室内飼育下におけるワタリイシガニの幼生飼育の最適塩分 誌名 水産増殖 = The aquiculture ISSN 3714217 著者 巻 / 号 諸喜田, 茂充藤田, 喜久 Islam, Md.S. 48 巻 4 号 掲載ページ p. 623-63 発行年月 2 年 12 月 農林水産省農林水産技術会議事務局筑波事務所 Tsukuba Office, Agriculture, Forestry and Fisheries Research Council Secretariat
SUISANZOSHOKU 4623632 Effect of Salinity on the Lar val Development of the Swimming Crab Charybdis natator Herbst (Crustacea: Decapoda: Brachyura: Portunidae) Reared in the Laborator y Md. Sirajul ISLAM, Shigemitsu SHOKITA, and Yoshihisa FUJITA Accepted October 5, 2 Abstract: Zoea larvae of the swimming crab Charybdis natator (Herbst) were reared in the laboratory under eight salinity levels. Zoea larvae survived to megalopa stage only at 2 to 35 salinity. At the lower salinities,, 5 and 1, zoeae I to VI did not moult to the subsequent stage within 12 hours of exposure. Total duration required for zoea I to moult into megalopa stage at 2, 25, 3 and 35 was 44, 38, 28 and 36 days, respectively. Each zoeal stage developed most successfully in 3 at 24, followed by 35 and 25. Significant difference (P.5) in survival rate was found among the salinities tested. The survival rates of zoeae I to VI at 25 and 3 was significantly higher (P.5) than at lower salinities. Zoea larvae at 3 showed the highest survival rate, and this salinity was found suitable for the development of C. natator larvae. Key words: Charybdis natator; zoea larvae; salinity levels; development duration The family Portunidae comprises one of the most dominant groups of marine crabs that support important fisheries in the Indo-West Pacific 1). The large swimming crab, Charybdis natator (Herbst), is mostly found in the Indian and the Western Pacific Ocea 2), where it is caught commercially and contributes to the crab fisheries in India and Australia 3). This species is widely distributed in the Indo-West Pacific and is found from East Africa, Madagascar and the Red Sea, India, Thailand, Malaysia, Singapore, Indonesia, the Philippines, Taiwan, China, Japan and Australia 2,4-7). C. natator was first found near Malaysia and Singapore 8,9). Studies on the life history of the portunid crabs have been carried out for the last 1 years, although larval development is poorly understood 1,1). The larval development of several species of the genus Charybdis has been previously described 1,11-18). Studies on salinity tolerance of the brachyuran crabs were originally reported in 1944 for temperate species, and more recently for the tropical and subtropical species 19,2). Many environmental factors affect larval development of crabs, including salinity, which has various physiological and ecological effects 21-23). Several studies have focused on the effects of various salinity levels on larval development of different crab species, e.g. Thalamita crenata Latreille 19) ; Eriocheir sineis H. Milne Edwards 21) ; Necora puber Linnaeus 22) ; Ranina ranina Linnaeus 23) ; Armases miersii Rathbun 24,25) ; Sesarma angustipes Dana 26) ; Callinectes sapidus Rathbun 27,28) ; Scylla serrata Forskal 29,3) ; A. ricordi H. Milne Edwards 31) ; A. roberti H. Milne Edwards 31) ; Chasmagnathus convexus De Haan 32) ; Cancer irroratus Say 33) ; Helice leachi Hess 34) ; H. formoseis Rathbun 34) ; and S. curacaoee De Man 35). However, the effects of salinity on larval development of C. natator have not been previously documented. Because of the importance of this species for fisheries, we examined the influence of salinity on larval survival and identified the salinity level most suitable for C. natator zoeal development. Graduate School of Engineering and Science, Department of Chemistry, Biology and Marine Science, University of the Ryukyus, 1 Senbaru, Nishihara-cho, Okinawa 93-213, Japan.
