Table Top and Convertible Anesthesia Machine Models: M3000 and M3100 (SHOWN WITH OPTIONAL EQUIPMENT)

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OPERATOR MANUAL Table Top and Convertible Anesthesia Machine Models: M3000 and M3100 M3000 M3100 (SHOWN WITH OPTIONAL EQUIPMENT) 12330 SE Hwy 212 Clackamas, Oregon 97015 (503) 822-1157 Local and International Toll Free 877-615-5632 info@scivenascientific.com www.scivenascientific.com 1

Table of contents Page 3 Product Examination and Preparation for Use Page 4 M3000 Machine Diagram and Part Numbers Page 5... M3100 Stand Model Diagram Page 6-8 M3100 Additional Assembly Page 9 E-Tank Manifold Installation Instructions Page 10..Machine Set-up Instructions Page 11-25 Accessory Options: Multi-Station Manifolds - 12, 13 Induction Chambers 14, 15 Breathing Circuit 16, 17 Rodent Heated Surgical Station with Downdraft 18, 19 Multi-Station Surgical Board 20, 21 Waste Gas Interfaces 22-25 Page 26..Warranty / Return Policy Page 27..Shipping Policy Page 28..Documentation 2

Examination and Preparation for Use Thank you for ordering a Scivena Scientific Anesthesia Innovations anesthesia machine! We are delighted to have you as a Scivena Scientific Anesthesia Innovations customer and want you to be completely satisfied with your purchase. Please inspect the contents of your order to see if everything is as you expected. Should anything not be exactly right or if anything was damaged in shipping, please contact your sales representative right away for help. Our goal is to make your new anesthesia machine as easy to use and care for as possible. This device is meant to be operated under the normal surveillance and control of a veterinarian trained in its use. However, you need to know more about this device than just how to operate it. Please read this manual in its entirety before using the anesthesia machine! If you have any comments or questions, we welcome the opportunity to address them. Please contact us directly at 877 615-5632. Thank you! Brian Lawson President, Scivena Scientific Anesthesia Innovations Designed and Manufactured With Pride In Oregon, USA 3

M3000 Diagram and Part Numbers FIGURE #1 A I F J H E B D G C FRONT BACK A. Main Flow Meter- Indicates precise amount of carrier gas entering the system. B. Flow Control Knob- adjusts precise amount of carrier gas entering the anesthesia machine. C. Flush Valve- flushes pure carrier gas (bypasses the vaporizer) through the system. Flow restricted to 30 LPM for subject safety. D. Optional Vaporizer- vaporizes the anesthetic agent from a liquid to a gaseous state. Delivers anesthetic agent at precise percentage concentration independent of temperature and flow rate. New Tec 3 (p/n VAP3000 Isoflurane, p/n VAP3100 Sevoflurane). E. Common Gas Outlet with 15mm female adaptor. Port through which carrier and anesthetic gas mixture is delivered through the anesthesia system. (See page 14-17 for induction chamber and breathing circuit options). F. Carry Handle G. Carrier gas connection (O2, Med Air or other)- DISS male thread a. NOTE: optional quick disconnect fitting can be installed. Please call for options. H. Optional Waste Gas Interface (p/n EVC630) I. Accessory mounting receptacles (2). J. Optional Balanced Flow Multi-Station Manifold (p/n RES535) 4

M3100 Stand Model Diagram and Additional Assembly: M3000 Anesthesia Machine shown with optional Isoflurane Vaporizer (VAP3050), Waste Gas Interface (ECV630) and Balanced Flow Multi-Station Manifold (RES535) Top Platform Accessory Basket Dual E-Tank Manifold (optional) Center Post (2 round tubes) Five Leg Base 5

Attaching the Base Post (See FIGURE #2) 1. Unpack the 5 leg base and set it on the floor 2. Unpack both 2 round tubes 3. Assemble the base tube onto the base post. 4. Install and tighten the screws supplied into the post. NOTE: The post should fit flush onto the base, if the holes do not align, try the other end of the post. FIGURE #2 6

Attaching the Top Post (See FIGURE #3) 1. Assemble the top post over the bottom post pin 2. Install and tighten the screws supplied into the post. NOTE: The post should fit flush onto the base post, if the holes do not align, try the other end of the post. FIGURE #3 7

