Macro-Infusion Guide

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115v and 12v System Macro-Infusion Guide Macro-Infusion rapidly introduces a large volume of solution directly into a tree s vascular system. Macro-infusion delivers complete and even distribution of solution throughout the canopy which provides for predictable results. Be certain to read ALL the instructions covered in this application guide. Refer to the appropriate product guide and product label for dosage and mixing instructions. for protocols, product guides, and technical support 1-877-272-6747 www.treecarescience.com Rainbow Treecare Scientific Advancements 11571 K-Tel Drive Minnetonka, MN 55343

Equipment Checklist Equipment Recommendation or Use Macro-infusion pump* 115v plugs into outlet, 12v pump clips to a marine battery 75 injection tees* Use 1.5 tees for every 1 DBH. (Example: a 30 DBH tree would require 45 macro-infusion tees) 3 connector tees* To connect plastic tubing into multiple pathways 110 of tubing* - 74 one foot lengths, To create the harness between the infusion tees 2 ten foot lengths 1/4 inside diameter - 3/32 wall size 1 four foot section 2 six foot sections 2 15/64 high-helix drill bits* Replace every 5 trees for best uptake and chemical distribution Reservoir For containing solution. 55 gallon trash cans work well. 100-300 feet of extension cords For plugging in drill and/or 115v pump (if necessary) Deep cycle marine battery For powering 12v pump (if necessary) Electric drill Rainbow recommends 18 volt models Wire or straightened paper clip For unclogging tees DBH measuring tape For measuring a tree s diameter Small hammer To lightly tap leaking tees Spade For digging out the root flares Hand trowel or V-shaped hoe For pulling soil away from root flares Hand brush To clean soil off root flares Rake and broom For clean-up *These items are all included in our Macro-infusion Kits (#5305 115v Kit, #5306 12v Kit) macro-infusion pump w/ hardware #5303 115v #5304 12v infusion tees #5311 connector tees #5312 Replacement Parts tubing #5310 drill bits #5314 2 Item# Item Description 5301 115v Macro-infusion pump only 5302 12v Macro-infusion pump only 5309 Supplemental Kit (110 feet of tubing, 75 injection tees, 3 connector tees, 2 high helix drill bits) 5310 Tubing (110 feet) 5311 Infusion Tees (75 pack) 5312 Connector Tees (3 pack) 5313 Pressure Gauge 5314 15/64" High Helix Drill Bits 5381 Screen Filter 5382 Rubber O-ring for filter system

Equipment Assembly Tools Needed: Hose cutter or knife Tape measure Bucket of warm water to primary supply tubing pressure gauge pressure regulator overflow hose (clear hose) Assembly Instructions: 1) Cut tubing into the following lengths: a) 74 1 foot sections (harness assembly) b) 2 10 foot sections (secondary supply tubes) c) 1 4 foot section (primary supply tube) d) 2 6 foot sections (bypass tubes) 2) Assemble harness a) Place tubing in hot water to soften. Insert the infusion tees into the 1 foot lengths of tubing. Make sure to get the tubing over at least the first two ribs of the tees. Be careful not to puncture the tubing. b) Attach all of the 1 foot lengths of tubing together with infusion tees to create the harness. The harness sections can be assembled or broken down to any size lengths depending on the diameter of the tree. It is easier to work with several smaller sections rather than one long section when setting up a tree for macro-infusion. filter power input (115v to outlet, 12v clips to battery) intake hose (colored hose) 3) Assemble supply tubes to the pump a) Attach 4 foot section of tubing (primary supply) to the brass barb on pump. b) Attach connector tee to the opposite end of the primary supply tube. c) Attach the two 10 foot sections (secondary supply tubes) to the connector tee at the end of the primary supply tube. d) Attach a connector tee to the opposite end of each secondary supply tube. secondary supply tubing (10 section) RESERVOIR to pump TREE primary supply tubing (4 section) harness tubing (1 sections) 3

Macro-infusion Protocol Step 1: Inspecting the tree Inspect tree for girdling roots, root rot, or any other sources of stress that may be affecting tree health. Determine how much root flare excavation is needed to make the infusion sites 4-8 inches below the crest of the root flare. (Fig. 1) Not all trees require excavation if the root flares are visible. DO NOT treat trees with significant rot or girdling roots. Inspect canopy for significant canopy die back or stress. This may compromise uptake time and distribution. Fig. 1 Step 2: Determining dosage Refer to the appropriate product guide or product label to determine dosage. Step 3: Excavating the root flares Begin filling reservoir with required amount of water. Use a shovel or trowel to remove sod and soil without damaging the tree. (Fig. 2) Thoroughly brush soil from root flares using a coarse brush. (Fig. 3) Soil left on the root flare can dull the bit, clog the holes, and increase uptake time. Fig. 2 Step 4: Drilling the infusion sites Use a clean, sharp, 15/64 diameter, HIGH HELIX drill bit. (Change drill bit every 5 trees to ensure sharpness) Drill perpendicular to the surface of the flare and DO NOT spin the bit in the hole unnecessarily. (Fig. 4) Spinning the bit will cauterize the vascular tissue resulting in slower uptake. Drill holes through the bark about 1 inch into healthy xylem tissue. (Depth will vary depending on bark thickness) Injection site depth must be adequate to deliver the product into the active xylem tissue. (Fig. 5) Use 1.5 infusion sites for every DBH inch (approximately 1 infusion site every 4-6 inches) evenly spaced around the root flares. Place at least one infusion site on EACH root flare DO NOT place infusion sites into or below dead tissue DO NOT drill into deep valleys or sunken areas Fig. 3 4 Fig. 4

