CCMR Lab Safety Room Orientation for Physical Sciences Building Cryo S/TEM Rm. B91 and BioTwin Rm. B71

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CCMR Lab Safety Room Orientation for Physical Sciences Building Cryo S/TEM Rm. B91 and BioTwin Rm. B71 Mariena Silvestry Ramos, PhD PSB B88 Office 607-255-6272 Cell 646-320-6130 ms3289@cornell.edu

Emergency Exits and Evacuation In the event of a building evacuation alarm or other emergency, immediately proceed to the nearest exit as outlined by the red boxes on the map. Exit immediately. There is one exit near B95 (liquid nitrogen storage and holder room) and two exits near the CryoS/TEM and BioTwin rooms. Additionally, the PSB Atrium and/or Clark Hall are places to exit and wait until the emergency has passed and it s safe to return.

Emergency Contact Info In case of emergency, dial 5-1111 from a campus phone. Mobile network signals are mostly inaccessible in the facility and immediate surrounding areas. Two telephones are available for emergency use only: Next to the Cryo S/TEM and BioTwin microscopes For non-emergency, urgent equipment issues, call the appropriate staff member using the number posted on the HASP signs on the doors In the event you have any further questions regarding the proper procedures, safety regulations, or other concerns, please contact one of the Facility Staff. The following contacts may be able to assist you: Keane Leitch, PSC Safety Manager kjl44@cornell.edu, 121 Baker Lab Todd Pfeiffer, PSC Facilities Director tap66@cornell.edu, 105 Weill Hall

Working in the CCMR Cryo S/TEM and BioTwin Labs Safety Training The following laboratory safety courses, offered on-line or in the classroom by Cornell University EH&S, are required to access the CCMR Cryo S/TEM BioTwin Labs. EH&S course numbers follow in parentheses for your reference. Please refer to https://sp.ehs.cornell.edu/training/pages/default.aspx (internal users) or https://sp.ehs.cornell.edu/training/pages/training-access.aspx (external users) for information on how to obtain training or verify your CULearn information. Required for access to the Cryo S/TEM and BioTwin Labs: EH&S training Laboratory Safety: EH&S training in Chemical Waste Disposal: EH&S training in Handling Cryogens: EH&S Radiation Producing Equipment Safety: #2555 Laboratory #2617 Chem Waste #3055 Cryogensfty #5033 EHS-RPE

Rules for using the CCMR Cryo S/TEM BioTwin facilities No wet chemistry is allowed in the lab. All samples must be prepared in your own lab. Do not bring hazardous samples or materials into the lab without prior consent/approval by the Facilities Manager. This is not a storage facility - you must take specimens when you leave. Anything leftover will be promptly disposed. Do not bring food or drinks into B-91 or B-71. Users failing to follow safety rules will be denied access to the lab. Wear proper PPE and follow safety rules when handling liquid nitrogen. Do NOT insert foreign objects into microscope computer (hard drives, thumb drives, chargers, etc); they contain viruses that will take damage the computer that operates the microscope. Ask about data transfer protocols before the start of your session

Instrument and sample holder sign up rules Sign ups for instrument time are done via Coral; users are encouraged to notify manager and/or lab members if sessions are finished early so as to maximize instrument use/time Specialty holders MUST be signed up for via: https://docs.google.com/spreadsheets/d/1_ximuwaj1aagbnsp-ywfkh8eijexpr1nm6-7qlrhx8/edit#gid=1606419089 Any and all issues must be logged on the lab logbook and the Facilities Manager should be notified immediately. Failure to follow these procedures could result in cancellation of access to facilities Lab head/pi is liable for any non wear-and-tear repair costs incurred due to lab member(s) irresponsible handling/operating of holders Consultation with Facilities Manager is required prior to bringing any samples to the lab. Do not introduce new hazards or hazardous materials without express approval from facility staff To be up-to-date about instrument issues or downtime, please send an email to the cryo STEM list: CCMR-CRYO-STEM-L@cornell.edu

CCMR Shared Facility Access Form (more information at http://www.ccmr.cornell.edu/facilities/becoming-a-ccmr-facility-user/) Submit one form to each facility that you are requesting access to. 1) Print out this page. Illegible or incomplete forms may not be accepted. 2) Read the facility orientation completely. Required EH&S safety trainings for individual areas are listed below. Facility Duffield Hall Bard Hall Trainings A,F, and W required for access to all facilities Room Additional Trainings 150 CR Facility Room Additional Trainings F03 (Keck) - 231 (Prep) C Clark All D21, D22 C*G*M*U* Hall D15 (AFM) - B47/A/B C*L*U* D10 C*G*M* B55 G ST Olin B78 C B56 C 1158 CR Snee SB30 L 1102 R Hall SB56 & 58 R* 1149 (µprobe) R* *Required for specific activities, see facility orientation A C F G H L M R U W Safety trainings key Laboratory safety (#2555-laboratory) Cryogen handling (#3055-cryogensfty) Fire safety (#5330-firesafety) Compressed gas (#2335-compressed) Hazard communication (#2343-hazardcomm) Laser safety (#2397-lasersafety) Magnetic fields (#5035-magnetic) Radiation producing equipment (#5033 EHS-RPE) Fume hood (#3355-FumeHood) Chemical waste disposal (#2716-chemwaste) 3) Fill in user information below, sign and bring completed forms to the appropriate facility manager. User Name Cornell University ID card # NetID/E-mail Status Department Advisor By signing below, I am attesting that I have read the facility orientation form and completed the necessary EH&S safety trainings. User Signature Date / / Office use: Door e-mail list entered by