Attaching the Top (See FIGURE #4) 1. Assemble the top platform onto the post. 2. Install and tighten the screws supplied into the top post. FIGURE #4 8

Installing the Optional E-Tank Manifold (See FIGURE #5) (P/N MA2000) 1. Align the holes in the E tank manifold with the two holes located in the post. 2. Insert the 2 screws and tighten firmly. NOTE: Do not over tighten the e tank yoke knobs ( T shaped handles) * If the e tank leaks, change the seal. CAUTION! NEVER USE PETROLEUM PRODUCTS ON OR NEAR THE CARRIER GAS SUPPLY WHEN USING OXYGEN AS THE CARRIER GAS! FIGURE #5 9

M3000/M3100 Set Up: 1. Place M3000 on level surface or, for M3100 model, on stand and secured with included pins. 2. Connect selected carrier gas hose to DISS male thread connection on back of the machine (G) and connect hose to carrier gas supply (SEE FIGURE #1). a. Common carrier gases include Oxygen (O2), Medical Air (Med Air), and other combinations of gases. **CAUTION! NEVER USE PETROLEUM PRODUCTS ON OR NEAR THE CARRIER GAS SUPPLY WHEN USING OXYGEN AS THE CARRIER GAS! 3. Connect any optional accessories (see pages 11-25). 4. Connect waste anesthetic gas management system. 5. Fill vaporizer with appropriate anesthetic agent. 6. Make sure carrier gas supply is on. M3000/3100 is now ready for use 10

OPTIONAL ACCESSORY INSTRUCTIONS Balanced Flow Multi-Station Manifolds Induction Chambers Breathing Circuits Multi-Station Procedure Board Heated Rodent Surgical Station Waste Gas Interfaces 11

Balanced Flow Multi-Station Manifold User Instructions RES535-2 Port RES536-4 Port RES537-6 Port The Scivena Scientific Balanced Flow Multi-Station Manifold System is designed so that every port on the manifold gets equal fresh gas delivery independent of the accessory being used. The manifold can accommodate up to six accessory ports. C B D A E F Shown in four port configuration RES356 A. Accessory Receptacle (M3000/M3100) or Mounting Bracket (all other machines) B. Manifold post C. Manifold body with ports. D. Port on/off stopcocks a. Port is ON when toggle is in vertical position. E. Main fresh gas inlet with 15mm female adaptor. F. 15mm Male common outlets (up to six) 12

Set-up: Use: 1. Mount the manifold to the anesthesia machine utilizing the included bracket (A) by inserting the post of the manifold (B) into the bracket and tightening. 2. Connect the main fresh gas inlet adaptor (E) to the common outlet of the vaporizer. 3. Connect your accessories (induction chamber, breathing circuits etc.) to the ports on the manifold body (F) noting which number on the manifold body coordinates with an accessory. **DO NOT TURN ON THE MAIN ANESTHESIA FLOW METER UNTIL AT LEAST ONE STOPCOCK ON THE MULTI-STATION MANIFOLD IS IN THE ON POSITION. Otherwise, pressure can build up in the system which can damage the vaporizer.** 1. Connect and activate the waste anesthetic gas system to your accessories. 2. When ready to use any accessory, open the stopcock to that accessory by turning the toggle to the ON position. 3. With one or more toggle switches in the ON position, it is now safe to turn on the main anesthesia flow meter. 4. The Multi-Port Manifold will now deliver the same rate of fresh gas flow to each open port. a. Example: The main flow meter on the anesthesia machine is set to 3 LPM, and two ports are open on the Multi-Port Manifold, each port will deliver 1.5 LPM fresh gas flow. It is recommended that when the anesthesia machine is not in use, that at least one port on the Multi-Station Manifold remains open. This will prevent accidental pressure build up in the anesthesia system if the main flow meter is turned on. 13

Sealed Induction Chamber User Instructions RES644 3 Liter RES645 5 Liter RES646 7 Liter The Scivena Scientific Sealed Induction Chamber allows for safe and effective anesthetic induction of rodents while protecting the user from waste anesthetic gas exposure. A B D E C A. Chamber lid with airtight seal gasket B. Chamber body with seal clamps C. 15mm female gas inlet (O2/gas or CO2) D. 22mm male waste gas evacuation port a. 19mm EVAC ports available E. Five foot extension hose with 15mm female adaptors 14

