1. IDENTIFICATION OF THE SUBSTANCE/MIXTURE AND OF THE COMPANY/UNDERTAKING 1.1. PRODUCT IDENTIFICATION Chemical Type: Laboratory chemicals, substance Product Name: Fixation solution1; Ethanol (CAS-No.: 64-17-5) Dangerous substances: None 1.2. RELEVANT IDENTIFIED USES OF THE SUBSTANCE OR MIXTURE AND USES ADVISED AGAINST Use of the substance/mixture The product is intended for research, analysis and scientific education. Uses advised against None. 1.3. DETAILS OF THE SUPPLIER AND SAFETY DATA SHEET Company: BioMode 2, S.A. Address: Edificio GNRation Praça Conde de Agrolongo, nº 123 4700-312 Braga, Portugal. Telephone No.: +351 253 140 161 E-mail: info@biomode-sa.com Contact person: Laura Cerqueira 1.4. EMERGENCY TELEPHONE Emergency telephone number: 808 250 143 (PT, 24h) 2. HAZARDS IDENTIFICATION 2.1. CLASSIFICATION OF THE SUBSTANCE OR MIXTURE Classification according to Regulation (EC) No 1272/2008 [EU-GHS/CLP] Flammable liquids (Category 2) Classification according to EU Directives 67/548/EEC or 1999/45/EC: Highly flammable. 2.2 LABEL ELEMENTS Labelling according to Regulation (EC) No. 1272/2008 [CLP]: Hazard pictograms (CLP): Signal word: Hazard statements (CLP): H225 Precautionary statements (CLP): Danger Highly flammable liquid and vapor. Edition: 01 Last revision: 22-03-2016 Page 1 of 8
P210 Supplemental Hazard Statements Keep away from heat/sparks/open flames/hot surfaces. No smoking. None Labelling according to Directive 67/548/EEC or 1999/45/EC: Hazard symbols: R-phrases: R11 S-phrases: S7 S16 Highly flammable. Keep container tightly closed. Keep away from ignition sources. No smoking. 3. COMPOSITION/INFORMATION ON INGREDIENTS 3.1. SUBSTANCES NAME PRODUCT IDENTIFIER % (V/V) CLASSIFICATION ACCORDING TO REGULATION (EC) NO. 1272/2008 ETHANOL 64-17-5 (CAS No) 50 Flammable liquids (Category 2) 4. FIRST AID MEASURES 4.1. DESCRIPTION OF FIRST AID MEASURES General description Consult a physician show this safety data sheet to the doctor in attendance. After inhalation If breathed in, move person into fresh air. If not breathing, give artificial respiration. Consult a physician. In case of skin contact Wash off with soap and plenty of water. Consult a physician. In case of eye contact Rinse thoroughly with plenty of water for at least 15 minutes and consult a physician. After ingestion Never give anything by mouth to an unconscious person. Rinse mouth with water. Consult a physician. 4.2. INDICATION OF ANY IMMEDIATE MEDICAL ATTENTION AND SPECIAL TREATMENT NEEDED 5. FIREFIGHTING MEASURES 5.1. EXTINGUISHING MEDIA Use water spray, alcohol-resistant foam, dry chemical or carbon dioxide. Edition: 01 Last revision: 22-03-2016 Page 2 of 8
5.2. SPECIAL HAZARDS ARISING FROM THE SUBSTANCE OR MIXTURE Carbon oxides. 5.3. ADVICE FOR FIGHTERS Wear self-contained breathing apparatus for firefighting if necessary. 5.4. ADDITIONAL INFORMATION Use water spray to cool unopened containers. 6. ACCIDENTAL RELEASE MEASURES 6.1. PERSONAL PRECAUTIONS, PROTECTIVE EQUIPMENT AND EMERGENCY PROCEDURES Use personal protective equipment. Avoid breathing vapors, mist or gas. Ensure adequate ventilation. Evacuate personnel to safe areas. Remove all sources of ignition. Beware of vapours accumulating to form explosive concentrations. Vapours can accumulate in low areas. 6.2. ENVIRONMENTAL PRECAUTIONS Prevent further leakage or spillage if safe to do so. Do not let product enter drains. 6.3. METHODS AND MATERIAL FOR CONTAINMENT AND CLEANING UP Contain spillage, and then collect with an electrically protected vacuum cleaner or by wet-brushing and place in container for disposal according to local regulations (see section 13). 