M. S. Islam, S. Shokita, and Y. Fujita Materials and Methods An ovigerous female of Charybdis natator (76.5 mm carapace length and 112.6 mm carapace width) was captured by gill net from the Pacific coast of Chinen Fisheries Port, Okinawa Island, Japan, on 14 April 1999. The female was brought to the laboratory of the Department of Chemistry, Biology and Marine Science, University of the Ryukyus, Okinawa and reared in a plastic container (32 18 22 cm 3 ) with seawater (311salinity) and moderate aeration. The female was fed shrimp (Penaeus monodon Fabricius) and short-necked clam (Ruditapes philippinarum Adams and Reeve) flesh. Seawater was changed daily until the eggs hatched. Water temperature in the container ranged from 21.3 to 24.7 (mean 23..1). Hatching occurred in the morning of 17 April 1999. Hatched larvae were reared under the same conditio as indicated for the ovigerous female. Within two days after hatching, the mostly photo positive and active zoeae were selected for culture and larval stages were identified under a binocular stereomicroscope (Nikon SMZ-1). A 5-ml glass pipette was used to retrieve the active zoeae from the rearing container and place them into one liter plastic bowls containing the test solutio. This procedure was repeated for all stages until each container held 2 individuals. The larvae were subjected to eight salinity levels (, 5, 1, 15, 2, 25, 3 and 35), measured by an asactometer (Atago Hand Refractometer, S/Mill-E, -1, Japan) to the nearest 1. The above salinities were obtained by diluting filtered seawater with dechlorinated tap water. Water temperature in the plastic test containers (32 18 22 cm 3 ) was measured daily with a hand-held thermometer to the nearest.1 and ranged from 22.5 to 25.5 (mean 24..1) throughout the experimental period. Half of the aerated test water in each container was replaced daily. Newly hatched nauplii of the brine shrimp Artemia salina (Linnaeus) were added daily to each bowl. The megalopa was fed finely chopped flesh of the short-necked clam and newly hatched brine shrimp. Moulting and survival were checked for each zoeal stage. Dead zoeae were preserved in 5% ethylene glycol for identification of stages. Experiments were terminated when all the zoeae had moulted to the megalopa stage or died. Mortality and duration of zoeal development at each salinity level were analyzed using one way ANOVA followed by Dunnett s Multiple Comparison test. Results Table 1 represents the development duration and mortalities (%) for different stages at different salinity levels. Development duration at different salinity levels varied among the different Duration and mortality of different zoeal stages of Charybdis natator (Herbst) at various salinity levels 1 Salinity Developmental stage () 15 2 25 3 35 zoea I 7.25.5 (45, N=9) 7.2.2 (3, N=6) 7.5.4 (15, N=3) 6.22.5 (, N=) 5.25.2 (1, N=2) zoea II 6.24.1 (5, N=1) 6.15.13 (35, N=7) 5.2.33 (25, N=5) 4.5.1 (5, N=1) 5.11.3 (1, N=2) zoea III 5.73.11 (55, N=11) 7.25.5 (35, N=7) 5.3.21 (2, N=4) 3.15.1 (5, N=1) 5.2.23 (35, N=7) zoea IV 6.17.4 (5, N=1) 7.1.2 (4, N=8) 7.11.1 (25, N=5) 5.13.15 (1, N=2) 8.14.31 (35, N=7) 1 Mortality was 1% at, 5 and 1 salinity in each stage within 12 hours after exposure. 2 MeanSD (days). 3 Mortality in percentage (%) with number of dead individuals (N). zoea V 4.61.2 (6, N=12) 8.2.22 (4, N=8) 6.19.3 (25, N=5) 4.25.5 (15, N=3) 6.15.7 (2, N=4) zoea VI (1, N=2) 8.1.15 (55, N=11) 7.15.5 (25, N=5) 5.2.43 (15, N=3) 6.15.14 (45, N=9)
