 The superfi cially porous silica layer is bonded with the same proprietary chemistry as SVEA C8 Opal, providing outstanding peak shapes for ionisable compounds and good selectivity for all types of analytes. Core shell technology provides similar efficiencies as sub µm particles while retaining the back pressure of a µm particle, making it possible to run shorter columns on an ordinary HPLC (0-00 mm length). Comparison between SVEA Core C8.6 µm and a competitor core shell C8.6 µm column Comparison between SVEA Core C8.6 µm and a competitor core shell C8.6 µm column Gradient : SVEA Core C8 50x.6 mm : A 0.% Formic acid in H₂O B methanol : 0% B in min; 0-90% B in min; 90% B in min : 0.5 ml/min : Ambient : MS/MS.. Tetracyclin Doxycyclin e Gradient : SVEA Core C8 50x.6 mm : A 0.% Formic acid in H₂O B methanol : 0% B in min; 0-90% B in min; 90% B in min : 0.5 ml/min : Ambient : MS/MS Nalidixic acid SVEA Core C8 Competitor SVEA Core C8 Competitor 0 5 6 7 8 9 The nice peak shapes from the SVEA Core C8 column give better quantifi cations of the compounds of interest. 0 5 6 7 8 9 The SVEA Core C8 column is exhibiting superior chromatographical behaviour compared to the competitor core shell column. 0 Data kindly provided by Animal, Plant and Food Inspection Center APFIC Jiangsu Entry-Exit Inspection and Quarantine Bureau of People s Republic of China Data kindly provided by Animal, Plant and Food Inspection Center APFIC Jiangsu Entry-Exit Inspection and Quarantine Bureau of People s Republic of China Order Information SVEA Core C8 s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm.6 µm. AV6 A5V6 A6V6 -.0 AV6 A5V6 A6V6 -.6 A5V6 A55V6 A65V6 - Nanologica

β-blockers Cis/trans-isomers of 9-octadecenoic acid : SVEA Core C8 00x.6 mm : 50% 5 mm KH₂PO₄ ph 7.0 50% methanol : ml/min : 00C : UV 80 nm. Atenolol. Metoprolol. Timolol. Pronanolol : SVEA Core C8 50x.6 mm : 0% 0.5% Formic acid in H₂O 90% acetonitrile : ml/min : 00C : UV 5 nm. Oleic acid. Elaidic acid 0 5 6 7 8 The complete coating of the SVEA Core C8 column gives excellent peaks of highly basic compounds. 0 5 6 7 Base-line separation between the cis/trans-isomeric acids. Nanologica

SVEA Core Phenyl Hexyl Silica Particle Size Surface Area Pore Size Pore Volume Carbon Load Ligand Density Bonded Phase End-capping USP Code ph Range : Type B Silica :.6 µm : 0-50 m /g : 80-00 Å : 0.5-0. ml/g : -5% :. µmol/m : Phenyl hexyl silane : Proprietary : L :.5-9 Orthogonal chemistry combined with core shell technology Can be used in aquous conditions Recommended for separation of aromatics and/or polar analytes Combining Core shell technology with orthoganal ligand chemistry. The aromatic ring will interact with aromatic and polar compounds, through its π-system. Components of explosives Calcium antagonists : SVEA Core Phenyl Hexyl 00x.6 mm : 60% water 0% acetonitrile : ml/min : 00C : UV 50 nm.,-dinitrobenzene.,,5-trinitrobenzene.,-dinitrotoluene.,,6-trinitrotoluene : SVEA Core Phenyl Hexyl 00x.6 mm : 0% 0 mm KH₂PO₄ ph 6.8 70% methanol : ml/min : 00C : UV 0 nm. Nifedipine. Nitrinedipene. Diltiazem. Verapamil 5. Nicardipine 5 0 5 6 7 8 Separation of structurally similar aromatic compounds using SVEA Core Phenyl Hexyl column. 0 6 8 0 Excellent chromatografi cal performance is exhibited by the SVEA Core Phenyl Hexyl column. Order Information SVEA Core Phenyl Hexyl s Length Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm.6 µm. FV6 F5V6 F6V6 -.0 FV6 F5V6 F6V6 -.6 F5V6 F55V6 F65V6 - Nanologica

Nanologica

Article Numbers SVEA s Length Bonded Phase C 8 Gold C 8 Opal Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm 5 µm.0 A5V A55V A56V A58V.6 A55V A555V A565V A585V.5 µm. AV A5V A6V -.0 AV A5V A6V A8V.6 A5V A55V A65V A85V 5 µm.0 A5V A55V A56V A58V.6 A55V A555V A565V A585V.5 µm. AV A5V A6V -.0 AV A5V A6V A8V.6 A5V A55V A65V A85V C 8 C 5 µm.5 µm 5 µm.5 µm.0 B5V B55V B56V B58V.6 B55V B555V B565V B585V. BV B5V B6V -.0 BV B5V B6V B8V.6 B5V B55V B65V B85V.0 C5V C55V C56V C58V.6 C55V C555V C565V C585V. CV C5V C6V -.0 CV C5V C6V C8V.6 C5V C55V C65V C85V Ph Hex PFP Cyano 5 µm.0 F5V F55V F56V F58V.6 F55V F555V F565V F585V.5 µm. FV F5V F6V -.0 FV F5V F6V F8V.6 F5V F55V F65V F85V 5 µm.0 P5V P55V P56V P58V.6 P55V P555V P565V P585V.5 µm. PV P5V P6V -.0 PV P5V P6V P8V.6 P5V P55V P65V P85V 5 µm.0 Y5V Y55V Y56V Y58V.6 Y55V Y555V Y565V Y585V.5 µm. YV Y5V Y6V -.0 YV Y5V Y6V Y8V.6 Y5V Y55V Y65V Y85V SVEA Core s Length Bonded Phase C 8 Ph Hex Particle Size ID (mm) 50 mm 00 mm 50 mm 50 mm.6 µm. AV6 A5V6 A6V6 -.0 AV6 A5V6 A6V6 -.6 A5V6 A55V6 A65V6 -.6 µm. FV6 F5V6 F6V6 -.0 FV6 F5V6 F6V6 -.6 F5V6 F55V6 F65V6 - Nanologica 5

Guard s - units/pack Bonded Phase C 8 C 8 C Ph Hex PFP Cyano Particle Size ID (mm) Article number 5 µm.0 A50V.0 A50V.5 µm.0 A0V.0 A0V 5 µm.0 B50V.0 B50V.5 µm.0 B0V.0 B0V 5 µm.0 C50V.0 C50V.5 µm.0 C0V.0 C0V 5 µm.0 F50V.0 F50V.5 µm.0 F0V.0 F0V 5 µm.0 P50V.0 P50V.5 µm.0 P0V.0 P0V 5 µm.0 Y50V.0 Y50V.5 µm.0 Y0V.0 Y0V Accessories Product Guard Holder Core Guard Holder Core Guard Filter Demo Kit Content Core Guard Holder 5 units/pack Guard Holder unit/pack Guard Holder unit/pack Demo Kit units/pack Article number V000V V000V6 X000V6 D000V Photo credits: Dr. Jan Blid and Kunal Mukhopadhyay; Nanologica Copyright Nanologica 6 Nanologica