6.4. REFERENCE TO OTHER SECTIONS For disposal see section 13. 7. HANDLING AND STORAGE 7.1. PRECAUTIONS FOR SAFE HANDLING Advice on safe handling Avoid exposure. Avoid contact with skin and eyes. Avoid inhalation of vapor or mist. Advice on protection against fire and explosion Keep away from sources of ignition. No smoking. Take measures to prevent the build-up of electrostatic charge. 7.2. CONDITIONS FOR SAFE STORAGE, INCLUDING ANY INCOMPATIBILITIES Advice on storage compatibility Store in cool place. Keep container tightly closed in a dry and well-ventilated place. Containers which are opened must be carefully resealed and kept upright to prevent leakage. 8. EXPOSURE CONTROLS/PERSONAL PROTECTION Edition: 01 Last revision: 22-03-2016 Page 3 of 8
8.1. CONTROL PARAMETERS Components with work place control parameters No available data. Occupational exposure controls Refer to chapter 7. No further action is necessary. 8.2. EXPOSURE CONTROLS Respiratory protection Where risk assessment shows air-purifying respirators are appropriate use a full-face particle respirator type N100 (US) or type P3 (EN 143) respirator cartridges as a backup to engineering controls. If the respirator is the sole means of protection, use a full-face supplied air respirator. Use respirators and components tested and approved under appropriate government standards such as NIOSH (US) or CEN (EU). Eye protection Face shield and safety glasses. Use equipment for eye protection tested and approved under appropriate government standards such as NIOSH (US) or EN166 (EU). Skin protection Handle with gloves. Gloves must be inspected prior to use. Use proper glove removal technique (without touching glove's outer surface) to avoid skin contact with this product. Dispose of contaminated gloves after use in accordance with applicable laws and good laboratory practices. Wash and dry hands. The selected protective gloves have to satisfy the specifications of EU Directive 89/686/EEC and the standard EN 374 derived from it. Immersion protection: Material: Nitrile rubber. Minimum layer thickness: 0.11 mm. Break through time: > 480 min. Material tested: Dermatril (Aldrich Z677272, Size M). Splash protection: Material: Nitrile rubber. Minimum layer thickness: 0.11 mm. Break through time: > 30 min. Material tested: Dermatril (Aldrich Z677272, Size M). Data source: KCL GmbH, D-36124 Eichenzell, phone +49 (0)6659 873000, e-mail sales@kcl.de. Test method: EN374. Edition: 01 Last revision: 22-03-2016 Page 4 of 8
If used in solution, or mixed with other substances, and under conditions which differ from EN 374, contact the supplier of the CE approved gloves. This recommendation is advisory only and must be evaluated by an Industrial Hygienist familiar with the specific situation of anticipated use by our customers. It should not be construed as offering an approval for any specific use scenario. Body protection No data available Environmental exposure controls This material and its container must be disposed of in a safe way. 9. PHYSICAL AND CHEMICAL PROPERTIES 9.1. INFORMATION ON BASIC PHYSICAL AND CHEMICAL PROPERTIES Appearance Liquid, clear Color: colorless Odor ph Melting point/freezing point Melting point/range: -114 C - lit. Initial boiling point and boiling range 78.3 C Flash point 14.0 C - closed cup Evaporation rate Flammability Upper/lower flammability limits Upper explosion limit: 19 %(V) Lower explosion limit: 3.3 %(V) Vapor pressure 59.5 hpa at 20.0 C Vapor density Relative density 0.789 g/ml at 20 C0.789 g/ml at 25 C Solubility completely soluble Auto-ignition temperature 363.0 C Partition coefficient: n-octanol/water 10. STABILITY AND REACTIVITY 10.1. CHEMICAL STABILITY 10.2. POSSIBILITY OF HAZARDOUS REACTIONS 10.3. REACTIVITY 10.4. CONDITIONS TO AVOID Heat, flames and sparks. Extremes of temperature and direct sunlight. 10.5. INCOMPATIBLE MATERIALS Edition: 01 Last revision: 22-03-2016 Page 5 of 8
