Charles T. Golob, William J. Johnston, and Karine Paré Dept. Crop and Soil Sciences Washington State University Jan. 3, 2008

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Georgia-Pacific s Nitamin Nfusion (25-0-0) Steady-Delivery Nitrogen Soluble Fertilizer Compared to Simplot s Best Polyon (43-0-0) Controlled-Release Fertilizer on a Fairway Cut Kentucky Bluegrass Turf Charles T. Golob, William J. Johnston, and Karine Paré Dept. Crop and Soil Sciences Washington State University Jan. 3, 2008 A field study was conducted at the Washington State University Turfgrass and Agronomy Research Center (TARC) during the summer of 2007 to evaluate the effects of Nitamin Nfusion (25-0-0) on Kentucky bluegrass (cv. Famous ) mowed at fairway height (0.5 ). A randomized-complete block design was used with four replications and individual treatment plots were 6 x 10. Nitamin Nfusion treatments were applied 1 Jun 07 with a bicycle-wheeled CO 2 pressurized (40 psi) sprayer. Nitamin Nfusion alone treatment was diluted 1:1 with water and applied at 64 gal/a using 8006 flat fan spray nozzles at a rate of 2 lbs N/1000 ft 2 and the 4 lbs N/1000 ft 2 Nitamin Nfusion treatment was sprayed 2 times. The Nitamin Nfusion + Urea (46-0-0) treatment was blended so that 50% nitrogen came from each source and applied at 46 gal/a using 11004 flat fan spray nozzles at a rate of 2 lbs N/1000 ft 2 and the 4 lbs N/1000 ft 2 Nitamin Nfusion + Urea treatment was sprayed 2 times. Polyon treatment was applied at the same time using a SS-2 drop spreader. Each week up to 13 weeks after treatment (31 Aug 07) chlorophyll index readings were taken with a Field Scout CM1000 chlorophyll meter (Spectrum Technologies, Inc.). All treatments were mowed 2 times per week at 0.5 with a Jacobsen Greens King IV triplex mower. Immediately prior to the second mowing each week, a 1.75 x 8 swath was mowed at 0.5 with a Jacobsen Greens King IV triplex reel mower to collect clippings. Clippings were dried in a forced air drier at 45 o C for 72 hours then weighed. Visual color and turfgrass quality were rated on a scale of 1-9, with 9 equal to dark green or excellent quality, respectively. Chlorophyll index readings show that the Nitamin Nfusion + Urea 2 lbs N/M had higher index levels of chlorophyll compared to Nitamin Nfusion alone at 2 lbs N/M through 14 Jul 07 (6 WAT) [Table 1 and Figure 1]. This would suggest that the addition of Urea (quick-release) was indeed being taken up by the plant immediately after application. However, at the 4 lbs N/M rate both Nitamin Nfusion alone or with Urea had similar chlorophyll index levels throughout the study. These treatments compared to Polyon at 4 lbs N/M had a higher level of N release from 3 to 6 WAT based on chlorophyll and clipping dry weight (Table 2 and Figure 2). Nitamin Nfusion at 2 lbs N/M, Polyon at the 2 lbs N and 4 lbs N/M did not show an increase in chlorophyll until 5 WAT and did not show a large spike in chlorophyll throughout the study. At 7 WAT, both Polyon treatments had a higher

