I. Project Title: Karluk Sockeye Smolt Alaska Sustainable Salmon Fund Statement of Work II. Project Number: PCSRF Objective: RM&E III. Principal Investigator Matt Foster, Finfish Research Biologist ADF&G, Commercial Fisheries Division 211 Mission Road Kodiak, AK 99615 Phone: (907) 486-1857 Email: matt.foster@alaska.gov Co-Investigator Tina Fairbanks, Remote Operations Manager Kodiak Regional Aquaculture Association (KRAA) 104 Center Ave., Suite #200 Kodiak, AK 99615 Phone: (907) 486-6555 Email: pch.davis@gci.net IV. Project Period: 5/1/11 11/30/13 V. Project Description 1. Synopsis Karluk Lake is located on the southwest side of Kodiak Island and supports the largest sockeye salmon run in the Kodiak Management Area. Sockeye salmon smolt studies have been conducted sporadically on Karluk Lake since 1961. This project reinstitutes a multi-year Karluk Lake sockeye salmon smolt program to estimate the size and condition of outmigrating smolt; in addition, individual smolt will be collected on an annual basis for isotope analysis. This information will help develop forecasting models for Karluk Lake sockeye salmon to ensure the sustainability of this important subsistence resource. 2. Introduction Previous smolt projects were conducted on Karluk Lake in the 1960s, in the early 1980s, in 1991, and in 1992. From 1999 to 2003, a smolt project was funded by the Kodiak Regional Aquaculture Association (KRAA) and implemented by ADF&G. The smolt project was continued from 2004 to 2006 as part of a larger project funded by the Gulf of Alaska Ecosystem Monitoring (GEM) program. Funding from the GEM project was acquired to initiate a collaborative study with the University of Alaska to study the role of marine derived nutrients in the Karluk watershed, and both typical smolt age and size sampling was conducted in addition to stable isotope analysis. The last officially funded Karluk sockeye salmon smolt project was in 2006; Karluk Sockeye Smolt Page 1 of 9 1/30/2013
this was at a time when the average size of outmigrating sockeye was the smallest in the dataset stretching back to 1961. The Karluk sockeye smolt size was not only much smaller than normal in 2006, but the majority of the fish were age-3. The resultant ocean-age-2 sockeye salmon returning in 2008 marked the beginning of the reduced adult runs to Karluk Lake. With a growing concern about the diminished run strength of Karluk sockeye salmon, the Kodiak National Wildlife Refuge (KNWR) hosted an interagency forum in December 2009 focusing on the problems at Karluk Lake. One outcome of the forum was a discussion between ADF&G and KRAA about reinitiating sockeye salmon smolt sampling at Karluk Lake to help understand the link between low adult returns and smolt size and body condition. In 2010, ADF&G, KRAA, and Koniag Inc. pooled resources and conducted sampling of outmigrating sockeye salmon smolt to successfully estimate size and condition factors at the outlet of Karluk Lake. This project will collect smolt age and size information late May to mid-june at the outlet of Karluk Lake in 2011-2013; in addition, individuals will be collected to expand the time series of stable isotope analyses that began in 1999. 3. Location Latitude: 57.44093 N Longitude: 154.10942 W AKSSF Objective Westward: 2A-4 VI. Objectives 1. Project Objectives Objective 1: Estimate the average weight, length, and condition factor by age and week of outmigrating sockeye salmon at the Karluk Lake outlet annually (late May to mid June) in 2011-2013 Objective 2: Determine the stable isotopic composition (δ 15 N and δ 13 C) of a subsample of juvenile sockeye salmon corresponding to the sampling in Objective 1 Objective 3: Develop and refine the forecasting model predicting outmigration total return from sockeye salmon smolt average size, incorporating multiple brood years, early- and late-run escapement estimates, and winter environmental conditions (precipitation and temperature) 2. Methods The following tasks will be performed for Objective 1 from 2011 to 2013: Task 1: Install and operate fyke nets to capture emigrating sockeye smolt, as follows: Locate an unframed, winged fyke net with detachable cod end approximately 125 meters downstream from the lake outlet on the east bank of the river Install an alternative rigid-frame fyke net on the west bank of the river opposite and downstream of the main fyke net (this net will be fished twice a week (in tandem with the main fyke net) to identify possible biases in samples collected from the main site) Task 2: Enumerate catch by species, as follows: Karluk Sockeye Smolt Page 2 of 9 1/30/2013
