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Rolling Provincial Review: Implementation 2001-2003 Province 229

Columbia Basin Fish & Wildlife Authority Province FY 2001-2003 Spending Summaries NPCC Recommendations and BPA Spending by Project Category, FY01-03 Habitat $7,171,442 $19,735,751 Monitoring $2,637,595 $2,508,874 Production $7,503,336 $6,208,883 Research & Evaluation $820,608 $572,047 NPCC Recommended BPA Spent $0 $5,000,000 $10,000,000 $15,000,000 $20,000,000 $25,000,000 NPCC Recommendations and BPA Spending by Project Type, FY01-03 Anadromous $199,973 $134,816 Resident $21,040,047 $16,326,430 Wildlife $0 $9,457,270 NPCC Recommended BPA Spent $0 $5,000,000 $10,000,000 $15,000,000 $20,000,000 $25,000,000 230

Rolling Provincial Review: Implementation 2001-2003 Bitterroot Subbasin 231

Columbia Basin Fish & Wildlife Authority Blackfoot Subbasin 232

Rolling Provincial Review: Implementation 2001-2003 Projects in the Blackfoot Subbasin Project ID Project Title Review Cycle BiOp? 200200700 Restoring Bull Trout Habitat in The Blackfoot River's North Fork Rec 00-03 $ 0 $330,000 $10,000 Type Category Accuracy Spent 01-03 -- $ 0 $ 0 $ 0 Resident Habitat area no 233

Columbia Basin Fish & Wildlife Authority Clark Fork Subbasin 234

Rolling Provincial Review: Implementation 2001-2003 Flathead Subbasin 235

Columbia Basin Fish & Wildlife Authority Projects in the Flathead Subbasin Project ID Project Title Review Cycle BiOp? 199101901 Research, Monitor, and Restore Native Species no Rec 00-03 $166,048 $131,400 $144,500 Type Category Accuracy Spent 01-03 -- $231,379 $109,910 $196,592 Resident Habitat subbasin 199101903 Hungry Horse Mitigation yes Rec 00-03 $781,432 $982,850 $990,000 Type Category Accuracy Spent 01-03 -- $910,298 $821,492 $1,149,401 Resident Habitat area 199101904 Stocking of offsite waters for Hungry Horse Mitigation - Creston National Fish Hatchery Rec 00-03 $160,000 $106,672 $109,872 Type Category Accuracy Spent 01-03 -- $232,082 $268,024 $128,067 Resident Production area 199502500 Flathead River Instream Flow FY 2000 no Rec 00-03 $ 0 $ 0 $ 0 Type Category Accuracy Spent 01-03 -- $58,843 $17,013 $138,037 Resident Habitat area 199608701 Focus Watershed Coordination-Flathead River Watershed FY 2001 no Rec 00-03 $65,303 $ 0 $ 0 Type Category Accuracy Spent 01-03 -- $59,987 $32,261 $102,398 Resident Habitat stream 200200300 Secure and Restore Critical Fish and Wildlife Habitats no Rec 00-03 $ 0 $4,918,444 $4,538,826 Type Category Accuracy Spent 01-03 -- $ 0 $ 0 $ 0 Wildlife Habitat area 200204200 Riparian Habitat Preservation - Weaver Slough and McWinegar Slough Rec 00-03 $ 0 $1,002,000 $ 0 Type Category Accuracy Spent 01-03 -- $ 0 $ 0 $ 0 Resident Habitat area no no Projects in bold have preliminary results data included in this report. 236

