Safety data sheet according to 1907/2006/EC, Article 31 Print date: 01.08.2013 Updated: 01.08.2013 1. Identification of the substance/mixture and of the company/undertaking Product identifier Trade name: TRANSCRIPTME RNA KIT Article number: RT31-020, RT31-100, RT31-S Relevant identified uses of the substance or mixture and uses advised against: no data available Application of the substance / the preparation: Pharmaceutical analysis, Biochemistry Components: TRANSCRIPTME Enzyme Mix, 2x RT Master Mix, RNase H (E. coli), Nuclease-free water Details of the supplier of the safety data sheet Manufacturer/supplier: BLIRT S.A. Trzy Lipy 3/1.38 80-172, Gdansk info@dnagdansk.com Emergency telephone number: 112 1
Safety data sheet according to 1907/2006/EC, Article 31 Print date: 01.08.2013 Updated: 01.08.2013 1. Identification of the substance/mixture and of the company/undertaking Product identifier Trade name: 2x RT Master Mix Relevant identified uses of the substance or mixture and uses advised against: no data available Application of the substance / the preparation: Laboratory chemicals Details of the supplier of the safety data sheet Manufacturer/supplier: BLIRT S.A. Trzy Lipy 3/1.38 80-172, Gdansk info@dnagdansk.com Emergency telephone number: 112 2. Hazards identification Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Classification according to Directive 67/548/EEC or Directive 1999/45/EC The preparation is classified as dangerous according to Directive 1999/45/EC and its amendments Label elements Labelling according to Regulation (EC) No 1272/2008: none Hazard pictograms: none Signal word: none Hazard statements: none Precautionary statement(s): none Other hazards Not applicable. 2
3. Composition/information on ingredients Chemical characterization: Mixture Ingredient name CAS number EC number Classification Tris-HCl 1185-53-1 214-684-5 Xi; R36/38 Dithiothreitol 3483-12-3 222-468-7 Xn; Xi; R22; R36/38 4. First aid measures Description of first aid measures General information: No special measures required. After inhalation: Follow standard care for a respiratory emergency as necessary. Remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen. Get medical attention immediately. After skin contact: Immediately wash with water and soap and rinse thoroughly. After eye contact: Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor. After swallowing: If symptoms persist consult doctor. Information for doctor Most important symptoms and effects, both acute and delayed: No further relevant information available. Indication of any immediate medical attention and special treatment needed: No further relevant information available. 5. Firefighting measures Extinguishing media Suitable extinguishing agents: water spray, CO 2, foam, powder. Special hazards arising from the substance or mixture Special hazard arising from the mixture: none known Advise for firefighters Protective equipment: Wear self-contained respiratory protective device. Additional information Dispose of fire debris and contaminated fire fighting water in accordance with official regulations. 6. Accidental release measures 3
Personal precautions, protective equipment and emergency procedures: Put on appropriate personal protective equipment. Environmental precautions: Avoid dispersal of split material and runoff and contact with solis, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution. Methods and material for containment and cleaning up: Pick up mechanically. 7. Handling and storage Handling Precautions for safe handing: The substances should be handled with care and under precautions to avoid inhalation. Information about fire and explosion protection: The product is not flammable. Conditions for safe storage, including any incompatibilities Requirements to be met by storerooms and receptacles: No special requirements. Information about storage in one common storage facility: Not required. Further information about storage conditions: none Recommended storage temperature: -20 o C Storage class: 10-13 Specific end use(s): No further relevant information available. 8. Exposure controls/personal protection Control parameters Ingredients with limit values that require monitoring at the workplace: Not required. Additional information: The lists valid during the making were used as basis. Exposure controls Personal protective equipment: General protective and hygienic measures: The usual precautionary measures are to be adhered to when handling chemicals. Respiratory protection: Not required. Protection of hands: Protective gloves the glove material has to be impermeable and resistant to the product/the substance/the preparation. Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation: Material of gloves: the selection of suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer. Penetration time of glove material: The exact break through time has to be observed. 4
