Release of 'Norwest 553' Hard Red Winter Wheat

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Release document for Norwest 553 (approved 8-20-07) Release of 'Norwest 553' Hard Red Winter Wheat Norwest 553 is a hard red winter wheat (Triticum aestivum L.) developed by Oregon State University and Nickerson International Research SNC, a division of Limagrain Verneuil Holding SA, in cooperation with USDA-Agricultural Research Service. Norwest 553 is being released for its high yield potential, disease resistance, and milling and baking quality which is considered acceptable for the hard red winter wheat market class. Norwest 553 was derived from the cross 958343/lsengrain made in 1996 by the Verneuil Company. The experimental line 958343, also known as Lander3, was bred by Verneuil. Isengrain was bred by Florimond Desprez and registered for production in France in 1996. Norwest 553 was derived as a doubled haploid from an F1 plant in the 1997-1998 growing season and given the selection designation 008553. From 1998 to 2001, 008553 was evaluated in France in Verneuil breeding trials. In 2001, Verneuil was acquired by Limagrain Agro-lndustrie S.A. In fall, 2001, seed of 008553 was provided to Oregon State University under a germplasm exchange agreement with Limagrain Agro-Industries. The selection was subsequently tested in Oregon under the experimental designation ORN008553. A sister line, 008556, was registered for production in Italy in 2004 under the name Copernico. Norwest 553 is a semidwarf variety best adapted to moderate to high rainfall areas of north east Oregon and south east Washington. Crown freezing tests conducted by USDA-ARS suggest that Norwest 553 has cold tolerance similar to the soft white winter variety Tubbs; greater than Stephens, but less than the hard red winter variety Paladin. Field evaluations conducted by the WSU Cereal Variety Testing program suggest Norwest 553 has winterhardiness similar to Stephens, less than both Paladin and Tubbs. Winter damage on Norwest 553, as indicated by slow recovery and reduced growth in early spring, increased when association with other stress conditions, such as drought or late fall plantings. In USDA-ARS stripe rust (Puccinia striiformis Westend.) evaluations, Norwest 553 has a resistant reaction type with low infection intensities; similar to Stephens, less than Paladin or 8oundary. Norwest 553 has an intermediate response to strawbreaker footrot (Psuedocercosporella herpotrichoides (Fron.) Deighton) and does not carry the Pch-1 gene for resistance from VPM-1. In inoculated trials, Norwest 553 has shown moderate levels of resistance to cephalosporium stripe (Cephalosporium gramineum Nis. & Ika.). Disease ratings were similar to those for Madsen, lower than for Stephens or Tubbs. Norwest 553 is moderately susceptible to Septoria leaf blotch (Septoria tritici Roberge in Desmaz.), with disease ratings similar to Madsen or Tubbs. Norwest 553 was evaluated in the 2005 and 2006 Oregon hard wheat variety trials and in the 2006 Washington hard wheat variety trials. Over 20 site by years of combined

Oregon and Washington testing, Norwest 553 averaged 82 bu/a, as compared with Paladin and Bauermeister at 75.5 and 72.8 bu/a, respectively. In 2006, grain test weight of Norwest 553 averaged 61.7 Ib/bu, higher than that for Boundary at 61.0 Ib/bu, but 0.9 Ib/bu lower than for Paladin. Grain protein concentration of Norwest 553 averaged 11.7%, similar to Paladin and 0.5 percentage points higher than for either Bauermeister or Boundary. Heading date for Norwest 553 is similar to Boundary, approximately 1 day later than Paladin and 3 days earlier than Bauermeister. Plant height of Norwest 553 averages 72 cm, 8 cm less than Paladin and 15 cm less than Bauermeister. End-use quality of Norwest 553 was evaluated through the USDA-ARS Western Wheat Quality Lab in 2005 and 2006. Results from the milling and baking evaluations suggest that Norwest 553 has acceptable quality for the hard red winter market class. Dough mixing strength is its most positive attribute. When limited to paired observations with over 10.4% flour protein, Norwest 553 was similar to Paladin and Bauermeister for flour yield, flour ash, and bake absorption. Milling score for Norwest 553 was slightly higher than for Paladin, similar to Bauermeister. Norwest 553 had a significantly longer mixing time, averaging over 5 min as compared with 4 min for Paladin and 3 min for Bauermeister. Bread loaf volumes for Norwest 553 averaged slightly less than expected, 40 cc less than Paladin, but with good internal crumb grain scores. Norwest 553 was evaluated by the PNW Wheat Quality Council in 2007. Norwest 553 was considered acceptable for the hard red winter class. In milling comparisons with Bauermeister, Norwest 553 had lower flour yield, but with significantly lower flour ash. It was considered generally superior to Bauermeister for dough mixing and handling properties, loaf volume, internal loaf structure, and overall baking quality. Dough water absorption was considered slightly less than optimal. In fall, 2005, 750 heads of Norwest 553 were threshed, screened for seed color and seed size, and provided to Washington Foundation Seed for production of Breeder seed. These were planted as individual headrows and off-type rows were removed prior to bulk harvest of Breeder seed. Breeder and Foundation seed will be maintained by Washington State Crop Improvement Association (WSCIA). Norwest 553 is being submitted for Plant Variety Protection with the Title 5 option. Certification classes recognized for Norwest 553 include Foundation, Registered and Certified. Certified seed will be produced and sold under non-exclusive license with Oregon State University. Seed of Norwest 553 has been deposited in the USDA National Small Grains Collection, Aberdeen, Idaho. It is requested that the source of this material be acknowledged in future use by wheat breeding and genetics programs. Acknowledgements: Appreciation is extended to the Oregon Wheat Commission for financial support in the development of Norwest 553. OSU Contributors: C.J. Peterson, M. Verhoeven, M. Larson, B. Hoefer, J. von Zitzewitz, M. Flowers, A. Ross, and J. Ohm, Dep. of Crop and Soil Science, Oregon State University, Corvallis, OR, 97331; C. Morris and D. Engle, USDA-ARS Western