Set-Up: Use: 1. Place chamber on level surface. 2. Connect one end of the extension hose to the common outlet of the anesthesia machine, Scivena Scientific Multi-Port Manifold outlet port, or any diverter system with a male common outlet on the end of the tubing, utilizing the 15mm male adaptor on the extension hose (E). 3. Connect opposite end of the extension hose to the 15mm gas inlet on the induction chamber (C). 4. Connect 22mm EVAC tubing to the 22mm male waste gas evacuation port on the induction chamber (D). a. Connect 22mm tubing to waste gas management system. b. **The Sealed Induction Chamber must be used with either a passive waste gas management system, or if using an active waste gas vacuum, a Scivena Scientific Waste Gas Interface (RES505 and EVC630). 5. Remove the lid of the chamber by pulling the blue secure clamps outward and down to release. 1. Make sure the waste anesthetic gas system is in place and functioning. 2. Before turning on any anesthetic gas, place the subject into the chamber and secure the lid using the blue clamps- the chamber is now sealed. 3. Turn on the fresh gas flow using the main flow meter on the anesthesia machine. a. Recommended fresh gas flow for induction chambers is 2 LPM. 4. Turn the vaporizer on to desired concentration. a. Recommended vaporizer concentration for induction chambers is 2-3%. 5. Once subject is anesthetized, turn off the vaporizer leaving the main flow meter on to 2LPM. 6. Using the O2 flush valve on the anesthetic machine, flush pure O2 through the chamber to remove remaining anesthetic gas. Recommended purge times: RES644-3 Liter- 90 seconds RES645-5 Liter- 105 seconds RES646-7 Liter-115 seconds 7. Once chamber is sufficiently purged, turn off fresh gas flow to the chamber, open chamber lid, remove subject and replace chamber lid. 15

Rat and Mouse Non-Rebreathing Circuit User Instructions RES500 The Rat and Mouse Non-Rebreathing Circuit is specially designed to both deliver fresh anesthetic gas to rats and mice, and effectively remove waste anesthetic gases- all in one, complete system. When used properly, the nosecone diaphragm minimizes waste anesthetic gas leaks into the environment- even when using a passive waste anesthetic gas removal system. B C D A A. Fresh anesthetic gas delivery tube with 15mm male adaptor. Connects to common outlet of the vaporizer on the anesthesia machine. B. Fresh anesthetic delivery nosecone body. C. Nosecone diaphragm with ring. D. Waste anesthetic gas outlet for 19mm or 22mm EVAC tubing. 16

Set-Up: Use: 1. Connect the fresh anesthetic gas delivery tube to the common outlet of the vaporizer or Diverter System utilizing the 15mm male adaptor (A). 2. Secure the latex diaphragm (C) onto the nosecone (B) utilizing the included O-ring. a. Stretch the diaphragm material over the end of the face mask, holding it in place with the thumb and forefinger of one hand and reinstall the O-ring over the diaphragm material. b. The O-ring will only fit over the latex material when oriented properly. If the O-ring does not slide on easily, or fit snug, remove the O-ring, flip it over and replace over the latex material. c. The diaphragm is made of 12 mil thickness latex sheet material. This material is thicker and more resistant to tearing and oxidation than surgery glove material (2-3 mil thickness). We do not recommend that you replace the diaphragm with surgery glove material. Surgery gloves are potentially permeable to anesthetic gases allowing the waste gases to escape into the environment. 10 packs of replacement diaphragms are available (P/N RES501). 3. Cut an appropriate sized hole in diaphragm. a. The diaphragm can be cut appropriately using a pair of delicate sharp/sharp scissors. It is vital to the proper operation of the Rodent Nosecone that the diaphragm be cut with the proper sized orifice--appropriate for the size of the subject and the position of the subject. The circular hole in the diaphragm needs to be small enough such that the diaphragm forms a tight seal around the subject's muzzle, but large enough so that it is not too tight and occludes the nares preventing proper spontaneous breathing. Do not cut a cross (+) or an X in the diaphragm for the subject s nose because diaphragm will not seal properly around the subject s muzzle and anesthetic gases will escape into work space. 4. Connect either 19mm or 22mm EVAC tubing to the waste gas outlet of the breathing circuit (D). 5. Connect the opposite end of the EVAC tubing to your waste gas management system. 1. Make sure waste anesthetic gas management system is in place and functioning. 2. Place anesthetized subject s muzzle into the nosecone/diaphragm, making sure the diaphragm seals around the subject s muzzle. 3. Turn on the fresh anesthetic gas flow to the circuit. a. Recommended fresh gas flow rate is 0.5-1.5 LPM at 1-2% isoflurane concentration. 4. When finished with procedure, turn vaporizer and fresh gas flow to zero, and remove subject from the nosecone/diaphragm to recovery area. 17

