Fish Assessment for Sanctuary Pond October 10, 2005

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83 Ambrogio Dr, Suite K Gurnee, IL 60030 8847-244-6662 847-244-0261 fax www.lakesmanagement.com Fish Assessment for Sanctuary Pond October 10, 2005 Prepared For: Dr. Mike Sands Executive Director Liberty Prairie Foundation 847-548-4062X10 mikesands@prairiecrossing.com Prepared By: Ms. Ingrid West Mr. Christopher Ryan 83 Ambrogio Drive, Suite K Gurnee, Illinois www.lakesmanagement.com Integrated Lakes Management S.Share1/Projects and Proposals/Fishery/sanctuary

83 Ambrogio Dr, Suite K Gurnee, IL 60030 8847-244-6662 847-244-0261 fax www.lakesmanagement.com Fish Assessment for Sanctuary Pond October 10, 2005 Introduction Integrated Lakes Management staff completed a quantitative fisheries survey on September 8th to assess the relative abundance of fish in Sanctuary Pond. This was in addition to the sampling done earlier in the season on May 11 th. Sanctuary Pond has been the rearing pond for a stocking project for several endangered and threatened fish species. The species that are in the pond include banded killifish, Iowa darters, blackchin and blacknose shiners and some incidental pugnose shiners that were inadvertently stocked. Over the years the fish have been consistently monitored and this study is a continuation of that monitoring program. Methodology Thirty foot seine hauls were done in various locations around the pond these were done as ten and thirty foot pulls for a total of 6 seine pulls. A casting net was used for the open water areas of the pond. (This method was not as effective as hoped due to the thick coontail beds across the entire pond floor.) The general water quality conditions were assessed on September 21 nd 2005. Integrated Lakes Management S.Share1/Projects and Proposals/Fishery/sanctuary

Results Total Collection/All Gear Types Total % Blackchin Shiner Notropis heterodon 185 43 Blacknose Shiner Notropis heterolepis 93 22 Banded Killifish Fundulus diaphanus 151 35 Iowa Darter Etheostoma exile 0 0 All fish collected 1,987 ILM attempted to use several sampling strategies in order to collect a variety of species in Sanctuary Pond. Sanctuary pond has a very healthy population of the three lotic fish populations; those being the banded killifish, and the blacknose and blackchin shiner. Every seine pull that was made had such large quantities of these fish that only sub-samples of each species were measured. The rest of the fish were mass counted and a percent of the total population is inferred. There was good distribution of sizes in the fish collected. Tiny fish were observed but were not identified to species and many escaped through the net mesh. The fact that young of the year were observed and collected shows that there is adequate reproduction. All the fish collected were in excellent condition and some appeared to be currently spawning. In comparing the May and September data there is definite annual recruitment to each of the three populations. We also saw the population size and condition factor increase for all three species. This indicates that these populations are doing well. However, due to the soft nature of the substrate and the abundance of vegetation it was very hard to effectively collect the Iowa darter. We do know that Iowa darters are present in the pond since several were collected during three separate collections earlier in the year. The first collection was for our study the second was for the University of Illinois at Chicago for their genetic variability study and the third collection where Iowa darters were collected was for the Brookfield Zoo stocking project. Unfortunately even during these collections few individuals were collected. It is not known if the lack of numbers is due to the inability to effectively collect this bottom species in a pond that is so heavily vegetated or if the population numbers are few due to habitat unsuitability. Further investigation specific for Iowa darters is warranted to make an accurate determination. The primary predator in the pond is the frog population, principally the bullfrogs. Another predator for the minnows are the predaceous diving beetles and other macroinvertebrates that are piscivores. These along with birds are the only known predation mortality to these fish. Integrated Lakes Management S.Share1/Projects and Proposals/Fishery/sanctuary