Wheat Quality Laboratory, Washington State University, Pullman, WA, 99164; R. Smiley, Columbia Basin Agricultural Experiment Station, Oregon State University, Pendleton, OR, 97801; C. Mundt, Dep. of Botany and Plant Pathology, Oregon State University, Corvallis, OR, 97331; X. Chen, D. Skinner, and K. Campbell, USDA-ARS, Johnson Hall, Washington State University, Pullman, WA, 99164; J. Robinson, Foundation Seed Service, Washington State Crop Improvement, Washington State University, Pullman, WA, 99164.

Grain yield, test weight, and protein summary for HRW selection NORWEST 553. Grain Yield 2006 HWELT 2005 HWELT 2006 WSU Trials HWEYT 07 WSU07 McGregor 07 2006 7-site 6 sites 7 - site 7-site 9 sites 9 - site Entrv No. Selection bu/a rank bu/a rank bu/a rank bu/a bu/a bu/a 1 NORWEST553 76.9 4 83.7 6 85.7 7 85.3 74.2 103.2 2 Paladin 72.3 22 70.0 20 83.3 12 79.7 71.2 94.9 3 Eddy 85.6 8 80.2 76.2 93.8 4 Boundary 70.8 26 95.1 3 78.6 81.4 5 Declo 71.4 25 75.4 13 81.7 65.5 6 Bauermeister 66.3 30 70.3 19 81.4 14 81.7 81.7 7 Tubbs 06 77.7 3 88 100.9 Grain yield, test weight, and protein summary for HRW selection NORWEST 553. TestWt. 2006 TestWeiaht 2007 Grain Protein '06 Grain Protein '07 2006 2006 2007 2006 2006 2006 2006 2007 2007WSU HWELT WSU HWELT WSU McGr. HWELT WSU HWELT Trials 2006 6-sites Trials 7-sites Trials Trials 6-sites Trials 7-sites 9-site Entrv No. Selection Ib/bu Ib/bu Ib/bu Ib/bu Ib/bu % % % % 1 NORWEST553 62.5 61 63.0 61.3 60.7 11.2 12.2 10.5 12.9 2 Paladin 63.0 62.3 63.3 61.4 60.8 11.0 12.2 11.0 13.1 3 Eddy 62.9 63.0 61.7 59.8 12.0 10.3 12.7 4 Boundary 61.0 61 61.2 60.3 10.6 11.6 10.2 12.4 5 Declo 61.8 63.0 61.1 10.4 10.6 13.4 6 Bauermeister 59.1 59.3 61.4 60 10.7 11.7 10.1 12.1 7 Tubbs 06 59.2 60.0 57.4 9.8 10.0

Agronomic summary for HRW selection NORWEST 553 grown in the HWELT. Heading date Plant height (cm) (from 1/1) 2006 2005 2006 2007 2006 2006 OWEYT OWEYT WSU OWEYT Mean WSU Entry No. Selection 7 sites 6 sites 8 sites 6 sites 27 site 8 sites 1 NORWEST553 71.1 75.8 70.8 75.7 73 147.9 2 Paladin 79.7 82 78.2 83.1 80.5 146.6 3 Eddy 78.9 86.6 145.5 4 Boundary 81 80.9 86.4 148.4 5 Declo 75.5 77.7 80.5 6 Bauermeister 87.3 84.4 89.8 89.7 87.9 151 7 Tubbs 06 86.4 83.8 93.5

Evidence of Uniformity and Stability Norwest 553 has been observed to be uniform and stable based on evaluations in multilocation replicated yield trials. From 2005 through 2007, Norwest 553 was evaluated in a total of 45 replicated yield trials in Oregon and Washington. Norwest 553 hard red winter wheat may contain up to 20 white seed per pound in Breeders, Foundation, Registered, or Certified classes of seed multiplication. Norwest 553 also may contain up to 1 in 10,000 combined of the naturally occurring variants: plants that are 2" to 6" taller; plants with awnless spike; and plants with bronze (red or tan) chaff spikes. Variations in plant height will be more distinct when grown in environments that are conducive to high grain yield, including irrigated or high rainfall conditions. These variants described are distinct within the variety and are stable and predictable with a degree of reliability comparable to other varieties of the same kind, and within recognized tolerances, when the variety is reproduced or reconstructed and was originally part of the variety when released. To further determine variants in kernel color, a phenol staining reaction was determined. It was observed that 39% of kernels stained are light brown, 55% are brown, and 6% are dark brown.