Effect of Salinity on the Larval Development of Charybdis natator zoeal stages of Charybdis natator during the experimental period. Zoea I larvae showed a decrease in the development duration from 7.25 to 5.25 days with increasing salinities from 15 to 35, whereas at the lower salinities,, 5 and 1, they did not moult to the subsequent stages within 12 hours of exposure. Zoea VI larvae died upon exposure to 15 salinity (Table 1). Development duration for zoeae II to VI stage was shortest at 3 (4.5, 3.15, 5.13, 4.25 and 5.2 days, respectively) (Table 1). At 2 to 35 salinity, a total of 45% of the zoeae VI died before moulting into the megalopa and the remainder (55%) died within 6 hours after moulting into the megalopa. Few zoeae successfully moulted to megalopa. The highest mortality before moulting was observed, in decreasing order, at, 5, 1, 15, and 2. The lowest mortality of zoea I (%), zoeae II and III (5%), zoea IV (1%), and zoeae V and VI (15%) were recorded at 3 (Table 1). Zoeal mortality gradually decreased with increasing salinity up to 3. The highest survival rate was found at 3 (zoea I, 1%; zoeae II and III, 95%; zoea IV, 9%; zoeae V and VI, 85%) followed by 35 and 25 in all zoeal stages throughout the experimental period (Fig. 1). The survival rate of zoea larvae gradually increased with salinity (P.5; Fig. 1) up to 3. ANOVA detected a significant difference (P.1) in survival rate at the different salinity levels (Fig. 1). Dunnett s Multiple Comparison test indicated a significant difference (P.5) between 3 and 15, 3 and 2, and 25 and 15 salinity (Table 2). Development duration of each zoeal stage at each salinity level is also recorded in Table 1. Hatched larvae required an average 35 days to moult into the megalopa stage. Total duration required for zoea I to moult into megalopa 125 1 75 5 25 Zoea I Zoea II Survival (%) 125 1 75 5 25 Zoea III Zoea IV 125 1 75 5 25 Zoea V Zoea VI 15 2 25 3 35 15 2 25 3 35 Salinity () Survival (%) of zoeae I to VI of Charybdis natator (Herbst) at different salinity level. Vertical bars indicate plus standard deviation.
M. S. Islam, S. Shokita, and Y. Fujita stage at 2, 25, 3 and 35 was 44, 38, 28 and 36 days, respectively (Fig. 2). Dunnett s Multiple Comparison test indicated significant differences (P.5) in total duration of zoeal development between salinities at 35 and 2, 3 and 2, 25 and 15, and 2 and 15 (Fig. 2). Similarly, development duration differed significantly between 3 and 15, 3 and 2, 25 and 15, 35 and 15, and 2 and 15 salinity (Table 3). We found that the salinity ranges suitable for zoeal development were 3 to 35 for zoeae I, II and V, 25 to 3 for zoeae III and IV, and 3 for zoea VI (Fig. 3). Table 4 compares these values with other brachyuran crab species. Comparison of survival rate between zoeae I to VI of Charybdis natator (Herbst) at different salinity levels (Dunnett s Multiple Comparison test) Salinity () 3 vs 15 3 vs 2 3 vs 35 3 vs 25 25 vs 15 25 vs 2 25 vs 35 35 vs 15 35 vs 2 2 vs 15 Total duration (days) 8 7 6 5 4 3 2 1 SE 5.135 5.135 5.135 5.135 Q 4.284 3.251 1.821 1.549 2.88 1.72.272 2.548 1.43 1.185 15 2 25 3 35 Salinity () Conclusion Note: if QQ (.5, 5) = 2.87 then significant (), else not significant (), Order: 3, 25, 35, 2, and 15. Duration required for the zoeal development of Charybdis natator (Herbst) at different salinity levels. Vertical bars indicate plus standard deviation. Comparison of duration between zoeae I to VI of Charybdis