chlorophyll index than either corresponding Nitamin Nfusion treatment for the remainder of the study, which suggests that N from Polyon is being released at a higher and more prolonged rate than Nitamin Nfusion. As with chlorophyll, there was a corresponding increase of clipping dry weight 3 WAT to 6 WAT with the 4 lbs N/M Nitamin Nfusion alone or with Urea treatments (Table 2 and Figure 2). However, after 8 WAT there was little difference among any of the 4 lbs N/M treatments. This was also true among any of the 2 lbs N/M treatments. Polyon at 4 lbs N/M had significantly less total clippings over the duration of the study than Nitamin Nfusion 4 lbs N/M or Nitamin Nfusion + Urea blend 4 lbs/m (Table 2a). There was no significant difference in total clipping dry weight among any of the 2 lbs N/M treatments. Compared to chlorophyll index (Figure 1), a visual color (Figure 3) or turfgrass quality (Figure 4) spike was not observed, following the 4 lbs N/M Nitamin alone or with Urea treatment. However, an increase in chlorophyll index closely matched an increase in visual color of the 2 lbs N/M Nitamin Nfusion + Urea treatment up to 4 WAT (Figures 1 and 3). In addition, both Polyon treatments matched increases in chlorophyll index throughout the study. By 6 WAT, visual color of the Polyon at 4 lbs N/M treatment was significantly higher compared to the 4 lbs N/M Nitamin Nfusion or the 4 lbs N/M Nitamin Nfusion + Urea treatment (Table 3). The Polyon at 2 lbs/m treatment had significantly higher visual color ratings compared to the 2 lbs N/M rate of Nitamin Nfusion or Nitamin Nfusion + Urea treatments. As explained in the chlorophyll results above, it appears that the N of Polyon is being released at a higher and more prolonged rate than Nitamin Nfusion. Turfgrass quality following either Polyon treatment or Nitamin at 2 lbs N/M did not show an increase until 6 WAT (Figure 4). This response is 3 weeks later than an increase in chlorophyll index and visual color (Figures 1 and 3). Even though color of the turf increased it took another 3 weeks for the turf density to increase resulting in increased turfgrass quality. Turfgrass quality increased immediately after applications of 4 lbs N/M Nitamin Nfusion, 4 lbs N/M Nitamin Nfusion + Urea, or 2 lbs N Nitamin Nfusion + Urea (Figure 4 and Table 4). To summarize, the addition of Urea to the 2 lbs/m rate of the Nitamin Nfusion + Urea treatment had the desired effect as a quick-release of N for plant growth, as seen by increased chlorophyll index and visual color ratings up to 4 WAT, compared to Nitamin Nfusion alone. The controlled-release of N from Polyon was more pronounced than any Nitamin Nfusion treatment. However, it takes up to 4 WAT to see the N release from Polyon. This is also true for Nitamin Nfusion at 2 lbs N/M. Applying Nitamin Nfusion or Nitamin Nfusion + Urea at 4 lbs/m resulted in immediate increases in chlorophyll index, clipping dry weight, visual color, and turfgrass quality. In addition, there was no added benefit of adding Urea at this high N rate, other than it may be less expensive.

Table 1. Effect of different slow-release fertilizers on chlorophyll index of 'Famous' Kentucky bluegrass mowed at fairway height. Chlorophyll index (1-999)* Nitamin Nfusion (25-0-0) 1:1 dilution 2 280.2 307.6 bcd** 323.1 b 345.2 bc 346.5 d 485.2 b 452.5 d 419.9 d 371.1 c 359.5 cd 360.5 cd 339.8 cd 294.3 c 240.8 bc Nitamin Nfusion (25-0-0) 1:1 dilution 4 289.0 347.6 ab 393.5 a 471.8 a 458.4 a 682.0 a 592.7 a 503.4 b 410.9 b 412.1 ab 428.0 b 387.2 b 315.0 b 255.8 b Nitamin Nfusion (50% N) + Urea (50% N) blend 2 297.0 363.0 a 380.2 a 394.0 b 401.4 bc 522.3 b 477.0 cd 431.7 d 364.8 c 354.8 d 355.4 d 326.3 d 280.6 c 232.7 cd Nitamin Infusion (50% N) + Urea (50% N) blend 4 271.2 327.6 abc 406.1 a 474.8 a 448.4 ab 649.8 a 575.1 ab 500.4 b 409.5 b 371.8 bcd 426.0 b 374.1 bc 316.3 b 251.6 b Polyon (43-0-0) 2 285.3 309.6 bcd 304.6 b 311.8 c 341.8 d 472.0 b 471.1 d 470.3 c 406.5 b 405.9 abc 395.1 bc 365.8 bc 319.8 b 255.9 b Polyon (43-0-0) 4 275.5 301.0 cd 302.6 b 309.3 c 355.0 cd 513.1 b 529.7 bc 546.4 a 446.5 a 449.3 a 497.9 a 442.8 a 371.3 a 287.7 a 0 266.2 277.4 d 258.4 c 248.9 d 234.0 e 281.6 c 301.2 e 320.8 e 306.2 d 286.6 e 291.2 e 281.1 e 250.0 d 218.0 d *Chlorophyll index 1-999; 999 = high chlorophyll content. **Values within the same column with the same letter are not significantly different LSD P = 0.05. Figure 1. Effect of different slow-release fertilizers on chlorophyll content of Famous Kentucky bluegrass mowed at fairway height. Chlorophyll index (1-999) 700 600 500 400 300 200 3