Rolling Provincial Review: Implementation 2001-2003 199101904 Stocking of Offsite Waters for Hungry Horse Mitigation 2002 Project Objectives Rear up to 100,000 westslope cutthroat trout annually for offsite mitigation stocking Rear up to 100,000 rainbow trout annually for offsite mitigation in closed-basin waters Fish Stockings - Preliminary Results Westslope Cutthroat Trout CSKT Managed Waters - Upper Jocko Reservoir 3,000 - Lower Jocko Reservoir 7,000 - Swartz Lake 5,000 - Turtle Lake 3,000 - Lower Twin Lake 2,000 - Upper Twin Lake 2,000 MFWP Managed Waters - Bailey Lake 2,000 - Dollar Lake 1,000 - Hidden Lakes 2,500 - Whitefish Lake 25,000 - Upper Whitefish Lake 10,000 - Lion Lake 3,000 - Bootjack Lake 1,000 - Myron Lake 750 Photo: Courtesy U. S. Fish and Wildlife Service U.S. Fish and Wildlife Service employee culturing westslope cutthroat trout and rainbow trout at the Creston National Fish Hatchery. Rainbow Trout CSKT Managed Waters - Pablo Reservoir 25,000 - McDonald Ponds 5,000 MFWP Managed Waters -McGregor Lake 75,000 Photo: Courtesy U. S. Fish and Wildlife Service Release of hatchery-reared rainbow trout and westslope cutthroat trout in closed-basin lakes in the Flathead Subbasin. 237

Columbia Basin Fish & Wildlife Authority 199101903 Hungry Horse Mitigation 2002 Project Objectives Assess distribution and movements of juvenile and adult bull trout, westslope cutthroat trout, rainbow trout, and westslope cutthroat x rainbow trout hybrids in the mainstem, North, and Middle forks of the Flathead River Eliminate or suppress hybridized or non-native populations to reduce negative species interactions with native westslope cutthroat trout and bull trout Improve fish habitat and passage problems in streams throughout the Flathead Basin Develop habitat suitability curves for bull trout and westslope cutthroat trout required by the Instream Flow Incremental Methodology on the Flathead River Distribution of Westslope Cutthroat Trout and Hybrids - Preliminary Results Hybridization between westslope cutthroat trout and rainbow trout was confirmed in 26 of 47 sample locations Surveys illustrated occurrence of hybridization between westslope cutthroat trout and rainbow trout in tributaries of the North Fork Flathead River previously void of such events Status of hybridization between rainbow trout and westslope cutthroat trout in the North Fork Flathead River. Locations in the Flathead River basin where hybridization occurs between westslope cutthroat trout and rainbow trout.. Example of a westslope cutthroat trout x rainbow trout hybrid. 238

Rolling Provincial Review: Implementation 2001-2003 Activities to Suppress Non-native Fishes - Preliminary Results Lower Basin Tributaries Barriers Fish and redd removal Upper Basin Lakes/Tributaries Chemical eradication Barriers Methods used to control non-native fishes in the Fathead Subbasin include redd removal and barriers. Eradication/control efforts initiated in 1999 Control efforts have resulted in a reduction of rainbow trout and rainbow trout x westslope cutthroat trout hybrids from an average of 88 fish/km of stream (1997-2000) to 28 fish/km (2002-2003) in the Flathead River Number of fish/km 300 250 200 150 100 50 0 <200 mm 200-400 mm >400 mm 244 166 145 121 87 96 97 62 53 42 28 27 12 18 10 3.8 2.5 4.7 1997 1998 2000 2001 2002 2003 Reductions in the numbers of rainbow trout and westslope cutthroat trout x rainbow hybrids per km of stream as a result of control/ eradication efforts implemented in 1999. 239

Columbia Basin Fish & Wildlife Authority Fish Passage Improvements and Population Responses - Preliminary Results Fish Passage Seven fish passage enhancement projects completed from 1994-1997 Redds now identified above all previous barriers Projects resulted in the availability of 16% more habitat # of WCT/150 m 200 Culvert Replaced 150 100 50 0 1992 1994 1996 1998 2000 Westslope cutthroat trout population estimates for Murray Creek following the replacement of faulty culverts. Prior to replacement (upper photo), the Felix Creek culvert prevented fish from moving upstream. Fish Habitat Improvements and Population Responses - Preliminary Results July 2000 Restored floodplain Stabalized channel Installed 50 habitat unit structures Juvenile westslope cutthroat trout density doubled Number of WCT/150 m 160 140 120 100 80 60 40 20 Treatment 0 July 1998 1999 2000 2001 2002 Year Emery Creek before (upper) and after flood restoration.. 240 Juvenile westslope cutthroat trout densities before and after restoration of Emery Creek.