natator (Herbst) at different salinity levels (Dunnett s Multiple Comparison test) Zoeal stages Salinity () 3 vs 15 3 vs 2 3 vs 35 3 vs 25 25 vs 15 25 vs 2 25 vs 35 35 vs 15 35 vs 2 2 vs 15 Zoea VI Zoea V Zoea IV Zoea III Zoea II Zoea I SE 5.165 5.165 5.165 5.165 Suitable salinity 5 1 Q 1.584 6.261 3.553 2.877 7.841 3.384.677 7.196 2.77 4.614 15 2 25 3 35 Salinity () Salinity suitable for the zoeal development of Charybdis natator (Herbst). Vertical bars indicate standard deviation. Discussion Portunid crabs commonly breed from April through September and thus, during this time ovigerous females are readily available 19). We found that salinity significantly influenced zoeal development of Charybdis natator and the shortest duration required for complete zoeal development to megalopa stage was 28 days at 2 to 35. In contrast, the edible portunid crab T. crenata requires 26 days at 35 19). In the present study, the highest mortality (1%) was recorded at low salinities (, 5 and 1) within 12 hours after exposure. Larval mortality at low salinity is potentially due to an imbalance in osmoregulatory mechanisms 19). Many of the zoea larvae died during moulting. Only 55% (average) zoea VI larvae moulted into the megalopa stage and survived for only 6 hours Conclusion Note: if QQ (.5, 5) = 4.166 then significant (), else not significant (), Order: 3, 25, 35, 2, and 15.
Effect of Salinity on the Larval Development of Charybdis natator Suitable salinity and temperature for the larval development of brachyuran crabs Species Charybdis natator Charybdis cruciata Charybdis bimaculata Portunus spinicarpus Scylla serrata Scylla serrata Scylla serrata Thalamita crenata Necora puber Callinectes sapidus Chasmagnathus convexus Armases miersii Armases ricordi Armases roberti Helice formoseis Helice leachi Salinity () 25-35 32-34 32-34 3-35 25-3 25-3 25-3 25-35 3-35 25-35 21-28 15-45 2-45 15-45 2-3 15-2 Temp. () 22-25 28-31 21-24 2-25 25-28 26-3 25-28 26-28 2-25 25-3 17-2 22-25 23-26 23-26 2-22 21-24 References This paper Motoh and Villaluz 16) Hwang and Kim 1) Bookhout and Costlow 39) Saha et al. 37) Chen and Cheng 38) Raphel 36) Kannupandi et al. 19) Mene et al. 22) Costlow 27) Islam et al. 32) Schuh and Diesel 25) Diesel and Schuh 31) Diesel and Schuh 31) Mia and Shokita 34) Mia and Shokita 34) thereafter. This may be due to a change in stage, poor feeding practice, environmental conditio, or substratum. Larval development of T. crenata goes to completion only at 25 to 35 19). Likewise, the salinity and temperature combinatio for rearing larvae of S. serrata and C. bimaculata (Miers) are 25 to 3 at 25 to 28 and 32 to 34 at 21 to 24, respectively (Table 4) 1,36,37). The highest mortality of portunid crab larvae (N. puber) occurs at 3 at 25 22). Conversely, we found that zoeal development for C. natator was completed only in 25 to 35 at 22 to 25 (Table 4). Optimal levels of salinity and temperature ranged from 25 to 3 at 26 to 3 for the survival of S. serrata larvae and 32 to 34 at 28 to 31 for C. cruciata (Herbst) (Table 4) 16,38). Highest survival of Portunus spinicarpus Stimpson larvae are recorded at 3 to 35 39). The highest observed survival for C. sapidus is in 25 to 35 at 25 to 3 27). The larvae of S. serrata show coiderable mortality at salinities below 17.5, although juveniles survive at 2 for at least 12 days and tolerate salinities as high as 6 3). However, the juveniles of S. serrata survived for only 4 days when they were traferred from 35 