Table 2. Effect of different slow-release fertilizers on clipping dry weight of' Famous' Kentucky bluegrass mowed at fairway height. Clipping dry weight (g/1 ft 2 ) Nitamin Nfusion (25-0-0) 1:1 dilution 2 1.04 0.68 bcd* 1.06 b 1.01 bc 1.34 bc 1.05 c 0.99 b 0.66 b 0.43 de 1.16 cd 1.19 c 1.20 bc 0.69 cd Nitamin Nfusion (25-0-0) 1:1 dilution 4 1.13 0.92 ab 1.68 a 1.87 a 3.23 a 2.16 a 1.64 a 1.00 ab 0.92 ab 2.06 a 1.71 a 1.45 ab 0.86 abc Nitamin Nfusion (50% N) + Urea (50% N) blend 2 0.96 0.79 abc 1.11 b 1.10 b 1.64 b 1.14 c 1.16 b 0.79 ab 0.54 cd 1.27 bc 1.17 cd 1.17 bc 0.71 bcd Nitamin Nfusion (50% N) + Urea (50% N) blend 4 1.15 1.09 a 1.87 a 1.93 a 3.40 a 1.90 ab 1.79 a 1.05 a 0.96 a 1.70 ab 1.52 abc 1.44 ab 0.88 ab Polyon (43-0-0) 2 0.70 0.47 cd 0.63 c 0.67 cd 1.05 bc 0.96 c 1.12 b 0.81 ab 0.71 bc 1.35 bc 1.23 bc 1.24 b 0.60 de Polyon (43-0-0) 4 1.14 0.69 bcd 0.88 bc 1.02 bc 1.70 b 1.66 b 1.74 a 1.00 ab 1.00 a 1.62 abc 1.62 ab 1.68 a 0.98 a 0 0.91 0.44 d 0.52 c 0.52 d 0.38 c 0.50 d 0.55 c 0.26 c 0.29 e 0.76 d 0.78 d 0.92 c 0.44 e *Values within the same column with the same letter are not significantly different LSD P = 0.05. Figure 2. Effect of different slow-release fertilizers on clipping dry weight of' Famous' Kentucky bluegrass mowed at fairway height. Clip DW (g/1 ft 2 ) 4 3 2 1 0 3

Table 2a. Effect of different slow-release fertilizers on total clipping dry weight over the duration of the study of' Famous' Kentucky bluegrass mowed at fairway height. Total clipping Rate Dry weight Fertilizer treatment (lbs N/M) (g) Nitamin Nfusion (25-0-0) 1:1 dilution 2 12.5 c* Nitamin Nfusion (25-0-0) 1:1 dilution 4 20.6 a Nitamin Nfusion (50% N) + Urea (50% N) blend 2 13.6 c Nitamin Nfusion (50% N) + Urea (50% N) blend 4 20.7 a Polyon (43-0-0) 2 11.6 c Polyon (43-0-0) 4 16.8 b 0 7.3 d *Values within the same column with the same letter are not significantly different LSD P = 0.05.