Recommendations: Continue to monitor the populations of all species in order to verify population stability. Use multiple sampling strategies to diversify catch, especially to sample the Iowa Darter. Consider monitoring the bullfrog population to determine if this population is growing. Since bullfrogs are a nuisance species it will be up to the Homeowner Association to determine whether they wish to treat this species similar to the way sparrows are monitored as a pest for bluebird nesting boxes. Plant management options needs to be considered in conjunction with the habitat requirements of new native species that are appropriate for this pond. o Encourage removal of excessive coontail, Eurasian water milfoil, and possibly planting other native submergent plant species in order to compete with these two nuisance plants. Coontail is not considered as beneficial as other native plant species. Coontail grows into extensive dense weed beds and does not contribute as positive a water quality benefit to the water column as other native plants. Another important monitoring consideration. Earlier in the season Eurasian water milfoil weevils were noted in the pond. It may be important to monitor the weevil population and predation on these weevils by the minnow species. Since we are promoting a fish population that is not balanced by predation these fish could be a primary predator on the weevils. Integrated Lakes Management S.Share1/Projects and Proposals/Fishery/sanctuary

Sanctuary Pond Water Chemistry Data September 21, 2005 Air Temp 80 Degrees Precipitation 0.3 (within 2 previous days) Total Depth Site 1 Site 2 Secchi Depth 4 4 10 ph 8.5 Alkalinity 103 Chloride 180 IEPA color chart 13 13 Total Depth 7 2 7 9 Water Chemistry at Deep hole Site 1 Site 2 Depth Temp Cond DO PH Temp Cond DO PH Surface 21.7 823 6.0 8.6 21.6 786 9.3 9.4 1 21.7 826 56 8.6 21.9 782 9.5 9.5 2 21.6 826 5.6 8.6 20.9 785 7.8 9.4 3 21.2 823 5.4 8.6 20.4 788 7.2 9.4 4 20.7 820 4.4 8.6 20.0 795 2.9 9.2 5 20.4 850 1.5 8.2 19.6 818 1.2 8.7 6 20.0 1020 0.7 7.5 19.1 981 0.5 7.9 7 19.3 1147 0.4 6.9 18.6 1150 0.4 7.3 Bottom Integrated Lakes Management S.Share1/Projects and Proposals/Fishery/sanctuary

Blackchin Shiner Age/Length Data ( Becker) Compared May and September Distribution. Blackchin shiner Age/Length Data (Becker) Becker s Age Class Size Range ( mm) Sanctuary Pond Sanctuary Pond % Total 0 M 25-35 39 38% 0 S 7 17% I M 37-53 54 53% I S 25 61% II M 48-62 9 9% II S 7 17% III M 61-67 0 NA III S 2 5% Becker collection was done in August Blacknose Shiner Age/Length Data ( Becker) Becker s Age Size Range Sanctuary Pond Sanctuary Pond % Class ( mm) 0 M 27-35 0 0% 0 S 0 0% M 35-45 21 77% S 6 15% 1 M 45-55 4 14% 1 S 14 34% M 56-70 2 7% S 21 51% Banded Killifish Age/Length Data ( Becker) Becker s Age Size Range Sanctuary Pond Sanctuary Pond % Class ( mm) 0 M 25-40 44 50% 0 S 4 10% I M 45-65 29 33% I S 32 78% II M 60-69 14 16% II S 4 10% III M 70 1 <1% III S 1 2% Becker collections were done in August Integrated Lakes Management S.Share1/Projects and Proposals/Fishery/sanctuary