Macro-infusion Protocol xylem Step 5: Inserting tees and connecting the harness Check each tee to be sure it is not plugged and replace badly damaged tees. Firmly insert tees by hand into all infusion sites to form a continuous harness around the tree. (Fig. 6) Don t push or pound tees too deep! Open two sections of the harness on opposite sides of the tree. (Fig. 7) Attach the supply tubing from the pump to feed into the harness in these two locations. Fig. 5 Step 6: Starting the infusion Always begin infusion with water only. DO NOT submerse the pump in water. Only the intake and overflow hoses need to be put into the reservoir. Prime the pump by pouring water into the intake hose until it is full. Submerse the hose into the reservoir immediately, preventing any air from entering the hose. Start the pump by connecting it to a power source. A 12V pump should be connected to a deep cycle marine battery that is optimized for low-power. Connect the red wire to the positive, or red battery terminal. Connect the black wire to the negative, or black battery terminal. Fig. 6 open harness Fig. 7 Fig. 8 to pump pull tee pull tee open harness Step 7: Mixing the product Turn pump off. Add product and remaining water. Start infusion again. Step 8: During the infusion Maintain pressure at 15-20 psi using the pressure regulator. Monitor infusion sites for leaks. Lightly tap any leaking tees with a small hammer. If an infusion site continues to leak, drill a new hole or bypass it with a longer piece of tubing. Pack other equipment such as drill, unused chemical, etc. Prepare other trees on site for treatment. Step 9: Cleaning up After all the solution has emptied and air is drawn into the harness, turn the pump off. Disconnect the supply tubes from the harness. Always keep the supply tubes connected to the pump. Remove tees from the tree and disconnect the harness in 6-10 sections for easier access next time. Replace soil and sod around the base of the tree. DO NOT treat drill holes with wound paint or other sealing compounds 5

Troubleshooting Slow Uptake Use high quality water. Water of poor quality (dirty, high ph, etc) may significantly compromise uptake. Make sure the infusion is done on the root flares. If not, distribution and uptake will be compromised. Make sure the tees are not clogged from dirt and grit. DO NOT pound tees in too far because this will block the flow of solution to the active xylem. Check your drill bit. Use only clean, sharp, high helix drill bits. Dull drill bits may cauterize the xylem and result in slow uptake. Replace bits every 5 trees for the best uptake. Water the tree a day before the infusion if drought stress is a problem. Drought stress may cause trees to transpire less. Maintain adequate soil moisture throughout the growing season. Make sure your spacing of injection sites is correct. Spacing should be 4-6 apart. Spacing over 6 will compromise distribution and uptake. Make sure proper dosing reductions were considered for major losses to the canopy size. Uptake may be compromised and phytoxicity may result without proper reductions. Follow dosing considerations as noted on product label or product guide. Check for large air bubbles in the harness. Pull a tee near the air bubble to release. Large air bubbles will compromise distribution and uptake. Keep pressure between 15-20 psi. Leaking Check to see if any tees are broken The hole may have been drilled into dead wood. Re-drill higher up on the flare. If leaking persists, use a longer piece of tubing to bypass that infusion site. Look closely at w the leak is coming from. It may be coming from another hole in the tree such as a previous year s infusion site. Plug a leaking infusion site by connecting a 12 harness section to both ends of a tee to create a loop as shown. No Pressure Make sure the rubber o-ring seal inside the filter housing is present. Rubbing a small amount of petroleum jelly on the o-ring can help create a better seal. If this still doesn t work, replace o-ring. Check the tip of the intake hose for debris or suction against the reservoir. Maintenance and Storage Do not store equipment in a vehicle for a prolonged period of time. High or low temperatures reduce longevity. At the end of each application, make sure to flush water through the pump and harness to prevent build up of chemical in the system. Do not store pump in temperatures below 41 F/5 C Do not run the pump dry. Prolonged dry operation will overheat and damage the pump. If the pressure gauge shows a pressure when t should be none, vent the gauge by perforating the rubber seal at the top of the gauge to equalize the atmospheric pressure. When testing the pump do not be concerned that the pressure gauge does not rise above 8-10 psi. This is due to the lack of resistance when the harness is not connected to a tree. To check the pressure gauge, cinch the end of the tubing to restrict the flow of water through the pump. Warning: Failure to follow these guidelines could result in pump malfunction. 6