Alkali metals, Ammonia, Oxidizing agents, Peroxides. SAFETY DATA SHEET 11. TOXICOLOGICAL INFORMATION 11.1. INFORMATION ON TOXICOLOGICAL EFFECTS Acute toxicity Skin corrosion/irritation Eye irritation Respiratory or skin sensitization Germ cell mutagenicity Carcinogenicity Reproductive toxicity Specific target organ toxicity - single exposure Specific target organ toxicity - repeated exposure Aspiration hazard LD50 Oral - rat - 7,800 mg/kg LD50 Inhalation - rat - 10,000 ppm 10 h LD50 Dermal - rabbit - 8,000 mg/kg Irritating to Skin 24 h Mild eye irritation - 24 h Draize test IARC: No component of this product present at levels greater than or equal to 0.1% is identified as probable, possible or confirmed human carcinogen by IARC. Reproductive toxicity - Human - female - Oral Effects on Newborn: Apgar score (human only). Effects on Newborn: Other neonatal measures or effects. Effects on Newborn: Drug dependence. Inhalation - May cause respiratory irritation. 12. ECOLOGICAL INFORMATION 12.1. TOXICITY 12.2. PERSISTENCE AND DEGRADABILITY 12.3. BIOACCUMULATIVE POTENTIAL 12.4. MOBILITY IN SOIL 12.5. RESULTS OF PBT AND VPVB ASSESSMENT 12.6. OTHER ADVERSE EFFECTS 13. DISPOSAL CONSIDERATIONS 13.1. WASTE TREATMENT METHODS Advice on disposal Edition: 01 Last revision: 22-03-2016 Page 6 of 8
Waste disposal according to official state regulations. Consult the local waste disposal expert about waste disposal. Waste disposal number for residues/unused products 160506 WASTES NOT OTHERWISE SPECIFIED IN THE LIST; gases in pressure containers and discarded chemicals; laboratory chemicals, consisting of or containing dangerous substances, including mixtures of laboratory chemicals Classified as hazardous waste. Waste disposal number for used products 160506 WASTES NOT OTHERWISE SPECIFIED IN THE LIST; gases in pressure containers and discarded chemicals; laboratory chemicals, consisting of or containing dangerous substances, including mixtures of laboratory chemicals Classified as hazardous waste. Waste disposal number of contaminated packaging 150110 WASTE PACKAGING; ABSORBENTS, WIPING CLOTHS, FILTER MATERIALS AND PROTECTIVE CLOTHING NOT OTHERWISE SPECIFIED; packaging (including separately collected municipal packaging waste); packaging containing residues of or contaminated by dangerous substances Classified as hazardous waste. Contaminated packaging Cleaned containers may be recycled. 14. TRANSPORT INFORMATION 14.1. UN NUMBER ADR/RID: 1170 IMDG: 1170 IATA: 1170 14.2. UN PROPER SHIPPING NAME ADR/RID: IMDG: IATA: ETHANOL ETHANOL Ethanol 14.3. TRANSPORT HAZARD CLASS(ES) ADR/RID: 3 IMDG: 3 IATA: 3 14.4. PACKAGING GROUP ADR/RID: II IMDG: II IATA: II 14.5. ENVIRONMENTAL HAZARDS ADR/RID: no IMDG Marine Pollutant: no IATA: no 15. REGULATORY INFORMATION This safety datasheet complies with the requirements of Regulation (EC) No. 1907/2006. 15.1. SAFETY, HEALTH AND ENVIRONMENTAL REGULATIONS/LEGISLATIONS SPECIFIC FOR THE SUBSTANCE OR MIXTURE Edition: 01 Last revision: 22-03-2016 Page 7 of 8
16. OTHER INFORMATION 16.1. CHANGES First edition. 16.2. FULL TEXT OF R-PHRASES REFERRED TO UNDER THE SECTION 2 AND 3 R11 Highly flammable. 16.3. FULL TEXT OF H-STATEMENTS REFERRED TO UNDER THE SECTION 2 AND 3 H225 Highly flammable liquid. Edition: 01 Last revision: 22-03-2016 Page 8 of 8