For the permanent contact gloves made of the following materials are suitable: Nitrile rubber, NBR. Recommended thickness of the material 0,11 mm. Value for the permeation: Level 480 min. Eye protection: Safety glasses. Body protection: Protective work clothing. Protective clothing should be selected specifically for the working place, depending on concentration and quantity of the hazardous substances handled. 9. Physical and chemical properties Information on basic physical and chemical properties Appearance: Form: Liquid (noncombustible/flameproof) Other information: No further relevant information available 10.Stability and reactivity Reactivity: no data available Chemical stability: the product is stable at -20 0 C for three years Thermal decomposition/conditions to be avoided: No decomposition if used according to specifications. Possibility of hazardous reaction: no dangerous reactions known Conditions to avoid: no further relevant information available Incompatible materials: no further relevant information available Hazardous decomposition product: See chapter 5 11.Toxicological information Information on toxicological effects Primary irritant effect: Inhalation: Irritating effect On the skin: May be harmful by skin absorption. May cause skin irritation On the eye: Irritating effect Sensitization: no sensitizing effects known 12.Ecological information Toxicity: no data available Persistence and degradability: no further relevant information available Bioaccumulative potential: no further relevant information available Mobility in soil: no further relevant information available Results of PBT and vpvb: not applicable Other adverse effects: no further relevant information available 5
General notes: Water hazard class 1: slightly hazardous for water. Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system. 13. Disposal considerations Waste treatment methods Recommendation: Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additional handling information and protection of employees. Uncleaned packaging (Recommendation): Disposal must be made according to official regulations. Recommended cleansing agents: Water, if necessary together with cleansing agents. 14. Transport information UN-Number: ADR, ADN, IMDG, IATA: not dangerous goods/not applicable UN proper shipping name: ADR, ADN, IMDG, IATA: not dangerous goods/not applicable Transport hazard class(es): ADR, ADN, IMDG, IATA: not dangerous goods/not applicable Clasa: not dangerous goods/not applicable Packing group: ADR, IMDG, IATA: not dangerous goods/not applicable Environmental hazards: Marine pollutant: No Special precautions for users: not applicable Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code: not applicable Transport/additional information: not dangerous according to the above specifications. UN Model Regulation : not applicable 15.Regulatory information Chemical safety assessment: A Chemical Safety Assessment has not been carried out. 16. Other information 6
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship. Safety data sheet according to 1907/2006/EC, Article 31 Print date: 01.08.2013 Updated: 01.08.2013 1. Identification of the substance/mixture and of the company/undertaking Product identifier Trade name: TRANSCRIPTME Enzyme Mix Relevant identified uses of the substance or mixture and uses advised against: no data available Application of the substance / the preparation: Laboratory chemicals Details of the supplier of the safety data sheet Manufacturer/supplier: BLIRT S.A. Trzy Lipy 3/1.38 80-172, Gdansk info@dnagdansk.com Emergency telephone number: 112 2. Hazards identification Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Classification according to Directive 67/548/EEC or Directive 1999/45/EC The preparation is classified as dangerous according to Directive 1999/45/EC and its amendments Label elements Labelling according to Regulation (EC) No 1272/2008: none Hazard pictograms: none Signal word: none Hazard statements: none Precautionary statement(s): none Other hazards Not applicable. 7
3. Composition/information on ingredients Chemical characterization: Mixture Ingredient name CAS number EC number Classification Glycerol 56-81-5 200-289-5 Xi; R36/37/38 4. First aid measures Description of first aid measures General information: No special measures required. After inhalation: Follow standard care for a respiratory emergency as necessary. Remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen. Get medical attention immediately. After skin contact: Immediately wash with water and soap and rinse thoroughly. After eye contact: Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor. After swallowing: If symptoms persist consult doctor. Information for doctor Most important symptoms and effects, both acute and delayed: No further relevant information available. Indication of any immediate medical attention and special treatment needed: No further relevant information available. 5. Firefighting measures Extinguishing media Suitable extinguishing agents: water spray, CO 2, foam, powder. Special hazards arising from the substance or mixture Special hazard arising from the mixture: none known Advise for firefighters Protective equipment: Wear self-contained respiratory protective device. Additional information Dispose of fire debris and contaminated fire fighting water in accordance with official regulations. 6. Accidental release measures Personal precautions, protective equipment and emergency procedures: Put on appropriate personal protective equipment. 8
Environmental precautions: Avoid dispersal of split material and runoff and contact with solis, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution. Methods and material for containment and cleaning up: Pick up mechanically. 7. Handling and storage Handling Precautions for safe handing: The substances should be handled with care and under precautions to avoid inhalation. Information about fire and explosion protection: The product is not flammable. Conditions for safe storage, including any incompatibilities Requirements to be met by storerooms and receptacles: No special requirements. Information about storage in one common storage facility: Not required. Further information about storage conditions: none Recommended storage temperature: -20 o C Storage class: 10-13 Specific end use(s): No further relevant information available. 8. Exposure controls/personal protection Control parameters Ingredients with limit values that require monitoring at the workplace: Not required. Additional information: The lists valid during the making were used as basis. Exposure controls Personal protective equipment: General protective and hygienic measures: The usual precautionary measures are to be adhered to when handling chemicals. Respiratory protection: Not required. Protection of hands: Protective gloves the glove material has to be impermeable and resistant to the product/the substance/the preparation. Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation: Material of gloves: the selection of suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer. Penetration time of glove material: The exact break through time has to be observed. 9