to salinities lower than 32 29). The range of optimal salinity for the development of larvae to megalopae for A. ricordi is 2 to 45 and 15 to 45 for A. roberti. Maximum survival of zoeae (8%) and megalopae (9%) of H. leachi is recorded in 2 and 15 at 24, and the highest survival of zoeae (1%) and megalopae (1%) of H. formoseis is recorded in 3 and 2 at 22 (Table 4) 34). Larval survival of A. miersii was frequently higher at 15 to 25 than at 35 27). The highest survival of zoea II of S. angustipes is recorded at 3 to 32 salinity, indicating a decreasing tolerance to low salinity ( to 1) and a shift in optimum salinity toward seawater (35) in successive zoeal stages 26). Significant survival for zoeae (95%) and megalopae (75%) of C. convexus is recorded at 21 to 28 32). Larval development for C. irroratus is completed at 3 to 35 at 15 33). Suitable salinities are those in which the larval survival rate is very high (above 8%), shorter duration is required for moulting into the subsequent stage, larvae are actively feeding and swimming, growth rate is very high and the physical condition of larvae is very good. In the present study, suitable salinity required for the development of each larval stage varied. The most suitable salinity for rearing zoeae I to VI of C. natator was between 25 and 35, and the highest survival rate (about 95% in all cases) was at 3. Thus, salinity between 25 and 35 may be coidered suitable for rearing of C. natator zoeae. The larvae of Scylla sp. go through a freeswimming period in the sea and then at the megalopa stage, they migrate into the brackish water where reside until death 4). Adult of S.
M. S. Islam, S. Shokita, and Y. Fujita serrata inhabit pools and puddles in the littoral zone (intertidal zone) and then migrate into estuaries after mating 36), where their zoeae are released into the seawater. After moulting, juveniles migrate into the littoral zone where they are likely to encounter reduced salinity 5,41). The present results suggest that free swimming C. natator zoeae may reside in offshore water and then move to brackish water at the megalopa stage, where they spend the rest of their life. To improve larval rearing, further studies are necessary, which investigate the physiological influence of different salinity levels on metamorphosis and osmoregulation of this species. Acknowledgments We are greatly indebted to Dr. N. Shikatani of the University of the Ryukyus, Dr. M. Abid of Pakistan, and Dr. L. P. Vidhana of Sri Lanka for their critical review and valuable suggestio towards the improvement of this manuscript. We also express our sincere thanks to Mr. T. Nagai and Mr. T. Higa for their kind cooperation in sampling and laboratory maintenance. Thanks are also extended to two anonymous reviewers, and Dr. Natalie Karouna-Renier of Bioscience editor for reviewing and editing this manuscript carefully. References 1 ) Hwang, S. G. and C. H. Kim (1995): Complete larval development of the swimming crab, Charybdis bimaculata (Miers, 1886) (Crustacea, Brachyura, Portunidae), reared in laboratory. Korean J. Zool.,, 465-482. 2 ) Stepheon, W., J. J. Hudson, and B. Cambell (1957): The Australian portunids (Crustacea: Portunidae), II. The genus Charybdis. Australian J. Mar. Freshwater Res., (4), 491-57. 3 ) Menon, M. K. (1952): A note on the bionomics and fishery of the swimming crab Neptunus sanguinolentus (Herbst) on the Malabar Coast. J. Zool. Soc. India,, 177-184. 