Fish the fyke net nightly from 1900 hours until 0500 hours or until a total of 200 sockeye smolt are captured, at which the point the cod end of the fyke net will be detached Transfer the contents of the bucket to the holding box; in the process identify and count the entire catch by species using a small dip net Identify juvenile salmonid species using external characteristics Task 3: Conduct weekly sampling of 280 sockeye salmon smolt for age (scale samples), weight, and length and conduct smolt sampling for a period of three weeks, as follows: Sample 40 sockeye salmon smolt per day, five days per week, for age, weight, and length from the main fyke net Sample 40 sockeye salmon smolt twice a week from the west bank alternative site Task 4: Estimate average weight, length, and condition factor of outmigrating Karluk sockeye salmon smolt by week, as follows: Develop a sample size such that the estimated mean weight of the major age class per strata will be within 5% of the mean length, 2% of the true value with 95% confidence. Calculate condition factor of each individual sockeye salmon smolt Compare samples collected at the alternative fyke net location with samples collected at the main fyke net location The following tasks will be performed on an annual basis for Objective 2 from 2011 to 2013: Task 1: Collect whole fish samples during the three strata of the season and conduct stable isotope analysis, as follows: To continue the annual time series of stable isotope data collected originally as part of the GEM project in 1999, collect smolt samples on a randomly selected subset of samples from the 2-3 main age classes each year during the smolt out-migration The following tasks will be performed over the entirety of the project for Objective 3: Task 1: Develop and refine the forecasting model predicting outmigration total return from sockeye salmon smolt average size by incorporating multiple brood year earlyand late-run escapement estimates and winter environmental conditions Task 2: Develop a generalized Ricker model where the combined brood year escapement for outmigrating age-2 and age-3 sockeye fits the total outmigration year returns; additional terms included in this generalized Ricker model include the following: average size of age-2 outmigrants average size of age-3 outmigrants November-February precipitation by age-2, and age-3 in-gravel brood years Karluk Sockeye Smolt Page 3 of 9 1/30/2013
November-February air temperature by age-2, and age-3 in-gravel brood years Prior to commencement of project activity, PI will obtain ADF&G Fish Collection, Fish Resource, Fish Transport, and/or other required permits, as appropriate. VII. Benefits The age, size, and condition of outmigrating sockeye smolt are the paramount measure of health, and ultimately marine survival, for smolt to adult salmon. The primary benefit of this project is to assist in the conservation of the Karluk Lake sockeye salmon subsistence resource by collecting data on these essential measures of productivity. This project will also develop forecasting models to more accurately predict future salmon returns and assist in escapement goal development. More precise forecasting methods will enhance fishery management and decrease the probability of scenarios that have historically resulted in over-escapement that has been detrimental to the run and resulted in a large foregone harvest. VIII. Products, Milestones, and Timelines May 22-23, 2011: Install fyke nets and water level indicator at the Karluk Lake outlet May 24 - June 15, 2011: Collect 1 st season (3 strata) of smolt data at the Karluk Lake outlet July 2011: Begin development of generalized Ricker model October 1, 2011 - January 30, 2012: Analyze data and write annual report May 22-23, 2012: Install fyke nets and water level indicator at the Karluk Lake outlet May 24 - June 15, 2012: Collect 2nd season (3 strata) of smolt at the Karluk Lake outlet October 1, 2012 - January 30, 2013: Analyze data and write annual report May 22-23, 2013: Install fyke nets and water level indicator at the Karluk Lake outlet May 24 - June 15, 2013: Collect 3 rd season (3 strata) of smolt data at the Karluk Lake outlet October 