Hatchery and Supplementation Program DRAFT 2014 Report. Lewis River Hydroelectric Projects FERC Project Nos. 935, 2071, 2111, 2213

Similar documents
Hatchery and Supplementation Program 2013 Annual Report

Hatchery and Supplementation Program FINAL 2015 Report. Lewis River Hydroelectric Projects FERC Project Nos. 935, 2071, 2111, 2213

Lewis River Hydroelectric Projects Settlement Agreement Aquatic Coordination Committee (ACC) Meeting Agenda

2013 WHITE SALMON CHINOOK SALMON VSP MONITORING. Jeremy Wilson Washington Department of Fish and Wildlife

Lewis River Hydroelectric Projects Settlement Agreement Aquatic Coordination Committee (ACC) Meeting Agenda

Hatchery Scientific Review Group Review and Recommendations

Grande Ronde Basin Spring Chinook Salmon Captive Broodstock Program: F 1 Generation

PLEASE BRING YOUR LUNCH

Lewis River Hydroelectric Projects Settlement Agreement Aquatic Coordination Committee (ACC) Meeting Agenda

LOWER COWLITZ ADULT TRIBUTARY ABUNDANCE METHODS

ASSESSMENT OF THE STATUS OF NESTUCCA RIVER WINTER STEELHEAD

Hatchery Scientific Review Group Review and Recommendations

Reproductive success of hatchery chinook salmon in the Deschutes River, Washington

2018 Annual Operating Plan

Summary of HSRG Findings for Chum Populations in the Lower Columbia River and Gorge

Steelhead Kelt Reconditioning and Reproductive Success Studies in the Columbia River Basin

LIFE HISTORY DIVERSITY AND RESILIENCE

APPENDIX D: LEWIS RIVER HATCHERY REVIEW

Hatchery Scientific Review Group Review and Recommendations

Hood Canal Steelhead Project A conservation hatchery experiment. Joy Lee Waltermire

Yakima River Basin Coho Reintroduction Feasibility Study

Lewis River Hydroelectric Projects Settlement Agreement Aquatic Coordination Committee (ACC) Meeting Agenda

Hatchery Scientific Review Group Review and Recommendations

THE CONFEDERATED TRIBES OF THE WARM SPRINGS RESERVATION OF OREGON

LOWER SNAKE RIVER COMPENSATION PLAN: Oregon Spring Chinook Salmon Evaluation Studies 2006 Annual Progress Report

Yale Reservoir Kokanee (Oncorhynchus nerka) Escapement Report 2016

LOWER SNAKE RIVER COMPENSATION PLAN: Oregon Spring Chinook Salmon Evaluation Studies 2007 Annual Progress Report

EVALUATION OF FALL CHINOOK AND CHUM SALMON SPAWNING BELOW BONNEVILLE DAM

index area in Pine Creek mainstem to establish redd-life

Addressing Critical Uncertainties in the Reintroduction of Chum Salmon to Oregon Tributaries of the Columbia River. Kris Homel

ANNUAL PROGRESS REPORT FISH RESEARCH PROJECT OREGON. PROJECT TITLE: Spring Chinook Salmon in the Willamette and Sandy Rivers

Lewis River Upstream Transport Plan Interim Final. Prepared by Frank Shrier Principal Fish Biologist PacifiCorp Energy.

Downstream Migrant Trapping in Russian River Mainstem, Tributaries, and Estuary

Job Performance Report, Project F-73-R-9 Subproject II: SALMON AND STEELHEAD INVESTIGATIONS Study I: Salmon Spawning Ground Surveys

NEVADA DEPARTMENT OF WILDLIFE STATEWIDE FISHERIES MANAGEMENT

Work Completed for Compliance With the Biological Opinion for Hatchery Programs in the Willamette Basin, USACE funding: 2003

Salmon and Steelhead Spawning Survey Methods Stratification of Estimates

Factors that affect steelhead and salmon catch and release survival in freshwater sport fisheries throughout Washington state

OXBOW FISH HATCHERY AND HELLS CANYON FISH TRAP

Downstream Migrant Trapping in Russian River Mainstem, Tributaries, and Estuary

Steve Hemstrom Sr. Fisheries Biologist Chelan PUD Natural Resources Desk: Cell:

***Please Note*** April 3, Dear advisory committee members:

Feather River Fish Hatchery spring run Chinook salmon Program: moving toward conservation. Ryon Kurth, Jason Kindopp, Anna Kastner, and A.J.

BOGUS CREEK SALMON STUDIES 2002

CUSHMAN RESERVOIRS. Skokomish Watershed Monitoring Conference - Public Meeting Florian Leischner 9/17/2015

Strategies for mitigating ecological effects of hatchery programs

Appendix B. Data on Aquatic Focal Species: Adult returns to TMFD, Disposition, Escapement, Artificial Production, and Harvest

OREGON AND WASHINGTON DEPARTMENTS OF FISH AND WILDLIFE JOINT STAFF REPORT - FALL FACT SHEET NO.

Fish Habitat Restoration and Monitoring in Southeast Washington. Andy Hill Eco Logical Research, Inc.

Juvenile chum migration patterns in the lower Columbia River and estuary

c h a p t e r 6 n n n Related to the VAMP

Wild Steelhead Coalition Richard Burge Conservation VP September 11, 2006

Monitoring of Downstream Fish Passage at Cougar Dam in the South Fork McKenzie River, Oregon February 8, By Greg A.

Attachment 1. Agenda Item Summary BACKGROUND

NATIVE FISH CONSERVATION PLAN FOR THE SPRING CHINOOK SALMON ROGUE SPECIES MANAGEMENT UNIT

Yakima/Klickitat Fisheries Project

Meeting Summary Fisheries Management Technical Feedback Group Meeting Friday, November 2, 2012

Selective Fisheries. What is Selectivity and how is it used in Columbia River Fisheries?

Don Pedro Project Relicensing

Upper Columbia Redband Trout: Conservation for the Future

OREGON DEPARTMENT OF FISH AND WILDLIFE ROGUE FISH DISTRICT REPORT

Hatcheries: Role in Restoration and Enhancement of Salmon Populations

JOINT STAFF REPORT WINTER FACT SHEET NO. 9 Columbia River Compact March 18, 2004

Brian Missildine Natural Resource Scientist Hatchery Evaluation and Assessment Team Lead Washington-British Columbia Annual General Meeting Kelowna,

OREGON AND WASHINGTON DEPARTMENTS OF FISH AND WILDLIFE JOINT STAFF REPORT: SUMMER FACT SHEET NO. 1 Columbia River Compact June 13, 2012

Perspectives of a State Director Selective fisheries as a tool in fisheries management and salmon recovery

FINAL HATCHERY AND GENETIC MANAGEMENT PLAN (HGMP)

Justification for Rainbow Trout stocking reduction in Lake Taneycomo. Shane Bush Fisheries Management Biologist Missouri Department of Conservation

2015 Adult Returns and 2016 Expectations Columbia River Preliminary Draft December 14, 2015

CHAPTER 2 - THE COQUILLE FISHERY

TAY DISTRICT SALMON FISHERIES BOARD POLICY ON SALMON STOCKING

PLEASE BRING YOUR LUNCH

Rivers Inlet Salmon Initiative

Sandy River Fisheries Management Update. Todd Alsbury District Fish Biologist (Cascade Unit)

Wenatchee Spring Chinook Relative Reproductive Success Study. Andrew Murdoch (WDFW) Michael Ford (NOAA) Michael Hughes (WDFW)

Kirt Hughes Washington Department of Fish and Wildlife Region 6 - Fish Program Manager

Recreational Sturgeon Commercial Shad MANAGEMENT GUIDELINES

Importance of water temperature in the management of American river Chinook Salmon and steelhead:

LEWIS RIVER FISH PLANNING DOCUMENT

Coho. Oregon Native Fish Status Report 13

18 March 2016 Oregon Department of Fish and Wildlife

The Fishery. Newfoundland Region Stock Status Report D2-05

LOWER MOKELUMNE RIVER UPSTREAM FISH MIGRATION MONITORING Conducted at Woodbridge Irrigation District Dam August 2014 through July 2015.

Study Update Fish Distribution and Species Composition

U. S. Fish and Wildlife Service Connecticut River Coordinator s Office. Ken Sprankle Connecticut River Coordinator

2015 Winnebago System Walleye Report

FINAL Meeting Notes Lewis River License Implementation Aquatic Coordination Committee (ACC) Meeting September 14, 2006 Ariel, WA

OREGON AND WASHINGTON DEPARTMENTS OF FISH AND WILDLIFE JOINT STAFF REPORT SUMMER FACT SHEET NO. 1 June 10, 2010

2011 upper Lewis River Bull Trout Investigations. Jim Byrne

MID-COLUMBIA FISH DISTRICT ANNUAL REPORT Fish Liberations

Conditions affecting the 2011 and 2012 Fall Chinook Adult Returns to Spring Creek National Fish Hatchery.

Salmon age and size at maturity: Patterns and processes

Eulachon: State of the Science and Science to Policy Forum

Minnesota Department of Natural Resources Fisheries Division, Lake Superior Area

A genetic analysis of the Summer Steelhead stock composition in the 2011 and 2012 Columbia River sport and treaty fisheries

FALL FACT SHEET NO. 2 Columbia River Compact August 13, 2004 MANAGEMENT GUIDELINES

2012 JOINT STAFF REPORT: STOCK STATUS AND FISHERIES FOR FALL CHINOOK SALMON, COHO SALMON, CHUM SALMON, SUMMER STEELHEAD, AND WHITE STURGEON

Reintroduction of Pacific lamprey in the Umatilla River in Northeast Oregon

Preliminary Summary of Out-of-Basin Steelhead Strays in the John Day River Basin

Study 9.5 Fish Distribution and Abundance in the Upper Susitna River

Transcription:

Hatchery and Supplementation Program DRAFT 2014 Report Lewis River Hydroelectric Projects FERC Project Nos. 935, 2071, 2111, 2213 Erik Lesko April 2015