Rolling Provincial Review: Implementation 2001-2003 Habitat Availability Related to Discharge - Preliminary Results Useable habitat area for subadult bull trout in the Flathead River below Hungry Horse Reservoir is significantly reduced as discharge (cfs) occurs A significant loss of habitat area occurs with a discharge of only 5000 cfs Photo: Courtesy Montana Fish, Wildlife, Parks Montana Fish, Wildlife, and Parks studies have shown that water releases from Hungry Horse Reservoir limit available habitat for subadult bull trout. Useable Habitat Area for Flathead River Subadult Bull Trout Under Different Discharges 241

Columbia Basin Fish & Wildlife Authority Kootenai Subbasin 242

Rolling Provincial Review: Implementation 2001-2003 Projects in the Kootenai Subbasin Project ID Project Title Review Cycle BiOp? 198806400 Kootenai River White Sturgeon Studies and Conservation Aquaculture Rec 00-03 $1,128,568 $1,160,000 $2,999,000 Type Category Accuracy Spent 01-03 -- $1,247,674 $1,651,384 $1,228,444 Resident Production stream 198806500 Kootenai River Fisheries Recovery Investigations yes Rec 00-03 $570,000 $825,391 $1,057,804 Type Category Accuracy Spent 01-03 -- $701,039 $717,181 $863,592 Resident Monitoring area 199404900 Improving the Kootenai River Ecosystem yes Rec 00-03 $273,333 $710,891 $855,000 Type Category Accuracy Spent 01-03 -- $271,655 $471,285 $710,268 Resident Production subbasin 199500400 Mitigation for the Construction and Operation of Libby Dam Rec 00-03 $795,000 $805,000 $830,000 Type Category Accuracy Spent 01-03 -- $611,681 $928,768 $966,672 Resident Habitat area 199608702 Focus Watershed Coordination in the Kootenai River Watershed no Rec 00-03 $100,000 $101,500 $101,750 Type Category Accuracy Spent 01-03 -- $14,453 $97,952 $104,486 Resident Habitat area 200000400 Monitor and Protect Bull Trout for Koocanusa Reservoir yes Rec 00-03 $60,400 $62,000 $62,000 Type Category Accuracy Spent 01-03 -- $47,382 $56,178 $123,502 Resident Monitoring area 200006800 Impact of Flow Regulation on Riparian Cottonwood Ecosystems FY 2000 no Rec 00-03 $199,973 $ 0 $ 0 $ 0 Type Category Accuracy Spent 01-03 -- $134,816 $ 0 $ 0 Anadromous 200101300 Evaluate the Effects of Nutrient Supplementation on Benthic Periphyton, Macroinvertebrates, and Juvenile Sturgeon in the Kootenai River Research & Evaluation FY01 Innovative yes yes stream Rec 00-03 $170,635 $ 0 $ 0 Type Category Accuracy Spent 01-03 -- $ 0 $69,491 $95,812 Resident 200200200 Assess Feasibility of Enhancing White Sturgeon Spawning Substrate Habitat, Kootenai R., Idaho Research & Evaluation no stream Rec 00-03 $ 0 $350,000 $100,000 Type Category Accuracy Spent 01-03 -- $ 0 $ 0 $271,928 Resident Projects in bold have preliminary results data included in this report. Research & Evaluation yes stream 243