Fish Assessment for Sanctuary Pond September, 2005 Black Chin Blacknose Killifish # CM # CM # CM 1 2.10 1 5.50 1 6.30 2 3.30 2 4.80 2 5.00 3 5.10 3 4.50 3 5.40 4 3.80 4 5.80 4 4.30 5 3.80 5 5.40 5 6.00 6 4.60 6 6.20 6 4.30 7 4.20 7 5.30 7 4.40 8 3.10 8 5.90 8 4.00 9 4.50 9 5.10 9 5.00 10 3.60 10 4.00 10 5.00 11 3.00 11 5.20 11 3.70 12 3.00 12 5.40 12 4.10 13 3.80 13 5.00 13 4.20 14 3.10 14 4.30 14 4.50 15 3.80 15 5.00 15 4.70 16 2.60 16 4.60 16 3.60 17 3.20 17 5.20 17 4.60 18 2.90 18 4.50 18 4.10 19 4.10 19 4.30 19 4.70 20 3.60 20 5.20 20 4.30 21 3.20 21 6.30 21 4.30 22 3.70 22 5.20 22 4.50 23 3.50 23 4.50 23 5.00 24 3.70 24 3.80 24 5.90 25 3.30 25 5.60 25 4.70 26 3.90 26 4.30 26 4.90 27 3.50 27 4.50 27 4.70 28 4.80 28 3.70 28 4.60 29 4.00 29 4.00 29 4.70 30 3.70 30 5.50 30 5.70 31 3.00 31 6.10 31 5.00 32 3.10 32 3.70 32 4.80 33 4.80 33 4.80 33 4.90 34 4.50 34 6.30 34 4.70 35 5.10 35 3.90 35 4.30 36 3.90 36 5.00 36 4.60 37 3.10 37 5.80 37 4.60 38 3.00 38 5.30 38 4.80 39 3.40 39 4.90 39 4.80 40 3.80 40 5.10 40 3.90 41 3.50 41 6.20 41 5.00 Total # fish sampled 185 93 151 1,987

INTEGRATED LAKES MANAGEMENT Blackchin Shiner Distribution For September 2005 Fish Collection Form 23-Sep-05 10:00 AM Crew: I. West Locality: Prairie Crossing C. Ryan Site Name Sanctuary Pond Seine Blackchin Shiner Length Frequencies Numbers 30 25 20 15 10 5 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 Size in cm Mean 3.65122 Standard Error 0.104247 Median 3.6 Mode 3.8 Standard Deviation 0.667504 Sample Variance 0.445561 Kurtosis 0.102619 Skewness 0.366458 Range 3 Minimum 2.1 Maximum 5.1 Sum 149.7 Count 41

INTEGRATED LAKES MANAGEMENT Blacknose Shiner Distribution For September 2005 Fish Collection Form Dates: 23-Sep-05 Crew: I. West Locality: Prairie Crossing Time: 10:00 AM C. Ryan Site Name: Sanctuary Pond Gear: Seine Numbers 20 15 10 5 0 Blacknose Shiner Length Frequencies Sienes 1 3 5 7 9 11 13 15 17 19 21 23 25 27 Size in cm Mean 5.017073171 Standard Error 0.115394469 Median 5.1 Mode 4.5 Standard Deviation 0.738885119 Sample Variance 0.54595122 Kurtosis -0.765682002 Skewness -0.036859042 Range 2.6 Minimum 3.7 Maximum 6.3 Sum 205.7 Count 41

INTEGRATED LAKES MANAGEMENT Banded Killifish Distribution For September 2005 Fish Collection Form Dates: 23-Sep-05 Crew: I. West Locality: Prairie Crossing Time: 10:00 AM C. Ryan Site Name Sanctuary Pond Gear: Seine Banded Killifish Length Frequencies 35 30 25 20 15 10 5 0 1 3 5 7 9 11 13 15 17 19 21 23 25 Size in cm Numbers Mean 4.697561 Standard Error 0.089544 Median 4.7 Mode 5 Standard Deviatio 0.573362 Sample Variance 0.328744 Kurtosis 1.170381 Skewness 0.771247 Range 2.7 Minimum 3.6 Maximum 6.3 Sum 192.6 Count 41

INTEGRATED LAKES MANAGEMENT Iowa Darter Distribution For September 2005 Fish Collection Form Dates: 23-Sep-05 Crew: Chris Ryan Locality: Prairie Crossing Time: 10:00 AM Ingrid West Site Name Sanctuary Pond Gear: seine none