Table 3. Effect of different slow-release fertilizers on visual color of 'Famous' Kentucky bluegrass mowed at fairway height. Visual color (rated 1-9)* Nitamin Nfusion (25-0-0) 1:1 dilution 2 5.5 5.5 c** 5.5 c 5.5 bc 6.3 c 6.5 c 5.8 c 6.0 d 5.8 c 5.3 d 5.0 d 5.0 c 5.0 cd 5.0 b Nitamin Nfusion (25-0-0) 1:1 dilution 4 5.5 6.5 ab 6.8 a 7.5 a 7.8 a 7.5 ab 6.8 b 7.0 bc 6.8 ab 6.5 b 6.5 bc 6.5 b 6.0 b 5.5 b Nitamin Nfusion (50% N) + Urea (50% N) blend 2 5.3 6.8 a 6.0 b 6.3 b 6.3 c 6.0 c 6.0 bc 6.0 d 5.8 c 5.5 cd 5.0 d 5.0 c 4.8 d 4.8 b Nitamin Nfusion (50% N) + Urea (50% N) blend 4 5.0 5.8 bc 7.0 a 7.5 a 7.5 ab 7.8 a 6.5 bc 6.5 cd 6.5 bc 6.3 bc 6.0 c 6.0 b 5.5 bc 5.0 b Polyon (43-0-0) 2 5.3 5.5 c 5.0 d 4.8 c 6.8 bc 6.8 bc 6.8 b 7.3 ab 6.8 ab 6.8 b 6.8 b 6.3 b 6.0 b 5.5 b Polyon (43-0-0) 4 5.8 5.8 bc 5.5 c 5.5 bc 7.5 ab 8.0 a 7.8 a 7.8 a 7.5 a 8.0 a 8.0 a 7.8 a 7.8 a 7.0 a 0 4.8 5.5 c 4.8 d 3.8 d 4.0 d 3.8 d 3.8 d 3.5 e 3.8 d 3.8 e 3.8 e 3.8 d 3.8 e 3.8 e *Visual color rated 1-9; 9 = dark green. **Values within the same column with the same letter are not significantly different LSD P = 0.05. Figure 3. Effect of different slow-release fertilizers on visual color of 'Famous' Kentucky bluegrass mowed at fairway height. 9 8 Visual Color (rated 1-9) 7 6 5 4 3 2 1 3

Table 4. Effect of different slow-release fertilizers on visual turfgrass quality of 'Famous' Kentucky bluegrass mowed at fairway height. Visual turfgrass quality (rated 1-9)* Nitamin Nfusion (25-0-0) 1:1 dilution 2 5.3 ab** 5.5 bc 5.3 bc 5.0 cd 5.5 b 5.8 a 6.0 b 5.8 c 5.3 d 5.0 c 5.0 b 5.0 bc 5.0 bc Nitamin Nfusion (25-0-0) 1:1 dilution 4 6.0 a 6.0 b 7.0 a 7.3 a 7.0 a 6.8 a 6.3 b 6.8 ab 6.5 b 5.8 b 5.8 b 5.5 b 5.0 bc Nitamin Nfusion (50% N) + Urea (50% N) blend 2 6.0 a 6.0 b 6.0 ab 6.0 bc 5.8 ab 5.8 a 6.0 b 5.8 c 5.5 cd 5.0 c 5.0 b 4.8 c 4.8 c Nitamin Nfusion (50% N) + Urea (50% N) blend 4 5.8 a 6.8 a 6.5 a 6.5 ab 6.3 ab 6.3 a 6.5 b 6.5 bc 6.3 bc 5.8 b 5.3 b 5.3 bc 5.0 bc Polyon (43-0-0) 2 5.3 ab 5.0 cd 4.5 cd 4.8 d 5.0 bc 5.8 a 6.3 b 6.8 ab 6.5 b 6.0 b 5.8 b 5.5 b 5.5 ab Polyon (43-0-0) 4 5.3 ab 5.3 c 4.5 cd 4.8 d 5.5 b 6.0 a 7.5 a 7.5 a 8.0 a 7.5 a 7.0 a 6.8 a 5.8 a 0 4.5 b 4.5 d 3.8 d 3.5 e 3.8 c 3.8 b 3.5 c 3.8 d 3.8 e 3.8 d 3.8 c 3.8 d 3.8 d *Visual color rated 1-9; 9 = dark green. **Values within the same column with the same letter are not significantly different LSD P = 0.05. Figure 4. Effect of different slow-release fertilizers on visual turfgrass quality of 'Famous' Kentucky bluegrass mowed at fairway height. 9 Visual quality (rated 1-9) 8 7 6 5 4 3 2 1 3