1, 2013 January 30, 2014*: Analyze data and write annual report August 2013: Finalize development of generalized Ricker model March 30, 2014*: Provide final report to AKSSF *Costs incurred for activities occurring outside the project period are funded separately. PI will submit PCSRF performance metrics, semiannual, and project completion reports according to the AKSSF schedule, as well as copies of any other report/product/ deliverable produced with this funding. All invoices will be submitted for payment within 30 days of the project end date in accordance with the current AKSSF invoicing P&P. Invoices will be sent to: Alaska Department of Fish and Game Alaska Sustainable Salmon Fund Box 115526 Juneau, AK 99811-5526 Karluk Sockeye Smolt Page 4 of 9 1/30/2013
Any report or product distributed as a result of this funding will include the following language: This [report/video/website] was prepared by [recipient/author name] under award #NA10NMF4380428 from the National Oceanic and Atmospheric Administration, U.S. Department of Commerce, administered by the Alaska Department of Fish and Game. The statements, findings, conclusions, and recommendations are those of the author(s) and do not necessarily reflect the views of the National Oceanic and Atmospheric Administration, the U.S. Department of Commerce, or the Alaska Department of Fish and Game. Any displays produced (e.g., signs, interpretive displays, posters) must include logos from both the National Oceanic and Atmospheric Administration and the Alaska Department of Fish and Game (please contact AKSSF staff for graphics). IX. Partners Koniag Incorporated, the majority owner of land surrounding the Karluk watershed, is in full support of this project. In 2010, Koniag Inc. donated use of its land and cabin for the KRAA and ADF&G collaborative pilot project to sample sockeye salmon smolt at the Karluk Lake outlet and will donate the land and cabin again for this project. X. Project Budget Summary FY11 FY12 FY13 FY14 Total 100 Personnel $5,980 $6,125 $6,300 $0 $18,405 300 Contractual $14,250 $15,750 $15,750 $1,500 $47,250 400 Supplies $2,000 $2,000 $2,000 $0 $6,000 Subtotal $22,230 $23,875 $24,050 $1,500 $71,655 600 Indirect $2,412 $2,643 $2,667 $210 $7,932 Total $24,642 $26,518 $26,717 $1,710 $79,587 This project funds the following entities: ADF&G: $64,587 KRAA: $15,000 ADF&G Budget ADF&G FY11 FY12 FY13 FY14 Total 100 Personnel $5,980 $6,125 $6,300 $0 $18,405 300 Contractual $9,250 $10,750 $10,750 $1,500 $32,250 400 Supplies $2,000 $2,000 $2,000 $0 $6,000 Subtotal $17,230 $18,875 $19,050 $1,500 $56,655 600 Indirect @ 14% $2,412 $2,643 $2,667 $210 $7,932 Total $19,642 $21,518 $21,717 $1,710 $64,587 Karluk Sockeye Smolt Page 5 of 9 1/30/2013
ADF&G Budget Narrative: Line 100: Personnel (Salaries calculated with the State salary calculator.) Michelle Moore, Fishery Biologist, is the on-site and off-site project crew leader processing data and estimating ages of smolt: FY11: 1 month @ $5,980/month = $5,980 FY12: 1 month @ $6,125/month = $6,125 FY13: 1 month @ $6,300/month = $6,300 Line 300: Contractual FY11 Seahawk Air Beaver floatplane charter: 2 flights @ $1,600/flight = $3,200 Cabin and land rental from Karluk Wilderness Adventures: $4,250 Island Air charters for principal investigator and project leader: 2 flights @ $900/flight = $1,800 FY12 Seahawk Air Beaver floatplane charter: 2 flights @ $1,600/flight = $3,200 Stable isotope analysis by Idaho State University: $1,500 Cabin and land rental from Karluk Wilderness Adventures: $4,250 Island Air charters for principal investigator and project leader: 2 flights @ $900/flight = $1,800 FY13 Seahawk Air Beaver floatplane charter: 2 flights @ $1,600/flight = $3,200 Stable isotope analysis by Idaho State University: $1,500 Cabin and land rental from Karluk Wilderness Adventures: $4,250 Island Air charters for principal investigator and project leader: 2 flights @ $900/flight = $1,800 FY14 Stable isotope analysis by Idaho State University: $1,500 Total FY11 Contractual: $9,250 Total FY12 Contractual: $10,750 Total FY13 Contractual: $10,750 Total FY14 Contractual: $1,500 Line 400: Supplies FY11 Food for the field crew, project leader, and biologist: 25 days @ $60/day = $1,500 Scientific supplies (e.g. scale slides, Ziplocs, MS-222, sharpies, aerators, dissecting implements, batteries): $340 Unleaded gasoline for pressure washer and generator: 15 gallons @ $4/gallon = $60 Karluk Sockeye Smolt Page 6 of 9 1/30/2013