Columbia Basin Fish & Wildlife Authority Projects in the Kootenai Subbasin, continued... Project ID Project Title Review Cycle BiOp? 200200800 Reconnection of Floodplain Slough Habitat to the Kootenai River Rec 00-03 $ 0 $139,974 $540,000 Type Category Accuracy Spent 01-03 -- $ 0 $ 0 $98,956 Resident Habitat area no 200201100 Implement Floodplain Operational Loss Assessment, Protection, Mitigation and Rehabilitation on the Lower Kootenai River Watershed Ecosystem no Rec 00-03 $ 0 $293,864 $612,500 Type Category Accuracy Spent 01-03 -- $ 0 $75,914 $444,949 Resident Habitat area 200203900 Smith Creek Restoration no Rec 00-03 $ 0 $52,680 $302,680 Type Category Accuracy Spent 01-03 -- $ 0 $ 0 $ 0 Resident Habitat stream 200204400 Purchase Conservation Easement from Plum Creek Timber Company (PCT) along Fisher River Rec 00-03 $ 0 $500,000 $500,000 Type Category Accuracy Spent 01-03 -- $ 0 $ 0 $ 0 Resident Habitat area no 244

Rolling Provincial Review: Implementation 2001-2003 199500400 Mitigation for the Construction and Operation of Libby Dam Libby Mitigation 2002 Project Objectives Complete habitat restoration projects in Grave, Libby, Upper Libby creeks to enhance native species in the Kootenai Subbasin Develop a genetic conservation reserve of native interior redband trout to be used as the initial source of eggs for reintroduction following eradication efforts in local lakes Habitat Restoration Projects Preliminary Results Graves Creek Irrigation Diversion Prior to improvements, the Graves Creek diversion which was created in 1923, produced 2,000 cubic yards of sediment annually. 245

Columbia Basin Fish & Wildlife Authority Habitat Restoration Projects Preliminary Results Continued Rock Weirs Fish Screens Headgate and Trash Rack To improve fish habitat, and associated populations, weirs, screens and headgates were installed in Graves Creek. Physical Monitoring - Graves Creek Parameter Pre-Project Post- Project Bankfull Width (ft) 45-240 50-54 Width/Depth Ratio 93.5 10 Sinuosity 1.15 1.4 Bankfull Depth (ft) 1.2 2.3 Pool Spacing (ft) 670 430 Channel Morphology Changes Increases Maximum and mean depth Reductions Cross section area, bankful width, and width/depth ratio Fisheries Habitat Improvements Pool length 17% increase Mean pool depth 37% increase Maximum pool depth 54% increase Number Fish. per 1000 Feet. 35 30 25 20 15 10 5 0 2000-2001 2002 Years Cutthroat Trout Rainbow Trout Brook Trout 246

Rolling Provincial Review: Implementation 2001-2003 Habitat Restoration Projects Preliminary Results Continued Libby Creek (River Mile 12) Results Restoration Efforts The following structures were installed to assist in moving the creek away from the sloughing bank: Rock J-hook vanes (N = 7) Log/rootwad complexes ( N = 7) More than 3,000 trees Prior to restoration, 5,900 yards of sediment was released annually from the incised bank (right side of photo). Corrective measures have moved the creek and streambank incision has been eliminated. Aerial view shows the creek no longer flows directly into the bank (area circled). Physical Monitoring - Graves Creek Parameter Pre-Project Post-Project Bankfull Width (ft) 45-240 50-54 Width/Depth Ratio 93.5 10 Sinuosity 1.15 1.4 Bankfull Depth (ft) 1.2 2.3 Pool Spacing (ft) 670 430 247