For the permanent contact gloves made of the following materials are suitable: Nitrile rubber, NBR. Recommended thickness of the material 0,11 mm. Value for the permeation: Level 480 min. Eye protection: Safety glasses. Body protection: Protective work clothing. Protective clothing should be selected specifically for the working place, depending on concentration and quantity of the hazardous substances handled. 9. Physical and chemical properties Information on basic physical and chemical properties Appearance: Form: Liquid (noncombustible/flameproof) Other information: No further relevant information available 10.Stability and reactivity Reactivity: no data available Chemical stability: the product is stable at -20 0 C for three years Thermal decomposition/conditions to be avoided: No decomposition if used according to specifications. Possibility of hazardous reaction: no dangerous reactions known Conditions to avoid: no further relevant information available Incompatible materials: no further relevant information available Hazardous decomposition product: See chapter 5 11.Toxicological information Information on toxicological effects Primary irritant effect: Inhalation: Irritating effect On the skin: Irritating effect On the eye: Irritating effect Sensitization: no sensitizing effects known 12.Ecological information Toxicity: no data available Persistence and degradability: no further relevant information available Bioaccumulative potential: no further relevant information available Mobility in soil: no further relevant information available Results of PBT and vpvb: not applicable Other adverse effects: no further relevant information available 10
General notes: Water hazard class 1: slightly hazardous for water. Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system. 13.Disposal considerations Waste treatment methods Recommendation: Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additional handling information and protection of employees. Uncleaned packaging (Recommendation): Disposal must be made according to official regulations. Recommended cleansing agents: Water, if necessary together with cleansing agents. 14.Transport information UN-Number: ADR, ADN, IMDG, IATA: not dangerous goods/not applicable UN proper shipping name: ADR, ADN, IMDG, IATA: not dangerous goods/not applicable Transport hazard class(es): ADR, ADN, IMDG, IATA: not dangerous goods/not applicable Clasa: not dangerous goods/not applicable Packing group: ADR, IMDG, IATA: not dangerous goods/not applicable Environmental hazards: Marine pollutant: No Special precautions for users: not applicable Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code: not applicable Transport/additional information: not dangerous according to the above specifications. UN Model Regulation : not dangerous goods/not applicable 15.Regulatory information Chemical safety assessment: A Chemical Safety Assessment has not been carried out. 16.Other information 11
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship. Safety data sheet according to 1907/2006/EC, Article 31 Print date: 01.08.2013 Updated: 01.08.2013 1. Identification of the substance/mixture and of the company/undertaking Product identifier Trade name: RNase H (E. coli) Relevant identified uses of the substance or mixture and uses advised against: no data available Application of the substance / the preparation: Laboratory chemicals Details of the supplier of the safety data sheet Manufacturer/supplier: BLIRT S.A. Trzy Lipy 3/1.38 80-172, Gdansk info@dnagdansk.com Emergency telephone number: 112 2. Hazards identification Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 Not a hazardous substance or mixture according to Regulation (EC) No. 1272/2008. Classification according to Directive 67/548/EEC or Directive 1999/45/EC The preparation is classified as dangerous according to Directive 1999/45/EC and its amendments Label elements Labelling according to Regulation (EC) No 1272/2008: none Hazard pictograms: none Signal word: none Hazard statements: none Precautionary statement(s): none 12
Other hazards Not applicable. 3. Composition/information on ingredients Chemical characterization: Mixture Ingredient name CAS number EC number Classification Glycerol 56-81-5 200-289-5 Xi; R36/37/38 4. First aid measures Description of first aid measures General information: No special measures required. After inhalation: Follow standard care for a respiratory emergency as necessary. Remove to fresh air. If not breathing give artificial respiration. If breathing is difficult, give oxygen. Get medical attention immediately. After skin contact: Immediately wash with water and soap and rinse thoroughly. After eye contact: Rinse opened eye for several minutes under running water. If symptoms persist, consult a doctor. After swallowing: If symptoms persist consult doctor. Information for doctor Most important symptoms and effects, both acute and delayed: No further relevant information available. Indication of any immediate medical attention and special treatment needed: No further relevant information available. 5. Firefighting measures Extinguishing media Suitable extinguishing agents: water spray, CO 2, foam, powder. Special hazards arising from the substance or mixture Special hazard arising from the mixture: none known Advise for firefighters Protective equipment: Wear self-contained respiratory protective device. Additional information Dispose of fire debris and contaminated fire fighting water in accordance with official regulations. 6. Accidental release measures 13