#1 Diesel fuel for cabin stove: 20 gallons @ $5/gallon = $100 FY12 Food for the field crew, project leader, and biologist: 25 days @ $60/day = $1,500 Scientific supplies (e.g. scale slides, Ziplocs, MS-222, sharpies, aerators, dissecting implements, batteries): $340 Unleaded gasoline for pressure washer and generator: 15 gallons @ $4/gallon = $60 #1 Diesel fuel for cabin stove: 20 gallons @ $5/gallon = $100 FY13 Food for the field crew, project leader, and biologist: 25 days @ $60/day = $1,500 Scientific supplies (e.g. scale slides, Ziplocs, MS-222, sharpies, aerators, dissecting implements, batteries): $340 Unleaded gasoline for pressure washer and generator: 15 gallons @ $4/gallon = $60 #1 Diesel fuel for cabin stove: 20 gallons @ $5/gallon = $100 Total FY11 Supplies: $2,000 Total FY12 Supplies: $2,000 Total FY13 Supplies: $2,000 Line 600: Indirect ADF&G s federally negotiated indirect rate for FY11 is 15.44%; however, indirect will be recovered at a reduced rate of 14% for this project. KRAA Budget KRAA FY11 FY12 FY13 FY14 Total 100 Personnel $0 $0 $0 $0 $0 300 Contractual $5,000 $5,000 $5,000 $0 $15,000 400 Supplies $0 $0 $0 $0 $0 Total $5,000 $5,000 $5,000 $0 $15,000 Line 100: Personnel Two KRAA interns will staff project: FY11: 4 weeks @ $625/week x 2 people = $5,000 FY12: 4 weeks @ $625/week x 2 people = $5,000 FY13: 4 weeks @ $625/week x 2 people = $5,000 Budget adjustments between line items are allowed for amounts equal to or less than 10% of the total award without prior AKSSF approval; any amount above 10% requires prior AKSSF approval. All costs in lines 100-500 must represent actual cash expenditures. Karluk Sockeye Smolt Page 7 of 9 1/30/2013
All invoices shall be adequately documented with both documentation of expense and proof of payment for both direct and match expenses. Examples of expense documentation include copies of invoices, pay stubs, and receipts. Examples of proof of payment documentation include copies of checks, check numbers, and credit card receipts. Match documentation for expenses equal to or greater than 35% of the reimbursable expenses must be submitted with each invoice. All invoices will be submitted for payment within 30 days of the project end date in accordance with the current AKSSF invoicing P&P. Invoices submitted after that deadline will not be reimbursed. Expenses incurred after the project end date will not be reimbursed. Entities are required to provide current federally negotiated indirect cost rate agreements (NICRAs) throughout the life of the project. Indirect costs will not be reimbursed if NICRAs have expired or if documentation (i.e., the federally approved rate letter) is not provided. Complete AKSSF indirect requirements are available at http://www.adfg.alaska.gov/index.cfm?adfg=akssf.policies. XI. Match Budget Match Summary FY11 FY12 FY13 FY14 Total 100 Personnel $7,528 $7,704 $7,939 $12,599 $35,770 300 Contractual $0 $0 $0 $0 $0 400 Supplies $0 $0 $0 $0 $0 Subtotal $7,528 $7,704 $7,939 $12,599 $35,770 600 Indirect $0 $0 $0 $0 $0 Total $7,528 $7,704 $7,939 $12,599 $35,770* *Match budget shows more match than is required for this project. Total required match: (35%): $27,856 Match may be met by any combination of eligible contributions described below. ADF&G Match Budget ADF&G FY11 FY12 FY13 FY14 Total 100 Personnel $4,053 $4,125 $4,256 $8,812 $21,246 300 Contractual $0 $0 $0 $0 $0 400 Supplies $0 $0 $0 $0 $0 Total Direct Costs $4,053 $4,125 $4,256 $8,812 $21,246 Karluk Sockeye Smolt Page 8 of 9 1/30/2013
ADF&G Match Budget Narrative: Line 100: Personnel (Salaries calculated with the State salary calculator.) Matt Foster will serve as the principal investigator: FY11:.5 months @ $8,106/month = $4,053 FY12:.5 months @ $8,250/month = $4,125 FY13:.5 months @ $8,512/month = $4,256 FY14: 1 month @ $8,812/month = $8,812 KRAA Match Budget KRAA FY11 FY12 FY13 FY14 Total 100 Personnel $3,475 $3,579 $3,683 $3,787 $14,524 300 Contractual $0 $0 $0 $0 $0 400 Supplies $0 $0 $0 $0 $0 Total Direct Costs $3,475 $3,579 $3,683 $3,787 $14,524 KRAA Match Budget Narrative: Line 100: Personnel Tina Fairbanks will serve as the co-investigator: FY11:.5 months @ $6,950/month = $3,475 FY12:.5 months @ $7,158/month = $3,579 FY13:.5 months @ $7,366/month = $3,683 FY14:.5 months @ $7,574/month = $3,787 Karluk Sockeye Smolt Page 9 of 9 1/30/2013