Rodent Heated Surgical Station with Downdraft Waste Gas Management RES4000 Provides body temperature regulation, fresh anesthetic gas delivery, waste anesthetic gas removal and subject security all in one convenient product. B C D E A G F A. 22mm Waste gas outlet B. Non-Rebreathing Circuit fresh gas inlet C. Non-rebreathing circuit holding block D. Rat and Mouse Non-Rebreathing Circuit E. Procedure platform F. Tie-down attachment G. Downdraft 18

Set-Up and Use: 1. Place Surgical Station on level surface 2. Connect warm water circulating pump to included attachments and turn on- allow platform to warm for approximately 5 minutes. 3. Insert breathing circuit waste gas outlet (D) into holding block (C) as shown in picture above. 4. Attach active vacuum source to waste gas outlet (A) with 22mm EVAC tubing and turn on. 5. Attach Rat and Mouse Non-Rebreathing Circuit fresh gas inlet (B) to fresh gas outlet of anesthesia machine using included 15mm male adaptors. 6. The Surgical Station is now ready to use 19

Multi-Station Surgical Board User Instructions RES3200 The Multi-Station Surgical Board delivers fresh anesthetic gas to up to six rodent subjects and provides a means to effectively remove waste anesthetic gases, all while keeping the anesthetized subjects securely in place. C B A F E FIGURE #1 D A. Six Port Supply Manifold B. Manifold waste gas exit port C. Coaxial Rat and Mouse Non-Rebreathing Circuit (up to six) D. Procedure platform E. Movable tie-down anchors F. Tie-down material 20

Set-up and Use: 1. Place the Surgical Board on a level surface. 2. Place the nosecones (C) into the manifold (A) with the fresh gas inlet tubing on the top surface (as shown in FIGURE #1). 3. Connect the nosecones to the anesthesia machine vaporizer common outlet using Scivena Scientific s Flow Balanced Multi-Station Manifold or any other diverter system that accepts a 15mm male adaptor. 4. Connect 22mm EVAC tubing to the manifold waste gas exit port (B) and connect to an activated charcoal canister or Scivena Scientific Waste Gas Interface if using with an active vacuum source. 5. Place the tie-down anchors (E) in the desired positions. 6. Use the tie-down materials (F) to secure the anesthetized animals in place once they are on the surgical board. 7. If not using all six stations, use included plugs to occlude unused stations. 21

Waste Gas Interface User Instructions EVC630 For Use with Fan Style Waste Gas Evacuation Unit (EVC3000). The interface unit balances the pressure between the anesthesia machine and the active, fan style evacuation unit. A C F B E E D FRONT SIDE A. 19mm Inlet - connects to waste gas outlet of anesthesia machine or accessory. B. Secondary inlet with included cap- connects to waste gas outlet of induction chamber. C. Interface Manifold D. Reservoir Bag E. Room Air Intake F. 22mm Outlet- connects to fan style waste gas evacuation system. 22

Set-up: Use: 1. Mount the interface to the anesthesia machine with included bracket and mounting hardware. 2. Connect blue 19mm EVAC tubing from the 19mm Inlet to the waste gas outlet on the pop-off valve of the anesthesia machine rebreathing head (for rebreathing machines) or the waste gas outlet of the non-rebreathing circuit (for machines without rebreathing head). 3. Connect the 19mm blue EVAC tubing from the secondary inlet to the waste gas outlet of the induction chamber. 4. Connect the clear 22mm EVAC tubing to the Fan Style Waste Gas Evacuation System. 1. Turn the waste gas evacuation unit on. 2. Use the induction chamber to anesthetize the subject. 3. When finished with the induction chamber, remove the tubing from the Secondary Inlet on the Waste Gas Interface and cap off with included cap. 4. Place subject on nosecone and maintain anesthesia as normal. 5. When finished with procedure, turn evacuation unit off. 23