TABLE OF CONTENTS 1.0 INTRODUCTION... 1 2.0 WINTER STEELHEAD... 2 2.1 Broodstock Collection... 2 2.1.1 Merwin Trap... 2 2.1.2 Tangle Netting... 4 2.1.3 Cedar Creek... 5 2.2 Winter Steelhead Broodstock Collection Timing... 5 2.3 Genetic Analysis of Potential Broodstock... 7 2.4 Genetic Analysis of BWT Steelhead Returns to the Merwin Trap... 8 2.5 Spawning and Egg Take... 9 2.6 Rearing, Tagging and Release... 11 2.6.1 Rearing... 11 2.6.2 Tagging... 12 2.6.3 Release... 12 3.0 MONITORING AND EVALUATION... 13 3.1 Winter Steelhead Redd Surveys (Lower River)... 13 3.1.1 Spawning Abundance... 13 3.1.2 Distribution... 14 3.2 Double Floy tagged steelhead returns to the Merwin Trap... 15 3.3 Ratio of NOR to HOR steelhead in the traps and tangle netting... 15 3.4 Upstream Transport of Winter Steelhead... 17 3.5 Coho and Spring Chinook Salmon Redd Surveys... 17 3.5.1 Coho and Spring Chinook Salmon 2013:... 17 3.5.2 Coho and Spring Chinook Salmon 2014:... 17 3.6 2014 Screw trap Operations... 17 3.7 Mainstem Carcass Surveys Coho and Spring Chinook Salmon... 19 3.7.1 Coho Salmon Tributary Surveys 2012:... 19 3.7.2 Coho Salmon Surveys 2013:... 19 3.7.3 Spring Chinook Surveys 2013... 19 3.7.4 Coho Salmon Surveys 2014... 19 3.7.5 Spring Chinook Surveys 2014:... 19 i

4.0 RECOMMENDATIONS FOR ONGOING MANAGEMENT... 19 5.0 REFERENCES... 20 APPENDICES Appendix A Wild Winter Steelhead Collection Log 2014 Appendix B Genetic Assignment Results from Lewis River Captures at Merwin Trap (MT), Cedar Creek (CC) and Tangle Netting (TN) 2014 Appendix C Wild Winter Steelhead Spawning Log 2014 Appendix D Wild Winter Steelhead Spawning Crosses 2014 Appendix E Steelhead Redd locations, Lewis River, WA 2014 TABLES TABLE 1. COLLECTION SUMMARY OF WINTER STEELHEAD CAPTURES BY LOCATION BETWEEN 2009 AND 2014.... 2 TABLE 2. DISPOSITION OF WINTER STEELHEAD CAPTURED AT THE MERWIN TRAP (AND LEWIS LADDER) BETWEEN JANUARY 13 AND JUNE 13, 2014.... 3 TABLE 3. DISPOSITION OF STEELHEAD CAPTURED THROUGH TANGLE NETTING IN 2014.... 4 TABLE 4. NUMBER OF SPAWNING CROSSES AND PARENTS INCLUDING THE DURATION OF EACH SPAWNING PERIODS FOR BROOD YEARS BETWEEN 2009 AND 2014.... 10 TABLE 5. SUMMARY OF REARING STATISTICS FOR THE 2014 BROOD YEAR... 11 TABLE 6. WILD WINTER STEELHEAD ABUNDANCE DOWNSTREAM OF MERWIN DAM 2008 THROUGH 2014 BASED ON REDD COUNTS.... 13 TABLE 7. REDD DISTRIBUTION SUMMARY FOR REACHES 1 THROUGH 5 INCLUDING REDDS PER MILE... 15 TABLE 8. RATIO OF HOR AND NOR STEELHEAD CAPTURED IN THE TANGLE NET FROM MARCH 1 TO MAY 12, 2014 (N=148).... 16 TABLE 9. SUMMARY OF WINTER STEELHEAD TRANSPORTED AND RELEASED UPSTREAM OF SWIFT DAM.... 17 TABLE 10. COLLECTION SUMMARY FOR THE ROTARY SCREW TRAP DEPLOYED DOWNSTREAM OF MERWIN DAM APRIL 1 JUNE 11, 2014.... 18 TABLE 11. DISPOSITION OF CAPTURED FISH (EXCLUDING FRY) INCLUDING CATCH RATES OF BWT AND NON BWT RAINBOW FORM APRIL 1 JUNE 11, 2014.... 18 ii

FIGURES Figure 1. Frequency of winter steelhead captured at the Merwin fish trap between January 1, 2014 and June 13, 2014.... 3 Figure 2. Composition of winter steelhead captured through tangle netting between February 14, 2014 and May 12, 2014 (n= 162).... 5 Figure 3. Actual collection timing of steelhead used as broodstock compared to predetermined collection curve during the 2014 season.... 7 Figure 4. Proportion of primary, secondary and tertiary genetic assignment of potential steelhead broodstock sampled in 2014 at the Merwin Trap, tangle netting and Cedar Creek Weir (n=163).... 8 Figure 5. Proportion of primary, secondary and tertiary genetic assignment of BWT steelhead transported upstream of Swift Dam from the Merwin Trap in 2014 (n=35)... 9 Figure 6. Number and timing of winter steelhead (males and females) spawned at Merwin hatchery (n=51) in 2014.... 10 Figure 7. Observed mortality rate of fish ponded for brood years 2009 through 2014... 12 Figure 8. Wild winter steelhead spawner abundance downstream of Merwin Dam 2008 through 2014 based on redd counts.... 14 iii

1.0 INTRODUCTION The purpose of this report is to document results from field studies associated with implementation of the Hatchery and Supplementation (H&S) Program during 2014. Monitoring and evaluation (M&E) activities of the H&S program are planned in consultation with the H&S subgroup and incorporated into the Annual Operating Plan (AOP) each year. In 2014, the following key activities were completed as part of the 2014 AOP: Transport of winter steelhead and early coho salmon upstream of Swift Dam Screw trapping of emigrating juveniles downstream of Merwin Dam Carcass and redd surveys downstream of Merwin Dam Broodstock collection and production for wild winter steelhead supplementation Hatchery production of trout and salmon as stipulated in Section 8 of the Lewis River Settlement Agreement. This report is required by Section 8.2.4 of the Lewis River Settlement Agreement that states: On an annual basis, the Licensees shall provide to the ACC for review and comment a report compiling all information gathered pursuant to implementation of the Hatchery and Supplementation Plan. The report also will include recommendations for ongoing management of the Hatchery and Supplementation Program. The ACC shall have 60 days to comment on the annual report. Within 60 days of the close of the comment period, the Licensees shall finalize the report after consideration of all comments. The Licensees shall also provide the comprehensive periodic review undertaken pursuant to Section 8.2.6 below to the ACC. The Licensees shall provide final annual reports and the comprehensive periodic review to the Services during the development of any required ESA permit or authorization for hatchery operations, including NOAA Fisheries HGMP process. The report may be included as part of the detailed annual reports of the ACC activities required by Section 14.2.6. 1

2.0 WINTER STEELHEAD The winter steelhead program is composed of three elements: (1) a broodstock capture program with a goal to collect up to 50 natural origin broodstock; (2) a spawning and rearing program that targets a release of 50,000 smolts each year; and finally (3) a transport program that moves all program steelhead returns upstream of Swift Dam. The primary goal of this program is to support a self sustaining population upstream of Swift Dam that in time requires no hatchery support. A summary of steelhead collected for years 2010 through 2014 is provided in Table 1. Table 1. Collection summary of winter steelhead captures by location between 2009 and 2014. YEAR 2009 2010 2011 2012 2013 2014 Merwin Trap 27 48 25 193 752 1,075 Lewis River Trap 0 0 0 7 5 1 Cedar Creek Weir 0 6 0 0 0 3 Tangle Netting 39 42 65 166 103 162 Angling 8 2 Discontinued in 2011 TOTAL 74 98 90 366 860 1,241 2.1 Broodstock Collection Broodstock collection relied principally on two methods: (1) trapping at Merwin Dam and (2) tangle netting. Cedar Creek weir contributed one broodstock and Lewis Ladder contributed one NOR that was later released. All captured NOR winter steelhead were transported to Merwin Hatchery for genetic assignment analysis. After results were known, these fish were either held for broodstock or released back to river depending on predetermined collection curves. All program fish (BWT) capured in the Merwin Trap were immediately transported upstream of Swift Dam as part of the supplementation program. Data for all steelhead transported to the Merwin hatchery is provided in Appendix A. 2.1.1 Merwin Trap The first unclipped steelhead was collected at the Merwin trap on March 23, 2014; the first BWT steelhead was captured on January 13, 2014. During the period January 13 June 13, twenty nine (29) unclipped NOR steelhead and 1,059 BWT winter steelhead were captured at the trap. Of all BWT steelhead captured, 1033 were transported upstream of which 82 BWT steelhead received a Lotek radio transmitter prior to release upstream. Of the 29 NOR s captured, 18 were spawned and 11 were released back to river. 2

Table 2. Disposition of Winter Steelhead Captured at the Merwin Trap (and Lewis Ladder) between January 13 and June 13, 2014. RETAINED Males Females Spawned 8 10 Released* 9 2 RELEASED Upriver Transport (BWT) 431 519 Upriver Transport (Radio Tagged) 20 62 NOR Kelts (to river) 4 1 Mortalities 4 4 Lewis River Ladder 1 0 TOTAL 477 598 * Released from Merwin Hatchery after genetic assignment results were known 350 Number of Steelhead 300 250 200 150 100 BWT Males BWT Females NOR's 50 0 Jan Feb Mar Apr May Jun Month NOTE: All BWT steelhead are considered HOR Figure 1. Frequency of winter steelhead captured at the Merwin fish trap between January 1, 2014 and June 13, 2014. 3

2.1.2 Tangle Netting Tangle netting efforts began on February 14, 2014 and continued through May 12, 2014. A PacifiCorp crew of two biologists went out once per week depending on river conditions. Six to eight pound test monofilament, 4 inch (stretch) mesh tangle nets were drifted in known and established steelhead holding areas. Once a steelhead became entangled in the drifting net it was pulled into the boat, freed and then placed in an insulated cooler with fresh river water. All steelhead were processed on the boat and either released or transported to an oxygenated holding tank at the Lewis River hatchery access area. Table 3 summarizes the disposition of steelhead captured during tangle netting efforts. Table 3. Disposition of steelhead captured through tangle netting in 2014. Males Females RETAINED NOR Spawned 16 16 *NOR Released (from Merwin) 15 3 HOR (Euthanized) 1 0 RELEASED (On Site) NOR kelts 23 14 HOR Kelts 0 3 BWT (double floy) 13 1 Not needed 17 0 Residuals 20 0 Recaptures 13 1 Other 4 2 Mortalities 0 0 TOTAL 122 40 * Released from Merwin Hatchery after genetic assignment results were known or received as kelts In total, 162 steelhead were captured through the tangle netting program. Of these, 112 (69%) were of natural origin (Figure 2). Remaining fish were either program returns (BWT) or from traditional hatchery programs. 4