Columbia Basin Fish & Wildlife Authority Habitat Restoration Projects Preliminary Results Continued Libby Creek (River Mile 22) Results Restoration Efforts Restoration efforts included the use of the following structures and vegetation: Upper Libby Creek in 2002 prior to restoration efforts. Cobble-grade control structures (N = 11) Rootwad/logjam complexes (N = 19) Shrub transplants (N = 500) Willow plantings ( N = 2,000) Cottonwood plantings (N = 75) Containerized native shrubs ( N = 1,600) Parameter Upper Libby Creek during restoration. Pre-Project PostProject Total Length 2,700 ft. Braided 3,200 ft. Bankfull Width 27-63 ft. 28-35 ft. Mean Depth 1.2 ft. 2.1 ft. Max. Depth 2.2 ft. 3.0 ft. Width/Depth ratio Sinuosity 15-35 20-23 1.1 1.6 Pool Spacing 247 ft. 80 ft. Upper Libby Creek following restoration efforts 248

Rolling Provincial Review: Implementation 2001-2003 Lake Rehabilitation Projects and Genetic Conservation Suppression efforts directed towards nonnative fishes were completed at the following closed-basin lakes in the Flathead River subbasin: Bootjack Lake Cibid Lake Topless Lake Carpenter Lake Banana Lake Spring Creek Banana Lake receiving chemical treatments to suppress non-native fish species. In an attempt to preserve the unique genetic stocks of redband trout that exist in the lakes of the Flathead subbasin, specimens were collected from the lakes prior to rehabilitation. The use of artificial production and the rearing of offspring in natural conditions are techniques that are being used to enable biologists to restock the treated lakes with an ample number of fish that are fit for their new environments, an approach that will maximize survival following stocking. 249

Columbia Basin Fish & Wildlife Authority 198806400 Kootenai River White Sturgeon Studies and Conservation 2002 Project Objectives Propagate and rear up to 12 families of white sturgeon per year from wild Kootenai River broodfish Monitor and evaluate genetic variability and diversity of wild white sturgeon broodstock and their hatchery- produced progeny Monitor and evaluate survival, growth, and condition of hatchery-reared juvenile white sturgeon Artificial Production of Kootenai River White Sturgeon - Preliminary Results Spawned 112 Kootenai River white sturgeon (36 females and 76 males) from 1990-2002 76 families produced from 1990-2002 Released over 20,000 juvenile white sturgeon from 1992-2002 Annual number of families ranged from 1 11 from 1990 2002 Egg to larval survival of 1.8 86% from 1990-2002 Percent egg to larval survival. 100 90 80 70 60 50 40 30 20 10 0 Percent Survival No. Families 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 2002 12 10 8 6 4 2 0 number of families. Year Percent egg to larval survival for Kootenai River white sturgeon reared at the Kootenai Hatchery 8000 7000. Numnbers released 6000 5000 4000 3000 2000 1000 0 1990 1991 1992 1993 1994 1995 1996 1997 1998 1999 2000 2001 Year Class Annual releases of Kootenai River white sturgeon reared at the Kootenai Hatchery 250

Rolling Provincial Review: Implementation 2001-2003 Genetic Variability and Diversity of Broodstock and Progeny - Preliminary Results Comparison of haplotype frequencies between the wild population and the subset of Kootenai Hatchery broodstock, (1997 through 2000). Haplotype frequencies have remained relatively constant Percent 60 50 40 30 20 48.15 47.32 25.92 31.25 Wild fish (n=112) Hatchery broodstock (n=54) The breeding program has been successful in maintaining, in the hatchery broodstock, the variability and diversity found in wild Kootenai River white sturgeon Diversity has remained constant with no variants lost 10 11.11 9.82 9.26 5.56 5.36 6.25 0 LV01 LV02 LV03 LV04 LV05 Haplotype (Length variant) Frequency Survival, Growth, Condition, and Habitat Use - Preliminary Results 60% (+ 10%) survival of released fish after one year Photo: Courtesy Kootenai Tribe of Idaho 90% (+ 10%) survival from year two and later Average growth 2.5 in/year Following adaptation to the wild, hatchery reared white sturgeon exhibit excellent survival, significant improvements in growth and improved condition factors Juvenile Kootenai River white sturgeon reared at the Kootenai Hatchery. 251

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