Waste Gas Interface User Instructions RES505 For Use with Pump Style Waste Gas Evacuation Unit (EVC3100). The interface unit balances the pressure between the anesthesia machine and the active, pump style evacuation unit. A B C D A. 22mm Inlet - connects to waste gas outlet of breathing circuit or induction chamber. B. Interface Body with room air intake. C. ¼ Inch ID Hose barb Outlet- connects to pump style waste gas evacuation system or WAGD wall outlet. D. Vacuum stopcock. 24

Set-up: Use: 1. Connect clear 22mm EVAC tubing from the 22mm Inlet to the waste gas outlet on the breathing circuit or induction chamber. 2. Connect ¼ ID EVAC tubing/hose from pump waste gas evacuation unit to the Hose barb Outlet. 1. Turn the waste gas evacuation unit on. 2. Turn the Vacuum Stopcock (D) to the ON position 3. Use the accessory and as normal when Waste Gas Interface is connected inline. 4. When finished with the accessory, turn the anesthetic gas off to that accessory and turn the Vacuum Stopcock in the line to the OFF position. 5. When finished with procedures, turn waste gas evacuation unit off. 25

Warranty / Return Policy Scivena Scientific Anesthesia Innovations warrants that each product or part shall be free from defects in workmanship and materials, under normal use and with appropriate maintenance, for 10 (ten) years from the date of purchase. For plastic, rubber and disposable parts or items, Scivena Scientific Anesthesia Innovation warrants only that each such part and item shall be free from defects in workmanship and materials at the time of delivery. This warranty shall be void for any product that has been altered, defaced or removed from the original Scivena Scientific Anesthesia Innovation product. Scivena Scientific Anesthesia Innovations shall not be liable for any damage, injury or loss arising out of the use of the product, whether as a result of a defect in the product or otherwise, if, prior to such damage, injury or loss, the product was (1) damaged, misused, or misapplied; (2) repaired, altered or modified by persons other than Scivena Scientific Anesthesia Innovations; (3) not installed in strict compliance with the applicable codes and ordinances; (4) not installed by Scivena Scientific Anesthesia Innovations or an authorized Scivena Scientific Anesthesia Innovations dealer. UNDER NO CIRCUMSTANCES SHALL Scivena Scientific Anesthesia Innovations BE LIABLE FOR INCIDENTAL OR CONSEQUENTIAL DAMAGES AS THOSE TERMS ARE DEFINED IN THE UNIFORM COMMERCIAL CODE. All items returned for service or repairs are the responsibility of the customer. Proper packing methods should be used in returning items to Scivena Scientific Anesthesia Innovations. 26

SHIPPING POLICIES 1. Do not sign the Bill Of Lading until you have inspected the box or crate. 2. Examine the box AS it is delivered and BEFORE the truck/driver leaves. 3. If there is any evidence of damage when it arrives, note it in detail with the phrase possible concealed damage on the bill of lading and immediately call the office for instructions before the truck/driver leaves if at all possible. 4. If there is obvious damage such as a hole in the box, a crushed box, etc., refuse the shipment. The product will then go back to the freight company s terminal where they are entirely responsible. 5. Open and inspect your product as soon as possible. DO NOT WAIT. 6. If you find damage, take as many photographs of everything as soon as you can and email them to inquiry@scivenascientific.com 7. Note: unless the following procedures are followed correctly and we are notified within ten (10) days, SCIVENA SCIENTIFIC LLC cannot accept any responsibility for the problems that may ensue. DO NOT RETURN ANY DAMAGED GOODS TO SUPERA LLC WITHOUT PRIOR AUTHORIZATION OF SCIVENA SCIENTIFIC, LLC AND THE CARRIER. KEEP ALL PACKAGING! DO NOT RETURN ANY DAMAGED ITEMS UNTIL SHIPPING INSTRUCTIONS ARE RECEIVED. ALL CLAIMS MUST BE FILED WITHIN TEN (10) DAYS OF RECEIPT OF GOODS. IF YOU HAVE ANY QUESTIONS CONTACT SCIVENA SCIENTIFIC DIRECTLY AT 877-615-5632. 27

DOCUMENTATION Date purchased: Purchased from: Machine serial number: Vaporizer serial number: Machine service information: Service Date: Service information: Service Date: Service information: Service Date: Service information: 28