40 HOR NOR 30 Number of Steelhead 20 10 0 Feb 14-28 Mar 1-15 Mar 16-31 Apr 1-15 Apr 16-30 May 1-12 Period Figure 2. Composition of winter steelhead captured through tangle netting between February 14, 2014 and May 12, 2014 (n= 162). 2.1.3 Cedar Creek Only three steelhead were transported from the Cedar Creek Weir to Merwin Hatchery. One fish was spawned, one fish was released back to river and the last steelhead was a mortality at the hatchery after being held for 24 days. 2.2 Winter Steelhead Broodstock Collection Timing The ability to conform to predetermined collection curves presents several difficulties in the field. Specific issues continue to make broodstock collection problematic. These specific issues include the following: More fish (than stipulated in the collection curve) need to be captured each period to ensure assignment analysis will not reduce available broodstock below target levels 5

Spawning maturity is a significant variable between males and females during spawning and adds uncertainty when deciding to retain or release broodstock. This is most notable in females rather than males. Capture efficiency is affected by river conditions which can change weekly if not daily Gender ratios need to be managed and maintained to ensure adequate number of crosses and limited multiple use of broodstock males Steelhead condition varies throughout the collection period with a larger percentage of fish being returned to river later in the collection window due to ripeness, and, Fecundity varies substantially from fish to fish and from year to year. Because of the many unknowns with collecting live fish in their natural environment, the collection curve is intended to be a guide for collection crews to help plan fish collection activities on a weekly basis. This planning helps to ensure that fish are collected across their spawning period and helps ensure genetic diversity among the available broodstock. The first steelhead retained for broodstock was on February 25, 2014 from tangle netting efforts. This fish was spawned during the first spawning event on April 7. Tangle netting efforts ended after May 12 because the broodstock collection goal had been achieved. According to the collection curve proposed in the annual plan for 2014, 50 steelhead are to be held for broodstock and spawned over the course of the run. In 2014, 51 steelhead (26 females and 25 males) were spawned. Capture timing closely matched the curve as provided in the annual plan (Figure 3) despite the challenges mentioned above. 20 18 16 Collection Curve Actual Broodstock Captures Number of Steelhead 14 12 10 8 6 4 2 0 Jan - Feb Mar 1-15 Mar 16-31 Apr 1-15 Apr 16-30 May 1-15 Collection Period 6

Figure 3. Actual collection timing of steelhead used as broodstock compared to predetermined collection curve during the 2014 season. 2.3 Genetic Analysis of Potential Broodstock The H&S Subgroup agreed to use a genetic assignment target level of 50 percent or greater to the NF Lewis River or Cedar Creek stock(s) to be considered acceptable broodstock. Additionally, steelhead captured after April 1 with 50 percent or greater assignment to the Cascade Strata are also considered acceptable broodstock. The only exception to this rule is for fish showing hatchery assignment at levels greater than 5 percent. These fish would not be incorporated into the broodstock despite any assignment of 50 percent or greater to the NF Lewis River wild winter steelhead stock or Cascade Strata. A total of 163 samples were taken from steelhead captured in the Merwin Trap, through tangle netting and the Cedar Creek Weir. All sampled steelhead were assigned a probability percentage as to likelihood of assignment to known baselines established for lower Columbia River tributaries. Probabilities are classified as primary, secondary and tertiary to account for introgression from other basins and provide a more complete picture of diversity present within the samples. Figure 4 provides an illustration of results of sampled (n=163) non BWT steelhead. Appendix B provides the tabular results for each individual unclipped steelhead captured at the Merwin trap, tangle netting and at the Cedar Creek weir. 7

0.60 NF Lewis Cedar Germany Coweeman Grays EF Lewis Mill Kalama Winter Sandy Kalama Summer SF Toutle Skamania Hatchery Green Lewis Hatchery Klickitat Summer Big Cr. Elochoman Hatchery Elochoman Kalama Hatchery Hood R. Clackamas Proportion of Samples 0.50 0.40 0.30 0.20 Primary Assignment Secondary Assignment Tertiary Assignment 0.10 0.00 Baseline Figure 4. Proportion of primary, secondary and tertiary genetic assignment of potential steelhead broodstock sampled in 2014 at the Merwin Trap, tangle netting and Cedar Creek Weir (n=163). 2.4 Genetic Analysis of BWT Steelhead Returns to the Merwin Trap Thirty five samples were obtained from BWT steelhead captured in the Merwin Trap in 2014. 1 Figure 5 presents the results from a subsample (n=35) of steelhead captured in the Merwin Trap and transported upstream of Swift Dam to spawn naturally using primary, secondary and tertiary assignment probabilities. 1 A larger sample (n > 700) of BWT steelhead captured in the Merwin Trap over multiple years is currently being analyzed to quantify family representation of BWT steelhead returns. This analysis will be presented to the ACC once finalized. 8

0.60 Merwin Cedar Mill Clackamas Germany Cr Coweeman Green R Grays EF Lewis LR Hatchery Elochoman R Elochoman H Kalama Winter SF Toutle Proportion of Samples 0.50 0.40 0.30 0.20 Primary Secondary Tertiary 0.10 0.00 Baseline Figure 5. Proportion of primary, secondary and tertiary genetic assignment of BWT steelhead transported upstream of Swift Dam from the Merwin Trap in 2014 (n=35). 2.5 Spawning and Egg Take A total of 26 females were spawned with 25 males through 26 spawning crosses (Appendix D). All fish were spawned during 26 spawning events between April 7 and May 16, 2014 (Figure 6). The target goal of 25 females and 25 males was achieved for the first time since 2009 (Table 4). 2014 also represents the largest number of pairwise crosses since the program began. However, the spawning period was very short (39 days), which is second only to 2013 in which there were only 8 total crosses. Fecundity averaged 3,849 per female. Estimated egg take (106,000) was more than the program target of 80,000 ± 20%. However, two crosses resulting in about 9,000 eggs were separated after primary assignment probabilities of the parents were within the Cascade Stratum. Eggs from these crosses were hatched and released as unfed fry into the North Fork 9

Lewis River. Appendix C provides the spawning log for 2014 indicating fecundity, crosses, eyed egg take and estimated egg loss. 6 5 Males Females Number of Spawners 4 3 2 1 0 April 7 April 11 April 15 April 21 April 24 April 25 April 29 May 2 May 9 May 16 Spawn Date Figure 6. Number and timing of winter steelhead (males and females) spawned at Merwin hatchery (n=51) in 2014. Table 4. Number of spawning crosses and parents including the duration of each spawning periods for brood years between 2009 and 2014. Brood Year Crosses Females Males Spawn Period Days 2009 21 12 19 Mar 2 May 21 80 2010 22 22 24 Mar 17 May 14 56 2011 9 16 19 Mar 30 May 18 49 2012 12 19 23 Apr 10 May 29 49 2013 8 8 11 Apr 10 May 6 26 2014 26 26 25 Apr 7 May 16 39 10

2.6 Rearing, Tagging and Release Of the 82,492 fry ponded, 11 percent (8,954) succumbed to natural mortality. Mortality caused by bacterial coldwater disease (BCW) was low at about 2 percent. Flexibacter columnaris was not observed during the rearing cycle. Past F. columnaris infections have caused significant mortality (up to 50%). Because the egg take goal was achieved and pond loss was low, the 2015 release is expected to exceed all previous releases and exceed release targets by 21,000 smolts. 2.6.1 Rearing Table 5. Summary of rearing statistics for the 2014 brood year GENERAL STATISTIC Egg Take 106,038 Eyed Eggs 93,174 Total Fry Ponded 82,492 Total Fry out planted (unfed) 9,092 MORTALITY Egg Loss 6.83% Total Pond Loss 10,903 Due to natural causes 8,954 Due to disease (Columnaris) 0 Due to disease (BCW) 1,949 Overall Loss (egg and pond) 17,734 Shortage / Adjustment 1,911 RELEASE Projected Smolt Release (survival) 71,200 Release Date (Start Volitional) May 1, 2015 Release size 8 fpp 11

100000 90000 Number of Juvenile Steelhead 80000 70000 60000 50000 40000 30000 20000 10000 0 May Jun Jul Aug Sep Oct Nov Dec Jan Feb Mar Apr 2014 2013 2012 2011 2010 2009 Month Figure 7. Observed mortality rate of fish ponded for brood years 2009 through 2014 2.6.2 Tagging All subyearling steelhead were tagged with blank wire snout tag in December 2014. In addition, a small portion (about 1,200) of the release will receive a PIT tag in the dorsal sinus and reared in circular tanks for approximately 6 to 8 weeks prior to release. This effort is a pilot study to evaluate survival of circular tank reared fish versus traditional raceway or ponding strategies. 2.6.3 Release All fish will be volitionally released on May 1, 2015 at the Merwin boat launch. Volitional release will continue until June 1, 2015. Any fish remaining in the ponds on June 1, 2015 will be 12

forced out and released downstream of the County Bridge in Woodland, WA. Projected average release size in 8 per pound. A total release number of 71,200 smolts is projected. 3.0 MONITORING AND EVALUATION 3.1 Winter Steelhead Redd Surveys (Lower River) Redd surveys are used to estimate spawning abundance and distribution of winter steelhead in the mainstem North Fork Lewis River. Surveys are conducted weekly throughout the spawning period, which typically starts March 1 and extends into mid June. 3.1.1 Spawning Abundance Spawning abundance estimates rely on new redd census data, assumed sex ratio and females per redd to calculate total spawner abundance (Freymond and Foley 1986). Females per redd follow WDFW generalized guidelines of 0.81 females per redd and sex ratio is assumed equal (Table 6). To increase precision in 2013 and 2014, the equal sex ratio assumption is compared to the observed sex ratio at the MCF. This may be a more accurate estimate of female to male ratio in the river because of the large numbers captured in the trap and is unbiased in terms of capture efficiency (Table 6). Using trap data collected for 2014, a total of 1074 steelhead were trapped. These include mainly BWT steelhead, but also include steelhead held for broodstock and steelhead released because of stubby dorsal fins and no wire tag in their snout. Of this total, 476 were male and 598 were female. Therefore, for every female we assume that there are 0.80 males (instead of 1) that make up the population. As a result, the population estimate is lower than assuming the 1:1 ratio (Table 6). The number of redds per female should also be verified at some point to improve the accuracy of this estimate. Table 6. Wild Winter Steelhead Abundance Downstream of Merwin Dam 2008 through 2014 based on redd counts. Number of Redds observed Year Spawner Observed sex ratio Estimate (females : males) 2008 131 212 2009 176 286 2010 248 402 2011 108 174 2012 343 556 2013 456 739 1 : 1.43 898 2014 364 582 1 : 0.80 531 Spawner Estimate (Corrected) 13