4 ) Dai, A. and S. Yang (1991): Crabs of the China Seas. China Ocean Press, Beijing, China, 682 pp. 5 ) Dai, A., S. Yang, Y. Song, and G. Chen (1986): Crabs of the China Seas. China Ocean Press, Beijing, China, 568 pp. 6 ) Sakai, T. (1976): Crabs of Japan and the Adjacent Seas. Kodaha Ltd., Tokyo, Japan, 773 pp. 7 ) Spiridonov, V. (1999): Results of the Rumphius Biohistory Expedition to Ambon. Part 8. Swimming crabs of Ambon (Crustacea: Decapoda: Portunidae). Zoologische Mededelingen,, 63-97. 8 ) Wee, D. P. C. and P. K. L. Ng (1995): Swimming crabs of the genera Charybdis De Haan, 1833, and Thalamita Latreille, 1829 (Crustacea: Decapoda: Brachyura: Portunidae) from Peniular Malaysia and Singapore. The Raffles Bulletin of Zoology,, 1-128. 9 ) Yatsuzuka, K. (1952): The metamorphosis and growth of the larva of Charybdis japonica A. Mine Edward. Bull. Japanese Soc. Sci. Fish., (11), 17-22. 1) Hashimi, S. S. (1969): The brachyuran larvae of West Pakistan hatched in the laboratory. Part II. Portunidae: Charybdis (Decapoda: Crustacea). Pakistan J. Sci. Indus. Res.,, 272-278. 11) Fielder, D. R., J. C. Greenwood, and G. Campbell (1984): The megalopa of Charybdis feriata (Linnaeus) with additio to the zoeal larvae descriptio (Decapoda, Portunidae). Crustaceana,, 16-165. 12) Greenwood, J. C. and D. R. Fielder (198): The zoeal stages and megalopa of Charybdis callianassa (Herbst) (Decapoda, Portunidae) reared in laboratory. Proc. Royal Soc. Queeland,, 61-76. 13) Kurata, H. (1975): Larvae of decapod brachyura of Arasaki, Sagami Bay, V. The swimming crabs of subfamily Portuninae. Bull. Naei Reg. Fish. Res. Lab.,, 39-65. 14) Kurata, H. and S. Nishina (1975): The zoeal stages of the swimming crab, Charybdis acuta. Bull. Tokai Reg. Fish. Res. Lab.,, 129-136. 15) Kurata, H. and H. Omi (1969): The zoeal stages of the swimming crabs, Charybdis japonica and Portunus hastatoides reared in the laboratory. Bull. Naei Reg. Fish. Res. Lab.,, 21-27. 16) Motoh, H. and A. Villaluz (1976): Larvae of decapod crustacea of the Philippines, I. The zoeal stages of a swimming crab, Charybdis cruciata (Herbst) reared in the laboratory. Bull. Japanese Soc. Sci. Fish., (5), 523-531. 17) Terada, M. (1979): A classification of zoea larvae in the subfamily Portuninae of the family Portunidae. Zool. Mag. Tokyo,, 254-268. 18) Yatsuzuka, K., K. Sakai, and N. D. Roman (1984): The larvae and juvenile crabs of Japanese Portunidae (Crustacea, Brachyura), III. Charybdis japonica A. Mine Edward. Rep. Usa Mar. Biol. It., Kochi Univ.,, 23-4. 19) Kannupandi, T., T. Krishnan, and A. Shanmugam (1997): Effect of salinity on the larvae of an edible estuarine crab Thalamita crenata (Crustacea: Decapoda: Portunidae). Indian J. Mar. Sci.,, 315-318. 2) Sumpton, W. D. (199): Biology of the rock crab, Charybdis natator Herbst (Brachyura: Portunidae). Bull. Mar. Sci., (2), 425-431. 21) Anger, K. (1991): Effects of temperature and salinity on the larval development of the Chines mitten crab Eriocheir sineis (Decapoda: Grapsidae). Mar. Ecol. Prog. Ser.,, 13-11. 22) Mene, L., M. T. A. Ossorio, E. G. Gurriaran, and L. Valdes (1991): Effects of temperature and salinity on