Field Notes for Fish Collection for Sanctuary Pond for May 11 th, 2005. ILM staff members included Sandy Kubillus, Jim Bland, and Pat Bland. Three seine hauls were made using a 20 ft. X 6ft. common sense seine with ¼ hexagonal mesh. Seine hauls were done at the southwest corner of the pond which has historically been the locality for monitoring the fish populations. All seine hauls were done from 4.5 ft. depth to the shoreline which was inches deep. Fish are collected in the seine and then held in a 3 ft. diameter open wash tub in about 6 inches of water. A small aquarium net is used to move fish from the open wash tub to a measuring board. Efforts are made to try to randomize our selection of fish so that no size or species bias is involved in the subsample. We encountered several individuals which appeared to have fin rot, parasites, and a sunken stomach. We kept these individuals for purposes of documenting potential pathologies associated with these different species. Data on the collections was converted to length frequency curves. Half centimeter increments were used to define the length categories. The tables below compare length and age as described in Becker s Fishes of Wisconsin with the length /frequency categories obtained as part of our May 11 th collections. Life history data compiled by Burr et.al. identify blackchin shiners ( Notropis heterodon) and blacknose shiners ( Notropis heterolepis) as having multiple spawning cycles across the season. Blackchin Shiner Age/Length Data ( Becker) Compared To May 11 th Distribution. Becker s Age Class Size Range Sanctuary Pond Sanctuary Pond ( mm) % 25-35 39 38% I 37-53 54 53% II 48-62 9 9% III 61-67 0 NA Becker collection was done in August The differences in size range and age group may be attributable to the time of year that the collections were undertaken.

Blacknose Shiner Age/Length Data ( Becker) Compared to May 11 th Collection Becker s Age Class Size Range ( mm) Sanctuary Pond Sanctuary Pond % 0 27-35 0 35-45 21 77 1 45-55 4 14 56-70 2 7 Blacknose shiners are only known to have a two year life cycle. Multiple spawning events occur however across a season. The frequency distribution for both shiners may be a reflection of multiple spawning events across a season. Banded Killifish Age/Length Data ( Becker) Compared to May 11 th Collection Becker s Age Class Size Range ( mm) Sanctuary Pond Sanctuary Pond % 0 25-40 44 50 I 45-65 29 33 II 60-69 14 16 III 70 1 <1 Becker collections were done in August The size range doesn t compare well with data which occurs in Becker. ILM suggests that a series be collected for purposes of aging the population. Collections done in August would probably be most suitable. Morphological characters suitable for aging need to be determined for each species. ILM encountered a single Banded killifish female that was clearly pregnant. Thus spawning in May is quite likely. We collected a single male Iowa darter that appeared to display spawning coloration. The darter was 56 mm in size. It had a sunken stomach that was suggestive of intestinal parasites. The specimen was kept for future examination.