1000 800 Spawner Estimate Spawner Estimate (Corrected) Number of Spawners 600 400 200 0 2008 2009 2010 2011 2012 2013 2014 Year Figure 8. Wild winter steelhead spawner abundance downstream of Merwin Dam 2008 through 2014 based on redd counts. 3.1.2 Distribution A summary of redd distribution is provided in Table 7. Reach number 5 has the most redds, but also has the most habitat at 7.71 miles (not including the section downstream of Eagle Island). Redd density is highest in reach number 2 at 127 redds per mile. The relatively large number of redds in this reach is attributable to a small but heavily used spawning area approximately 0.75 miles downstream of Merwin Dam (Haggies). It is not fully understood why this area is used so extensively used by steelhead for spawning but it remains consistent from year to year. One key difference in this area is the gravel size (relatively small) and consistent flow rates along the tailout of the main pool. Redd locations are illustrated in Appendix E. Reach breaks coincide with WDFW reach breaks used for fall Chinook sampling. 14

Table 7. Redd distribution summary for reaches 1 through 5 including redds per mile Reach Reach Length (miles) Redds Redd per Mile 1 0.55 34 62 2 0.83 105 127 3 0.95 74 81 4 1.00 49 49 5 7.71 102 13 Total number of redds = 364 Average redds per mile = 33 (69 in 2013) 3.2 Double Floy tagged steelhead returns to the Merwin Trap PacifiCorp biologist double floy tagged captured blank wire tagged steelhead from tangle netting and then released them back to river. Only 14 BWT steelhead were tagged with double floy tags and released in 2014. This low number was a result of low capture rates and a majority of the fish being ripe when caught. Consequently, no double floy fish were recaptured at the MCF. One double floy tagged fish was, however, recaptured in the tangle net. This fish was first captured at Haggies on March 25, 2014 and later recaptured in the same location on April 15, 2014. The 670 mm male had lost one of the floy tags and was ripe when recaptured. It is assumed that this male spawned at Haggies. Given the low tagging and recapture rate it is not possible to make conclusions regarding recruitment to the trap or presence of BWT steelhead on the spawning grounds using this method. 3.3 Ratio of NOR to HOR steelhead in the traps and tangle netting Program returns are treated as hatchery origin (HOR) steelhead despite their genotype assignment to NOR stocks. This is due to the hatchery influence during mating and captive rearing conditions during their first year of life. As these program fish return as adults, there is opportunity for these (HOR) fish to spawn with NOR stocks. It has been shown that reproductive success (fitness) declines rapidly (up to 37 percent per captive reared generation) within a natural population (Araki et. al. 2007). The evolutionary mechanisms for declines in fitness are not fully understood, but hatchery protected rearing environments and controlled mating selection are suspected contributors to this decline (Araki et. al 2007). Inbreeding between program fish is also a concern because of loss in genetic diversity or effective population size further limits fitness and adaptability of the natural spawning population. In 2014, the ratio of HOR to NOR captured through tangle netting is used to estimate the potential influence of HOR on the NOR population. Tangle netting provides a direct measure of the proportion of hatchery to natural origin fish on the spawning grounds when done during 15

spawning and in areas where spawning is occurring. Figure 1 and 2 provides the total number of HOR and NOR s captured in both the MCF and through tangle netting. The MCF, however, should not be used to estimate phos as it is biased towards BWT steelhead because Merwin hatchery effluent water is used to supplement attraction water for the trap. To estimate phos through tangle netting, the number of HOR (includes BWT) and NOR are recorded during the natural spawn time of NOR winter steelhead in the Lewis River (March June). The total HOR and NOR captures during this period provides an estimate of the ratio present on the spawning grounds (Table 8). The use of tangle netting is limited by its duration in that netting normally does not extend past the first or second week of May. Netting past this date raises the potential for disrupting and perhaps reducing natural spawning success, because nearly all steelhead captured past this date are either kelts or active spawners. Despite this limitation, tangle netting remains the only means at this time to directly sample and determine origin of spawning steelhead for estimating phos. Table 8. Ratio of HOR and NOR steelhead captured in the tangle net from March 1 to May 12, 2014 (n=148). Total HOR 43* Total NOR 105 phos 29 % phos (excluding BWT) 3 % Males Females HOR NOR HOR NOR 29 81 14 24 * Total includes 40 BWT and 3 AD clipped winter steelhead Based on tangle net captures during the spawning period, we estimate the proportion of hatchery origin spawners is 29 percent. The main contributor to this estimate is the number of BWT steelhead on the spawning grounds during the natural spawning period. If BWT steelhead are excluded and only AD clipped steelhead are included, phos is reduced 26 percent to only 3 percent, which exceeds HSRG targets for integrated programs. The ability to remove a meaningful number of BWT and AD clipped winter steelhead spawning in the lower river would be challenging without directly and irreversibly reducing NOR spawning success. 16

3.4 Upstream Transport of Winter Steelhead In 2014, a total of 1033 blank wire tagged steelhead were transported upstream of Swift Dam (Table 9). A total of 82 transported steelhead also received a surgically implanted radio transmitter for distribution studies in the upper basin. Distribution and detection data along biological data each of these fish is provided in the Aquatic Monitoring and Evaluation Report as an appendix to the ACC/TCC annual report. This year represents the third year of transportation activities and numbers continue to increase each year. The goal of the H&S program is 500 winter steelhead transported each year. We have exceeded this target the last two years and more than doubled the target in 2014 (Table 9). Table 9. Summary of Winter Steelhead transported and released upstream of Swift Dam. YEAR Number Number fitted with radio Transported transmitters 2012 189 39 2013 741 100 2014 1033 82 3.5 Coho and Spring Chinook Salmon Redd Surveys 3.5.1 Coho and Spring Chinook Salmon 2013: Meridian to provide 3.5.2 Coho and Spring Chinook Salmon 2014: Meridian to provide 3.6 2014 Screw trap Operations As in 2013, the 2014 screw trapping effort was conducted near the golf course using one 8 foot diameter screw trap from April 1 through June 11, 2014. In total, 23,279 fish were captured (3,011 smolts) in the screw trap during the collection period (Table 10). Of these, 2,828 smolts were marked and transported upstream for recapture and trap efficiency testing (Table 11). Only 29 recaptures were reported from the marked release groups. Despite improvements in capture efficiency in 2014 (1.03%) as compared to 2013 (0.60%), trapping efficiency remains low for calculating abundance with acceptable confidence. To help compensate for low trapping efficiencies, the trapping crew marked and released all smolts captured in the screw trap (2,828) upstream for efficiency testing. 17

Meridian to provide analysis Table 10. Collection summary for the rotary screw trap deployed downstream of Merwin Dam April 1 June 11, 2014. Species Life Stage No Collected Period of collection Mean Length (FL, mm) MIN (mm) MAX (mm) σ Coho Rainbow Chinook Cutthroat Chum Lamprey TOTAL Table 11. Disposition of captured fish (excluding fry) including catch rates of BWT and non BWT rainbow form April 1 June 11, 2014. Total Released upstream Rainbow Coho Cutthroat Recaptures 29 (1.03%) Total Released Downstream Rainbow Cutthroat Coho Non Clipped Rainbow BWT Non BWT 18

3.7 Mainstem Carcass Surveys Coho and Spring Chinook Salmon *NOTE: Mainstem and tributary carcass survey data for spring Chinook and coho are surveyed beginning in the fall season of each year and extend until the end of January. This schedule does not typically provide adequate time to input, review and analyze collected data and present the results in formal reporting by April 1 of each year. Therefore, reporting for mainstem and tributary surveys of coho and spring Chinook are delayed one year from the survey period. For example, survey data collected in the fall of 2013 (and early 2014) are not reported until the 2014 annual operations report is finalized and submitted to the FERC in April of 2015. There may also be some instances where more information is needed before final results are presented which further delays reporting. However, placeholders titles in each report iteration are never removed until the data are available, analyzed or deemed inadequate. 3.7.1 Coho Salmon Tributary Surveys 2012: WDFW to provide results 3.7.2 Coho Salmon Surveys 2013: Meridian to provide results. 3.7.3 Spring Chinook Surveys 2013: Meridian to provide results 3.7.4 Coho Salmon Surveys 2014: Meridian to provide results 3.7.5 Spring Chinook Surveys 2014: Meridian to provide results 4.0 RECOMMENDATIONS FOR ONGOING MANAGEMENT Improvements in the way we handle and rear steelhead from wild winter broodstock continues to improve. Noticeable declines in mortality of both adults and rearing juveniles has declined from previous years. Also, rearing conditions have improved as recommendations from the H&S subgroup have been implemented. The pilot test use of circular tanks (as opposed to ponds) will continue for the 2014 brood year to determine if survival improves as is supported by several studies. If results confirm benefits to survival (SAR) the use of circular tank rearing may be expanded to include a larger portion of the smolt releases. Developing estimates of abundance for juveniles that meet precision goals continues to be a challenge. Annual planning meetings need to focus on developing standardized sampling 19

methods that strives to meet NOAA precision guidelines for monitoring fish populations. Our trapping efforts for estimating juvenile abundance currently does not achieve collection efficiencies needed to calculate precise estimates of abundance. In 2015, the placement of an additional trap may improve trap efficiency to a level that allows more precise estimators of abundance. In addition to monitoring methods, the H&S Subgroup should focus on the effects of the late winter broodstock program on NOR winter steelhead stocks in the lower river. The effects of mining and program returns need closer evaluation. While the program is temporary, there may be long term genetic impacts to the locally adapted population that may not yet be realized. These potential impacts should be monitored with methods that are now available to quantify metrics such as effective population size and inbreeding coefficients. These metrics are consistent with regional best management practices and should be developed further for the North Fork Lewis River late winter steelhead program. 5.0 REFERENCES Araki, H., Cooper, B., and S. Blouin. 2007. Genetic Effects of Captive Breeding Cause a Rapid, Cumulative Fitness Decline in the Wild. Science, vol. 318, Octber 5, 2007. Freymond, B., and S. Foley. 1986. Wild steelhead spawning escapement estimates from Boldt Case rivers 1985. Washington Department of Game. Fish Management Division. Rep. No. 86 12. Olympia,WA. Hilborn, R., B. G. Bue, and S. Sharr. 1999. Estimating spawning escapement for periodic counts: a comparison of methods. Canadian Journal of Fisheries and Aquatic Sciences 56:888 896 Hill, R. A. 1997. Optimizing aerial count frequency for area under the curve method of estimating escapement. North American Journal of Fisheries Management 17:461 466. Hood, G. M. 2009. Pop Tools version 3.1.0. URL http://www.cse.csiro.au/poptools. Kinsel, C., P. Hanratty, M. Zimmerman, B. Glaser, S. Gray, T. Hillson, D. Rawding, and S. VanderPloeg. 2009. Intensively Monitored Watersheds: 2008 Fish Population Studies in the Hood Canal and Lower Columbia Stream Complexes. FPA 09 12, Washington Department of Fish and Wildlife, Olympia, Washington. 20