Personal precautions, protective equipment and emergency procedures: Put on appropriate personal protective equipment. Environmental precautions: Avoid dispersal of split material and runoff and contact with solis, waterways, drains and sewers. Inform the relevant authorities if the product has caused environmental pollution. Methods and material for containment and cleaning up: Pick up mechanically. 7. Handling and storage Handling Precautions for safe handing: The substances should be handled with care and under precautions to avoid inhalation. Information about fire and explosion protection: The product is not flammable. Conditions for safe storage, including any incompatibilities Requirements to be met by storerooms and receptacles: No special requirements. Information about storage in one common storage facility: Not required. Further information about storage conditions: none Recommended storage temperature: -20 o C Storage class: 10-13 Specific end use(s): No further relevant information available. 8. Exposure controls/personal protection Control parameters Ingredients with limit values that require monitoring at the workplace: Not required. Additional information: The lists valid during the making were used as basis. Exposure controls Personal protective equipment: General protective and hygienic measures: The usual precautionary measures are to be adhered to when handling chemicals. Respiratory protection: Not required. Protection of hands: Protective gloves the glove material has to be impermeable and resistant to the product/the substance/the preparation. Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation: Material of gloves: the selection of suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer. 14
Penetration time of glove material: The exact break through time has to be observed. For the permanent contact gloves made of the following materials are suitable: Nitrile rubber, NBR. Recommended thickness of the material 0,11 mm. Value for the permeation: Level 480 min. Eye protection: Safety glasses. Body protection: Protective work clothing. Protective clothing should be selected specifically for the working place, depending on concentration and quantity of the hazardous substances handled. 9. Physical and chemical properties Information on basic physical and chemical properties Appearance: Form: Liquid (noncombustible/flameproof) Other information: No further relevant information available 10.Stability and reactivity Reactivity: no data available Chemical stability: the product is stable at -20 0 C for three years Thermal decomposition/conditions to be avoided: No decomposition if used according to specifications. Possibility of hazardous reaction: no dangerous reactions known Conditions to avoid: no further relevant information available Incompatible materials: no further relevant information available Hazardous decomposition product: See chapter 5 11.Toxicological information Information on toxicological effects Primary irritant effect: Inhalation: Irritating effect On the skin: Irritating effect On the eye: Irritating effect Sensitization: no sensitizing effects known 12.Ecological information Toxicity: no data available Persistence and degradability: no further relevant information available Bioaccumulative potential: no further relevant information available Mobility in soil: no further relevant information available 15
Results of PBT and vpvb: not applicable Other adverse effects: no further relevant information available General notes: Water hazard class 1: slightly hazardous for water. Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system. 13.Disposal considerations Waste treatment methods Recommendation: Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additional handling information and protection of employees. Uncleaned packaging (Recommendation): Disposal must be made according to official regulations. Recommended cleansing agents: Water, if necessary together with cleansing agents. 14.Transport information UN-Number: ADR, ADN, IMDG, IATA: not dangerous goods/not applicable UN proper shipping name: ADR, ADN, IMDG, IATA: not dangerous goods/not applicable Transport hazard class(es): ADR, ADN, IMDG, IATA: not dangerous goods/not applicable Clasa: not dangerous goods/not applicable Packing group: ADR, IMDG, IATA: not dangerous goods/not applicable Environmental hazards: Marine pollutant: No Special precautions for users: not applicable Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code: not applicable Transport/additional information: not dangerous according to the above specifications. UN Model Regulation : void 15.Regulatory information Chemical safety assessment: A Chemical Safety Assessment has not been carried out. 16.Other information 16
This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship. Safety data sheet according to 1907/2006/EC, Article 31 Print date: 01.08.2013 Updated: 01.08.2013 1. Identification of the substance/mixture and of the company/undertaking Product identifier Trade name: Nuclease-free water Relevant identified uses of the substance or mixture and uses advised against: no data available Application of the substance / the preparation: Laboratory chemicals Details of the supplier of the safety data sheet Manufacturer/supplier: BLIRT S.A. Trzy Lipy 3/1.38 80-172, Gdansk info@dnagdansk.com Emergency telephone number: 112 2. Hazards identification Classification of the substance or mixture Classification according to Regulation (EC) No 1272/2008 Not applicable. Classification according to Directive 67/548/EEC or Directive 1999/45/EC Not applicable Label elements Labelling according to Regulation (EC) No 1272/2008: none Hazard pictograms: none Signal word: none Hazard statements: none Precautionary statement(s): none 17