Effect of Salinity on the Larval Development of Charybdis natator larval development of Necora puber (Brachyura: Portunidae). Marine Biology,, 73-81. 23) Minagawa, M. (1992): Effects of salinity on survival, feeding and development of larvae of the red frog crab Ranina ranina. Nippon Suisan Gakkaishi, (1), 1855-186. 24) Anger, K. (1996): Salinity tolerance of the larval and first juveniles of a semi terrestrial grapsid crab, Armases miersii (Rathbun). J. Exp. Mar. Biol. Ecol.,, 25-223. 25) Schuh, M. and R. Diesel (1995a): Effects of salinity, temperature, and starvation on larval development of Armases (=Sesarma) miersii (Rathbun), a semiterrestraial crab with abbreviated development (Decapoda: Grapsidae). Journal of Crustacean Biology, (2), 25-213. 26) Anger, K., J. Harms, M. Montu, and C. D. Bakker (199): Effects of salinity on the larval development of a semi terrestrial tropical crab, Sesarma angustipes (Decapoda: Grapsidae). Mar. Ecol. Prog. Ser.,, 89-94. 27) Costlow, J. D. (1967): The effect of salinity and temperature on survival and metamorphosis of megalops of the blue crab Callinectes sapidus. Helgolander Wissechaftlische Meeresuntersuchungen,, 84-97. 28) Guerin, J. L. and W. B. Stickle (199): Effects of salinity on the tolerance and bioenergetics of the blue crab Callinectes sapidus. Bull. Mar. Sci., (1), 245-246. 29) Davenport, J. and T. M. Wong (1987): Respoe of adult mud crabs (Scylla serrata Forskal) to salinity and low oxygen teion. Comp. Biochem. Physiol.,, 43-47. 3) Hill, B. J. (1974): Salinity and temperature tolerance of zoeae of the portunid crab Scylla serrata. Marine Biology,, 21-24. 31) Diesel, R. and M. Schuh (1998): Effects of salinity and starvation on larval development of the crabs Armases ricordi and A. roberti (Decapoda: Grapsidae) from Jamaica, with notes on the biology and ecology of adults. Journal of Crustacean Biology, (3), 423-436. 32) Islam, M. S., S. Shokita, and H. Kawaguchi (2): Effects of salinity on the larval development of a sesarmid crab, Chasmagnathus convexus (De Haan). Bull. Fac. Sci., Univ. Ryukyus,, 35-43. 33) Joh, D. M. (1981): Physiological studies on Cancer irroratus larvae, 1. Effects of temperature and salinity on survival, developments rate and size. Mar. Ecol. Prog. Ser.,, 323-329. 34) Mia, M. Y. and S. Shokita (1997): Optimal salinity required for the larval development of two grapsid crabs, Helice leachi Hess and H. formoseis Rathbun. Crustacean Research,, 7-74. 35) Schuh, M. and R. Diesel (1995b): Effects of salinity, and starvation on larval development of Sesarma curacaoee (De Man), a mangrove crabs with abbreviated development (Decapoda: Grapsidae). Journal of Crustacean Biology, (4), 645-654. 36) Raphel, Y. I. (1972): Preliminary report on the brackish water pond culture of Scylla serrata (Forskal) in Ceylon, in Coastal aquaculture in the Indo-Pacific Region (ed. by T. V. R. Pillay). West fleet fishing news books, pp. 35-395. 37) Saha, M. R., P. K. Roy, and S. U. Ahmed (1997): Impact of stocking deity on growth and survival rate of mud crab (Scylla serrata Forskal). Bangladesh J. Fish. Res., (1), 47-52. 38) Chen, H. C. and K. H. Cheng (198): Studying on the larval rearing of mud crab Scylla serrata. China Fisheries Monthly,, 3-8. 39) Bookhout, C. G. and J. D. Costlow (1974): Larval development of Portunus spinicarpus reared in the laboratory. Bull. Mar. Sci.,, 2-51. 4) Ohshiro, N. (1991): Mangrove crab (Scylla sp.), in Aquaculture in Tropical Areas (ed. by S. Shokita, K. Kakazu, A. Tomori and T. Toma). Midori Shobo Co. Ltd., Tokyo, pp. 218-229. 41) Hill, B. J., M. J. Williams, and P. Dutton (1982): Distribution of juvenile, subadult and adult Scylla serrata (Crustacea: Portunidae) on tidal flats in Australia. Marine Biology,, 117-12.
M. S. Islam, S. Shokita, and Y. Fujita Md. Sirajul ISLAM 35 5 8 235 24425383283536 1 2535 2353