Appendix A Wild Winter Steelhead Collection Log 2014

CAPTURE DATE CAPTURE METHOD Gender Fork Length (cm) Pit Tag # DNA Sample # Genetic Probability Assignment Comments 2/21/2014 Tangle Net M 72 A0A0 TN14 1 LRMerwin.3464 2/25/2014 Tangle Net M 93 A05E TN14 2 LRMerwin.9721 3/4/2014 Cedar F 76 3D91C2DD5601E CC14 14 LRMerwin.1638 3/4/2014 Cedar M 68 3D91C2DD678B CC14 20 LRMerwin.7812 3/4/2014 Cedar M 71 3D91C2DD51559 CC 21 LRCedar.7514 Mortality 3/28/14 3/11/2014 Tangle Net M 78 0EE0 TN14 3 LRMerwin.1814 3/11/2014 Tangle Net F 80 0F1A TN14 4 LRMerwin.6138 3/11/2014 Tangle Net M 74 0F1F TN14 5 LRMerwin.2145 3/11/2014 Tangle Net M 82 0E87 TN14 6 LRMerwin.6853 3/11/2014 Tangle Net M 87 0E5F TN14 7 LRMerwin.9644 To Many Males / Returned to river Tangle Net 3/11/2014 Tangle Net M 61 0F28 TN14 8 LRMerwin.8317 To Many Males / Returned to river Tangle Net 3/11/2014 Tangle Net M 68 A086 TN14 9 LRMerwin.7792 To Many Males / Returned to river Tangle Net 3/11/2014 Tangle Net M 71 9E8D TN14 10 LRMerwin.9484 To Many Males / Returned to river Tangle Net 3/17/2014 Tangle Net M 78 A07F TN14 11 LRCedar.4302 3/17/2014 Tangle Net F 71 A0A3 TN14 12 LRCedar.4188 3/23/2014 Merwin Trap F 73 A20D MT14 1 LRMerwin.3813 3/25/2014 Tangle Net F 69 A0B3 TN14 13 LRCedar.5232 3/25/2014 Tangle Net M 95 A092 TN14 14 LRCedar.4223 3/25/2014 Tangle Net F 66 A068 TN14 15 LRMerwin.9928 3/25/2014 Tangle Net M 71 A057 TN14 16 LRCedar.2075 3/26/2014 Merwin Trap F 71 A1FB MT14 2 LRMerwin.909 3/27/2014 Merwin Trap M 91 A226 MT14 3 LRMerwin.9068 4/1/2014 Merwin Trap M 65 A1FA MT14 4 LRCedar.8996 4/1/2014 Tangle Net F 63 0F08 TN 14 17 LRMerwin.9488 4/1/2014 Tangle Net F 79 0F45 TN 14 18 LRMerwin.9299 4/2/2014 Merwin Trap M 81 A22C MT 14 5 LRMerwin.9088 4/4/2014 Tangle Net M 67 0E40 TN 14 19 LRMerwin.7458 4/4/2014 Tangle Net M 74 0F30 TN 14 20 LRCedar.019 4/4/2014 Tangle Net M 96 0EC9 TN 14 21 LRMerwin.9969 4/4/2014 Tangle Net M 79 0E9C TN 14 22 LRMerwin.4237 4/8/2014 Tangle Net F 67 0E42 TN 14 29 LRMerwin.5953 Genetic sample mixed w/ 264 4/8/2014 Tangle Net F 73 0E91 TN 14 30 LRCedar.9081 Genetic sample mixed w/ 265 4/8/2014 Tangle Net F 63 0EFD TN 14 31 LRCedar.5404 Genetic sample mixed w/ 266 4/8/2014 Tangle Net F 72 0EAD TN 14 32 LRMerwin.9264 4/8/2014 Tangle Net F 72 0EF9 TN 14 41 LRMerwin.7531 4/8/2014 Tangle Net M 80 0E99 TN 14 43 LRMerwin.985 4/8/2014 Tangle Net F 71 0ED8 TN 14 47 LR Cedar.4531 Skipped Red vials 268 270 4/21/2014 Merwin Trap F 79 0957 MT 14 6 Mill Cr..5049 URT 14 309. Unfed fry plant 4/22/2014 Tangle Net F 77 0F04 TN 14 56 LRMerwin.7704 APPENDIX A 1

CAPTURE DATE CAPTURE METHOD Gender Fork Length (cm) Pit Tag # DNA Sample # Genetic Probability Assignment 4/22/2014 Tangle Net F 74 0E90 TN 14 57 LRMerwin.1412 4/22/2014 Tangle Net M 64 0EDF TN 14 59 LRMerwin.5357 4/24/2014 Merwin Trap F 71 A246 MT 14 7 LRCedar.6874 4/24/2014 Merwin Trap F 67 A1FD MT 14 8 LRCedar.5262 4/25/2014 Merwin Trap M 95 A243 MT 14 9 LRMerwin.9349 4/25/2014 Tangle Net F 81 0E8A TN 14 60 LRMerwin.8184 4/25/2014 Tangle Net M 61 0F4A TN 14 61 LRCedar.8177 Spawned Out 4/25/2014 Tangle Net M 77 0F2B TN 14 62 LRMerwin.6964 4/29/2014 Tangle Net M 73 0EE4 TN 14 63 LRMerwin.8515 4/29/2014 Tangle Net M 75 0EEE TN 14 64 ElochHat.2938 4/29/2014 Tangle Net M 72 6A36 TN 14 65 LRMerwin.9094 4/29/2014 Tangle Net M 89 6A44 TN 14 66 LRMerwin.8872 MORTALITY 4/30 4/29/2014 Tangle Net F 66 6A4F TN 14 67 EFLewis.7147 4/29/2014 Tangle Net M 71 6A46 TN 14 68 LRCedar.4853 4/29/2014 Tangle Net F 76 6A23 TN 14 69 LRMerwin.9712 4/29/2014 Tangle Net M 79 6A24 TN 14 70 SandyR.6495 4/29/2014 Tangle Net M 47 6A34 TN 14 71 LRMerwin.9836 5/2/2014 Merwin Trap F 83 A235 MT 14 10 Grays R..498 Unfed Fry Plant Comments 5/3/2014 Merwin Trap M 76 A239 MT 14 11 LRMerwin.496 5/6/2014 Merwin Trap M 77 A23E MT 14 12 LRCedar.5175 5/6/2014 Merwin Trap F 70 A213 MT 14 13 LRMerwin.6656 5/6/2014 Merwin Trap M 86 A229 MT 14 14 LRMerwin.9826 Spawned Out 5/6/2014 Merwin Trap M 86 A1F7 MT 14 15 LRMerwin.9817 5/6/2014 Tangle Net M 89 6A31 TN 14 73 LRMerwin.6858 5/6/2014 Tangle Net M 74 6A45 TN 14 74 GraysR..8415 5/6/2014 Tangle Net F 75 6A70 TN 14 77 LRMerwin.6625 5/6/2014 Tangle Net F 71 6A6E TN 14 78 LRMerwin.763 5/7/2014 Merwin Trap F 73 A208 MT 14 16 LRMerwin.9945 5/8/2014 Merwin Trap M 75 A21F MT 14 17 LRMerwin.9402 5/8/2014 Merwin Trap F 74 A216 MT 14 18 LRMerwin.6908 5/12/2014 Merwin Trap F 70 A207 MT 14 19 LRMerwin.235 no assigned males on hand 5/12/2014 Merwin Trap F 72 A1EB MT 14 20 LRMerwin.8029 no assigned males on hand 5/12/2014 Merwin Trap F 67 A234 MT 14 21 LRMerwin.9341 5/12/2014 Tangle Net M 77 6A65 TN 14 34 LRCedar.567 5/12/2014 Tangle Net M 73 6A22 TN 13 41 LRCedar.3651 5/14/2014 Lewis M 77 A20A MT 14 22 Lewis River Ladder APPENDIX A 2

APPENDIX B: Genetic Assignment Results from Lewis River Captures at Merwin Trap (MT), Cedar Creek (CC) and Tangle Netting (TN) 2014

DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 03/07/14 cc14 01 LRMerwin 0.668 LRCedar 0.1513 KalamSu 0.0569 KalamW 0.0492 GarysR 0.0425 03/07/14 cc14 02 GermCr 0.8903 MillCr 0.0272 KalamW 0.0236 SFTout 0.0231 ElochHat 0.0148 03/07/14 cc14 03 MillCr 0.9607 GermCr 0.0245 GarysR 0.0117 03/07/14 cc14 04 LRCedar 0.7277 LRhatW 0.2514 LRMerwin 0.0067 ElochHat 0.0062 03/07/14 cc14 06 KlickSu 0.8241 MillCr 0.0751 Cowman 0.0495 ElochHat 0.0406 LRhatW 0.006 03/07/14 cc14 07 SFTout 0.4878 LRCedar 0.3142 LRMerwin 0.1131 GrRLC 0.0469 KalamW 0.0172 03/07/14 cc14 08 GermCr 0.6567 LRCedar 0.2591 LRMerwin 0.048 KalamSu 0.0206 GarysR 0.007 03/07/14 cc14 09 LRCedar 0.7165 KalamSu 0.1115 LRhatW 0.0967 GermCr 0.0433 GrRLC 0.0187 03/07/14 cc14 10 LRCedar 0.3292 MillCr 0.1639 GermCr 0.1424 LRMerwin 0.1288 SFTout 0.1128 03/07/14 cc14 11 LRMerwin 0.5494 LRCedar 0.4386 ElochHat 0.0035 03/07/14 cc14 12 GarysR 0.5557 LRCedar 0.3846 LRMerwin 0.034 Cowman 0.0091 GrRLC 0.0071 03/07/14 cc14 13 EFLewisR 0.1785 LRCedar 0.1734 Cowman 0.1334 GermCr 0.1091 KalamW 0.099 03/07/14 cc14 14 SandyR 0.266 GarysR 0.2111 LRMerwin 0.1638 Cowman 0.1125 GermCr 0.1116 03/07/14 cc14 15 LRCedar 0.4417 ElochHat 0.2921 Cowman 0.2549 GermCr 0.0057 03/07/14 cc14 16 LRhatW 0.5747 LRCedar 0.2004 LRMerwin 0.0529 EFLewisR 0.0526 Cowman 0.0519 03/07/14 cc14 17 LRCedar 0.9034 ElochHat 0.0175 KalamW 0.017 LRMerwin 0.0141 Cowman 0.0125 03/07/14 cc14 18 Cowman 0.8844 LRMerwin 0.0734 LRCedar 0.0391 03/07/14 cc14 19 GarysR 0.4867 LRhatW 0.2009 GermCr 0.1728 Cowman 0.0824 LRCedar 0.0398 03/07/14 cc14 20 LRMerwin 0.7812 LRCedar 0.1385 Cowman 0.0385 KalamW 0.0282 KalamSu 0.0058 03/07/14 cc14 21 LRCedar 0.7514 LRMerwin 0.1541 MillCr 0.0292 GarysR 0.0255 Cowman 0.0225 03/31/14 CC14 22 LRCedar 0.8183 Cowman 0.0877 LRMerwin 0.0419 SandyR 0.0173 ElochR 0.0122 03/31/14 CC14 23 LRMerwin 0.3962 HoodR 0.3224 LRCedar 0.2638 Cowman 0.0079 03/31/14 CC14 24 LRCedar 0.7087 ElochHat 0.2874 03/31/14 CC14 25 LRCedar 0.6221 Cowman 0.1425 LRhatW 0.0515 GrRLC 0.0306 KalamW 0.0302 03/31/14 CC14 26 Cowman 0.3149 MillCr 0.2882 LRCedar 0.2332 LRMerwin 0.1118 ElochHat 0.0355 03/31/14 CC14 27 Cowman 0.6715 LRCedar 0.1803 LRMerwin 0.1423 03/31/14 CC14 28 LRMerwin 0.5873 LRCedar 0.3593 KalamW 0.0356 MillCr 0.0046 Cowman 0.0043 03/31/14 CC14 29 LRMerwin 0.9907 03/31/14 CC14 30 LRCedar 0.4137 GermCr 0.3861 LRMerwin 0.0962 GrRLC 0.0826 Cowman 0.0099 05/21/14 cc14 31 LRMerwin 0.8979 LRCedar 0.0686 GermCr 0.0136 Cowman 0.0119 05/21/14 cc14 32 LRCedar 0.6329 LRMerwin 0.3325 Cowman 0.0151 LRhatW 0.0144 APPENDIX B 1

DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 05/21/14 cc14 33 KalamW 0.6672 GermCr 0.1061 SandyR 0.0741 GarysR 0.0401 EFLewisR 0.0349 05/21/14 cc1434 LRCedar 0.6624 MillCr 0.1322 GermCr 0.0823 LRMerwin 0.0727 Cowman 0.0367 05/21/14 cc1435 BigCr 0.6431 LRMerwin 0.2688 GermCr 0.0268 LRCedar 0.0239 MillCr 0.0185 05/21/14 cc1436 LRMerwin 0.5403 LRCedar 0.4205 KalamW 0.0311 05/21/14 cc1437 LRCedar 0.9608 EFLewisR 0.0152 KalamW 0.01 LRMerwin 0.0053 05/21/14 cc1438 LRMerwin 0.9065 LRCedar 0.0687 KalamW 0.0202 05/21/14 cc1439 LRMerwin 0.3951 LRCedar 0.3364 Cowman 0.1777 GrRLC 0.0717 GarysR 0.01 05/21/14 cc14 40 LRCedar 0.6916 LRMerwin 0.2173 GermCr 0.0274 SandyR 0.0256 GrRLC 0.0152 05/21/14 cc14 41 LRMerwin 0.3803 Cowman 0.3323 GermCr 0.133 LRCedar 0.1292 MillCr 0.0134 05/21/14 cc14 42 LRCedar 0.6648 MillCr 0.0961 GermCr 0.0808 LRMerwin 0.0775 KalamW 0.0382 05/21/14 cc14 43 GermCr 0.5818 GrRLC 0.3573 LRCedar 0.021 GarysR 0.0187 BigCr 0.0062 05/21/14 cc14 44 LRCedar 0.304 LRMerwin 0.2497 Cowman 0.1596 KalamW 0.153 LRhatW 0.1175 05/21/14 cc14 45 LRMerwin 0.9922 05/21/14 cc14 46 LRMerwin 0.985 GermCr 0.0117 05/21/14 CC14 47 LRCedar 0.2924 MillCr 0.269 LRMerwin 0.2682 GermCr 0.1208 Cowman 0.027 05/16/14 CC14 48 MillCr 0.3746 KalamW 0.3156 KalamSu 0.0837 GermCr 0.0647 LRCedar 0.0575 05/21/14 CC14 48 KalamW 0.4458 MillCr 0.3063 GermCr 0.0574 KalamSu 0.0545 LRCedar 0.049 05/16/14 CC14 49 LRCedar 0.7345 LRMerwin 0.2587 05/21/14 CC14 49 LRCedar 0.736 LRMerwin 0.2561 05/16/14 CC14 50 SkamHat 0.7349 GermCr 0.1276 KalamSu 0.0524 LRMerwin 0.0364 EFLewisR 0.0242 05/21/14 CC14 50 SkamHat 0.7301 GermCr 0.1396 KalamSu 0.0421 LRMerwin 0.0378 EFLewisR 0.0238 05/16/14 CC14 51 LRCedar 0.5433 LRMerwin 0.2731 Cowman 0.0636 GarysR 0.0572 MillCr 0.032 05/21/14 CC14 51 LRCedar 0.5484 LRMerwin 0.2724 Cowman 0.0661 GarysR 0.0524 MillCr 0.031 05/02/14 MT14 07 LRCedar 0.6874 LRMerwin 0.3089 05/02/14 MT14 08 LRCedar 0.5262 LRMerwin 0.4588 GarysR 0.004 SandyR 0.003 05/02/14 MT14 09 LRMerwin 0.9349 LRCedar 0.0279 Cowman 0.0158 EFLewisR 0.0132 03/31/14 MT14 1 GrRLC 0.4805 LRMerwin 0.3813 LRCedar 0.1043 MillCr 0.0225 ElochR 0.0051 05/09/14 MT14 10 GarysR 0.498 LRCedar 0.1987 GermCr 0.1969 SandyR 0.0701 KalamSu 0.0221 05/09/14 MT14 11 LRMerwin 0.496 LRCedar 0.2467 GarysR 0.1442 GermCr 0.1076 05/09/14 MT14 12 LRCedar 0.5175 LRMerwin 0.4637 Cowman 0.013 05/09/14 MT14 13 LRMerwin 0.6656 Cowman 0.2276 LRCedar 0.101 APPENDIX B 2

DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 05/09/14 MT14 14 LRMerwin 0.9826 GrRLC 0.0121 05/09/14 MT14 15 LRMerwin 0.9817 LRCedar 0.0175 05/09/14 MT14 15 LRMerwin 0.9963 05/16/14 MT14 16 LRMerwin 0.9945 05/16/14 MT14 17 LRMerwin 0.9402 LRCedar 0.0355 KalamW 0.0144 05/16/14 MT14 18 LRMerwin 0.6908 LRCedar 0.223 KalamW 0.0555 Cowman 0.0141 EFLewisR 0.0098 05/16/14 MT14 19 GermCr 0.4831 LRMerwin 0.235 KalamW 0.1845 Cowman 0.0669 ElochHat 0.01 04/05/14 MT14 2 LRMerwin 0.909 LRCedar 0.0825 05/16/14 MT14 20 LRMerwin 0.8029 LRCedar 0.1386 Cowman 0.0184 ElochR 0.012 GarysR 0.0107 05/16/14 MT14 21 LRMerwin 0.9341 LRCedar 0.0586 04/05/14 MT14 3 LRMerwin 0.9068 LRCedar 0.0813 Cowman 0.0119 04/05/14 MT14 4 LRCedar 0.8996 GrRLC 0.0594 KalamW 0.0249 Cowman 0.0131 04/11/14 MT14 5 LRMerwin 0.9088 LRCedar 0.0471 KalamW 0.0229 Cowman 0.0109 GrRLC 0.0066 05/16/14 TN13 32 Cowman 0.922 LRCedar 0.0406 LRMerwin 0.0344 05/16/14 TN13 33 LRMerwin 0.9507 GermCr 0.0227 LRCedar 0.0122 GarysR 0.0055 05/16/14 TN13 34 LRCedar 0.567 LRMerwin 0.3762 Cowman 0.0374 KalamW 0.0098 05/16/14 TN13 35 LRCedar 0.5412 GarysR 0.2009 Cowman 0.1869 ElochR 0.0253 LRMerwin 0.0217 05/16/14 TN13 36 LRMerwin 0.7684 LRCedar 0.225 05/16/14 TN13 37 LRCedar 0.5489 LRMerwin 0.2837 KalamW 0.0645 KalamSu 0.0355 GermCr 0.0238 05/16/14 TN13 38 LRMerwin 0.8637 LRCedar 0.1299 05/16/14 TN13 39 LRCedar 0.5162 LRMerwin 0.4645 EFLewisR 0.0058 GermCr 0.0032 Cowman 0.0026 05/16/14 TN13 40 LRMerwin 0.983 GermCr 0.0141 05/16/14 TN13 41 LRCedar 0.3651 LRMerwin 0.2634 KalamW 0.224 KalamSu 0.0736 GarysR 0.0168 05/16/14 TN13 42 LRMerwin 0.4399 Cowman 0.4159 GermCr 0.0527 LRCedar 0.0402 GrRLC 0.0174 05/16/14 TN13 43 LRMerwin 0.6698 LRCedar 0.2341 EFLewisR 0.0837 KalamW 0.0041 05/02/14 TN13 61 LRCedar 0.8177 GermCr 0.1349 EFLewisR 0.0309 GrRLC 0.0046 KalamW 0.0042 05/02/14 TN13 62 LRMerwin 0.6964 GermCr 0.2404 LRCedar 0.0588 05/02/14 TN13 63 LRMerwin 0.8515 LRCedar 0.1366 Clack 0.0074 05/02/14 TN13 64 LRCedar 0.5445 ElochHat 0.2938 LRMerwin 0.0949 GermCr 0.0358 Cowman 0.0244 05/02/14 TN13 65 LRMerwin 0.9094 LRCedar 0.0638 Cowman 0.0161 EFLewisR 0.0061 05/02/14 TN13 66 LRMerwin 0.8872 LRCedar 0.038 SandyR 0.0354 EFLewisR 0.0165 GermCr 0.0101 APPENDIX B 3

DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 05/02/14 TN13 67 EFLewisR 0.7147 KalamSu 0.143 LRMerwin 0.1223 SandyR 0.0063 GermCr 0.0058 05/02/14 TN13 68 LRCedar 0.4853 LRMerwin 0.4728 Cowman 0.0162 GermCr 0.0149 GrRLC 0.0104 05/02/14 TN13 69 LRMerwin 0.9712 LRCedar 0.0268 05/02/14 TN13 70 SandyR 0.6495 LRMerwin 0.2804 GrRLC 0.0394 LRCedar 0.0247 05/02/14 TN13 71 LRMerwin 0.9836 LRCedar 0.0137 05/09/14 TN13 72 LRMerwin 0.6076 LRCedar 0.3543 GrRLC 0.0207 KalamSu 0.0072 KalamW 0.0053 05/09/14 TN13 73 LRMerwin 0.6858 LRCedar 0.2403 GermCr 0.0577 KalamW 0.007 05/09/14 TN13 74 GarysR 0.8415 GrRLC 0.0607 GermCr 0.0524 MillCr 0.0255 LRMerwin 0.0124 05/09/14 TN13 75 LRCedar 0.8541 GarysR 0.0947 LRMerwin 0.0292 KalamSu 0.0164 05/09/14 TN13 76 LRMerwin 0.997 05/09/14 TN13 77 LRMerwin 0.6625 LRCedar 0.3059 GrRLC 0.0284 05/09/14 TN13 78 LRMerwin 0.763 LRCedar 0.2302 02/21/14 TN14 1 LRMerwin 0.3464 GrRLC* 0.2829 KalamW 0.1402 Cowman 0.1052 KalamSu 0.0736 02/25/14 TN14 2 LRMerwin 0.9721 LRCedar 0.0228 03/14/14 TN14 3 KalamSu 0.214 LRMerwin 0.1814 EFLewisR 0.1808 GrRLC 0.1359 KalamW 0.1093 03/14/14 TN14 4 LRMerwin 0.6138 LRCedar 0.3254 GermCr 0.0186 KalamW 0.0152 GarysR 0.011 03/14/14 TN14 5 LRCedar 0.3559 GermCr 0.3343 LRMerwin 0.2145 KalamW 0.0416 GarysR 0.0365 03/14/14 TN14 6 LRMerwin 0.6853 LRCedar 0.2477 KalamW 0.0428 Cowman 0.0181 03/14/14 TN14 7 LRMerwin 0.9644 LRCedar 0.033 03/14/14 TN14 8 LRMerwin 0.8317 KalamW 0.1027 GermCr 0.0574 03/14/14 TN14 9 LRMerwin 0.7792 LRCedar 0.1844 SandyR 0.0199 EFLewisR 0.0047 GermCr 0.0047 03/14/14 TN14 10 LRMerwin 0.9484 LRCedar 0.0435 03/20/14 TN14 11 LRCedar 0.4302 LRMerwin 0.3456 KalamW 0.093 GarysR 0.0729 Cowman 0.0307 03/20/14 TN14 12 SFTout 0.483 LRCedar 0.4188 Cowman 0.027 KalamW 0.0256 SandyR 0.0212 03/31/14 TN14 13 LRCedar 0.5232 LRMerwin 0.1899 Cowman 0.1606 KalamSu 0.0459 GermCr 0.0299 03/31/14 TN14 14 LRCedar 0.4223 MillCr 0.3349 SandyR 0.1622 GermCr 0.0351 ElochR 0.0342 03/31/14 TN14 15 LRMerwin 0.9928 03/31/14 TN14 16 GermCr 0.5877 LRCedar 0.2075 KalamSu 0.1041 EFLewisR 0.0635 SandyR 0.0172 04/05/14 TN14 17 LRMerwin 0.9488 LRCedar 0.0304 GermCr 0.009 Cowman 0.0065 04/05/14 TN14 18 LRMerwin 0.9299 KalamW 0.044 GrRLC 0.0187 04/11/14 TN14 19 LRMerwin 0.7458 LRCedar 0.2332 Cowman 0.0157 APPENDIX B 4

DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 04/11/14 TN14 20 GermCr 0.8076 ElochR 0.1244 GarysR 0.0221 LRCedar 0.019 MillCr 0.015 04/11/14 TN14 21 LRMerwin 0.9969 04/11/14 TN14 22 LRMerwin 0.4237 LRCedar 0.3772 GermCr 0.0756 EFLewisR 0.0358 Cowman 0.025 04/11/14 TN14 23 LRMerwin 0.9943 04/11/14 TN14 24 LRMerwin 0.5408 Cowman 0.3557 KalamSu 0.0589 LRCedar 0.0349 04/11/14 TN14 25 LRCedar 0.6158 LRMerwin 0.3407 Cowman 0.0389 04/11/14 TN14 26 LRMerwin 0.4221 LRCedar 0.2714 LRhatW 0.2641 ElochHat 0.0289 Cowman 0.005 04/11/14 TN14 27 LRMerwin 0.5268 LRCedar 0.4331 GrRLC 0.03 KalamW 0.004 04/11/14 TN14 28 LRMerwin 0.9808 LRCedar 0.0185 04/11/14 TN14 29 LRMerwin 0.5953 GermCr 0.2219 LRCedar 0.1232 SandyR 0.0387 ElochR 0.0111 04/11/14 TN14 30 LRCedar 0.9081 LRMerwin 0.0502 GermCr 0.0106 Cowman 0.0093 ElochHat 0.0091 04/11/14 TN14 31 LRCedar 0.5404 LRMerwin 0.359 GermCr 0.024 KalamW 0.0223 EFLewisR 0.0191 04/11/14 TN14 32 LRMerwin 0.9264 LRCedar 0.0717 04/18/14 TN14 33 LRMerwin 0.5484 LRCedar 0.4038 Cowman 0.0474 04/18/14 TN14 34 LRCedar 0.6692 Cowman 0.2483 LRMerwin 0.0663 ElochHat 0.0117 04/18/14 TN14 35 LRMerwin 0.8074 LRCedar 0.1151 Cowman 0.0621 KalamSu 0.009 04/18/14 TN14 36 GermCr 0.5193 KalmFHat 0.3381 Cowman 0.0549 LRMerwin 0.0454 LRCedar 0.0279 04/18/14 TN14 38 LRMerwin 0.9359 GrRLC 0.0456 LRCedar 0.0059 KalamW 0.0043 04/18/14 TN14 39 LRCedar 0.899 KalamW 0.0473 GarysR 0.0203 LRMerwin 0.0133 KalamSu 0.0116 04/18/14 TN14 40 LRMerwin 0.5144 LRCedar 0.4419 GermCr 0.0212 KalamW 0.0042 EFLewisR 0.0041 04/18/14 TN14 41 LRMerwin 0.7531 LRCedar 0.2253 KalamW 0.0075 Cowman 0.0065 04/18/14 TN14 42 LRCedar 0.9183 ElochHat 0.048 LRMerwin 0.0284 04/18/14 TN14 43 LRMerwin 0.985 GarysR 0.0059 04/18/14 TN14 44 LRMerwin 0.891 LRCedar 0.0809 GermCr 0.0143 SandyR 0.0105 04/18/14 TN14 45 LRMerwin 0.6593 LRCedar 0.2761 ElochR 0.0326 GarysR 0.0119 GermCr 0.0093 04/18/14 TN14 46 LRCedar 0.7831 LRMerwin 0.2073 04/18/14 TN14 47 LRCedar 0.4531 LRMerwin 0.3688 ElochHat 0.1723 04/25/14 TN14 48 LRMerwin 0.9626 LRCedar 0.0365 05/02/14 TN14 49 EFLewisR 0.7314 GermCr 0.1153 LRMerwin 0.0757 Cowman 0.0478 LRCedar 0.0221 04/25/14 TN14 50 LRMerwin 0.9357 LRCedar 0.0434 GermCr 0.0096 Cowman 0.0069 04/25/14 TN14 51 LRMerwin 0.7049 MillCr 0.0998 LRCedar 0.0925 LRhatW 0.0423 GermCr 0.0234 APPENDIX B 5

DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 04/25/14 TN14 52 LRCedar 0.7201 KalamSu 0.1165 SandyR 0.0934 LRMerwin 0.0234 EFLewisR 0.0164 04/25/14 TN14 53 LRMerwin 0.8052 GermCr 0.1754 KalamW 0.0083 MillCr 0.0034 04/25/14 TN14 54 LRMerwin 0.9028 LRCedar 0.0964 04/25/14 TN14 56 LRMerwin 0.7704 LRCedar 0.1973 GarysR 0.0227 04/25/14 TN14 57 LRCedar 0.4862 Cowman 0.318 LRMerwin 0.1412 HoodR 0.0223 LRhatW 0.011 04/25/14 TN14 58 LRMerwin 0.9905 04/25/14 TN14 59 LRMerwin 0.5357 LRCedar 0.453 MillCr 0.0041 05/02/14 TN14 60 LRMerwin 0.8184 LRCedar 0.163 GermCr 0.0148 03/20/14 URT14 125 LRMerwin 0.6158 LRCedar 0.3646 SFTout 0.0164 03/20/14 URT14 171 LRCedar 0.7 LRMerwin 0.2745 KalamW 0.0172 03/20/14 URT14 176 LRCedar 0.6964 LRMerwin 0.158 Cowman 0.0657 GermCr 0.0292 KalamW 0.0191 03/31/14 URT14 194 LRCedar 0.876 GermCr 0.0821 LRMerwin 0.0184 KlickSu 0.016 03/31/14 URT14 198 LRMerwin 0.5204 LRCedar 0.3855 LRhatW 0.0372 GrRLC 0.0189 ElochR 0.0179 03/31/14 URT14 212 LRMerwin 0.6888 LRCedar 0.298 LRhatW 0.0081 04/05/14 URT14 237 LRCedar 0.368 GarysR 0.3447 LRMerwin 0.1755 GrRLC 0.0347 KalamW 0.0233 04/05/14 URT14 245 LRMerwin 0.862 LRCedar 0.0919 EFLewisR 0.0191 GarysR 0.0139 ElochR 0.0046 04/05/14 URT14 251 LRCedar 0.3575 GermCr 0.2228 LRMerwin 0.1653 EFLewisR 0.0816 GarysR 0.0451 04/05/14 URT14 254 LRMerwin 0.3763 LRCedar 0.2692 ElochR 0.186 GermCr 0.1077 MillCr 0.0389 04/05/14 URT14 255 LRMerwin 0.7479 Cowman 0.1063 LRCedar 0.0956 KalamW 0.0265 ElochR 0.014 04/05/14 URT14 256 LRMerwin 0.76 LRCedar 0.2369 04/05/14 URT14 265 LRCedar 0.6136 LRMerwin 0.347 GarysR 0.0167 ElochHat 0.0071 MillCr 0.0053 04/05/14 URT14 275 LRMerwin 0.5198 EFLewisR 0.2367 MillCr 0.0541 GrRLC 0.0512 SandyR 0.0371 04/05/14 URT14 276 LRCedar 0.5596 LRMerwin 0.4314 04/11/14 URT14 277 LRMerwin 0.6746 GrRLC 0.2292 GermCr 0.0536 LRCedar 0.024 Cowman 0.0072 04/11/14 URT14 282 LRMerwin 0.8601 LRCedar 0.1004 MillCr 0.0248 Cowman 0.0062 04/11/14 URT14 285 LRCedar 0.6781 LRMerwin 0.2718 GermCr 0.0322 Cowman 0.0054 KalamW 0.0039 04/18/14 URT14 298 LRMerwin 0.9377 LRCedar 0.0401 Cowman 0.0212 04/18/14 URT14 299 LRMerwin 0.6643 LRCedar 0.278 LRhatW 0.0341 ElochHat 0.0087 Cowman 0.0044 04/18/14 URT14 300 LRMerwin 0.3298 LRCedar 0.2814 ElochR 0.134 GarysR 0.1246 GermCr 0.0671 04/18/14 URT14 306 LRMerwin 0.9662 LRCedar 0.0308 04/25/14 URT14 308 LRCedar 0.502 GermCr 0.237 ElochHat 0.1357 LRhatW 0.0664 GarysR 0.0496 APPENDIX B 6