Other hazards Not applicable. 3. Composition/information on ingredients Chemical characterization: Water 4. First aid measures Description of first aid measures General information: No special measures required. After inhalation: Not applicable. After skin contact: Not applicable After eye contact: Not applicable. After swallowing: Not applicable. Information for doctor Most important symptoms and effects, both acute and delayed: No further relevant information available. Indication of any immediate medical attention and special treatment needed: No further relevant information available. 5. Firefighting measures Extinguishing media Suitable extinguishing agents: Not applicable. Special hazards arising from the substance or mixture Special hazard arising from the mixture: Not applicable Advise for firefighters Protective equipment: Not applicable. Additional information Not applicable 6. Accidental release measures Personal precautions, protective equipment and emergency procedures: Put on appropriate personal protective equipment. Environmental precautions: Not applicable. Methods and material for containment and cleaning up: Pick up mechanically. 7. Handling and storage Handling Precautions for safe handing: Not applicable. Information about fire and explosion protection: The product is not flammable. Conditions for safe storage, including any incompatibilities 18
Requirements to be met by storerooms and receptacles: No special requirements. Information about storage in one common storage facility: Not required. Further information about storage conditions: none Recommended storage temperature: -20 o C Storage class: 10-13 Specific end use(s): No further relevant information available. 8. Exposure controls/personal protection Control parameters Ingredients with limit values that require monitoring at the workplace: Not required. Additional information: Not applicable. Exposure controls Personal protective equipment: General protective and hygienic measures: The usual precautionary measures are to be adhered to when handling chemicals. Respiratory protection: Not required. Protection of hands: Protective gloves the glove material has to be impermeable and resistant to the product/the substance/the preparation. Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation: Material of gloves: the selection of suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer. Penetration time of glove material: The exact break through time has to be observed. For the permanent contact gloves made of the following materials are suitable: Nitrile rubber, NBR. Recommended thickness of the material 0,11 mm. Value for the permeation: Level 480 min. Eye protection: Safety glasses. Body protection: Protective work clothing. Protective clothing should be selected specifically for the working place, depending on concentration and quantity of the hazardous substances handled. 9. Physical and chemical properties Information on basic physical and chemical properties Appearance: Form: Liquid (noncombustible/flameproof) 19
Other information: No further relevant information available 10.Stability and reactivity Reactivity: no data available Chemical stability: Not applicable Thermal decomposition/conditions to be avoided: No decomposition if used according to specifications. Possibility of hazardous reaction: no dangerous reactions known Conditions to avoid: no further relevant information available Incompatible materials: no further relevant information available Hazardous decomposition product: See chapter 5 11.Toxicological information Information on toxicological effects Primary irritant effect: Inhalation: Not applicable On the skin: Not applicable On the eye: Not applicable Sensitization: no sensitizing effects known 12.Ecological information Toxicity: no data available Persistence and degradability: no further relevant information available Bioaccumulative potential: no further relevant information available Mobility in soil: no further relevant information available Results of PBT and vpvb: not applicable Other adverse effects: no further relevant information available General notes: Not applicable. 13.Disposal considerations Waste treatment methods Recommendation: Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additional handling information and protection of employees. Uncleaned packaging (Recommendation): Disposal must be made according to official regulations. Recommended cleansing agents: Water, if necessary together with cleansing agents. 20
14.Transport information UN-Number: ADR, ADN, IMDG, IATA: not dangerous goods/not applicable UN proper shipping name: ADR, ADN, IMDG, IATA: not dangerous goods/not applicable Transport hazard class(es): ADR, ADN, IMDG, IATA: not dangerous goods/not applicable Clasa: not dangerous goods/not applicable Packing group: ADR, IMDG, IATA: not dangerous goods/not applicable Environmental hazards: Marine pollutant: No Special precautions for users: not applicable Transport in bulk according to Annex II of MARPOL 73/78 and the IBC Code: not applicable Transport/additional information: not dangerous according to the above specifications. UN Model Regulation : not dangerous goods/not applicable 15.Regulatory information Chemical safety assessment: A Chemical Safety Assessment has not been carried out. 16.Other information This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship. 21