Fish Assessment at Sanctuary Pond May 11th 2005 Species Banded killifish Blackchin shiner Blacknose shiner Pugnose shiner Iowa darter # Size: cm Size: cm Size: cm Size: cm Size: cm 1 3.9 4.5 3.7 5.6 2 5.7 4.7 3.2 3 5.7 4 3.2 4 4.7 3.2 3.2 5 2.7 3.2 3.1 6 3.2 2.2 3.3 7 3.4 2.5 4 8 4.7 2.5 4 9 4.5 3 4 10 4.3 3.5 3.5 11 3.9 4.2 4.1 12 3.2 2.8 3.6 13 3 3 3.2 14 3.6 2.7 4 15 3.5 3 4 16 3 3 3.8 17 3 2.7 3.5 18 3 3 5.5 19 6.5 3.2 6.7 20 5.5 3.2 4 21 3.3 2.7 5 22 4.3 3 4.5 23 3.2 2.3 3.7 24 3.4 4.6 3.5 25 4 5 4 26 4.1 3.2 6.5 27 2.5 4.6 3.3 28 2.7 2.8 29 3.2 2.8 30 6 2.5 31 5.3 5.4 32 5.5 4.5 33 5 3.7 34 3.9 3 35 7 2.7 36 6.5 3.2 37 5.5 3.3 38 3.4 3 39 6 3 40 5 2.6 41 6 2.7 42 5.9 2.9 43 6 3.1 44 5 3.1 45 2.5 3.7 46 6.5 4.5 47 5.1 4.2 48 3.5 2.7 49 6 2.3 50 3.8 2.3 51 6 2.7 52 3 5 53 6.1 3.5 54 5 3.5 55 3.1 3.2 56 5.5 2.5 57 4 2.2 58 3.5 4.2 59 3.5 3 60 3.2 2.7 61 5.5 3.1 62 6.5 4 63 4.5 3.5 64 3.8 3.4 65 2.7 2.5 66 3.3 3.6 67 2.7 4 68 4.2 3 69 4.3 2.5 70 3.2 3.2 71 3.7 4.7 72 3.1 3.5 73 2.8 3.2 74 3.7 5 75 3.2 3.3 76 3.2 4 77 3.7 4 78 3.4 4.2 79 2.7 4.3 80 3.2 81 3.5 82 3.7 83 3.2 84 3.9 85 4.1 86 3.5 87 2.5 88 3.5 89 3 90 3.6 91 4.3 92 4 93 4.4 94 4 95 3.5 96 3.5 97 2.5 98 4.9 99 3.5 100 3.9 101 3.4 102 2.7 Count 79 102 27 0 1 Avg. 4.23 3.40 4.00 0.00 5.60

INTEGRATED LAKES MANAGEMENT Blackchin Shiner Distribution For May 2005 Fish Collection Form Prairie Crossing Dates: 11-May-05 Crew: Pat Bland Sanctuary Pond Time: 10:00 AM Jim Bland Sandy Kubillus Gear: seine Blackchin Shiner Distribution at Sanctuary Pond in Grayslake 35 30 25 20 15 10 5 0 2.0 3.0 4.0 5.0 6.0 Length (cm) 7 Mean 3.394059 Standard Error 0.072968 Median 3.2 Mode 3 Standard Deviation 0.733324 Sample Variance 0.537764 Kurtosis -0.28459 Skewness 0.601749 Range 3.2 Minimum 2.2 Maximum 5.4 Sum 342.8 Count 101 Confidence Level(9 0.144767

INTEGRATED LAKES MANAGEMENT Blacknose Shiner Distribution For May 2005 Fish Collection Form Dates: 11-May-05 Crew: Pat Bland Locality: Prairie Crossing Time: 10:00 AM Jim Bland Site Name Sanctuary Pond Sandy Kubillus Gear: seine Mean 4.01538462 Standard Error 0.18577839 Median 3.9 Mode 4 Standard Deviation 0.94728763 Sample Variance 0.89735385 Kurtosis 2.86707497 Skewness 1.77586923 Range 3.6 Minimum 3.1 Maximum 6.7 Sum 104.4 Count 26 Confidence Level(95 0.38261748 12 11 10 9 8 7 6 5 4 3 2 1 0 Distribution for Blacknose Shiners at Sanctuary Pond in Grayslake Illinois 2 3 4 5 6 Length( cm) 7

INTEGRATED LAKES MANAGEMENT Banded Killi Distribution For May 2005 Fish Collection Form Dates: 11-May-05 Crew: Pat Bland Locality: Prairie Crossing Time: 10:00 AM Jim Bland Site Name: Sanctuary Pond Sandy Kubillus Gear: seine Distribution for Banded Killifish at Sanctuary Pond in Grayslake, Illinois 22 20 18 16 14 12 10 8 6 4 2 0 2.5 3.5 4.5 5.5 6.5 Length (cm) Mean 4.234615 Standard Error 0.139892 Median 3.85 Mode 3.2 Standard Deviation 1.235495 Sample Variance 1.526449 Kurtosis -1.03215 Skewness 0.515293 Range 4.5 Minimum 2.5 Maximum 7 Sum 330.3 Count 78 Confidence Level(95.0%) 0.278561