DATE CODE PRIMARY P SECONDARY P TERTIARY P 4TH P 5TH P 04/25/14 URT14 309 MillCr 0.5049 Cowman 0.2334 LRMerwin 0.0913 LRCedar 0.0722 KalamSu 0.0685 04/25/14 URT14 310 LRMerwin 0.4623 LRCedar 0.3679 Cowman 0.1637 04/25/14 URT14 311 LRCedar 0.4227 GrRLC 0.3982 LRMerwin 0.133 GermCr 0.0231 KalamSu 0.0076 05/02/14 URT14 312 LRMerwin 0.9763 GermCr 0.0072 Clack 0.0034 GarysR 0.0033 05/02/14 URT14 313 LRMerwin 0.5704 LRCedar 0.4053 EFLewisR 0.0087 Cowman 0.0078 05/02/14 URT14 314 LRCedar 0.6174 LRMerwin 0.2157 GermCr 0.0661 Cowman 0.0565 ElochHat 0.0268 05/02/14 URT14 315 LRCedar 0.6238 LRMerwin 0.345 Cowman 0.0225 05/02/14 URT14 316 Clack 0.7601 LRCedar 0.1006 LRMerwin 0.0773 Cowman 0.059 05/02/14 URT14 317 LRMerwin 0.8755 LRCedar 0.0725 Cowman 0.0516 05/02/14 URT14 318 LRMerwin 0.6856 LRCedar 0.3082 03/14/14 URT 66 LRMerwin 0.7845 LRCedar 0.2056 03/14/14 URT 77 LRCedar 0.7485 GermCr 0.2196 GarysR 0.0151 GrRLC 0.0059 ElochR 0.0058 APPENDIX B 7

Appendix C Wild Winter Steelhead Spawning Log 2014

Spawn Date Total Egg Weight w/o ovarian Estimate Eggs/Female Eyed Egg Eggs/LB Eyed Eggs On-Hand Dead Eggs Actual Eggs/Female Percent of Egg Loss Estimate # Fry Ponded Comments 4/7/2014 397 2706 2434 2756 25 2781 0.90% 2728 4/7/2014 422 2876 2221 3856 285 4141 6.88% 3817 4/7/2014 769 5242 2676 2725 278 3003 9.26% 2698 Ponded 6/6 4/11/2014 640 4362 2413 3785 46 3831 1.20% 3747 4/15/2014 844 5753 2569 5530 268 5798 4.62% 5475 4/21/2014 56 382 193 191 384 49.74% 191 Added to TN 14-32 4/21/2014 381 2597 2583 2223 16 2239 0.71% 2201 4/21/2014 530 3612 2456 3222 93 3315 2.81% Plant NF Lewis 4/21/2014 489 3333 2406 2800 9 2809 0.32% 2772 4/24/2014 623 4246 2596 3998 46 4044 1.14% 3958 4/25/2014 720 4908 3234 4948 253 5201 4.86% 4899 4/29/2014 630 4294 2294 3143 450 3593 12.52% 3112 4/29/2014 551 3756 2430 3086 208 3294 6.31% 3055 4/29/2014 424 2890 2868 2839 247 3086 8.00% 2811 5/2/2014 924 6298 3000 6431 518 6949 7.45% 6367 5/2/2014 1043 7109 2293 5874 213 6087 3.50% Plant NF Lewis 5/2/2014 600 4090 2661 4084 51 4135 1.23% 4043 5/2/2014 375 2556 2860 2876 114 2990 3.81% 2847 5/9/2014 819 5582 2227 3802 758 4560 16.62% 3764 5/9/2014 395 2692 2556 1331 1397 2728 51.21% 1318 Added to TN 14-56 5/9/2014 749 5105 2515 4051 95 4146 2.29% 4010 5/9/2014 682 4649 2820 4200 178 4378 4.07% 4158 5/9/2014 758 5167 2932 4685 803 5488 14.63% 4638 5/16/2014 640 4362 2220 3566 64 3630 1.76% 3530 5/16/2014 629 4287 2347 3818 120 3938 3.05% 3780 5/16/2014 467 3183 2914 3411 105 3516 2.99% 3377 95315 2581 93233 6831 100064 6.83% -83296 APPENDIX C 1

APPENDIX D Wild Winter Steelhead Spawning Crosses 2014

SPAWN DATE CROSS NO. 4/7/2014 1 4/7/2014 2 4/7/2014 4/11/2014 4/15/2014 4/21/2014 4/21/2014 4/21/2014 4/21/2014 4/24/2014 4/25/2014 4/29/2014 4/29/2014 3 4 5 6 7 8 9 10 11 12 13 FEMALES (DNA No. and Assignment Probability) MALES (DNA No. and Assignment Probability) TN 14 4 TN 14 5 LRMerwin.6138 LRCedar.3559 LRMerwin.2145 MT 14 1 CC 14 20 LRMerwin.3813 LRCedar.1043 LRMerwin.7812 TN 14 13 TN 14 2 LRCedar.5232 LRMerwin.1899 LRMerwin.9721 TN 14 30 TN 14 21 LRCedar.9081 LRMerwin.9969 MT 14 2 TN 14 19 LRMerwin.909 LRMerwin.7458 TN 14 15 MT 14 5 LRMerwin.9928 LRMerwin.9088 TN 14 32 TN 14 6 LRMerwin.9264 LRMerwin.6853 MT 14 6 TN 14 43 Mill Cr..5049 LRMerwin.985 TN 14 41 TN 14 22 LRMerwin.7531 LRMerwin.4237 LRCedar.3772 MT 14 7 TN 14 68 LRCedar.5262 LRMerwin.4588 LRCedar.4302 LRMerwin.3456 TN 14 60 TN 14 59 LRMerwin.8184 LRMerwin.5357 MT 14 3 TN 14 18 MT 14 4 LRMerwin.9068 LRMerwin.9299 LRCedar.8996 TN 14 57 LRMerwin.1412.4862 LRCedar MT 14 3 MT 14 4 LRMerwin.9068 LRCedar.8996 APPENDIX D 1

SPAWN DATE 4/29/2014 5/2/2014 5/2/2014 5/2/2014 5/2/2014 5/9/2014 5/9/2014 5/9/2014 5/9/2014 5/9/2014 5/16/2014 5/16/2014 5/16/2014 CROSS NO. 14 15 16 17 18 19 20 21 22 23 24 25 26 TOTAL INDIVIDUALS FEMALES (DNA No. and Assignment Probability) MALES (DNA No. and Assignment Probability) TN 14 4 TN 14 5 TN 14 17 MT 14 3 MT 14 4 LRMerwin.9488 LRMerwin.9068 LRCedar.8996 TN 14 69 MT 14 9 LRMerwin.9712 LRMerwin.9349 MT 14 10 TN 14 62 Grays R..498 LRMerwin.6964 MT 14 7 TN 14 68 LRCedar.6874 LRCedar.4853 LRMerwin.4728 TN 14 31 TN 14 63 LRCedar.5404 LRMerwin.8515 TN 14 56 MT 14 11 LRMerwin.7704 LRMerwin.496 LRCedar.2467 TN 14 29 TN 14 65 LRMerwin.5953 LRMerwin.9094 TN 14 77 MT 14 15 LRMerwin.6625 LRCedar.3059 LRMerwin.9817 TN 14 78 TN 14 73 LRMerwin.763 LRCedar LRMerwin.6858.2302 LRCedar.2403 MT 14 18 MT 14 12 LRMerwin.6908 LRCedar LRCedar.5175.223 LRMerwin.4637 MT 14 13 TN 14 41 LRMerwin.6656 LRCedar.101 LRCedar.3651 LRMerwin.2634 MT 14 16 TN 14 34 LRMerwin.9945 LRCedar.567 LRMerwin.3762 MT 14 21 MT 14 17 LRMerwin.9341 LRCedar.0586 LRMerwin.9402 LRCedar.0355 26 25 APPENDIX D 2

Appendix E Steelhead Redd locations, Lewis River, WA 2014

Steelhead Redd Locations, Lewis River, WA 2014 Surveys Path: G:\work\common\MikeIchisaka\lewis\Lewis_Redds\Lewis_redds022615.mxd 2/26/2015 Steelhead 2014 Source: Esri, DigitalGlobe, GeoEye, i-cubed, Earthstar Geographics, CNES/Airbus DS, USDA, USGS, AEX, Getmapping, Aerogrid, IGN, IGP, swisstopo, and the GIS User Community I 0 0.1 0.2 0.4 0.6 0.8 Miles 1:13,000 WASHINGTON Map Sheet 1 of 3 Lewis River 3 2 Content may not reflec Map National Location Geog 1 MERWIN I

Steelhead Redd Locations, Lewis River, WA 2014 Surveys Path: G:\work\common\MikeIchisaka\lewis\Lewis_Redds\Lewis_redds022615.mxd 2/26/2015 Steelhead 2014 Source: Esri, DigitalGlobe, GeoEye, i-cubed, Earthstar Geographics, CNES/Airbus DS, USDA, USGS, AEX, Getmapping, Aerogrid, IGN, IGP, swisstopo, and the GIS User Community I 0 0.1 0.2 0.4 0.6 0.8 Miles 1:13,000 WASHINGTON Map Sheet 2 of 3 Lewis River 3 2 Content may not reflec Map National Location Geog 1 MERWIN I

Steelhead Redd Locations, Lewis River, WA 2014 Surveys Path: G:\work\common\MikeIchisaka\lewis\Lewis_Redds\Lewis_redds022615.mxd 2/26/2015 Steelhead 2014 Source: Esri, DigitalGlobe, GeoEye, i-cubed, Earthstar Geographics, CNES/Airbus DS, USDA, USGS, AEX, Getmapping, Aerogrid, IGN, IGP, swisstopo, and the GIS User Community I 0 0.1 0.2 0.4 0.6 0.8 Miles 1:13,000 WASHINGTON Map Sheet 3 of 3 Lewis River 3 2 Content may not reflec Map National Location Geog 1 MERWIN I